User`s Manual for the 3D Cell Explorer
Contents
1. 6 3 O 00 Software STEVE and how to operate the 3D Cell Explorer How to digitally stain cells with STEVE Choose a meaningful slice on the 2D visualization panel left side a Dragging the mouse up to down left click pushed on the 2D visualization panel or by moving the Slices slider Pick a new Stain a Click on the button Choose a name optional a Click on the black gray line edit form b Write down the name in the line text input Choose a color a Click on your desired color b Option drag the mouse to have a more precise vision of the color that you could select Go to the 2D visualization panel and draw a Click and or drag the mouse on your desired region of interest The pixels under your cursor are glowing in the panel view When you re lease the mouse we compute the stain from your selected pixels b Option You could zoom in on the 2D visualization panel by wheeling the mouse Now the stain is represented in the panel view and superimposed on the 2D visualization panel a Option you could change the weight of the colored image on the 2D visualization panel by moving the Overlay slider How to manipulate your stain a Change the opacity Opacity slider or drag up down with the right click pushed b Change the edge softness Edge softness slider or drag up down with the right click pushed on a stain edge c Move a stain in the panel view click on the stain and drag it on the desired position in the refractive2. Dimensions width x depth x height in mm 380 x 170 x 445 Weight in kg 8kg Storage and transport in packaging Use the provided dust cover when not in use Operation Permissible ambient temperature and can be operated between 15 C and 40 C Voltage ranges 100 240 VAC 50 60Hz 0 9 0 45A Camera CMOS 165 fps 1024x1024 pixels USB 3 Microscope Objective MO 60x magnification Air Laser wavelength 520nm Power output 0 1 mW Class I No eye protection needed Field of view FoV 94um Depth of field DoF 30 um Probing volume 80x80x30 um3 Lateral optical resolution 200nm Axial optical resolution 500nm Software Specification Cell Explorer mid 2015 Comments Lateral sampling 183 nm In native output Axial sampling 482 nm In native output Tomography frame rate acquisition 0 5 fps Full Acquisition of holograms Maximum speed of time lapse Use the provided dust cover when not in use Tomography frame rate reconstruction lt 1s Reconstruction of data based on GPU Self adjusting time lt 90 seconds Depending on optical thickness gt go back to contents 8 3 Start up It takes just moments to get up and running with the 3D Cell Explorer switch on the microscope position your sample and start the acquisition with our software Within seconds a full 3D image of your cell will be loaded to your screen and you can start exploring The microscope will then instantly self adjust So you can be sure you are getting the best
3. For each measurement you can visualize and modify the measurement properties called also data annotation On File menu the Properties menu open the annotation dialog Figure 14 All the visible properties marked with Eye icon will be added to the current measurement after the button Apply is pushed The invisible properties marked with Close Eye icon will not be saved The first property entries such as X size X resolution Acquisition Time Microscope etc are not editable you cannot modify them but they will always be saved on the measurement You can add new entries by clicking on the Add Entry button or you can delete the selected entry by clicking on Delete Entry By deleting an entry that entry will never be shown again on the annotation dialog you will have deleted it permanently Also you can edit each entry by clicking on the Edit icon button on the right side of the entry 6 Software STEVE and how to operate the 3D Cell Explorer P Acquisition time OF 27451903804 Microscope Cell Type Eukaryote Animal Fibroblast e T 7 Animal Age in hours Fibroblast Eo Figure 15 Data annotation 6 6 Howto update the software Steve will automatically update when a new version is available The user will be alerted to close Steve and the Steve Maintenance Tool will start In the Steve Maintenance Tool choose Update components and click the Next
4. button Follow the messages provided by the update dialog gt go back to contents 23 7 Troubleshooting and error messages If you experience trouble with your 3D Cell Explorer try the following solutions Consult your local Nanolive distributor or contact us at Support nanolive ch 7 1 Calibration Calibration failed err 1 1 If applicable reduce the quantity of liquid above your sample gt check Sample Preparation Protocol_Nanolive pdf in annex 1 Cell sample should not be thicker than 30 um Calibration failed err 1 2 If applicable check the transparency of your sample The 3D Cell Explorer requires samples to be transparent You might get this error in case your Sample absorbs or scatters too much light or if there are dirty surfaces in your sample preparation that are dimming the light sample gt check Sample Preparation Protocol_Nanolive pdf in annex 1 for correct sample preparation Calibration failed err 1 3 If applicable please clean the outer surfaces of your sample The 3D Cell Explorer is sensible to objects lying on the trajectory of the laser even if it is outside of the focus of the sample Such objects might be simply dust or finger prints which can be cleaned gt check Sample Preparation Proto col_Nanolive pdf in annex 1 for correct sample preparation In the case of living cell imaging in a dish it might also be dead cells that are floating on top of the liquid Try to wash your sample and
