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1. W3 65 0001 PLI 100 Pico Injector e 5 pressures inject balance clear fill and hold e Reliable e Femtoliter to microliter injections e Reproducible performance e Easy to use e Popular applications Injection of mouse frog zebrafish and other oocytes Extracellular brain injections Injection of DNA mRNA microbeads neurotransmitters kinases and other proteins e Most Published Injector The PLI 100 Pico Injector reliably delivers a wide range of volumes through micropipettes by applying a regulated pressure for a digitally set period of time Compressed gas allows the user to deliver desired volumes from femtoliters to microliters while simultaneously holding a cell Whether you need to do large injections into capillaries or very small injections into mammalian nuclei the PLI 100 is well suited for your experiment The PLI 100 has become a favorite of prestigious national microinjection workshops like Cold Spring Harbor Laboratories and other researchers worldwide Other companies have tried to design similar systems but the PLI 100 remains unparalleled in terms of ease of use durability precision and cost Researchers Say The PLI 100 is very robust in constant use in our lab Most importantly the PLI 100 valves prevent cytosol and yolk backflow into my pipette after oocyte injection Its very easy to control The PLI 100 is heavily used on a daily basis and works great
2. I like the fact that can use the PLI 100 with TTL pulses It works great in conjunction with my imaging system The balance pressure is a good option My injection pipettes rarely clog Easy to Use Inject with the touch of a button or a tap of the foot switch it s as easy as that Reproducible Pressure to the pipette is controlled precisely through a multi turn regulator and is reported digitally for easy repeat Injection time is digitally controlled in 10 msec steps between 0 01 to 0 99 seconds and in 1 second steps between 1 to 99 seconds Pipette tip diameter and hence delivered volume are easy determinations once the timing and pressure are known Versatile Deliver volumes from femtoliters to microliters with the same instrument resulting in a wide range of applications Pressure Capabilities The PLI 100 features two negative and three positive pressure pneumatic capabilities The negative or vacuum functions allow the user to 1 Fill micropipettes from their tips reducing wastage of valuable injectables 2 Provide a means to secure and manipulate a cell using a holding pipette Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com PLI 100 Medical Systems Pico Injector continued The positive pressures allow the user to 1 Eject precise amounts of fluids 2 Create a balance pressure which prevents backflow i
3. 1 Power Cord 1 1 User s Manual 1 1 Specifications Output Channels Input Gas Pressure Clearing Pressure Clear Hold Pressure Injection Pressure Balance Pressure Fill Vacuum Hold Vacuum Drain Vacuum Repeatability Display Accuracy Timer Setting Range Counter Setting Range Savable Sequences Programmable Steps Remote Control Ports Signal Output Ports Power Consumption Four PM 4 or eight PM 8 injection perfusion outputs one synchronized drain out and one cell hold channel 30 to 100 p s i Same as input pressure 0 to 3 p s i regulated 0 05 to 60 p s i regulated 0 05 to 10 p s i regulated 0 to 24 in Hg regulated 0 to 30 in of water regulated 0 to 30 in of water regulated 0 02 p s i 0 05 p s i for pressure 0 2 in for vacuum lt 2 in H 0 10 msec to 327 67 sec 10 msec resolution for whole range 1 to 255 16 sequences 32 steps for each sequence DB9 connector for remote key pad foot switch jack and BNC for negative TTL trigger jack connector for injection foot switch Eight BNCs for injection signal on output port 1 to 4 100 120 or 220 VAC 35 W Order W3 69 0152 W3 69 0153 Model PM 4 PM 8 Product 4 Channel Pressure Injection System 8 Channel Pressure Injection System Options and Accessories W3 69 0154 W3 69 0155 W3 69 0156 W3 69 0157 W3 69 0158 W3 69 0160 W3 69 0163 W3 69 0164 W3 69 0165 W3 69 0166 W3 69 0167 IP 1 OP 1 OP 2 F
4. 230V 50Hz W3 72 6197 Intralux 5100 Light Source JAP 100V 60Hz Accessories and Spares W3 72 0227 Halogen Spare Lamp EKE 21V 150W W3 72 0219 Blank Filter Holder for Intralux 5100 NCL 150 W3 72 0220 Blue Filter for Intralux 5100 NCL 150 W3 72 0221 Red Filter for Intralux 5100 NCL 150 W3 72 0222 Green Filter for Intralux 5100 NCL 150 W3 72 0223 Yellow Filter for Intralux 5100 NCL 150 W3 72 0224 Daylight Filter for Intralux 5100 NCL 150 W3 72 0310 Set of Filters Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com V Lux 1000 and NCL 150 Fiber Optic Cold Light Sources e For Microscope Illumination e Constant color temperature and light emitting angle e Continuous intensity adjustment e Low cost alternative to the Intralux series e Use with fiber optic ringlights goosenecks and flexible light guides V Lux 1000 Cold Light Source The V Lux 1000 is a low cost quality fiber optic cold light source featuring many high end features such as compact design constant color temperature long lamp life and low noise vibration It is ideal for any microscopy applications The V Lux 1000 will provide reliable dependable high intensity cold halogen light for use with all Volpi fiber optic components in microscopy applications Although it is factory equipped with DDL Lamps EKE bulbs may be easily substituted for slightly more light output Has integrated
5. 776 1278 www warneronline com
6. Fitting Connects with PLI IHO N Gas Input Hose Tygon Tubing 9 ft x 0 250 in 6 35 mm x 0 125 in 3 18 mm x 0 062 in 1 57 mm Lx OD x ID x Wall Thickness Connects to PLI IHA and PLI 90 100 Gas Input Brass Screw Fitting Type Old Gas Input Hose Tygon Tubing 9 ft L x 0 250 in 6 35 mm OD x 0 125 in 3 18 mm ID x 0 062 in 1 57 mm Wall Thickness Connects to PLI IHA and PLI 90 100 Gas Input Quick Connect Disconnect Type New Output Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders Brass Screw Fitting Type Old Output Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 90 100 Gas Output Quick Connect Disconnect Type New Holding Hose with Cell Release Bulb 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Brass Screw Fitting Type Old Holding Hose with Cell Release Bulb 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Quick Connect Disconnect Type New Order Model Product Pipette Holder Adapter Parts W3 65 0013 W3 65 0014 W3 65 0017 W3 65 0015 W3 65 0018 W3 65 0019 W3 65 0020 W3 65
7. Tools S10 UII I O seh 2 342 IntraLED 2020 and Filters Fiber Optic Cold Light Sources IntraLED 2020 This high power LED light source represents a viable alternative to halogen light sources It achieves its high light yield of more than 6 MLux at a total power consumption of just 15W With a color temperature of 6500K the IntraLED 2020 emits a very white light that is far superior to halogen lamps The illumination intensity is controlled manually using keys on the front panel or digitally via an RS 232 interface The IntraLED 2020 is suitable for clean room applications and outperforms halogen light sources with respect to bulb life and efficiency Specifications Optical Characteristics Light Source Illumination Intensity Efficiency Max Optical Efficiency Aperture Viewing Angle a 2 Color Temperature Typical Color Reproduction Index Degradation Period of LEDs Optical Output Regulation of emittance on Vs area illuminated1 LED Radiation Class Electrical Characteristics Supply Voltage Vs Current Consumption Communication Connector Mechanical Characteristics Dimension Enclosure Forced Cooling Fan Noise Enclosure Rating Adapter Weight Ambient Conditions Ambient Temperature Humidity Order Product W3 72 0322 IntraLED 2020 W3 72 4667 High Power LED White CIE 1931 gt 6 000 000 Lux gt 440 000 Lux W 65 m W 10 6500K 80 gt 50 000h1
8. lt 10mm 2 5 inch Constant Vs 12V 10 and T 10 C to 40 C 1M LED Product 12V DC Approximately 1 250mA RS 232 M8 Male 4 Poles2 89mm x 84mm x 159mm including connectors Aluminum body anodized 39dB A IP 20 15mm standard Volpi ferrule Approximately 1 1kg Operation 0 C to 40 C Storage 0 C to 50 C Maximum 95 relative not condensing IntraLED 2020 with Schott Foster Receptacle e Blue Filters enhance red and yellow objects and lighten blue and green objects e Red Filters will darken blue and green objects and are useful for black and white applications e Green Filters increase contrast for blue and red objects and brighten yellow and green objects e Yellow Filters of specific band widths may be used as a safe light when working with photosensitive materials e Daylight Conversion Filters increase the Kelvin Temperature of the halogen light to 5500 K which ts daylight Daylight Filters reduce glare from highly reflective material such as muscle tissue and metals Light Source Filters Light Source Filters provide contrast enhancement When the composition of an object is made of two or more colors a filter may be used to darken the color that is most important and therefore be able to observe it better When the composition of an object is pale a filter of the opposite or complementary color may enhance the detail Specifically when the composition of the object is highly dense and
9. 0021 W3 65 0022 PLI PH1 PLI PH1A PLI SRG PLI SRG1 5 PLI SSB PLI PC PLI HN PLI PH KIT PLI PHA Stainless Steel Pipette Holder 130 mm L for 1 to 1 5 mm OD Glass Pipettes Stainless Steel Pipette Holder 80 mm L for 1 to 1 5 mm OD Glass Pipettes Silicone Rubber Gasket Replacement for use with PLI PH1 and PLI PH1A 10 2 cm 12 in Silicone Rubber Gasket Replacement for use with 1 5 mm Glass Pipettes Stainless Steel Bushing Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Stainless Steel Pipette Cap Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Stainless Steel Hose Nut Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Pipette Holder Hardware Replacement Kit for use with PLI PH1 and PLI PH1A Pipette Holders Includes 1 each PLI SSB PLI PC PLI HN and PLI SRG Stainless Steel Pipette Holder PLI PH series Adapter for Eppendorf ECET FEMTOTIP Optional Easy Insert Acrylic Pipette Holder Capillary Glass OD W3 64 1626 W3 64 1627 W3 64 1628 W3 64 1629 A016 1 0 A016 1 2 PLI PPH A016 1 2 Tubing Diameter 2 mm 0 08 in Acrylic Pipette Holder for 1 0 mm Pipettes Acrylic Pipette Holder for 1 2 mm Pipettes Acrylic Pipette Holder for 1 5 mm Pipettes Acrylic Pipette Holder for 2 mm Pipettes Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 e Fax 203 776 1278 www warneronline com mic
10. Fax 203 776 1278 www warneronline com 335 Vibratome 3000 Vibratome 1000 Plus No No No 15mm Yes 5 um 60 x 40 x 15 mm 0 5 mm s adjustable 5 0 mm s max 40 mm Yes Optional Optional 5 um Yes Yes Yes Yes Yes 1 5mm Optional No Yes Optional No No No No W3 69 0350 W3 69 0351 Vibratome 1500 The Vibratome 1500 is the newest member of the Vibratome family and features a fully automatic tissue sectioning system Users can simply enter the number of sections and thickness required and the Vibratome 1500 does the rest Through the use of an easy to read LED display and simple controls the Vibratome 1500 makes serial sectioning an easy task Vibratome 1500 No No No 15 mm Yes 5 um 60 x 40 x 15 mm 0 5 mm s adjustable 5 0 mm s max 40 mm Yes Optional Optional 5 um Yes Yes Yes Yes Yes 1 5mm Optional Yes Yes Optional No No No No W3 69 0446 W3 69 0447 Vibratome 3000 Plus Yes Yes Yes 15 mm Yes 5 um 60 x 40 x 15 mm 0 5 mm s adjustable 5 0 mm s max 40 mm Yes Standard Standard 1 um Yes Yes Yes Yes Yes 2 0mm Standard Yes Yes Optional Yes Yes Yes Yes W3 69 0442 W3 69 0443 All Vibratomes are supplied complete with Blade Angle Indicator Vice Type Specimen Holder Specimen Mounting Blocks Bath 146 x 146 x 50 mm 5 75 x 5 75 x 2 in Black Buffer Bath Dust Cover Magnifyi
11. V Block Adapter Adapter for holding special specimen mounts in the Vibratome vice black anodized Order Product W3 69 0371 V Block Adapter Section Mounting Device A simple device designed to mount floated histological sections onto standard glass slides This product facilitates the mounting of all types of thin 2 um to 100 um tissue slices It is especially useful for mounting tissue slices not supported by embedding material such as those produced with a Vibratome freezing microtome cryostat or sliding microtome It also simplifies the mounting of tissue sections embedded in a water miscible supporting medium such as polyethylene glycol PEG Order Product W3 69 0381 Section Mounting Device Specimen Tray This tray holds the tissue in an appropriate buffer Replaces the standard mounting block Surrounded by ice in the bath it will keep the buffer cold without disturbing the sections The tray saves buffer allows easier clean up and keeps contamination to a minimum A rectangular projection on the bottom of the tray fits directly into the specimen vice Inside dimensions 76 x 55 x 9 5 mm 3 x 2 1875 x 9 375 in Order Product W3 69 0369 Specimen Tray Deluxe Specimen Bath The Deluxe Specimen Bath mounts inside the existing black Vibratome bath This large removable bath allows for the separation of buffer and ice kept in the original black bath The Bath can be sterilized and has round mounting disks that can
