Leica M-Series Stereo
Contents
1. 3282 Additional dimensional diagrams involving Leica ErgoModules are available from your Leica agency 24 Y nU ig Pa eiea M Z6 V A J 1005 Leica VLO Dimen with transmitted light stand with incident light stand 194 gt CO 1 0 Achromat P 1 0x Achromat 7 138 70 N gt p 76 2 85 1989 408 376 314 802 328 7 Additional dimensional diagrams involving Leica ErgoModules are available from your Leica agency 45 F A A y a JA ux m i 03 C 4A IA C AIAN VI 7 101 100915 m E7 8 4 86 With incident light stand with transmitted light stand 216 o 1 0x Plan 228 2 Additional dimensional diagrams involving Leica ErgoModules2. The dioptric adjustment and setting for parfocality when a graticule is in the eyepiece are described in the user manuals for photomicrographic and measuring systems 20 Stands Transmitted light stand bright field 20W AN P Fit the side faced column to the transmitted light base as described on page 16 Follow the safety instructions given on page 4 gt Fit the microscope carrier and the stereomicroscope as described on page 16 Fitting the bulb Never touch the bulb when it is hot AN You may sustain burns Do not touch the 6V 20W halogen bulb directly with your fingers because fingermarks can cause it to crack when heated Remove fingermarks immediately with a cloth dipped In pure alcohol P Insert the slider together with the bulb socket P Hold the new bulb with a cloth and insert it fully into the mount P Push the slider carefully but completely back along the rail to the stop Filters e The transmitted light stand contains a KG1 filter e You can insert other 50mm diameter filters if you need them P Pull out the slider P Insert the 50mm diameter filter gt Push the slider carefully but completely back along the rail to the stop Starting up The ex factory setting of the voltage selector is 230 for voltages between 200 240V and the fuse holder contains two 160mAT fuses gt Set the voltage selector to 115 239 P Fit the enclosed two 315mAT fu
3. Working distance 81 Plan 55 Planapo 19 Planapo 97 Planapo 135 Plan 297 Achromat 187 Achromat 49 Achromat Eyepiece 10x 21B 89 Achromat 27 Achromat 112 Achromat 149 Achromat 112 Plan 16x 14B 25x 9 5B Magnification changer position Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm MS5 Positions 0 63 1 0 1 6 2 5 4 When using the Plan and Planapo objectives MZ125 the magnification is increased by the factor 1 25x 41 Optical data Leica MZ75 MZ95 Obieciive 1 0x Plan 1 0x Planapo 1 6x Planapo 0 63x Planapo 0 5 Plan 0 32x Achromat 0 5x Achromat 1 5x Achromat 1 0 Achromat 2 0 Achromat 0 8 Achromat 0 63 Achromat Working distance Eyepiece 81 Plan 19 Planapo 97 Planapo 135 Plan 297 Achromat 187 Achromat 49 Achromat 89 Achromat 2 Achromat 112 149 Achromat Position 6 0 for MZ95 only When using the Plan and Planapo objectives MZ125 the magnification is increased by the factor 1 25x 42 Magnification changer position Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm
4. Leica M Series Stereo microscopes User Manual Seica MICROSYSTEMS Contents Page Overview CODE BDE vo ac que grec VU En oc das aper beatae 4 nn 5 Controls and ai ao Pau 29937 Pek nest ones 6 Short ceu x edicion pert SER ub P Ruben erro Y ex bate Rd 8 Use BIGONOMICS TRES E eu PEDE TES 9 Viewing height and interpupillary distance 9 Eyepoint eye COMLACL diim SEX Ta ba TOR 9 Changing the magnification 10 Ses E E E era ater oe 11 Stereoscopic and axial observation 12 ODE he SOUS SERERE PRICE dE E SCR gh ROIG doom ee eer 12 Working with the stereomicroscope 14 Assembly Basic outfit Overview Components 15 SDSPBI TIUS gos satu AS USUS qw PNE E TS 16 Fitting the column to the incident and transmitted light bases 18 MerOSCODO POI TIBI ca eios ro Tr PH toa EUER E ERR EE E E EE RT NM 18 Binocular tubes optics carrier optical accessories 19 Upndrqe 20 Stands Transmitted light stand
5. Retighten the clamping screw En Altering the overhang v P Release the clamping screw gt Displace the horizontal arm v Retighten the clamping screw 23 Assembly Large swinging arm and table clamp stands gt Table clamp stand Use the clamp to secure the column to a table top 20mm 50mm thick gt Swinging arm stand Introduce 4 Allen screws into the base from beneath and secure them to the end of the column Safety ring gt column The safety ring secures the instrument and must always be positioned beneath the horizontal arm gt Position the safety ring with the screw head uppermost and towards you and slide it down the column gt Tighten the clamping screw Safety ring gt column gt Lower the horizontal arm on to the safety ring with its end pointing towards you gt Tighten the clamping screw n n Limitation m If the horizontal arm is swung out beyond the limit the outfit may fall over The screw head must engage the notch It limits the lateral move ment It may not be removed and neither may the safety ring be milled down Drive housing gt horizontal arm Here follow directions for fitting the drive with side faced carrier rod To fit the drive housing with peg refer to page 22 P Release the clamping lever P Tilt the carrier rod downwards P Retighten the clamping lever P Slide the drive housing with s
6. Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Total magnification Field diameter mm Optical data Leica MZ125 MZAPO oes 1 0x Plan 0 5x Plan Objective 1 0x Planapo Position 10 0 for MZ125 only 60 Plan 55 Planapo Magnification changer position Total magnification Field diameter mm Total magnification Field diameter mm 0 63x Planapo Working distance 97 Planapo Total magnification Field diameter mm 0 8x Plan Total magnification Field diameter mm 1 6x Planapo 19 Planapo Total magnification Field diameter mm 43 Th A Y Em via el 4 6 W I F Ul J a 1 j B e e VIMENSIONS 1 yeD with incident light stand 1 0x Achromat C ES 4 y 4 1989 L SEEN MS5 NM Ea D in C o Y Y 70 120 A Y 320 at p with transmitted light stand 180 LF A 2 1 0 Achromat 138 bo Pod 198 9 R88 MH
7. are available from your Leica agency 46 aH ii LY E With incident light stand with transmitted light stand 216 247 118 426 O29 528 2 Additional dimensional diagrams involving Leica ErgoModules are available from your Leica agency 4 Te o TY o MAZI Aa EN WEG Y eJ IIMeNSTONS eleg VI Z7 PE e CICA 87410 with large incident light stand with transmitted light stand 216 Additional dimensional diagrams involving Leica ErgoModules are available from your Leica agency 48 With incident light stand with transmitted light stand 216 216 _ ww gt o 1 0x Planapo po E a CY E MZAPO 2 5 i S p 09 ES LO LO Y ee UUN 22 120 22749 90040 Additional dimensional diagrams involving Leica ErgoModules are available from your Leica agency Leica Microsystems the brand for outstanding
8. page 33 Adjust the eyecups page 9 Set both eyepieces to zero Set the microscope carrier AX for stereoscopic observation Hattest object Place such an object beneath the objective Lowest magnification Engage the lowest magnification position page 10 Use the coarse focusing drive to set the working distance page 11 Look into the eyepieces Using the focusing drive bring the test object into focus Highest magnification Engage the highest magnifica tion position Image sharpness Optimize with focusing drive C Adjust the dioptric settings 6 I Lowest magnification Engage the lowest magnification position Do not look into the eyepieces Rotate the eyepieces anticlock wise in the direction as far as the stop 5 diopters Look into the eyepieces Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged Highest magnification Engage the highest magnifica tion position Refocus Refocus if necessary Magnification changer Slowly move the magnification changer from the lowest magnification to the highest The image sharpness must remain constant parfocal Otherwise repeat the procedure 13 Use Working with the stereomicroscope In this section we will show you how to work smoothly with your stereomicro scope and how to study objects routinely Preconditions for working c
