Class II, Type A2 Biological Safety Cabinets
Contents
1. able Wo 0 45 m 4 8 sq ft 0 6 m 6 5 sq ft 0 75 m 8 1 sq ft 0 9 m 9 7 sq ft ested Ope 229 mm 9 229 mm 9 229 mm 9 203 mm 8 O g Ope 274 mm 10 8 274 mm 10 8 274 mm 10 8 248 mm 9 8 Average A Inflow 0 53 m s 105 fpm e Downflow 0 35 m s 70 fpm 0 35 m s 70 fpm 0 35 m s 70 fom 0 33 m s 65 fom Inflow 424 m h 251 cfm 555 m h 328 cfm 686 m h 406 cfm 724 m h 426 cfm Downflow 63 709 m h 424 cfm 928 m h 555 cfm 1147 m h 686 cfm 1289 m h 759 cfm Exhaust 3796 424 m h 251 cfm 555 m h 328 cfm 686 m h 406 cfm 724 m h 426 cfm Airflow Vo Required Exhaust With Optional Thimble 529 m h 311 cfm 764 m3 h 450 cfm 1116 m h 657 cfm 1164 m h 685 cfm Exhaust Collar Static Pressure For Optional Thimble 32 3A 49 Pa 0 19 in H O 62 Pa 0 24 in HjO 79 eS OSs HO Exhaust Collar PA E gt 99 999 for particle size between 0 1 to 0 3 microns per IEST RP CC001 3 NSF ANSI 49 61 dBA 61 dBA 62 5 dBA 63 dBA O o EN 12469 58 dBA 58 dBA 59 5 dBA 60 dBA Tr p gt 1230 Lux gt 1400 Lux gt 1070 Lux gt 1225 Lux gt 114 foot candles gt 130 foot candles gt 100 foot candles gt 114 foot candles Electrogalvanized steel with Isocide oven baked epoxy polyester powder coating aio O 1 2 mm 0 05 18 gauge Cabinet Full Load e 3 Amps FLA 9A 11A 11 5A TIS A 0 130V A oe P Cabinet BTU 1962 2317 2795 3184 et Weio 243 kg 536 Ibs 22. Optional Exhaust Collar Positions for Thimble Ducting for LA2 Models Side View All Sizes Top View LA2 4A Top View LA2 6A_ Comprehensive Performance Testing At Esco Every Labculture LA2 model manufactured by Esco is individually tested documented by serial number and validated with the following test methods Inflow downflow velocity PAO aerosol challenge for filter integrity Airflow pattern visualization Flectrical safety to IEC61010 1 Additional KI Discus containment and microbiological testing are performed on statistical sampling basis ECCO WORLD CLASS WORLDWIDE General Specifications North America Models USA Canada Mexico 115 V 60 Hz LA2 3A2 1115x852x 1540 mm 43 9 x 33 5 X 60 6 LA2 4A2 1420 x 852 x 1540 mm 55 90 X351 X606 LA2 5A2 0 9 meters 3 1 2 meters 4 1 5 meters 5 1725 x 852 x 1540 mm 67 9 x 33 5 x 60 6 2030 x 852 x 1540 mm 79 9 x 33 5 x 60 6 970 x 623 x 67 0 mm 38 2 x 24 5 x 26 4 1270 x 623 x 670 mm 50 0 x 24 5 x 26 4 1570 x 623 x 670 mm 61 8 x 24 5 x 26 4 1870 x 623 x 670 mm 73 6 x 24 5 x 26 4
3. Labculture Class Il Type A2 Biological Safety Cabinets The Safety Solution for Life Science Laboratories u Qo sm E T EN12469 NSF 49 UL 61010 JIS K3800 SFDA YY 0569 EN 12469 SANS 12469 Labculture Class Il Type A2 Biosafety Cabinet Model LA2 4A_ Es Labculture Multi language display and menu access in 33st French and Spanish ja Quickstart mode to turn the blower and fone on off by moving the sash window to correct position ys RS 232 data output port enables remote monitoring of cabinet operating parameters Unique Esco Dynamic Chamber plenum Negative pressure plenum surrounds contaminated positive pressure plenum no fabric bags are used Dual long life ULPA filters for supply and exhaust per IEST RP CC001 3 with 99 999 efficiency for particle size between 0 1 to 0 3 microns Angled supply filter matches cabinet profile to achieve best downflow uniformity Frameless sash that is easier to clean i os ee h Uu T lt lt Ergonomically angled front improves reach and comfort ie M PT gn Actual work access height is 45 mm 1 8 higher Ee p than tested sash opening Removable one piece work surface simplifies cleaning Nm HIR Eu SS Raised airflow grille maintains safety by preventing blockage Ch Aire a i Esco I5S lt iD E antimicrobial coating on all painted surfaces minimizes
4. Marietta OH USA Melaka Malaysia Mumbai India Philadelphia PA USA Salisbury UK Shanghai China Seoul Korea Delhi India Singapore F E escoglobal com PA O ABI gus Sikes ISSCIBE ba t Esco Micro Pte Ltd PT Esco Bintan Indonesia Cert No 651076 Cert No 651333 E
5. Class Il Type A2 Biological Safety Cabinets Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone Air returns to the common air plenum where the 37 exhaust and 63 recirculation process is continued e The control panel is located on the center of the cabinet and angled down for easy access by the operator e Continuous monitoring of cabinet airflow is displayed on a bright easy to read LCD panel e An integrated temperature compen sated true airflow velocity sensor provides an accurate airflow reading despite room temperature fluctuation e All electronic parts are contained inside a plug and play module that permits easy exchange if required e Microprocessor software updates are available from Esco for download via the Internet Sentinel functions are factory set to default to ON or OFF depending on worldwide destination and local preferences Default settings can be user activated through the touchpad data entry access e Automatic start up sequence will prepa