12. cycle mode after selecting single or recycle operation the start push button will initiate the ejection pumping action In this mode each succeeding pump module is automatically triggered after the pause time of the preceding event has been completed Both eject and pause times can be preset to cover a range from 30 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second resolution Trigger Mode When the TRIG switch is energized the eject time interval will be started by virtue of an externally applied trigger pulse fed to the respective inputs on the CM I control panel Eject timing intervals can be preset to cover a range from 30 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second resolution Gate Mode When the GATE switch is energized pumping action will be started by virtue of a gate input signal applied to the respective trigger gate terminals on the CM I control panel The eject and pause time settings are Fully pulled Micron tip Pre pulled Blank e Easy filling e Minimal tip resistance e Minimal leakage e Available pre pulled or fully pulled not operative in this mode since the eject time function is slaved to the duration of the gate Input Continuous Mode Eject pump action is continuously maintained when CONT switch is energized Termination Mode When the TERM switch is energized the output pressure is automatically diverted from the prepa
13. resettable fuse transformer This unit has a fixed power cord Specifications Uniformly Illuminated Light Guide Diameter 13 mm Rated Lamp Life DDL 800 hrs EKE 350 hrs DDL 3120 K EKE 3190 K Crescent shaped diaphragm Constant Color Temperature Light Adjustment Lamp DDL 20V 150W or EKE 21V 150W Dimensions H x W x D 9 5 x 17 1 x 21 0 cm 3 75 x 6 75 x 8 25 in Weight 4 5 kg 9 92 Ib Average of continuous operation Manufactured in U S A Order Product W3 72 0215 V Lux 1000 120 V USA Canada W3 72 0216 V Lux 1000 230 V W3 72 0217 V Lux 1000 230 V W3 72 0218 V Lux 1000 230 V W3 72 0231 V Lux 1000 230 V W3 72 0232 V Lux 1000 230 V Accessories and Spares W3 72 0227 Halogen Spare Lamp EKE 21V 150W W3 72 0219 Blank Filter Holder for Intralux 5100 NCL 150 W3 72 0220 Blue Filter for Intralux 5100 NCL 150 W3 72 0221 Red Filter for Intralux 5100 NCL 150 W3 72 0222 Green Filter for Intralux 5100 NCL 150 W3 72 0223 Yellow Filter for Intralux 5100 NCL 150 W3 72 0224 Daylight Filter for Intralux 5100 NCL 150 W3 72 0310 Set of Filters Argentina Austria Belgium Brazil China Denmark Finland France Germany Indonesia Netherlands Norway Sweden Switzerland Thailand Vietnam a Australia New Zealand Fiji South Africa Ireland ltaly Warner Instruments Phone 203 776 0664 e Toll Free U S 800 599 4203 e Fax 203 776 1278 www warneronline com e 20V 150W e Constant color t
14. tips Automatic current neutralization for minimal electrical artifacts Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 325 uo dafulo1IW O eh 2 326 Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection continued BH 2 Mainframe Chassis The BH 2 Mainframe Chassis is pre wired to accept one Control and Balance module and five lontophoretic Pump Modules and or Pneumatic Pump Modules The selected modules are specified by the researcher and depend on applications Although all components are ordered separately the unit is assembled calibrated and balanced by Harvard Apparatus before shipping BM 2 Balance Module Balance and More In addition to providing current neutralization automatic feedback and control of inverse sum of all pump currents the balance module has independent capability of current pump settings with a working range of 0 to 500 nanoamperes The Balance Module includes a digital display time clock provisions for electrical and manual cycle start stop and single cycle recycle switch trigger and gate input terminals to initiate externally controlled eject pumping action of respective modules Analog input for balance or drive with override capability Analog output for monitoring of unbalance currents Brain Slice Chambers are available in a variety of formats Please visit our website for more
15. 5x Auxiliary Lens for Zoom Stereo Microscopes Adapter for Fiber Optics Ring Light or Fluorescent Ring Light 15W Halogen Lamp and Reflector 5W Fluorescent Boom Stand for Stereo Microscope Ringlight Adapter 66 mm OD for use with Ringlights see pages 343 Microscope for Microforge De Fonbrune includes W3 99 0442 adapter mounting rod Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 900 Series Inverted Microscope W3 69 3100 Inverted Microscope with Phase Contrast Objectives and Video Photo Port Substage e Ideal for Tissue Culture and Cell Biology e Long working distance Plan objectives 4x 10x 25x and 40x e Long working distance Plan phase objectives 10x 25x and 40x e Optional SLR 35 mm photographic system e Optional CCTV system e Large mechanical stage with low position coaxial x y movement e Wide selection of filters e Swing out condenser to accommodate extra large objects Ergonomically designed and precisely manufactured yet simple to use the Harvard Apparatus SZM 180 Stereo Zoom Microscope provides versatility and high quality images for brightfield and phase contrast observation With standard built in photographic and video port the HAI 900 Is the ideal instrument for tissue culture and other advanced applications Microscope is supplied complete with the following components stand and observation head WF10x eyep
16. 8 10 13 Bushing Diameter 5 10 10 13 16 Single Arm Lengths 50 cm W3 72 0283 W3 72 0287 W3 72 0291 W3 72 0295 W3 72 0299 20 in 100 cm W3 72 0284 W3 72 0288 W3 72 0292 W3 72 0296 W3 72 0300 40 in 150 cm W3 72 0285 W3 72 0289 W3 72 0293 W3 72 0297 W3 72 0301 60 in 200 cm W3 72 0286 W3 72 0290 W3 72 0294 W3 72 0298 W3 72 0302 80 in Does not fit standard focusing lense Twin Arm Lengths 100 cm W3 72 0313 W3 72 0316 40 in 150 cm W3 72 0314 W3 72 0317 60 in 200 cm W3 72 0315 W3 72 0318 80 in Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com illuminators Tools gt D 2 jaa 349 SJO EUIWIN I a 2 2 350 Light Guide Accessories Made in the USA e Fixed point illumination e Accurate adjustment for quick simple and precise positioning e Unrestricted movement 220 mm radius of action e Very durable Articulating Arm with Heavy Steel Base Articulating Support Arm provides unrestricted movement and accurate fine adjustment for fixed point illumination Adjustment is simple and precise with 220 mm radius of action Articulating Arms are available with M6 M6 and M6 MB thread ends Flexible Light Guides can be attached with small 9 to 12 mm OD and large 11 to 16 mm OD holders depending on flexible light guide outer ferrule diameters 3 Compone
17. 99 sec in 1 sec steps Digital three and a half digits Internally timed or externally triggered Foot or panel switch Inject balance clear output port 100 110 220 240 VAC 220 W Optional inject and gating Input hose output hose and power cord 6 8 kg 15 Ib 11 x 38 x 25 5 x cm 5 x 15 x 10 in Product W3 65 0004 PLI 90 BASIC Pico Injector with Injection W3 65 0005 PLI 90 PLUS Balance and Clear Pressures Includes Input Hose PLI IHN and Output Hose PLI OHN Handle Power Cord and Instruction Manual Applications Basic setups that do not need suction Pico Injector Basic Model and Acces sories Shown Above Plus One Each PLI FS Foot Switch PLI PH1 Pipette Holder and PLI IHA Input Hose Adapter Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com PLI 100 and PLI 90 PLI 100 and PLI 90 Accessories Order Accessories W3 65 0029 W3 65 0030 Hosing W3 65 0006 W3 65 0007 W3 65 0008 W3 65 0009 W3 65 0010 W3 65 0011 W3 65 0012 Model PLI FS PLI RM PLI IHA PLI IHO PLI IHN PLI OHO PLI OHN PLI HHO PLI HHN Product Footswitch for Inject Hold Fill or Gate Order more than one if frequent use of footswitch is needed for more than one of these functions Frame to Mount PLI 90 or PLI 100 in 19 in Wide Instrument Rack Input Hose Adapter in Male W3T Pipe Thread Gas Bottle Regulator
18. Micropipettes Micropipette 7 Barrel 1 5 mm OD Borosilicate Glass Pre Pulled Plank 20 per box Micropipette 7 Barrel 1 5 mm OD Borosilicate Glass Fully Pulled Approx 20 um Tip 10 per box W3 65 0208 MS 7MT Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 329 uo dafulo1IW a D 2 lt 330 Nanoject Il Auto Nanoliter Injector Micromanipulator and Stand sold separately NANOUECT A E e Lower volume 2 3 nl capability e Positive displacement technology e 2 3 to 69 0 nl range of volumes e Minimal vibration e New improved high torque motor e Membrane key pad e Improved micropipette holder e Two speed injection fill rates e Auto Home capability The Nanoject Il is the latest development in the Nanoject family of microinjectors One unique features of the Nanoject Il is the use of a high torque motor to provide smooth operation resulting in substantially reduced tip movement A new collect configuration holds the micropipette more securely thereby reducing air infiltration and oil leakage The two speed fill inject mode allows the user to fill and inject at half speed facilitating the use of smaller micropipettes The handling of more viscous samples is also improved All of the above factors enhance the precise delivery of sample Volumes for the Nanoject II range from 2 3 nl to 69 nl The smaller tips and smaller
19. SW PM KP RK 2 PP 7 PP 4 HOLD 1 HOLD 4 HOLD 7 Input Tubing with Connector Output Tubing with Connector and 4 5 ml Vial Output Tubing with Connector only Footswitch Remote Key Pad Rack Mounting Kit with Handles for PM 4 8 Pulled Seven Barrel Pipette each Pulled Four Barrel Pipette each Holder for Single Barrel Pipette Holder for Four Barrel Pipette Holder for Seven Barrel Pipette W3 69 0156 Output Tubing with Connector for use with W3 69 0152 see above W3 69 0153 see above W3 69 0150 see page 245 W3 69 0151 see page 245 W3 69 0154 Input Tubing with Connector for use with W3 69 0152 see above W3 69 0153 see above W3 69 0150 see page 245 W3 69 0151 see page 245 W3 69 0164 and W3 69 0163 Pulled Pipettes for use with W3 69 0152 see above W3 69 0153 see above microinjection W3 69 0155 Output Tubing with Connector and 4 5 ml Vial for use with W3 69 0152 see above W3 69 0153 see above W3 69 0150 see page 245 W3 69 0151 see page 245 gt D jaa Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 e Fax 203 776 1278 www warneronline com 333 Huljdwes anssiy T 2 a lt 334 Vibratome Tissue Sectioning Systems W3 69 0353 Vibratome 1000 Classic The Vibratome series of fresh tissue sectioning systems allows sectioning of fresh or fixed tissue without freezing or embedding The patented vibrati
20. Section Page No Microinjection Introduction to Microinjection 318 Pico Injection Micromanipulation System PLS 1 319 Pico Injector Systems PLI 100 320 321 Pico Injector Systems PLI 90 322 PLI 100 amp PLI 90 Injector Accessories 323 Air 1 Ultra Low Noise Air Compressor 324 BH 2 Neuro Phore System 325 329 Nanoject Il Auto Nanoliter Injector 330 Screw Actuated Air Syringe 331 Multi Channel Pressure Injection System PM 8 amp PM 4 332 333 Tissue Sampling Vibratome Tissue Sectioning System 334 336 Mcllwain Mechical Tissue Chopper 337 Microscopes Stereo Zoom Microscopes SZM 180 338 Inverted Microscope 900 Series 339 Illuminators Intralux 5100 Fiber Optic Cold Halogen Light Sources 340 V Lux 1000 Fiber Optic Cold Light Source 341 NCL 150 Fiber Optic Cold Light Source 341 IntraLED 2020 Fiber Optic Cold Light Source 342 Light Source Filters 342 Spare Lamps and Power Cords 343 Ring Light Adapters 343 Fiber Optic Ringlights 344 347 Gooseneck Light Guides 348 349 Articulating Arm and Modular Accessory System 350 Glass Light Fiber Backlights 351 Brightfield Darkfield Base 351 Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com PLI 100 see page 320 Vibratome 1000 Classic see page 334 SZM 180 see page 338 Intralux 5100 see page 340 Cell Biology Tools 317 Introduction to Microinjection The use of Glass Needle Fine Glass M
