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Getting started with Image Quant

1. Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 5 Performing an exposure 4 5 7 Save the image Step Action 2 Click the Save button or select Save in the File menu to save the image Result The Save dialog opens LAS4000lmage z e amp etES My Recent Documents Desktop My Documents My Computer My Network File name Places Save as type Gel Fie Foma vl 2 Choose a folder file name and file format then click the Save button Note gel format A file format developed by GE Healthcare Exposure details are maintained tiff format A 16 bit TIFF format suitable for analysis in external software Exposure details are not maintained 3 Click the Complete button Result The current display returns to the initial screen 4 4 After exposure If the ImageQuant LAS 4000 will not be used for some time it should be switched off as follows Step Operation Select Quit from the File menu I ImageQuant LAS 4000 EM Edit View Option Page Setup nt Quit T FTELISIUIT Getting Started with ImageQuant LAS 4000 28 9607 59 AB 59 4 Operation 4 4 After exposure Step Operation Select Stop the CCD cooling now Click the OK button Stop the CCD cooling now Keep the CCD cooling after quit 2 OK Cancel TIP If Keep the CCD cooling after quit is selected the cooling temperature of the CCD will
2. 575DF20 Cy3 filter for green LED 605DF40 EtBr filter for detecting EtBr 510DF10 filter for detecting GFP Y515 filter for blue LED L41 UV filter for UV LED 7 Sample tray Epi tray for chemiluminescence and Epi lights White trans tray for white light table UV trans tray for UV transilluminator NP tray for titer plates 8 Power switch Turn the ImageQuant LAS 4000 on or off Computer The ImageQuant LAS 4000 is operated using a PC running Windows XP or VISTA It is not designed for standalone operation Note The computer used should be certified according to IEC UL 60950 1 4 ImageQuant LAS 4000 Control Software Exposure and exposure settings are controlled by the ImageQuant LAS 4000 Control Software The software user interface is described below 10 Getting Started with ImageQuant LAS 4000 28 9607 59 AB Fle Edt View Option Help ImageQuant LAS 4000 Exposure Type ren v 1 Introduction 1 4 ImageQuant LAS 4000 Control Softwa re Part Name Description 1 Menu bar Access to program functions 2 Exposure Type Set the exposure method 3 Exposure Time Set the exposure time 4 Sensitivity Resolution Set the sensitivity and resolution 5 Add Digitization Image Add a white light exposure to a chemiluminescent exposure used for overlaying a marker image 6 Setting state CCD The state of the CCD and exposure type
3. Remove the foreign object The locking section is damaged Contactyour GE Healthcare represen tative The initial diagnosis does not start even if the power is turned on The camera cable is not connected correctly Turn the power off and check the camera cable connection A focused image is not displayed Power cable and cam era cable connection failure Connect the AC power cable and camera cable correctly Connector connection failure Light failure Connect the trans light source by pushing it all the way until it hits the other end Lock the Epi lights in place Foreign object is present in the door sensor Remove the foreign object Software is not in the Focusing mode Click the Focusing button in the soft ware Getting Started with ImageQuant LAS 4000 28 9607 59 AB 67 6 Troubleshooting 6 5 Problems with ImageQuant LAS 4000 Phenomenon Countermeasure The light source is lit even when Interlock failure the ImageQuant LAS 4000 door is opened Turn off the power immediately and contact your GE Healthcare represen tative 6 4 Problems with ImageQuant LAS 4000 Control Software If the error persists even after the following countermeasures have been taken fill in the necessary items and the error code in the Service report fax sheet at the end of the User Manual Phenomenon ImageQuant LAS 4000 Control Soft ware cannot recognize ImageQuant
4. 7 Setting state IDX Current settings of tray position and illumination inside the dark box 8 Method Tray position Set the detection method and tray position 9 Focusing Adjust the focus 10 Start Start the exposure Getting Started with ImageQuant LAS 4000 28 9607 59 AB 11 2 Safety instructions 2 Safety instructions This chapter describes safety precautions safety labels and emergency procedures for the ImageQuant LAS 4000 in addition to information on the safe disposal of the instrument 2 1 Safety precautions Introduction The ImageQuant LAS 4000 is powered by mains voltage and is used to image samples that may be hazardous Before installing operating or maintaining the equipment you must be aware of the hazards described in the user documentation Follow the instructions provided to avoid personal injury or damage to the equipment General precautions WARNING Do not use the equipment if smoke strange noises or strange odors can be perceived or if the equipment becomes unusually hot This may result in fire or electric shock Stop using immediately turn off the power switch and unplug the equipment from the power outlet Contact your local GE Healthcare representative to request repair WARNING Do not allow liquids flammable materials or metallic objects to get into the camera head or the ImageQuant LAS 4000 This may result in fire or electric shock Turn off the power switch unp
5. c Place the sample on the gel sheet Sample Gel sheet Note The gel sheet can be used repeatedly After use wash it with mild detergent rinse with water then dry well A gel sheet can be reused about 20 times 50 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 2 Preparation 4 2 3 Placing the sample 3 Choose the exposure size and tray position For Epi and Trans trays e The readable area varies depending on the tray position Place the sample in position according to its size 250mm x 250mm 210mm X 140mm 180mm X 120mm 144mm X 96mm 105mm X 70mm Position 1 Position 4 Maximum capture size when a wide view lens is used TIP On the Epi tray there are round dents marked on the tray for positioning the sample Line up the sample using the appropriate dents For NP tray The NP tray is to be used at tray position 3 Note The readable area will be slightly smaller using the F1 8 wide view lens Getting Started with ImageQuant LAS 4000 28 9607 59 AB 51 4 Operation 4 2 Preparation 4 2 3 Placing the sample 4 Open the IDX door and insert the tray Place the Epi or Trans trays in position with the side with a hole facing outwards 5 Ensure the lens cap is removed and close the IDX door 4 5 Performing an exposure This section describes the steps for exposing a sample and saving the image In this description the screen images shown are for exposure of an ethidium bromide EtBr s
6. 35 3 5 3 Installing the lens een EU n Pape isum IUCUNDE NM ARES oe Names 35 354 Attaching eder to The TUEer changen acest etasatid tane as quita tac nets OEE 37 3 5 5 cus ve Fekcen san ecole ia NC 39 35 6 Inst lling EDO SOUTE S MM RR iieiea e aiee EEEE EEEE EE EA EERE a 40 fi erige 42 4 1 Operation Overview RM 42 a2 Prepar OW RR m 42 4 2 1 Starting the ImageQuant LAS 4000 and Control Software eoe enn WT TU RRTERRRUARE 42 4 2 2 Preparing the ImageQuant LAS 4000 for exposure Guess exisse d sime epis tit bt e das mU RdE 43 S83 PIOS TNE SOTIDIE user ape PATRE iseni ERO ARS DUAE vH ER E OD EI 49 4 3 Performing AN sspOSUle uude ied it e de aUe AXH HE REn S inerte Lr NE 52 45 MRE UO os MEME ee eee 5g 4 3 2 Choose method and tray position ossia ven a tite dndisaui tine in n rt Oba sca Odit E AN dn 55 v 8 51 2 ir 0o Tr C n 55 4 3 4 Set exposure type and exposure Te issues emt tes E NET USt E uia GER I Qa 55 35 Ser MOS IE SEDI VIDI rionn HR AREE N LU IU ER RTI AUR A 56 2 56 SEO SU Cee cscebsUEDUERUDUEUIMEUDME A IHE I EEUU LIE EMI MN NDEEN 57 437 Save TA An 58 2 SABOISSDOSMIS pete nee eme ee cee eee renee uade nea 59 5 1 ire MR 61 51 ImageoudntbAS A000 eto ede CHI E E OR HER tH HEURES 61 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Table of Contents 6
7. 43mm LAS High Sens lens F1 8 24mm wide view lens For instructions on how to change the lens refer to the ImageQuant LAS 4000 User Manual Getting Started with ImageQuant LAS 4000 28 9607 59 AB 43 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 for exposure Changing the light sources 44 Both trans transmitted and epi incident light sources are available for exposure with the ImageQuant LAS 4000 Type Name Peak wavelength UV transilluminator 312 nm Transmitted White light table UV Epi light 365 nm Blue Epi light 460 nm Green Epi light 520 nm Incident Red Epi light 630 nm NIR Epi light 710nm White Epi light Changing the transmitted light source 1 Ifnotransmitted light source is currently in place inside the instrument proceed to step 4 2 Turn the lever holding the transmitted light source to unlock it 3 Pullout the transmitted light source carefully along the guide while holding it with both hands 4 Pushthe new transmitted light source in along the guide at the bottom of the IDX Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 for exposure 5 Turn the lever at the front to lock the light source into position Changing the Epi lights This section describes how to change a set of Epi lights Note The tray should be at tray position 4 before installin
8. Filename starts with an in valid character Please input a filename The filename is blank Supply a valid file name The wait time or exposure time of image xx is out of range Please change The wait time or exposure time is not appropriate for the specified image Change the wait and exposure times to appropriate values Method Tray Position button Error message Cannot identlify the light source Please check that the light unit is in stalled correctly Meaning The light source could not be identified Countermeasure Ensure that the light source is installed correctly Cannot find the appropriate filter for the method Please install an appro priate filter The appropriate filter is not in place Make sure the correct filter for the method is in place and registered in the software Option menu FlatFrame Calibration Error message The door was opened during expo sure Flat frame calibration failed Meaning The door was opened dur ing flat frame calibration Countermeasure Close the door again and per form the flat frame calibration again An error occurred during flat frame calibration for Cy3 tray position 4 Please check that the correct tray and calibration plate were used Flat Frame calibration im age exposure failed Check that the correct sample tray and calibration plate are in place and re calibrate Get
