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Bioquell ABS Class II Op Man (Revision 2)

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2. XX Denotes a preconfigured time which counts down in minutes The defaults are Evaporation 30 minutes Fumigation 240 minutes Purging 480 minutes These defaults can be changed by use of the Set up software Caution The operation of the automatic fumigating system does not require ventilation to an exhaust system however IT IS NECESSARY TO PROVIDE VENTILATION FOR THE ROOM It is recommended at least two air changes per hour this will ensure that all traces of formaldehyde are removed from the room 7 2 2 2 Carbon Filter Changing The carbon filter will complete a maximum of ten fumigating cycles as indicated on the cycle counter at the beginning and end of every cycle after which it ought to be replaced The filter is replaced by slackening the four screws Clamping the filter in place then lifting the top half up and sliding out the filter To fit the new filter follow the procedure in reverse Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 34 of 64 Web www bioquell com On fitting a new carbon filter the cycle counter will require resetting in the following manner a Press the key switch and enter the VAP code 2222 b Select 2 reset cycle counter to zero The removed carbon filter will be contaminated with formaldehyde Handling of the filter will require
3. Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 46 of 64 Web www bioquell com time entering values each time a cabinet is set up or a series of cabinets are to be set to operate in the same way However since controller sensor values are not stored in the configuration file it is essential that each cabinet is calibrated using the facility described in Section 11 3 Calibration Procedure 10 5 1 1 Load To load a configuration a Click on File followed by Load b All files with extensions cfg will be displayed select the appropriate by double clicking on it or type its name in the File Name box Click on OK or alternatively double click on the appropriate directory in the Directories or Drives boxes if it is stored c All the set up parameters will then be displayed on the screen 10 5 1 2 Save To save a configuration a Enter all the data in the relevant fields on the set up screen b Click on File followed by Save c Type a path and file name in the File Name box For easy management the choice of a file extension cfg is recommended this will store all files of this type together and will display them each time a Load Settings selection is to be made d Click on OK If a configuration file is to be used on more than one cabinet it should be saved on the
4. Cabinet Size Value above alarm point 1 0m 300 1 2m 400 1 5m 500 1 8m 400 The inlet velocity ought to be above the alarm point such that there are no nuisance alarms and an inflow of 0 45 m s 10 Ought to be measured The inlet velocity can be altered by using the slider if required c Dis Connect with the software and allow cabinet to stabilise at its operating set point This may take up to 15 minutes d Now check the cabinet inflow as described in section 11 3 2 e Validate the setup by performing the following test that simulates failure of the exhaust fan an airflow alarm must activate within 15 seconds i Allow the cabinet to run for at least 10 minutes in its final set condition with exhaust fan installed and PC disconnected ii Unplug the power lead to the exhaust fan and start a stopwatch iii Note the time to first activation of a flow alarm f The cabinet is now fully setup 12 2 SETPOINT ADJUSTMENT When the unit is running by default the downflow setpoint the point to which the downflow is controlled is half way between the high and low downflow alarms For most circumstances this is suitable however if the setpoint needs altering and the alarm points are to remain the slider labelled 8 in figure 16 is used To alter firstly Connect the computer and then alter the setpoint with the slider to the required downflow then Dis Connect The cabinet will now control at the
5. XX Denotes a preconfigured time which counts down in minutes The defaults are Evaporation 30 minutes Fumigation 240 minutes Purging 480 minutes These defaults can be changed by use of the Set up software 7 2 2 Automatic Fumigation Option K The Option K units have a special activated Carbon Filter designed to remove Formalin vapor The cabinet also automatically progresses through the fumigation cycle Each stage is displayed with time to run in minutes 7 2 2 1 Running an Automatic Cycle Add the formaldehyde solution to the deionised water This must be carried out in a fume cupboard or a Class I safety cabinet ducted outside Refer to the table in section 7 2 1 for quantities Close the front window and tighten both handles and lock Ensure that the blanking plate is fitted over the outlet of the top box The blanking plate is supplied with the cabinet and to fit remove the grey plastic spigot by unscrewing the 6 screws then replace with the white blanking plate Ensure that the white rubber gasket is fitted between the plate and the top box It is essential that the plate is fitted to the cabinet to eliminate any possibility of leakage into the room see section 14 Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 32 of 64 Web www bioquell com Figure 14 showing the blankin
6. Clarus Fumigation The cabinet ought to be checked once the Clarus is in gassing with a portable H202 sensor or equivalent to check that there is no leakage Check specifically around the seals and the exhaust spigot if not ducted After 16 minutes the fan on the cabinet will automatically pulse to ensure there is good gas distribution It will pulse again after a further 14 minutes During the fan pulsing the cabinet will display Clarus cycle Circulation d The next message seen on the display will be Clarus Purging The Clarus will have just started or is about to start Aeration When Aeration does start it is highly desirable to exhaust the peroxide Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 27 of 64 Web www bioquell com Revision 2 Fax 44 0 1264 835801 through the duct work or temporary vent to atmosphere as the time taken to purge will be dramatically reduced CHECK WITH THE HEALTH AND SAFETY OFFICER FIRST THERE WILL BE HIGH LEVELS OF HYDROGEN PEROXIDE IMMIDIATELY LOCAL TO THE EXHAUST OUTLET To purge up the ductwork or temporary vent Press the Fan key 1 and enter the correct PIN and open the front screen window slowly this will cause the cycle to transition to Cabinet purge mode with air being taken out the exhaust The following will then be displayed Purging XXX mins to run WA
7. 17 05 2006 correct inflow velocity reading is achieved 3 months 6 months 9 months 12 months Disable _ No change Continue Main Fan Speed s o 3491 E Exhaust Speed ES SE Figure 21 Low Inflow Alarm k Set the Exhaust Speed slider below the Main Fan Speed slider to the value indicated below Class 2 ABS Mk2 Fan Setting 1 0m 2850 1 2m 3000 1 5m 3200 1 8m 3500 I Following a similar process to that described in paragraphs g and h This time either the anemometer will need to be positioned in the exhaust airstream or use reading from an installed devise or inflow measurement at the average position Set the inflow velocity to 0 4 m s Section 11 3 2 Inflow Velocity gives the methods of measuring and calculating the inflow air velocity To reduce the inflow velocity it may be necessary to block off duct work partially shut dampers or alter inverters where the cabinet is not controlling the exhaust fan Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Hampshire SP10 5AA United Kingdom Page 55 of 64 Web www bioquell com cl Fax 44 0 1264 835801 Revision 2 Email enquiries bioquell com m After the three alarm points have been set a dialogue box will offer the choice either to Continue which will store the data to the controller s memory or to Cancel the calibration process If the latter is selected then flow settings and alarm values in the cont
8. Check that the alarms are set as below in box 6 in figure 16 High Downflow alarm 6a shall be set to 0 41m s 0 02m s Low Downflow alarm 6b shall be set to 0 25 m s 0 02m s Low Inflow alarm 6c shall be set to 0 4 m s 0 02m s A dialogue box will now appear similar to the illustration of Fig 20 With the anemometer at a position representing the average velocity adjust the fan to give the High and low downflow alarm values Adjustment is made by dragging the slider next to Main Fan Speed title on the screen Alternatively for small increments the bar either side of the slider may be clicked or for the smallest adjustment either direction arrow may be clicked Bioquell Walworth Road Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 53 of 64 Web www bioquell com STD9000 002 Tele 44 0 1264 835800 Revision 2 Fax 44 0 1264 835801 89 Setup Program Fle EIN ros Con MICROFLOW Contaminz pen Figure e Opening Dialogue Box E Class II Bsc Setup Program V2 44 File Pi noe CommPort Options Messages Languages Serial No MICROFLOW Calibrate Flow Control Contamination Control Position anemometer in an appropriate position 17 05 2005 11 16 inside the cabinet to set the low downflow E 17 05 2005 GER alarm d Adjust the fan speed slider until a velocity J 17 05 2006 of 0 25 mis is shown on the anemometer C 3
9. iii If the cabinets airflow pattern has been recently disturbed e g exhaust obstructed In any of these cases the alarm can be expected to clear and after waiting a few extra seconds If an airflow alarm is still displayed after 3 minutes from switching on the cabinet the unit should not be used and advice sought from your Safety Officer or Supplier Bioquell Walworth Road Hampshire SP10 5AA United Kingdom STD9000 002 Tele 44 0 1264 835800 Revision 2 Fax 44 0 1264 835801 Email enquiries bioquell com Page 13 of 64 Web www bioquell com 5 OPERATION 5 1 CONTROL PANEL The touch controls are located on the Control Panel Fig 3 and are described in the following paragraphs READ MANUAL em e o t 1 SELECT FAN BUTTON Kei ko 2 OPEN WINDOW ar MES See d e 2 2 d s 4 IF ALARM SOUNDS NOTIFY SAFETY OFFICER 293 BE we EAR 6 1 dx D vA D ew Figure 3 Control Panel for illustration purposes only 5 1 1 Mains Power Indicator e The blue indicator is lit when the mains supply has been connected to the cabinet The indicator remains lit at all times 5 1 2 Alarm indicator The alarm indicator flashes whenever an alarm condition occurs j d Additionally unless muted by pressing the MUTE button the buzzer emits an alternating two tone signal to give warning of an alarm condition 5 1 3 Fan Control Operation of this switch causes the cabinets in
10. rennen mene 2 1 Responsibility of the User eeesseeessieeesessseeeeeeee nnne nennen ener nnns trn nennen 3 SPECIFICATION m E HH 3 9 3 10 Operating Environment ener trne en nent ennt sn nenn nnns CONST CHOI m M Diriensions Welglit icti redit nene bus tee eae bt d eere rebut id mg mE VT alle BA Em Non Ret rn Valves retten eet HR Run enge Pugna qne be expand ne naai ieu Res Bronce aif E E re pisce Ae INSTALLA TONG mE 4 1 4 2 4 3 POSITIONOL the cabine RC ETTREU Position of the Fan for Ducted Cabineis nnns Preparation FOr USC er 5 OPERATION RE 5 1 CO1O101010101010101C1 c1 zik gt OOMONDaOA WD Eu tre Re TEE Mains Power Indicator eene nennen nnne nennen nnne nnns Alarm teller Fan Contro EM IETLLEMPO Fam Off Delay seis E ENE gu NET eine e Gas SUDDIY e A A E E A E E 5 1 12 Keylock and PIN Number Change 5 2 Sliding window And Fan Operation nennen enne nennen 5 2 1 Integral Bypass Operation 5 3 5 4 5 5 Work Surfaca MUTET PEE Cabinet Mains Power Connection esee ennenen enhn nennen enses snnt n nenne en
11. 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 49 of 64 Web www bioquell com 11 CALIBRATION OF AIRFLOW AND ALARMS 11 1 INTRODUCTION The setup software Calibrate facility allows calibration of the cabinet airflows and alarms This option will allow downflow and inflow alarms to be set together with the operating speed for the cabinet It is then this speed that the control system will use as a reference and always attempt to maintain The cabinet airflow can be set up to comply with EN12469 2000 or another criteria and the requirements procedures are described in the following paragraphs Note that re circulatory cabinets are set up accurately with a calibrated rig giving accurate inflow and downflow readings Site calibrations will always be less accurate so it is important to double check before altering any settings The Calibration and set up procedure only covers cabinets with an exhaust fan either in the top box or at the end of the duct For single fan units Option A the calibration procedure is different and requires different software refer to BIOQUELL for details 11 2 EQUIPMENT REQUIRED e A hot wire anemometer for downflow measurements a 100mm Dia Paddle wheel anemometer can be used instead e A Laboratory stand to hold the anemometer e f Re circulatory cabinet or no existing installed duct airflow measuring devise a 10