21. Storage Block Specimen Retrievers and Section Mounting Device W3 98 2528 Feather Blades Listed below and to the right are optional accessories to the Vibratome Tissue Sectioning System Select your options based on your tissue slicing requirements Sapphire Knife This sapphire microtomy knife makes it possible for the first time to cut thin Vibratome sections with microscopic resolution similar to that obtained with thick 1 um plastic sections The sapphire knife allows routine serial sectioning with a Vibratome 1000 Plus of a thickness in the 6 to 10 um range revealing sub cellular features never before seen With a Vibratome 3000 sections of mildly fixed tissue as thin as 2 um may be obtained The sapphire knife can be re sharpened Order Product W3 69 0378 Sapphire Knife Specimen Mounting Blocks These Specimen Blocks are utilized for mounting specimens and placing in the Vibratome holding vice The blocks are annodized black for easy specimen viewing and can be reused Order Product W3 69 0370 Specimen Mounting Block Specimen Mounts 12 inch Head Specimen mounts are designed to allow for the rotation of the specimen in the Vibratome vice assembly for sectioning at different horizontal orientations Order Product W3 69 0372 Specimen Mount 12 inch Head Specimen Retriever This is a perfect tool for retrieving free floating sections from the bath Order Product W3 69 0380 Specimen Retrievers pkg of 5
22. W3 72 4662 Ring Polarizer Set with Glass Analyzer for all LED Ringlights Slim Line Power Supply works with all LED Ringlights W3 72 0321 e Twice as bright e Available with white or yellow LEDs e Applications Scratch amp Flaw General purpose illumination Inspection yellow LED Twin Row Spot Focused LED Ringlight Uniform shadow free illumination LED light source with extremely stable and homogeneous light output Optimal working distance 3 6 inches Includes on off switch and power supply Specifications Illumination Type White 7800K High Intensity LED Yellow 6400K Expected Life gt 10 000 Hours Slim Line Power Supply 110V included Maximum Voltage 24 Volt DC Cable 1 5M Wiring Black GND White Black Anodized Aluminum Body Average of continuous operation Order W3 72 0319 W3 72 0320 W3 72 0321 Product Twin Row Spot Twin Row Spot Slim Line Power Focused LED Focused LED Supply included Ringlight White Ringlight Yellow Number 84 84 of LEDs Power 7 2W 7 2W Consumption Mounting 66 mm 66 mm Diameter Working 75 150 mm 75 150 mm Distance 3 6 inches 3 6 inches Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Fiber Optic Ringlights continued Standard Ringlight Full circular glass fiber ringlight provides homogeneous shadow free illumination for working distances of 25 to 90 mm Inside diameter
23. al 0 through 9999 Internally timed or externally gated Front panel foot switch or external TTL pulse BNC p s i kPa switch selectable BNC connector 10 mV p s i Inject balance clear output port 100 110 220 240 VAC 220 W 0 1 full scale Inject fill hold and gated provided in plus and deluxe pkgs 6 8 kg 15 Ib 11 x 38 x 25 5 cm 5 x 15 x 10 in Input output and holding hoses Product PLI 100 Basic Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord and Instruction Manual PLI 100 Plus Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Foot Switch W3 65 0029 Pipette Holder W3 65 0013 and Input Hose Adapter PLI 100 Deluxe Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Two Foot Switches Two Pipette Holders and Input Hose Adapter microinjection gt D jaa D 321 uolNdafulo1IW a D 2 lt 322 PLI 90 Medical Systems Pico Injector Precise and reproducible injection W3 65 0004 PLI 90 Pico Injector e 3 pressures inject balance and clearing e Eliminates backflow into pipette after injection e Reproducibly delivers femtoliters to micr
24. al devices such as a computer event counter etc Specifications Current Pump Compliance 105 V linear constant current source Pulsing controlled by Ejection Timing Mode Switch amplitude adjustable by 10 turn ejection control and range switch from 0 to 50 or 0 to 500 nA polarity is selected by polarity switch accuracy of I nA Ejection Current Red LED lamp indicates Eject time period green LED lamp indicates pause time period Ejection Indicator Amplitude adjustable by front panel dial from 0 to 50 nA polarity automatically set opposite to ejection current polarity socket x3 Retention Current Lemo miniature receptacle ground referenced 5 mV nA input impedance 100 kO socket x3 Analog Input Lemo miniature receptacle ground referenced 5 mV nA socket x3 Lemo miniature receptacle TTL pulse time incident with eject pulse Analog Output Sync Output Current and Resistance Digital Meter Display 3 digits and sign Metering System Resistance Current Switch Current Mode Switch in center nA position digital display reads total current in nA passed through micro electrode pipette sum of retention and ejection current Switch in either pos or neg MQ position digital display reads actual electrode barrel resistance in MO derived by passing positive or negative constant current 50 nA through electrode pipette Resistance Mode Digital display will flash whenever electrode barrel resist
25. ance Compliance Exceeded exceeds working range of current pump i e when electrode resistance times current exceeds compliance of 105 V Indicator Voltage Readout Switch Depressing switch will cause digital display to read voltage across pipette Order Product W3 65 0603 IP 2 lontophoresis Pump Module Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection continued PPM 2 Pneumatic Pump Module Designed specifically for pressure injection of drugs in pharmacological studies of drug evoked responses i e synaptic discharges contraction etc Emphasis has been given to pressure control and regulation 0 to 30 p s i optional O to 10 or 0 to 90 p s i as well as precise timing The PPM 2 Module is comprised of a precise pressure regulator digital display transducer and a timing mode switch It connects to an external pressure source such as a compressed bottle of N2 which can be set to provide continuous or periodic pressure pulses ranging from 0 to 30 p s i Outputs include 0 to 1 volt proportional to output pressure as well as a sync pulse coincident with pressure cycle Operating Modes Ejection Timing and Mode Switch The mode switch provides five push button controls which include operations such as cycle trigger gate continuous and termination Cycle Mode In the
26. anner W3 69 1013 Blade Holder W3 69 1011 Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com tissue sampling gt D i co D 337 sadoosoJ9IW SUE a 2 2 lt 338 SZM 180 Stereo Zoom Microscope W3 69 3101 Trinocular Zoom Stereo Microscope With Illumination Stand and Video Adapter e High performance at an economical price e Superior optical design with outstanding resolution and brilliantly sharp images e Rugged all metal construction e Excellent zoom range from 0 7x to 4 5x e The parfocal zoom system keeps the image in constant focus while you zoom e Zoom control knobs are conveniently side mounted for smooth comfortable operation e Trinocular body with comfortable 45 inclination angle for fatigue free viewing e Trinocular body may be rotated 360 for group sessions e Dual diopter adjustment of 5 diopters enables you and your colleague to precisely set focus according to individual needs e Interpupillary adjustment range from 55 to 75 mm e Total magnification range extends from 3 5x to 90x e Widefield eyepieces of 10x 25x Harvard Apparatus SZM 180 Stereo Zoom Microscope provides outstanding quality at an affordable price It features high resolution Optics and precise optical alignment that assures crisp sharp and flat 3 dimensional images Select from two different mounts The standard
27. be easily removed for mounting specimens The disks and bath surface are marked with degrees of rotation for the repeatable placement and measurement of specimens Order Product W3 69 0440 Deluxe Specimen Bath Glass Knife Adapter The glass knife adapter allows you to routinely cut 20 um sections of fresh tissue and even thinner sections of fixed tissue Simply replace the standard razor blade holder with our special glass knife adapter and use ordinary 1 in wide glass knives The entire assembly is black anodized aluminum Order Product W3 69 0379 Glass Knife Adapter Rotating Stage Assembly This assembly allows a specimen to be oriented to the blade after it has been affixed to the specimen mount At a 20 blade angle the rotating stage assembly will adjust up to 15 still allowing 10 mm clearance Replaces the standard Vibratome vice assembly Everything is black anodized aluminum Order Product W3 69 0373 Rotating Stage Assembly Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Vicllwain Mechanical Tissue Chopper Slice Thickness F Speed Adjustment s Control PVC Disk Rheosiat Cutting X Table e ir T i i FJ 7 To Raise ChoppingArm Force On Blade Control Carriage Release e Less disturbance of cell structure than homogenizers or blenders e Slices up to 1 mm thick and cubes or prisms up to 1mm cross secti
28. c unit is supplied with an input hose output hose holding hose power cord and instruction manual The plus unit also includes a foot switch pipette holder and input hose adapter The deluxe unit includes all the previously mentioned accessories plus an additional foot switch and one pipette holder Accessories Available accessories for the PLI 100 Injection Systems are located on page 320 Warner Instruments Phone 203 776 0664 e Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Specifications Input Gas Pressure Injection Pressu re Balance Pressure Fill Vacuum Holding Vacuum Clearing Pressure Injection Timer Pulse Width Injection Count Display Duration Mode Time Trigger Pressure Units Pressure Monitor Pressure Reado Line Voltage Power Usage ut Meter Accuracy Foot Switches Weight Dimensions H x WxD Accessories Supplied Order W3 65 0001 W3 65 0002 W3 65 0003 Model PLI 100 PLI 100 Plus PLI 100 Deluxe 70 to 105 p s i 480 to 720 kPa 0 2 to 60 p s i 413 kPa regulated multi turn control 0 1 to 3 5 p s i 68 9 kPa regulated multi turn control other ranges available upon request Internally produced 12 0 p s i 82 kPa unregulated Internally produced 0 to 3 in H 0 0 to 0 75 kPa or 0 to 0 1 p s i regulated Input gas pressure unregulated 0 01 to 0 99 sec in 10 msec steps 1 to 99 sec in 1 sec steps Digit
29. cal sectioning components with enhanced specifications and features such as smaller section thickness increments increased sectioning speed faster return stroke speed increased maximum sectioning range drain function standard dual bath and finer height adjustment A stroke pause switch and adjustable light and magnifier are also part of the Vibratome 1000 Plus system The Vibratome 1000 Plus is a solid addition to the Vibratome family of products Vibratome 3000 Plus The Vibratome 3000 Plus sectioning system provides a versatile means of sectioning fresh or fixed animal or plant tissues This system employs a vibrating blade principle which allows sectioning without freezing or embedding The creation of artifacts the alteration of morphology the destruction of enzyme activities and other deleterious effect inherent in freezing or embedding procedures are thus avoided The Vibratome 3000 Plus incorporates a full english text display for added ease and usability The 3000 Plus utilizes the Vibratome Companies ZERO Z Technology which dramatically reduced z axis vibrations This system also has a dynamic stability system and deluxe bath system as standard The Vibratome 3000 Plus provides the best quality section in the Vibratome product line Vibratome 3000 Deluxe The Vibratome 3000 Deluxe includes all the enhanced features of the 3000 Plus sectioning system plus a built in thermoelectric cooling device for the
30. color saturated a filter of the same color will most likely enhance the detail on the object Custom filters of a specific band width are also available upon request Product Blank Filter Holder for Intralux 5100 NCL 150 Blue Filter for Intralux 5100 NCL 150 Red Filter for Intralux 5100 NCL 150 Green Filter for Intralux 5100 NCL 150 Yellow Filter for Intralux 5100 NCL 150 Daylight Filter for Intralux 5100 NCL 150 Set of Filters Order W3 72 0219 W3 72 0220 W3 72 0221 W3 72 0222 W3 72 0223 W3 72 0224 W3 72 0310 Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Light Source Spare Parts Spare Lamps and Lamp Socket Made in the USA Spare Lamps and Power Cords Order W3 72 0227 W3 72 0226 W3 72 0356 W3 72 0348 W3 72 0349 W3 72 0350 W3 72 0351 W3 72 0352 W3 72 0353 W3 72 0354 Product EKE Spare Lamp for Intralux 5100 V Lux 1000 Intralux DC 1100 21 V 150 W DDL Spare Lamp for Intralux 5100 V Lux 1000 20 V 150 W Spare Lamp Socket Round for Intralux 5100 V Lux 1000 Intralux DC 1100 NCL 150 Power Cord United Kingdom Power Cord France Power Cord Italy Power Cord Switzerland Power Cord Germany Power Cord The Netherlands Power Cord Spain Portugal Note All Volpi Light Sources are compatible with all Volpi Fiber Warner Instruments Phone 203 776 0664 Toll Free U S Optic Components and Accessorie
