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1. 0 lt zu Z 0 m U C lt U 0 n D Q ct O 2 c Q SYRINGE PUMPS Choosing the Right Pump for Your Application amp Budget SYRINGE PUMP QUESTIONS What is your application How many syringes will be used simultaneously What size syringe will be used What flow rate s will be used See pump reference pages 102 106 What is the total volume to be delivered Does the pump need to withdraw fill the syringe as well as infuse dispense Selection Guide INFUSION ONLY MODEL PUMP 11 ELITE PUMP 22 PHD 22 2000 PHD ULTRA PUMP 11 ELITE PICO PLUS ELITE STANDARD PUMP are PC1 55 2222 PC1 70 2000 PC1 70 3005 PROGRAMMABLE PUMP 2 5 eon PC1 70 4506 NUMBER OF SYRINGES l r 2 0 10 209105 Zio kG 1 or 2 2 MINIMUM SYRINGE SIZE 0 5 ul 0 5 ul 0 5 ul 0 5 ul 0 5 ul 0 5 ul MAXIMUM SYRINGE SIZE 20 60 ml single 140 ml 140 ml 140 ml 50 60 ml single 10 ml 10 ml dual 10 ml dual MINIMUM FLOW RATE 1 28 pl min 0 002 ul hr 0 0001 ul hr 1 56 pl min 1 28 pl min 0 54 pl min MAXIMUM FLOW RATE eee ssi mmn 220 82 ml min bend 170 ml min AVERAGE LINEAR FORCE COMPUTER CONTROL 1 0 TTL CONNECTION DIMENSIONS H x W xD PUMP HEAD DIMENSIONS H x W x D WEIGHT PAGE 25 99 ml min dual 16 kg 35 Ibs adjustable USB No 226x1778 l5 Cm 9 x x6 in 2 1 kg 4 61 Ibs li 25 99 ml min dual 23 kg 50 Ibs or 30 kg 66 Ibs 34 kg 75 Ibs adjustable 16 kg 35 Ibs adjus
2. Legendary reliability 2 year warranty BENEFITS Bulk Fluid Injections Fluid Sampling Drug Delivery Q Gradients Continuous Infusion The Harvard 33 Dual Syringe Pump is a breakthrough in pumping technology The 33 has two independent pumping channels linked through hardware and software When combined with a valve box page 42 it provides the continuous delivery of a peristaltic or piston pump with the accuracy absence of pulsation and low flow rates of a syringe pump Several modes of operation are available to accommodate a range of setups and experimental protocols A unique movable limit switch mechanism is used to change direction or stop operation of the pump depending on the mode of operation Reciprocal Parallel Mode Syringe mechanisms can run in the same or opposite directions i e both infusing or withdrawing at the same time or one infusing and the other withdrawing Proportional Mode Different flow rates and syringe diameters can be set for each syringe mechanism AutoStop Mode Pump stops operation when a limit switch Is activated Continuous Run Mode When a limit switch is activated each syringe mechanism reverses direction This pump has high pressure capability TTL and RS 232 interface for data acquisition and computer control The communication ports enable daisy chaining of up to 100 pumps Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 TYPE ACCURAC
3. o o 0 O O O Does the pump need to have TTL capabilities ex external control of valves use of footswitch etc INFUSION WITHDRAWAL PUSH PULL PRR MGT UN PUMP 33 PHD 22 2000 PHDULTRA PHD ULTRA HPS PHD ULTRA fiie PC1 55 3333 PC1 70 2001 PC1 70 3006 PC1 70 3008 PC1 70 4507 PC1 70 3601 PC1 70 2002 PC1 70 3007 PC1 70 3111 is er PC1 70 3009 1 1 2 2 to 10 2 to 10 4 1 oe e 0 5 ul 0 5 ul row 0 5 ul 0 5 ul 20 ml 2 5 ml OS Ul 1 ml 1 ml 140 ml 140 ml 140 ml 200 ml 100 ml 140 ml 3 66 pl min 3 66 pl min 0 0004 ul Rr 0 0001 ul hr 1 56 pl min 49 7 nl min 3 06 pl min 1 56 pl min 3 818 ml min 3 818 ml min 106 6 ml min 215 8 ml min 215 8 ml min 144 1 ml min 215 8 ml min 215 8 ml min 5 kg 11 Ibs 5 kg 11 Ibs 26 ke 57 Ibs 23 kg 50 Ibs 34 kg 75 Ibs 197 kg 433 Ibs 91 kg 200 Ibs 34 kg 75 Ibs adjustable adjustable or 30 kg 66 Ibs adjustable adjustable adjustable adjustable USB USB RS 232 RS 232 USB RS 232 USB RS 232 USB RS 232 USB RS 232 Yes nes Yes Yes Yes nes Yes nes 22 6x1 8x9 32 cm PT Fe 15 2x31 1x28 6cm ges wl 10 16x30 5x21 6 cm BE Me 30 5x21 6x10 8cm BOG sts POEM 9x 7x 3 67 in 12 0x8 5x4 38in 6x12 5x11 25 in 6 3x9x11 in 4x12x8 5 in 12x8 5x4 25 in 12x8 5x4 25 in 4x12x8 5 in 6 35x 5 08x 19 05 cm PETN B 22 9 x 43 2 x30 5 cm 2 40 64 x 30 5 x 19 7 cm 7 29502 0751 2 595 2 0507 51 9 x17x12 in 16x 12x 7 75 in 1 96 kg 4 32 Ibs 6 8 kg 15 Ibs 4 5 kg 10 Ibs 4 5 kg 10 Ibs 1
4. 4 cm 11 0 x 5 3 x 7 25 in 6 4 kg 14 1 Ib PC1 70 3110 fax 508 429 5732 High capacity up to 800 ml with four 200 ml syringes Ultra high pressure delivers over 400 Ibs of pumping force Program simple to complex Methods without a PC Built to last rugged construction for a lifetime of service Accepts only Harvard Apparatus stainless steel syringes Legendary reliability 2 year warranty Remote Microprocessor multiple syringe infusion withdrawal programmable 0 50 0 05 20 ml to 200 ml stainles steel only 50 70 nl min 144 1 ml min 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 197 kg 433 lbs 100 Force Selection 1 8 Stepper Motor 100 240 VAC 50 60 Hz 75 W 0 5 A fuse 30 48 x 21 59 x 10 8 cm 12 x 8 5 x 4 25 in 40 64 x 30 48 x 19 69 cm 16 x 12x 7 75 in 13 7 kg 30 2 Ib PC1 70 3111 www harvardapparatus com SYRINGE PUMPS NEW PHD ULTRA Nanomite Stereotaxic Mounted Injector HARVARD Control Unit CC KEY FEATURES Q Light weight makes it ideal for hand held or stereotaxic injection Easy to use LCD color touch screen with GUI interface Q Create and store gt 50 programs for High performance in a small package Q 1000 times better than manual syringes APPLICATIONS Cellular Injections Drug Delivery Microinjections Hand Held Automated Delivery Stereotaxic Injections Regenerative Medicine The PHD ULTRA Nanomite i