6. O 0 50 9 i gt E 9 t 0 45 0 40 0 35 Low Inflow Low Downflow 0 325 0 25 0 20 0 25 0 30 0 35 0 40 Downflow Velocity Product Protection Test The test objective is to determine cabinet protection to the product samples inside the cabinet workzone from environmental contaminants e Anebulizer containing 55 mL of 5 to 8 x 106 spores mL B Subtilis is placed at 10 cm 4 inches in front of sash window e Target agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus Subtilis CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet protection from cross contamination of samples placed simultaneously inside the workzone e Anebulizer containing 55 mL of spores 5 to 8 x 10 mL is placed against one of the workzone sidewalls e Target agar plates are placed 36 cm 14 away from the same side wall e Acceptance The number of Bacillus Subtilis CFU recovered on agar plates shall not exceed 2 CFU per test HPV Test Compliant Safer Hydrogen Peroxide Decontamination Compatibility Esco biological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decontaminatable cabinets tested with both BIOQUELL and STERIS patented processes HPV H 0 is a safer and more efficient alternative to conventional Esco cabinets are designed to operate within a performance envelope to maint
7. contamination Enhanced side capture zones optimize containment Work area on Esco 0 9 meter 3 cabinet is equivalent to work area offered on larger The cabinets are Kl Discus tested on sampling basis for performance integrity Available in 0 9 1 2 1 5 and 1 8 meter models 3 4 5 and 6 Shown with optional telescoping stand Js d SFOR EN eaa NSF 49 UL 61010 JIS K3800 SFDA YY 0569 EN 12469 SANS 12469 Esco Labculture has passed more performance tests in more languages for more certifications throughout more countries than any other biological safety cabinet in the world Labculture Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets piee e The ULPA downflow supply filter is tilted proportional to the cabinet front angle to direct more air forward to the front air grille The inflow velocity downflow velocity air flow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front aperture this curtain maintains personnel and product protection even in the unlikely event of a severe inflow or downflow imbalance that would compromise protection in a conventional cabinet Operator Product and Environmental Protection Integrated Filtration System A combination of a supply ULPA filter and an exhaust ULPA filter gives the Labculture cabinet a fully integrated performance envelope for product operator and environmental p
8. laminar flow clean benches and PCR products and instrumentation With the most extensive product line in the industry Esco has passed more tests in more languages for more certifications throughout more countries than any biosafety cabinet manufacturer in the world Esco remains dedicated to delivering innovative solutions for the clinical life science research and industrial laboratory community www escoglobal com Biological Safety Cabinets and Laminar Flow e Laboratory Fume Hoods e Laboratory Ovens Laboratory Incubators e PCR Thermal Cyclers e Microplate Shaker Incubators e Ultralow Freezers ECCO WORLD CLASS WORLDWIDE Esco Technologies Inc e 2940 Turnpike Drive Units 15 16 e Hatboro PA 19040 USA Toll Free USA and Canada 877 479 3726 Tel 215 441 9661 Fax 215 441 9660 us escoglobal com e usa escoglobal com Esco Micro Pte Ltd e 21 Changi South Street 1 Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 mail escoglobal com www escoglobal com gical Safety Cabinet LA2 Brochure A4 vB 08 11 Esco can accept no responsibility for possible errors in catalogues brochures and other printed materials Esco reserves the right to alter its products and specifications without notice All trademarks and logotypes in this material are the property of Esco and the respective companies 9010007 Biolo Esco Global Offices Beijing China Kuala Lumpur Malaysia Manama Bahrain Guangzhou China Hanoi Vietnam
9. radius corners without crevices or joints 21 The cabinet shall have a stainless steel one piece fabricated drain trough with open angles to channel spills to a common drain 22 The closed sidewall shall be sealed without perforations return air slots or concealed areas which can contain contaminants 23 The cabinet shall be free of sharp edges non functional protrusions bolts screws or hardware and all metal edges shall be deburred 24 The cabinet exterior top shall be slanted to discourage placement of foreign objects and to maintain proper exhaust airflow Ergonomics and Convenience 25 The front sash shall be frameless to maximize visibility and accessible for cleaning front and back Sash glass shall be safety glass 26 The sash counterbalance shall be suspended on two high strength cables and the sash shall lock into position in the event one cable becomes detached 27 Magnetic not mechanical proximity sensors shall work in conjunction with the control system to indicate proper sash position for containment 28 Fluorescent lamps shall be mounted behind the control panel module out of the work zone Electronic ballasts shall be used to eliminate flicker extend lamp life and reduce heat output 29 The UV lamp shall operate via an automatic timer with automatic shut off managed by the microprocessor controller and shall be interlocked with the blower motor and fluorescent lights for safety 30 T
