Perfusion Valve Controller User`s Manual
Contents
1. 24 blunt end 18 gauge syringe needles and 8 stopcocks A new and exciting feature to the VC 8 Valve Control System is the Spill Sensor Probe This probe is used to detect the presence of an overflow condition and when activated will automatically shut off all valves protect ing your microscope optics The controller can independently regulate the function of up to eight valves Individual valves can be controlled via manual switch an external analog signal or an external digital TTL signal An event marker pulse gen erated each time a valve is switched on is provided at the rear of the instru ment for recording into your acquisition system Valve transitions opened or closed occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions Features e 8 channel individually controlled channels Teflon valves e Spill sensor Manual and computer control Publication 5715 001 REV A a Perfusion Valve Controller Model VC 8T Warner Instruments Operating Directions Setup Components Before beginning setup take inventory of the supplied components You should have e valve bracket with included Teflon valves e syringe holder support stand blunt end syringe needles 24 e stopcocks 8 Teflon tubing 25 ft e PE 160 tubing 10 ft e MPP 8 port manifold e VC 8 Valve Controller syringe holder stopcock2. Teflon tubing VC 8 Valve Controller The VC 8T is designed to operate as a stopped flow device wherein the valve for each channel is either open allowing solution to flow or is closed In general the shortest response time for delivery of the selected solution will be achieved by keeping the tubing length between the MAN IFOLD and sample as short as possible Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments Operating Directions Contd Assembly 1 2 Begin assembly of the VC 8T by first attaching the VALVE BRACKET to the SUPPORT STAND Place the VALVE BRACKET near the base of the SUPPORT STAND as shown on previous page This is followed by attaching the SYRINGE HOLDER to the top of the SUPPORT STAND Place the SYRINGE HOLDER near the top of the SUPPORT STAND as shown on the previous page valve bracket Teflon valves Remove the plungers from the eight supplied 60 cc syringes and place syringes into the SYRINGE HOLDER Attach STOPCOCKS to each syringe Cut eight 8 pieces of Teflon tubing long enough to run from the stopcocks to the input ports on the Teflon valves Attach one 1 blunt end 18 gauge syringe needle to both ends of each length of Teflon tubing to provide Luer connection points Insert a short section of PE 160 onto the needle tip to facilitate a tight seal between the Teflon tubing and the syringe needle Using the Luer ended Tef
3. changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required z Perfusion Valve Controller Model VC 8T Warner Instruments Table of Contents INTOJUCUON ea 2 Fodera 2 Operating Directions a 3 lic 3 CONIDONPNS crea eo tee PU E REO E 3 AS e do 4 Overflow Selene 6 Control Description iii ia 7 Front ra ina i OT e ERI PRA ee 7 Command Input Toggle rii 8 OVenioWslicoba alii 8 Rear P A e ESO A 8 A I RI RR RR CTR 8 A ln 8 Standby IM eas 8 EKMAN ADD Mirta tende 9 O IE a 9 Power Input Module pla 9 AG Conversio ias 10 INSTUCIOAS TOUS sati 10 POW AG ISI elia aa 10 Manten 11 Cei 11 Warranty aa 12 ADPENOD airin 13 JA PPP t 13 Declaration of Conformity ccccncccciciconanicnancanacnancanannannancanannos 14 Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments introduction The VC 8T Valve Control System lies at the heart of a multi valve perfusion sys tem designed to automate and control the delivery of solutions to Warner Instruments imaging and recording chambers However its flexible design allows the VC 8T to be used in many applications not using Warner equipment The complete system includes a valve controller a valve bracket with Teflon valves connecting cable an MPP 8 manifold a support stand syringe holder eight 60 cc syringes 25 feet of Teflon tubing 10 feet of PE 160
4. Perfusion Valve Controller Model VC ST A WARNER INSTRUMENTS A Harvard Apparatus Company CE Publication 5715 001 REV A WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any
5. able Publication 5715 001 REV A gt Perfusion Valve Controller Model VC 8T Warner Instruments Operating Directions Contd Overflow Sensor The VC 8T Valve Control System includes a unique overflow or spill sensor designed to assist you in providing a level of protection to your micro scope The sensor itself is a small Ag AgCl probe which grounds through the chamber bath A resistance of 10 MQ or less between the probe and ground will trigger the overflow circuitry Place the probe adjacent your perfusion chamber such that the maximum solution height say just prior to an actual overflow is achieved when the solution contacts the tip of the probe Contact between the probe and your solution will activate the overflow circuitry and close all the valves The OVERFLOW SENSOR plugs into the OVERFLOW IN BNC on the rear of the controller Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments Control Description Front Panel The front panel of the VC 8 Valve Controller contains TTL inputs for each valve an associated 3 position toggle switch for manually setting the state of each valve and an LED displaying the active status for each valve There is also a 2 position toggle switch for selecting the command input mode a SPILL SENSOR indicator and a power switch with power on LED aaa lo o G TTL Inputs TTL inputs front panel BNC s and ON OFF TOGGLE SWITCHES are pro