5. 5 619 630 Utada A S et al Monodisperse Double Emulsions Generated from a Microcapillary Device Science 2005 Vol 308 537 Liu Tsang V etal Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels FASEB Journal 2007 Vol 21 790 801 fax 508 429 5732 www harvardapparatus com 29 N lt a Z O m U C lt U o TU I 0 N M N o o O gt Q lt O I Q D Q lt i va D 7 c 3 U o 0 lt a Z C I U C lt U 0 U I U N N N O O Q lt QD I Q D Q 0 lt zi I o O 7 c j o 0 30 SYRINGE PUMPS PHD 22 2000 Advanced Syringe Pumps continued PHD 22 2000 Syringe Pumps SYRINGE PUMP VERSIONS Standard Force High Force Standard Force High Force Standard Force High Force Standard Syringe Pumps STANDARD 2 SYRINGE PC1 70 2000 PC1 71 2000 PC1 70 2001 PC1 71 2001 PC1 70 2002 PC1 71 2002 6 10 MULTI RACK PC1 70 2003 PC1 71 2003 PC1 70 2006 PC1 71 2006 PC1 70 2009 PC1 71 2009 4 X 140 MULTI RACK PC1 70 2004 PC1 71 2004 PC1 70 2007 PC1 71 2007 PC1 70 2010 PC1 71 2010 MICROLITER RACK PC1 70 2005 PC1 71 2005 PC1 70 2008 PC1 71 2008 PC1 70 2011 PC1 71 2011 Remote Syringe Pumps STANDARD 2 SYRINGE PC1 70 2100 PC1 71 2100 PC1 70 2101 PC1 71 2101 PC1 70 2102 PC1 71 2102 6 10 MULTI RACK PC1 70 2103 PC1 71 2103 PC1 70 2106 PC1 71 2106 PC1 70 2109 PC1 71 2109 4 X 140 MULTI RACK PC1 70 2104 PC1 71 2104 PC1 70 2107 PC1 71 2107 PC1 7
6. C lt T C O m lt O I Q a U D Q D 0 lt 2 va D 7 c 3 U SYRINGE PUMPS Customized Solutions OEM and Special Syringe Pumps continued OEM Modules Microliter Syringe Pump Module OEM Modules Milliliter Syringe Pump Module KEY FEATURES Accuracy of 0 5 Q Footprint of 4 5 x 9 25 x 3 25 inches Q 0 001 yl to 1 33 ml min Q WEEE EU RoHS KEY FEATURES Accuracy of 0 5 Q Footprint of 5 3 x 9 5 x 4 25 inches Q 0 001 yl hr to 44 28 ml min Q WEEE EU RoHS Specifications Specifications TYPE ACCURACY REPRODUCIBILITY SYRINGE TYPE SYRINGE SIZE Size Minimum Size Maximum FLOW RATE Minimum Maximum AVERAGE LINEAR FORCE CONNECTORS RS 232 DC Power POWER DIMENSIONS Overall Hx Wx D Mounting WEIGHT Order PC1 72 2220 PC1 72 2225 Harvard Apparatus phone 508 893 8999 toll free US 800 272 2775 Product Microprocessor single syringe infusion withdrawal 0 5 0 1 Glass or plastic 0 5 ul 1 ml 0 001 ul hr 1 33 ml min 2 73 kg 6 Ibs A pin RJ 11 telephone jack dual RS 232 ports 4 pin Header Friction lock 12VDC 5 1A 12W User supplied 11 4 x 23 5 x 8 3 cm 4 5 x 9 25 x 3 25 x 4 5 in 22 2 x 7 0 cm 8 75 x 2 75 in Mounting holes for 4 8 screws 0 84 kg 1 85 Ibs Microliter Syringe Pump Module without Power Supply Microliter Syringe Pump Module with Power Supply TYPE ACCURACY REPRODUCIBILITY SYRINGE TYPE SYRINGE SIZ
7. RATE Minimum Maximum DISPLAY CONNECTORS RS 232 RS 485 USB 1 0 amp TTL Footswitch AVERAGE LINEAR FORCE STEP RESOLUTION VOLTAGE RANGE DIMENSIONS H x W x D Control Box Injector Unit WEIGHT Control Box Injector Unit 00 Q 9 00 00 00 36 0O ul 100 Specifications Microprocessor single syringe Infusion Withdrawal Programmable 0 5 0 05 Glass or plastic 0 5 ul 1 ml 3 66 pl min 3 818 ml min 4 3 WQVGA TFT color display with touchscreen 9 pin D Sub connector IEEE 1394 6 position Type B 15 pin D Sub connector Mini phono jack 5 kg 11 lbs 100 force selection 0 198 um ustep 100 240 VAC 50 60 Hz 75 W 0 5 A fuse 30 5x21 6x 11 1 cm 12 0 x 8 5 x 4 38 in 6 35 x 5 08 x 19 05 cm 2 5 x 2 0 x 7 5 in 2 06 kg 4 55 Ibs 0 458 kg 1 01 Ibs REGULATORY CERTIFICATIONS CE EU RoHS Order PC1 70 3601 Product PHD ULTRA Nanomite Infusion Withdrawal Programmable Single Syringe fax 508 429 5732 www harvardapparatus com 23 SUWOURN VAAN GHd SdWNe 0 lt a PA C m U C lt U 0 Zz O 2 Oo Q Q 2j 3 D O ct O SYRINGE PUMPS NEW NanoCool Injector ie NanoCool Hand held or Stereotaxic Mounted Injector Da Volumes Omer gt GO 4 HARVARD CC Control Unit Footswitch KEY FEATURES by Organ Cell Type 18 C for optimal cell viability on any delivery syringe Oxygen Change in 0 and eppendorf
8. a PC The Pump 11 Pico Plus Elite is a dual syringe Infusion Withdrawal Programmable Model It has a footswitch input USB serial port for computer control RS 485 ports for daisy chaining pumps and Digital I O for external control via an independent computer or device see page 13 for more information on connectivity There is also an option of daisy chaining pumps through the RS 232 RJ 11 ports For more information on the Pump 11 Pico Plus Elite including features and programming see the Pump 11 Elite on page 11 Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 Specifications TYPE ACCURACY REPRODUCIBILITY SYRINGE Type Size Minimum Size Maximum FLOW RATE Minimum Maximum DISPLAY CONNECTORS RS 485 USB 1 0 amp TTL Footswitch AVERAGE LINEAR FORCE STEP RESOLUTION INPUT POWER INPUT POWER CONNECTION POWER SUPPLY DIMENSIONS H x Wx D WEIGHT REGULATORY CERTIFICATIONS Order Product PC1 70 4506 Microprocessor dual syringe infusion withdrawal programmable 0 35 0 05 Plastic or glass 0 5 ul 10 ml 0 54 pl min 11 70 ml min 4 3 WQVGA TFT color display with touch screen IEEE 1394 6 position Type B 15 pin D Sub connector Mini phono jack 16 kg 35 Ibs 100 Force Selection 0 031 um ustep 12 30 VDC 2 5mm ID x 5 5 mm OD male plug 100 240 VAC 50 60 Hz 8 Watts Universal Power Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 226xl
9. com SYRINGE PUMPS NEW PHD ULTRA Advanced Syringe Pumps continued Alarms There are several alarms available on the PHD ULTRA Syringe Pumps End of Run Near End of Run Power Up Stall Calibration Reminder You may chose to activate one activate them all or mute them all Advanced GLP Documentation Features e Download experimental parameters to PC e Alpha Numeric capability PHD ULTRA Satellite Pumps PHD ULTRA Satellite Pumps The NEW PHD ULTRA Satellite Pumps are infusion withdrawal pumps They are available in three configurations standard push pull and high force The Satellite Pumps are combined with stand alone PHD ULTRA Pumps to create a Gradient System Satellite Pumps can only be powered from a stand alone PHD ULTRA via RS 485 They cannot be controlled with a PC Satellite Pumps include a footswitch input USB and RS 485 connectors See page 20 for part numbers PHD ULTRA Remote Pumps Upgrade We offer pumps that can be upgraded If you buy an infusion withdrawal pump and later decide you want programmability you can upgrade it You pay a lot less than buying a whole new pump Accessories A full range of accessories are compatible with the PHD ULTRA including syringe heaters in line heaters and coolers microfluidic circuits connectors tubing syringes and more PHD ULTRA Syringe Pump Modules CC PHD ULTRA Syringe Pump Modules The NEW PHD ULTRA Syringe Pump Modules a
