Catalog No. 64-0353 Model SC-20 Dual In
Contents
1. Dual In Line Heater Cooler Catalog No 64 0353 Model SC 20 WARNER INSTRUMENTS A Harvard Apparatus Company Publication 5706 001 REV A WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will co2. E LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICU LAR USE OR OF ANY OTHER NATURE Some states do not allow this lim itation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact your local distributor or Harvard Apparatus Inc 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 valid only in the U S outside U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authori zation number has been issued by our customer service department The cus tomer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original war ranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state In addition we can be reached by e mail at supportewarneronline com or through the web at www warneronline com Publication 5706 001 REV A www warneronline com Dual In Line Heater Cooler Mo
3. ble assembly which allows for monitoring of the actual bath temperature and 3 meters each of PE 160 and x OD Tygon tubing Publication 5706 001 REV A www warneronline com Dual In Line Heater Cooler Model SC 20 Warner Instruments Setup A general configuration for the SC 20 is shown below Note that the illustration here makes use of a PHC Series Imaging Chamber Heater Cooler Jacket to provide additional thermal control for the imaging and recording chamber The SC 20 has two input ports and two corresponding output ports for fluid flow to the sample chamber These lines are attached to the INPUT and OUTPUT PERFUSION PORTS and are identified above The two WATER JACKET INPUT OUTPUT PORTS are the larger diameter ports on the input side of the SC 20 and are also identified The WATER JACKET PORTS are provided for connecting to the INTEGRAL WATER JACKET within the SC 20 NOTE The use of the INTEGRAL SC 20 WATER JACKET is mandatory regard less if the unit is being used for heating or cooling If you look closely at the routing of the two perfusion flow lines exiting the SC 20 the two lines enter into the Series 20 Chamber and Platform on the right hand side of the picture you will see that the upper perfusion line enters into the chamber s input port This line carries your experimental perfusion solution and would come from your solution reservoir The lower perfusion line in the picture enters into the PHC Ser
4. del SC 20 Warner Instruments Warranty amp Service Conto Service Notes Please refer all guestions regarding service to our Engineering Department A Should service be reguired please contact the factory The problem may often be corrected by our shipping a replacement part Factory service if reguired will be expedited to minimize the customer inconvenience B Instruments are inspected immediately upon receipt and the customer is notified if the repair is not covered by the warranty Repairs can often be completed in 1 2 days from our receipt of the instrument C If factory service is required please observe the following instructions 1 Package the instrument with at least 3 inches of cushioning on all sides Use the original shipping carton if it is available 2 Insure the shipment for its full value 3 Include with the shipment an explanation of the problem experienced IMPORTANT Customers Outside of the U S Please be sure to contact us before return shipping any goods We will pro vide instructions so that the shipment will not be delayed or subject to unnecessary expense in clearing U S Customs Publication 5706 001 REV A www warneronline com
5. ies Imaging Chamber Heater Cooler Jacket This line carries heated chilled water comes from a circulating reservoir and is used to aid to maintain the temperature of the chamber platform Tubing Connections Solution Flow Lines PE 160 tubing or any other tubing with a 1 5 mm ID is used to con nect to both of the available input and output solution ports on the SC 20 Connections are made by press fitting the tubing onto the 18 gauge stain less steel hypodermic tubes Cap plugs are supplied to block unused inputs NOTE Use a short length of PE tubing between the heater output and perfusion chamber Publication 5706 001 REV A www warneronline com Dual In Line Heater Cooler Model SC 20 Warner Instruments Setup Cont d Tubing Connections Cont d Water Jacket Flow Line Water is circulated through the SC 20 WATER JACKET via the two large ports located on the input side of the heater cooler Run Tygon flow lines in a loop from your water source a sink or water bath to both ports the SC 20 Connections are made to the SC 20 by press fitting the tubing onto the large bore stainless steel tubes Use of the INTEGRAL WATER JACKET to remove excess heat from the internal Peltier device is critical to the function of the SC 20 and its use is mandatory PHC Series Imaging Chamber Heater Cooler Jacket The use of a separate water jacket attached to a Series 20 chamber to aid in maintaining the temperature of the chamber
6. mply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 7 Dual In Line Heater Cooler Model SC 20 Warner Instruments Table of Contents Introduction ee ee ee KAREE ERGE Re EER 2 General se ER GE id 2 SJT EE EE ON 3 4 Tubing Connections unne we ee 3 Solution FIOW LIMOS ai eiiean E AEE 3 Water Jacket Flow Line see eek ee eke Ge ek Ge Ge Re Ge RR Ge ee 4 PHC Series Imaging Chamber Heater Cooler Jacket ee se ek ee Ge ek GR Re RR Ge Ge Ge ee es 4 Component Placement varslene 4 Op r ti EE ee ee 5 Using with a Chamber see ee Ge ee ek Ge ee ek Ge ek ed 5 OUIGASSING ses EE EE ia aa 5 ADP ae AE 6 Ma intenaNG OR AO EA 6 SPECIES AS SR SE GE ED N Ge ie 6 Warranty amp Service siese ae eke ik ek ke eg ee 7 8 MEER ET ME E E 7 Serce EE Er ie AET 8 Publication 5706 001 REV A www warneronline com Dual In Line Heater Cooler Model SC 20 Warner Instruments Introduction The SC 20 Dual In Line Solution Heater Cooler from Warner Instruments is a versatile and simple to use thermal control device In addition in line solution heating has proven to be one of the most effective methods of maintaining the temperature of perfusion solutions The SC 20 uses reliable and precise Peltier technology to regulate one or two solutions above or below ambient levels Solution temperatures can be maintained at 0 C at flow rates of 2 ml min 5 C at 5 ml mi