9. against a bright background au P To obtain bright field illumina tion push the knob fully in Transmitted light dark field e Inthe dark field technique the light from the source meets the object very obliquely from below Only rays which are reflected or refracted at interfaces reach the objective so edges and structural elements appear bright on a dark background even if the contrast of the original object was extremely poor 22 gt obtain dark field illumination pull the knob fully out Swinging arm stand ESD The swinging arm stand ESD is of conducting material surface resistance 10 Ohm square discharge time lt 2 seconds from 1000V to 100V Two sockets for connecting 4mm diameter ground cables are provided on the base Assembly Swinging arm stand with foot P Introduce 4 Allen screws into the base from beneath and secure them to the end of the column lt Safety ring gt column The safety ring secures the instrument and must always fixed beneath the cross member P Slide the safety ring down the column P Tighten its clamping screw Cross member column gt Lower the cross member on to the safety ring P Tighten the clamping screw Horizontal arm cross member lt gt Slide the horizontal arm into the cross member with the connecting piece towards you gt Tighten the clamping screw Drive housing gt horizontal arm
10. bright field 20W 20 Transmitted light stands bright and dark field 22 Swinging arm stand ESD 22 Large swinging arm and table clamp 5 5 24 26 L2 HE SOU TUB ES S 27 Fibre optic light guide 28 Cold light source Leica CLS Series 28 Mains line lamp 25W 28 Incident lamp 6V 10W 28 Tier eS nm 30 b ogxralb d 3 ore Sages oes bees 32 Near vertical 32 Accessories Fitting 1 33 Double iris 33 Drawing TD qoare S xoa 4 ue UE QE RE es 33 Video phototubes Photography video TV filming digital imaging 34 Attachment for vertical and oblique 5 37 Stages polarization 37 Special notes T
11. e he drive housing microscope carrier can be fitted to the horizontal arm in either of two positions see page 12 e The circular port in the microscope carrier accepts an illuminator gt Insert the peg of the inclinable focusing drive into the connecting piece gt Tighten the clamping screw Optics carrier gt microscope carrier P Fit the optics carrier in the microscope carrier gt Turn the optics carrier sideways in the microscope carrier as required gt Tighten the clamping screw Focusing The focusing knobs are located on the inclinable drive housing Is the focus movement too loose or too tight Does the outfit tend to slide downwards You can adjust the movement in accordance with the weight of the outfit and with your own personal requirements P Hold the drive knobs with both hands and turn them in opposite directions until the ease of movement is satisfactory Possibilities for adjustment Tilting P Release the clamping screw P Tilt the instrument gt Retighten the clamping screw SU ON 3 Altering the working distance P Release the clamping screw 2 P Raise or lower the cross member as required together with the horizontal arm i gt Retighten the clamping screw Reposition the safety ring see page 22 Sideways movement P Release the clamping screw B p Move the instrument sideways on the cross member yi P
12. For Leica MZ75 MZ95 MZ125 MZAPO P First screw the spacer ring s page 16 on to the microscope carrier then screw the adapter ring to the spacer ring gt Secure the incident light housing to the adapter ring gt Screw the achromatic objective into position see left side P Release the left or right clamping screw and change the cover plate accordingly P Attach the fibre optic light guide on either the left or the right To switch on the cold light illuminator refer to the user manual provided with it P Turn the rotary knob e A movable prism directs the light from the left or right outlet on to the object Accessories Double iris diaphragm drawing tube discussion tube Fitting e All accessory tubes which fit between the optics carrier and the binocular tube are fitted in the same manner e nthe dovetail ring of the optics carrier is a locating screw which must engage the notch in the accessory page 19 This is best accomplished by moving the accessory slightly from side to side P Release the clamping screw gt Introduce the accessory tube into the dovetail ring in the same manner as the binocular tube page 19 and move it slightly from side to side until the locating screw engages the notch gt Tighten the clamping screw P Fit the binocular tube on the accessory tube in the same manner Double iris diaphragm The double iris diaphragm can be fitted betwe
13. Regulating transformer The 0 7V 40VA transformer powers the 6V 10W and 6V 20W lamps Voltage selector Connections for two lamps maximum combined loading 40W CO Connection for power cable Fuse holder F1 0A Power switch A on O off 6 ON display Instrument is switched on 7 Regulating knob 8 6V display The maximum voltage has been reached Operation P Setthe available voltage on the voltage selector Position 115 for 110V 120V Position 230 for 200V 240V gt Connect the power cable to the socket and to the power supply gt Connect the lamp The sockets accept two lamps with a maximum combined loading of 40W 26 Switching on and off You can prolong the life of your bulb if you gt first switch off the lamp by turning the rotary knob into the black range gt and only then switch the transformer on or off gt Turn on the power switch The diode shines T b P Use the rotary knob to adjust the brightness Above 6V the diode shines Changing fuses Fuses 1 0A 5x20 stock no 167 651 gt Lamp off gt Power switch off P Turn the fuse holder anticlock wise and extract it P Fita new fuse and replace the fuse holder In the event of overload Lamp off Power switch off Wait a few seconds Power switch on vvvvyv Lamp on Step transformer A Follow the safety instructions given on page 4 e You can a
14. carrier Turn optics carrier sideways stereoscopic axial observation Microscope carrier for stereoscopic observation This microscope carrier permits only stereoscopic observation using both beam paths e he optics carrier can be turned sideways in the microscope carrier if the user needs to work from the side P Release the clamping screw gt Turn the optics carrier sideways as required gt Retighten the clamping screw The microscope carrier can be attached to the focusing drive in either of two positions e For short working distances and for flat objects use Position 1 Use this position also in con junction with the attachment for vertical and oblique observation see detailed instructions e Use Position 2 for long working distances and for bulky objects For assembly refer to page 18 12 Microscope carrier AX The stereomicroscopes can be equipped with the microscope carrier AX for stereoscopic and axial observation instead of the standard microscope carrier e he microscope carrier cannot be turned sideways when in the microscope carrier AX see page 24 e The microscope carrier AX can only be fitted in one position to the focusing drive see page 18 For the Leica MZ125 and Leica MZAPO use the spacer ring page 16 Stereoscopic observation A three dimensional image is essential for studying and working on spatial objects gt Shift the stereomicroscope to the central pos
15. cause damage to the equipment Power cables Inspect cables regularly for damage Defective cables can directly endanger personnel cause the stereomicroscopes and accessories to become electrically live endangering personnel Positioning cables Position cables with care Make sure that personnel do not get entangled with them The instrument could fall over fall down become damaged damage other equipment or cause personal Injury Opening the instrument Only authorized Leica personnel are permitted to repair electrical equipment Before opening up the equipment pull out the power cable If you touch the live interior of the equipment you may receive an electric shock Grid voltage Ensure that the voltage setting on the equipment is correct A wrong setting can cause damage to the equipment Connections When connecting power consuming devices to the trans formers do not exceed the maximum permitted loading as this can cause damage to the equipment Changing bulbs e Before changing a bulb disconnect the power cable e Never attempt to touch the inside of a lamp housing or transmitted light stand while the instrument is connected to the power supply e Before changing a bulb wait until it is cool otherwise you may burn your fingers Legal requirements Adhere to general and local regulations relating to accident prevention and environmental protection Conformity with European Community d