10. conductor VOLTAGE RANGE Universal input 100 240 VAC 50 60 Hz TTL 9 pin D sub connector CABLE LENGTH 152 cm 5 ft approx AVERAGE LINEAR FORCE 91 kg 2200 Ibs DIMENSIONS H x W x D STEP RESOLUTION 0 082 um step Control Box 9 5 x 27 9 x 22 9 cm 3 75 x 11 x9 in VOLTAGE RANGE Universal input 100 240 VAC 50 60 Hz Syringe Holder 22 9 x 43 2 x 30 5 cm 9 x 17 x 12 in DIMENSIONS HxWxD 17 x 23x 29 cm 6 7 x9 0x 11 4 in WEIGHT 6 4 kg 14 Ibs Order Product REMOTE CABLE 9 1 m 30 ft Length PC1 70 2023 PHD 22 2000 Hpsi Programmable Syringe Pump Plastic and glass syringes are not recommended because of high force PC1 72 0199 Remote Extension Cable 1 5 m 5 ft PC1 72 1405 Remote Extension Cable 9 1 m 30 ft MA a Hide PC1 70 2022 RS 232 D Sub 9 Pin to RJ11 4 Connection Cable PC1 70 2200 PHD 4400 Hpsi Programmable Single Syringe Pump PC1 70 2201 PHD 4400 Hpsi Remote Programmable Single Syringe Pump Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 31 0 lt a PA m U C lt U 0 U I U N E N o o O I U e O 2 Q U I U A A o O I U 2 T ya 2 S c j D Ro U 3 M o o c 3 D y A O ya 3 O 3 3 O D o lt 2 va D y c 3 U o iU 24 e Zz m U C S U o lt TI A k O o lt va D y am U SYRINGE PUMPS NEW MFRP10 Syringe Pump KEY FEATURES Occlusion alarm Automatic sy
11. information on the Pump 11 Elite Nanomite including features and programming see the Pump 11 Elite on page 11 Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 TYPE ACCURACY REPRODUCIBILITY SYRINGES MIN MAX FLOW RATE Minimum Maximum DISPLAY CONNECTORS RS 485 USB 1 0 amp TTL Footswitch AVERAGE LINEAR FORCE STEP RESOLUTION INPUT POWER INPUT POWER CONNECTION POWER SUPPLY DIMENSIONS H x W x D Control Box Mechanism WEIGHT Specifications Microprocessor single syringe infusion withdrawal programmable 0 5 0 05 0 5 ul 1 ml 3 66 pl min 3 818 ml min 4 3 WQVGA TFT color display with touch screen IEEE 1394 6 position Type B 15 pin D Sub connector mini phono jack 5 kg 11 lbs 100 force selection 0 198 um ustep 12 30 VDC 2 5 mm ID x 5 5 mm OD male plug 100 240 VAC 50 60 Hz 8 Watts Universal Power Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 22 6 x 17 78 x 9 32 cm 9 x 7 x 3 67 in 2 5 x 2 0 x 7 5 in 6 35 x 5 08 x 19 05 cm 1 96 kg 4 32 Ibs REGULATORY CERTIFICATIONS CE EU RoHS Order PC1 70 4507 Product Pump 11 Elite Nanomite Infusion Withdrawal Programmable Single Syringe fax 508 429 5732 www harvardapparatus com 15 N lt I Z a m T C lt y T y C 3 U m ct D Z O I O 3 ct D 6 lt 7 Z m U C lt U 6 U L U C F AJ gt gt Q lt Q O i6 Q o l
12. special transformer designed for minimum RFI Radio Frequency Interference This new transformer cuts electrical noise so that it is almost non existent With the PHYSIO 22 even the most sensitive sensors will not show a noise spike Specifications TYPE ACCURACY REPRODUCIBILITY SYRINGE Type Size Minimum Size Maximum FLOW RATE Minimum Maximum CONNECTORS RS 232 TTL AVERAGE LINEAR FORCE STEP RESOLUTION VOLTAGE RANGE DIMENSIONS Hx WxD Microprocessor multiple syringe infusion only or infusion withdrawal 0 35 0 05 Plastic glass or stainless steel 0 5 ul 140 ml 0 002 ul hr 55 1 ml min 25 pin connector Shared port with RS 232 21 36 47 Ibs 0 33 um step 95 to 130 VAC 60 Hz 220 to 260 VAC 50 Hz selectable 28 x 22 2 x 14cm 11 x 8 75 x 5 5 in WEIGHT 4 5 kg 10 Ib Order Product PC1 55 2222 Pump 22 Infusion Only with Standard 2 Syringe Rack PC1 55 2275 Pump 22 Infusion Only with Anti Siphon Standard 2 Syringe Rack PC1 55 5920 Pump 22 Infusion Only with 6 10 Multi Syringe Rack PC1 55 2314 Pump 22 Infusion Only with 4 x 140 Multi Syringe Rack PC1 55 4150 Pump 22 Infusion Only with Microliter Rack PC1 55 2226 Pump 22 Infusion Withdrawal with Standard 2 Syringe Rack PC1 55 2219 Pump 22 Infusion Withdrawal with 6 10 Multi Syringe Rack PC1 55 2316 Pump 22 Infusion Withdrawal with 4 x 140 Multi Syringe Rack PC1 55 4153 Pump 22 Infusion Withdrawal with Microliter Rack PC1 70 2222 PHYSIO 22 Infusio
13. 0 2110 PC1 71 2110 MICROLITER RACK PC1 70 2105 PC1 71 2105 PC1 70 2108 PC1 71 2108 PC1 70 2111 PC1 71 2111 Push Pull Syringe Pumps STANDARD PC1 70 2020 PC1 71 2020 PC1 70 2019 PC1 71 2019 REMOTE PC1 70 2120 PC1 71 2120 PC1 70 2119 PC1 71 2119 Infusion Only models do not include an anti siphon bracket or a retaining bracket These can be ordered as a special Harvard PHD 22 2000 Pump Series Kits amp Upgrades Continuous Flow Tubing Segment ORDER PRODUCT This continuous flow tubing segment is used with the PHD 22 2000 Push Pull Syringe Pump When used with the programmable model it makes continuous 24 7 flow possible Syringe Rack Kits PC1 70 2012 PHD 22 2000 6 10 Multi Syringe Rack PC1 70 2013 PHD 22 2000 4 x 140 Multi Syringe Rack PC170 2014 PHD 22 2000 Microliter Multi Syringe Rack Specifications PC1 70 2015 PHD 22 2000 Anti Siphon Kit Infusion Only Pump TUBING 0 062 in ID Tygon tubing Upgrades TUBING LENGTH 3 x 112 in sections PC1 70 2016 PHD 22 2000 Infusion Only to Infusion Withdrawal MAX PRESSURE 15 p s i PC1 70 2017 PHD 22 2000 Infusion Withdrawal to Programmable VALVE MATERIALS Polycarbonate silicone PC1 70 2018 PHD 22 2000 Infusion Only to Programmable 1 These multiple syringe racks will fit any PHD 22 2000 series syringe pump listed above and are easily interchangeable Order Product Upgrades are available for Infusion Only and Infusion Withdrawal models of PHD 22 2000 series pumps All upg