10. required Work area containment is maintained even when removable components are lifted out for cleaning The one piece stainless steel work tray edges are radiused and easy to clean without crevices or joints The lower drain trough is a single piece fabrication with wide open angles and a channel to direct spills to the drain The closed sidewall contains no perforations air return slots or other hidden areas where contaminants can accumulate ECCO WORLD CLASS WORLDWIDE Air Quality Filtration Electrical Safety Biosafety Cabinets NSF ANSI 49 USA EN 12469 Europe JIS K 3800 Japan SFDA YY 0569 China ISO 14644 1 Class 3 Worldwide JIS B9920 Class 3 Japan JIS BS5295 Class 3 Japan US Fed Std 209E Class 1 USA EN 1822 H14 Europe IEST RP CCO001 3 USA IEST RP CCOO7 USA IEST RP CC034 1 USA UL C 61010A 1 USA CSA22 2 No 1010 192 Canada EN 61010 1 Europe IEC61010 1 Worldwide Standards Compliance The NSF ANSI 49 certified models are LA2 4A1 LA2 4A2 LA2 4A3 LA2 5A2 and LA2 6A2 Dynamic Chamber Plenum Design I Negative pressure Positive pressure The Esco triple wall design creates a Dynamic Chamber plenum which surrounds contaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure The third wall conceals utilities Comfortable Ergonomic Design The LA2 cabinet is engineered for comfort
11. work surface in clean air The uniform non turbulent air stream protects against cross contamination adjustment Applies to 1 2 meter 4 ft 115 V 60 Hz model contact Esco for information on other models e The permanently lubricated direct drive external rotor motor blower reduces operating costs e Built in RFI and electrical noise filters eliminate interference with adjacent instrumentation e An integral blower hour meter tracks operating life for predictive maintenance planning e To prevent fan damage a paper catch grille traps papers or towels that may drop down on the drain pan preventing them from being pulled into the column by fan suction Sentinel Gold Microprocessor Control Alarm Monitoring System The Esco Sentinel Gold microprocessor based control system supervises operation of all cabinet functions Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right Labculture Esco cabinets use German made ebm papst permanently lubricated centrifugal motor blowers with external rotor designs ntegrated blades narrow the profile and eliminate need for a motor shaft amp Motors are selected for energy efficiency compact design and flat profile The completely integrated assembly optimizes motor cooling amp All rotating parts are unitized and balanced for smooth quiet vibration free operation Biological Safety Cabinet
12. 83 kg 624 Ibs 317 kg 698 Ibs 350 kg 772 lbs oping We 292 kg 644 lbs 345 kg 761 Ibs 410 kg 904 Ibs 486 kg 1072 lbs oping D 1200 x 950 x 1900 mm 1550 x 950 x 1900 mm 1950 x 950 x 1900 mm 2150 x 950 x 1900 mm a A727 Oa a 61 0 x 37 4 x 74 8 76 8 x 37 4 x 74 8 84 6 x 37 4 x 74 8 oola o Ao 3 2 17 m 77 cu ft 2 80 m3 99 cu ft 3 52 m 124 cu ft 3 88 m 137 cu ft Noise reading in open field condition anechoic chamber Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Cabinet BTU Cabinet nominal power x 3 41214 Accessories and Options Cont e Cradle stand electrical hydraulic infinitely adjustable with casters Elevates to accommodate user preference for sitting or standing work surface height When lowered permits movement through standard doorway Note Increases exterior dimensions Electrical Outlets and Utility Fixtures e Electrical outlet Ground Fault Circuit Interrupter North America North America American style Europe Worldwide style DIN 12898 DIN 12919 DIN 3537 Cabinet Accessories e Germicidal UV lamp Controlled by automatic UV lamp timer through Sentinel micro 28 standard size processor control panel e Ergonomic lab chair Emission of 253 7 nanometers for Laboratory grade construction most efficient decontamination meets Class 100 cleanliness alcohol Lamp is p
13. ain protection for personnel product and the environment Airflow parameters used to frame the performance envelope include both inflow velocity and downflow velocity The graph illustrates the boundaries of the performance envelope based on a 1 2 meter 4 ft cabinet as well as the nominal performance point at which tests are conducted The range between high and low Inflow and high and low downflow together with the fluid dynamics achieved through sophisticated cabinet design proportionally sized capture slots and uniform laminar airflow combine to deliver the complete containment and safety solution expected of a professional biological safety cabinet Low Inflow High Downflow 0 32 0 45 For details on the Esco performance envelope contact Esco or your Esco Sales Representative 0 50 m s Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets decontamination using formaldehyde CH 0 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has pungent smell e f there is a gap on the cabinet sealing escaping HPV to the lab will decompose to become oxygen and water Escaping formaldehyde however is harmful to people in the lab Therefore HPV decontamination can be performed with laboratory personnel present inside the lab while formaldehyde decontamination must be performed with no one present in the lab The HPV method improves safety produc