9. LAS 4000 Cause USB cable connection failure Countermeasure Connect the USB cable correctly to the camera head and PC Power cable or camera cable connection failure Connect the power cable and camera ca ble correctly The CCD temperature indication does not change to READY or its value does not approach the set temperature af tera sufficient time about 15 minutes has elapsed since the start of cooling The ambient temperature is too high Set the ambient temperature to 28 C or less The CCD temperature is set too low Set the cooling temperature of the CCD properly USB cable connection failure Connect the USB cable correctly The camera head ventilation holes are blocked Confirm that nothing is blocking the venti lation holes No focus image is displayed in the Fo cusing mode USB cable connection failure Connect the USB cable correctly Brightness of reading soft ware is too high or low Adjust the Brightness in the reading soft ware The lens is not correctly in stalled Install the lens correctly The iris is not correct Check the iris value The image is noticeably out of focus Adjust the focus Even when the set exposure time has elapsed the reading operation is not completed The ImageQuant LAS 4000 power has been turned off during exposure Turn the power switch of the ImageQuant LAS 4000 on and reboot it 68
10. Mar Program Getting Started with ImageQuant LAS 4000 28 9607 59 AB 55 4 Operation 4 5 Performing an exposure 4 3 4 Set exposure type and exposure time Step Action 2 Select Auto or Manual for Exposure Time Auto Exposure Type Exposure Time Auto Note O Manual the sample type and method e Manual Automatic exposure time setting may not be possible depending on Select an exposure time from the drop down list or enter the exposure time manually The exposure time can be set from 0 01 seconds up to 30 hours Exposure Type Add Digitiz ON Precision v Exposure Time O Auto Manual i Sensitivity 4 3 5 Set exposure sensitivity The sensitivity can be improved by binning multiple pixels into one larger pixel Smoothing by interpolation then increases the number of pixels for the final image The sensitivity increase in order Standard High Super and Ultra Sensitivity No pixels binned WxH No pixels final image W x H High Resolution 3072 x 2048 3072 x 2048 Standard 1536 x 1024 1536 x 1024 High 768x512 1536 x 1024 Super 384 x 256 1536 x 1024 Ultra 192 x 128 1536 x 1024 High Binning 768 X 512 768 x 512 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 5 Performing an exposure 4 3 5 Set exposure sensitivity Sensitivity No pixels binned WxH No pixels final image W x H Super
11. aging allergic reactions and cancer Medication or cosmetics may increase sensitivity to UV radiation Consult a physician before using this product if using medication if you have known skin disorders or if you are especially sensitive to sunlight Excessive exposure of the eyes can result in permanent damage to your vision CAUTION Do not stare at the light emitted from the LED lights sources This may impair vision Getting Started with ImageQuant LAS 4000 28 9607 59 AB 13 2 Safety instructions 2 1 Safety precautions Operation WARNING Do not use the equipment with a power supply other than that recommended Fire and electric shock could result WARNING Do not use the equipment within or near a sink or in humid or dusty environments Fire and electric shock could result WARNING If thunder can be heard do not touch the power supply plug or electric shock could result WARNING Connect the power supply directly to a grounded wall power outlet The use of extension cords or multiple loads on one electrical outlet could result in fire and electric shock CAUTION Do not use the same power supply as that of large equipment such as an air conditioner or centrifuge Malfunction could result CAUTION Do not block the vents and ensure that the vents are kept free of dust and dirt Blockage of the vents can result in overheating of the equipment and malfunction P
12. be connected with a USB cable during the operation Open the control panel and select Printers and Other Hardware Click Add Hardware Click the Next button in the Add Hardware Wizard Select Yes have already connected the hardware and click the Next button CU f WNW N e Select Add a new hardware device and click the Next button Add Hardware Wizard The following hardware is already installed on your computer From the list below select an installed hardware device then click Next to check properties or troubleshoot a problem you might be having To add hardware not shown in the list click Add a new hardware device Installed hardware USB Root Hub C USB Mass Storage Device Generic USB Hub USB Composite Device S Generic volume x lt Back Next gt Cancel 6 Select Install the hardware that I manually select from a list Advanced and click the Next button Getting Started with ImageQuant LAS 4000 28 9607 59 AB 27 3 Installation 3 4 Installation of the software 3 4 1 Installation Windows XP 7 Select Show All Devices and click the Next button Add Hardware Wizard From the list below select the type of hardware you are installing eS If you do not see the hardware category you want click Show All Devices Common hardware types Shi es 8 Display adapters Fujifilm USB Device EIDE ATA ATAPI controllers amp IEEE 1394 Bus host controllers SB Imaging devices A Infrared device
13. be maintained and you can immediately start using it upon simply restarting Image Quant LAS 4000 Control Software This is useful if more samples will be exposed later in the day 3 Switch off the computer 4 Turn off the ImageQuant LAS 4000 power switch 60 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 5 Maintenance 5 Maintenance To ensure optimal performance the ImageQuant LAS 4000 and accessories should be maintained regularly This chapter describes the maintenance that should be performed on a regular basis NOTICE eo Before performing any maintenance on or inside the ImageQuant LAS 4000 be sure to turn off the power switch and remove the power plug from the electrical outlet 5 1 ImageQuant LAS 4000 Use a soft dry cloth to clean the exterior of the ImageQuant LAS 4000 For marks that are hard to remove wipe with a soft cloth slightly dampened with a neutral detergent then wipe with a new clean dry cloth To clean the interior of the instrument use a non fluorescent cleanser for biochemical use If using a neutral domestic detergent the detergent residue may fluoresce when exposing images NOTICE eo Do not use organic solvents These may damage the surface 5 2 Sample trays After using a sample tray wash it in water using a soft sponge dipped in a neutral detergent After cleaning leave the tray to air dry WARNING Wear gloves while cleaning There may be residues
14. contains in valid characters the invalid characters Change the filename excluding Error message The environmental temperature is too high to cool the CCD Meaning The ambient temperature is too high for the CCD to cool Countermeasure Turn off the the ImageQuant LAS 4000 reduce the air tem perature around the instru ment and then restart Image Quant LAS 4000 and Image Quant LAS 4000 Control Soft ware Getting Started with ImageQuant LAS 4000 28 9607 59 AB 6 Troubleshooting 6 6 Error messages and countermeasures Error message Meaning Countermeasure Components in ImageQuant LAS The default settings have Change the method and or the 4000 have changed been changed tray position in the Please change the exposure method Method Tray Position dialog Please change settings in Method Tray Position window Start button starting exposure Error message Meaning Countermeasure The interval time must be greater The time value is inappro Enter an appropriate time value that 10 seconds when using the In priate crement Repetition mode The maximum exposure time when using the EtBr method is 10 minutes The maximum exposure time when using the UV light is 10 minutes The maximum interval time is 2 hours The maximum exposure time is 30 hours The maximum interval time when using the EtBr method is 60 seconds The maximum interval t
15. do not handle the connection plugs with wet hands CAUTION Connect the computer hardware on the same power circuit otherwise the equipment may be influenced by electrical nosie CAUTION Turn off the power switch and remove connecting cables before moving the equipment CAUTION Turn the power switch off before cleaning the inside of the equipment CAUTION Unplug the equipment if it will not be used for an extended period gt gt gt Getting Started with ImageQuant LAS 4000 28 9607 59 AB 17 2 Safety instructions 2 2 Labels 2 2 Labels On the exterior of the ImageQuant LAS 4000 there are a number of labels describing equipment specifications as well as precautions necessary to safely use the ImageQuant LAS 4000 This section lists the symbols used on the safety labels and shows the location of labels on each component The names of parts on the components are also shown ImageQuant LAS 4000 serial number The ImageQuant LAS 4000 serial number is located on a label on the back of the instrument care Bio Sciences AB LUMINESCENT IMAGE ANALYZER Model ImageQuant LAS 4000 SerialNo 123456 50 60 Hz Supply Voltage 100 240 V us rren 3 0A 1 5A LISTED Protection Class IP20 LABORATORY EQUIPMENT Manufacturing Year Maniciw FUJIFILM Corporation TOKYO 106 4620 JAPAN MADE IN JAPAN 405N101506 Symbols used in safety labels Label Meaning Warning
16. errors see Section 6 5 Message ImageQuant LAS 4000 hardware error occurred on page 69 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 71 6 Troubleshooting 6 6 Error messages and countermeasures General error messages Start up 72 Error message The hard disk is full Cannot save Disk capacity is insuffi cient Exposure failed Disk working space is insufficient Meaning Disk space is insufficient Countermeasure Free some disk space Cannot save File I O Error An error occurred during writing the file Check the file name and folder are correct Check that the PC is operating correctly Cannot save The folder is write pro tected The folder is write protected Please change the folder The folder to which the file is to be written is write protected Save to a different folder or cancel the write protection Cannot save A file with the same name is write protected A file with the same name is write protected the file or save to a file with a Cancel the write protection of different name File open error An error occurred while opening the file Check the correct file is chosen Check the PC is operating cor rectly In the worst case the file may have become corrupted Please close the door The IDX door is open Close the door Can not use the characters below for the filename lt gt The filename
17. of hazardous chemicals NOTICE o Do not use organic solvents These may damage the surface Getting Started with ImageQuant LAS 4000 28 9607 59 AB 61 5 Maintenance 5 2 Sample trays NOTICE o Do not use abrasive cleaning materials such as a scouring pad for cleaning This may scratch the surface 5 5 Lens filters NP tray and Epi light sources Use a soft dry cloth only to clean these components To maintain image quality when cleaning optical components the following points should be observed Never touch the optics lens camera head window filter surfaces or Epi light windows with bare hands Use an air blower to remove dirt or dust If the optics have been touched accidentally wipe the soiled surface using lens cleaning paper dipped in lens cleaner solution Any grease allowed to remain on the surface may become difficult to remove cause degeneration and possibly molding Do not adjust fixing screws or tie rings on the components unnecessarily This may cause the performance to deteriorate Use the protective caps on the camera head and lens whenever they are not installed in the ImageQuant LAS 4000 Store these protective caps carefully Do not allow dirt or dust to accumulate on the inlet and outlet vents of the camera head Use a soft dry cloth to clean them 5 4 UV transilluminator and white light table Use an air blower to remove dust Greasy residues can be cleaned with a soft c