14. 0 Syringe Pump Module CC KEY FEATURES Accuracy of 0 35 Q Footprint 11 75 x 5 5 x 6 5 in Q 3 06 pl min to 215 8 ml min Q CE ETL UL CSA WEEE EU RoHS amp CB Scheme TYPE ACCURACY REPRODUCIBILITY SYRINGE TYPE SYRINGE SIZE Size Minimum Size Maximum FLOW RATE Minimum Maximum AVERAGE LINEAR FORCE CONNECTORS RS 232 RS 485 USB 1 0 amp TTL DIMENSIONS Overall HxWxD WEIGHT Specifications Microprocessor single syringe infusion withdrawal 0 35 0 05 Plastic glass or stainless steel 0 5 ul 140 ml 3 06 pl min 215 8 ml min 91 kg 200 Ibs 100 Force Selection 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 29 8 x 14 0 x 16 5 cm 11 75 x 5 5 x 6 5 in 5 3 kg 11 2 Ibs REGULATORY CERTIFICATIONS CE ETL UL CSA WEEE EU RoHS amp CB Scheme Order PC1 70 3510 Product PHD ULTRA 4400 Syringe Pump Module with Power Supply fax 508 429 5732 www harvardapparatus com
15. 0 multi rack holds four 60 ml or 140 ml plastic syringes only e 6 10 multi rack will hold up to 10 syringes from 0 5 pl to 20 ml and up to 6 syringes from 30 ml to 60 ml e The microliter syringe rack independently holds 4 microliter sy ringes from 0 5 pl to 10 ml enabling syringes of different sizes to run simultaneously This pump features an LED display and numeric keypad for easy entry of syringe diameter data and flow rates Flow rate units can be set in ul hr ul min ml hr and ml min An optical encoder monitors leadscrew rotation to accurately maintain any flow rate The run LED flashes when syringe plunger movement stops unexpectedly A complete line of accessories for the Pump 22 are available including a footswitch audible alarm reversing switch and serial cables see page 42 The Pump 22 can be controlled using RS 232 serial commands Multiple syringe pumps can be interconnected by daisy chaining pumps Up to 100 pumps can be addressed independently using internal reference addresses from O to 99 The default setting is O A set of sample programs using the basic programming language is included with each pump PHYSIO 22 The PHYSIO 22 Pump delivers high accuracy pulseless flow with no electrical noise to interfere with the sensor signal while performing physiological analyses This specialty pump is based on our legendary Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 Syringe Pump 22 but with a
16. 3 U O cr o D lt zi va D I E 3 U SYRINGE PUMPS PHD 22 2000 MRI Compatible Syringe Pump PHO 2000 infuse Withdraw HARVARD ENTER mivani 44 000 Prano Mada Control Box KEY FEATURES High accuracy and precision Can be placed near an imaging magnet Holds 0 5 yl to 140 ml glass or plastic syringes Legendary reliability 2 year warranty BENEFITS Injecting Anesthesia Q Drug Delivery Injecting Dyes The MRI Compatible Pump is based on the design of one of our most tried and trusted syringe pumps The PHD 22 2000 This pump is made of mostly non magnetic materials and can be placed near to an imaging magnet when it is in use The control box should be positioned at least 5 feet away from the opening of the magnet and not in direct line of the opening The exact distance is dependent upon the strength of the magnet This will now make drug or dye perfusions possible while the magnet is on This pump is a remote model where the syringe pumping mechanism is separated from the control box by a 30 foot cable This allows the pump to be started and stopped near the magnet via a RUN STOP switch that was added to the syringe holder If additional Space Is required between the control box and pumping mechanism a 60 foot cable is also available For more details on the PHD 22 2000 Pump Line see pages 29 to 30 Program Description The programming functions of this pump provide powerful capabil
17. 377 ks 302189 6 4 kg 14 Ibs 4 5 kg 10 Ibs 15 29 e 29 16 22 22 16 Our technical support specialists are eager assist you with product selection amp questions call today amp Harvard Apparatus phone 508 893 8999 toll free US 800 272 2775 fax 508 429 5732 www harvardapparatus com 0 lt zu Z 0 m U C lt U 0 n D Q ct O 2 0 c Q N lt a Z m T C lt U U 9 O E O 5 O E Q D SYRINGE PUMPS Choosing the Right Pump for Your Application amp Budget continued Find your application and go to the pages indicated for more information Application Guide STANDARD SYRINGE PUMPS SEE PAGE ACCURATE DELIVERY OF COATINGS ANIMAL FEEDING BULK FLUID TRANSFER CELL CULTURES CELLULAR INJECTION CONTINUOUS INFUSIONS DOPING DRUG DELIVERY SAME INFUSION RATES DRUG DELIVERY DIFFERENT INFUSION RATES DRUG DELIVERY TIME RELEASED DRUG DEVELOPMENT ELECTROSPINNING FLUID BLENDING FLUID BLENDING 2 INDEPENDENT CHANNELS FLUID SAMPLING GRADIENTS HIGH PRESSURE INJECTION HIGHLY CORROSIVE FLUIDS HPLC INJECTING INTO HIGH PRESSURE REACTION VESSELS INJECTION PRESSURE CALCULATIONS INSTRUMENT INJECTIONS LOW PRESSURE CHROMATOGRAPHY MASS SPECTOMETRY MEDICAL COATING DELIVERY MICRODIALYSIS MICROFLUIDICS MRI STUDIES MULTIPLE SIMULTANEOUS FEEDING STATIONS NANOFLUIDICS NUTRITIONAL STUDIES OEM MODULES OOCYTE APPLICATIONS PATCH CLAMP