7. n or as high as 50 C at 5 ml min An integral water jacket using running water from a tap or large reservoir is used to remove excess heat from the Peltier electronics within the SC 20 Cooling flow rates as low as 4 liters per hour are sufficient to maintain thermal efficiency of the device The SC 20 can be used to control either one or two discrete perfusate solu tions or with a solution gas combination When coupled with a PHC Series Imaging Chamber Heater Cooler Jacket the SC 20 provides an effective means for temperature control in a Series 20 Imaging and Recording Chamber even in the absence of solution flow THIS EQUIPMENT IS NOT DESIGNED NOR INTENDED FOR USE ON HUMAN SUBJECTS N caution Not for clinical use on human patients General In line solution heating is the simplest and most effective method of warm ing or cooling perfusion solutions prior to use The minimal dead space man ifold at the heater cooler output allows it to be used in applications where from 1 to 2 perfusion lines are connected to a chamber or other device The SC 20 Dual In Line Solution Heater Cooler is designed for use with the Warner CL 100 Bipolar Temperature Controller The compact design of the SC 20 makes it possible to install the device immediately adjacent to the input port of a perfusion chamber insuring minimal heat loss The SC 20 will easily accommodate flow rates up to 5 ml min Each heater cooler is supplied with a TA 29 thermistor ca
8. nual and adjusts the SET TEMPERATURE of the system Start the solution flow to the SC 20 INTEGRAL WATER JACKET Start solution flow through the SC 20 to the chamber and PHC Series Imaging Chamber Heater Cooler Jacket if used Place the TA 29 thermistor into the bath to measure its temperature 6 Adjust the SET TEMP to the desired operating temperature Allow the system to thermally stabilize Monitor the temperature at the point of interest using the TA 29 thermistor Make any temperature adjustments on the CL 100 to compensate for heat loss from the SC 20 to the bath Additional adjustment of the heater set point may be required if any of the following change substantially during an experiment e Solution flow rate e Temperature of solution entering the heater e Ambient room temperature e Air currents around chamber Efforts to minimize these factors will be rewarded Outgassing A common problem with rapid heating of solutions is outgassing The bubbles formed can often cause blockages or disruptions to the flow in the chamber bath An effective solution to this problem is to pre warm the perfusate at the reservoir Warner Instruments carries a full line of Solution Reservoir Heaters designed to work in concert with our In Line Solution Heaters Publication 5706 001 REV A www warneronline com 4 Dual In Line Heater Cooler Model SC 20 Warner Instruments Appendix Maintenance Salt solutions can be c
9. orrosive to metal components and can shorten the life of the heater cooler if left in the unit during storage The SC 20 should be flushed with distilled water and blown dry after each use to eliminate the effects of salt and moisture NOTE Proper maintenance is critical to the life and functionality of the device Specifications Minimum Temperature Maximum Temperature Maximum Flow Rate at 5 C Accuracy Perfusion Lines Internal Dead Volume Water Jacket Ports Controller Physical Dimensions Cable Length Connector Type Weight Warranty Publication 5706 001 REV A 0 C 2 ml min maximum flow 50 C 5 ml min 0 1 C Type 316 Stainless Steel 0 032 ID x 0 062 OD 330 il Type 316 Stainless Steel 0 12 ID x 0 147 OD Model CL 100 Bipolar Controller 21 x 165 mm dia x length 1 9m 15 pin Male D 109g 1 year www warneronline com Dual In Line Heater Cooler Model SC 20 Warner Instruments Warranty amp Service Warranty The model SC 20 is covered by our Warranty to be free from defects in mate rials and workmanship for a period of one year from the date of shipment If a failure occurs within this period we will either repair or replace the faulty component s This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS B
10. represents an improvement in function for systems using an upright microscope Series 20 WATER JACKET water is passed through the SC 20 via one of the solution flow paths and is channeled to the Series 20 water jacket lying under the cham ber Since both the perfusion solution and PHC Series WATER JACKET lines are routed through the same heater cooler their temperature will be main tained to the same value Component Placement 1 Plug the SC 20 into the rear of the CL 100 Bipolar Temperature Controller using the attached cable 2 Attach solution flow lines from the solution reservoir s to the input ports of the SC 20 3 Attach the SC 20 INTEGRAL WATER JACKET flow lines to the SC 20 WATER JACKET PORTS 4 Using a support stand place the SC 20 in a convenient location usually adjacent to the sample chamber 5 Run solution flow lines from the output ports of the SC 20 to the input ports of the perfusion chamber 6 The supplied TA 29 Thermistor Assembly is used to monitor the temperature at a point of interest Plug the TA 29 into the MONITOR TEMP IN BNC on the CL 100 and place the thermistor at the point of interest Publication 5706 001 REV A www warneronline com Dual In Line Heater Cooler Model SC 20 Warner Instruments Operation Using With A Chamber 1 2 Turn power to the CL 100 on Set the CL 100 MODE SELECTOR SWITCH into auto the INTERNAL EXTERNAL SELECTOR SWITCH to internal see CL 100 user s ma
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