14. anels leading to potentially contaminated and or hazardous areas shall be color coded red 53 All components with the exception of blower motor and ULPA filters shall be located outside of contaminated air spaces to facilitate servicing without the need to decontaminate the cabinet 54 All exterior surfaces shall be painted with a permanent antimicrobial inhibitor coating to minimize contamination ECCO 3 A VWORLD CLASS WORLDWIDE 11 Biological Safety Cabinets Cleanroom Products Compounding Pharmacy Equipment Containment Pharma Products CO Incubators Ductless Fume Hoods In Vitro Fertilization Workstations Lab Animal Research Products Laboratory Fume Hoods Laboratory Ovens and Incubators Laminar Flow Clean Benches PCR Products Ultralow Freezers Since 1978 Esco has emerged as a leader in the development of controlled environment laboratory and cleanroom equipment solutions Products sold in more than 100 countries include biological safety cabinets cleanroom products compounding pharmacy equipment containment pharma products ductless fume hoods in vitro fertilization workstations lab animal research products laboratory fume hoods laboratory ovens and incubators
15. cabinet 11 The supply filter shall be angled and oriented to the 10 cabinet front angle to maximize downflow uniformity over the work surface 12 A removable perforated metal diffuser shall be installed below the supply filter to optimize airflow uniformity and to protect from damage Blower System 13 The cabinet shall have a direct drive permanently lubricated centrifugal blower motor dynamically balanced in two planes compliant to ISO2710 for low noise low vibration and long filter life 14 The blower motor shall have an external rotor design and include an automatic thermal cut out to disable the motor in case of overheating 15 The blower motor shall compensate for filter loading 16 The blower motor system shall be enclosed within a dynamic chamber shaped steel plenum and integrated with the removable supply filter assembly to simplify filter changing 17 The integral damper shall be externally adjustable Cabinet Design Construction Cleaning 18 The cabinet shall be of triple wall design whereby all positive pressure plenums capable of handling contaminated air shall be surrounded by negative pressure No positive pressure areas shall be accessible external to the cabinet The third wall shall conceal utilities 19 The cabinet shall maintain containment performance even when removable work area components are removed for cleaning 20 The work tray shall be one piece removable stainless steel with
16. e exhaust filter media is protected from mechanical damage by an integrated metal screen guard which is absent from conventional HEPA filters and by an angled staggered exhaust damper The top cabinet surface is slanted to discourage placement of objects which could block the exhaust filter and reduce inflow velocity Front Sash Assembly e Integrated sash proximity contacts sense proper sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned The magnetic switch eliminates the chance of wear and tear typical of a mechanical switch The back of the sash can be easily cleaned by removing the sash track cover and swinging up the sash glass The sash is counterbalanced for smooth lightweight operation Esco cabinets use ULPA filters per IEST RP CC001 3 instead of conventional HEPA filters commonly found in biological safety cabinets While HEPA 0 0002 s e oos filters offer 99 99 typical efficiency at 0 3 micron level 0 0 05 0 10 0 15 0 20 0 25 0 30 Particle Size um O Typical Penetration ULPA filters provide 99 99996 typical efficiency for particle sizes of 0 1 to 0 3 micron level 0 40 0 50 ECCO WORLD CLASS WORLDWIDE ULPA filtered air _ Unfiltered potentially contaminated air Room air Inflow air e The counterbalance locking mechanism is inherently
17. et Full Load Amps FLA 3 5 3 5A 4A 4A Cabinet BTU 1347 1651 1921 2023 220 240V AC 60Hz 16 LA2 3A3 LA2 4A3 LA2 5A3 LA2 6A3 Cabinet Full Load Amps FLA 23A 45A 4 7 A 5A Electrical Cabinet BTU 1732 1979 2788 2815 Net Weight 243 kg 536 lbs 283 kg 624 lbs 317 kg 698 lbs 350 kg 772 lbs Shipping Weight 292 kg 644 lbs 345 kg 761 lbs 410 kg 904 Ibs 486 kg 1072 lbs Shipping Dimensions 1200 x 950 x 1900 mm 1550 x 950 x 1900 mm 1950 x 950 x 1900 mm 2150 x 950 x 1900 mm Maximum W x D x H 47 2 x 37 4 x 74 8 61 0 x 37 4 x 74 8 76 8 x 37 4 x 74 8 84 6 x 37 4 x 74 8 Shipping Volume Maximum 2 17 m 77 cu ft 2 80 m 99 cu ft 3 52 m 124 cu ft 3 88 m 137 cu ft Noise reading in open field condition anechoic chamber Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Cabinet BTU Cabinet nominal power x 3 41214 posture e V bar with hooks e Microscope viewing device Adjustable height Stainless steel construction Mounting and viewing pouch Anti skid coating chemical Available for all standard Esco cabinets integrated into sash Factory installed resistant finish specify when ordering ECCO WORLD CLASS WORLDWIDE 10 Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local