18. 5 E E PC170 3006 PC1 70 3306 PC1 70 3406 PC1 70 3506 PC1 70 3007 PC1 70 3307 Remote Satellite Module PC1 70 3008 PC1 70 3308 PC1 70 3408 PC1 70 3508 PC1 70 3009 PC1 70 3309 PC1 70 3410 Remote Satellite Module PC170 3010 PC1 70 3110 PC1 70 3410 PC1 70 3510 PC1 70 3111 PC1 70 3112 Note Gradient Systems are available see page 21 for details 20 Harvard Apparatus phone 508 893 8999 Syringe Pump Syringe Pump toll free U s 800 272 2775 Order Product PHD ULTRA Options PC1 70 3030 PC1 70 3033 PC1 70 3031 PC1 70 3032 PC1 70 3034 RS 232 RJ 11 Connectors Option daisy chain Analog Control Input Option 0 10V 3 Internal Pinch Valve Option Internal 3 Way Isolation Valve Option Internal Fan Option PHD ULTRA Syringe Rack Kits and Upgrades PC1 70 3020 PC1 70 3021 PC1 70 3022 PC1 70 3023 PC1 70 4010 PC1 70 4011 PC1 70 4012 6 10 Multi Syringe Rack for PHD ULTRA 4x140 Multi Syringe Rack for PHD ULTRA Microliter Rack for PHD ULTRA holds 4 syringes Anti Siphon Kit for PHD ULTRA Upgrade Infusion Only to I W Upgrade Infusion Only to Programmable Upgrade I W to Programmable I Note Requires Return to Factory Note Only for available for Infusion Withdrawal or Programmable Models 3 Note Only for Programmable Models Not available on Satellite Boxes Note Fan option is required if external operating ambient is expected to be 35 C Acces
19. 6 Product PHD ULTRA Syringe Pump Module with Power Supply fax 508 429 5732 www harvardapparatus com 37 0 lt a Z m U C lt U O m lt 3 Q 0 o D o D 0 lt J 5 va D T c 3 o T N lt a Z O m U C lt U 7 O m lt O I Q o Oo D o D lt 2 va D D c 3 U SYRINGE PUMPS Customized Solutions OEM and Special Syringe Pumps continued OEM Modules PHD ULTRA Push Pull Syringe Pump Module KEY FEATURES Accuracy of 0 25 Q Footprint of 6 6 x 5 5 x 11 8 in Q 0 001 yl hr to 220 82 ml min WEEE RoHS ETL UL CSA Specifications TYPE Microprocessor four syringe push pull infusion withdrawal ACCURACY 0 25 REPRODUCIBILITY 0 05 SYRINGE TYPE Plastic glass or stainless steel SYRINGE SIZE Size Minimum 0 5 ul Size Maximum 140 ml FLOW RATE Minimum 3 06 pl min Maximum 215 8 ml min AVERAGE LINEAR FORCE 34 kg 75 Ibs 100 Force Selection 30 VDC Input CONNECTORS RS 232 9 pin D Sub Connector RS 485 IEEE 1394 6 pos USB Type B 1 0 amp TTL 15 pin D Sub Connector DIMENSIONS Overall Hx WxD 29 8 x 14 0 x 16 5 cm 11 75 x 5 5 x 6 5 in WEIGHT 5 1 kg 11 2 Ibs REGULATORY CERTIFICATIONS CE ETL UL CSA WEEE EU RoHS amp CB Scheme Order PC1 70 3508 Product PHD ULTRA Push Pull Syringe Pump Module with Power Supply Harvard Apparatus phone 508 893 8999 toll free US 800 272 2775 OEM Modules PHD ULTRA 440
20. 7 7 58xl15emit9x 7xo in 2 1 kg 4 6 Ibs CE ETL UL CSA WEEE EU RoHS amp CB Scheme Pump 11 Pico Plus Elite Infusion Withdrawal Programmable Dual Syringe fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS NEW Pump 11 Elite Nanomite KEY FEATURES Easy to use LCD color touch screen with GUI interface Q Light weight makes it ideal for hand held or stereotaxic injection Easily program simple to complex Methods without a PC Create and store up to 2 Methods APPLICATIONS Cellular Injections Drug Delivery Microinjections Hand Held Automated Delivery Stereotaxic Injections Regenerative Medicine The Pump 11 Elite Nanomite is a single syringe infusion withdrawal programmable syringe pump This pump allows you to create save and run simple to complex Methods without a PC The flow rate range is 3 66 pl min to 3 818 ml min with 11 Ibs of adjustable force across the entire flow rate range The Pump 11 Elite Nanomite has a footswitch input USB serial port for computer control RS 485 ports for daisy chaining pumps and Digital I O for external control via an independent computer or device There is also an option for daisy chaining pumps through the RS 232 RJ 11 ports This option must be ordered at the time the pump is ordered See page 13 for more information on connectivity This pump consists of a control unit an injection unit a 6 foot cable to connect the two units and a footswitch For more
21. Applications in this area include microdialysis and site specific Delivering accurate volumes and flows is critical to maintaining microinjection into various brain regions dosing regimens interpretation of pharmacokinetics and other data e For stereotaxically guided single channel microinjection e For single or dual channel infusions see the Pump 11 Elite see the Pump 11 Elite Nanomite on page 15 or the PHD on page 11 or PHD ULTRA on page 16 ULTRA Nanomite on page 23 e If MRI or PET studies are being conducted see the PHD e For single or dual probe microdialysis see the Pump 11 22 2000 on page 28 Elite on page 11 or Pico Plus Elite on page 14 e To run multiple studies from a single pump see the PHD e For applications using a liquid switch or for running ULTRA on page 16 multiple experiments simultaneously see the PHD ULTRA on page 16 1ml Syringe 21 ul min E 22 E 5 u References References PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR PROC SOC PHOTO OPT INSTRUM ENG 2008 6854 68540H Volume 89 Issue 1 March 2008 Pages 17 22 doi 10 1117 12 768798 J Appl Physiol 106 1793 1799 2009 EUROPEAN JOURNAL OF PHARMACOLOGY EXPERIMENTAL NEUROLOGY Volume 602 Issue 1 5 January 2009 Pages 112 116 Volume 210 Issue 2 April 2008 Pages 514 520 ACTA ANAESTHESIOLOGICA SCANDINAVICA Volume 52 Issue 2 pages 289 294 February 2008 8 Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www