12. 5 1 12 Keylock and PIN Number Change Keylock 7 This button provides the facility to lock the buttons associated with On fan control Then when ever the fan control is to be altered e g turn it OFF or ON the PIN must be entered This is a requirement under EN12469 but the units are shipped without this facility activated see below PIN Number Activation and Change Pressing the button followed by entering the appropriate PIN default 1111 will prompt the user to either Change the PIN or Lock the Cabinet If Lock the cabinet is chosen then it is necessary to press the keylock button and enter the PIN and unlock the cabinet before the fan can be operated 5 2 SLIDING WINDOW AND FAN OPERATION The cabinet has a sliding screen which produces a complete seal when shut This negates the use of a night door when de contaminating To open the Front Sliding Window from the closed position The cabinet will be displaying the following message WARNING Screen locked shut Release the door frame by turning the two black T handles on either side of the window a half of a turn anti clockwise The frame will Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 16 of 64 Web www bioquell com move 20 30 mm outwards It is important the both T handles are turned so that the red dot is at t
13. reconnection of the power will cause the fan to start However all other functions will be automatically set to off and if required they must be reselected If Fumigating with Formalin the cycle will re start automatically from the beginning of the phase it was in when the power disconnection occurred 5 5 3 Hour Meter and Service Date Due These parameters can be viewed on the display by pressing the TEST button Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 20 of 64 Web www bioquell com 6 OPTIONAL EXTRAS Figure 9 Services Taps and Mains Sockets 6 1 SOLENOID OPERATED GAS INLET Gases for use inside the cabinet are supplied via a solenoid valve located behind the side panel which permits gas to flow only when the cabinet fans are operating and airflow levels are within the specified parameters Switch on the gas supply by pressing the button marked GAS on the Control Panel The gas flow can then be controlled at the tap 6 2 MAINS SOCKET A single or double mains socket can be fitted to the side wall of the cabinet Most cabinets are fitted with two similar electrical sockets Equipment in excess of 300 W should not be connected to a socket if this value is exceeded the circuit breaker will trip The circuit breaker is located on the top right of the cabinet adjacent to
14. 10437 E Fuses 1 100 mA Antisurge EHB 10174 E Fuses 1 1 A Antisurge EHB 10440 E Door Seal 6m Seal 550 0009 Circuit Breakers 1 Double Pole Circuit Breaker Switch 2 5 A 250 0010 ABS PCB 1 Main circuit board 259 0994 ABS Top Box Duct Spigot 1 Duct Spigot 165 2000 Flex Ducting Flex Ducting 150 0300 2 on 1 8m version SPARES LIST AUTOMATIC FUMIGATION SYSTEM ITEM QTY USED DESCRIPTION PART No Filter 1 Carbon ABS 013 Fan 1 230 V 50 Hz 450 2053 Valve Flap 2 Rubber 550 9000 Valve Solenoid 2 230 V 50 Hz 221 1060 Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Email Hampshire SP10 5AA United Kingdom Page 58 of 64 enquiries bioquell com www bioquell com Web 14 SERVICING RECORD DATE ACTION COMPLETED BY Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 59 of 64 Web www bioquell com 15 EC DECLARATION OF CONFORMITY EC DECLARATION OF CONFORMITY FOR ATTACHMENT OF CE MARK Ce We the manufacturer BIOQUELL UK Limited Walworth Road Andover England SP10 5AA Tel 44 0 1264 835835 Fax 44 0 1264 835836 declare under our sole responsibility that the appliance ABS CLASS II Microbiological Safety Cabinet Serial Number as stat
15. 7 4a 4b 4c 4 6a 6b 6c 6 Read Clear All Dis Connect Calibrate Write Figure 16 Typical Controller Set up Screen 10 4 CUSTOMISATION OF CONTROLS A number of parameters relating to the use of the cabinet can be customised according to individual preference or requirements these consist essentially of timer settings updating of the controller clock and language of the messages and prompts shown on the display The numbers of the following descriptions relate to the features in Fig 16 Note Text in bold found in the following descriptions relates to options found in the set up screen Fig 16 clicking the left mouse button with the pointing device on the symbol its adjacent box or either of its buttons whichever is appropriate will effect a routine or select the appropriate option 1 Controller Clock The controller clock is automatically updated whenever communication is established between the computer and controller Ensure that the computer date time is correct adjusting it if necessary using the date time function To update the clock click on Connect If all other operations are completed break the computer controller link by clicking on Dis Connect Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 43 of 64 Web www bioquell com 3 Timer Settings The settings for the timers
16. MS e The cabinet will prompt the user to open the window after a few seconds with a message Open Window and the sounder will operate e Internal Fan starts Cabinet will stabilise this takes one minute The cabinet will then display SAFE MODE NOTE If the User opens the window then the cabinet will Alarm and the following message will appear Window Open Fan not On To switch off the Cabinet and extract air from the room Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 18 of 64 Web www bioquell com EITHER e The window is shut e Internal fan stops e Damper will close off cabinet OR 3 3 Fan button can be pressed Internal Fan stops Cabinet will alarm prompting the user to shut the window Damper will close off cabinet If the fan requires switching off and the window is required to remain open then this can be achieved without the Cabinet alarming with the following procedure ef e Fan Time delay button is pressed e Cabinet continues to run for a pre set time e The damper will close off the cabinet WORK SURFACE The actual working surface is lower than its edges so that minor spillages will be contained The worktray can be removed for cleaning purposes To remove the work tray first lift up and out a side infill panel using the finger notch When at the back this
17. MS Windows installed and a serial port The PC s monitor should support a screen resolution of at least the VGA standard Physical connection of the computer to the cabinet is now required by means of a suitable cable Serial Cable 9 way male D Connector to 9 way female D Connector Serial Cable 9 way male D Connector to 25 way female D Connector USB RS232 Converter Cable 9 way male D Connector to USB Connector Connection to the controller Cabinet is via the 9 way female D connector located under the front cover on the side of the electrical box The computer end of the cable should be connected to an available Comm port See figure 15 The connector on the computer will thus be either a 9 way male D Connector 25 way male D connector USB Port Note Important The comm port used to connect to the cabinet must be configured as either COM 1 or COM2 Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 41 of 64 Web www bioquell com Electrical Box omm Port 1 or 2 Communication 259 0990 Cable Eprom May RS232 Port a b or c above Differ with Amplifier 259 0994 256 5435 1 Lap Top Computer Class 2 Controller ABS 4 Way Cable Running PCB Assembly Assembly V2 40 Software For Re circulation Cabinets Non Fan Assisted Exhaust or
18. SEXES at any time If UV lamp is in use or the UV lamp switch has been pressed UV lamp switch LED lit it will automatically be switched off when the fluorescent light is selected 5 1 9 UV Lamp X WARNING d DUE TO ITS HAZARDOUS EFFECTS THE CABINET OPERATOR d SHOULD AVOID ANY EXPOSURE TO UV RADIATION Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 15 of 64 Web www bioquell com When selected the UV lamp will remain on for a configurable time period after which it will automatically switch off The default setting is 15 minutes The time period for which the UV lamp remains on can be changed see Section 10 Control System Customisation and Calibration 5 1 10 Vaporisation Cycle Pressing this button initiates the decontamination process See Ua Section 7 Decontamination of the Cabinet for the procedure 8 5 1 11 Gas Supply As a Safety feature in series with the gas supply tap is a solenoid valve which is controlled from the control panel Thus the gas 9 supply can only be selected if the airflow is in a safe condition i e no alarms are active Once the valve has opened gas supply can be controlled via the tap inside the cabinet in the usual way Caution The solenoid valve will automatically close the gas supply if an alarm occurs during the time it is selected
19. V2 44 Software For Ducted or Fan Assisted Exhaust Cabinets Figure 15 Communication Cable 10 3 INSTALLING THE SET UP PROGRAM To install the BSC Set up program on the computer use Explore and install the program by selecting the file named Setup in the same way that other programs are installed To check if the operating system installed on the computer is compatible with the program refer to BIOQUELL After completing the installation an icon will be created Double click on the icon a computer terminal labelled BSC to run the Set up programme The screen shown in Fig 16 will be displayed The screen layout has been designed for use with a variety of screen devices for this reason it should always be used in full screen mode or it is possible only part of the set up blocks will be visible When starting the SET UP programme should the error message COM 1 or COM 2 Unavailable appear Click comm port See section 10 5 3 on the top menu bar and select the opposite to message displayed in the drop down menu Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 42 of 64 Web www bioquell com 10 pos 07 02 00 15 53 1 T J j Disabled 3 months O 6 months au 7 9 months 12 months TI Disable No change
20. Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 24 of 64 Web www bioquell com 7 DECONTAMINATION OF THE CABINET It is recommended that the cabinet be kept clean and free of dust by swabbing down or washing down with a suitable disinfectant Disposable gloves should be worn for this task Do not use any Chlorine based cleaning agents The cabinet should be kept clean and free of unnecessary equipment Decomtamination would be necessary at the following times a Before carrying out any maintenance work b Before changing high efficiency filters c Before performance testing is carried out d Before moving relocating or in any way disturbing the cabinet e Before instituting a change in the work program f After a gross spillage as defined by your Safety Officer g To decontaminate any equipment or materials before their removal from the cabinet h Periodically as required by your Safety Officer 7 DECONTAMINATION WITH HYDROGEN PEROXIDE This method of bio decontamination requires a BIOQUELL Clarus Hydrogen Peroxide Gas Generator alternatively the Bioquell EBDS Hydrogen Peroxide Bio decontamination service The following instructions relate to the use of a Peroxide Cabinet and a Clarus L A Clarus C can be used refer to Bioquell for details If the unit is not a Peroxide Cabinet the Bioquell EBDS service can still bio dec
21. allows access for the whole tray to be removed Finger Notch Figure 7 replacing the side infill panel ensure that the slots running down it are facing into the cabinet Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road ici Fax 44 0 1264 835801 Hampshire SP10 5AA Revision 2 United Kingdom Page 19 of 64 Web www bioquell com Email enquiries bioquell com 5 4 CABINET MAINS POWER CONNECTION Mains power connection to the cabinet is made by the use of a standard IEC 3 pin connector assembly incorporating dual pole fusing and an isolator switch Fig 8 Figure 8 Mains Connector and Switch 5 5 SAFETY CONTROL FEATURES 5 5 1 Automatic Air Velocity Control The control system automatically compensates the airflow for the dirtying of filters and variations in the mains power supply Whenever the airflow is within safe operating conditions the display reports the message CABINET SAFE If an alarm condition is detected the buzzer will sound and the alarm LED will flash the cause of the alarm will be reported on the display After switching on the cabinet from cold an airflow alarm may occur fora short period This is due to the stabilisation of flow sensors and airflows During stabilisation the display shows the message STABILISING FLOW 5 5 2 Automatic Fan Start After Power Failure In the event of power disconnection from the cabinet while the fan is operating