6. e Selection C w a i SIN A i ii aL _ a Event Out The Event Marker output produces a 500 ms logic level output 5 V each time a valve is turned on At all other times the Event Marker output is low 0 V Power Input Module A polarized 3 conductor IEC320 CEE 22 connector is used for line mains power input to the instrument A removable cordset terminated with a NEMA 5 15P connector is standard A fuse holder contains a protective fuse in series with the high side brown or black wire of the mains The holder accepts 5 x 20 mm fuses of the type indicated Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments Control Description Conto AC Conversion ATTENTION PLEASE READ BEFORE APPLYING POWER TO YOUR UNIT The unit uses only one fuse the second one is sent as spare Follow these instructions to change the unit s from 120 VAC or 220 VAC Power Entry Module Fuse holder side view Push out drawer to access the Spare Fuse Carefully pry open the fuse holder from the inside by using a small flat screwdriver Replace fuse according to voltage being used For 120 VAC 0 50 Amp 5 x 20 mm Slow Blow For 220 VAC 0 25 Amp 5 x 20 mm Slow Blow Depending on AC Voltage being used turn VAC selector switch to 110 VAC or 220 VAC Instructions for Use Flow Adjustment Flow rates can be adjusted by raising or lowering the reservoir holder as well as by adjus
7. lon tubes formed in step 5 make a connec tion between the stopcock on each syringe to the associated input port on each Teflon valve Proper tubing lengths Luer connector attachments and tubing placements are shown in the montage below Cut eight 8 pieces of Teflon tubing to run from the output ports of the Teflon valve to the supplied MPP manifold The tubing length need ed is left to the discretion of the user but should be sufficient to allow for a short connection between the manifold and the sample chamber Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments Operating Directions Contd 10 11 12 Attach the remaining blunt end syringe needles to one end of each section of Teflon tubing Prepare the MPP manifold to accept the Teflon tubing by first sliding a short length of PE 160 tubing over the input ports on the MPP manifold Now make a connection between the output port on each Teflon valve and one input port on the MPP manifold Run a short length of PE 160 tubing from the output port on the MPP manifold to the input port of sample chamber NOTE If desired you can also run Teflon tubing between the mani fold and your chamber Prepare the manifold to accept the Teflon tubing by first sliding a short length of PE 160 tubing over the out put port on the MPP manifold Finally connect the VALVE MANIFOLD to the VC 8 CONTROLLER using the attached c
8. lty component s at our discretion This warranty does not cover failure or damage caused by physical abuse or electrical stress e g exceeding specified input limits Shipping charges to the factory are the customer s responsibility Return shipping of the repaired unit will be paid by Warner Instruments Inc Publication 5715 001 REV A a Perfusion Valve Controller Model VC 8 Warner Instruments Appendix Specifications Valve Bracket Delrin Mounts on 3 8 or 1 2 ring stand Valves Teflon 12 VDC 0 25 A to maintain pinch Connection Cable 2 4 meter 8 ft connecting cable terminated with quick disconnects on valve end and 15 pin male D type connector on controller end Tubing Teflon 1 8 OD x 1 16 ID tubing Reservoirs 60 cc capacity syringes Reservoir Holder Delrin Holds eight syringes with thumb screws for each reservoir Valve Controller front panel Power Two position on off with power on LED Input Select Two position selecting analog or digital inputs Switch Selection ON manual Off or External External Input 5 V TTL compatible BNC Connector Overflow LED indicating overflow condition Valve Controller rear panel Overflow In BNC connection to OVERFLOW SENSOR Triggered when sensed resistance falls below 10 MO Standby In BNC connection to activate standby mode TTL high closes all valves Ext Analog In BNC connection for valve selection Active valve opened by analog voltage Event Marke