16. magnification position P Use the focusing drive to refocus Now the object should remain in focus at all zoom magnifications Otherwise check that your dioptric settings have been adjusted exactly as described on page 12 Basic outfit The components 1a Incident light base with stage plate 1b Transmitted light base with glass stage plate 2a Side faced column with drive housing coarse fine 2b Side faced column with drive housing Microscope carrier Optics carrier Interchangeable objective Binocular tube Wide field eyepiece for spectacle wearers with eyecups C Ci A Various ErgoModules or accessories for video photography drawing fluorescence coaxial incident light etc Spacer rings Spacer rings are required in order to attach the objectives for certain instrument configurations e The Leica MZ95 is supplied with a spacer ring for the smaller diameter achromatic objectives and 1 0x plan achromatic objective When the spacer ring has been removed by applying the tool provided the larger diameter planachromatic and planapochromatic objectives for the MZ125 MZAPO can be used e The Leica MS5 MZ6 and MZ75s can also be equipped with the larger diameter planachromatic and planapochromatic objectives for the MZ125 MZAPO if a spacer ring is fitted When the MZ125 MZAPO planachromatic and planapo chromatic objectives are used on the Leica MS5 MZ6 MZ75 and MZ95 stereomicrosc
17. pages indicated MZAPO Inspect the cleanliness gt Remove dust and dirt from the instrument page 39 gt Clean Optical accessories glass stage plates and any graticules used page 39 Only for variable ErgoModules gt Adjust viewing height page 9 Only for photomicrographic outfits gt Lever at position VIS P Fortrinocular tube Push diaphragm lever to the right gt Open double iris diaphragm position OPEN Adjust the interpupillary distance gt Using both hands displace the eyepiece tubes page 9 Select the degree of eye contact gt Secure the eyecup in the position required page 9 Adjust the dioptric setting P Adjust the dioptric setting precisely for each eyepiece page 12 Set the magnification required gt Turn the rotary knob to the desired magnification page 10 Focusing gt Look into the eyepieces and focus on the object page 11 e Only for coarse fine drive Focus approximately with the large knob and then accurately with the small knob gt Ifthe focusing movement is too loose or too tight adjust the ease of movement page 11 Adjust the depth of field gt Adjust the depth of field with the double iris diaphragm page 33 Microscope carrier AX gt For measuring photography and polarization engage the vertical beam path page 12 Use Ergonomics viewing height interpupillary distance eye contact Ergonomics The reasons w
18. 09 34 34 Fax 41 1 809 34 44 United Kingdom Milton Keynes Tel 44 1908 666 663 Fax 44 1908 609 992 USA Bannockburn lllinois Tel 1 800 248 0123 Fax 1 847 405 0164 and representatives of Leica Microsystems in more than 100 countries The Business Units in Leica Microsystems hold the management system certificates for the international standards ISO 9001 and ISO 14001 relating to quality management quality assurance and environmental management Leica Microsystems Ltd Business Unit SM CH 9435 Heerbrugg Switzerland Telephone 41 71 727 31 31 Fax 41 71 727 46 76 www leica microsystems com The companies of the Leica Microsystems Group operate internationally in five business segments where we rank with the market leaders Microscopy Our expertise in microscopy is the basis for all our solutions for visualization measurement and analysis of microstructures in life sciences and industry Specimen Preparation We specialize in supplying complete solutions for histology and cytopathology Imaging Systems With confocal laser technology and image analysis systems we provide three dimensional viewing facilities and offer new solutions for cytogenetics pathology and material sciences Medical Equipment Innovative technologies in our surgical micro scopes offer new therapeutic approaches in microsurgery With automated instruments for ophthalmology we enable new diagnostic methods to be applied Semiconductor Equipm
19. MZAPO Press the switch gt For MZ7s Turn the knurled ring anticlockwise 10 Scale of magnifications MS5 MZ6 Display of magnification factors from 0 63 4 MZ75 Display of magnification factors from 0 63 5 For other objective eyepiece combinations appropriate adhesive scales can be applied to the rotary knobs page 19 MZ95 Display of magnification factors from 0 63 6 2125 Display of magnification factors from 0 8 10 MZAPO Display of magnifications with various optical combinations e g with 1 0x objective and 10x eyepieces 8x 80x MZAPO Setting the scale The magnification scale can be set in accordance with the optical combination Example 1 0x objective 25x eyepiece gt Turn the magnification changer to the stop at its lowest magnifi cation position gt Read the magnification range from the table on page 43 in the example given 20x to 200x P Place a finger in the rear window of the magnification changer and displace the scale so that the position 20 Is lined up with the index Magnifications and fields of view The tables between page 41 and page 43 provide information about the magnifications and field diameters as a function of the magnification changer position of the eyepiece objective combination used Magnification with MZ125 MZAPO Pan and Planapo objectives When the MZ125 MZAPO planachromatic and planapochromatic objectives are u
20. Z75 MZ95 MZ125 the binocular tube ErgoTubus ErgoKeil M 5 25 the swinging arm stand ESD and the cold light sources Leica L2 and CLS consist of ESD discharging material surface resistance 10 ohms squared discharge time 2 seconds 1 000V to 100V e Aconnection socket for a 4mm dia earthing cable is provided on the microscope carrier here are two connection sockets for 4mm dia earthing cables in the base of the swinging arm stand Servicing Repairs may only be carried out by Leica trained service technicians Only original Leica spare parts may be used Responsibilities of person in charge of instrument e Ensure that personnel who use the instrument have read and understood this user manual and in particular the safety Instructions e Ensure that the Leica MS5 MZ6 MZ75 MZ95 MZ125 and MZAPO stereomicroscopes are operated maintained and repaired only by authorized and trained personnel safety regulations Electrically operated items The safety regulations listed below apply to the following items Transmitted light stand bright field 20W page 20 Mains lamp 25W page 29 Regulating transformer 0 7V 40W page 26 Step transformer 4 5 6V page 26 Motor focus separate instructions Liquids Be careful when handling liquids If spilt on the equipment they can cause the stereomicroscopes and accessories to become electrically live endangering personnel
21. bath The polymer can become brittle and later break e Never use solvents for cleaning except for ethanol and isopropanol You can clean polymers harmlessly e with warm soapy water wiping down afterwards with distilled water e with ethanol industrial alcohol and isopropanol When cleaning with ethanol or isopropanol adhere to the appropriate safety regulations 39 Notes Total magnification and field diameter Mg Magnification of objective Magnification of eyepiece 7 Magnification changer position q Tube factor e g 1 5x for coaxial incident light 45 Ergotube 1 6x r Factor 1 25x if the planachromatic and planapochromatic objectives of the MZ125 MZAPO are used on the 55 MZ6 275 or MZ95 Field number of eyepiece The field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Example Mg Eyepiece 1 0x 25x 9 5 eyepiece Z Zoom position 4 0 q Tube factor 1 5x because of coaxial illuminator r Factor 1 25x Magnification down binocular tube Mrotvig Mexzxqxr 1 0 25 x 40 1 5 1 25 1875 Diameter of field of view Nroy 9 5 f gp 13mm Mgxzxqxr 1 0 x 40 x 15 x 125 40 Optical data Leica MS5 MZ6 1 0 x Plan 1 0xPlanapo 1 6xPlanapo 0 63 x Planapo 0 5 x Plan 0 32 x Achromat 0 5 x Achromat 1 5 x Achromat Objective 1 0 x Achromat 2 0 x Achromat 0 8 x Achromat 0 63 x Achromat 0 8 x Plan
22. ce with the instruc tions gt Position the photo outlet on eit her the right or the left Switchover P Observation Push lever to VIS gt Documentation Set lever to PHOT Light partition in setting VIS e 100 to both eyepieces Light partition in setting PHOT e 50 to both eyepieces e 50 available for video photo Video phototube A This tube does not permit stereoscopic observation The image Is studied either in the camera viewfinder or down the focusing telescope of the camera In the video photo beam path 100 light is available Attachment for vertical and oblique observation e The attachment for vertical and oblique observation enables spatial objects to be observed from above and also from all round at an angle of 45 e he microscope carrier must be fitted in the lower position 1 page 18 For MS5 MZ6 Fit the 1 0x achromatic objective by screwing Its adapter ring directly into the optics carrier e Spacer rings are required for the Leica MZ75 MZ95 MZ125 and MZAPO in order to secure the attachment for vertical and oblique observation to the optics carrier page 16 e Only usable with 1 0x achromatic objective Stages The 120mm diam stages can be used in those incident and transmitted light stands which have a 120mm diam port Gliding stage The gliding stage can be moved in all directions and rotated completely gt Place the 120mm diam gli