18. 03 Class A EN ISO 12100 1 12100 2 Safety of machinery Basic concepts Harmonized with general principles for design 2006 42 EC Getting Started with ImageQuant LAS 4000 28 9607 59 AB N 1 Introduction 1 2 Regulatory information Standard Description EN ISO 14121 1 14121 2 Safety of machinery Principles of risk Harmonized with assessment 2006 42 EC CE Marking The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in the user documentation and used in the same state as it was delivered from GE Healthcare except for alterations described in the user documentation Regulatory compliance of connected equipment A 6 C ny equipment connected to the ProductNameHW should meet the safety requirements of EN 1010 1 IEC 61010 1 or relevant harmonized standards Within EU connected equipment must be E marked 1 3 The mageQuant LAS 4000 Feature overview The following features are offered by the ImageQuant LAS 4000 Digitized images of stained gels membranes or films can be obtained by white light illumination A super CCD camera of 3 2 megapixels is used An effective resolution of 6 3 megapixels can be obtained by the image processing procedure Internal cooling of the CCD contributes to a low t
19. 140 White light table error Bl Errors B104 to B120 above except described Qe generated in combina above tion A000 Communication error be Restart the ImageQuant LAS 4000 and Image tween camera head and Im Quant LAS 4000 Control Software ageQuant LAS 4000 4 A100 Communication retry error between camera head and ImageQuant LAS 4000 A200 70 Getting Started with ImageQuant LAS 4000 28 9607 59 AB SensKey 6 Troubleshooting 6 5 Message ImageQuant LAS 4000 hardware error occurred Countermeasure SensCode Content Communication parameter error between camera head and ImageQuant LAS 4000 DOOO Memory error during startup diagnosis D100 Image data memory error during startup diagnosis D2 Shutter error during startup diagnosis D300 Time out error of startup diag nosis D400 Time out error of filter or iris operations D500 Time out error of lifting table operation D600 Interrupt processing error D700 LED connection error D800 Board fan error D900 Camera head fan error DCOO Shutter error during exposure E000 OS system call error 6 6 Error messages and countermeasures The tables below describe error messages that can foreseeably be generated by the software These are categorized according to the operation that can cause the error along with an explanation of the error and an appropriate countermeasure For a description of hardware
20. 365 nm Fluorescence DAPI Fluorescence using Blue Epi light 460 nm Fluorescence Cy2 GFP Fluorescence using Green Epi light 520 nm Fluorescence Cy3 Fluorescence using Red Epi light 630 nm Fluorescence Cy5 Fluorescence using NIR Epi light 710 nm Fluorescence DAPI Sample digitization using White Epi light Digitization Epi illumination Sample digitization using White light table Digitization Trans illumination 3 Select Tray position according to the sample size or type E 105x 70mm 1 O2 l2 Os NP EN O4 4 Click the OK button 54 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 5 Performing an exposure 4 3 3 Focus adjustment 4 3 3 Focus adjustment Step Action 1 Click the Focusing button Result The focusing controls appear TUAM Vos Function 1 Decrease image brightness 2 Increase image brightness Coarse focus adjustment 4 Fine focus adjustment 5 Return to the main display 6 Click on the image to zoom Note Adjusting the brightness does not influence the actual exposure 2 Check that the sample is correctly positioned and focus the image 3 Click the Return button 4 3 4 Set exposure type and exposure time For a detailed description of the different exposure types refer to the ImageQuant LAS 4000 User Manudl Step Action 1 Select the Exposure Type from the drop down list Exposure Type Pi w Precision Exposure mE mI Oo Auto Repetition
21. 52 SONIS SIDE een ae ec nnn S eund Eai ean ie Anan ana sE Md 61 5 3 Lens filters NP tray and Epi light Sources osos CUIU n exe e oed Ch dae arae qvae ltitit e 62 5 4 UV transilluminator and white light table uscite vemertte e pest rorute pietate raten i erret 62 JEN o B18 2161112 MOMENT 62 TEONDIGSDEDUIBd acide D et ee la Ern LU D M M E 64 6 1 Problems with the exposed iMage conati on divo int Gel onion dtt icon tix Rn aie ep Even 65 6 2 Strange sounds or odors dre perceived asassssuxescistnsituteratenstsenit iem tom dete ver IIS HH UP I GM 66 6 3 Problems with ImageQuant LAS 4000 git d iH RH aves ERU GU ER OS 67 6 4 Problems with ImageQuant LAS 4000 Control Software esent 68 6 5 Message ImageQuant LAS 4000 hardware error occurred sstteeenntttttnnnnn 69 6 6 Error messages and countermeasUres ssesssssssssesseccssssssesssececcsessussssstsesesceccesssnseeseceeeeessunmmestesseseseneessnseeseseeeees 71 Reference INIDEIOURD Pacta cil philos tei E ie ako aria einai ieee omnia 79 7 1 Main Specifications sacas ditte P RSRT IRE KU ERE DU md tU uitdsq eres dente tus uvis 79 Te MINIT computer requirements osadeuong RHODE RUD OU UTEM SU ENDURO fad GRE nians 84 Zr Uterat re Los quERd CRM EM EIU Ren E M AME MM M MEM MM EM EM 84 7 4 Ordering iINfOrmation oes cac eoe deb futu s btt dub eR Ted eterna 84 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 1 Introduction 1 Introduction Purpose of this man
22. AS High Sens lens For instructions on installing the wide view lens refer to the ImageQuant LAS 4000 User Manudl 1 Ensure that the ImageQuant LAS 4000 and the PC are connected Turn on the instrument and the PC and start the ImageQuant LAS 4000 Control Software 2 Select Lens Exchange in the Option menu LAS 4000 fem Help CCD Calibration FlatFrame Calibration FlatFrame Calibration Pro Method Setting A message is displayed Close the door and click OK button ImageQuant LAS 4000 will make ready to shut down Click OK Getting Started with ImageQuant LAS 4000 28 9607 59 AB 35 3 Installation 3 5 Installation of hardware 3 5 3 Installing the lens 5 Bars Turn off ImageQuant LAS 4000 and change lens unit Turn off the instrument using the power switch 4 Remove the end cap from the lens 5 Insert the lens along the lens guide with the red point facing towards you Red points 7 Connect the lens cable Turn the knurling ring until it is securely fixed Knurling ring LN Connector of the lens cable 4 Note Be sure to connect the lens cable Otherwise the high sensitivity lens cannot be recognized This may also cause malfunction 36 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Installation 3 5 Installation of hardware 3 5 3 Installing the lens 8 Remove the lens cap Lens cap 9 Install the lens hood omm Lens ho
23. AUTION Do not repeatedly turn the power switch on and off unnecessarily This may cause malfunction CAUTION Do not leave samples in the equipment after exposure If left these may degrade and cause damage CAUTION The Epi lights are in sets of two for the left and right sides Ensure that they are correctly installed Do not mix light sources of differing types CAUTION Ensure that the Epi lights are connected and locked in position before turning the power on CAUTION Keep the windows of the light sources clean and avoid touching them NOTICE The UV trans tray will deteriorate with repeated UV radiation This is normal Replace the UV trans tray when this deterioration becomes noticable in exposed images 00D P PP Pe NOTICE Do not disconnect the USB cable while the Control Software is running Getting Started with ImageQuant LAS 4000 28 9607 59 AB 2 Safety instructions 2 1 Safety precautions Maintenance WARNING Do not remove the covers of the camera head or the ImageQuant LAS 4000 High temperature and high voltage parts could result in burns or electric shock Contact your local GE Healthcare representative to request internal inspection servicing and repairs WARNING Do not attempt to modify the equipment or fire and electric shock could result CAUTION Take care when connecting the power supply cable Do not tug on the cable and
24. Attaching a filter to the filter changer 6 Turn the filter changer put a filter in the desired position and secure it in place Change filter and Filter position Current Filters Select Filters 1 Through Through Y 2 Through i v Through 3 Through 605DF40 Y515 Di 4 Through S10DF10 5 Through il TRIS R670 575DF20 l OK l Filter down in Note The filter changer returns to the original position when you click the Start Focusing or Method Tray position buttons 3 5 5 Installing a transmitted light source This section describes how to insert the UV transilluminator or the white light table into the IDX 1 Push the transmitted light source along the guide at the bottom of the IDX Getting Started with ImageQuant LAS 4000 28 9607 59 AB 39 3 Installation 3 5 Installation of hardware 3 5 5 Installing a transmitted light source 2 Turn the lever at the front to lock the light source into position 3 5 6 Installing Epi light sources This section describes how to install a set of Epi lights Note The tray should be at tray position 4 before installing Epi lights If the tray is higher then it should be lowered to tray position 4 using the ImageQuant LAS 4000 Control Software before attaching the lights as described below Latch Epi light base 1 Ifthe tray is at Tray position 4 proceed to step 3 Otherwise close the IDX door 2 Click on Method Tray position set Tray posit
25. Binning 384 x 256 384 x 256 Ultra Binning 192 x 128 192 x 128 Step Action 1 Select Sensitivity from the drop down list Sensitivity Resolution High Resolution High Binning r Super Binning E RE Ultra Binning rc Note TIP 4 3 6 Exposure Step The Add Digitization Image function can be used for chemiluminescent samples This creates a composite image of a chemiluminescence exposure and a digitized white light exposure in order to add an image of an opaque molecular marker with a single click Select Sensitivity Resolution in the Help menu This displays a diagram that describes the relation between sensitivity and resolution Action 1 Click the Start button to begin exposure fie 6 xen eb ImageQuant AS DXX Note The orange Busy LED lights on the ImageQuant LAS 4000 during exposure Getting Started with ImageQuant LAS 4000 28 9607 59 AB 57 4 Operation 4 3 Performing an exposure 4 3 7 Save the image 4 3 7 Save the image Once the sample has been exposed the image will be loaded into the main window Step Action 1 Adjust the saturation and contrast of the image using the controls at the bottom of the window 1 m 2 Part Function 1 Adjust the dynamic range by dragging the limits with the mouse 2 The gradation conversion curve can be toggled between Linear or Sigmoid 3 The gradation can be altered by dragging with the mouse