22. C1 72 0088 MFRP10 Infusion Syringe Pump 110 VAC PC1 72 0089 MFRP10 Infusion Syringe Pump 220 VAC fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS NEW Model 55 Pump 150ml QD 1N 0 0 ml gt hute 1 0000 misli Flou 1 0000 wih Stop KEY FEATURES Accurate and smooth flow Syringe detection Advanced occlusion detection Battery back up to AC power Wireless controller option The Model 55 Syringe Pump is an easy to use syringe pump that can control the injection rate and total volume precisely and continuously over long periods of time This pump has an optional wireless controller that allows you to control infusion and monitor status remotely Specifications INJECTION ACCURACY 3 APPLICABLE SYRINGES 5 ml 10 ml 20 ml 30 ml and 50 ml FLOW RATE 5 ml Syringe 0 1 100 ml hr 10 ml Syringe 0 1 100 ml hr 20 ml Syringe 0 1 400 ml hr 30 ml Syringe 0 1 600 ml hr 50 ml Syringe 0 1 1500 ml hr INJECTION INCREMENT 0 1 ml hr FAST INJECTION Exhaust impingement quantity of fast injection QUANTITY 10 m 20 ml 50 ml syringe 1 0 m KVO SPEED 0 1 5 0 ml hr ADJUSTABLE RANGE FAST INJECTION SPEED 10 ml syringe 100 ml h 20 ml syringe 200 ml h 50 ml syringe 500 ml h Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 0 lt a Z C m U C lt U 0 lt O Q o gi Ol U L 3 o Specifications continued Alarm indication sent to infusion supervision management syste
23. E Size Minimum Size Maximum FLOW RATE Minimum Maximum AVERAGE LINEAR FORCE CONNECTORS RS 232 DC Power POWER DIMESIONS Overall Hx WxD Mounting WEIGHT Order PC1 72 2219 PC1 72 2225 Product Microprocessor single syringe infusion withdrawal 0 5 0 1 Glass or plastic 0 5 ul 50 60 ml 0 001 ml hr 44 283 ml min 11 36 kg 25 Ibs 4 pin RJ11 telephone jack dual RS 232 ports 2 pin Header friction lock 15 to 40VDC 12W min user supplied 13 5 x 24 1 x 10 8 cm 5 3 x 9 5 x 4 25 in 22 9 x 9 5cm 9 0 x 3 75 in Mounting holes for 4 8 Screws 1 27 kg 2 8 Ibs Milliliter Syringe Pump Module without Power Supply Milliliter Syringe Pump Module with Power Supply fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS Customized Solutions OEM and Special Syringe Pumps continued OEM Modules PHD ULTRA Syringe Pump Module OEM Modules High Force Syringe Pump Module KEY FEATURES Accuracy of 0 25 Q 11 75x5 5x 6 5 in KEY FEATURES Accuracy of 0 5 Q Footprint of 6 6 x 5 5 x 11 8 in Q 0 001 ul hr to 220 82 ml min Q WEEE Eu RoHS ETL UL CSA Specifications TYPE ACCURACY REPRODUCIBILITY SYRINGE TYPE SYRINGE SIZE Size Minimum Size Maximum FLOW RATE Minimum Maximum AVERAGE LINEAR FORCE CONNECTORS DC Power RS 232 User 1 0 DIMENSIONS Overall Hx WxD Mounting Control Board Mounting WEIGHT Microprocessor single syringe infusion w
24. ING REMOTE PUMPING OF HAZARDOUS MATERIAL STEREOTAXIC INJECTIONS TITRATIONS VISCOUS SOLUTIONS NOTE Can be done using RS 232 Computer Control Harvard Apparatus phone 508 893 8999 Pump 11 Elite iit X X X X X X X X X X X Pump 11 Pump 11 toll free U S 800 272 2775 Pico Plus Elite Elite Nanomite HU 22 14 15 26 X X X X X X X RS 232 X X RS 232 X X X X RS 232 X X X X X X X X X X X RS 232 X X X X X X X RS 232 X Pump 33 20 x lt gt lt Xx XxX PHD 22 2000 29 X X X RS 232 X X RS 232 X RS 232 X X fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS Choosing the Right Pump for Your Application amp Budget continued OEM SYRINGE PUMP MODULES N lt a Z G m U C lt U i gt U po Q ct O 53 M c Q o PHD ULTRA eee MRI PHD 22 2000 PHD ULTRA HPSI ii p CO MICROLITER MILLILITER Bl 16 16 28 22 22 34 34 34 X X X X X X X X X X X X X X X RS 232 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 7 SYRINGE PUMPS Application Selection Guide 0 lt a Z 0 m U C lt U 0 o jol O ct O 2 U D Q et O 2 c Q Neuroscience Infusion and Drug Delivery
25. Organ Temperature Comsuption Consumption 96 Change Q 20 Increase in Survivability 76 Lower O Consumption Kidney 37 to 18 3 7 to 0 9 2 8 15 7 Rate at 18 C vs 37 C Liver 37 to 18 3 0 to 0 6 244 80 Flow ramping to reduce injection site blowback Heart 3 to 18 2 4 to 0 7 L7 70 8 Programmed methods for automatic recall of entire Brain 37 to 18 1 910 0 4 1 5 78 9 cell delivery system Up to 100 methods stored for recall Muscle 37 to 18 0 8 to 0 2 0 6 15 Q 2 year warranty Skin 37 to 18 0 4 to 0 1 0 3 75 High accuracy delivery between 367 4 pl min to 1 906 ml min Preprogrammed bolus injection mode just specify injection size and time of dispense Footswitch activation keeps hands free IR Temperature Image NanoCool at 18 C NanoCool Cell Delivery System When injecting stem cells viability of cells can be a major issue The Harvard Apparatus NanoCool is the only cooled cell injection system with optional cooled and oxygenated vortex mixer The injection system and sample vortex holder are held at 18 C The cell delivery is totally integrated in it operation through the NanoCool s microprocessor system power temperature injection volumes and flow ramping are all controlled by the NanoCool s program Keeping Specifications the cells at 18 C instead of 37 C reduces O consumption of cells by ACCURACY 40 5 an average of 76 The chart to the right shows the different organ REPRODUCIBILITY 40 0594 utilizati
26. R 12 30 VDC INPUT POWER CONNECTION 2 5 mm ID x 5 5 mm OD male plug POWER SUPPLY 100 240 VAC 50 60 Hz 8 Watts Universal Power DIMENSIONS H x W x D WEIGHT REGULATORY CERTIFICATIONS Harvard Apparatus Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 22 6 x 17 78 x 15 cm 9x7 x61n 2 1 kg 4 6 Ibs CE ETL UL CSA WEEE EU RoHS amp CB Scheme phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 13 0 lt a Z m U C lt U 0 U c j 5 T Q U c 0 m o SYRINGE PUMPS NEW Pump 11 Pico Plus Elite CE KEY FEATURES Easy to use touch screen and icon interface Outstanding flow performance Easily run simple to complex methods without a PC Alphanumeric keypad for easy Method naming and recall Adjustable linear force up to 35 Ibs Upgrade new versions of software remotely Legendary reliability 2 year warranty APPLICATIONS Microfluidics Drug Nutritional Delivery Microdialysis HPLC Cellular Injections Mass Spectrometry The Pump 11 Pico Plus Elite is the lowest flow highest accuracy syringe pump It offers enhanced flow performance with high accuracy and smooth flow from 0 54 pl min to 11 70 ml min This dual syringe pump offers unparalleled ease of use with a high resolution color touch screen with intuitive icon interface The Pump 11 Pico Plus Elite allows you to create save and run simple to complex methods without