22. at a relative humidity of greater than 60 for it to give the designed operator protection Installation category overvoltage category II BS 7671 Microflow is a registered trademark of BIOQUELL plc Windows is a trademark of Microsoft Corporation Bioquell TM003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 5 of 64 Web www bioquell com 2 INTRODUCTION AND PRINCIPLE OF OPERATION The Microflow Cabinet is the result of many years experience in the development and use of Safety Cabinets and should give long and trouble free service provided these simple instructions are followed The Microflow ABS Class II Cabinet Mk II has been designed to EN 12469 2000 which provides operator product and cross contamination protection This is achieved by means of a uni directional downward airflow inside the cabinet and an air curtain at the front aperture The Class II cabinet is designed for working with Category one and two pathogens The critical airflow requirements of the cabinet to ensure that it functions correctly are the inflow and downflow The mean inflow must be greater than or equal to 0 4 m s This parameter is monitored by an airflow sensor on the exhaust If the airflow should fall below the 0 4 m s limit then the cabinet will alarm The mean downflow velocity must be between 0 25 and 0 5 m s If th
23. new setpoint Bioquell TMO003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 57 of 64 Web www bioquell com 13 SPARES Refer to the Supplier or the Maintenance Provider for a comprehensive list of spares Below are the most common parts requested MODEL ABS 1000 ABS 1200 ABS 1500 ABS 1800 Main Filter TM021 0007 TM022 0007 TM025 0007 TM028 0007 Exhaust Filter amp TM029 0009 TM029 0010 TMO029 001 1 TM029 0012 Integral Bypass Filter Exhaust Filter Option TM029 0030 TM029 0031 TM029 0032 TM029 0033 A only Light Tubes H12010018 H12010015 H12010019 H12010037 UV Tubes 257 0512 257 0511 257 0511 257 0511 Flexible Plenum TM021 0010 TM000 0033 TM000 0033 TM021 0010 RHS amp LHS Gas Springs 754 1023 754 1026 754 1022 754 1021 ITEM QTY USED DESCRIPTION PART No Main Fan 230V 1 Motor Fan Unit complete with Capacitors 450 2050 Top Box Fan 230V 1 Motor Fan unit in top box on 1 0 1 2 amp 1 5m 450 2052 Top Box Fan 230V 1 Motor Fan unit in top box on 1 8m 450 0110 Starters for UV 1 Starter BHE25013E Electronic Ballast 1 Ballast and starter for Fluorescent tubes H12010020 Fuses 2 10 A Antisurge EHB 10176 E Fuses 3 6 3 A Quick Blow EHB 10342 E Fuses 1 2 A Antisurge EHB 10374 E Fuses 2 1 A Quick Blow EHB
24. of cycles run with a particular filter The fumigation cycle should be stopped by using the VAP switch and entering the PIN and then the blanking plate ought to be removed and replaced with the spigot Only then the window ought to be unlocked and the cabinet set to normal operation Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 33 of 64 Web www bioquell com To abort the cycle at any point press the VAP key then enter the PIN and press the Fan button to start the cabinet A description of the cycle is provided in Table 2 Table 2 Automatic Fumigation Cycle ACTION PROCESS DISPLAY MESSAGE Lock shut window Fit blanking plate and pour formalin into vaporiser Press VAP Start Cycle Enter PIN Number Enter four digit Number Vaporiser powered to Evaporation xx mins to run evaporate formalin Vaporiser powered for xx minutes Formalin sterilises inside Fumigating xx mins to run of the cabinet Fan circulates fumigant for Circulating one 5 second phases Fumigation completed Filter fan will start to Purging xx mins to run purge fumigant through activated carbon filter Purge time expired Cycle complete Press VAP amp re enter Cabinet returns to normal WARNING Screen locked password operation shut Remove blanking plate and open window
25. the mains connection socket and on off switch see figure 8 Electrical safety test on the electrical socket has a maximum permitted earth leakage of 0 5 mA 6 3 SERVICES TAP In addition to the solenoid operated inlet a second tap without an interlock valve is provided This valve can be used to connect air vacuum or non hazardous gas supplies to the cabinet 6 4 HYDROGEN PEROXIDE CONNECTIONS On Peroxide versions of the cabinet connections designed to enable integration with a Clarus Hydrogen Peroxide Generator are fitted This is used to de contaminate the cabinet see section 7 6 5 FORMALIN VAPORISER A Formalin Vaporiser Fig 10 can be fitted to the cabinet to provide a convenient method for decontamination The vaporiser can function as part of the integrated fumigation cycle Refer to Section 7 Bioquell TM003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 21 of 64 Web www bioquell com V a SS A Figure 10 Formalin Vaporiser 6 6 FIREBOY GAS BURNER This burner has been designed for use in Safety Cabinets and Laminar Flow Units and can be supplied with a foot operated switch The switch operates a solenoid valve allowing the gas to flow and at the same time initiates its ignition The burner can either be set for a fixed time of operation or can be extinguished by pressing the foot switch
26. 0mm Dia paddle wheel to measure inflow velocity e Multimeter e Laptop Computer with V2 44 Calibration software Installed e Communication Cable see section 10 1 11 3 AIRFLOW REQUIREMENTS Mean downflow velocity shall be between 0 25 and 0 5 m s as stated in EN12469 No individual measurement of eight shall differ from the mean by more than 20 Note set the upper alarm to 0 41 to automatically get a downflow set point of 0 35 m s 10 The inward airflow velocity through the working aperture shall not be less than 0 4 m s An average of approximately 0 45 10 is required 11 3 1 Downflow Velocity Airflow velocity measurements shall be made in a horizontal plane 100 mm above the top edge of the working aperture Measurements should be made for at least 1 minute in each of eight positions four along a line of the Bioquell TMO003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 50 of 64 Web www bioquell com depth of the working space forward of the rear wall and the remainder along a line the same distance behind the front window Along each line measurements should be spaced at and of the width of the working space measured from each end I D L I H 4 L L I I I L L H Side vent Front Grille R H Side Vent Figure 17 Downflow measurement positions It is recommended t
27. 1 Fumigation with an Integral or Separate Fumigation Unt 30 7 2 2 Automatic Fumigation Option ki 32 7 2 2 1 Running an Automatic Cycle sse ener 32 7 2 2 2 Carbon Filter Changing esses enemies 34 9 Gassing the Laboratory 4 etes cideces Lese cer dates tente det eant e ocean ee ean rea eo dea Bee ddr pad 35 8 agde 36 8 1 Daily e E E Em 36 82 E e EE 36 8 3 Annual Maintenance Testing and Re Calibrations ssssnssssnnnseernesesn nnen nrnesetnnnne nn nnne nnne 36 8 4 cyst P E 37 8 4 1 dI Ult 37 8 4 2 Be ue d 38 8 5 Fluorescent Tube Replacement sse menn nene n nennen nnne 38 8 6 UV Tube Replacement eee Irsa eter ea Eee edet reru 38 SOMME el guni ei m EE 38 MEC Ais wipe lem 39 9 1 Bio Organism Removal Cleaning ener entrent 39 9 2 Stainless Steel Components nennen nnn entente sn nnns sinet 39 9 3 GEELEN 39 9 4 Painted and Other Components ennt enters nnn intrent 40 10 CONTROL SYSTEM CUSTOMISATION AND CALUIDRATION eene 41 10 1 nisse UT 41 10 2 Computer and Cable requirements eene 41 10 3 Installing The Set Up Program 42 10 4 Customisation Of ele EE 43 10 5 MITES 46 TOS Fil TT 46 LU S Long Em 47 UG E 47 UEM S0 mEP 47 uM PIN NOS 47 USE o 48 UE Lc 48 10 5 3 COMM POR E 48 10 54 ESI CC
28. BIOQUELL pee lt s DIS ADVANCED BIO SAFETY CABINET ABS CLASS II USER MANUAL Bioquell Walworth Road Hampshire SP10 5AA United Kingdom STD9000 002 Tele 44 0 1264 835800 Revision 2 Fax 44 0 1264 835801 Email enquiries bioquell com Page 1 of 64 Web www bioquell com REV DESCHPTON DATE C R NO ORIG CHKD APPRD 2 Minor corrections clarifications to sections 10 11 amp 12 8 Septos PF ORC TI LLL LL LL S EE r4 ES EE DEE E EES EE EE Caution Please read this Manual thoroughly before attempting to use this unit If you do not understand or are unsure of any information contained in this Manual please contact your Agent or BIOQUELL for clarification BIOQUELL designs manufactures and supplies a broad range of containment and airflow products as well as bio decontamination solutions for Rooms Systems and processes Laboratory equipment Biomedical equipment For further information and contact details refer to our web site www bioquell com Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 2 of 64 Web www bioquell com CONTENTS NERIS IM 1 1 Safety EnvlrOnmental Conditions AA 2 INTRODUCTION AND PRINCIPLE OF OPERATION
29. ET CABINET Height mm 1580 1580 1580 1580 including top box Depth mm 748 748 748 748 Width mm Internal 1000 1200 1500 1800 External 1190 1390 1690 1990 Weight kg Without top box 185 210 230 290 With top box 200 230 270 310 The maximum load of 25kg which can be placed on the cabinet floor with a foot print of 300mm by 300mm Large point loads must be avoided 3 4 ELECTRICAL Always disconnect the mains supply before removing any cover displaying the following label WARNING Disconnect the mains supply before removing this cover The ABS cabinet requires a 230 V 10 50 Hz single phase supply Power W 1 0 m 1 2m 1 5m 1 8m CABINET CABINET CABINET CABINET Without Top Box 700 800 800 1150 With Top Box 950 1150 1150 1500 The internal control system operates at 5 V Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 7 of 64 Web www bioquell com 3 5 WORKING AREA The working are is made of high a grade stainless steel tray The tray is dished to contain any spillage The table below gives the working area for the different cabinet sizes Height Width Depth mm mm mm 1 0m Cabinet 570 865 420 1 2m Cabinet 570 1065 420 1 5m Cabinet 570 1365 420 1 8m Cabinet 570 1665 420 The safe working area is shown
30. RNING THERE WILL BE HIGH LEVELS OF HYDROGEN PEROXIDE IN THE CABINET CARE MUST BE TAKEN WHILE THE FAN IS STARTING AS SOME PEROXIDE MAY EXIT THE WORKSPACE DO NOT FULLY OPEN THE WINDOW OR INHALE AIR FROM THE WORKSPACE UNTIL SAFE LEVELS OF PEROXIDE HAVE BEEN ESTABLISHED At the end of Purge mode the cabinet will go back to normal operation and this will coincide with the Clarus L completing its Aeration Check the level of Hydrogen Peroxide in the cabinet by independent means if below 1 ppm the cabinet is safe to use No external Purge If it is not possible to purge externally the Clarus L can carry out the Aeration then the cabinet ought to be left with the message Clarus Purging When it has been deemed safe through independent sampling of the cabinet s air the Aeration phase on the Clarus L can be completed and the cabinet returned to normal running by selection of the Vapor key 7 and entry of the correct PIN Check the level of Hydrogen Peroxide in the cabinet by independent means if below lppm the cabinet is safe to use Running a Workspace Decontamination Cycle a Runa decontamination cycle with the Clarus according to the Clarus manual The following cycle parameters ought to be set for bio decontamination of the workspace Conditioning Time 10 minutes Dwell Time 10 minutes Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell c
31. a Safety Control Fealtlfes i cedit ce Ente rt xad eb contu pere ra eec cae Egan d 5 5 1 Automatic Air Velocity Control 5 5 2 Automatic Fan Start After Power Failure sess 5 5 8 Hour Meter and Service Date Due nennen nene nn nnn nnne nnne nnne nn 6 OPTIONAL EXT RAS eerta initi ite cu acce ctt od d d iu geed 6 11 6 11 6 11 6 11 Solenoid Operated Gas Inlet eesssssssssssssssssseee entrent ennt entere MaliNS SOCK o SIM itE Hydrogen Peroxide Connections esses entente nennt snnt nnne Formalin 1e m Bizi ACTAS To ch o matic Gas BUMMER cisci eie sagedennad erani c totci sedan oae EU ERAN eB CAE qeu RR Le ef vcn M Volt green Internal Socket Residual Current Device sss HA En noH A High Level UV Ligii Em 2 Portable UV Be TE 3 Remote Start Stop Switch sss eee enne 7 DECONTAMINATION OF THE CABINET seen nre nennen nnns 7 1 Decontamination with Hydrogen Peroxide essen nennen Bioquell Walworth Road Hampshire SP10 5AA Email enquiries bioquell com United Kin STD9000 002 Tele 44 0 1264 835800 Revision 2 Fax 44 0 1264 835801 gdom Page 3 of 64 Web www bioquell com 7 2 Decontamination with Formalin cessere nn nennen 30 7 2
32. a Position of Paddle wheel when measuring inflow velocity Wi a L dNTAKE GRILLE Wi The following table gives the position of the paddle wheel across the window Cabinet No of test Distance between test points in mm Size points 1 0m 4 125 250 250 250 125 1 2m 4 150 300 300 300 150 1 5m 4 187 375 375 375 187 1 8m 4 225 450 450 450 225 The average from the readings ought to be calculated and then this will give the average inflow 11 4 CALIBRATION PROCEDURE Calibration ought only to be carried out by a suitably trained Technician The calibration process will instruct the operator to set the airflow via a virtual slider on the screen to the three alarm velocities corresponding to low downflow high downflow and low exhaust representing low inflow These values will then be used by the setup software to derive an operating condition for the cabinet such that no alarms will be activated When the data is written to the controller the values will be stored and used until the cabinet is next calibrated or adjusted The operating condition of the cabinet can be manually adjusted if desired The cabinet ought to be checked first to see that it does need re calibration as the cabinet will have been calibrated at the factory and checked at installation and adjusted if required Bioquell STD9000 002 Tele 44 0 1264 835800 W