26. GE Healthcare Getting Started with ImageQuant LAS 4000 Original instructions Table of Contents Table of Contents ME pers MRR 5 Li Important user InfanitlOD soeur tesa aere ADER UN ISEE HI SEASIVHEPEP IU PEE PeYE UR eT EesH SHE tr ec Uni E 5 l2 Regulatory snas Pr 7 1 3 helndgebudm LAS 4000 asc cautio tmb emcee acon UFU SI UNUM UM dE 8 1 4 ImageQuant LAS 4000 Control SOPIWOIB s acia mr n Vibo er albe ia RU ts rbvert ec tt Er 10 EC zs A estie ILES eere 12 21 SUD DESC MONS ocean rEPR ECHO CADENA I MEME 12 22 So 0 S eaa Re a REDE IRE E 18 23 Emergency ProcedUTE sniene E N E 24 24 FREY CUNT M OnOtI N az sasscesesSazazassvacteverngosssassssaysennasaenavasazascesovevsssvo ses aneegantasnasasasinsesesonsssaneranssecsnssssanenscesteesnuenniieeenat 24 S USO RR ec eco ee ene 26 ah SE TOQUE IIENIG cima aP HERES PEERISHERHU BI HURTS TURTUROISHRIU REI LE EHOR D GU UNDE UMEN 26 EMI 26 33 UDDIBOE IB scsuir oo eb peU e UD ee rt ana RU OD ERU HN O DU MPO NUUINU PUO RR RECHT a ai 26 3 4 Installation of the software opis RUM RRR Pen MPH 27 341 WSO c s cro Pr RR A E E EA 27 3 4 2 Installation Windows Vista PT 30 35 Installation Ot NArIWEAre acesdn eu ue ta HERO SCHRIEB a EEUU IUS ARER 33 35 1 Attaching the camera ON eciam ad Cu ME MU MM MEME EUM EE MM MM 34 Jor Connecting the camera head to the COMputer Lentemaeatvaba peer tuii eli ER mesh daas
27. Getting Started with ImageQuant LAS 4000 28 9607 59 AB Phenomenon After exposure no image is displayed on the monitor 6 Troubleshooting 6 4 Problems with ImageQuant LAS 4000 Control Software Cause The exposure time is too short Countermeasure Make the exposure time longer The light source is not correct Install the correct light source The filter is not correct Install the correct filter The sample is not in position Check the sample position The focus is not correct Adjust the focus Exposure cannot be performed even if the Start button has been pressed USB cable connection failure Connect the USB cable correctly The LAS High Sens lens is not recog nized Lens cable connection failure Connect the lens cable correctly The light source is not recognized The light source is not con nected correctly Connect the light source correctly The lifting table does not move the tray to the correct position USB cable connection failure Connect the USB cable correctly No button is displayed in Focusing mode Lens cable connection failure Connect the lens cable correctly The wide view lens is installed Adjust the focus manually when using the wide view lens 6 5 Message ImageQuant LAS 4000 hardware error occurred SensKey H SensCode H is displayed If the error persists even after the following countermeasures have been
28. Quant LAS 4000 28 9607 59 AB 6 1 Problems with the exposed image Phenomenon An appropriate image does not appear Cause Image is overexposed 6 Troubleshooting 6 1 Problems with the exposed image Countermeasure Shorten the exposure time or check the iris setting Image is underex posed Make the exposure time longer or check the iris setting The focus is not cor rectly adjusted Adjust the focus correctly The troy lens or filter are dirty Clean the tray lens and filter See Chap ter 5 Maintenance on page 61 The wrong filter is in place Install the correct filter The wrong lens is in stalled Install the correct lens The wrong sample tray is being used Change to the correct sample tray The wrong light source is selected Select the correct light source The Flat Frame file is not exposed correctly Expose the Flat Frame again The size of the object to be exposed does not coincide with the exposed area Place the sample correctly on the sample tray and place the tray at the correct tray position Light leaks on the image The ImageQuant LAS 4000 door is not closed completely Close the ImageQuant LAS 4000 door and expose the image again Exposure to direct sunlight Avoid direct sunlight The image appears misty Dew condensation in the optical system Quit the ImageQuant LAS 4000 Control Software and wait until the o
29. R for the right sides e install the Epi lights in matching pairs only If Epi lights with different wavelengths are used the intended image will not be obtained Getting Started with ImageQuant LAS 4000 28 9607 59 AB 47 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 for exposure Changing or installing a filter Optical filters can be inserted in the filter turrent and registered in the ImageQuant LAS 4000 Control Software as follows 39 ImageQuant LAS 4000 File Edit View Option Help ImageQuant 3 LAS 4000 Change filter and filter position Current Filters Select Filters 1 Through Through 2 Through Through 3 Through Through 4 Through Through 5 Through Through OK Filter down Cancel 1 Ensure that the camera head and PC are connected Turn on the instrument and the PC and start the ImageQuant LAS 4000 Control Software 2 Close the instrument door 3 Select Filter Customization in the Option menu in the ImageQuant LAS 4000 Control Software main window 4 Clickthe Filter down button The filter changer is lowered 5 Open the instrument door 48 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 for exposure 6 Turn the filter changer so the desired position is easily accessible If a filter is already in place at this position unscrew it carefully and put i
30. Read the user documentation before using the system Do not open any N covers or replace parts unless specifically stated in the user documentation The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand C The system complies with applicable European directives Labels concerning use of hazardous substances Label Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment 18 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 2 Safety instructions 2 2 Labels Meaning This symbol indicates that the product contains hazardous materials in excess of the 40s limits established by the Chinese standard SJ T11363 2006 Requirements for Concen NU tration Limits for Certain Hazardous Substances in Electronics Intelligent dark box IDX Camera cable es This device complies with part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation This Class A digital apparatus meets all requiremen
31. Regulatory information 1 2 Regulatory information This section describes the directives and standards that are fulfilled by the ImageQuant LAS 4000 Manufacturing information The Declaration of Conformity includes the following information Requirement Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden May 2009 Identity of person authorized to sign Declaration See EC Declaration of Conformity of Conformity CE Conformity This product complies with the European directives listed in the table by fulfilling the corresponding harmonized standards A copy of the Declaration of Conformity is available on request 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive International standards This product fulfills the requirements of the following standards Standard Description Notes EN 61010 1 IEC 61010 1 UL Safety requirements for electrical equip 61010 1 IEC 61010 2 81 ment for measurement control and lab CAN CSA C22 2 No 61010 1 oratory use EN 61326 1 EMC emissions and immunity require Harmonized with VCCI Class A ments for electrical equipment for mea 2004 108 EC FCC Part 15 B Class A surement control and laboratory use ICES 0
32. are installations you may be asked to confirm your actions in a dialog with thetext Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Openthe control panel and click Classic View in the upper left corner 2 Open the Add Hardware tool 3 Inthe Add Hardware dialog click the Next button 4 Select Install the hardware that manually select from a list Advanced and click the Next button 30 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Installation 3 4 Installation of the software 3 4 2 Installation Windows Vista 5 Select Show All Devices and click the Next button From the list below select the type of hardware you are installing If you do not see the hardware category you want click Show All Devices Common hardware types yg Display adapters Ci IDE ATA ATAPI controllers A IEEE 1284 4 compatible printer GER IEEE 1284 4 devices IEEE 1394 Bus host controllers E Imaging devices IF Infrared devices Wl adis Contar Futander 6 Click the Have Disk button 7 Insert the ImageQuant LAS 4000 Control Software CD and click the Browse button 8 Select to install the driver from the ImageQuant LAS 4000 Control Software CD Lookin 9 DVD CD RW Drive D ImageQuant LAS mg 6f em S Recent Items Deskt ANO ur EY Network Recent Places admin bs Public mE j Computer Floppy D
33. e ImageQuant LAS 4000 msi 3 Inthe ImageQuant LAS 4000 InstallShield Wizard click the Next button 4 Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see the back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button Getting Started with ImageQuant LAS 4000 28 9607 59 AB 29 3 Installation 3 4 Installation of the software 3 4 1 Installation Windows XP 5 Select destination folder in the dialog ie ImageQuant LAS 4000 InstallShield Wizard Destination Folder Click Next to install to this Folder or click Change to install to a different Folder gt Install ImageQuant LAS 4000 to C Program Files Click the Next button to install the software at the default folder C Program Files Click the Change button to install to a different folder 6 Click the Install button 7 Clickthe Finish button The installation of ImageQuant LAS 4000 Control Software is now completed 3 4 2 Installation Windows Vista Installation is performed in the following sequence 1 Installation of FUJI USB Control driver 2 Installation of FUJI USB Function driver 3 Installation of ImageQuant LAS 4000 Control Software 3 4 2 1 Installation of the USB Control Driver Windows VISTA Note The computer and ImageQuant LAS 4000 must not be connected with a USB cable during the operation Note During softw
34. e from the wall socket WARNING Access to power switch and power cord Do not block the rear and side panel of the instrument The Power switch must always be easy to access The power cord must always be easy to disconnect 2 4 Recycling information The equipment must be clean from contaminants before decommissioning and all local regulations must be observed with regard to waste disposal Disposal general instructions When taking the ImageQuant LAS 4000 out of service the different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances The lamps in the ImageQuant LAS 4000 may contain mercury vapour These must be disposed of in manner compliant with local regulations 24 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 2 Safety instructions 2 4 Recycling information Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of the equipment Getting Started with ImageQuant LAS 4000 28 9607 59 AB 25 3 Installation 5 Installation This chapter provides information regarding installation of the ImageQuant LAS 4000 5 Site requirements The following table gives a summary of power supply and environ