31. cular e 5 to 175 mm working distances e Shadow free e Direct mounting for 20 to 32 mm objectives Mini Ringlight Small full circular glass fiber ringlight provides homogeneous shadow free illumination for working distances of 5 to 25 mm Long Working Distance version is available for distances of 25 to 175 mm Can be used for fiber optic illumination on Compound Microscope objectives Mounting diameter ranges from 20 to 32 mm with the presence of 3 mounting screws without the need for adapters Standard Flexible length is 900 mm 40 in Longer lengths available Order Product W3 72 0246 Short Working Distance Mini Ringlight W3 72 0247 Long Working Distance Mini Ringlight e Large diameter e Full circular e 50 to 120 mm working distance e Diffuse light e High intensity SMD MAXI Ringlight Large full circular diameter glass fiber ringlight that provides homogenous shadow free illumination for working distances of 50 to 120 mm Large diameter and shallow fiber angle produces wonderful darkfield effect on large surface areas Inside diameter is 150 mm and can be adapted to SMD stations and stereomicroscope columns Standard Flexible length is 480 mm 19 in with longer lengths available on request Order Product W3 72 0245 SMD MAXI Ringlight Mini Ringlight Made in the USA with some components from Volpi AG SMD MAXI Ringlight Anti e S P m Eo CT Made in the USA with some components from V
32. dest light possible and the unique patented Crescent Shaped Diaphragm which provides constant color temperature and light emitting angle and continuous intensity adjustment Fiber optic components consist of e Ringlights e Goosenecks sliding Sleeve Volpi Patent e Flexible Light Guides e Backlights Specifications Average Lamp Life calculated value in continuous operation at line voltage 230V 121V 100V 110 Intensity 100 hours 100 Intensity 200 hours 90 Intensity 520 hours 80 Intensity 1700 hours 75 Intensity 3600 hours Color Temperature at 3433K 110 Intensity Illumination Intensity at 12 3 M Lux 110 Voltage at Lightguide Input DIN 58141 10 Maximum Illuminated 13 mm Light Guide Mechanical Light Guide 15mm Connector Light Adjustment electrical 8 step switch 75 to 110 Crescent shaped diaphragm Volpi Patent 16 steps EKE 21 V 150 W 230 V 50 Hz or 121 V 60 Hz or 100 V 60 Hz Halogen Reflector Lamp Line Voltages 10 Power Consumption 190 W Protection Class 1 Weight 4 6 kg 10 Ibs Housing Metal powder coated color RAL 9002 Approvals UL61010 1 CAN CSA 61010 1 CE Product Safety IEC EN 61010 1 2001 EMC Emission IEC CISPR 11 Class B Immunity IEC 61000 4 2 to 6 and 11 Manufactured in U S A Order Product W3 72 6195 Intralux 5100 Light Source USA 121V 60Hz W3 72 6196 Intralux 5100 Light Source EURO 230V 50Hz W3 72 6198 Intralux 5100 Light Source CH
33. ection Micromanipulator System The PLS 1 Pico Injection Micromanipulator System combines our popular PLI 100 pico injector a motorized micromanipulator and magnetic base in a single package The PLI 100 Pico Injector delivers a wide range of volumes through micropipettes by applying a regulated pressure for a set period of time The system features 5 pressures inject balance clear fill and hold The injector comes complete with input and output hoses foot switch and pipette holder For precise and convenient movement of the electrode our three axes motorized micromanipulator and push button controller are included This micromanipulator features hands free spatial resolution of 0 5 um which enables positioning in the sub micro range Flexible motor coupling ensures no vibration of probe during movement The MB B magnetic base provides a convenient position device for the micromanipulator S oss Order Model Product W3 64 1608 PLS 1 Pico Injector Micromanipulator System W3 65 0002 PLI 100Plus Pico Injector Pa W3 60 0571 Right handed motorized micromanipulator W3 60 0577 Push button controller W3 64 0060 MB B Magnetic base Warner Instruments Phone 203 776 0664 e Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 319 uo dafulolIW a 2 a lt 320 PLI 100 Medical Systems Pico Injector A full featured workhourse microinjector
34. ed Glass 90 mm 3 5 in Vial of 100 W3 69 0136 Standard O Ring W3 69 0137 Special Flared O Ring W3 69 0138 Replacement Wire Plunger W3 69 0139 Needle 30 ga x 51 mm 2 in Accessories Must be ordered separately W3 69 0140 Footswitch W3 69 0141 Support Base W3 69 0142 Universal Mounting Adapter see above photo Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Screw Actuated Syringes e For air assisted microinjection and microaspiration e Uncomplicated and easy to use e Accurate e Sensitive control e High suction pressure Screw Actuated Air Syringes These air assisted microinjection microaspiration syringes provide the user with sensitive control and an extremely low dead air volume The capacity of the syringe is 10 ml When required the syringes can be used to generate high pressure or suction The Screw Actuated Syringe is a small compact unit incorporated into a heavy circular base Its convenient dimensions enable it to be ergonomically positioned next to the control lever of a micromanipulator to facilitate single handed operation of both instruments The syringe is connected by hard polyethylene tubing to a micropipette which in turn may be fitted to a micromanipulator or positioning device suction injection is obtained by turning the metal colored actuator screw control on the top of the syringe The syringe provides a solution to t
35. emperature e Continuous intensity adjustment brightness e High Low setings e Filter shot e Use of DDL or EKE bulbs e Long lamp life e Low noise vibration NCL 150 Cold Light Source Specifications Rated Lamp Life Constant Color Temperature Light Adjustment High Low Settings Filter Slot Lamp Dimensions HxWxD Crescent shaped diaphragm 25 dB noise level 98 F Housing temperature DDL 20V 150W or EKE 21V 150W 10 5 x 21 9 x 21 9 cm 4 125 x 8 625 x 8 625 in Weight 4 5 kg 10 Ib Average of continuous operation Manufactured in U S A Order Product W3 72 0311 NCL 150 120V USA Canada W3 72 0312 NCL 150 230V Euro Accessories and Spares W3 72 0226 DDL Lamp 20V 150W W3 72 0227 Halogen Spare Lamp EKE Lamp 21V 150W W3 72 0220 W3 72 0221 W3 72 0222 W3 72 0223 W3 72 0224 W3 72 0238 W3 72 0262 W3 72 0264 W3 72 0267 W3 72 0266 W3 72 0329 W3 72 0269 W3 72 0273 W3 72 0342 W3 72 0287 Blue Filter Red Filter Green Filter Yellow Filter Daylight Filter Standard Compact Ringlight Ringpolarizer Set Diffuser Bifurcated Gooseneck 4 7 mm Bifurcated Gooseneck 5 4 mm Incident Gooseneck Focusing Lens Polarizing Cap for p n W3 72 0270 Backlight 2 x 2 Single Light Guide 10 mm All volpi light sources are compatible with all volpi fiber optics DDL 400 1400 hrs EKE 100 900 hrs DDL 3280 K 3140 K EKE 3310 K 3120 K illuminators gt D oe jaa D 341
36. es A safety limit switch is provided to prevent the machine overrunning and a quick return mechanism is fitted for the return of the table All parts coming into contact with the tissues are of stainless steel Finish is in white powder coat Note The above description is extracted from an article by H Mcllwain and H L Buddle in the Biochemical Journal Vol 53 No 3 pp 412 420 1953 by their permission Operation Positioning Blade Filter papers are placed on the circular table and a blade loosely clamped to the arm The arm is brought to rest on the stop rod by rotating the handwheel on the motor shaft and the blade pressed down with the fingers The blade is then securely clamped in this position The machine is switched on and it will probably cut the top filter paper This is removed and the machine is ready to take a specimen 5 2 Slice Thickness The thickness of slice is adjusted by means of the micrometer head The maximum thickness is 1 mm Each division on the thimble is 1 um Each small division on the barrel is 25 um 3 Force on Blade The force on the cutting arm can be adjusted by the front knob To increase tension turn in clockwise direction 4 Cutting Pull out the carriage release knob and slide the table to the left of the machine Place the specimen on the centre of the table and with the rheostat in the slow position switch on the bottom switch Increase the rheostat until the desired speed has been a
37. he problem of capillary action when working with small micropipettes A small balance equilibrating pressure can be maintained to offset the effects of capillary action A conveniently situated release button on top of the screw control is provided to achieve equilibration Order Product W3 69 0105 Screw Actuated Air Syringe e Ideal for oil filled injection aspiration e Easy to use e Hamilton syringe incorporated e Accurate e Sensitive control e Low dead air volume e High suction pressure e Easy to exchange or replace the syringe Screw Actuated Micrometer Driven Hamilton Syringe This screw actuated syringe is a micrometer actuated syringe mechanism mounted on a small base This syringe is for oil air assisted micro injections and micro aspirations Its compact dimensions enable it to be ergonomically positioned next to the control lever of a micromanipulator to facilitate single handed operation of both instruments microinjection Suction injection is achieved by using the rotary actuator with a fine micrometer thread which has a 15 mm movement range A 1 ml gastight Hamilton glass syringe with a Luer taper is incorporated A Luer taper hypodermic needle is mounted on the taper of the Hamilton syringe The syringe is connected by hard polyethylene tubing to a micropipette which in turn may be fitted to a micromanipulator or similar instrument The 1 ml Hamilton syringe can be easily exchanged for any other Hamilton 1700
38. hone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 339 sJo euIWN S 00 O zi ss i g lt 340 Intralux 5100 High Intensity Cold Halogen Light Source Recessed handle for portability Quick lamp exchange Crescent snap lock shaped diaphragm for brightness adjustment constant E color i temperature and light emitting angle Volpi Patent Low noise and 1 vibration free fan immun _ Superior light r intensity ee a Filter A insertion for 4 blue red green yellow and daylight filters 8 step switch for electrical q light Parimi No light eakage at the work station e Compatible with all Volpi Fiber Optic Components e Ergonomic design with small footprint e Quick bulb replacement reducing downtime e Solid metal housing designed for industrial use e Certified for electronic and mechanical safety e 150 watt cold light source with superior illumination intensity and long lamp life Intralux light sources have represented the best in Fiber Optic Cold Light Illumination the world for over 25 years They provide reliable dependable high intensity cold halogen light for use with all Volpi fiber optic components in microscopy applications The Intralux Series is equipped with the most advanced features and highest quality components available It includes InfraRed Reflecting Filters to ensure the col
39. ht Guide 5 4 mm x 500 mm fiber diameter each arm 20 long Ferrule diameter 10mm Gooseneck Accessories Standard Focusing Lens A spherical lens used to focus light to a concentrated spot Polarizing Cap Fits onto focusing lens Order Product W3 72 0269 Standard Focusing Lens W3 72 0304 Polarizing Cap for Standard Focusing Lens For Modular Accessory System see page 350 Fits on 10mm Ferrule Warner Instruments Phone 203 776 0664 Toll Free U S Made in the USA Focusing Lens with Polarizing Cap Made in the USA 800 599 4203 e Fax 203 776 1278 www warneronline com Gooseneck Light Guides continued Incident Gooseneck Light Guides k F Made in the USA e Bifurcated Gooseneck and flexible light guide combination e Attachable to columns of all brand microscopes through available adapters e Great flexibility in the placement of light source e Available in different lengths e Uses standard Gooseneck accessories Incident Gooseneck Light Guides This combination of a Semi Obedient Bifurcated Gooseneck and a Flexible Light Guide provides incident illumination Fiber Optic Incident Goosenecks attach to the columns of microscopes or other work station columns with specialized adapters Each arm can be positioned and adjusted independently The flexible length size can vary to allow the placement of the light source away from the microscope and work station Utilizes standard Goose