33. a second time The advantage of this type of burner is that the flame is only present when actually needed and so causes minimum disturbance to the airflow pattern within the cabinet 6 7 TOUCH O MATIC GAS BURNER This is a gas burner which is operated by a palm control The control is linked to the gas burner and operates a valve to allow passage of gas a pilot flame in the burner ensures immediate combustion of the gas The flame is extinguished by the release of the palm control and its size and intensity can also be varied 6 8 STAND For applications where bench space is not available for the cabinet a stand is available with either adjustable feet or swivel castors The height of the stand can be adjusted between 675 and 850mm in 25mm increments at installation to suit the operator This gives a work surface height of 745 to 920mm In addition there is a foot rest at the front which can be moved to also suit the operator A stand with motorised height adjustment is also available This allows the stand s height to be altered to the operator s requirement Using the control pendent three heights can be programmed in along with a maximum height to prevent the cabinet from being raised too high 6 9 VOLT FREE CONTACT An option is available so that continuous remote monitoring of cabinet operating status can be performed Interfacing to the cabinet is via a multi way plug located on the top surface of the cabinet Bio
34. abinet 180 334 375 459 1 5m Cabinet 200 420 473 578 1 8m Cabinet 200 507 570 696 The remote fan must be able to be set at the airflows given Bioquell can supply a fan designed to be controlled from the cabinet The cabinet can control a third party fan which is powered by a single phase 230V motor of maximum power of 400W and is suitable for Phase Angle control Alternatively an inverter drive system can be used as long as it can accept a 4 20mA speed control signal for this an additional board is required to be fitted in the cabinet We recommend the use of 160 mm nominal diameter rigid PVC ductwork except where the duct work is excessively long or has multiple bends The connecting spigots on both the Cabinet and Fan Box have been manufactured to match this diameter of ductwork so the connections may be made using soft PVC sleeves that slide over the ducting and the spigot and are retained by stainless steel bands NOTE to change the filters the top box plenum has to be raised vertically by a minimum of 20mm as a result the duct work must have a flexible connection to cater for this If the duct work is 2m or less the internal fan on the cabinet can be used If longer ductwork runs are required refer to Bioquell for advice 4 3 PREPARATION FOR USE In order to utilise the full operator and product protection factors given by the cabinet it is essential that the operator is fully familiar with the cabinet and all of i
35. alarm indicator will flash and unless muted by pressing the Mute button the buzzer will emit a two tone sound 3 8 LIGHTING Fluorescent tubes provide a lighting level in excess of 750 Lux over the working area 3 9 FILTRATION High efficiency filtration is provided by narrow pleated HEPA filters meeting the requirements of class H14 or higher of EN 1822 1 Bioquell Walworth Road Hampshire SP10 5AA STD9000 002 Tele Revision 2 44 0 1264 835800 Fax 44 0 1264 835801 Email enquiries bioquell com United Kingdom Page 9 of 64 Web www bioquell com 3 10 CONFIGURATIONS Description Top Box Integral Double Flap 2M Rigid Remote Low Plenum Exhaust Exhaust Valve Ducting Exhaust Level Fan HEPA Fan version Filter Required Option A Option C X X X Option D X X X Option F X X X Option K X X Integral Bypass X X X Option A Option C Option D Option F Option K Re circulatory Cabinet with single exhaust Standard ducted cabinet single HEPA exhaust exhaust fan 2m or less duct work to outside of building using internal Re circulatory Cabinet with double HEPA exhaust and internal exhaust fan As Option F but with automatic Fumigation facility Integral Bypass Cabinet as Option C but with room air intake when cabinet not operating the room air can be HEPA filtered Bioquell Walworth Road H
36. alworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 52 of 64 Web www bioquell com To calibrate the cabinet if this is required proceed as follows a b c d e f g Establish a connection between the PC and controller as described in Section 10 5 1 Dis Connect The cabinet ought to be running for at least 10 minutes with the window secured in the working position Set the Main fan slider labelled 9 on figure 16 to the values given below Class 2 ABS Mk2 Fan Setting 1 0m 2500 1 2m 2750 1 5m 3050 1 8m 2400 Set the Exhaust Fan slider 9b on figure 16 to zero which will turn the fan off Measure the downflow as described in section 11 3 1 Once the average downflow reading has been calculated the position of the single downflow reading closest to this average should be used to set the downflow alarm points Initiate the software calibration process by clicking with the pointer on the Calibrate icon video screen with sine wave see box 11 Fig 16 The configuration file opening screen will then appear see figure 19 If a cabinet of the same type has been previously calibrated or the current cabinet calibrated on a previous occasion and the file saved it can now be retrieved for use again If not then the default file default cfg should be used double click on the appropriate text
37. ampshire SP10 5AA United Kingdom STD9000 002 Revision 2 Page 10 of 64 Tele 44 0 1264 835800 Fax 44 0 1264 835801 Email enquiries bioquell com Web www bioquell com 4 INSTALLATION WARNING INSTALLATION SHOULD ONLY BE CARRIED OUT BY TRAINED AND APPROVED ENGINEERS OR AGENTS BIOQUELL OR ITS AGENTS CANNOT ACCEPT RESPONSIBILITY FOR DAMAGE LOSS OR INJURY CAUSED BY OR RESULTING FROM INCORRECTLY INSTALLED EQUIPMENT WARNING DO NOT USE THE PEROXIDE BLOCK OR PIPES FOR LIFTING 4 1 POSITION OF THE CABINET The correct functioning of a Safety Cabinet can only be achieved if the Cabinet is properly installed The position of the Cabinet in the Laboratory is important Advice on the siting of Cabinets is given in The Code of Practice for Prevention of Infections in Clinical Laboratories and Post Mortem Rooms published by HMSO and BS EN 12469 and BS5726 Part 2 1992 The following points should be observed in selecting the position for the Cabinet a Is the room of adequate size About 25 cubic meters 900 cubic feet is generally considered to be the minimum unless special provision is made for make up air b Is the make up air adequate c Will the Cabinet be subject to cross movements of air generated either by open windows the ventilation system exhausts from re circulation cabinets or doors being opened and closed near the Cabinet d Is there space behind the operator movement of o
38. binet for cleaning the inside surface of the window or for loading equipment into the cabinet With the window frame hinged out the window itself can be raised higher To hinge open the widow pull outwards the window frame until it stops then let it back slowly the window will then hold open To shut it again pull the window frame fully open again and it will unlatch allowing it to close Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 17 of 64 Web www bioquell com Figure 6 Window hinged open for increased access and for cleaning Caution Care must be taken to avoid impact damage to the bottom edge of the Front Sliding Window as cracking of the glass may result When wishing to leave the cabinet the fan can be switched off by pressing the fan button or the window can be closed which will also turn off the fan 5 2 1 Integral Bypass Operation The Integral Bypass if fitted works in the same way as described above but also works the room cabinet change over damper as described below Power to the cabinet must be ON If it is switched off or there is a power failure the integral bypass will always shut off the cabinet taking air from the room To start the MSC the user should follow the procedure below e Enable the damper by pressing the fan button Ne e Damper will start to open
39. computer s fixed disc Thus the drive name and directory path should prefix the file name e g C SETUPS FILENAME CFG SETUPS is a directory specifically created for the storage of configuration files and FILENAME is a name for the file which facilitates easy identification e g BS 12 10 5 1 3 Exit The Exit option can be used as an alternative to the close window button to terminate running of the set up program 10 5 2 PIN Nos Maintenance of the PIN numbers can be carried out by selecting PIN Nos The PIN numbers are the four digit codes which allow the fan selections to be locked and also allows use of the vapourisation cycle to those persons with knowledge of the applicable number When the Connect operation has been executed the following two options are available Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 47 of 64 Web www bioquell com 10 5 2 1 Read Allows the stored codes to be interrogated and displayed in a box on the screen 10 5 2 2 Reset Resets the two codes to default values The default values will be displayed but for reference they are Fan functions locking code 1111 Vapouriser function access code 2222 The configuration of different codes must be carried out from the controller keypad once communication between controller and computer has been bro
40. damage 9 4 PAINTED AND OTHER COMPONENTS Caution Do not use water around electrical connections switches etc To clean all painted and other components use a damp cloth and when required a liquid detergent Note Do not use abrasive cleaners Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 40 of 64 Web www bioquell com 10 CONTROL SYSTEM CUSTOMISATION AND CALIBRATION It is strongly recommended that BIOQUELL or a trained agent of BIOQUELL is used to customise and calibrate the cabinet To do this work a PC with appropriate operating system is required as well as the set up software which is available from BIOQUELL 10 1 INTRODUCTION The microprocessor based control system used in the cabinet allows the user two main advantages over the conventional analogue system These are a Cabinet functions can be customised to the user s requirements b Calibration can be performed remote from the cabinet itself without the need to access electrical components located in a potentially hazardous compartment Executions of both of these tasks are made simple and quick with the use of dedicated set up software obtainable form BIOQUELL 10 2 COMPUTER AND CABLE REQUIREMENTS Equipment required to run the set up software comprises a communication cable and a Personal Computer PC with
41. designed to give many years trouble free efficient service and to keep maintenance to a minimum However to ensure this they must be regularly cleaned and checked A full maintenance service is offered by BIOQUELL Overseas this same service is offered by the BIOQUELL distributors In addition to this however operators should carry out the following procedures on a schedule agreed with the Safety Officer so as to ensure that the cabinet is always in first class working order 8 1 DAILY FREQUENTLY a Keep the unit clean externally as well as internally It should be wiped over with a damp cloth using a proprietary bactericidal solution The front viewing panel should be kept clean so that the operator always has a clear view of work being performed inside the cabinet b Ensure that the audible alarm and all indicators are functioning by pressing the TEST button Any faults should be corrected before the cabinet is used 8 2 WEEKLY MONTHLY In addition to the above the following checks should be carried out a Visually check for damage to the seal on the front of the cabinet which is compressed by the glass of the front window b Inspect all mains cables to the cabinet or to any extras that may have been fitted for damage or wear In the event of any fault being discovered contact a BIOQUELL approved service company 8 3 ANNUAL MAINTENANCE TESTING AND RE CALIBRATIONS It is recommended that the annual service vis
42. dual staining of stainless steel To avoid this cleaning agents which containing Chlorine ought to be avoided however if this is not possible then all surfaces should be thoroughly dried and all traces of the solution removed the area should then be treated with a neutralising agent Open vessels left inside the equipment which are likely to cause Chlorine vapours may also result in staining of the metal surfaces Should the surface of the stainless steel become stained it may be cleaned by mechanical polishing and special treatment of the affected area Note When cleaning to remove bio organisms the method we recommend is to wash with 4096 ethanol or equivalent in all poor access points e g corner of liners fold and recesses in the tray It is important to ensure that full surface contact is achieved and the use of cotton buds is recommended 9 3 PLASTIC COMPONENTS Cleaning of the plastic items should only be carried out with mild detergent or chlorine based cleaning solutions Care must be taken not to apply chlorine solutions to any stainless steel metalwork as this will potentially cause staining Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 39 of 64 Web www bioquell com Do not apply heat abrasive materials solvents or solvent wipes to the surface as this can cause irreparable