18. ge 660 mm or 960 mm 26 or 37 8 e Telescoping height stand for casters nominal range 660 mm or 880 mm 26 or 34 6 Adjustable in 25 4 mm 1 increments The sash window can be lowered further down by removing the arm rest to easily clean the upper back side of the glass Model LA2 Biological Safety Cabinet Technical Specifications O e E E i E LO lt tO el E E r WO Y IL 970 mm 1270 mm 1570 mm 1870 mm L 75mm eec GE Z 7500 761 8 7 73 6 69 l 623 mm 24 5 1115 mm 1420 mm 1725 mm 2030 mm 8 815 mm 32 1 43 9 55 9 67 9 79 9 852 mm 33 5 1 Exhaust ULPA filter 7 Electrical Electronics panel 1 8 meter 4 5 amp 6 models two 15 Safety glass sliding sash window 2 Blower 8 Fluorescent lamp angle outlets di WON kahe 16 Single piece stainless steel back wall 3 Dounon UtPAvdilter ancied 9 Plugged service fixture provisions 11 ain pn single piece work tray and side walls j 12 Stainless steel armrest i i 4 UV light Retrofit Kit provision 2 on each side ici B 17 Removable side panel for plumbing P 10 Electrical outlet Retrofit Kit provision 13 Drain valve Retrofit Kit provision access IV bar Retront Kit provision 0 9 meter 3 model one single 14 Esco Sentinel Gold microprocessor 6 RS 232 port outlet in workzone 1 2 1 5 and control system
19. he cabinet shall be designed with a 10 angled front to optimize user comfort reduce glare and maximize reach into the work area 31 The front grille shall be raised to prevent airflow blockage and improve comfort 32 Penetrations for petcocks and service fittings shall be provided penetrations shall be offset to improve user access 33 The cabinet shall accommodate an optional mounting stand for fixed height or adjustable height configurations 34 The sash window shall be able to be lowered further down by removing the arm rest to easily clean the upper back side of the glass Control and Alarm System 35 All cabinet functions shall be managed by a programmable microprocessor control system capable of software updates via Internet downloads 36 The microprocessor controller shall be mounted on the main control panel facing down toward the user 37 The controller shall include soft touch keypad controls and backlit LCD displays to permit operation of the blower motor light UV lamp electrical outlet s and menu 38 The LCD shall simultaneously display inflow downflow sash status airflow status and filter life 39 The controller shall be user programmable in situ to enable or disable functions such as PIN personal identification number access restriction cabinet start up protocol airflow alarm and other microprocessor controlled operations outlined in the user manual 40 When programmed ON the sta
20. l also be performed on a statistical sampling basis 4 Each cabinet shall be listed by Underwriters Laboratories UL CUL or CE for electrical safety 5 Original documentation specific to each cabinet serial number shall be provided with the cabinet and maintained in the manufacturers records Test data verifying all performance criteria shall be available upon request to include a inflow velocity through direct inflow measurement method b downflow velocity and uniformity c filter leak scan with aerosol challenge for both filters d light noise vibration e and electrical safety Filtration System 6 Thecabinet shall have one supply downflow filter and one exhaust filter Both filters shall be ULPA type per IEST RP CC001 3 and meet EN1822 H14 requirements 7 The filters shall be within an aluminum frame with mini pleat design without aluminum separators no wood or fiberboard shall be used in the filter assembly 8 Typical filter efficiency shall be gt 99 999 at MPPS and between 0 1 to 0 3 microns 9 Anintegral filter guard shall be affixed to prevent damage to the filter media The exhaust filter shall further be protected by angled staggered exhaust damper 10 The filters shall be a individually scan tested by the manufacturer b individually scan tested after assembly and b easily accessible for scan testing in situ by means of a dedicated upstream sampling port accessible from within the
21. lift and remove to provide easy access and encourage surface decontamination A recessed central area and stainless steel drain pan channel spills and External plumbing is concealed behind trim panels to maintain aesthetics External surfaces are coated with Esco Isocide antimicrobial coating to protect against surface contamination and inhibit bacterial growth Isocide eliminates 99 9 of surface bacteria within 24 hours of exposure Robust Cabinet Construction and Enhanced Safety Features Service fixtures are offset for easier reach Standard cabinets include two fixture provisions on each sidewall one provision on each sidewall for 0 9 meter 3 ft cabinet Electrical outlets are mounted below service fixtures to minimize obstructions Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated gas spring struts providing front service access All key components with the exception of the blower motor assembly are mounted outside the air stream and away from contaminated air to permit service without decontamination These include fluorescent lamps UV lamp electrical harnesses electronic boards and microprocessor control Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color coded red to warn service technician The telescoping Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when