40. ht Guide 91 4 cm 36 in 91 4 cm 36 in 91 4 cm 36 in Met PVC Light Source Adapter Intralux Series Intralux Series Intralux Series Order Product W3 72 0342 Glass Fiber Backlight 5 x 5 cm 2 x 2 in W3 72 0343 Glass Fiber Backlight 7 6 x 7 6 cm 3 x 3 in W3 72 0344 Glass Fiber Backlight 12 7 x 12 7 cm 5 x 5 in p Bright Darkfield Base i e Transmitted bright darkfield illumination e Uses standard compact ringlight illumination e Low cost alternative e Compact design e Adapts to all brand name microscopes Brightfield Darkfield Base This is a low cost intense contrast rich transmitted light Bright Darkfield Base used with Standard or Compact Fiber Optic Ringlights It provides transmitted bright darkfield illumination Easily accessible knob allows quick and easy change between brightfield and darkfield Adapts to all brand name microscopes Order Product W3 72 0337 Bright Darkfield Base W3 72 0339 Polarizer to Base W3 72 0340 Diaphragm to Objective 0 5 0 63X W3 72 0341 Diaphragm to Objective 1 5 2 0X illuminators Note All Volpi Light Sources are compatible with all Volpi Fiber Optic Components gt D jaa D Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 351 cell biology tools Notes S OO ABojorg 129 Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203
41. i AG e Compact Metal Design e Flicker Free Light e 7 000 to 10 000 LED life e 7800 K Temp e Working Distance Range of 30 to 150 mm e Adaptable to all microscopes White LED Ringlights White Light Emitting Diodes LEDs are now available in a ringlight to produce uniform shadow free illumination comparable to that of fiber optic ringlights This exciting new technology is available in 5500 K Temperature Daylight and 7800 K Temperature for working distances of 30 150 mm Metal housing design produces minimal heat for cool light illumination Powered with an 8W 24V DC power supply included these ringlights offer flicker free illumination The most unique feature of these White LEDs is by far their rated life of 7 000 10 000 hours White LED ringlights have the standard 66 mm inside diameter and are adaptable to all microscopes and lenses through a series of available ringlight adapters This ringlight is supplied with slim line power supply Order Product W3 72 4656 Standard White LED Ringlight 30 70 mm Working Distance W3 72 4657 Diffuse Standard White LED Ringlight 30 90 mm Working Distance W3 72 4658 Spot Focused White LED Ringlight 70 160 mm Working Distance W3 72 4659 Daylight LED Ringlight 5500 K Temp Short Working Distance 30 70 mm Working Distance W3 72 4660 Daylight LED Ringlight 5500 K Temp Long Working Distance 70 160 mm Working Distance W3 72 4661 Rheostat Accessory for use with all LED Ringlights
42. icrocapillary Pipette based techniques for intracellular extracellular microinjection and perfusion has become a popular procedure in numerous areas of experimental biology research e g In vitro fertilization transgenics etc These techniques can best be described as micro surgical procedures that are conducted on a single cell using either a single or multiple barrel glass micropipette a precision positioning device micromanipulator and a microinjector or microperfusor The micropipettes used in these procedures are formed using a Pipette Puller The capillary glass tubing is heated to its softening point and pulled to create the proper size tip diameter and taper for the desired application The small tip diameters as low as 0 2 um of these micropipettes combined with the high precision of the Micromanipulator and Microinjection Perfusion Apparatus allows for precise and accurate delivery This precision allows for accurate and repeatable injections down to the sub picoliter liter range into or around various types and sizes of cells with accuracy to 0 1 micron The process of extruding substances through these micropipettes is accomplished through the use of either direct Harvard Apparatus Medical Systems Picoliter Injectors pages 319 to 323 hydrostatic pressure Pressure Injection or by moving charged ions that are the result of an applied electric field lontophoresis without the use of hydraulic flow Applications of mic
43. iece 11x centering telescope 4x Long working distance LWD plan objective and annulus 10x LWD phase contrast plan objective and annulus 25x LWD phase contrast plan objective and annulus and 40x LWD phase contrast plan objective and annulus Specifications Eyepieces Paired WF10X eyepieces Objectives Choice of 10x 25x and 40x Plan brightfield long working distance objectives Plan phase long working distance objective Built in video and photographic port lens and adapter required Video Capabilities Stage Large graduated mechanical stage with low position x y movement knob Condenser Centerable condenser with iris diaphragm and phase annulas ring slot swing out condenser holder with rack and pinion movement and safety stop Filters Filter disc with five positions Diaphragm Field Diaphragm and condensing lens Illumination Built in 6V 20W halogen illumination with intensity adjustment and lamp centering device 229 x 330 x 508 mm 9 x 13 x 20 in 16 5 kg 36 Ibs Shipping Dimensions Shippinig Weight Order Product W3 69 3100 900 Series Inverted Microscope with Phase Contrast Objectives and Video Photo Port W3 64 0302 Stage Adapter for HAI 900 Microscope permits use of Series 20 Imaging and Recording Chambers W3 69 3109 Replacement 6V 20 W Halogen Bulb for Inverted Microscope W3 69 3110 Replacement Fuse 250V 1 5A for Inverted Microscope microscopes gt D jaa D oO Warner Instruments P
44. ight perfusion vials one cell holding pipette and one drain pipette The four PM 4 or eight PM 8 injection perfusion output ports can be controlled separately or combined together to perform actions such as injection capillary action balancing suction or clear up The cell holding output port can produce an adjusted gentle suction to hold a cell eject a pressure to push a cell or completely clear the holding pipette Another drain output port can simultaneously produce an adjusted suction to drain and clear out liquid before the next channel micro perfusion The PM 4 8 can deliver different quantity agents and drugs from picoliters to continuous perfusion All output pressures and vacuums can be regulated and can be displayed real time on the front panel display Previous pressure readings can also be recovered on the display for setting comparison Besides programmable timers and counters there are 16 programmable and savable injection perfusion sequences with 32 programmable steps in each sequence Each sequence can be repeated continuously or triggered manually The interval time between steps can be programmed for automatic sequential cycle or manual trigger There are two foot switch connectors for sequential step trigger and injection trigger A digital remote port can be connected to an optional remote key pad or be controlled by a computer With exceptional versatility and extremely precise control either the PM 4 or 8 is an ideal m
45. information Order Product W3 65 0600 BH 2 Mainframe Chassis Does Not Include BM 2 Balance Module see Below W3 65 0602 BM 2 Control and Balance Module with OC 01 and NL 952 Application Note Working Unit One 1 each of BH 2 Main Frame MS 2 power supply BM 2 Control amp Balance module and any combination of one 1 to five 5 modules selected from IP 2 and or PPM 2 If less than five 5 DP 1 module is required to fill the spaces MS 2 Power Supply The AC power supply is self contained in a rack mounted cabinet and provides all voltages required to operate the Neuro Phore System The power supply interconnects with the mainframe via flexible cable The supply works with 115 or 220 VAC 50 60 Hz mains source Specifications Neutralization balance Pump Range Current Pump Digital Meter Display Unbalance Current Current Unbalance Current Mode Current Pump Mode Single Cycle Mode Recycle Mode Time Unit Switch Inputs Analog Input Analog Output Sync Output Output Connector Dimensions H x W x D Weight Specifications Outputs Dimensions H x W x D Weight Order W3 65 0601 Product MS 2 Power Supply with Power Cord Max 2500 nA automatically controlled Compliance 105 V linear constant current source manually adjustable 0 to 500 nA by pump control polarity selected OFF switch 3 digits and sign Pump Switch Digital display reads unbalance ground going curre
46. injection volumes of the Nanoject II allow users to perform injections into other specimens applications that previously could not be performed Positive displacement technology and the use of precision micropipettes eliminate the need for tedious calibration when the viscosity of the sample changes Contaminants in the sample do not hinder or change the injection volume as with some other injectors An optional universal mounting adapter provides easy mounting of the Nanoject II to a variety of micromanipulators and stereotaxic frames Needs Micromanipulator to attach to Support Stand Specifications Operation Automatic Glass Properties Softening point 780 C Glass Dimensions 1 14 x 0 053 mm 0 045 x 0 021 in OD x ID Injection Speed Fast 46 nl sec Slow 23 nl sec Fill Speed Fast 46 nl sec Slow 23 nl sec Empty Speed 92 nl sec Volume Range 2 3 nl to 69 0 nl Change Volume Dip switches 1 to 4 Change Inject Speed Dip switch 5 Weight 99 2 g 3 5 oz Order Product W3 69 0130 Nanoject II Nanoliter Injector 115 V 60 Hz US Plug W3 69 0131 Nanoject II Nanoliter Injector 230 V 50 Hz European Plug W3 69 0132 Nanoject II Nanoliter Injector 230 V 50 Hz UK Plug Replacement Parts W3 69 0133 Replacement Glass 88 9 x 1 14 x 0 053 mm 3 5 x 0 045 x 0 021 in L x OD x ID Vial of 100 W3 69 0134 Replacement Glass 177 8 x 1 14 x 0 053 mm 7 x 0 045 x 0 021 in L x OD x ID Vial of 100 W3 69 0135 Flar
47. ion of each of the pneumatic pump modules The self timing action permits each PPM 2 Pump Module to eject and pause for a predetermined period Actuating any of the self cycle control switches causes the respective PPM 2 Module to be engaged into the self timing mode The push buttons at the corresponding trigger gate terminals initiate the self cycle timing action Specifications Cycle Start Cycle Stop Push button and banana jack terminals Trigger Gate Inputs floating input optically coupled Input Voltage 5 to 15 V Minimum Trigger 5 usec ato V Pulse Width Sync Out Lemo miniature connector TTL pulse Single Cycle Mode In single cycle mode start switch or external trigger initiates each cycle Recycle Mode In recycle mode once start switch or and external trigger is actuated repetitive cycles will continue until stopped Time Unit Switch Two basic time units can be selected 10 msec or 1 sec In 10 msec position Eject and Pause time switches of PPM 2 Modules can be set to cover time range from 10 to 990 msec with 10 msec resolution In 1 sec position time scale is expanded to 1 to 99 sec with 1 sec resolution Power 115 VAC 50 60 Hz 100 or 220 VAC optional Dimensions HxWxD 21 x 47 x 35 cm 8 75 x 19 x 14 in Weight 5 5 kg 12 Ib PPS 2 Mini Frame System mainframe power supply and CM 1 Control Panel PPM 2 Modules not included Application Note Working Unit Mini Frame Power Supply and Control Panel plu
48. is 66 mm for mounting on all microscopes through a series of ringlight adapters listed on facing page Order Product W3 72 0238 Standard Ringlight with 750 mm 30 in flexible length W3 72 0239 Standard Ringlight with 1200 mm 48 in flexible length W3 72 0240 Standard Ringlight with 2400 mm 96 in flexible length Made in the USA with some components from Volpi AG Compact Ringlight Full circular glass fiber ringlight provides homogenous shadow free illumination for working distances of 15 to 70 mm Inside diameter is 66 mm for mounting on all microscopes through a series of ringlight adapters listed on page 347 Standard Flexible length is 750 mm 30 in Longer lengths available Made in the USA with some components from Volpi AG Order Product W3 72 0233 M10 Compact Ringlight 4 Point Standard Ringlight Standard ringlight with four fiber points provides illumination with depth perception for working distances of 70 to 100 mm Inside diameter is 66 mm for mounting on all microscopes through a series of ringlight adapters listed on page 347 Standard Flexible length is 750 mm 30 in Longer lengths available Order Product W3 72 0248 4 Point Standard Ringlight Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 345 SUE SJO EUIWIN I ar D o lt 346 Fiber Optic Ringlights continued e Small diameter e Full cir