23. ched correctly e Are all connecting cables attached correctly e Are the fuses in order The image is too dark Remedy e Turn up the regulating knob high enough bright field transmitted light stand page 21 transformer page 26 e Open the double iris diaphragm of the phototube TV photography The image on the screen is too dark Remedy e Adjust the video camera monitor system in accordance with the directions of the manufacturer e Refer to the remedies listed above under The image 15 too dark 38 Unsharp photographs Remedy e Focus precisely page 11 e Adjust your dioptric settings exactly as described page 12 Colour photographs have a yellow cast Remedy e Use artificial light film e Turn the regulating knob of the transformer clockwise to the position of maximum voltage bright field transmitted light stand page 21 transformer page 26 Care In this section we will show you how to look after your microscope and how to clean it We guarantee the quality You are working with a high precision high performance instrument We therefore guarantee the quality of each of our instruments Our guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Please handle your valuable optical instrument with respect It will then fulfil its function with the same precision for decades Our instru
24. ding stage in the 120mm port and secure it with the eccentric lever gt Insert the 120mm diam glass stage plate or the black white stage plate Cup stage e The cup stage can be inclined as required e You can secure objects to its rubberized upper surface with needles e The movable holder accepts petri dishes gt Place the 120mm diam support ring in the 120mm port P Place the cup stage on it Polarization transmitted light The analyzer in combination with either the rotatable polarization stage or the polarizer on glass stage plate enables double refracting materials to be studied These can include organic crystals mineral grains thin sections of rock or bone polymers stressed glass and liquid crystals 37 Notes Tips and hints What to do if The field of view is partly shadowed Remedy e Set the interpupillary distance correctly page 9 e Locate the eyepoint page 9 The image does not stay in focus Remedy e Fit the eyepieces correctly page 19 e Adjust your dioptric settings exactly as described page 12 The focusing drifts downwards or can be moved only with difficulty Remedy e Adjust the ease of movement pages 11 22 24 If an electrical fault develops first check the following e 5 the voltage selector set correctly bright field transmitted light stand page 21 transformer page 26 e sthe power switch on e Is the power cable atta
25. ece is about 22mm e When you can see the entire field of view without restriction you have found the correct position eyepoint gt find the eyepoint slowly bring your eyes closer towards the eyepieces Selecting the degree of eye contact If you do not wear spectacles and want closer contact to the eyepieces Holding the diopter ring turn the eyecup anticlockwise until it is released Pull the eyecup upwards Holding the diopter ring turn the eyecup clockwise Spectacle wearers need the whole of the available distance Move the eyecups to the lowest position Use Magnification changer 55 5 steps MZ6 6 1 zoom 475 7 9 1 zoom MZ95 9 5 1 zoom MZ125 12 5 1 zoom MZAPO 10 1 zoom Zoom means continuous change of magnification Ratchets can be engaged for certain magnifications see below Changing the magnification e he magnification changer can be operated from either the right or the left P Look into the eyepieces gt Focus on the object page 11 gt Turn the magnification changer to the position required Ratchet steps MZ6 0 8 1 0 1 25 1 6 2 2 5 3 2 MZ75 0 8 1 1 25 1 6 2 2 5 3 2 4 2 5 3 2 4 MZ9s 0 8 1 1 25 1 6 2 2 5 3 2 4 5 125 T 1 25 1 6 2 2 5 3 2 4 5 6 3 6 MZAPO 10 12 5 16 20 25 32 40 50 63 Engage the ratchets whenever magnifications need to be exactly reproducible 5 P ForMZ6 MZ95 MZ125 and
26. en the optics carrier and either the binocular tube or the video phototube Set the lever to OPEN The double iris diaphragm aperture diaphragm is open The depth of field increases as the lever is moved from OPEN to 1 Remember however that brightness decreases so exposure times become longer resolution deteriorates Drawing tube e The drawing tube is for both left handers and right handers Fit the mirror on the left or right hand side of the optics carrier gt Fit the drawing tube between the optics carrier and the binocular tube as described on the left of this page gt Place a sheet of paper beneath the mirror gt Use a table lamp to illuminate the paper Drawing P Move the lever to the circle gt Look into the binocular tube position the object and focus on it gt Move the lever to the black spot gt Look into the binocular tube and balance the brightnesses so that the object the drawing surface and the pencil are all seen together ngeneral the paper needs to be illuminated strongly but the object only weakly gt Move the lens mount of the drawing tube forwards backwards until the drawing surface is sharply imaged gt Now you can draw the feature e fyou place a graduated scale beneath the object and draw Its intervals as well you will easily determine the image scale on the paper Discussion tube Down the discussion tube two obser
27. ent Our automated leading edge measurement and inspection systems and our E beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world MICROSYSTEMS Illustrations descriptions and technical data are not binding and may be changed without notice Leica Microsystems Ltd CH 9435 Heerbrugg Switzerland 1998 Printed on chlorine free paper with a high content of recycled fibre Publication no in English M2 105 0en German M2 105 0de French M2 105 0fr e Spanish M2 105 0es Printed in Switzerland VII 2001 RDV
28. ent light 10 446 180 0 10446300 0 10 446 300 C Microscope carrier AX 10 445 618 10446 172 10446172 Near vertical illuminator 10 445 198 C C CA 2x 10 446 300 Attachment for vertical 10 445 156 2 10 446 300 10 446 300 10 446 300 10 446 300 um DI e mm C Compatible CA Compatible if used with intermediate ring order separately 0 Intermediate ring recommended M Magnification increased by factor 1 25x Remove intermediate ring 10 446 393 already included with 295 17 Assembly In the following sections we will explain how to assemble a complete stereomicroscope outfit including the incident and transmitted light stands Detailed directions about the transmitted light stands the swinging arm stand and the fitting of the remaining accessories are all described in the appropriate sections see contents list on page 3 Side faced column gt incident light base T gt Remove the stage plate P Introduce 3 Allen screws into the underside of the baseplate and secure them to the bottom end of the side faced column C P Insert the stage plate The side faced column with focusing drive Is available with column lengths 300mm and 500mm To fit the microscope carrier and the stereomicroscope see the next column of text Side faced column gt transmitted light base Transmitted light stand bright field 20W Pull the power cable ou
29. ert the edge of its dovetail ring beneath the two lugs on the optics carrier Move the binocular tube from side to side until the locating screw on the optics carrier engages the guide Tighten the clamping screw Insert a graticule if appropriate page 20 Release the clamping screws on the eyepieces Insert the eyepieces fully into the eyepiece tubes Check that they have seated correctly Tighten the clamping screw You can extend the overall magnification range by using 16x 25x and 40x wide eyepieces for spectacle wearers 19 Graticules The graticules for measuring and counting are in mounts which can be fitted directly into the eyepieces A highly precise stage micrometer with a 50mm scale and 0 1mm and 0 01mm divisions enables the graticule to be calibrated in accordance with the magnification selected It is best to measure with the microscope carrier AX in vertical position The measurements are parallax free and therefore more precise Focusing and framing graticules indicate the format limits for all films Fitting the graticule P Unscrew the sleeve from beneath the eyepiece gt Push the graticule in mount over the sleeve ensuring that It seats properly gt Screw the sleeve into position again gt Insert the eyepiece fully into the eyepiece tube gt Looking into the eyepiece turn the eyepiece in the eyepiece tube to align the graticule and tighten the clamping screw