22. maintains operator protection by discouraging users from placing objects on it Auto purge holes located at the front side walls eliminate eddy currents and dead air pockets in the critical area behind the sash window Per NSF ANSI 49 requirement these side perforations do not extend completely to the work surface to prevent accidental liquid spills in the work zone from entering the side air column Esco cabinets use Swedish Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the chance of leakage and to prolong filter life Filters include a lightweight aluminum frame for structural stability and elimination of swelling common to conventional wood frames Esco ULPA Filter Efficiency 96 Typical Penetration Modern separator less mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction The filter frame and media is constructed in accordance with EN1822 requirements for fire retardant properties The supply ULPA filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection The exhaust ULPA filter traps biohazard particles acquired from the work surface before air is exhausted to the room offering personnel and environmental protection Th
23. ositioned away from resistant PVC materials operator line of sight for safety and Adjustable height 395 490 mm proper exposure to interior surfaces 15 67 19 37 Note UV lamp intensity reduces over e Ergonomic foot rest time and its effectiveness is subject to Angled helps maintain proper factors such as relative humidity in the cabinet ambient air temperature and microbial species in the work zone e PVC armrest Chemically treated improves operator comfort easy to clean 712 mm e Electrical outlet Europe Worldwide e Petcock air gas vacuum Labculture Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets General Specifications International Models LA2 3A1 LA2 4A1 LA2 5A1 LA2 6A1 Europe Asia Pacific Africa LA2 3A3 LA2 4A3 LA2 5A3 LA2 6A3 Latin America 230 V 50 amp 60 Hz Nominal Size 0 9 meters 3 1 2 meters 4 1 5 meters 5 1 8 meters 6 External Dimensions 1115 x 852 x 1540 mm 1420 x 852 x 1540 mm 1725 x 852 x 1540 mm 2030 x 852 x 1540 mm W x D x H 43 9 x 33 5 x 60 65 55 9 X33 XOGO 67 9 x 33 5 x 60 6 79 9 x 33 5 x 60 6 Gross Internal Dimensions 970 x 623 x 670 mm 1270 x 623 x 670 mm 1570 x 623 x 670 mm 1870 x 623 x 670 mm WxDxH 38 2 x 24 5 x 26 4 50 0 x 24 5 x 26 4 61 8 x 24 5 x 26 4 73 6 x 24 5 x 26 4 Usable Work Area 0 45 m 4 8 sq ft 0 6 m 6 5 sq ft 0 75 m 8 1 sq ft 0 9 m 9 7 sq ft Tes
24. ramming capabilities built into the Sentinel microprocessor platform The field calibration mode makes it easier for certifiers to calibrate the airflow display RS 232 data output port enables remote monitoring of cabinet operating parameters Optional TCP IP converter to connect RS MICROPROCESSD When programmed ON e the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities Cabinet Construction Robust construction and enhanced safety features qualify the cabinet for the most e demanding laboratory applications The cabinet is fully assembled and ready to install and operate when shipped prevent liquids from entering the lower filtration and blower systems The drain pan is flush with the side walls to eliminate concealed or hard to clean spaces e There are no screws in the front or sides to trap contaminants or complicate cleaning e The interior work area is formed from a single piece of stainless steel with large radius corners to simplify cleaning e Optional service fittings are offset for 5 e The cabinet work zone has no welded easier access 232 to network for remote monitoring RS 232 SERIAL joints to collect contaminants or rust All stainless steel work surfaces are accessible for cleaning Tray components
25. re the cabinet for normal operation and advise when safe conditions are established e Selectable Quickstart mode to automatically turn on off the cabinet blower and lights by simply moving the sash window to the correct position e An administrator controlled PIN Personal Identification Number can be set to restrict access to Main menu e The airflow alarm can be activated or deactivated depending on user preference and nature of the work Sentinel M Gold Microprocessor Control System Programmable Touchpad data entry buttons permit control settings and access to diagnostics default A graphical interface indicates cabinet performance Digital read out with alpha numeric display indicates all input status and alarm functions All functions can be user activated through touch pad programming access see Operations Manual Ecco Color coded indicator lamps display green for primary function settings and hierarchical Diagnostics button to fan operation blue Programmable menus easily check the cabinet for secondary function automatic UV light operating parameters and fluorescent lights and timer simplifies operation enhances contamination control extends UV lamp life and saves energy assist servicing electrical outlet and orange for caution UV lamp ON imiwimi iLicie E m Consult your Esco Operating Manual or contact your Sales Representative for information on user preference prog
26. regional and international criteria Testing in our microbiology laboratory is conducted according to NSF ANSI 49 EN 12469 and JIS K3800 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all testing work Harmless Bacillus Subtilis bacteria is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Units CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone Bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet workzone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B Subtilis spores is placed inside the workzone 10 cm 4 inches behind the front opening sash e Target slit air samplers and impingers are placed outside the workzone to capture possibly escaping B Subtilis spores then the sample is incubated e Acceptance The number of Bacillus Subtilis CFU recovered from the agar plates shall not exceed 10 CFU per test The Performance Envelope Concept 0 65 m s High Inflow High Downflow 0 60 0 61 0 43 High Inflow Low Downflow 0 58 0 27 0 55 Nominal 0 53 0 35 gt H