27. RINGE PUMPS PHD 22 2000 Advanced Syringe Pumps PHD 22 2000 Infusion Withdrawal with Standard 2 Syringe Rack PHD 22 2000 Infusion Withdrawal with 6 10 Multi Rack PHD 22 2000 Infusion Only with Standard 2 Syringe Rack KEY FEATURES High accuracy and precision Multiple configuration available Smooth flow over a wide flow rate range Multi syringe racks for large capacity reservoir Legendary reliability 2 year warranty APPLICATIONS Drug Nutritional Delivery Q Electrospinning Q Microfluidics Nanofluidics Q Mass Spec Calibration Over forty years ago Harvard Apparatus perfected the leadscrew principle and created the first syringe pump Since that time tens of thousands of Harvard Apparatus pumps have earned a reputation as the most reliable research pumps in every major laboratory in the World The PHD 22 2000 syringe pump series gives you the lowest flow rates the highest accuracy the smoothest flow advanced programmability from the keypad and yet is very easy to use It is also incredibly quiet so it won t disturb your experimental subjects Configurations This pump is available as a standard push pull remote high pressure or with multi racks Upgrade We offer pumps that can be upgraded If you buy an infusion withdrawal pump and later decide you want programmability you can upgrade it see page 30 Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 PHD 22 2000 Push Pull S
28. S Volume 29 Issue 14 pages 2939 2944 No 14 July 2008 June 6 2008 The Journal of Biological Chemistry 283 15816 15824 MATERIALS SCIENCE AND ENGINEERING C Volume 29 Issue 4 5 May 2008 Pages 1293 1301 PHD ULTRA 10mi Syringe 100 nlimin Chemical and Industrial Electrospinning Where chemical synthesis pressurized systems and other applications demand high performance Harvard Apparatus has the right solution e For high pressure applications consider the PHD ULTRA HPSI with stainless steel syringes on page 22 or the PHD ULTRA 4400 on page 22 Harvard Apparatus pumps are used in creating Nano and Micro scale polymer fibers in a high voltage field The stability of flow and flexibility of use in the References JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY Volume 19 Issue 3 March 2008 Pages 332 343 PHD ULTRA series pumps INTERNATIONAL JOURNAL OF MASS SPECTROMETRY makes it easy to alter the Volume 283 Issues 1 3 1 June 2009 Pages 161 168 variables relating to JOURNAL OF MASS SPECTROMETRY Volume 43 Issue 10 pages 1413 1420 October 2008 successful electrospinning Our pumps include a grounded power supply and can be supplied with a grounding strap which will shunt any high voltage arcs to ground References Anal Chem 2008 80 9 pp 3112 3122 Muhammad Miftahul Munir et al 2008 Nanotechnology 19 145603 Journal of Biomechanics Volume 42 Issue 6 Pages 722 730 16 April 2009 Ha
29. Y REPRODUCIBILITY SYRINGE Type P Com B Normally Open A Normally Closed Output Specifications Microprocessor dual drive single syringe infusion withdrawal 0 35 0 1 Plastic glass or stainless steel Size Minimum 0 5 ul Size Maximum 140 ml FLOW RATE Minimum 0 0004 ul hr Maximum 106 6 ml min CONNECTORS RS 232 RJ11 4 conductor TTL 9 pin connector AVERAGE LINEAR FORCE 5 7 Ibs STEP RESOLUTION 0 33 um step VOLTAGE RANGE 95 to 130 VAC 60 Hz 220 to 260 VAC 50 Hz selectable DIMENSIONS HxWxD 152x31 1x28 6cm 6 x 12 5 x 11 25 in WEIGHT 6 8 kg 15 Ib Order Product PC1 55 3333 Pump 33 Infusion Withdrawal Dual Syringe References Chen D Wendt C H and Pui YH D A novel approach for introducing bio materials into cells Journal of Nanoparticle Research 2000 Vol 2 133 139 Cabrales P et al Oxygen transport by low and normal oxygen affinity hemoglobin vesicles in extreme hemodilution Am J Physiol Heart Circ Physiol 2005 Vol 288 H1885 H 1892 Lee S H et al A polymer lab on a chip for reverse transcription RT PCR based point of care clinical diagnostics Lab on a Chip 2008 Vol 8 2121 2127 fax 508 429 5732 www harvardapparatus com 27 N lt a Z O m TU C lt T 7 T es 3 U W W g C 2 0 lt i va D c 3 U N lt a Z m T C lt T T I 0 N M N eo o O lt a Q O
30. ach pump used ja LO A Ld e 3 E MEL AMEN me k 4 4 da I im HARVARD fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS NEW Pump 11 Elite CE KEY FEATURES Easy to use touch screen and icon interface Outstanding flow performance Easily run simple to complex methods without a PC Alphanumeric keypad for easy Method naming and recall Q Adjustable linear force up to 35 Ibs Upgrade new versions of software remotely Q Legendary reliability 2 year warranty APPLICATIONS Microfluidics Drug Nutritional Delivery Microdialysis Emulsification Bioreactors Electrospinning Mass Spectrometry The Pump 11 Elite Series of syringe pumps expands its capabilities to Satisfy your experimental requirements These compact syringe pumps carry on the tradition as the premier workhorse infusion pump offering unparalleled ease of use with a high resolution color touch screen with intuitive icon interface The Pump 11 Elite Series allows you to create save and run simple to complex methods without a PC Superior Performance These syringe pumps have a new mechanism that includes a tight gripping more secure syringe clamp for syringes ranging from O 5ul to 60ml single syringe and O 5ul to 10 ml dual syringe The Pump 11 Elite Series offers enhanced flow performance with high accuracy and smooth flow from 1 28 pl min to 88 28 ml min 25 99 ml mi