43. e 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 30 of 64 Web www bioquell com Ensure that the ducting to expel the Formalin is secure to the exhaust plenum and that any manual valves are open Add the formaldehyde solution to the deionised water This must be carried out in a fume cupboard or a Class I safety cabinet ducted outside Remove the cap of the vaporiser add the diluted formaldehyde solution and replace the cap Press the VAP switch and enter 2222 PIN number If the Cabinet is a Peroxide version then the following will be seen 1 Clarus 2 Vaporiser Choose 2 Close the cabinet window and lock the T handles shut The vaporiser operates automatically and takes approximately 30 minutes to evaporate the contents The sterilising cycle is set at 240 minutes After this time the cycle changes to Purging set for 480 minutes When the fumigation cycle is complete the display will show the following alternating messages Fumigation cycle Purging required and Please open Class II window The operator ought to open the window or if a Formaldehyde Extraction Unit is being used switch it on and after 10 to 15 seconds open the orange admission valve on top of the cabinet and allow the unit to run for the purging time Periodically check the room with a formaldehyde meter this should read less than 2 p
44. e downflow should fall outside this range the cabinet will alarm The cabinet is designed to have approximately 7096 of the flow re circulating and 3096 exhausted The airflow patterns are shown below in figure 1 Note a single exhaust filter is shown it is possible to have a double exhaust when re circulating the air into the room this is fitted with a fan in the top box 8 WA NANI Figure 1 Airflow patterns 2 1 RESPONSIBILITY OF THE USER It is not possible to detail all the Safety requirements for every procedure undertaken inside a Safety Cabinet It is the responsibility of the user to ensure safe and proper techniques Contact BIOQUELL for advice if required Bioquell TM003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 6 of 64 Web www bioquell com 3 SPECIFICATION 3 1 OPERATING ENVIRONMENT The cabinet will operate satisfactorily in environments where the ambient temperature lies between 10 and 35 C and the relative humidity does not rise above 60 Condensation of moisture on the cabinet must be avoided at all times as this will affect filter efficiency 3 2 CONSTRUCTION The outer shell is of mild steel sheet finished in white powder coating The work area is made from 316 stainless steel 3 3 DIMENSIONS WEIGHT 1 0m 1 2 m 1 5 m 1 8m CABINET CABINET CABIN
45. ed on Certificate of Factory Testing complies with the Protection Requirements of the Electromagnetic Compatibility Directive 89 336 EEC and the Low Voltage Directive 73 23 EEC by means of the following harmonised standards EN 61326 1997 EN61000 3 2 1995 amp 2000 1000 3 3 1995 Tested By EMC Projects Ltd Holly Grove Farm Verwood Road Ashley Ringwood Hants BH24 2DB Certificate No 5790 04 EN 61010 2001 BS EN 12469 2000 Responsible Person Name O R Cumberlege Position Director Signed 2 Date 20 December 2004 Bioquell Walworth Road Hampshire SP10 5AA United Kingdom TM003 9900 Tele 44 0 1264 835800 Revision 1 Fax 44 0 1264 835801 Email enquiries bioquell com Page 60 of 64 Web www bioquell com 16 APPROVED SERVICE COMPANY We only recommend BIOQUELL in the UK or its local service provider to carry out service and repair work These technicians have been fully trained on the product To contact BIOQUELL use one of the following methods e Call the Service Desk on 01264 835 800 or 0800 527800 e Faxto 01264 835 801 e Email to enquiries bioquell com For details of our local service providers outside the UK contact BIOQUELL on 44 1264 835 800 Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 61 of 64 Web www bioquell co
46. g plate fitted to the top box A notice should be attached to the cabinet to indicate that the fumigation cycle is operating THE CYCLE MUST NOT BE STOPPED The fumigation cycle will not commence unless the window is detected as being locked Ensure that the cabinet is free of hydrochloric acid and other chemicals that may contain chlorines Remove the cap of the vaporiser add the diluted formaldehyde solution and replace the cap Press the VAP switch and enter 2222 PIN number The cabinet will then display Fumigation Cycle No X X counts from 1 to 10 If run again after the 10th cycle the following message will be displayed Maximum cycles for filter reached and the unit will not run any further cycles until the filter has been changed and the counter reset see section 7 2 2 2 Evaporating X minutes to run will be displayed The vaporiser operates automatically and runs for the set time X to evaporate the contents A warning is displayed not to open the window The unit will then progress into fumigating with displaying the time to run and repeating the warning not to open the window Finally the purging stage starts with a repeat not to open the window on the display This is where the vapor will be automatically circulated through the filter where the formalin vapor is scrubbed out When the cabinet has finished the cycle it displays the following message Fumigation cycle No X complete where X is the number
47. hat a hot wire anemometer is used as this gives the most accurate reading however a 100mm diameter paddle wheel anemometer can also be used Only re calibrate if any parameters or alarms need altering 11 3 2 Inflow Velocity The inflow velocity shall be derived from a measurement of the exhaust airflow and a consideration of the ratio of exhaust to inflow aperture areas Proceed by either using an existing installed calibrated airflow devise in the duct Alternatively take five exhaust flow measurements each for at least 1 minute with a hot wire anemometer head positioned in the centre of the exhaust area and the centre of each of the quarters of this area The velocity of inflow air is then calculated using the following equation V us X V a As where variables are defined V velocity of inflow air in m s V average value of five exhaust velocity measurements in m s A area of exhaust aperture in m A area of inlet aperture in m Where it is not possible to access the duct to measure the airflow or the cabinet is a re circulatory type then the airflow can be measured through the front aperture A 100mm diameter paddle wheel ought to be placed in the position shown in figure 18 Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 51 of 64 Web www bioquell com Ee Figure 18
48. he Contract shall not exceed the price of the Goods Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 63 of 64 Web www bioquell com Note When requesting a Warranty visit please have the following information available i Product model number and name ii Serial number iii Date of last service and Service Company iv Nature of fault and any other comments likely to indicate cause of fault v A Purchase Order number to cover costs incurred if visit is outside the scope of the Warranty BIOQUELL UK Ltd or other nominated personnel will carry out warranty visits g In the event of any health and safety incidents please advise us in writing at the earliest opportunity h This warranty and all other contractual issues shall be governed by English law and the parties agree to submit to the nonexclusive jurisdiction of the courts of England Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 64 of 64 Web www bioquell com
49. he top Figure 5 The cabinet will start to alarm with the following message WARNING LH Unlatched and WARNING RH Unlatched Figure 4 Figure 5 Window frame closed Window Frame released Red dot at bottom Red dot at top With both sides released the glass window can be moved using the handles on the glass up to the operating position As the window is raised the fan s will start to operate When the glass reaches the operating position a stop will prevent it from being raised any further in addition there is a label indicating where the bottom of the glass ought to be Then push the window frame inwards and turn the two T handles clockwise half a turn to re secure the window onto its seal figure 4 If the window is not at the correct height or the two T handles have not located correctly the following alarm messages will continue to be displayed WARNING LH Unlatched and WARNING RH Unlatched The fan will take approximately a minute to stabilise and will display STABILISING FLOW then the following message is displayed CLASS Il SAFE stating that the cabinet is safe If the fan does not operate press the Fan button on the keypad Ka To close the window carry out the same procedure in reverse The cabinet will alarm when the T handles are released and the fan will stop when the window is shut The Front Sliding Window may also be hinged Figure 6 allowing access into the ca
50. ig 16 Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 48 of 64 Web www bioquell com 10 6 MISCELLANEOUS FUNCTIONS 10 6 1 Dis Connect This button serves both the functions of connecting and disconnecting the communication link between the PC and Controller The current status of the communication link between the PC and the controller is indicated by the graphic on the Dis connect button When the communication link is disconnected the two connectors are shown separated when connected they are together 10 6 2 Read Although all setup parameters are automatically read from the controller and displayed on the screen whenever a Connection is made Read will repeat this function Read is therefore used after a variable has been changed and written to controller memory for example and it is subsequently required to check the controller s current settings 10 6 3 Calibrate The calibrate button initiates the calibration cycle which is explained in Section 11 Calibration of Airflow and Alarms 10 6 4 Clear all Clicking on the Clear all button will reset all fields on the setup screen to their default values Commonly default values are zero 10 6 5 Write The Write function will load all current screen parameters to the controller memory Bioquell STD9000 002 Tele 44
51. in 3a to 3c are changed by clicking in the appropriate box and then typing the required time The time entered is in minutes for which the device is active Values can range between 0 and 255 minutes for the mute timer and 0 to 999 minutes for other parameters 3a Overrun timer Pressing the FAN DELAY OFF button causes the fan to continue running for the time period entered 3b UV timer Pressing the UV button switches the UV lamp on for the time period entered 3c Mute timer Pressing the MUTE button mutes the buzzer for the time period entered 4 Vapouriser Cycle The vapourisation cycle consists of sequential phases with configurable durations of between 0 and 999 minutes To change the settings after Connecting click in the relevant box and edit as required If these are the only operations to be carried out after amending the values click on Write followed by Disconnect 4a On time This is the time at the start of the cycle when the vapouriser heater is powered Choose a suitable time to achieve evaporation of all of the fumigant solution but not to run the heater needlessly A thermostat will break and reset power to the unit if the evaporation temperature is exceeded As a guide if 22 ml of approximately 4096 solution with an equal volume of water is being used then 26 minutes should be an appropriate value for this timer 4b Fumigation time This is the time required for the fumigant to sterilise the cabinet
52. ing status of the product removing BIOQUELL s Duty of Care and responsibility c Consumables such as pre filters HEPA filters light bulbs and tubes not warranted d This Warranty is void if faults are caused by accidental damage mishandling adjustment by unauthorised personnel or failure to follow the correct maintenance and safety precautions as stated in the Instruction Manual e The Warranty expressly provided for herein is the sole Warranty provided in connection with the product and no other Warranty expressed or implied is provided BIOQUELL UK Ltd assumes no responsibility for any other claims consequential including lost time or profit or other damage whether based in contract tort or otherwise not specifically stated in this Warranty f Except in respect of death or personal injury caused by Seller s negligence or as expressly provided in these Conditions Seller shall not be liable to Buyer by reason of any representation unless fraudulent or any implied warranty condition or other term or any duty at common law or under the express terms of the Contract for any loss of profit or any indirect special or consequential loss damage costs expenses or other claims whether caused by the negligence of Seller its servants or agents or otherwise which arise out of or in connection with the supply of the Goods or their use or resale by Buyer and the entire liability of Seller under or in connection with t