49. is required use a sapphire blade which provides slices as thin as 6 um All Vibratomes can work with standard glass sapphire or injector knife holders Typical applications include Enzyme histochemistry tissue pathology autoradiology water soluble compounds immunocytochemistry immunohistochemistry fluorescent microscopy tissue pathology botanical studies preparation step prior to Electron Microscopy invaluable teaching aid etc Five different Vibratomes available to fit your applications and your budget e The world standard for tissue sectioning systems for 30 years e Cuts fresh or fixed tissues without distortion e Patented vibrating blade principal e Sections as thin as 6 um using sapphire blade e Less damage than oscillating blade systems e Automatic and refrigerated models available e Simple and quiet operation e Easily maintained e Secure specimen holding vice e Stable blade holder insures no unintentional setting changes e Cool lamp magnifier minimizes heating effect and provide shadow free illumination Vibratome 1000 Classic The original Vibratome semi automatic fresh or fixed tissue sectioning system has maintained a long reputation as a rock solid tissue sectioning system Researchers from around the globe have relied on the Vibratome 1000 Classic for their fresh tissue sectioning needs for over 30 years Vibratome 1000 Plus The Vibratome 1000 Plus utilizes the same mechani
50. ity retention 0 to 50 nanoamperes ejection 0 to 500 nanoamperes The actual current and polarity is continuously displayed digitally and can be externally monitored at the analog output terminal Operating Modes Ejection Timing and Mode Switch The mode switch provides five push button controls which include operations such as cycle trigger gate continuous and termination Cycle Mode In the cycle mode by virtue of selecting single or recycle operation on the BM 2 Module an incoming trigger or cycle start push button will initiate the current ejection pumping action In this mode each succeeding Pump Module is automatically triggered after the pause time of the preceding event has been completed Both eject and pause times can be preset to cover a range from 10 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second resolution Trigger Mode When the TRIG switch is energized the eject time interval will be started by virtue of the incoming trigger pulse applied to the respective Inputs on the BM 2 Balance Module Eject timing interval can be preset covering a range from 10 to 990 milliseconds with a 10 millisecond resolution and 1 to 99 seconds with a 1 second resolution Gate Mode When the GATE switch is energized the eject current will be started by virtue of a gate input signal applied to the respective trigger gate terminals on the BM 2 Balance Module The eject and pause time settings are not
51. jection schedule for each pump can be independently programmed for sequential or simultaneous output Ejection cycles can be internally timed triggered manually or synchronized to external events This flexible system allows the use of iontophoretic pump modules pneumatic pump modules or a combination of both By interchanging the IP 2 lontophoresis Pump Module with a PPM 2 pneumatic pressure pump the overall system capability can be expanded for simultaneous pressure and iontophoretic injection of drugs from a multibarrel pipette The Neuro Phore BH 2 System was developed under the guidance of active researchers with extensive experience in iontophoresis techniques These researchers needed a system to provide precise stimulation and quantitative control for ejection of drugs in their pharmacological studies of drug evoked responses such as neurosynaptic discharges contraction and changes in chemical concentration What emerged was a reliable accurate easy to use Neuro Phore BH 2 system that is capable of accommodating high impedance multi barreled micro electrode pipettes Digitally controlled eject and pause timing utilizes easy to read and set digital panel switches on each iontophoretic or pressure module channel Unbalance and out of compliance indicators are a great aid in troubleshooting clogged pipettes and other problems External analog input control allows external programming of complex or closed loop injection pr
52. llic objects Can be used with Standard Compact and 4 Point Standard Ringlights illuminators Order Product W3 72 0262 Ring Polarizer Set for Standard Compact Ringlights W3 72 0265 Toric Lens for Standard Compact Ringlights W3 72 0264 Diffuser for Standard Compact Ringlights Tools gt D jaa D Note All Volpi Light Sources are compatible with all Volpi Fiber Optic Components Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 347 SJO EUIWIN I SUE a es lt 348 Gooseneck Light Guides Gooseneck Light Guides Gooseneck light guides provide fiber optic incident illumination using semi obedient self supporting arms with no spring back action You can position each arm independently and with the key system in place there is no rotation Single Arm and bifurcated Goosenecks are available with 4 7 mm and 5 4 mm diameter Special Volpi Patent Sliding Sleeve allows fiber bundles to move independently of the outer tubing for minimal fiber breakage over time Gooseneck Arms are repairable Light sources are sold separately Order Product W3 72 0268 Single Arm Gooseneck Light Guide 4 7 mm x 500 mm fiber diameter W3 72 0309 Single Arm Gooseneck Light Guide 5 4 mm x 500 mm W3 72 0267 Bifurcated Gooseneck Light Guide 4 7 mm x 500 mm fiber diameter each arm W3 72 0266 Bifurcated Gooseneck Lig
53. lontophoresis and Micro Injection continued PPS 2 Mini Frame The rack mountable PPS 2 Mini Frame was designed as a less expensive alternative to the BH 2 for those applications that require pressure injections only The PPS 2 is a multichannel pneumatic pumping system designed especially for short term pressure ejection of small quantities of fluids through micropipettes The system can operate with up to four PPM 2 Pump Modules Each module can be programmed to its own schedule of ejection and pause times coordinated with the other modules Ejection and pause times cover a range of 10 to 990 milliseconds with 10 millisecond resolution and 1 to 99 seconds with 1 second resolution Continuous and cyclical modes of operation are also available System Configuration The PPS 2 system Order W3 65 0606 includes a Control Module and a power supply It is pre wired to accept up to four PPM 2 Pneumatic Pressure Modules Order W3 65 0604 which are ordered separately The Control Module CM 1 includes a time clock Panel mounted push buttons as well as TTL electrical inputs are provided to control the following functions Cycle Start Cycle Stop Trigger each PPM 2 and Gate each PPM 2 In essence the CM 1 Control Module provides all necessary signals to operate the sequential and single shot timing for the installed PPM 2 Pneumatic Pump Modules A Self Cycle mode control switch controls independent self timing act
54. mount has top halogen and bottom fluorescent illumination The boom stand does not have any illumination but permits working with large objects You may also order the boom stand separately at a later date should you require it The Microscope is supplied complete with the following components WF10X eyepiece Trinocular zoom body zoom range 0 7x 4 5x Video adapter with external C mount adjustable W3 69 3102 Trinocular Zoom Stereo Microscope With Boom Stand and Video Adapter Specifications Eyepieces Widefield eyepieces of 10x and 25x Objectives Built in zoom and attachable auxiliary lens Zoom Range From 0 7x to 4 5x Illumination Top halogen and bottom fluorescent standard Video Capabilities mount only Built in video and photographic port lens and adapter required Shipping Dimension W3 69 3101 W3 69 3102 490 x 390 x 275 mm 19 x 15 x 11 in Box 1 520 x 420 x 140 mm 20 x 16 x 5 5 in Box 2 300 x 250 x195 mm 12 x 10 x 8 in Shipping Weight W3 69 3101 W3 69 3102 Order W3 69 3101 W3 69 3102 W3 69 3103 W3 69 3104 W3 69 3105 W3 69 3106 W3 69 3107 W3 69 3108 W3 72 4666 W3 69 3111 9 5 kg 21 lbs Box 1 16 5 kg 36 Ibs Box 2 2 5 kg 6 5 Ibs Product SZM 180 Trinocular Zoom Stereo Microscope with Illumination Stand and Video Adapter SZM 180 Trinocular Zoom Stereo Microscope with Boom Stand and Video Adapter 2x Auxiliary Lens for Zoom Stereo Microscopes 0
55. nd two noise reduction chambers on the pressure side ensure absorption of the noise The compressor is also supplied with rubber feet preventing vibrations from imparting to the mounting bolts and foundation The noise level of the oil lubricated compressor is 45 dB A below the noise level of a refrigerator The oil minimizes the wear and tear of the vital parts in the compressor prolonging the lifetime and at the same time ensuring low maintenance costs Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 120 or 230 VAC specify at time or order 6 2A 120 VAC 2 9A 230 VAC 0 54 HP 0 40kW 60 l min or 2 12 CFM 32 l min or 1 13 CFM 8 bar 120 psi 4 liters 1 1 gallon 45 dB A 382 x 300 x 334 mm 15 x 11 8 x 13 2 in 23 kg 50 6 Ib One year parts amp labor Product Ultra low noise 120VAC Ultra low noise 230VAC Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection Harvard Neuro Phore BH 2 System In the past few decades the application of drugs and other dissolved agents from multibarrel electrodes pipettes has evolved into a practical method of testing their effects on cells or cellular systems The versatile Neuro Phore BH 2 System is designed to facilitate controlled ejection of fluids from multibarrel micropipettes Extracellular ejections of minute volumes can be delivered using up to five pumps in serial or parallel The e
56. neck accessories such as Focusing Lens W3 72 0269 Polarizing Caps W3 72 0304 and Modular Accessory System found on pages 348 and 350 Light sources are sold separately Order Product W3 72 0329 Incident Gooseneck Light Guide 4 7 mm fiber diameter each arm 75 cm 30 in W3 72 0330 Incident Gooseneck Light Guide 4 7 mm fiber diameter each arm 100 cm 40 in Incident Gooseneck Light Guide 4 7 mm fiber diameter each arm 150 cm 60 in W3 72 0333 Incident Gooseneck Light Guide Adapter for 20 to 25 mm Round Columns W3 72 0334 Incident Gooseneck Light Guide Adapter for 32 mm Round Columns W3 72 0335 Incident Gooseneck Light Guide Adapter for Nikon SM5 6 and SMZ 6 10 W3 72 0336 Incident Gooseneck Light Guide Adapter for Olympus SZX9 12 W3 72 0308 Incident Gooseneck Articulated Arm Support Adapter for Leica G26 MS5 M26 M28 M212 W3 72 0331 Flexible Light Guides Made in the USA e Point illumination e Highly flexible e PVC metal tubing provides durability e 3 to 13 mm fiber diameters e Standard and custom lengths available Flexible Light Guides Highly flexible and durable light guides available in 3 to 13 mm of fiber diameters and various standard and custom lengths Outer tubing is composed of PVC metal tubing to provide both flexibility and protection standard single arm and twin arm versions available Customized versions are available upon request Flexible Light Guides Fiber Diameter mm 3 5
57. ng Lamp Fluorescent Tube Specimen Adhesive Razor Blades Spare Fuse and Operating Instructions All Vibratomes have an Adjustable Specimen Angle Range of 0 50 degrees and can be used with Standard Glass Sapphire or Injector Knife Holders Other optional accessories include Rotating Stage Assembly Specimen Mounts V Block Adapter Section Mounting Device Specimen Tray Sapphire Knife Glass Knife Adapter and Specimen Retrievers Vibratome 3000 Deluxe Yes Yes Yes 15 mm Yes 5 um 60 x 40 x 15 mm 0 5mm s 5 0 mm s max 40 mm Yes Standard Standard 1 um tissue sampling Yes Yes Yes Yes Yes 2 0 mm Standard gt D i jaa D Yes Yes Standard Yes Yes Yes Yes W3 69 0444 W3 69 0445 Huijdwes anssiy a 1i a Zi 336 Vibratome Tissue Sectioning System Accessories Order Product W3 69 0368 Replacement Fluorescent Tube W3 69 0376 Specimen Adhesive 1 3 oz Bottle W3 69 0377 Blade Single Edge Injector pkg of 10 Vibratome Care Package Order Product W3 69 0382 The Vibratome Care Package Includes 10 Vibratome Feather Blades 1 bottle of Specimen Adhesive 3 Specimen Mounting Blocks a Specimen Tray 2 fuses and 1 Spare Fluorescent Bulb Vibratome The Works Order Product W3 69 0383 The Works Includes everything from the Vibratome Care Package plus Rotating Stage 0 5 inch Specimen Mounts Replacement Rotating Mounts