5. if using a top stage incubator tune it to minimize cell dying Calibration failed err 1 4 Sample may be too absorbent Try to move in a different area and re do the acquisition If the error persists please get in touch with our customer service 7 2 Acquisition Acquisition failure err 2 1 If applicable try to move to another position within your sample If the error persists try to clean your sample more gt check Sample Prepara tion Protocol_Nanolive pdf in annex 1 for information on cleanness of sample Acquisition warning err 2 2 To improve the acquisition quality try to clean your sample more Opaque objects within the field of view such as for instance nanoparticles might also cause this warning in which case it can be ignored gt check Sample Preparation Protocol_Nanolive pdf in annex 1 for information on clean ness of sample AD har EA FrAREAR be Aji gt go Dac k to contents 24 7 Troubleshooting and error messages 7 3 Connection Connection failure err 3 1 If the 3D Cell Explorer could not be connected to the computer please check that all cables are correctly plugged in and that the microscope has electrical power If the error persists unplug all cables reboot your computer and plug the cables back gt check section 3 for start up Connection failure err 3 2 At its first use Steve requires the computer to have access to the internet in order to retrieve specific calibration data matchin
6. index index gradient space d Change the shape of the stain select and edge or a corner and drag it To add other stains repeat the above steps Stain representation opacity edge softness stain edge rectangle panel view axes Stain s options a Enable Disable the visualization of the stain b Delete the stain c Change the color by double clicking the colored button or by clicking on the palette icon gt go back to contents 21 6 Software STEVE and how to operate the 3D Cell Explorer 6 4 Howto save your data The data can be saved by clicking on the Save icon which allows to save a 3D or a 4D vol file readable only with STEVE Alternatively it is possible to export the data in other file formats by opening the export dialog shown in the Figure 13 Dimensions Type Format R 2D Index i 6 J Vol Vo1x 3D O Stained O 4D wee Q Tif Png Jpeg Save the current 3D frame Save the refraction index Save 12 512 96 voxels data on Floats raw format Gae every 1 Figure 14 Export data setting The data can be exported by choosing e the dimension 2D current 2D image in the left panel 3D current 3D frame or 4D all the time lapse or the range between the markers A and B e the type index the refractive index or stained e the format of the file Raw Vol Vo1x Tiff Obj Png Jpeg available depending on the above selections 6 5 How to work with data annotation
7. the Export dialo Set playback speed Take a screenshot P ae lalog play p S6RBEasses Currently unavailable Viewer mode G 4 a Standard mode Switch between viewers Add new stain Delete stain Separate 3D view window Show stain Stain s information ea CO Live video recording Hide stain Change background color yACK FO ACK LU L CAnranrce CONCLCeEI IE 5 6 Software STEVE and how to operate the 3D Cell Explorer 2D View Panel Viewer 3D View The 2D view shows a X Y slice of the sample s Refractive The Panel Viewer represents a 2D space of Refractive The 3D view shows the stained data in three dimensions Index overlaid with the user defined stains Index and Index Gradient B Microscope File Stain 2D 3D Mode Help Debugger DyingCell vo1x 9 C A mM 10um Microscope Sliders Area Slices 48 96 Edge Softness s C Stain Coloring DyingCell_Stains xml Open amp Save Intermediar Vesicules Nucleus Buttons Area Sai O Q G Rescale eko oo ono ff n 002 00 00 11 67 A be B Transport Control Stain Picker 3D Control Area Stain Coloring Area Explanation of icons Time Line Area gt go back to contents 18 6 Software STEVE and how to operate the 3D Cell Explorer In order to start your measurements verify that all the cables are properly plugged in the 3D Cell Explorer is turned on and connected to your computer and S