9. r Logic level pulse 500 ms nominal rear panel BNC connector Manifold 8 1 nominal dead space Power 110 to 130 or 200 to 250 VAC single phase 50 60 Hz 20 W Operating Temperature 10 to 40 C 50 to 104 F Dimensions H x W x D 89 x 203 x 305 mm 3 5 x 8 0 x 12 in Weight Shipping Weight 3 7 kg 8 lb 4 6 kg 10 Ib Operating Conditions Equipment is intended to be operated in a controlled laboratory environment Temperature 0 to 40 C Altitude Sea level to 2000 m Relative Humidity 0 to 95 Publication 5715 001 REV A Declaration of Conformity CE MARKING LVD amp EMC Application of Council Directive 73 23 EEC Standard s to which conformity is declared Manufacturer s Name Manufacturer s Address Equipment Description Equipment Class Model Numbers 89 336 EEC EN61010 1 1993 EN55022 Class A EN61000 3 2 EN61000 3 3 EN50082 1 1992 EN61000 4 2 EN61000 4 3 ENV50204 EN610000 4 4 EN610000 4 8 EN610000 4 11 Warner Instruments LLC 1125 Dixwell Avenue Hamden CT 06514 T 203 776 0664 Valve Controller Class I ITE Class A VC 8 Controller I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s WARNER A INSTRUMENTS A Harvard Apparatus Company Place Hamden Connecticut U S A Signature Je 7 dd He Full Name Ralph Abate Position Business Manager
10. ting the height of each reservoir within the holder The table below lists the approximate flow rates for a reservoir at the spec ified height with the supplied Teflon tubing Reservoir height Approximate flow rate 61 cm 24 in 14 ml min n n TE Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments Maintenance Cleaning Do not use alcohol aromatic hydrocarbons or chlorinated solvents for cleaning They may adversely react with the plastic materials used to man ufacture the system If salt solution spills on the valve assembly it should be cleaned as soon as possible with a soft cloth dampened with a mild solution of detergent and water NOTE Teflon Valves must be completely flushed with distilled water after each use Permanent damage will result if saline solution is allowed to crys tallize inside the valve The exterior of the CONTROLLER may be cleaned periodically to remove dust grease and other contamination It is not necessary to clean the inside Use a soft cloth dampened with a mild solution of detergent and water Avoid abrasive cleaners Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments Warranty The VC 8T Valve Control System is warranted to be free from defects in materials and workmanship for a period of two years from the date of ship ment If a failure occurs within this period we will repair or replace the fau
11. tivation of this circuit by an overflow condition immedi ately overrides all other settings and closes all valves Rear Panel The rear panel contains the power input module with fuse a 15 pin D con nector for the VALVE SET and 4 BNC connections for OVERFLOW IN STANDBY IN EXTERNAL ANALOG IN and EVENT OUT Valves A 15 pin D type female connector is used to connect the cable from the VALVE BRACKFT to the CONTROLLER Overflow In This BNC is used to connect the OVERFLOW SENSOR to the controller Place the sensor into position and plug it into this BNC Standby In This BNC is provided to allow the placing of the VALVE CONTROLLER into standby mode Standby mode disables EXT ANALOG IN inputs and closes all valves Publication 5715 001 REV A s Perfusion Valve Controller Model VC 8T ajud O 5 n td N O bei Control Description Conto Activation of this input is selected by placing the FRONT PANEL COM MAND input toggle into external analog mode and a TTL level high 3 to 5 V places the instrument in standby Ext Analog In This BNC is used to operate valves using analog voltage settings The volt age applied to this input selects the valve to open All other vales are closed Switching voltages closes the open valve and open the other Valve selec tions are overridden by the OVERFLOW SENSOR and the STANDBY BNC The table below indicates the selection scheme Applied Voltage V Valv
12. vid ed for all 8 channels of the VC 8 Controller Operation of the front panel TTL inputs is selected by placing the 2 position COMMAND INPUT TOGGLE into external digital mode Use of these inputs can allow the opening of more than one valve at the same time from a digital source such as your computer Placing the ON OFF TOGGLE SWITCH into the on position places the asso ciated valve into its open state A lit LED indicates the open state of the valve This setting is overridden by the overflow sensor Placing the ON OFFTOGGLE SWITCH into the off position places the valve into its closed state An unlit LED indicates the closed state of the valve Placing the ON OFF TOGGLE SWITCH into the ext position activates the associated BNC inputs A logic level low 0 V applied to the BNC places the associated valve into the closed state Correspondingly a logic level bigh 3 to 5 V places the associated valve into the open state A lit and an unlit LED indicates the open and closed states of the valve respectively These settings can be overridden by the overflow sensor Publication 5715 001 REV A Perfusion Valve Controller Model VC 8T Warner Instruments Control Description Conto Command Input Toggle The COMMAND INPUT TOGGLE is used to select between the front panel external digital controls and the rear panel external analog controls Overflow The OVERFLOW LED indicates an overflow condition sensed by the OVER FLOW SENSOR Ac
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