58. ng blade principle moves the sectioning blade edge in a reciprocating arcuate path as it penetrates the specimen The reduced effective edge angle from the transverse movements with the lateral distribution of the cutting edge penetrating pressure minimize elastic deformation of soft tissue specimens that are simply held or capsulated in position during the sectioning operation Uniform sections can be made of delicate soft tissue specimens Sections are free of observable compressive distortion in the direction of cut as would be typical with conventional microtomes using oscillating blade actions Preservation of ultrastructure is maintained in the sections from a lack of cellular damage on their surfaces Sectioning takes place in a liquid bath The liquid serves to lubricate the sectioning blade to maintain specimen temperature enhance or preserve desirable characteristics of the specimen and facilitate the easy retrieval of the sections generated Using the technique described the Vibratome series of microtomes deliver superior performance using ordinary injector type razor blades Sectioning of uniform fresh tissue sections can be cut from 20 um up although thinner sections may be realized depending on the type of tissue involved and operator technique For conventional use expensive blades made from exotic materials are not required cutting the costs of normal operation and maintenance If a more precise and finer tissue slice
59. nt in nA In this mode automatic current neutralization is provided Automatic balance feature is switched off display reads amount of current in nanoamperes passed through balance barrel as adjusted by pump control In single cycle mode start switch or external trigger initiates each cycle In recycle mode once start switch or external trigger is actuated repetitive cycles commence automatically Two basic time units can be selected 10 msec or 1 sec In 10 msec position Eject and Pause time switches of IP 2 Modules can be set to cover time range from 10 to 990 msec with 10 msec resolution In 1 sec position time scale is expanded from 1 to 99 sec with 1 sec resolution Cycle start stop trigger gate 1 through 5 banana jacks terminals floating input optically coupled input voltage 5 to 15 V TTL compatible Lemo miniature receptacle ground referenced 5 mV nA input impedance 100 kQ Lemo miniature receptacle 5 mV nA ground referenced Lemo miniature receptacle TTL pulse 7 pin miniature connector mates with ultra flexible cable leading to micro electrode holder 21 x 47 x 35 cm 8 75 x 19 x 14 in 8 2 kg 18 Ib 125 V at 0 1 A 15 V at 0 5 A 5Vat3A Line operated 115 to 220 VAC 50 60 Hz 13 x 47 x 35 cm 5 1 4 x 19 x 14 in 11 kg 24 Ib Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Harvard Neuro Phore BH 2 System Micro
60. nto the micropipette following an injection 3 Clear a micropipette of material in it Unique Features The PLI 100 s important Balance Hold and Clear functions are not found on other microinjection units Balance In addition to the ejection pressure the Pico Injector offers a secondary balance pressure This secondary balance maintains a positive pressure on the injection pipette before and after injections This eliminates dilution caused by capillary action and aids in the prevention of clogging Wasteful continuous injection which often occurs when no separate balance pressure is offered by an injection device is avoided Fill Hold There are two built in vacuum generators to fill a micropipette from the tip and to hold suspended cells Filling the pipette from the tip is easier than back filling Suspended cells can be held with a second holding pipette The holding vacuum s range accommodates most cell types Clear A high pressure pulse can be used for clearing a pipette should it happen to clog This is particularly useful when working with pipettes sized for smaller volumes Electrical Connectors BNC type connectors are available at the front panel to ease integrating the Pico Injector with other equipment Synchronization of injections to other stimulations or recordings is therefore possible Selection Guidelines The PLI 100 is available in three packages Plus Basic and Deluxe The basi
61. nts W3 72 0324 W3 72 0322 W3 72 0325 or W3 72 0326 depending on light grid brushing g Order W3 72 0323 W3 72 0324 W3 72 0325 W3 72 0326 Product Articulated Support Arm M6 M6 Thread Heavy Steel Base for Articulated Support Arm Holder for Articulated Support Arm ferrule diameter 9 to 12 mm 0 35 to 0 47 in Holder for Articulated Support Arm ferrule diameter 11 to 16 mm 0 43 to 0 63 in Modular Accessory System e Use with Goosenecks and up to 8 mm OD Flexible Light Guides e Allows combination of filters and other accessories e Very versatile Modular Accessory System Modular Holder has a 10 mm mounting diameter and attaches onto ends of goosenecks and up to 8 mm OD flexible light guides Modular Accessory Holder and each of the Modular Accessories are threaded to accept one another Modular accessories include the Modular color filters Modular focusing lens Modular diffuser Modular polarizing filter and Modular iris diaphragm One or all can be attached at any one time Custom filters are also available upon request Order W3 72 0270 W3 72 0271 W3 72 0272 W3 72 0273 W3 72 0274 W3 72 0275 W3 72 0276 W3 72 0277 W3 72 0278 W3 72 0280 W3 72 0279 Product Modular Accessory Holder Modular Focusing Lens Modular Iris Diaphragm Modular Polarizing Filter Modular Daylight Filter Modular Blue Filter Modular Red Filter Modular Green Filter Modular Yellow Filter Modular Blank Filter Modular Diff
62. oliters e Easy to use e Economical e Precise The PLI 90 Pico Injector like the reliable PLI 100 Pico Injector controls the precise and reproducible regulation of injection pressure and time Simplicity Because the PLI 90 features only the injection balance and clearing pressures It is a lower cost alternative to the PLI 100 This simplicity makes it even easier to use than the PLI 100 Selection Guidelines The PLI 90 is ideal for the user who does not require vacuum for filling pipette barrels or if using a holding pipette for holding cells is not essential to your application The PLI 90 is available in two packages Basic and Plus The basic model includes an input and output hose handle power cord and instruction manual The plus model is supplied with all of the same parts as the basic model but also includes a Footswitch Pipette Holder and Input Hose Adapter Specifications Input Gas Pressure Injection Pressure Balance Pressure Clearing Pressure Injection Time Pressure Display Duration Mode Trigger Mode Pressure Readout Line Voltage Power Usage Foot Switch es Accessories Supplied Weight Dimensions H x W x D Order Model 70 to 105 p s i 480 to 720 kPa 0 2 to 60 p s i 413 kPa regulated multi turn control 0 1 to 3 5 p s i 68 9 kPa regulated multi turn control other ranges available upon request Input gas pressure unregulated 0 01 to 0 99 sec in 10 msec steps 1 to
63. olpi AG Warner Instruments Phone 203 776 0664 e Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Ringlights and Accessories e Ergonomic e Long working distance starting at 100 mm e Direct mounting to Olympus Ergo Olympus SZ SD Ringlight Ergo Olympus SZ SD Ringlight Tapered ringlight for direct mounting on Olympus SZ SD Microscopes with minimum loss of working distance Flexible length exits at 45 angle allowing maximum work space Long Working distance starting at 100 mm with long flexible lengths available Adapter included e ie sta J Order Product W3 72 0242 Ergo Olympus SZ SD Ringlight Made in the USA e Reduces or eliminates unwanted reflections e Extends standard ringlight working distance range e Used with standard compact and 4 point standard ringlights Diffuser Accessories for Standard Compact and 4 Point Ringlights Ring Polarizer Set Eliminates unwanted reflections of highly reflecting materials such as tissue and metallic objects Glass analyzer is used with plastic polarizer for the best possible cross polarization effect Can be used with Standard The Compact and 4 Point Standard Ringlights Made inthe USA Toric Lens Extends the working distance of Standard and Compact Ringlights from 90 to 150 mm Longer distance ringlights are also available as an alternative Diffuser Reduces unwanted reflections of highly reflecting materials such as tissue and meta
64. on e Designed to prepare tissue for metabolic experiments This Mcllwain tissue chopper has been designed to prepare pieces of tissue for metabolic experiments It is especially suited for small and irregular specimens from biopsy or small organs The Tissue Chopper causes much less disturbance of the cell structure than homogenizers or blenders and is applicable to fragments which would be difficult or impossible to cut It has been applied to liver kidney and various parts of the central nervous system This tissue chopper will produce slices up to 1 mm thick Cubes or prisms up to 1 mm in cross section can also be prepared within 30 seconds The stepless variation of slice thickness from zero to the maximum Is controlled by an index clutch in conjunction with a micrometer head The latter being calibrated in microns The action of the machine is as follows a circular stainless steel table on which the specimen is placed is traversed from left to right at a speed which can be varied At the same time a chopping arm carrying a blade is raised and dropped by its own weight and the tension of a spring at speeds varying from zero to a maximum of 200 strokes per minute As the table carrying the tissue passes under the blade it is chopped into the required slices If prisms are required the table is returned to the left hand side of the machine turned through 45 degrees and the process repeated Cubes are formed by rotating the table 90 degre
65. operative in this mode since the eject time function is slaved to the duration of the gate input Continuous Mode When the CONT switch is energized the ejection pump current is continuously maintained Termination Mode When the TERM switch is energized the output is automatically diverted from the preparation into an internal dummy load 100 MQ This function is particularly useful for testing of possible instability in the preparation pipette Analog Input The analog input terminal is available to facilitate externally controlled Current pumping action An external voltage applied to the input will generate a pumping current at a ratio of 5 millivolts nanoamperes This current will be summated with any preset pump current governed by both the retention and ejection controls The combined magnitude and sign of the summated pumping current is displayed on the digital display of each corresponding Pump Module This input can be connected to a computer D A converter when external programming is desired Analog Output The analog output terminal provides a buffered voltage which is proportional in magnitude and polarity to the actual current passed from the current pump into the pipette The conversion ratio is 5 mV nA This output can be polygraphically recorded to monitor progress of the experiment Sync Output The sync output provides a TTL pulse that coincides with the eject time This output is provided to trigger extern
66. otocols Current and electrode resistance readout with large bright easy to read digital displays System Configuration Balance Module The Neuro Phore BH 2 System is modular and is comprised of the S following components all ordered separately 3 e Used by leading researchers for over 20 years One BH 2 Mainframe Chassis W3 65 0600 that can accommodate 1 to e Modular 5 lontophoretic Pump and or Pressure Pump Modules Mainframe O e Minimal noise Chassis can be rack mounted or set on table e Up to 6 channels One BM 2 Control and Balance Module W3 65 0602 z e Digitally controlled eject and pause timing P 2 lontophoretic Pump Modules W3 65 0603 and or E e Mix and match pump types PPM 2 Pressure Pump Modules W3 65 0604 a One low noise MS 2 Power Supply W3 65 0601 amp Features Blank Panel DP 1 Dummy Module W3 65 0605 used to maintain Successfully utilized in key laboratories around the world for over 20 timing if less than 5 Pump Modules are installed years with many journal articles published as a result One Model OC 01 output cable W3 65 0215 Modular design buy what you need now and add additional modules One Model NL 952 lemo cable W3 65 0285 later as your protocol evolves Accommodates 7 barrel micropipettes supports the most complex and demanding injection and recording protocols Extremely low noise 105 volts compliance allows rapid iontophoretic injection with even the smallest micropipette
67. ration This function is particularly useful for setting up the desired pressure range and timing while preventing ejection of any drug from the pipette Specifications Source Gas Input Pressure Input Filter Output Pressure Output Pressure Display Air or Nitrogen recommended no explosive or combustible gases 125 p s i g 7 8 kg cm2 maximum 5 um element 0 to 99 9 p s i g 0 to 7 kg cm2 30 p s i standard Three decimal digits Pressure Pulse Width Minimum 30 msec Maximum 99 sec 990 sec optional Gas Input and Quick disconnect type Output Couplings Lemo miniature connector voltage proportional to output pressure 0 to 999 mV full scale in p s i g setting 0 to 700 mV full scale in kg cm2 setting Analog Output Lemo miniature connector TTL pulse time incident with output pressure pulse Red LED Green LED Sync Output Eject Time Indicator Pause Time Indicator Order Product W3 65 0604 PPM 2 Pneumatic Pump Module with Input Output Hose microinjection 7 Barrel lontophoresis Micropipette The MS 7P Micropipette has been designed for iontophoretic use as well as pneumatic ejection of drugs The standard blank consists of seven barrels Pyrex Omegadot tubing 1 5 mm outer diameter and 1 2 mm inner diameter pre pulled to 2 mm formation as shown For complete details see our Warner Instruments Cell Biology Catalog gt D co D Order Model W3 65 0207 MS 7PB