35. eader software is in Adobe Reader is needed stalled Failed to open End User Licence Agreement Please note that a PDF reader e g Adobe Reader is needed Failed to open the Getting Started guide Please note that a PDF reader e g Adobe reader is needed Getting Started with ImageQuant LAS 4000 28 9607 59 AB 77 6 Troubleshooting 6 6 Error messages and countermeasures Edit menu preference Print Adjust Error message Meaning Countermeasure The range of value is 90 110 The supplied value is not Enter a suitable value Please set a suitable value within the appropriate interval 78 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 7 Reference information Reference information 7 1 Main specifications CCD 3 200 000 pixels Fujifilm super CCD Cooling temperature 30 C Number of gradations Recorded image 16 bit Focusing 8 bit Exposure time 1 100 seconds to 2 hours Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000 Control Software Lens High sensitivity lens F0 85 43 mm Wide view lens F1 8 24 mm Shading correction Software system Maximum sample size 250 x 250 mm with F1 8 24 mm lens 210 x 140 mm with F0 85 43 mm lens Dynamic range 4 orders of magnitude Maximum image size 12 6 MB Getting Started with ImageQuant LAS 4000 28 9607 59 AB 79 7 Reference information 7 1 Main specifica
36. equires CCD calibration Please run CCD calibration for the exposure time range from 1 100 sec to 2 hours No CCD calibration file ex ists Create a correction file using the CCD Calibration function in the Option menu Perform calibration for both over 2 hours and from 1 100 sec to 2 hours Getting S arted with ImageQuant LAS 4000 28 9607 59 AB 6 Troubleshooting 6 6 Error messages and countermeasures Error message Meaning Countermeasure NP Tray can only be used in tray po The NP tray can be used Place the NP tray at tray posi sition 3 with the FUJINON VRFA3LMD only with the LAS High tion 3 Use the LAS High Sens lens and the chemiluminescence Sens lens and attray posi lens method tion 3 NP tray is not in the proper position Please set the tray so that contactis The tray is not detected Insert the tray and ensure it is made with the sensor at the rear of pushed fully in the cabinet Start button during exposure Error message Meaning Countermeasure Cannot continue with the automatic The automatic exposure Change the settings appropri exposure function The sample may functionisunabletocalcu ately or perform an exposure be out of the photometric range or late a correct exposure in Manual mode a setting may need to be adjusted time Exposure has been stopped The UV transilluminator Reduce the total exposure time The UV illuminator was turned of
37. f Was turned off so that the UV transilluminator because the 10 minute limit was is lit for less than 10 minutes reached Attention The door was opened The door was opened dur The image may be incorrectly during exposure ing exposure exposed Perform the exposure again Start button after exposure Error message Meaning Countermeasure No image file was saved Do you No images were saved Press OK to discard the want to continue recorded imagels or Cancel to go back and save the images The save and launch function failed Failed to launch the exter Choose an application to nal application The desig launch in Launch Application nated file cannot be found in Preference in the Edit menu Getting Started with ImageQuant LAS 4000 28 9607 59 AB 75 6 Troubleshooting 6 6 Error messages and countermeasures Program settings button Error message Cannot save The maximum number of registered program templates has been reached Meaning There are too many exist ing program template files Countermeasure Remove some program tem plate files that are no longer required A program template with the same name already exists Do you want to overwrite it A file with the same name already exists Change the filename or choose to overwrite the older file You cannot specify the first character of template name as or Please start with another character
38. g Epi lights If the tray is higher then it should be lowered to tray position 4 using the ImageQuant LAS 4000 Control Software before attaching the lights as described below Latch Epi light base If the tray is at Tray position 4 proceed to step 3 Otherwise close the IDX door Click on Method Tray position set Tray position to 4 and click the OK button Open the IDX door If no Epi light is currently in place inside the instrument proceed to step 7 FW N e Spring up the latch to unlock the Epi light Getting Started with ImageQuant LAS 4000 28 9607 59 AB 45 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 for exposure 5 Holding the latch with one hand remove the Epi light by pulling it gently outwards 7 Beforeinstalling the new Epi light ensure that the latch is sprung open upwards 8 Hook the hanger located on top of the Epi light to the top edge of the Epi light base 1 Two guide holes are located on the back of the Epi light base 2 Slide the hook after attaching the guide pins to the guide holes 46 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 2 Preparation 4 2 2 Preparing the ImageQuant LAS 4000 for exposure 9 Push the Epi light while holding the latch open and slide it inwards until a click sound is heard 11 Repeat this process for the opposite side Note TheEpilights are clearly labelled L for the left and
39. hermal noise level resulting in greater sensitivity The F0 85 LAS High Sens lens has remote focus and iris Chemiluminescence is detected at high sensitivity using a four step binning algorithm Fluorescence is detected at high sensitivity using either a UV transilluminator or one of several Epi light sources Getting Started with ImageQuant LAS 4000 28 9607 59 AB 1 Introduction 1 3 The ImageQuant LAS 4000 mage capture and analysis is simplified using the ImageQuant LAS 4000 Control Software in combination with GE Healthcare s analysis software ImageQuant TL ImageQuant LAS 4000 hardware components The following illustrations and table show the main hardware components of the ImageQuant LAS 4000 5 Intelligent dark box IDX IDX interior Part Name Description 1 Camera head CCD cooling and image data output 2 Intelligent dark box IDX Dark box 3 Lens F0 85 43 mm LAS High Sens lens or F1 8 24 mm wide view lens 4 Epi light source NIR Epi light 710 nm Red Epi light 630 nm Green Epi light 520 nm Blue Epi light 460 nm UV Epi light 365 nm White Epi light Getting Started with ImageQuant LAS 4000 28 9607 59 AB 9 1 Introduction 1 3 The ImageQuant LAS 4000 Part Name Description 5 Trans light source 312 nm UV transilluminator White light table 6 Filter IR785 Alexa filter for NIR LED R670 Cy5 filter for red LED
40. ime when using the UV light is 60 seconds Please input the file name using the No file name is given in Input a valid filename in the program settings window Program Settings Program Settings dialog Please input a valid exposure time The exposure time isnot Choose a suitable exposure appropriate time Cannot start the exposure In the Digitize Method the Change the sensitivity setting Automatic exposure is only available Auto function cannot be or expose an image in Manual when using standard sensivity in the used when the sensitivity mode digitization method is High Super or Ultra Automatic exposure cannot be used at the sensitivity or resolution Getting Started with ImageQuant LAS 4000 28 9607 59 AB 73 6 Troubleshooting 6 6 Error messages and countermeasures 74 Error message Please input a valid repeat limit count Meaning The number of exposures is not approriate in Incre ment or Repetition mode Countermeasure Change the number of expo sures or repeat limits to a suit able quantity CCD Cooling is not stable Dark frame correction will not be ac curate Do you want to continue CCD calibration will not be accurate since the temper ature is not stable when exposing an EtBr sample before the CCD has reached its target cooling temperature Click the OK button if you wish to take an exposure without correction Can not start the exposure beca
41. inish button The installation of ImageQuant LAS 4000 Control Software is now completed 5 5 Installation of hardware This section details the assembly that is required in order to use the ImageQuant LAS 4000 33 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 34 3 Installation 3 5 Installation of hardware 3 5 1 Attaching the camera head 3 5 1 Attaching the camera head The camera head is attached to the IDX as follows 1 Install the camera head while pushing the lens release button against this side s wall surface of the camera hole Two screws 3 Connectthe camera cable and USB cable Camera cable 4 Proceed to install the lens See Section 3 5 3 Installing the lens on page 35 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Installation 3 5 Installation of hardware 3 5 2 Connecting the camera head to the computer 3 5 2 Connecting the camera head to the computer Connect the USB cable from the USB connector of the ImageQuant LAS 4000 camera head to one of the USB ports of the computer Analysis PC Optional Instrument Note Usethe USB cable supplied with the equipment Do not connect via a USB hub Do not use other USB equipment on the PC while ImageQuant LAS 4000 is in use This may result in the loss of image data 3 5 3 Installing the lens This section describes how to install the L
42. ion to 4 and click the OK button 3 Open the IDX door 4 Ensure that the latch is sprung open upwards 40 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Installation 3 5 Installation of hardware 3 5 6 Installing Epi light sources 5 Hook the hanger located on top of the Epi light to the top edge of the Epi light base 1 2 No Action 1 Two guide holes are located on the back of the Epi light base 2 Slide the hook after attaching the guide pins to the guide holes 6 Push the Epi light while holding the latch open and slide it inwards until a click sound is heard 8 Repeat steps 4 7 for the opposite side Note e The Epi lights are clearly labelled L for the left and R for the right sides e install the Epi lights in matching pairs only If Epi lights with different wavelengths are used the intended image will not be obtained Getting Started with ImageQuant LAS 4000 28 9607 59 AB 41 4 Operation 4 Operation This chapter describes how to operate the ImageQuant LAS 4000 and how samples are to be placed in the instrument The steps required to record an image are also described 4 1 Operation overview The general flow of operation is as follows Turn on the instrument and computer Preparation Start the Control Software Place the sample Exposure Expose the sample Analyze the image 4 2 Preparation 4 2 1 Starting the ImageQuant LAS 4000 and Control Sof