53. internal surfaces and all installed components Thus choice of an appropriate value will depend on the sterilising agent its concentration and the nature of the source of contamination within the cabinet A figure of 240 minutes is suggested as a suitable value assuming 4096 solution Formaldehyde is being used 4c Purge time This is the time period following fumigation when the fan operates to extract the sterilising agent out of the cabinet Again careful consideration needs to be given to the choice of this value since an adequate time needs to be Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 44 of 64 Web www bioquell com allowed to ensure that all traces of the material have been removed The following points should be remembered i Although the OES Occupational Exposure Standard may be satisfied and the presence of the fumigant is not detected by smell the working area of the cabinet may still constitute a hazardous environment towards the material being processed ii X Trials with the cabinet have found that concentration levels of fumigant within the cabinet when subjected to purging action exhaust ducted outside laboratory drop rapidly initially but a disproportionate time is required for final reductions to the acceptable level iii A value for this time of 240 minu
54. it is carried out by BIOQUELL or its local distributor The service shall consist of the following a Visually check the cabinet internally and externally for surface defects cracks or other damage Check the window and its operation and seals b Extract duct work to be examined where practicable for defects cracks or other damage if fitted and that it is clearly labelled C Check the anti blow back if fitted is functioning and clearly visible O Bioquell TM003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 36 of 64 Web www bioquell com d All alarms to be checked and tested and adjust the downflow and exhaust if required e Filter and seal integrity checked using DOP If there are two exhaust filters they ought to be checked independently f Downflow and air inlet velocity g Pre filter changed if fitted h Full function and keypad test i KI discuss test is recommended 8 4 FILTER SERVICING WARNING FILTERS MUST BE DECONTAMINATED PRIOR TO REMOVAL WHEN REMOVED THEY MUST BE BAGGED AND DISPOSED OF IN A SAFE MANNER It is recommended that this done by a trained service provided If it is necessary to change the filters the following procedure ought to be followed When changing filters it is important that both main and exhaust filters are replaced at the same time In this way the resista
55. ity is to be broken for any other reason Or there is a workspace decontamination cycle which can be used to decontaminate the working area and under the work tray This is a suitable procedure when general servicing of the cabinet is to be done or a bio decontamination is required between experiments to stop cross contamination The advantage of the workspace only decontamination is that it is significantly shorter Running a Filter Decontamination Cycle a Run a decontamination cycle with the Clarus according to the Clarus manual Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 26 of 64 Web www bioquell com The following cycle parameters ought to be set for bio decontamination of the filters Conditioning Time 10 minutes Dwell Time 15 minutes Aeration Time 100 minutes H202 Volume 100 ml for 1 0 amp 1 2m and 125ml for 1 5 amp 1 8m Pressure S P 10Pa Pressure Low 80Pa Pressure High OPa Pressure Enable ON Aeration Pressure Enable OFF Remote Start OFF Pressure PID parameter set If it is not possible to purge using the duct or vent pipe set to ON Select the Vapor key 7 and enter the correct PIN default 2222 the screen will display 1 Clarus 2 Vaporiser Press key 1 to select the Clarus on the cabinet and then start the cycle on the Clarus L The Cabinet will display
56. ken To break the link click on the Dis connect button Note The current status of the communication link between the PC and the controller is indicated by the graphic on the Dis Connect button When the communication link is disconnected the two connectors are shown separated when connected they are together The status of the communication link should not be confused with physical condition of whether the cable is connected or not e g the cable may be connected but the communication link may not be made 10 5 3 Comm Port The Comm Port menu options allow the selection of either COMI or COM2 serial ports on the computer to be used for communication to the controller By default COM2 will be selected but the assignment can be changed to COMI by clicking on the appropriate text If a portable computer is used for setting up the use of COMI is likely to be the only serial port available for external connections Thus it will be necessary to make this selection before connection to the controller can be made 10 5 4 Options Selecting Options allows enabling of the following two facilities 10 5 4 1 Direct Control When selected the fan speed sliders area 9 of Fig 16 can be used to directly adjust the Main and Exhaust fan speeds This is useful primarily during the calibration process described in the next section 10 5 4 2 Show Sensors When selected numerically scaled sensor output values are displayed in area 7 of F
57. l or external shor fan to operate at full speed This facility is intended for emergency use after a spillage or other inadvertent activity A second press of this button will switch the cabinet s internal or exhaust fan off Alternatively either of the other two fan buttons can be pressed in order to select the desired action 5 1 6 Alarm Mute Em When an alarm condition occurs the buzzer will sound Pressing X this button will cause the buzzer to be muted for a configurable time period of between 0 and 255 minutes See Section 10 Control System Customisation and Calibration The default setting is 4 minutes Cabinets to BS EN 12469 require the default setting at O minutes The intermittent beeping that accompanies FLOW STABILISATION EXPIRED SERVICE DATE messages or attempts to select incompatible conditions are not muted 5 1 7 Test Correct operation of button indicators and the audible alarm may LA be checked by pressing the TEST button all indicators should 5 light and the buzzer should sound with a continuous tone The TEST function serves the secondary purpose of allowing the HOURS RUN and SERVICE DATE DUE information to be displayed This data is useful in the planning of maintenance operations 5 1 8 Fluorescent Lights 779 Pressing this button turns on the cabinet s fluorescent lighting Xx With power connected to the cabinet the lights can be switched on
58. le tab in and pull the drawer up and out The two fuses are attached to the drawer Replace always with T10 Amp fuses Changing of any fuses on the control board must be carried out by a trained technician Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 38 of 64 Web www bioquell com 9 CARE AND CLEANING 9 1 BIO ORGANISM REMOVAL CLEANING Special attention for cleaning must be given to the door seal Thoroughly clean with disinfectant then rinse well with sterile water All MSCs should be kept clean and free of unnecessary equipment The interior should be swabbed after use with a suitable disinfectant Phenolics quarternary ammonium compounds and aldehydes can be use for disinfecting these surfaces Users of chlorine compounds should be aware of their corrosive nature The use of alcohols should be undertaken with caution because of the risk of fire Heavy duty polyvinyl chloride PVC or rubber gloves which provide suitable protection for the hands and wrists which can be disinfected for re use should be worn when cleaning MSCs At the selection of disinfectants occupational health aspects should be considered 9 2 STAINLESS STEEL COMPONENTS The internal work area s sides back and work tray are made of stainless steel Liquids or vapours containing Chlorine are known to cause gra
59. m 17 SAFETY SYMBOLS BIOHAZARD CAUTION FAN Zye tOO eee EARTH GROUND TERMINAL CAUTION RISK OF ELECTRIC SHOCK PROTECTIVE CONDUCTOR TERMINAL POWER ON OFF Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 62 of 64 Web www bioquell com 18 WARRANTY BIOQUELL UK Ltd produces products that are warranted under normal usage against defects in workmanship and materials for one year parts and labour costs from the date of manufacture The Warranty is stated in the Standard Terms and Conditions of sale Export and Agent retailed products are warranted directly by the Agent Please confirm your warranty and liability status with the Agent In addition the Warranty is void unless the following conditions are met a The product has been installed and used as stated within the Instruction Manual b The warranty does NOT include servicing or maintenance An approved service company who have attended our training courses for your product must carry out maintenance of product Failure to maintain or service this product will invalidate the warranty Maintenance must be carried out in accordance with the Service Manual and include tasks within stated periods Failure to use approved service companies or BIOQUELL UK Ltd trained personnel for maintenance also affects the CE Mark
60. m Page 4 of 64 Web www bioquell com 1 FOREWARD This manual has been specially prepared to give guidance in the use and maintenance of the MICROFLOW CLASS II ADVANCED BIO SAFETY CABINET ABS Mk II WARNINGS 1 USE OF THE CABINET IN A WAY OTHER THAN DESCRIBED IN THIS MANUAL COULD PROVE TO BE VERY DANGEROUS 2 GERMICIDAL LAMP EMITS UV RADIATION 253 7 nm EXPOSURE WILL CAUSE DAMAGE TO SKIN AND EYES VACATE AREA WHEN IN USE A list Safety Symbols used on the Cabinet labels is provided in Section 17 In order to utilise the full operator protection factors given by the cabinet it is essential that the operator is fully familiar with the cabinet and all of its functions and controls This manual should be thoroughly studied before the cabinet is used This manual contains the operational and first line maintenance instructions Service and maintenance operations other than those covered by this manual should only be undertaken by BIOQUELL s recommended service agent see Section 16 Care should be taken to follow the instructions in this manual to ensure safe operation However if further clarification is required contact your supplier 1 1 SAFETY ENVIRONMENTAL CONDITIONS The cabinet is for indoor use only For use at altitudes up to 2000 m The unit is safe to run electrically between 5 C and 40 C at 10 to 80 humidity however the unit ought not to be operated outside a temperature range of 10 to 35 C and
61. mit Inflow low gas solenoid If front screen open and left hand side unlatched Warning screen LH unlatched Warning only If front screen open and right hand side unlatched Warning screen RH unlatched Warning only If the speed of the recirculation fan required to maintain the downflow airflow at the calibrated setpoint is greater than 80 6 of full speed Warning replace filters Warning only If fan ventilation enabled and the front screen is locked shut Warning screen locked shut Warning only If fumigation cycle aborted Fumigation cycle ABORTED Warning only If fan ventilation enabled and the mask time expired and the downflow or exhaust airflow sensor not connected or greater than maximum limit Warning Flow sensor failure Disables optional gas solenoid If screen right hand side latched in lower position and the left hand side unlatched Warning Screen shut LH unlocked Warning only If fan ventilation not enabled and the front screen window not shut and latched in the lower position Fan off Window open Warning only If service due date exceeded Service overdue Warning only If battery backed data not retained after power failure Note this would denote a loss of all calibration setpoints Fan 100 Battery Fail Fan runs at full speed All alarm warning events will be displayed on the LCD as a text message The red