8. to the customer Do not disassemble any parts of the microscope as this affects the function or reduces the performance of the microscope Please be aware that the warranty expires if you remove the covers Keep the instrument clean and do not contaminate the optical ele ments when wiping away the dust on the instrument Contaminations on the objective and the mirrors like fingerprints and oil smudges could be gently wiped with the provided cleaning swab and a bit of alcohol Ethanol or Isopropanol Note that alcohol is highly inflammable do keep it away from fire or potential sources of electrical sparks and use it in a well ventilated room More infor mation on the cleaning procedure to be found in section Cleaning Procedure for Optics of the 3D Cell Explorer Do not attempt to use organic solvents to clean the microscope To clean it use a lint free soft cloth slightly moistened with clear water During use if the microscope is splashed by liquid cut off the power at once and wipe away the splash Place the instrument in a cool dry position When not using the mi croscope or carrying it keep it covered with the provided dust cover Contents 1 oe a Introduction 1 1 TZ 1 3 1 4 1 4 Company Introduction The 3D Cell Explorer instrument description and main features Typical fields of application potential applications 3D Cell Explorer Terminology 3D Cell Explorer Terminology Technical Specificat
9. NINOLIVE Looking inside life User s Manual for the 3D Cell Explorer Version 1 2 July 2015 Sabine Bautz Thank you for purchasing the Nanolive 3D Cell Explorer You are now ready to start exploring living cells Before using your 3D Cell Explorer carefully read the safety notes to ensure safe handling and usage of the device Please refer to the Quick Start Guide to learn how to remove the packaging safely from the 3D Cell Explorer Safety Notes Ty 2 Carefully open the box with the top face up to avoid damaging the 3D Cell Explorer Handle the 3D Cell Explorer from the side or the bottom plate un plug all cables before moving it When moving the microscope carefully carry it without lifting it from the camera and avoid any shocks Do keep the instrument out of direct sunlight high temperature or humidity dusty and easy shaking environment Make sure the table surface is flat horizontal and firm Verify that the computer is plugged and grounded earthed with its own power supply during usage with your 3D Cell Explorer Supply the 3D Cell Explorer only with the provided power supply No special laser safety required Class I laser Maintenance and Care 1 All elements e g microscope objective mirrors lenses etc have been specially and carefully adjusted please do not dismount repo sition or modify them In such case the warranty becomes automat ically void and rework shall be charged
10. TEVE is running Operate and control the 3D Cell Explorer easily with STEVE if you wish to do the following e Fully automated microscope calibration e Single frame image acquisition single 3D reconstruction put icon 3D of the single shot e Time lapse acquisition multiple 3D reconstructions in a range of time put icon 4D for the time lapse e Sample screening using auxiliary bright field mode white light 6 2 1 Fully automated microscope calibration With the 3D Cell Explorer there is no need for sample preparation or manual calibration After positioning your sample the microscope will in Stantly self adjust so you can be sure you are getting the best possible images of your cells You can always check the calibration progress as it is displayed on STEVE After calibration is completed the cell image is displayed on the left panel of STEVE 6 2 2 Step by step guide on how to prepare for a measurement 1 Take the sample previously prepared for details on how to prepare the sample please refer to the sample preparation protocol and place it on the 3D Cell Explorer stage 2 Open STEVE and click on the white light button On the left panel of STEVE you can visualize the white light mode Use the XY and Z knobs of the 3D Cell Explorer to search for the cell of interest and focus on it 3 Now you can choose to either do a single frame image acquisition or a time lapse acquisition 6 2 3 How to do a single frame image
11. acquisition A single frame image acquisition is an instantaneous measurement that results in a unique image of the object To perform a single shot click on the 3D button or on the Single Shot from the Microscope menu At the end of the acquisition the software STEVE will enable to digitally stain the data and interactively explore the cell and its subcomponents Further down we explain the exact steps on how to achieve this Chapter How to digitally stain cells with STEVE gt go back to contents 19 6 Software STEVE and how to operate the 3D Cell Explorer 6 2 4 How to do a time lapse acquisition To perform a time lapse click on the 4D button or on Time Lapse in the Microscope menu Furthermore the speed and the duration of the acquisition needs to be set Figure 12 Figure 13 time lapse setting window The time lapse can be performed either at the maximum speed or by setting the time interval between two consecutive frames The duration of the time lapse acquisition can be determined either by stopping it manually by clicking on the Stop Acquiring button or by setting the ending time for it to stop automatically At the end of the acquisition the software STEVE will enable to digitally stain the data and interactively explore the cell and its subcomponents Further down we explain the exact steps on how to achieve this Chapter How to digitally stain cells with STEVE gt go back to contents 20 6