30. hy users often cannot work comfortably with the 45 tube are the differing heights of the outfits the various accessories and working distances and the fact that the same instrument is often used by several people of differing builds This problem can be effectively solved by using the Leica ErgoModules If you have problems with the viewing configuration on your stereomicroscope ask your Leica consultant for the most ergonomic Leica solutions Viewing height viewing angle e The viewing height is correct when your head and back are comfortably positioned 50 Onthe ErgoTube the viewing angle is adjustable from 10 50 gt Holding the eyepiece tubes at their bases swing them up or down see arrow ErgoModule e ErgoWedge 5 25 e ErgoWedge 15 e ErgoModule 50mm e ErgoTube 45 has eyepiece tubes which are 65mm longer than the standard ones e ErgoModule 30mm 120mm Pi After several years regreasing may be necessary Send the ErgoModule to the Leica service workshop Interpupillary distance e The interpupillary distance is correct when you see a single circular field when viewing with both eyes 76 apt gt Look into the eyepieces and use both hands to move the eye piece tubes closer together or further apart Locating the eyepoint e For 10x 21B wide field eyepieces for spectacle wearers the distance from eye to eyepi
31. ication scale 55 5 steps 6 6 1 zoom 275 7 9 1 zoom MZ95 9 5 1 zoom MZ125 12 5 1 zoom MZAPO 10 1 zoom 2 Engaging ratchet positions MZ6 Switch engages 7 magnification steps MZ7s Milled ring engages 8 magnification steps MZ95 Switch engages 9 magnification steps MZ125 Switch engages 10 magnification steps MZAPO Switch engages 9 magnification steps Focusing drive 3b Coarse and fine focusing Larger knob Coarse focusing Smaller knob Fine focusing 3c Adjustable ring Changes the ease of movement of the coarse fine drive 4 Clamping screw Holds the optics carrier in the microscope carrier 5 Clamping screw Holds the binocular tube or accessory to the optics carrier 6 Interchangeable objective Various objectives achromatic planachromatic planapochromatic depending on the outfit Binocular tube Adjustable eyepiece tubes Interpupillary distance adjustable within the range 52mm 76mm 8 Wide field eyepieces for spectacle wearers Dioptric setting adjustable within the range from 5 to 5 and eyecups adjustable 9 Clamping screws These hold the eyepieces in the binocular tube 10 The viewing angle is adjustable within the range 10 50 Leica Design by Ernest Igl Christophe Apoth loz MS5 MZ6 MZ75 MZ95 MZ125 Short instructions Checklist In this overview we have summarized the working sequence You will find detailed user instructions on the
32. ide faced carrier rod on to the horizontal arm gt Tighten the clamping lever The clamping levers can be positioned as required P Pull out the clamping lever along Its axis and turn it Microscope carrier gt drive housing P Secure as described page 18 Optics carrier gt microscope carrier P Secure as described on page 18 Possibilities for adjustment Tilting Sideways movement Altering the overhang P Release the clamping lever P Tilt the instrument gt Retighten the clamping lever gt Set the distance approximately with the larger knob gt Fine focus with the smaller concentric knob gt Using the ring on the right hand drive knob adjust the ease of movement of the drive P Release the wing screw gt Raise or lower the horizontal arm gt Retighten the wing screw Reposition the safety ring see page 24 P Release the wing screw gt Pull out or push in the horizontal arm gt Retighten the wing screw During the sideways movement respect the end stops see page 24 P Release the wing screw gt Pull out or push in the horizontal arm gt Retighten the wing screw 25 Illluminators Transformers Follow the safety instructions given on page 4 Leica transformers may only be opened by authorized service personnel and the power cable must be disconnected beforehand The transformers are not to be used outdoors
33. il slide the fibre optic light source into the adapter fork until it engages 27 Illuminators Fibre optic light guide e he clamp of the fibre optic light guide cannot be attached to the microscope carrier AX gt Turn the clamping screw out of in the clamp gt Hold the clamp in the opening of the microscope carrier gt Push the clamping screw through the holes in the micro scope carrier and in the broader part of the clamp and screw it finger tight to the narrow part of the clamp P Insert the fibre optic light guide into the clamp gt Switch on the power unit gt Align the light spot with the object P Tighten the clamping screw Cold light source Leica CLS series The Leica CLS series is a high performance line for high light intensity within the smallest possible space and flicker free white light with the lowest possible thermal effect on the objects The comprehensive line of accessories enables unli mited use The CLS series is produced of antistatic material When using the ring lamp 76mm dia on the Plan objective 0 8x 80mm dia a special adapter 10 447 078 is required For a detailed information refer to the separate user manual Leica CLS Mains line lamp 25W Follow the safety instructions given on page 4 P Fit the lampholder and lamp as described in the right hand column incident lamp 6V 10W gt Connect the 25W mains line lamp to the powe
34. ips and hints What to do if 38 Care and maintenance of the stereomicroscope maintenance 39 Calculating total magnification and field diameter 40 Optical data MS5 6 saison dca oes eie e cde ede Di c hcic inc 41 Optical data MZ75 MZ95 42 Optical data WIC 125 43 DMMENSIONS eves eee oe IEEE 44 To you the user Thank you for your trust We wish you success and satisfaction in your work with your new stereomicroscope from Leica The innovative technology of the new stereomicroscope line from Leica is expressed externally in the avant garde form of the instrument The rounded shapes with their elliptical tendencies demonstrate that beauty can be practical In developing this new stereomicroscope line we have placed great weight on simple self explanatory directions We have however put a lot of effort into explaining all functions to you by means of pictures and text You will only get the best out of your stereomicroscope if you take the trouble to study this user manual in detail If you still have a question sometime just contact your local Leica agency or get in touch directly with Leica Microscopy Systems Ltd Heerbrugg Switzerland We are gladly at your service Customer service is a big thing wi
35. irective The electrically operated accessories for the Leica MS5 MZ6 MZ75 MZ95 MZ125 and MZAPO stereomicroscopes are constructed in accordance with the latest technologies and are provided with a statement of conformity with EC requirements You will find these symbols in the user manual Safety direction This symbol denotes information which must be read and obeyed Failure to respect it can cause injury Failure to respect it can cause malfunctioning or damage to the equipment Warning about accessible hot places e g bulbs 0 Failure to respect it can cause burns Important information This symbol denotes additional information I or explanations which promote understanding Action gt This symbol within the text indicates that certain operations must be carried out Explanatory notes e This symbol within the text stands for additional information and explanations Overview Standard outfit Controls and functions In this overview we explain the controls and functions relating to the standard outfits Does your stereomicroscope look different from the one in the picture The modular design enables you to assemble an outfit matched to the application Controls and functions which differ from those in the standard outfit are described in the appropriate section see contents list on page 2 Leica MZAPO see page 8 Stereomicroscope 1 Magnification changer Drive knobs with magnif