27. rotection e ULPA filters per IEST RP CC001 3 The Esco Labculture Class Il Type A2 Biological Safety Cabinet provides operator product and environmental protection against Biosafety Levels 1 2 and 3 This cabinet can be used for handling Biosafety Level 4 provided that the operator wears positive pressure suit Containment and Protection The airflow ratio of 6396 recirculation to 37 exhaust increases operator protection beyond the 7096 3096 ratio of conventional biological safety cabinets e The inflow and downflow balance is are tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron particles these provide better filtration capability than conventional HEPA filters that have a typical efficiency of 299 9996 for 0 3 micron particles Mini pleat Separatorless Filter left vs Conventional Aluminum Separator Filter right precisely established by an external exhaust damper and is adjustable without decontaminating the cabinet Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination The front grille has proportionally larger perforations on the extreme left and right side Combined with the side air gap between the work tray and the side wall the stronger side capture zones increase protection in this critical area where contaminants tend to bleed out in conventional cabinets The convex inflow grille
28. rt up protocol shall perform an automatic pre purge and post purge cycle to ensure proper cabinet operation 41 The controller shall include a blower motor hours meter to display aggregate motor running time to assist in predictive maintenance 42 Audible and visual alarms shall be provided for unsafe conditions such as improper airflow or sash position 43 Airflow shall be monitored by a temperature compensating thermistor based true air velocity sensor mounted in the cabinet 44 The airflow display and alarm system shall be individually calibrated before shipment 45 The main control panel shall exhibit continuous display of air velocity and a 24 hour clock display 46 Diagnostics button should be available on the control panel to easily check the cabinet operating parameters and assist servicing 47 The cabinet shall have field calibration mode that simplifies on site calibration mode 48 A selectable Quickstart mode should be available to automatically turn the blower and lights on off by moving the sash window to correct position 49 The BSC shall have RS 232 data output port for remote monitoring of cabinet operating parameters 50 TCP IP converter shall be available as an option to connect RS 232 to network for remote monitoring Certification Service and Decontamination 51 The cabinet shall be approved for both hydrogen peroxide vapor HPV and formaldehyde decontamination protocol 52 All p
29. safe it locks the counter balance in place if either of the 2 cables is detached The sash cable and cable clip have rated strength of more than 6 times the weight of the sash window of the largest Labculture Class Il cabinet 1 8 meter 6 ft model Blower Efficiency The Labculture blower system is designed for high performance operation maximum energy efficiency and minimal maintenance e The external rotor motor design allows for optimum cooling of the motor during extended operations and extends the motor bearing life e The blower motor can maintain airflow for a 15096 increase in pressure loss over clean filters without manual speed control adjustment or 210 increase in pressure drop across the filter with manual speed control Cabinet Filtration System Side capture zones Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum blower plenum at the top of the cabinet Approximately 37 of the air in the common plenum is exhausted through the ULPA filter to the room The remaining 63 of the air is passed through the downflow ULPA filter and into the work area as a vertical laminar flow air stream bathing the
30. ted Opening 229 mm 9 229 mm 9 229 mm 9 203 mm 8 Working Opening 274 mm 10 8 274 mm 10 8 274 mm 10 8 248 mm 9 8 NEE Na low 0 53 m s 105 fpm Velocity Downflow 0 35 m s 70 fom 0 35 m s 70 fpm 0 35 m s 70 fpm 0 33 m s 65 fom Inflow 424 m h 251 cfm 555 m h 328 cfm 686 m h 406 cfm 724 m h 426 cfm Downflow 63 709 m3 h 424 cfm 928 m h 555 cfm 1147 m h 686 cfm 1289 m h 759 cfm Exhaust 3796 424 m h 251 cfm 555 m h 328 cfm 686 m h 406 cfm 724 m3 h 426 cfm Required Exhaust With Optional Thimble Exhaust 529 m h 311 cfm 764 m h 450 cfm 1116 m h 657 cfm 1164 m h 685 cfm Collar Airflow Volume Static Pressure For Optional Thimble Exhaust Collar 32 Pa 0 12 in H O 49 Pa 0 19 in HO 62 Pay 0 24 in H O 79 Pa 0 31 in H O ULPA Filter Typical Efficiency gt 99 999 at particle size between 0 1 to 0 3 microns NSF ANSI 49 lt 61 dBA lt 61 dBA lt 62 5 dBA lt 63 dBA Sound Emission EN 12469 lt 58 dBA lt 58 dBA lt 59 5 dBA lt 60 dBA E E a Res gt 1230 Lux gt 1400 Lux gt 1070 Lux gt 1225 Lux C p y gt 114 foot candles gt 130 foot candles gt 100 foot candles gt 114 foot candles Electrogalvanized steel with Isocide oven baked epoxy polyester powder coating Cabinet Construction 1 2 mm 0 05 18 gauge 220 240V AC 50Hz 1 LA2 3A1 LA2 4A1 LA2 5A1 LA2 6A1 Cabin