12. and Surface View 4 Detach the 3D view From STEVE s main window I Take a screenshot of the 3D view 4 Start or stop a live video recording of the 3D view BUTTONS AREA Q Visualize your sample in white light Move the microscope s knobs to search and Focus your cell of interest 3D Single shot acquisition acquire a single 3D Frame 4D Time lapse acquisition acquire multiple 3D Frames in a range of time Fy Load a measurement file vol fd Save a 3D or 4D measurement File vol To save a 4D file please indicate the desired range on the time line using markers and I Open the Export dialog SLIDERS AREA Overlay Fuzzy switch between stained data and unaltered Refractive Index Slices Selects the X Y slice from the 3D data stack Opacity Chooses the opacity for the currently selected stain Edge Softness Use this to make a stain s edges soft and avoid solarized images in 2D STAIN COLORING AREA PANEL Load Panel Load a previously saved panel xml format Save Panel Save the current panel stains Rescale Modify the viewing range of the panel viewer to Fit the current time Frame Software STEVE and how to operate the 3D Cell Explorer STAIN PICKER New stain choose a name not required and a color For the stain Next begin painting your region of interest in the 2D view A rectangular stain is computed based on the painted part of the image and is shown in the panel vie
13. d Frame the 3D Cell Explorer measures the Refractive Index of your cellin three dimensions Use STEVE to digitally stain your data and interactively explore your cell and its subcomponents 2D VIEW The 2D view shows an X Y slice of the sample s Refractive Index overlaid with the user defined Stains In this view you can Move to a different slice or via Slices slider Zoom in or out Move the view when zoomed in SHIFT rrr Measure or stop measuring a user defined path length Click this button and click on the image to draw a path Show the Refractive Index and the Index Gradient in the Panel Viewer of a selected voxel in the 2DView fe Take a screenshot of the 2D view W 4 Switch between Standard mode and Viewer mode PANEL VIEWER The panel viewer represents a 2D space of Refractive Index horizontal axis and Index Gradient vertical axis Stains are shown as rectangles covering a region in the staining space Regions on the right have a high Refractive Index regions on the top have a high Index Gradient In this view you can Change a stain s position on the stain s center or shape on the Stain s edge Change a stain s transparency on the stain s center edge or via the Opacity and Edge Softness sliders 3D VIEW The 3D view shows the stained data in three dimensions In this view you can rotate Zoom f Move SHIFT the 3D data 3 Switch between Voxel View
14. eparation Improved image resolution is achieved by employing a synthetic aperture and multiple viewpoint holographic methods After the holograms have been captured high resolution images of each plane in the sample are created by computer processing see Cotte et al Nature Photonics 7 2013 113 17 1 1 2 1 3 Introduction The 3D Cell Explorer instrument description and main features Class I laser source 3D image reconstruction in probing volume Operation modes 3D snap shot single measurement time lapse measurement Full and automatic self alignment of the microscope Non invasive technology for probing living in vitro cells Measuring cellular processes with real time kinetics enables multi parameter analysis at single cell and sub cellular scale Typical fields of application potential applications The 3D Cell Explorer is a tool of discovery for cell researchers and biologists in a limitless number of fields including Cell division Cell morphology monitoring Visualize and monitor microorganism interaction and internalization Cell differentiation Cell cell interaction Intracellular trafficking Cellular remodelling processes Cell death apoptosis or necrosis Drug monitoring i e monitor cell response to treatments in term of modification in the cell s morphology as well as if it is sensible or not to drugs In vitro fertilization Observe the consequence on cell morphology upon exposure to different t
15. erate the 3D Cell Explorer 6 1 Short introduction of STEVE STEVE is Nanolive s software for exploring the data acquired using the 3D Cell Explorer For each recorded frame the 3D Cell Explorer measures the refractive index RI of your cell in three dimensions Use STEVE to digitally stain your data and interactively explore your cells and its subcomponents Software features Simple and advance microscope control Single Shot acquisition Configurable time lapse acquisition Object selection using auxiliary bright field mode Full self adjustment Intuitive user interface Quantitative staining based on physical markers RI Easy management of your digital stains creation edit enable disable delete save Playback options for time lapse data Comprehensive visualization options 2 visualization modes Control mode and Viewer mode 2D slice per slice viewer refractive index and stained data combined 3D experience scientific viewers Multiple data export options Classical Formats raw tiff obj png jpeg NANOLIVe Compressed proprietary formats vol vo1x SSS Screenshot capture for 2D and 3D viewers Video capture of the 3D viewer avi Data annotation system Semi automatic update of the software GPU accelerated 3D processing iL O Contents CONLE ILCD 6 Explanation of icons Getting started with STEVE STEVE is Nanolive s software for exploring the data acquired using the 3D Cell Explorer For each recorde