36. ition Axial observation The parallax free imaging produces more precise results in photography measuring and polarization p Shift the stereomicroscope to the left or the right position The object is now located directly beneath either the left or the right beam path e When photographing or measur ing select the beam path above which the phototube or measur ing graticule is located Adjusting the dioptric settings and the parfocality e You must adjust the dioptric settings for both eyes I exactly as described on page 13 Only then will the image be parfocal i e it will remain sharp whenever the magnification is changed and there will be no need to refocus Make the most of this opportunity which is not offered by every stereomicroscope e The dioptric range is from 15 to 5 The following adjustments must be carried out only once by each user The procedures for adjusting the dioptric setting and the parfocality are somewhat different if an eyepiece contains a graticule Refer to the user manuals for the MPS modular photomicrographic systems or for measuring A Preparations v Set up the illumination pages 20 21 26 32 P Setthe interpupillary distance and if necessary the viewing height page 9 gt On the video phototube Move the lever to position VIS and open the diaphragm B Focus on the test object m set the double iris diaphragm to OPEN
37. l of 4 filterholders with 50mm diameter filters can be attached Changing the bulb Disconnect the lamp cable from the transformer Never touch the bulb when it is hot AN You may sustain burns e You can separate the lamp housing and the bulb socket Line up the arrow head on the mount with its shaft on the housing Do not touch the 6V 10W halogen bulb because fingermarks can cause itto crack when heated Remove fingermarks with a cloth dipped in alcohol gt Turn the socket to the separation position gt Pull the socket forcibly out of the housing gt Hold the new bulb in a cloth and push it into the socket gt Replace the socket in the housing The socket clicks into position Operating the lamp AN Follow the safety instructions given on page 4 Follow the directions for using the transformer page 26 gt Connectthe cable between the lamp housing and the trans former page 26 gt Switch on the transformer and use the rotary knob to adjust the brightness page 26 Hold the lamp housing and release the clamping screw P Align the light spot with the object P Tighten the clamping screw gt Swing in the heat absorbing filter gt Enlarge or reduce the size of the light spot by turning the socket of the bulb 29 Illuminators 6V 20W incident lamp e The 6V 20W lamp is an inclined incident source for illumi nating spatial objects e By using two
38. lamps you can soften or eliminate shadows Atthe nominal voltage 6V the colour temperature is 3200K ideal for photography Set the regulating transformer to this nominal voltage see page 26 Assembly P Release the clamping screw gt Unfold the lampholder arm gt Secure the lampholder to the lamp P Use the screw to secure the clamp to the side faced column either above or below the drive housing P Using a fork wrench and the thread connector attach the lampholder arm to the clamp on either the right or the left gt Position the lamp gt Tighten the clamping screw e The thread connector also enables the lampholder to be connected to the cast foot 30 Diffusing and other filters e A total of 4 filterholders with 32mm diameter filters can be attached A heat absorbing filter is built in ss Push the filterholder complete 2 with diffusing filter on to one of the pegs and swing it in Operating the lamp AN Follow the safety instructions given on page 4 Follow the directions for using the transformer page 26 gt Connect the cable between the lamp housing and the trans former page 26 gt Switch on the transformer and use the rotary knob to adjust the brightness P Push the condenser lens forwards or backwards until the slider engages gt Hold the lamp housing and release the clamping screw P Align the light spot with
39. low and is seen in its natural colours against a bright background gt For transmitted light bright field 7 Pull lever forwards Inclined transmitted light e Inclined transmitted light which traverses the object obliquely will produce effects advantageous for observing small translucent objects such as foraminifera and fish eggs L Transmitted light stand HL gt Move the lever slowly towards the column of the stand until the desired effect is obtained Detailed information about the assembly and use of this stand is given in the separate user manual M2 296 004 supplied with it 21 Assembly Transmitted light stand for bright and dark field The illuminator of the transmitted light stand for bright and dark field consists of a fibre optic light guide and a light source Read and respect the safety directions provided by the manufacturer of the light source P Fit the side faced column to the transmitted light base as described on page 18 gt Fit the microscope carrier and the stereomicroscope as described on page 18 gt Connect the fibre optic light guide to the stand and to the light source Starting up Before using the light source read the user manual for it Transmitted light bright field e Bright field is suitable for examining translucent objects having contrasting structures The object is directly Illuminated from below and is seen in its natural colours
40. ments are famous for that If contrary to expectations your stereomicroscope develops a fault please call in a specialist contact your local Leica agent or Leica Microsystems Ltd Heerbrugg Switzerland NS gt Protect your instruments from damp vapours acids alkalis and corrosive sub stances and keep chemicals away from the instruments e from misuse Never dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manual e from oil and grease Do not grease guide surfaces N or mechanical parts Dust and dirt will affect the quality of your results Therefore gt Puta dust cover over the instru ment during breaks in work gt Use dust caps to protect tube openings tubes without eyepieces and eyepieces gt Remove dust with a pneumatic rubber bulb or with a soft brush gt Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol gt When accessories are not being used protect them against dust Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuitable cleaning agents and techniques can damage polymers so always observe the following rules Never clean polymers this way AC e Never use corrosive cleaning agents or ones containing acetone Never clean in an ultrasonic
41. minator e This illuminator is for observing and photographing flat highly reflecting objects such as wafers integrated circuits and polished metal sections e The coaxial incident light housing fits like an accessory tube between the optics carrier and the binocular tube or between the optics carrier and the phototube page 33 e For the MZ75 and MZ95 it is an advantage to attach the objective to the spacer ring page 16 You will then be able to illuminate the largest field of view completely e A quarter wave plate is required when the microscope carrier AX is used in the vertical position gt Using the clamping screw secure the quarter wave plate to the objective mount gt Using the milled ring turn the quarter wave plate until the required effect is obtained Near vertical illuminator e The near vertical approx 5 light beam illuminates depressions and cavities e Shadowing by tools is eliminated e This illuminator can only be used with the achromatic objectives having focal lengths 100mm 150mm 175mm and 200mm e Spacer rings are required for the Leica MZ75 MZ95 MZ125 and MZAPO in order to secure the near vertical illuminator to the optics carrier page 16 gt Screw off the objective P Release the clamping screw and remove the adapter ring from the incident light housing for near vertical light For MS5 MZ6 gt Screw the adapter ring on to the microscope carrier 32
42. n 1 see above e The optics carrier cannot be turned sideways in the microscope carrier AX e The Leica MZ12s and Leica MZAPO require a spacer ring page 16 in order to secure the planachromatic and planapochromatic objectives to the microscope carrier AX Optics carrier gt microscope carrier gt Gently lower the optics carrier into the microscope carrier gt Use the screw to secure the optics carrier in the position required Apply the adhesive scale of magnifications Only for MZ75s P Remove the screws gt Remove the rotary knobs gt Apply the adhesive scales gt Replace the rotary knobs gt Screw on the selected objective anticlockwise e Some instrument configurations require spacer rings in order to secure the objectives page 16 These rings are fitted and removed using the tool provided e The Leica MZ95 is supplied with a spacer ring for the achromatic objectives and for the 1 0x planachromatic objective When the ring has been removed by means of the tool provided the larger diameter planachromatic and planapochromatic objectives for the MZ125 MZAPO can be fitted Binocular tube P Release the clamping screw If you have an accessory such as an ErgoModule page 9 a coaxial illuminator page 32 a video phototube page 35 or a filter slide holder page 35 fit it now to the optics carrier Tighten the clamping screw Tilt the binocular tube and ins
