User s operating notes
Contents
1. LabRam HR Raman Microscope User s Operating Notes Important This is not a comprehensive guide for use of the LabRam HR Raman Microscope and not a User s Manual of the LabRam HR Raman Microscope This is short reminder notices and operating tips for references only made by other users Training is required before using the LabRam HR Raman Microscope To obtain training contact the Characterisation Lab Manager IAI AIA AA A AS A AS AS AS AS AS AS AS AS AS AS AS AS AS AS AI AS AS AS AS AS AS AS AI AS AS ASAI AI ASI ASI AI AI ASI ASI OS Microscope Spectrometer i Electronics MEGRUN aa Box rife pi gt y TIELEN G hb etb be eS et eee iPadted SAMEINI ee SETETEST aJe TII CERTEGE mebouye if is Th i ire ire ee ee Control Box Electronics Box Leave on at all times The piezo attachment is switched on to this box Control Box provides an interface between the user and the instrument leave it switched on all the time When the power supply is turned on a red light will be lit on top left hand side of the front of the box ans res PEt CANNER CAMERA iy hi PALS Ee gt t FA mr alata ON ON ON SHUTTER 4 z 9 O gown up down up down xa Up E wreriocx moicaror HORIBA OBIN YVON e Laser OFF Turn key to switch laser on amp off When laser switched on the two small LED Laser amp Interlock indicators will glow red e DIODE ON Always leave OFF If you leave this ON the CCD will sa2. length set hole 100 um filter to reduce the power usually 10 set 2400 grading this parameter affects on the noise and intensity set proper lens which is chosen in the microscope Zero Calibration check beam splitter switch set to video beam splitter down in the manual turn on white light box change units to nm Options gt Units gt nm click arrow left in the window spectrometer to send spectrometer to zero position wait until you see O gt Il lt in the window spectrometer click the blue wheel to take the spectrum press Stop when spectrum is taken zoom in on peak using magnification button looks like magnifying glass go to Setup gt Instrument Calibration wright on the paper values shown in the two bottom windows if the peak position not at zero adjust Zero Parameter in small amounts up to 5 press Apply when peak at zero resend spectrometer to zero 0 gt l lt again and retake spectrum Raman Calibration change units to cm Options gt Units gt cm send spectrometer to 520 7 cm position put the Si calibration sample on a glass slide under the microscope objective click Camera icon to open the video window and focus on silicon calibration sample x100 objective click Stop turn off the white light then switch the beam splitter to Raman move up the laser on switch on the Control Box click the blue wheel to take at 25 300 um h
3. ole press Stop magnify peak as before go to Setup gt Instrument Calibration and adjust KOEFF value by small amounts until peak is in correct position press Apply retake spectrum check laser power approx 5 0 mW modify filter accordingly To take spectra go to extended Range and set Multiwindows enter bottom and top value for the required diapason To switch off the blue green laser Switch off the Argon Power Supply under the table switchthe switch on the PCB boarch rom on to standby the D standby will on ETET wait 20 min switch off ne main switch AC power Using UV laser put the warning sign on the door wear UV protectin googles Behind the setup switch on the red switch turn on the key touch the tubes to check cooling From the top move down the mirror set the filter 364 nm at the output use the 60x lens calibrate and take spectra as for visible laser
4. on on the top right hand side of the screen Always remember to turn off the white light illumination power source and to put the camera beam splitter to the RAMAN position before starting a Raman measurement To illuminate by transmission only remove the white light fibre optic from the top left port of the microscope Using green and blue laser Info laser beam spot at 100x lens is about 0 6 um Switch on the Argon Power Supply under the table switch on themaain switch2AC power turn key clockwis 90 LAS Mak irom s wah A APR ES Y switchCthe switch on the PCB board from standby t00 the LED standby will off change two filters marked 1 in the picture under the cover at the top of spectrometer for 457 or 514 nm wavelength check that the mirror marked 2 in the picture is pulled up close the cover adjust the laser to maximum brightness using the top knob behind the setup the top knob click on LabSpec 5 icon on PC You will see in the top the following icons Camera to take video image Blue wheel take fast measurements Pink pig take measurements Ridge take the map Greed set map parameters in the acquisition group of windows at the bottom Top window Refresh time Collect time 5 sec usually Scan number to take an average The centre of the measurements window is set in the window spectrometer choose the wave
5. turate due to too much light getting to it e SHUTTER MICRO If using the microscope turn it to the up position If using fibre optic entrance then turn it to the down position e CAMERA ON This switch controls the power for the camera in microscope Leave ON all the time e LASER ON Shuts laser to come down the microscope towards the sample Leave OFF when not in use Liquid Nitrogen Sa Reservoir Symphony Controller pares Liquid Nitrogen Reservoir is essential to keep system cool at all times it should be filled 3 times weekly Symphony Controller provides co ordination of all detector functions including power distribution array temperature regulation digital readout control and CCD video output conditioning and digitalization of pixel information Microscope light source Joystick moves the motorised table LabspecTango table driver The joystick handle moves the table and moves up and down by its rotating 2 Beam Splitter Image Acquisition The LabRAM is equipped with a standard white light illumination of the sample by reflection and or transmission A colour camera linked to the software allows the sample to be visualised and the image captured on the PC amp saved Put camera beam splitter into VIDEO Turn on the white light illumination power source In the software click on the video icon to start camera read out To freeze picture click on the stop ic
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