62. months 6 months 9 months 12 months C Disable No change dm v2 02 f s ri 166 Figure 20 Typical Alarm Calibration Screen Main Fan Speed 4 2562 Cancel Exhaust Speed A h When the correct speed has been obtained as indicated on the anemometer click on the Continue button Although the software waits until sensor values are stable it is advisable to observe the sensor values reference 7 in Fig 16 until the numbers are steady before clicking Continue Bioquell Walworth Road Hampshire SP10 5AA Email enquiries bioquell com United Kingdom STD9000 002 Tele 44 0 1264 835800 Revision 2 Fax 44 0 1264 835801 Page 54 of 64 Web www bioquell com i Repeat steps g and h as instructed This will enable the calibration point to be established for the high downflow alarm A similar repetition for calibration of the inflow alarm can next be performed The screen shown in figure 21 will be seen NOTE do not follow the instructions on this screen but follow the procedure given in 11 3 2 FA Class II Bsc Setup Program 2 44 File PIN nos CommP tions Messages Languages Serial No eal xi Position anemometer at the average inflow 17 05 2005 11 18 position and close the front screen down to B the anemometer J Adjustthe exhaust fan speed slider until the J
63. n then be connected to the socket labelled UV on the internal wall of the cabinet Two safety related interlocks are incorporated into the circuit powering the UV lamp The front viewing panel must be fully closed thereby achieving the alignment of a magnet on the window with a reed switch in the rear of the lamp casing If alignment of the two components is not achieved the lamp will not function A series of holes on the rear of the lamp casing indicate the position of the switch if the UV light does not work when expected adjust its position slightly The fluorescent lights must be switched off Unless these lamps are off it will not be possible to switch on the UV lamp The U V light is operated by use of the U V light switch see section 5 1 9 6 11 3 Remote Start Stop Switch A remote start stop switch can be supplied to be fitted outside the room where the cabinet is fitted This key switch requires turning momentarily to initiate the stopping of the fan and starting it replicating the fan button Note that the window on the cabinet must be in operating position otherwise the cabinet will alarm and not operate Note if an Integral Bypass is fitted the Fan time delay button must be used to turn off the fan at the cabinet as this allows the window to be left open without the alarm sounding If turned off at the remote keyswitch the alarm will not sound either Bioquell STD9000 002 Tele 44 0 1264 835800
64. nce of the filters and therefore the proportion of downflow to inflow will remain constant Failure to change both will be likely to upset the balance and therefore affect the protection factors 8 4 1 Exhaust filter s WARNING The CABINET MUST BE SWITCHED OFF AND DISCONNECTED FROM THE POWER SUPPLY The four nuts holding down the top box or exhaust plate which is located on the top of the cabinet must be removed Lift off or hold up the top box or exhaust plate Remove the screw at either side of the cabinet which holds the front cover down Then lift the front cover and two teleprops on either side will hold it open Lower the front sliding window to the closed position Remove the metal cover plate which is held with a series of screws The exhaust filter s are the smaller filters located towards the top of the unit Lift the top of the ductwork inside of the cabinet and remove the filter After replacing the filter put the top box back on and by clamping it down the exhaust filter will be sealed in position Replace the metal cover plate and lower the front cover by pushing it up allowing the tele prop to unlatch Secure the front cover with the two side screws Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 37 of 64 Web www bioquell com 8 4 2 Downflow filter Follow the
65. ocket the same procedure can be followed as described below but the vaporiser unit will have to be turned on manually A safe manner to exhaust the fumigant is required the following are a list of common methods Use the existing Ducting for permanently ducted cabinets Fit temporary ducting to a central duct or other exhaust system Fit a Formalin Evacuation unit to the exhaust a small portable unit designed specifically for this purpose available from BIOQUELL Use an in built filter for removing the vapor only fitted to Option K versions of the cabinet refer to section 7 2 2 The cabinet display prompts the user throughout the fumigation cycle A safety feature built into the control system is that if the Window is disturbed during the evaporation or fumigating processes the fan will automatically be switched on in order to safely expel the fumigant up the duct work or through the Evacuation unit It is therefore most important the extract duct work is fitted prior to the start of the cycle In such a situation the fan will continue to run until the Keylock button is pressed and the fumigation PIN number has been entered for a second time The recommended quantities of the solutions used in the following procedures are SOLUTION 1 0m 1 2m 1 5 m 1 8 m CABINET CABINET CABINET CABINET Formaldehyde 17 22 26 30 Solution 40 w v ml De lonised Water ml 17 22 26 30 Bioquell STD9000 002 Tel
66. of the downflow upper and exhaust lower sensor output values The numbers displayed between 0 and 255 proportionally relate although not linearly to the airflow over the respective sensor Thus observation of the values can be of assistance during calibration diagnosis of faults and commissioning Note As a default Show sensors is selected Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 45 of 64 Web www bioquell com 8 Setpoint Adjustment This slider allows the controlled operating point of the cabinet fan s speed to be adjusted once calibration has been performed The operating point always defaults half way between the high and low alarm points Since this facility is related to the calibration process a fuller description can be found in Section 12 2 Setpoint Adjustment 9 Fan Speed Adjustment These two controls allow the speeds of the main and exhaust fans to be adjusted 9a Main Fan Speed Manual speed control of the cabinet s main fan can be achieved by use of the slider labelled 9a in Fig 16 Manual adjustments may be necessary when a specific flow is required in the cabinet such as checking or setting the low and high downflow alarms To use the manual speed adjustment the software should firstly be Connected then manual control can be enabled by clicking on Op
67. om United Kingdom Page 28 of 64 Web www bioquell com Aeration Time 60 minutes H202 Volume 25 ml Pressure S P 10Pa Pressure Low 80Pa Pressure High OPa Pressure Enable ON Aeration Pressure Enable OFF Remote Start OFF Pressure PID parameter set If it is not possible to purge using the duct or vent pipe set to ON b With the window locked down start the Clarus L cycle There is no need to run the Cabinet s Decontamination cycle as well After 30 minutes the Clarus L will be starting Aeration it is highly desirable to exhaust the peroxide through the duct work or temporary vent to atmosphere as the time taken to purge will be dramatically reduced CHECK WITH THE HEALTH AND SAFETY OFFICER FIRST THERE WILL BE HIGH LEVELS OF HYDROGEN PEROXIDE IMMIDIATELY LOCAL TO THE EXHAUST OUTLET To purge up the ductwork or temporary vent Open the cabinet s window to its normal working height slowly and lock in position When the window is opened the fan will come on immediately WARNING THERE WILL BE HIGH LEVELS OF HYDROGEN PEROXIDE IN THE CABINET CARE MUST BE TAKEN WHILE THE FAN IS STARTING AS SOME PEROXIDE MAY EXIT THE WORKSPACE DO NOT FULLY OPEN THE WINDOW OR INHALE AIR FROM THE WORKSPACE UNTIL SAFE LEVELS OF PEROXIDE HAVE BEEN ESTABLISHED When the cycle on the Clarus L has completed check the level of Hydrogen Peroxide in the cabinet by independent means if below 1ppm the cabinet is safe to use No exte
68. on the label and figure 2 It is the area of the lower flat part of the work tray up to 100mm below the filter Figure 2 Safe working area 3 6 NON RETURN VALVES A Non Return Valve is located after the HEPA exhaust HEPA filter s in the top box plenum This is to stop blow back and may not be 100 gas tight but there will be no leakage of vapour if operated at negative pressure when being de contaminated The top box may be turned so that the exhaust ductwork may be taken either from the right or left hand side Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 8 of 64 Web www bioquell com 3 7 ALARMS Safety has been considered the primary factor in the design of this cabinet Consistent with this an alarm condition will be indicated when the following conditions occur Alarm warning event Text message Action If fan ventilation enabled and the mask time expired and the downflow Warning Disables optional airflow greater than calibrated limit downflow high gas solenoid If fan ventilation enabled and the mask time expired and the downflow Warning Disables optional airflow less than calibrated limit downflow low gas solenoid If fan ventilation enabled and the mask time expired and the inflow Warning Disables optional airflow less than calibrated li
69. ontaminate it with a simple site modification a Check that the cabinet is running in SAFE MODE b Release the window locks and pull the window down the fans will stop working then lock the window shut by use of the two T handles on either side of the window c If the unit is Ducted ensure that it is safe to vent Hydrogen Peroxide through the duct work If the unit is a re circulatory cabinet then attach a vent pipe to the exhaust spigot for safe external ventilation or otherwise the Clarus can Aerate on its own although this may take a long time d Connect the hoses colour coded red supply and blue return and the pressure tube to the connectors on the rear of the Clarus L as shown in Figure 12 Bioquell TM003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 25 of 64 Web www bioquell com Connect the other end of the hoses and the pressure tube to the corresponding connectors on the right hand side of the Cabinet as shown in Figure 13 The blue hose connects into the top connection and the red in the bottom with the pressure tube connected in the centre Figure 13 Cabinet Hose and Pressure Tube Connection There are two different types of cycles which are available either a filter decontamination cycle which ought to be used if the filters are going to be changed or the filter integr
70. pm formaldehyde Purging will continue until the purge time has expired The cabinet will then return to normal cabinet functions Press the Fan button and the airflow will stabilise to allow safe operation Remove the temporary duct Evacuation unit close the orange valve first as appropriate and run the Cabinet as normal and check the room with a Formaldehyde Meter A description of the cycle is provided in Table 1 Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 31 of 64 Web www bioquell com Table 1 Fumigation Ducted Cycle ACTION PROCESS DISPLAY MESSAGE Lock shut window Fit Start Cycle Enter PIN Number blanking plate and pour formalin into vaporiser Press VAP Enter four digit Number Vaporiser powered to Evaporation xx mins to run evaporate formalin turn on manually if unit is not integral Vaporiser powered for xx minutes Formalin sterilises inside Fumigating xx mins to run of the cabinet Fan circulates fumigant for Circulating one 5 second phases Fumigation completed Alternating messages Fumigation cycle Purging required and Please open Class II window Open Window Fan will start to purge Purging xx mins to run fumigant from cabinet When purge time is expired the cabinet will resume normal operation
71. procedure given in 8 4 1 to remove the exhaust filter Once the exhaust filter has been removed next remove the plenum which is the part over the large downflow filter this is done by Lift the back of the plenum up by about 30mm 1 and push back The back of the plenum will now be supported on two brackets at the back Then lift the front of the plenum up and hold it by placing the hook on the wire located inside at the top through the eye attached to the plenum Then slide out the filter Once the filters have been changed the cabinet filters require retesting DOP and re commissioning 8 5 FLUORESCENT TUBE REPLACEMENT WARNING The CABINET MUST BE SWITCHED OFF AND DISCONNECTED FROM THE POWER SUPPLY Remove the screw at either side of the cabinet which holds the front cover down Then lift the front cover and two teleprops on either side will hold it open Remove the ends off the tube by simply pulling away and then unclip the tube from the holders Place the new tube in the holders place the end on and fit down and secure the front cover 8 6 UV TUBE REPLACEMENT To change the tube pull off the end fittings unclip the the tube from the the holding clips Place the new tube in the holder by doing the above in reverse order 8 7 CIRCUIT PROTECTION There are two fuses located next to the power entry socket Note the 120V version does not have any fuses but an overload trip instead To gain access to the fuses push the litt