43. ng photographs engage the obser vation beam path e Accessories such as the coaxial incident illuminator always fit between the stereomicroscope and the phototube Video phototube HD gt Use the video phototube HD between the optics carrier and the binocular tube page 33 Switchover P Observation Push lever in gt Documentation Pull lever out Light partition for observation e 50 to both eyepieces e 50 in the left hand video photo beam path e 50 in the right hand video photo beam path Light partition for documentation e 100 to left video photo beam path e 50 to right eyepiece e 50 to right video beam path Camera set up variants As shown in the set up diagram on page 34 there are various possibilities for adapting MPS video digital or SLR camera Example as gt Fit the video photo objective H on the left hand side With this equipment you can for example attach an MPS camera see detailed description in the MPS user manual an SLR camera or even a digital or video camera Example With this equipment you can for example attach a digital or video camera P Fit the video objective 2 on a Leica DC digital camera 3 with the C mount connection gt Connect the video objective with the mounted Leica DC digital Camera on the right output of the 3 video phototube gt Tighten the coupling ring 1 Trinocular video phototube 50 gt Fit the t
44. omfortably Pay great attention when setting up your stereomicroscope You will only benefit fully from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given For fatigue free work use the ErgoModules page 9 Arrange your workplace as well as possible and pay attention to the heights of chair and table When sitting use the entire seat area and also the back support of the chair Support your forearms During work breaks stand up and move around for exercise Tips for your work The stereomicroscopes parfocally matched This 14 means that once you have focused on a feature in the manner described taking account of dioptric settings page 12 you can zoom the magnification up and down without refocusing You only need to refocus if you want to look at higher or lower planes in the object When focusing on a fresh object always start with the lowest magnification because the larger field of view makes the object easier to find Afterwards select the magnification you want P Place the object beneath the objective gt Illuminate the object gt Select the lowest magnification position P Using the focusing drive set the working distance approximately page 11 P Look into the eyepieces and use the focusing drive to bring the object precisely into focus P Select the highest
45. opes the magnification is increased by a factor of 1 25x 10 445 821 MZAPO 10 446 370 2125 10 446 272 MZ9 10 446 371 275 10 445 614 26 10 445 613 55 10 445 819 1x Plan 10 446 275 1x 10 446 157 0 5x 10 422 562 1 5x Plan 10 446 230 1x 10 446 172 10 422 561 2x 10 472 650 1 6x 10 473 832 0 8x 10 446 236 0 63x 10 445 201 0 63x Plan Planapo 10 411 589 1x 10 446 275 1x 10 422 563 0 5x 10 422 562 1 5x Plan 10 422 564 0 32x 10 422 561 2x 10 446 157 0 5x Achromat 10 473 832 0 8x 10 446 230 1x 10 445 201 0 63x 10 472 650 1 6x s 10 446 236 0 63 Si 10 447 075 0 8x gt Achromat ta E 10 367 929 E 10 315 306 10 445 352 E 10 367 929 10 315 306 10 445 352 16 Objective combinations Acomti5x mms e c c js d Achromat2x mme c c j o J Acomt0s C c o Achromatossx c j c j o Acomt05 mms e J c c Acoma03 ms C 4 omas c c Planapo 1x 2125 2 10446 230 CA 10446172 M CA 10446172 M CA 10 446 172 M C M Planapo 16x 2125 10472650 10446172 CA 10446172 M CA 10 446 172 M C M Planapo 0 63x 2125 2 10446236 10446 172 CA 10446172 M CA 10446172 M C M Accessories Pf Coaxial incid
46. products Leica Microsystems Mission is to be the world s first choice provider of innovative solutions to our customers needs for vision measurement lithography and analysis of microstructures Leica the leading brand for microscopes and scientific instruments has developed from five brand names all with a long tradition Wild Leitz Reichert Jung and Cambridge Instruments Leica symbolizes not only tradition but also innovation Leica Microsystems an international company with a strong network of customer services Australia Gladesville NSW Tel 41 800 625 286 Fax 61 2 9817 8358 Austria Vienna Tel 43 1 486 80500 Fax 43 1 486 80 50 30 Canada Richmond Hill Ontario Tel 1 905 762 20 00 Fax 1 905 762 89 37 China Hong Kong Tel 8522 564 6699 Fax 8522 564 4163 Denmark Herlev Tel 445 44 5401 01 Fax 445 44 5401 11 France Rueil Malmaison C dex Tel 33 1 4732 8585 Fax 33 1 4732 8586 Germany Bensheim Tel 449 6251 1360 Fax 449 6251 136 155 Italy Milan Tel 39 02 57 486 1 Fax 439 02 5740 3273 Japan Tokyo Tel 813 543 596 09 Fax 813 543 596 15 Korea Seoul Tel 82 2 514 6543 Fax 482 2 514 6548 Netherlands Rijswijk Tel 31 70 41 32 130 Fax 31 70 41 32 109 Portugal Lisbon Tel 35 1 213 889 112 Fax 35 1 213 854 668 Singapore Tel 65 77 97 823 Fax 65 77 30 628 Spain Barcelona Tel 34 93 4949530 Fax 34 93 494 9532 Sweden Sollentuna Tel 46 8 625 45 45 Fax 46 8 625 45 10 Switzerland Glattbrugg Tel 41 1 8
47. r supply 20 gt Switch on the mains line lamp P Align the light spot with the object as described above 6V 10W incident lamp he 6V 10W lamp is an inclined incident source for illuminating spatial objects e By using two lamps you can soften or eliminate shadows e Atthe nominal voltage 6V the colour temperature is 2700K Assembly P Release the clamping screw gt Unfold the lampholder arm P Tighten the clamping screw Lampholder clamp for side faced columns P Use the screw to secure the clamp to the side faced column either above or below the drive housing gt Use the connector to attach the lampholder arm to the clamp on either the right or the left Lampholder gt cast foot P Fitthe 25mm diameter adapter to the cast foot gt Using the connector attach the lampholder to the adapter Lampholder gt objective gt Remove the objective gt Slide the adapter over the objective gt Using the connector attach the lampholder to the adapter Lampholder inclinable drive housing gt Unscrew a connector from the lampholder P Release the clamping screw gt Using the thread secure the lampholder to the drive housing P Tighten the clamping screw Lamp housing gt lampholder arm P Attach the lamp to the connector on the lampholder arm P Fit the filterholder complete with KG1 heat absorbing filter to one of the pegs e A tota
48. rinocular video phototube on the optics carrier in place of the binocular observation tube page 19 Partition of light e 50 to both eyepieces e 50 in the video photo beam path 35 Stop Before taking the photograph close off the right eyepiece to keep out stray light Move lever to the left Trinocular video phototube 100 Fitting See Trinocular video phototube 5090 Switchover gt Observation Push in lever gt Photography Pull out lever to the left Light partition setting VIS e 100 to both eyepieces Light partition setting PHOT e 100 to the left eyepiece e 100 to the video photo beam path Camera set up variants As shown in the set up diagram on page 34 there are various possibilities for adapting an MPS video digital or SLR camera Example With this equipment you can for example attach an MPS camera see detailed description in the MPS user manual an SLR camera or even a digital or video camera gt Fit the 1 0x video photo objective Example With this equipment you can for example attach a digital or video camera P Fit the video objective 2 on a Leica DC digital camera 3 with the C mount connection gt Connect the video objective with the mounted Leica DC digital camera on the right output of the video phototube gt Tighten the coupling ring 1 gt Fit the video phototube HU to the Leica stereomicroscope in accordan
49. sed on the Leica 55 MZ6 MZ75 and MZ95 the magnification is increased by the factor 1 25x This factor Is taken into account in the tables Magnification on Magnification on MZ125 MZAPO MS5 MZ6 MZ75 MZ95 1 0x Plan Planapo 1 25x Setting the working distance focusing obtaining a sharp picture e To focus use the focusing drive to raise or lower the stereomicroscope until the feature of interest is in focus The working distances of the various objectives are listed in the tables on pages 41 43 e The focusing drive can be operated from either the right or the left gt Place the object beneath the objective Engage the lowest magnification page 10 Look into the eyepieces Using the rotary knob bring the object into focus Focus approximately with the larger knob Focus accurately with the smaller knob Adjusting the free running of the focusing drive Does the focusing drive run too loosely or too tightly or does the equipment drift downwards You can match the ease of movement to the weight of the outfit and to your personal requirements Focusing drive P Grip the drive knobs firmly with both hands and turn them in opposite directions until you obtain the required degree of resistance during focusing Focusing drive coarse fine gt Turn the black ring on the right hand drive knob until you obtain the required degree of resistance during focusing 11 Use Microscope