16. g your device If the calibration data could not be downloaded please check if your local network has internet connection If the error persists ask your network admin istrator If the computer cannot be connected to the internet please contact us in order to send you the calibration data file 7 4 STEVE Acquisition interrupted err 4 1 Acquisition with Steve requires at least 500MB of free disk space Please free up space on your primary hard disk drive File saving failure err 4 2 Please ensure you have enough space on your hard drive and that you have writing permission in the folder you wish to save your data If the error persists contact your computer s administrator gt go back to contents 25
17. ically by a swiping movement so that the contaminant is removed from the surface of the optical element as soon as possible avoid dragging it around Optical surfaces are sensitive to scratches and must thus be cleaned carefully with the appropriate equipment We advise to use only new clean swabs Texwipe Microdenier or Alpha series soaked with ethanol or iso propanol to clean the optics fe Take a fresh and clean swab soaked in alcohol and without pressure swipe the contaminant away from the center of the optical element Use a fresh side of the swab after each contact with the optical surface in order Since as to avoid re deposition of removed contaminants Please clean first the most exposed optical surfaces with thus higher probability of contamination e The MO is quite exposed to contamination and it may thus be required to clean it regularly e The scanning mirror of the rotating arm is not much exposed and its cleaning should not be required too often e Finally the central mirror is not exposed and its cleaning should be exceptional High concentrations of contaminants may require repeated cleaning Figure 10 Cleaning procedure for microscope objective gt go back to contents 14 5 Cleaning Procedure for the optical elements Figure 11 Cleaning procedure for scanning mirror Figure 12 Cleaning procedure for central mirror gt go back to contents 15 6 Software STEVE and how to op
18. ion Sheet TSS Start up Sample Preparation Protocol Cleaning Procedure for the optical elements Software STEVE and how to operate the 3D Cell Explorer 6 1 6 2 1 6 2 2 6 2 3 6 2 4 6 3 6 4 6 5 6 6 Short introduction of STEVE Fully automated microscope calibration Step by step guide on how to prepare for a measurement How to do a single frame image acquisition How to do a time lapse acquisition How to digitally stain cells with STEVE How to save your data How to work with data annotation How to update the software Troubleshooting and error messages 7 1 1 2 7 3 74 Calibration Acquisition Connection STEVE 1 Introduction 1 1 Company Introduction Nanolive SA was incorporated in November 2013 by Dr Yann Cotte Dr Sebastien Equis Prof Dr Christian Depeursinge Dr Fatih Toy and Dr Andreas Kern and has developed a disruptive world exclusive technology which allows for the first time to discover a living cell from the inside in 3D and in color This is done without the use of any labeling With existing methodologies it is today impossible to see inside a living cell without damaging or modifying it Nanolive s technology allows doing so with a much higher resolution than normally available With Nanolive s microscope the 3D Cell Explorer researchers students and medical doctors will directly experience in real time what happens inside a living cell 1 2 The 3D Cell Explorer instrument descripti
19. on and main features Our tomographic holographic 3D microscope is the result of years of work on development By a combination of holography and rotational scan ning the system detects changes to light as it propagates through the cell The sample is positioned between a high numerical aperture air objec tive beneath the sample and a rotational illumination arm above This optical path forms one arm of a Mach Zehnder interferometer set up with the other being the reference path Green light for laser wavelength specification please check TSS below from a diode laser is split into sample and reference beams the sample beam illuminates the sample through the rotational illumination arm at a very steep angle A hologram is recorded on a digital camera by combin ing the beam that has passed through the sample with the reference beam The sample beam is then rotated by a small angle and the process is repeated with one hologram recorded for each beam position The parameter measured by the 3D Cell Explorer is neither absorption nor fluorescence intensity of an exogenous molecule as with most light op tical microscopes Instead the physical refractive index of the sample is obtained in a three dimensional 3D distribution with a resolution better than the diffraction limit given by the microscope objective The output is the refractive index distribution within the cell The result is quantitative cell tomography in vitro without any invasive sample pr