31. this page for the full list e Contact Esco or your Sales Representative for site preparation information see Electrical Specifications s E Antimicrobial Powder Coating 16 hours 20 hours 24 hours Contact Time in Hours All exterior painted surfaces are powder coated with Esco Isocide an antimicrobial inhibitor to minimize contamination Isocide is integrated into the coating substrate and cannot wash out or diminish by repeated cleaning Performance results are available upon request Labculture Biological Safety Cabinet e Class Il Type A2 Biological Safety Cabinets Warranty The Labculture cabinet is warranted for 3 years excluding consumable parts and accessories e Each cabinet is shipped with a compre hensive user s manual complete with a report documenting all test procedures e Additional IQ OQ documentation is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests Accessories and Options Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Represen tative for ordering information Support Stands e Fixed height available 711 mm 28 or 864 mm 34 x38 1 mm 1 5 With leveling feet With casters e Adjustable height hydraulic range 711 mm to 864 mm 28 to 34 With leveling feet With casters e Telescoping height stand for leveling feet nominal ran
32. tivity and reduces the time to seal the cabinet e HPV biological efficacy is independent of environmental variables whereas formaldehyde efficacy is dependent on such variables e HPV has a better penetration capacity resulting in a full decontamination of the cabinet The formaldehyde method is known to result in incomplete decontamination e HPV is more effective and rapid against biological organisms compared to formaldehyde e HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hours needed to complete a formaldehyde decontamination process e HPV decontamination effectiveness is independent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 6596 e For information on the BIOQUELL and STERIS HPV methodologies contact Esco or your Sales Representative and ask for our HPV Decontamination Whitepapers KI Discus Containment Test According to EN 12469 Potassium lodide Esco is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is defined in the European Standard for microbiological safety cabinets EN 12469 as a test method for validating the operator personnel protection capabilities of the cabinet e The KI Discus test shows excellent correlation with the microbiological test method for operator protection and is useful for
33. utility value and safety e The 10 angled viewing window and narrow profile front grille improves reach into the work area e The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy e The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Technical Specifications Bacterial Population 10 folds 8 hours 4 hours 12 hours e The front armrest is raised above the workzone to improve comfort and to ensure that the operator s arms do not block the forward airflow perforations e The optional adjustable support stand provides work surface height control e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equipment e The sliding window can be fully opened to insert and remove larger instrumentation and equipment Electrical Safety and Certification All components meet or exceed applicable safety requirements e Each cabinet is individually tested for electrical safety at factory e Documentation specific to each cabinet serial number is maintained on file e UL Listed for USA and Canada e Certified to all major world standards for biological safety cabinets including the NSF ANSI 49 and EN 12469 refer to Standards Compliance table on
34. validating the actual containment performance of the cabinet on site e The Kl Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus each Esco Labculture LA2 model is factory tested on a sampling basis using the KI Discus method for operator safety Purchase Specifications LA2 Series Class Il Type A2 Biological Safety Cabinet General Performance and Certifications 1 The biological safety cabinet shall comply with one or more of the following international standards and the manufacturer shall provide a certified copy of containment and performance tests equivalent to or greater than specified in the following independent international standards for biological safety electrical and other functional characteristics Class Il Type A2 per NSF ANSI 49 USA Class Il per EN 12469 JIS K3800 SFDA YY 0569 SANS 12469 2 The cabinet shall protect a the operator and laboratory environment from particulates generated within the work zone b the product and process within the work zone from airborne contamination from ambient air c and the product and process within the work zone from cross contamination 3 Thecabinetshall be tested by KI Discus test European Standard EN12469 2000 on statistical sampling basis to validate operator personnel protection The retention efficiency for the front aperture shall be not less than 99 999956 Microbiological testing for cabinet performance shal
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