50. ses in the fuse holder instead of the two 160mAT fuses see section For 100 120V power supply Changing fuses page 21 gt Connect the power cable to the socket of the base and to the power source Changing fuses Use Two 160mAT fuses for voltages between 200 240V Two 315mAT fuses for voltages between 100 120V see section Starting up page 20 gt Disconnect the power cable IS from the stand ne gt Pull out the fuse holder gt Fuse at rear Press out downwards gt Spare fuse at front Pull out sideways and insert into holder at rear P Push the fuse holder back into position again Transmitted or incident light e Connect an incident lamp to the transmitted light stand if required e Either transmitted light or incident light can be switched on gt For transmitted light Switch in position I P For incident light Switch in position gt Use thumb wheel to adjust brightness Switching on the transformer gt On the rear of the stand Use the thumb wheel to switch on the transformer and to adjust amp the brightness Operating the transmitted light stand Transmitted light bright field or inclined transmitted light gt Switch on transmitted light Transmitted light bright field e Bright field is suitable for examining translucent objects having contrasting structures The object is directly Illuminated from be
51. t of the socket in the stand e Transmitted light stand for bright and dark field Pull the fibre optic light guide out of the connector in the stand gt Remove the glass stage plate gt Carefully invert the transmitted light base and remove 7 Allen screws from the base P Lift off the lower part of the base gt Reverse the upper part of the base P Introduce 3 Allen screws into the cover plate from beneath and secure them to the bottom end of the side faced column gt Replace and secure the base gt Insert the glass stage plate gt Fit the microscope carrier and stereomicroscope as follows 18 Microscope carrier optics carrier binocular tube optical accessories In this section we will explain how to fit the microscope carrier and the optics carrier to the incident and transmitted light stands The combination of the optics carrier with the inclinable drive housing is described on page 22 The Leica motor focus system Is described in a separate user manual Side faced column drive housing The microscope carrier can be fitted in either of two positions to the drive housing refer also to page 12 P Use an Allen screw to secure the microscope carrier in the position required A connection socket for a 4mm dia earthing cable is provided on the microscope carrier Microscope carrier AX e The microscope carrier AX can be fitted to the drive housing only in positio
52. th us Not only before the sale but afterwards as well The business units of Leica Microsystems hold the management system certificates for the international standards ISO 9001 and ISO 14001 relating to quality management quality assurance and environmental management safety concept General directions Before you try to use the instrument please read the user manual and the safety notes Permitted uses The Leica 55 MZ6 MZ75 MZ95 MZ125 and MZAPO stereo microscopes are optical instruments which use magnification to see the finer details of objects close to the eye The objects may be either natural or created by human agency The basic outfit which includes a stand and a light source can be supplemented with various modules for photography TV dual station viewing and other applications Prohibited uses e The use of the instrument in a different manner from that described in this user manual can lead to injury malfunction and damage e Do not fit different plugs Do not dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manual Place of use e The Leica MS5 MZ6 75 MZ95 MZ125 and MZAPO stereomicroscopes are intended mainly for indoor use e fthe stereomicroscope is used outdoors protect it from dirt and damp Lamps and electrically operated stands from Leica may not be used outdoors Use in ESD protected rooms The optics carrier 55 MZ6 M
53. the object P Tighten the clamping screw gt Adjust the size of the light spot by moving the condenser lens forward or back P If necessary swing in a filter page 30 Changing the bulb Disconnect the lamp cable from the transformer Never touch the bulb when it is hot You may sustain burns e Do not touch the 6V 20W halogen bulb because fingermarks can cause it to crack when heated Remove fingermarks with a cloth dipped in alcohol gt Press the spring clips together and pull off the rear part of the housing gt Hold the new bulb in a cloth and push it into position P Press the spring clips together and close the housing again gt Centre the bulb Centring the bulb The 6V 20W bulb is a powerful source of illumination The more carefully you set it up the more uniform will be the lighting For this reason after fitting a new bulb recentre it gt Switch on the lamp owing out the diffusing filter Pull the condenser lens fully out vv v Hold the lamp and release the clamping screw v Point the lamp vertically down wards at a white sheet of paper so that two filament images are seen P Tighten the clamping screw P Using the slotted screw bring the two filament images into focus gt Displace the filament images so that they are opposite to one another but in contact gt Swing in the diffusing filter once more 31 Illuminators Coaxial illu
54. ttach a 6V 10W lamp to the step transformer P Set the voltage selector on the rear side to 115V or 230V P Attach the power cable to the power supply gt Connectthe lamp gt Adjust the brightness with the three step switch 4V 5V 6V Leica L2 Cold light source The antistatic Leica L2 cold light source is powerful small com pact inexpensive and Is suitable for simple observation tasks with the Leica MS5 and MZ6 stereo microscopes In addition to one or two arm light guides corresponding accessories are also available for the coaxial vertical and transmitted light illumina tion methods The Leica L2 can be directly connected on the stand The light guides can be mounted with arms in the threads on the microscope carrier For instructions on mounting on the clamp for fibre optic light guides see page 29 For a detailed information refer to the separate user manual Leica L2 M2 288 0de e When in use the Leica L2 fibre optic light source must I always be locked into an adapter to ensure stability Adapter gt 300mm focusing drive gt Secure the clamp to the 300mm focusing drive by tightening the screw QW gt Using the mounting rail slide the fibre optic light source into the adapter fork until it engages Adapter gt baseplate When mounted on the baseplate the light source can be used as a stand alone unit gt Using the mounting ra
55. vers see the same erect laterally correct three dimensional image The assembly and use of the discussion tube are described in the separate user manual M2 263 004 33 Accessories Video phototubes TV video photography digital imaging 1 2 3 1 2 1 3 SLR Leica 3 chip 2 2 3 chip 1 2 1 C mount CCD cameras camera housings MPS 30 MPS 60 B mount B mount C mount Leica DC cameras Photoautomats CCD camera camera CCD camera 11 543 703 11 543 702 11 543 706 a Adapter SLR 10 446 120 M prs 8x 7 I 10 445 304 10 162 226 10 10 445 305 16 10 446 261 B 10 445 541 11 541 006 1 1 0 150 10 445 929 i aa gt 10446 175 11 541 007 DC 500 10 445 928 0 5x 10 445 932 2 3 0 63x 11541016 232 1 2 0 5x 10 445 930 HU 10 446 174 1 0x 10 446 121 8x 10 446 131 10 10 446 132 16x 10 445 924 5090 10 446 229 100 ES Leica stereomicroscopes H HD A lt gt MS5 MZ6 MZ7s MZ9s 2125 MZAPO 10 445 931 10 446 197 10 445 925 10 445 927 34 Video phototubes e Video phototubes are designed to support modular photomicrography systems and Leica digital video film or SLR cameras e The video phototubes the modular photomicrographic systems and the digital cameras are detailed ina separate user manual along with instructions for their assembly and use e When you are not taki
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