20. possible images of your cells This packaging contains the following 1 the 3D Cell Explorer 2 a USB 2 0 cable 3 a USB 3 0 cable 4 a power supply and 5 a cleaning set with cleaning swabs Figure 3 Contents of the 3D Cell Explorer packaging gt go back to contents 9 3 Start up e Remove all components from the packaging following the instructions in the quick start guide Never lift the 3D Cell Explorer by holding the camera GO Holding spots Figure 4 Holding spots for the 3D Cell Explorer e Place the base on a low vibration flat worktable e Remove the re useable dust cover and foils re useable dust cover can be used for storage purposes as well gt go back to contents 10 3 Start up Re usable dust cover ae ae 2 Protection Foils eX Ast Fe i 7 Figure 5 Dust cover and protection foils on the accessible optical elements Properly dispose of original packaging or keep it for storage or return of the instrument to the manufacturer Now you are ready to install the 3D Cell Explorer e Make sure the power switch is off e Plug in the power supply into the power port e Plug in the USB 3 0 cable into the USB 3 0 port on the side of the 3D Cell Explorer tightly screw the locks and connect it to the USB 3 0 slot on your computer e Plug in the USB 2 0 cable into the USB 2 0 por
21. t on the back of the 3D Cell Explorer and connect it to the USB 2 0 slot on your computer gt go back to contents 11 3 Start up Plug port USB 3 0 on the side of your 3D Cell Explorer 3D Cell Explorer Nanolive SA SN YY MM DD 00 00 XX XX XX Figure 6 Connecting plugs of the 3D Cell Explorer Use only the power supply and the USB cables provided with the system Contact Nanolive in case of malfunction Now you are ready to download the software STEVE e Switch on your PC and make sure it is connected to a power source e Go to www nanolive ch register and register yourself and your 3D Cell Explorer e When requested insert the serial number You can find your serial number on the backside of your 3D Cell Explorer see Figure 6 bottom right e Download and install STEVE e Start STEVE while still connected to the internet e Switch on your 3D Cell Explorer and make sure it is connected to a power source e Before starting exploring your cells check our guide on how to properly prepare your samples gt go back to contents 12 4 Sample Preparation Protocol Please see the attached Sample Preparation Protocol_Nanolive pdf in annex 1 NB The latest version can also be found online http nanolive ch applications protocols 5 Cleaning Procedure for the optical elements First inspect the optical elements to determine the location of the con taminants This allows you to anticipate the cleaning typ
22. wer D Hide or show a stain fi Delete a stain e Show the stain properties and allow background definition 3D CONTROL AREA Change the background color for the 3D view Hide Axes amp Cube Show hide the coordinate axes and the outline cube in the 3D view Show Caption Show hide the labels in the 3D view Cropping sphere Cut away part of the 3D view Move the plane through the sphere to crop the 3D data The plane can be moved SHIFT amp or rotated TRANSPORT CONTROL Use this to explore your data in time The transport display shows the frame number and acquisition time of the current Frame I lt Jump to the beginning of a data set Set marker A to the current cursor position gt Play pause playback Set marker B to the current cursor position Jump to the end of a data set Set playback speed Note Markers A and B select a time range for saving exporting purposes TIME LINE AREA v AB Displays the current time Frame and the selected range Click on the top part of the area to jump to the specified frame Drag on the tick region to set markers A and B Left click drag White Light mode Current time Frame Right click drag Single shot acquisition Set marker A or B Mouse wheel Time lapse acquisition Jump to the beginning Load a vol file Play pause playback Measure i J to th d Show Refractiv Index value Save a 3D or 4D vol file ump to tne en Open
23. ypes of nanoparticles as well as test the behaviour of them and monitor their location Tissues imaging histopathological studies tissue morphological studies etc For further information please visit our web page http nanolive ch applications gt go back to contents ion 1 Introduction 1 4 3D Cell Explorer Terminology eS a UT mmm LA r ms Front View Side View Rear View Figure 1 views of the 3D Cell Explorer gt go back to contents 6 1 Introduction 1 4 3D Cell Explorer Terminology Peripheral mirror oc Scanning head Cell container interface 9 0 0 0 XY knob for sample screening Camera ase Figure 2 terminology of key elements in the 3D Cell Explorer Microscope objective Central mirror XY stage For sample screening Z knob for optical Focusing gt go back to contents 7 2 Technical Specification Sheet TSS Hardware Specification Cell Explorer mid 2015 Comments
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