68. roinjection gt D jaa D 323 uo dafulo1IW a 2 2 lt 324 Model Air 1 Ultra Low Noise Air Compressor Specifications Input Voltage Range Maximum Current Motor Displacement FAD 8 bar Max Pressure Tank Size Noise Level Physical Dimensions Size HxWxD Weight Warranty Order Model W3 64 1701 AIR 1 U W3 64 1702 AIR 2 e Suitable for installation directly at point of use e Lowest noise and vibration level in the market 45dB A you won t even know it s on e Complete compressed air package e Ideal for use with our microinjection systems When a reliable supply of clean and quiet compressed air is required this oil lubricated compressor is the perfect choice The noise level is 45 dB A far below the level of normal conversation Quiet vibration free and reliable this compressors compact design easily allows mounting at the place of use The oil lubricated piston compressor is supplied ready for use The unique synthetic SJ 27 oil designed especially for AIR 1 optimizes the lubrication of the compressor Furthermore the internal motor is mounted in a closed motor house reducing the noise level even further Due to the design of the motor the oil is also used for cooling the motor The motor is mounted on springs inside the motor housing which means that hardly any vibrations are imparted to the surroundings Two noise reduction chambers on the intake side a
69. roinjection range from assisted In vitro cell fertilization techniques to the transport of molecular and cellular elements These substances are typically injected into the cell to manipulate and or monitor the fundamental biochemistry of a specific living cell Substance that can be injected include cellular organelles kinases histochemical markers such as horseradish peroxidase or lucifer yellow proteins metabolites microbeads ions antibodies genes molecular biology mRNA and DNA etc The precise delivery microperfusion of small volumes picoliter to milliliter of various agents and drugs to a cell or group of cells for applications such as pharmacological drug testing can also be accomplished using these techniques In order for the researcher to conduct any of the above experiments and get meaningful results the tools equipment used in these experiments must provide not only the specific functionality but they also need to be of the highest quality and provide the necessary reliability accuracy and repeatability to insure proper results Harvard Apparatus Inc manufactures and sells the complete range of product that are needed to successfully microinject and or microperfuse uoNdafulo1sIW Abojoig ATEH Pipette Pullers pages 243 and 245 Micromanipulators pages 267 to 276 318 Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com PLS 1 Pico Inj
70. s e Ringlight adapters available for all brand microscopes e Provide direct and indirect mounting e Low Cost Ringlight Adapters Used with Standard Compact and 4 Point Standard Ringlights for attachment to all brand microscopes If your microscope is not listed here please contact customer service for assistance Order W3 72 0249 W3 72 0250 W3 72 0305 W3 72 0251 W3 72 0252 W3 72 0257 W3 72 0258 W3 72 4666 Product Ringlight Adapter for Leica Classic Stereo Zoom 1 5 without Attachment Lens Ringlight Adapter for Leica New Family SZ4 SZ6 Photo Plus without Attachment Lens Ringlight Adapter for Leica New Family SZ4 SZ6 Photo Plus with Attachment Lens Ringlight Adapter for Leica Stereo Zoom 7 GZ7 without Attachment Lens Ringlight Adapter for Leica M3B C Z Leica MZ6 Achromatic 0 32x 0 5x 0 63x 0 8x 1 0x Ringlight Adapter for Olympus SZ without Attachment Lens Ringlight Adapter for Olympus SZH9 SZH12 and Nikon SMZ Series without Attachment Lens Ringlight Adapter for SZM 180 Stereo Zoom Microscopes see page 338 800 599 4203 Fax 203 776 1278 www warneronline com illuminators gt D jaa D i 343 SJO BUIWIN I SUE a ee lt 344 Fiber Optic Ringlights White LED Ringlights atGe aa a a a a a a o o gt il ary E k s TTS ia a T CE Made in the USA with some components from Volp
71. s four 4 modules selected from PPM 2 and DP 1 Order Product W3 65 0606 PPS 2 Mini Frame with Power Supply Control Module and NL 952 Power Cord and Manual W3 65 0604 PPM 2 Pneumatic Pump Module with OH 01 and IH 01 PPS 2 System Can Support 1 to 4 Modules W3 65 0605 DP 1 Dummy Module to Maintain Timing PPS 2 System Can Support 1 to 4 Modules W3 65 0210 Model SC 01 Tygon Flexible Hose Micropipette Interface Coupling for Pneumatic PPM 2 Use microinjection W3 65 0211 Model SC 02 Tygon Flexible Hose Micropipette a Interface Coupling for Current IP 2 Pneumatic PPM 2 Use on W3 65 0212 Model IH 01 Input Hose 2 7 m 9 ft Coupling amp to PPM 2 W3 65 0213 Model OH 01 Output Hose 2 7 m 9 ft Including SC 01 Coupling W3 65 0214 Model OH 02 Output Hose 2 7 m 9 ft Including SC 02 Coupling W3 65 0215 Model OC 01 Output Cable 2 7 m 9 ft with 7 Seven Pin Lemo Connector W3 65 0285 Model NL 952 Cable 2 m 6 5 ft with Lemo Miniature Connector at One End and Tinned Leads at Other Sync Output or Analog Input Output Cable Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 327 uoNdafulo1IW O eh 2 lt 328 Harvard Neuro Phore BH 2 System Micro lontophoresis and Micro Injection continued IP 2 lontophoresis Pump Module Each IP 2 Module includes controls for precise settings of current magnitude and polar
72. specimen bath The refrigeration is a great benefit for those researchers trying to maintain constant temperature and constant chemical makeup of buffer solution Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Vibratome Tissue Sectioning Systems continued Specifications New Zero Z Technology Adjustable Frequency Dynamic Stability System Total Vertical Specimen Stroke Adjustable Sectioning Window Section Thickness Increments Maximum Specimen Size Sectioning Speed adjustable Return Stroke Speed Maximum Sectioning Range Drain New Deluxe Dual Bath System Multi Specimen Sectioning Repeatable Height Adjustment Use Disposable Microtome Blades Rotating Specimen Mount Stroke Pause Switch Separately Adjustable Light and Magnifier Arcuate Blade Path Adjustment Foot Switch Fully Automatic Operation Insulated Bath Built In Refrigeration Cumulative Section Counter Full Text LCD Display 0 01 Increment Sections Specimen Auto Bypass 110 VAC 220 230 240 VAC Vibratome 1000 Classic No No No 10 mm No 10 um 25 x 25 x 10 mm 0 1 25 mm s 1 25 mm s 30 mm No Optional Optional 10 um Yes Yes No No Yes 1 25 mm No No No Optional No No No No W3 69 0353 W3 69 0354 Zero Z technology includes an adjustable frequency mode Warner Instruments Phone 203 776 0664 e Toll Free U S 800 599 4203
73. syringe series including the 1000 500 200 100 and 50 ul sizes visit www harvardapparatus com for complete details gt D 2 jaa T i Order Product W3 69 0107 Screw Actuated Micrometer Driven Hamilton Syringe Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com 331 uo dafulolIW Abojoig TEH 332 PM 8 and PM 4 8 and 4 Channel Pressure Injection Systems W3 69 0153 8 Channel Pressure Injection System Hann g ELLE HIS oann fag e Multiple function system for micro injection and perfusion e Up to 8 injection perfusion outputs one hold cell output and one synchronized drain out channel e Programmable timers counters and step sequences which can be stored for reuse e Manual control or automatic sequential step cycle operation e Front panel display for pressures and programming information The PM 4 and PM 8 programmable 4 and 8 Channel Pressure Injector Systems are designed for one to eight channel intracellular injection and extracellular perfusion These microinjection and perfusion engines are especially designed for pharmacological drug testing molecular biological DNA RNA transferring intracytoplasmic sperm injection and cell electrophysiological applications Combining an advanced micro controller with precision pneumatic components these systems can simultaneously control up to eight injection micropipettes or e
74. ttained After the tissue has been cut into slices it may if desired be turned 90 degrees and formed into cubes after passing under the cutting arm again The machine automatically switches Itself off at the end of its travel When this limit switch is used it must be reset by means of the top button The cutting arm is made of stainless steel and may be removed for the purpose of sterilization 5 Plastic Cushion This machine is now fitted with a plastic cushion held in place by two spring clips on the table The specimen may be fixed to a suitable filter paper by means of a fixative such as agar which in turn is cemented to the cushion and then inserted under the springs This arrangement holds the specimen firmly down and prevents It sticking to the blade during chopping Specifications Maximum Blade Height 1 cm Slice Thickness Settings 1mm maximum with 1 um scale resolution Minimum Section Thickness Blade Speed Voltages Dependent on tissue type Zero to 200 strokes min 90 120 VAC 60 Hz or 210 240 VAC 50 Hz 30 x 28 x 15 cm 11 8 x 11 0 x 5 9 in 6 3 kg 13 9 Ibs Overall Dimensions Weight Order Product W3 69 1010 Mcllwain Mechanical Tissue Chopper 115 V 60 Hz Mcllwain Mechanical Tissue Chopper 230 V 50 Hz Replacement Parts W3 69 1012 Cutting Blades pkg of 10 W3 69 1014 Cutting Table Disk PVC Darvik pkg of 10 W3 69 1015 Replacement Cutting Table W3 69 1016 Cutting Table Clips W3 69 1017 Sp
75. ulti channel injection perfusion engine Connection Diagram for Extracellular Micro Perfusion and Intracellular Micro Injection Systems PM 4 8 Programmable Pressure Injector 30 to 100 psi a Pressure Source TS PE C Hold Cell Vacuum PMA 4 Channe Injection PMB 8 Channel Injection Pressure Output Drain Out Liquid oR 7 ae D Pipette Single Barrel Injection Pipette Tested Cell Extracellular Micro Perfusion Connection FSW Foot Switch PM KP _L Injection Time Signals Remote of 4 or 8 Channels Key Pad 4 an a ee vl AN ii HANN Hold Cell Pipette LU cUu N X aS h Il AH Hold Cell Pipette angie namel Injection Pipette Intracellular Micro Injection Connection Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com PM 8 and PM 4 8 and 4 Channel Pressure Injection Systems continued W3 69 0152 4 Channel Pressure Injection System Below are the components supplied with each of the Pressure Injection Systems Pressure Injection System PM 4 PM 8 Order Product Oty Oty W3 69 0154 IP1 Input Tubing with Connector 1 1 W3 69 0156 OP2 Output Tubing with 6 10 Connector Only W3 69 0157 FSW Foot Switch 1 1 W3 69 0164 PP 4 Pulled 4 Barrel Pipette 1 W3 69 0166 HOLD4 Holder for 4 Barrel Pipette 1 W3 69 0163 PP 7 Pulled 7 Barrel Pipette 1 W3 69 0167 HOLD Holder for 7 Barrel Pipette
76. user Note All Volpi Light Sources are compatible with all Volpi Fiber Optic Components Warner Instruments Phone 203 776 0664 Toll Free U S 800 599 4203 Fax 203 776 1278 www warneronline com Backlights and Base Glass Fiber Backlights 12 7 cm 5 in Backlight Made in the USA 5 cm 2 in Backlight Made in the USA e Homogeneous diffuse backlight illumination e Used in the image processing brightfield illumination on stereo microscopes pattern recognition of different transparencies area recognition of different colors side illumination dimensional gauging shape verification etc Glass Light Fiber Backlights Glass Fiber Backlights provide homogeneous diffuse illumination Glass fiber used is extremely durable and heat tolerant Can be adapted to most brand microscope bases Available in three sizes 5 x 5 cm 2 x 2 in 7 6 x 7 6 cm 3 x 3 in and 12 7 x 12 7 cm 5 x 5 in Additional specifications listed below Specifications W3 72 0342 W3 72 0343 W3 72 0344 Product 2inch Backlight 3inch Backlight 5 inch Backlight Homogeneous 5x5cm 7 6 x 7 6 cm 12 7 x 12 7 cm Illumination Field 2 x 2 in 3 x 3 in 5 x 5 in Dimensions HxWxD 1 3 x 10 2 x 6 4 cm 2 3 x 17 2 x 12 1 cm 2 3 x 22 2 x 17 2 cm 0 5 x 4 0 x 2 5 in 0 9 x 6 75 x 4 75 in 0 9 x 8 75 x 6 75 in Uniformity 10 10 10 Fixing Holes 4 each 6 3 mm 0 25 in 6 3 mm 0 25 in 6 3 mm 0 25 in Diameter Flexible Lig

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