43. is open Close the door The subsequent processing should start 66 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 6 Troubleshooting 6 2 Strange sounds or odors are perceived Phenomenon Cause Countermeasure ImageQuant LAS 4000 beeps on and A hardware error oc See the countermeasures for the SensKey and off three times and the ERROR LED curred SensCode numbers displayed on screen of the lights analysis PC in Section 6 5 Message ImageQuant LAS 4000 hardware error occurred on page 69 ImageQuant LAS 4000 generates a ImageQuant LAS 4000 Turn off the ImageQuant LAS 4000 power strange odor failure switch immediately and contact your GE Healthcare representative ImageQuant LAS 4000 emits smoke ImageQuant LAS 4000 Turn off the ImageQuant LAS 4000 power failure switch immediately and contact your GE Healthcare representative 6 3 Problems with ImageQuant LAS 4000 If the error persists even after the following countermeasures have been taken fill in the necessary items and the error code in the Service report fax sheet at the end of the User Manual Phenomenon LED lights on ImageQuant LAS 4000 do not light during startup diagnosis Cause Power cable installation failure Countermeasure Connect the power cable correctly The ImageQuant LAS 4000 door cannot be opened and closed The door cannot be locked Foreign objectis present in the locking section or door sensor
44. isk Drive 4 PEEP Local Disk C di Windows d System32 admin e Tru Computer Network te modif Location e STORE N GO E File name inf Files of type Setup Information inf 9 Open the USB Control folder Lookin DVD CD RW Drive D ImageQuant LAS 401 v o I md a Q Name Size Type Date modif Location ay Files Currently on the Disc 2 Recent Places USB Control File Folder USB Function File Folder File name int Files of type Setup Information inf Getting Started with ImageQuant LAS 4000 28 9607 59 AB 31 3 Installation 3 4 Installation of the software 3 4 2 Installation Windows Vista 10 Select the file DevMng and click the Open button S Locate File x Look in J USB Control OP By T Name Date modif Type Size ap 5m RecentPlaces 77 Desktop test Computer A gt Network File name DevMng Files of type Setup Information i Cance 11 Click the OK button in the Install From Disk dialog 12 Click the Next button in the Add Hardware dialog 13 Click the Next button once more in the Add Hardware dialog 14 Click Install this driver software anyway i Windows Security amp Windows can t verify the publisher of this driver software gt Don t install this driver software You should check your manufacturer s website for updated driver software for your device 1 gt Install this driver
45. lace the instrument at least 20 cm away from walls or other equipment to ensure adequate cooling gt l P gt i P gt CAUTION Do not open the door of the instrument too wide and do not apply excessive force to it Getting Started with ImageQuant LAS 4000 28 9607 59 AB 2 Safety instructions 2 1 Safety precautions CAUTION When opening or closing the door use the opening lever Take care not to catch objects or fingers in the door when closing CAUTION Do not place heavy objects on the equipment These may fall and cause injury CAUTION Only one ImageQuant LAS 4000 should be connected to a particular PC Connecting more than one may result in malfunction CAUTION Turn the power switch off before connecting or disconnecting cables otherwise the equipment may malfunction CAUTION Do not connect any lens or light source other than those recommended by GE Healthcare CAUTION Turn the power switch off before attaching or detaching a lens otherwise the equipment may malfunction CAUTION Do not leave the lens hanging from the lens guide It may fall and damage the equipment CAUTION Do not touch the shutter on the camera head Damage to the shutter will impede correct operation gt gt E gt B gt gt Be D Getting Started with ImageQuant LAS 4000 28 9607 59 AB 15 2 Safety instructions 2 1 Safety precautions C
46. loth dampened with mild detergent then wipe with a soft dry cloth WARNING Wear gloves while cleaning There may be residues of hazardous chemicals 5 5 Regular inspections Regularly inspect the ImageQuant LAS 4000 to ensure the following 62 The power supply plug is firmly secured in the power outlet The power cable and supply plug do not become overheated The power cable is not damaged in any way The ventilation holes in the equipment are free from dust and dirt Getting Started with ImageQuant LAS 4000 28 9607 59 AB 5 Maintenance 5 5 Regular inspections Thelights do not remain lit and the tray rail or filter changer do not move when the IDX door is opened Getting Started with ImageQuant LAS 4000 28 9607 59 AB 63 6 Troubleshooting 6 Troubleshooting 64 This chapter describes various problems that can foreseeably occur with the ImageQuant LAS 4000 and ImageQuant LAS 4000 Control Software Suggestions of possible countermeasures are given Take the following procedures if an error occurs 1 Take note of the error code and error message on the monitor 2 Turn off the power to ImageQuant LAS 4000 and the analysis PC then turn them on again after about ten seconds 3 If the error persists fill in the necessary items and the error code in the Service report fax sheet at the end of the ImageQuant LAS 4000 User Manual and then contact your GE Healthcare representative Getting Started with Image
47. lug the equipment from the power outlet then contact you local GE Healthcare representative WARNING Do not drop or otherwise damage the camera head or ImageQuant LAS 4000 This may result in fire or electric shock Turn off the power switch unplug the equipment from the power outlet then contact you local GE Healthcare representative Getting Started with ImageQuant LAS 4000 28 9607 59 AB 2 Safety instructions 2 1 Safety precautions WARNING Do not damage the power supply cord by bending twisting heating or allowing them to become pinned under the equipment Using damaged power cords could result in fire or electric shock gt If the power supply cords are damaged contact your local GE Healthcare representative for replacements WARNING Do not place the equipment on unstable tables or on inclined surfaces as the equipment could be dropped or fall resulting in injury CAUTION Do not place the equipment or parts in direct sunlight This may degrade performance or result in overheating and fire CAUTION Do not scratch or drop parts containing glass such as lenses filters or lights gt bP gt Personal protection WARNING Parts of this equipment emit UV radiation Avoid exposure Wear protective clothing and eyewear UV radiation can cause severe burns and long term injury to the skin and eyes gt Excessive exposure of the skin can cause premature
48. mental requirements For detailed information refer to Section 7 1 Main specifications on page 79 Parameter Requirement Power supply 13 A 100 120 V or 10 A 200 240 V Line frequency 50 to 60 Hz Ambient temperature 15 C to 28 C fluctuation lt 10 C h Storage 25 C to 70 C Placement Stable laboratory bench load capacity gt 100kg m At least 20 cm free on all sides Humidity 3096 to 7096 non condensing Storage 596 to 100 non condensing 3 2 Transport The ImageQuant LAS 4000 weighs approximately 57 kg and requires at least two people to lift and move it Before moving the instrument 1 Turn off the instrument 2 Disconnect the power cord 3 Remove the lens transmitted light source and any loose parts from inside of the IDX Refer to the ImageQuant LAS 4000 User Manudl 5 5 Unpacking When unpacking e Check the equipment for any apparent damage before installing 26 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Installation 3 3 Unpacking Document any damage carefully and contact your GE Healthcare representative 5 4 Installation of the software 3 4 1 Installation Windows XP Installation is performed in the following sequence 1 Installation of USB Control driver 2 Installation of USB Function driver 3 Installation of ImageQuant LAS 4000 3 4 1 1 Installation of the USB Control Driver Windows XP Note The computer and ImageQuant LAS 4000 must not
49. od E 3 5 4 Attaching a filter to the filter changer This section describes how to attach a filter to the filter changer and how to register the filter at the chosen filter changer position in the ImageQuant LAS 4000 Control Software Note Before installing or changing filters in the filter changer the ImageQuant LAS 4000 Control Software should be installed and running For instructions on installing the software refer to Section 3 4 Installation of the software on page 27 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 37 3 Installation 3 5 Installation of hardware 3 5 4 Attaching a filter to the filter changer 32 ImageQuant LAS 4000 File Edit View Option Help ImageQuant 3 LAS 4000 Change filter and filter position Current Filters Select Filters 1 Through Through 2 Through Through 3 Through Through 4 Through Through 5 Through Through OK Filter down Cancel 1 Ensure that the camera head and PC are connected Turn on the instrument and the PC and start the ImageQuant LAS 4000 Control Software 2 Close the instrument door 3 Select Filter Customization in the Option menu in the ImageQuant LAS 4000 Control Software main window 4 Click the Filter down button The filter changer is lowered 5 Open the instrument door 38 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Installation 3 5 Installation of hardware 3 5 4
50. or resolution More than 1280 x 1024 pixels 7 5 Literature For further information on the ImageQuant LAS 4000 and the ImageQuant LAS 4000 Control Software refer to the ImageQuant LAS 4000 User Manual 7 4 Ordering information For ordering information visit www gelifesciences com quantitative_imaging 84 Getting Started with ImageQuant LAS 4000 28 9607 59 AB For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com quantitative imaging imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company ImageQuant is a trademark of GE Healthcare companies All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Oct 2009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan C
51. orporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9607 59 AB 11 2009
52. ower supply connector Diffusion board White light table e SN FUJIFILM UV transilluminator UV transilluminator n FUJIFILM Power supply connector Mercury containing Products Label contain mercury wmm Dispose of properly Ultraviolet rays lamps gt UV glass filter S e 22 Getting Started with ImageQuant LAS 4000 28 9607 59 AB Cal plates 2 Safety instructions 2 2 Labels ES CAUTION VSOMETICUTH EF Et Y LTG 2 MED IGN RTT NI TOROS amp HBL TEAL T EW A 3 NEIL PETAL EO obe LF HEL T Raw 1 Set this place on the tray with this surface facing down 2 Make sure to confirm that there is no dust or dirt on the top surface opposite of this surface prior to use 3 When this plate becomes durty wash it with neutral detergent take care not to scratch it Sample trays The Epi tray is shown here The White trans tray and UV trans tray are labelled correspondingly Epi tray FUJIFILM Getting Started with ImageQuant LAS 4000 28 9607 59 AB 23 2 Safety instructions 2 2 Labels Filters The 605DF40 filter is shown here Other filters are labelled according to their type CS 605DF40 605 EtBr filter SN FUJIFILM Filter cover 2 5 Emergency procedure In an emergency situation Turn off the ImageQuant LAS 4000 power switch e Disconnect the power cabl