72. quell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 22 of 64 Web www bioquell com Parameters which affect the closing of relay contacts are a Cabinet fan on b Airflow alarm active Contacts are rated at maximum 1 A at 24 V a c or d c Refer to Fig 11 for connection details DESIGNATION CONNECTION ALARM A N O Airflow Alarm On F OA B N C O O C Common E B D N O Cabinet Fan On O DO E N C F Common Figure 11 Volt Free Contact Wiring 6 10 INTERNAL SOCKET RESIDUAL CURRENT DEVICE As an additional operator safety feature a Residual Current Device RCD is available and when fitted is located adjacent to the circuit breaker and main ON OFF switch on top of the cabinet see figure 8 The device will isolate power to the socket if a difference in currents flowing along live and neutral conductors exceeds 30 mA To set or reset the RCD push the black lever to the On position It is recommended that the RCD is checked for correct operation before each time it is re set To test press the TEST button on the RCD once the black lever has been set to On the black switch should then immediately go to the Off position 6 11 U V LIGHT The U V light is not recommended for use in Safety Cabinets in EN 12469 2000 and is an inappropriate method to de activate category 4 pa
73. rnal Purge If it is not possible to purge externally the Clarus L can carry out the Aeration then the cabinet ought to be left in the same state as for gassing At the end of Aeration by the Clarus L the operator ought to check the level of Hydrogen Peroxide in the cabinet by independent means if below 1 ppm the cabinet is safe to use Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 29 of 64 Web www bioquell com 7 2 DECONTAMINATION WITH FORMALIN Formaldehyde and Formalin are hazardous observe handling procedures Ensure that the cabinet is free of Hydrochloric acid and other Chloro compounds This will avoid the production of the carcinogenic product that results from the mixing of formaldehyde and HCI or Chloride gas The following procedure is provided for guidance purposes only your Safety Officer should be consulted for definitive guidance Formaldehyde penetrates poorly and its effectiveness is dependendent on temperature and humidity It is most effective above a temperature of 20 C and a relative humidity of 65 Use of excessive amounts can result in polymer deposition within the cabinet and may contribute to filter blockage 7 2 1 Fumigation with an Integral or Separate Fumigation Unit If a stand alone separate fumigation unit is used which is then plugged into the internal s
74. roller s memory will remain unchanged n Clicking on Continue will then terminate the calibration process and store all the parameters configured on the setup screen to the controller s memory 0 Calibration is now complete Break the communication link between PC and controller by clicking on the Dis Connect button Note If new setup parameters different from configuration files already stored on the PC s disk are required to be saved for future use this operation should be carried out before Dis Connecting To save a configuration file edit all variables to the figures required on the screen then execute the file interface Finally click on Save For further details see Section 10 5 1 File Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 56 of 64 Web www bioquell com 12 ADDITIONAL AIRFLOW SET UP FUNCTIONS 12 1 EXHAUST FAN SETUP After Calibration the exhaust fan will require setting to an appropriate speed This fan will operate at a constant speed irrespective of changes in airflow The speed is set by use of the slider on the setup screen arrowed 9b in Fig 16 Follow these steps to ensure correct setting a Setup cabinet as described in Section 11 4 Calibration Procedure b Set the exhaust fan slider 9a on Fig 16 to the following value above the alarm point
75. suitable personnel protection equipment consult your Health and Safety Officer Environmental regulation for the disposal of such material may be applicable consult your Health and Safety Officer The new carbon will be supplied with a sealable bag in which the used carbon filter should be put as it is removed from the cabinet 7 3 GASSING THE LABORATORY Should this be required at any time full information for UK organisations is given in the Department of Health Memorandum Control of Outbreaks of Small pox 1975 and advice should also be taken from your Safety Officer Laboratories can be decontaminated using Hydrogen Peroxide with a Clarus generator or using the BIOQUELL Room Bio Decontamination Service RBDS Alternatively Formalin may be used but this methods has a significantly longer down time and associated additional hazards It should be noted that Formaldehyde vapour in normal dilutions will not affect any part of the cabinet or its controls Note It is the responsibility of the user to ensure that whatever means of cabinet decontamination is used it is compatible with the material being handled and has been approved by the Safety Officer Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 35 of 64 Web www bioquell com 8 MAINTENANCE The Biological Safety Cabinets have been
76. ternal or external fan together with the airflow control system to operate Alarms are d muted for a period of about 1 minute during which time the display will show the message STABILISING FLOW If after this time no alarms are active the correct airflow will have been established and the display will show an appropriate message for example CABINET SAFE A second press of the fan button causes the fan s to be switched off Alternatively one of the other fan controls or the vaporisation cycle can be selected to provide the desired action 5 1 4 Fan Off Delay EG Pressing this button when the fan has been running LEDs of oh buttons with numbers 1 or 3 lit will cause the fan to be 2 switched off after expiry of a timed period The delay period is configurable from the set up software See Section 10 Control System Customisation and Calibration Pressing this button a second time cancels the remaining delay time and switches off the fan s Bioquell TM003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 14 of 64 Web www bioquell com Once the fan delay is activated it can alternatively be cancelled by pressing either of the other two fan functions buttons 1 or 3 This will initiate the relevant function 5 1 5 Fan 10096 T Pressing this button will cause the cabinet s interna
77. tes has been found to be appropriate to bring concentration level within the currently published OES value 2 ppm However your Safety Officer should be consulted for definite guidance on this and other timer settings suggested 5 Language The language of messages displayed on the controller screen can be selected by clicking on the appropriate button corresponding to the flag of the nation whose language the messages are required to be displayed 6 Alarms The value at which alarm limits are to be calibrated are entered in the three fields 6a to 6c On execution of a calibration process it is the values entered or previously entered and stored which the user is prompted to set in order that airflow alarms can be recorded by the controller The alarm values are stored as configuration files How to Save and Load configuration files is described in Section 10 5 1 File The fields labelled 6a 6b and 6c refer to alarm values in m s of high downflow low downflow and low inflow respectively To create a configuration file click the pointer in the relevant field press the Delete key as required and enter the desired value When all other desired parameters have been entered select Save as described in Section 10 5 1 2 Save to store the settings on the PC disk 7 Sensor Output Providing the Show sensors option has been selected from the Options menu the fields adjacent to the symbols will display a numerical representation
78. ther staff without disturbing the air flow patterns It is recommended that a smoke test be performed in the laboratory where a Cabinet is to be sited to ensure that there are no adverse movements of air A smoke test should also be conducted after the Cabinet has been installed to check the air movements and room pressure while this Cabinet is running 4 2 POSITION OF THE FAN FOR DUCTED CABINETS The Fan should be positioned at the exhaust end of the ductwork which should terminate outside thus ensuring that internal ductwork is kept under negative pressure Should it be necessary to install the Fan inside the building then the ductwork from the Fan to the outside of the building must be kept as short as possible and never longer than two metres Ductwork should be constructed from a smooth non porous material e g rigid PVC The length of the ducting should be kept as short as possible the fan must Bioquell TMO003 9900 Tele 44 0 1264 835800 Walworth Road Revision 1 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 11 of 64 Web www bioquell com be sized for the duct work and an additional pressure drop from the cabinet The following table gives the pressure drop and the flow volumes the duct work must be designed to Cabinet Min Airflow Normal Airflow Max Airflow Pressure M hr M hr M hr Drop Pa 1 0m Cabinet 180 276 311 380 1 2m C
79. thogens Any surface that is not in contact with the direct UV rays will not be disinfected This will include the rear and sides of the portable UV lamp assembly Therefore when the lamp is removed it must be cleaned using a proprietary disinfectant The UV light transmission from the tube reduces with use and thus the tube ought to be replaced regularly The UV light is operated on a timer of a default of 15 minutes If the exposure to the UV light requires altering this can be easily done through the maintenance software refer to your local distributor or alter with set up software as described in section 10 4 Customisation of Controls Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 23 of 64 Web www bioquell com WARNING DUE TO ITS HAZARDOUS EFFECTS THE CABINET OPERATOR SHOULD AVOID ANY EXPOSURE TO UV RADIATION 6 11 1 High Level U V Light The high level U V light is permanently fitted and is operated by use of the U V light switch see section 5 1 9 As a safety feature the internal fluorescent lighting must be switched off and the window shut prior to switching on the U V light otherwise it will not operate 6 11 2 Portable U V Light Place the Portable U V light on the sloping inlet grille It should be positioned centrally along the length of the grille The power lead ca
80. tions followed by Direct Control Note that as a default this option is selected Whenever the controller and computer are Dis Connected the fan will return and control to the setpoint speed see 8 9b Exhaust Fan Speed Manual adjustment of the exhaust fan speed is provided for in a similar way to that of the main fan However setting of the exhaust fan when fitted constitutes an important part of the cabinet setting up process This task will follow calibration and is described in Section 12 2 Exhaust Fan Setup 10 PC Clock This field shows the time and date of the PC s internal clock It is important that the PC s clock always reports the correct time and date as this is the reference used to re set the controller clock whenever a connection is made between the two devices the controller clock is automatically updated from the PC clock For security purposes the controller has been programmed so that it will not accept a time update grossly behind its current reference 10 5 MENU BAR Facilities available from the top menu refer to Fig 13 are described in the following paragraphs To display the possible options click with the pointer on the appropriate text The available options will then be displayed in black text Options not available will be shown in grey 10 5 1 File The Load and Save options allow sets of configurations to be stored on computer disc and retrieved for subsequent use This therefore will save
81. ts functions and controls This manual should be thoroughly studied before the cabinet is used When commencing use the following procedure must be followed for safe operation abbreviated instructions are shown on the Control Panel a Plug in the power lead if applicable Switch on power at the switch located at the power entry point Bioquell STD9000 002 Tele 44 0 1264 835800 Walworth Road Revision 2 Fax 44 0 1264 835801 Hampshire SP10 5AA Email enquiries bioquell com United Kingdom Page 12 of 64 Web www bioquell com b c d e f Confirm that the blue LED on the control panel is lit indicating that mains power is applied to the cabinet Press and hold down the TEST button All LEDs on the keypad should light and the buzzer should sound If not the cabinet should not be used and maintenance advice sought Open the window to operating position see section 5 2 and press the FAN button if prompted enter PIN number The fan will take about 1 minute to stabilize during this period the alarms are muted It is possible that after the stabilising process the buzzer sounds and the display advises of an airflow alarm The possible causes are as follows i The first time the cabinet is switched on following a maintenance operation involving use of the set up software ii The environment of the cabinet is at the extremes of its operating temperature 10 35 C

Bioquell ABS Class II Op Man (Revision 2)

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