53. peration en vironment meets the required specifica tions Getting Started with ImageQuant LAS 4000 28 9607 59 AB 65 6 Troubleshooting 6 1 Problems with the exposed image Phenomenon age Unevenness is seen on the im Cause ness Light source uneven Countermeasure Check that both left and right Epi light sources are correctly in place and that they are of the same type A lamp in the UV tran silluminator is broken Turn the power off and contact your GE Healthcare representative The Flat Frame is not Expose the Flat Frame again correctly exposed There is noise on an image ex Degradation of UV Purchase a new UV trans tray posed using UV trans tray trans tray 6 2 Strange sounds or odors are perceived Phenomenon The camera head generates a strange noise Cause Camera head failure Countermeasure Turn off the ImageQuant LAS 4000 power switch immediately and contact your GE Healthcare representative The camera head generates a strange odor Camera head failure Turn off the ImageQuant LAS 4000 power switch immediately and contact your GE Healthcare representative The camera head emits smoke Camera head failure Turn off the ImageQuant LAS 4000 power switch immediately and contact your GE Healthcare representative ImageQuant LAS 4000 beeps on and off four times at intervals of three minutes and the Busy LED blinks The door
54. portation storage conditions Temperature 25 C to 70 C Humidity 5 96 to 100 96 RH no dew condensation Installation location conditions 1 Donotinstall the equipment in an area where the temperature varies widely Do not install the equipment near a source of heat Do not install the equipment in an area where it may get wet or flooded Do not install the equipment in an area where it may be exposed to corrosive gas Do not install the equipment in a dusty area Dn Ui f t N Do not install the equipment in an area constantly or excessively exposed to os cillations or impacts 7 Donotinstall the equipment in an area exposed to direct sunlight Operation site Indoors Maximum operating altitude 2000 m or lower Overvoltage category category l Transient overvoltage Rated pollution applied Pollution Degree 2 Analysis unit interface USB 2 0 Do not connect the ImageQuant LAS 4000 USB connector to a computer not certified with UL60950 1 UL listed and IEC60950 1 Others Noise 70 dB A or lower Getting Started with ImageQuant LAS 4000 28 9607 59 AB 83 7 Reference information 7 2 Minimum computer requirements 7 2 Minimum computer requirements Operating system Windows XP SP3 Or Windows Vista Business SP1 32 bit Memory More than 1 GB Processor Intel Core 2 Duo processor HD More than 80 GB USB port version USB 2 0 Optical drives DVD ROM Monit
55. rect operation of your system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Continue Anyway STOP Installation 17 Click the Finish button to complete the driver installation 3 4 1 2 Installation of the USB Function driver Windows XP 1 Connect the computer and the ImageQuant LAS 4000 with a USB cable and turn ON the power switch of ImageQuant LAS 4000 Result The ImageQuant LAS 4000 will automatically be detected by the computer 2 Inthe Found New Hardware Wizard dialog choose No not this time and click the Next button Found New Hardware Wizard Welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the Windows Update Web site with your permission Read our privacy policy Can Windows connect to Windows Update to search for software O Yes this time only O Yes now and every time connect a device Click Next to continue 3 Insert the ImageQuant LAS 4000 CD then select Install the software automatically Recommended and click the Next button 4 Click the Finish button to complete the installation 3 4 1 3 Installation of ImageQuant LAS 4000 Control Software Windows xP 1 Insert the ImageQuant LAS 4000 Control Software CD 2 Locate and double click the fil
56. s Intel AIM Codec B Modem Back Nest Cancel 8 Click the Have Disk button 9 Insert the ImageQuant LAS 4000 CD and click the Browse button 10 Select to install the driver from the ImageQuant LAS 4000 CD Lock rc CO ImageQuant LAS 4000 1D oS 3 Ms Rlecert Documents per My Documents My Computer Fle pme i MyNemok Flee ol ew Cored Look rc 2 ImepeQuset LAS 8000 D 1 od QUSE Fenton My Recent Documents Desktop Mp Document My Computer Fle pme a Qe MyNemok Fes of yoe Corel 12 Select the DevMng inf file and click the Open button Look rc E USB Commot 02cm BEIM My Fiecerk Documents j Desktop My Documents My Computer Fle pane DEVMNG C MyNeleok Flee of bree Cancel 28 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 3 Installation 3 4 Installation of the software 3 4 1 Installation Windows XP 13 Click the OK button in the Install From Disk dialog 14 Click the Next button in the Add Hardware Wizard 15 Click the Next button once again in the Add Hardware Wizard 16 Click the Continue Anyway button Hardware Installation The software you are installing for this hardware Fujifilm USB Device Managment Driver has not passed Windows Logo testing to verify its compatibility with Windows XP Tell me why this testing is important Continuing your installation of this software may impair or destabilize the cor
57. software anyway i Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information i v See details 15 Click the Finish button 3 4 2 2 Installation of the USB Function driver Windows VISTA Note During software installations you may be asked to confirm your actions in a dialog with thetext Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation 1 Connectthe computer and the ImageQuant LAS 4000 with a USB cable and turn ON the power switch of ImageQuant LAS 4000 Result The ImageQuant LAS 4000 will automatically be detected by the computer and the Plug and Play function in Windows Vista starts 2 Inthe Found New Hardware dialog select Locate and install driver software recommended 3 Insert the ImageQuant LAS 4000 Control Software CD and click the Next button in the Found New Hardware dialog 32 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 5 3 Installation 3 4 Installation of the software 3 4 2 Installation Windows Vista Select Install this driver software anyway n K suce amp Windows can t verify the publisher of this driver software Don t install this driver software You should check your manufacturer s website for updated driver software for your device Install this driver software any
58. t back in its cover 7 Screw the new filter into place 8 Select the appropriate filter from the drop down list and click the OK button Change filter and Filter position Current Filters Select Filters 1 Through Through v 2 Through Thi w Through 605DF40 Y515 Di 4 Through Thi 510DF10 3 Through Thi 5 Through Thi TRIES R670 575DF20 Filter down l L Note The filter changer returns to the original position when you click the Start Focusing or Method Tray position buttons 4 2 3 Placing the sample This section describes how to choose an appropriate sample tray and how to place the sample for exposure 1 Select a sample tray suitable for the type of exposure to be performed Detection Sample type Tray Chemiluminescence Membrane Epi tray Bioluminescence Titer plate NP tray Fluorescence Gel UV Trans illumination UV trans tray Gel Epi illumination Epi tray Membrane Epi tray Getting Started with ImageQuant LAS 4000 28 9607 59 AB 49 4 Operation 4 2 Preparation 4 2 5 Placing the sample Detection Sample type Digitization Membrane Epi tray Gel Coomassie silver stain White trans tray 2 Place a sample on the sample tray For Epi tray a Place the sample directly on the tray For UV or White Trans trays a Cut out a gel sheet slightly larger than the sample size b Place the gel sheet on the Trans tray AE Gel sheet
59. t conditions Free space required around ImageQuant LAS 4000 Secure space for maintenance work as follows Front 600mm Right 600mm Left 600 mm Rear 200mm Top 1000 mm Table strength The allowable load must be 981 N m 100kg m or greater Other conditions 1 Decide on an installation location taking into consideration the work flow and ancillary facilities to be used 2 Required construction work and electricity air conditioning work must be complet ed in advance 3 It is not desirable to have a heat source on the IDX right side face where there is an air intake fan even if the environmental requirements have been met 4 Donotinstallthe equipment near a window to avoid direct sunlight Attach a blind to nearby windows 5 Donot place objects near the power outlet so that you can disconnect the power cable from the outlet in case of emergency Floor oscillation conditions 1 Operating time Oscillation 0 03G 5 to 60 Hz Impact 1G 2 Non operating time Oscillation 0 4G 5 to 60 Hz Impact 2G Getting Started with ImageQuant LAS 4000 28 9607 59 AB 7 Reference information 7 1 Main specifications Environmental conditions Operating temperature humidity conditions Temperature 15 C to 28 C with temperature fluctuation below 10 C per hour or lower Humidity 3096 to 70 RH_ no dew condensation When the above conditions cannot be satisfied modify the facilities accordingly Trans
60. tained sample The general procedure is however similar for all types of sample For detailed instructions refer to the ImageQuant LAS 4000 User Manual Images may also be obtained by repeated incremental or programmed exposure of a sample For detailed instructions on these types of exposure refer to the ImageQuant LAS 4000 User Manudl 4 3 1 Workflow The workflow for exposing a sample and recording an image is as follows Getting Started with ImageQuant LAS 4000 28 9607 59 AB Choose method and tray position Adjust the focus Set exposure sensitivity Save the image 4 3 2 Choose method and tray position Step Action 4 Operation 4 3 Performing an exposure 4 3 1 Workflow 1 Click the Method Tray position button Result The Method Tray position dialog opens Getting Started with ImageQuant LAS 4000 28 9607 59 AB 53 4 Operation 4 5 Performing an exposure 4 3 2 Choose method and tray position Step Action 2 Select a Method for exposure Method Tray position Method Light Filter Iris Chemiluminescence None Through F0 85 Fluorescence 605DF40 F2 8 O Digitization Epi illumination White Epi illumination Through F2 8 Trans illumination White Trans illumination Through F2 8 Detection Method Chemiluminescence Chemiluminescence Bioluminescence Fluorescence using UV transilluminator 312 nm Fluorescence EtBr Fluorescence using UV Epi light
61. taken fill in the necessary items and the error code in the Service report fax sheet at the end of the User Manual and then contact your GE Healthcare representative SensKey SensCode Content Countermeasure 8000 UV exposure error If the problem remains restart the ImageQuant LAS 4000 and ImageQuant LAS 4000 Control Software 2 9100 Temperature adjustment er Turn off the ImageQuant LAS 4000 power for ror awhile After that restart the ImageQuant LAS 4000 and ImageQuant LAS 4000 Control Soft ware Getting Started with ImageQuant LAS 4000 28 9607 59 AB 69 6 Troubleshooting 6 5 Message ImageQuant LAS 4000 hardware error occurred SensKey SensCode Content Countermeasure B001 Lifting table error during Restart the ImageQuant LAS 4000 and Image startup diagnosis Quant LAS 4000 Control Software B002 Filter changer error during startup diagnosis BOO4 Focus error during startup diagnosis B008 Iris error during startup diag nosis B010 Epi light source error during startup diagnosis B020 UV transluminator error dur ing startup diagnosis B040 White light table error during startup diagnosis 9 BO A combination of errors B004 except described to B020 above are generated above during startup diagnosis B101 Lifting table error B102 Filter changer error B104 Focus error B108 Iris error B110 Transmitted light source error B120 UV transilluminator error B
62. ting Started with ImageQuant LAS 4000 28 9607 59 AB 6 Troubleshooting 6 6 Error messages and countermeasures Error message Meaning Countermeasure Cannot start flat frame calibration The CCD has not been cali Performa flat frame calibration because the CCD calibration for High brated Resolution mode does not exist Please run CCD calibration first Option menu CCD Calibration Error message Meaning Countermeasure The door was opened during expo The door was opened dur Perform the CCD calibration sure This image may be inaccurate ing exposure sotheimage again may not have been ex posed correctly Option menu CCD Calibration Pro Error message Meaning Countermeasure Cannot use the flat frame calibration The FlatFrame Calibration Use the CCD Calibration option for the following settings Light none Pro function cannot be Filter No1 FUJINON VRF43LMD lens used in chemilumescent mode Cannot continue with the automatic The automatic exposure Change the settings appropri exposure function The sample may functionisunabletocalcu ately or perform an exposure be out of the photometric range or late a correct exposure in Manual mode a setting may need to be adjusted time Help menu Error message Meaning Countermeasure Failed to open the User Manual The PDF documents can Ensure that the documentation Please note that a PDF reader e g not be opened and PDF r
63. tions Light source Epi class 1 light source Blue Epi light 460 nm Green Epi light 520 nm Red Epi light 630 nm NIR Epi light 710nm Epi and Trans class 1M light source UV Epi light 365 nm UV transilluminator 312nm Size 322x 313 x 87 mm UV transmitted filter size 200 x 200 mm White transmitted light table White LED Size 322x 313 x 87 mm External dimensions Camera head 224x161x252 mm W H D IDX 510 x 730 x 480 mm W H D Weight Camera head 3 4 kg IDX 49 kg not including the light source lens and tray High sensitivity lens 4 5 kg 80 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 7 Reference information 7 1 Main specifications Power requirements Input voltage 100 240 V Voltage variation 10 Phase Single phase Power frequency 50 60 Hz Rated input current 3 0 1 5 A AC power cable Use the cable supplied with the instrument Specifications of cables required for the use of ImageQuant LAS 4000 Voltage Plug connector Cable Power supply cord length Voltage Plug connector Cable Power supply cord length 100 120 V 125V AC 13A SJT3 x 16AWG 60 C Maximum 3 m 200 240 V 250V AC 10A CENELEC OC 3 x 1 0 mm 70 C Maximum 3 m Getting Started with ImageQuant LAS 4000 28 9607 59 AB 81 7 Reference information 7 1 Main specifications Installation conditions 82 Placemen
64. ts of the Canadian Interference Causing Equipment Regulations Cet appareil numerique de la classe A respecte toutes les exigences du Reglement sur le materiel bouilleur du Canada re Bio Sciences AB LUMINESCENT IMAGE ANALYZER Model ImageQuant LAS 4000 Serial No Frequency 50 60 Hz Supply Voltage 100 240 V p us Max Current 30A LSA USTED Protection Closs 1P20 S S CLEMENT Manufacturing Year ACEZO weucver FUJIFILM Corporation TOKYO 106 8620 JAPAN MADE IN JAPAN 405N101506 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 19 2 Safety instructions 2 2 Labels Camera head Camera Head Mechanical shutter Bottom Connector for IDX LAS High Sens lens P r0 85 43mm LAS High Sens Lens 6 SN ME ruri o Label Back side End cap Vm cw ws Lens control connector Lens cap 20 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 2 Safety instructions 2 2 Labels Epi lights The following diagram shows the UV Epi lights Other Epi light pairs are labelled corresponding to their type Guide pin Optical filter and LED Retaining screw hole 1 position Connector i UV Epi light FUJIFILM Getting Started with ImageQuant LAS 4000 28 9607 59 AB 21 2 Safety instructions 2 2 Labels White light table 0 P
65. tware The ImageQuant LAS 4000 and Control Software are started as follows 42 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 4 Operation 4 2 Preparation 4 2 1 Starting the ImageQuant LAS 4000 and Control Software 1 Turn on the ImageQuant LAS 4000 and computer and any auxiliary equipment Note The ImageQuant LAS 4000 and computer may be switched on in any order 2 Start the ImageQuant LAS 4000 Control Software Result A status message is displayed while the ImageQuant LAS 4000 Control Software is starting 3 Once the software has started wait for the CCD camera to cool The status of the CCD is shown in the main ImageQuant LAS 4000 Control Software window When ready the status will change from NOT READY to READY NOT READY F 200 C Note When the CCD camera is ready for use the Power LED lamp on the IDX lights blue Note Under normal operation conditions the cooling temperature of the CCD camera is set to 25 C Note The Method Tray position and Focusing may be set even if the CCD is not ready A sample can be placed during the time taken for the CCD to cool 4 2 2 Preparing the ImageQuant LAS 4000 for exposure Changing the lens Two lenses can be used with the ImageQuant LAS 4000 an F0 85 43mm LAS High Sens lens and an optional F1 8 24mm wide view lens The wide view lens can image samples up to 250 x 250 mm in size For all other applications the LAS High Sens lens is recommended F0 85
66. ual Getting Started with ImageQuant LAS 4000 provides instructions needed to handle the ImageQuant LAS 4000 in a safe way Basic operating instructions are also provided For details refer to the ImageQuant LAS 4000 User Manual Prerequisites In order to operate the ImageQuant LAS 4000 in the way it is intended the following prerequisites must be fulfilled e You should have a general understanding of how a PC and Windows work You must read the safety instructions outlined in the user documentation You should be acquainted with the use of general laboratory equipment and with handling of biological materials In this chapter This chapter contains important user information a general description of the ImageQuant LAS 4000 and its intended use 1 1 Important user information Read this before using the ImageQuant LAS 4000 l All users must read the safety instructions in the ImageQuant LAS 4000 user documentation before installing using or maintaining the equipment Do not operate the ImageQuant LAS 4000 in any other way than described in the user documentation Otherwise you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Getting Started with ImageQuant LAS 4000 28 9607 59 AB 5 1 Introduction 1 1 Important user information Intended use The ImageQuant LAS 4000 is a camera system that produces digital images of chemiluminescent dyed or fl
67. uorescent gels and membranes The ImageQuant LAS 4000 is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices This user documentation contains WARNINGS CAUTIONS and NOTICES concerning the safe use of the product See definitions below Warnings WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE o NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product TIP A tip contains useful information that can improve or optimize your procedures Typographical conventions Software items are identified in the text by bold italic text colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch 6 Getting Started with ImageQuant LAS 4000 28 9607 59 AB 1 Introduction 1 2
68. use the light source does not match the method Please exchange the light source or select another method The incorrect light source is installed Check that the intended light source s are correctly in place and that correct method is chosen Cannot start the exposure Flat frame calibration has not been performed Please run flat frame calibration for the selected settings Cannot start the exposure Flat frame calibration has not been performed for digitization epi illumination Please run flat frame calibration for digitization epi illumination Flat frame calibration data was not found There is no Flat Frame file that satisfies the exposure conditions Create a correction file that satisfies exposure conditions using the Flat Frame Calibra tion function in the Option menu Cannot start the exposure CCD cali bration has not been performed Please run CCD calibration for the selected exposure time Calibrate now and continue CCD calibration is not ready for exposure Click the OK button to start ex posure CCD calibration for the exposure conditions is initiated Cannot start the exposure CCD cali bration has not been performed Please run CCD calibration for the selected exposure time The CCD is not calibrated Create a correction file that satisfies exposure conditions using the CCD Calibration function in the Option menu Cannot start the exposure Automatic exposure r
69. way Only install driver software obtained from your manufacturer s website or disc Unsigned software from other sources may harm your computer or steal information v See details A successful installation message appears Click the Close button 3 4 2 3 Installation of ImageQuant LAS 4000 Control Software Windows VISTA 1 2 3 4 Insert the ImageQuant LAS 4000 Control Software CD Locate and double click the file ImageQuant LAS 4000 msi In the ImageQuant LAS 4000 InstallShield Wizard dialog click the Next button Read the license text If the license agreement is not acceptable please contact a GE Healthcare representative see the back cover of this manual for contact information Select I accept the terms in the license agreement and click the Next button Select destination folder in the dialog Jl ImageQuant LAS 4000 InstallShield Wizard i Destination Folder Click Next to install to this Folder or click Change to install to a different Folder f Install ImageQuant LAS 4000 to C Program Files i Change nstallShield lt Back Lez Cancel Click the Next button to install the software at the default folder C Program Files Click the Change button to install to a different folder Click the Install button If User Account Control UAC is enabled in Windows Vista a dialog displays the message An unidentified programs wants access to your computer Click Allow Click the F

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