31. aratus phone 508 893 8999 PC1 70 2208 Single Syringe N lt a Z O m U C lt U 0 7 t 3 U J o Specifications TYPE Microprocessor single or dual syringe infusion only or infusion withdrawal ACCURACY 0 5 REPRODUCIBILITY 0 1 SYRINGE Type Plastic or glass Size single syringe 0 5 ul to 50 60 ml Size dual syringe 0 5 ul to 10 ml FLOW RATE Single Syringe 0 0014 ul Rr to 26 56 ml min Dual Syringe 0 0014 ul hr to 7 91 ml min CONNECTORS RS 232 Advanced models only TTL No AVERAGE LINEAR FORCE 7 27 kg 16 Ibs STEP RESOLUTION 0 33 um step VOLTAGE RANGE Universal input 100 240 VAC 50 60 Hz 18 watts Use POWER SUPPLY only Harvard Apparatus approved supply and line cord DIMENSIONS Hx WxD 13 x 22 9 x 11 4 cm 5x9 x 4 5 in WEIGHT 2 1 kg 4 6 Ib Order Product PC1 70 2208 Pump 11 Plus Infusion Only Single Syringe PC1 70 2209 Pump 11 Plus Infusion Only Dual Syringe PC1 70 2211 Pump 11 Plus Advanced Infusion Withdrawal Single Syringe with Dual RS 232 PC1 70 2212 Pump 11 Plus Advanced Infusion Withdrawal Dual Syringe with Dual RS 232 References Migu ns M et al Glutamate and aspartate levels in the nucleus accumbens during cocaine self administration and extinction a time course microdialysis study Psychopharmacology 2008 Vol 196 303 313 Russom A et al Microfluidic Leukocyte Isolation for Gene Expression Analysis in Critically Ill Hospitalized Patients Clinical Chemi
32. d Alone CE KEY FEATURES Delivers gt 200 Ibs 91 kg linear pumping force across a wide flow rate range Specifications TYPE ACCURACY REPRODUCIBILITY SYRINGES MIN MAX FLOW RATE Minimum Maximum CONNECTORS RS 232 RS 485 USB 1 0 amp TTL AVERAGE LINEAR FORCE VOLTAGE RANGE DIMENSIONS H x W x D Control Box Remote Box WEIGHT Accurate and smooth flow Ideally suited for stainless steel syringes Easy to use touch screen and icon interface Program simple to complex Methods without a PC Legendary reliability 2 year warranty Stand Alone Microprocessor single syringe infusion withdrawal programmable 0 35 0 05 0 5 ul to 140 ml 3 06 pl min 215 8 ml min 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 91 kg 200 Ibs 100 Force Selection 1 8 Stepper Motor 100 240 VAC 50 60 Hz 50 W 0 5 A fuse 30 48 x 21 59 x 18 4 cm 12x 8 5 x 7 25 in N A 5 5 kg 12 1 Ib Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 PHD ULTRA HPSI Remote CC KEY FEATURES Remote Microprocessor single syringe infusion withdrawal programmable 0 35 0 05 0 5 ul to 140 ml 3 06 pl min 215 8 ml min 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 91 kg 200 Ibs 9 100 Force Selection 1 8 Stepper Motor 100 240 VAC 50 60 Hz 75 W 0 5 A fuse 30 5 x 21 6 x 10 8 cm 12x 8 5 x 4 25 in 27 9 x 13 5 x 18
33. dient Stepped cte Continuous Time HARVARD APPARATUS 10 27 09 11 57 15 AM Enter your total time and number of steps and then press the Enter button green check mark to accept or press the Cancel button red X to cancel and return to the previous screen Microprocessor multiple syringe infusion only infusion withdrawal or infusion withdrawal programmable 0 25 0 05 Plastic glass or stainless steel 0 5 nl 140 ml 1 56 pl min 215 8 ml min 4 3 WQVGA TFT Color Display with Touchpad 9 pin D Sub Connector 6 position IEEE 1394 Type B 15 pin D Sub Connector Phono jack 34 kg 75 Ibs 100 force selection 0 082 um step Universal input 100 240 VAC 50 60 Hz 10 16 x 30 48 x 21 59 cm 4 x 12 x 8 5 in 4 5 kg 10 Ibs CE ETL UL CSA WEEE EU RoHS amp CB Scheme Product PHD ULTRA Gradient System 1 Master 1 Satellite with Stand PHD ULTRA Gradient System 1 Master 2 Satellites with Stand PHD ULTRA Gradient System 1 Master 1 Satellite without Stand PHD ULTRA Gradient System 1 Master 2 Satellites without Stand fax 508 429 5732 www harvardapparatus com 21 iU lt a PA m U C lt U 0 X cr 3 Q g O N D g o D 3 lt o lt D 3 6 lt 7 Z m U C lt U 6 U L U C F AJ gt IL U f Q I Q U L U C T AJ gt A A O O 22 SYRINGE PUMPS NEW PHD ULTRA HPSI and PHD ULTRA 4400 PHD ULTRA 4400 Stan
34. ethod 2 Enter operating parameters Direction Set pump to infuse or withdraw Control input Trigger Input Connect and external device to start or stop a pump or Method Footswitch Input Start and stop a pump Trigger 1 Ouput Signal another device to start and stop a pump or Method Trigger 2 Output Signal another device to start and stop a pump or Method Sync Output Synchronize other devices Valve Output External valve control Run Indicator Connect an external LED or monitoring device to a pump ANALOG CONTROL Analog control of the motor speed O to 10 v This option OPTIONAL must be ordered at the same time the pump is ordered 3 Preview or Run your Method Quick Start Methods are for simple infusions withdrawals or a combination depending on the pump model Custom user defined Methods can be created when a more advanced Method is required The setup for a custom Method is easy using the standard profiles found on all Infusion Withdrawal and Infusion Withdrawal Programmable PHD ULTRAS The list of available profiles are o lt a Z m U C z U o U ai U C T AJ gt gt Q lt Q O o Q o lt I va o c 5 U o0 222229 LETETTE sereesnnenne SJETTE DIT LONE By programming and saving custom Methods in the pump multi user errors are reduced Easily transfer complex methods to other pumps and or download methods from a PC Forget having to duplicate method development efforts for each n
35. ew pump added to your system USER In addition to the advanced pumping profiles listed above the PHD 1 0 CONNECTOR ULTRA contains a variety of advanced options allowing the user to repeat steps link methods control valves external triggers etc ANALOG CONTROL CONNECTOR optional Advanced Programming Features DIGITAL I O Flow Programming Change the flow with time volume or a triggered event as many times as you like Bolus nject a large volume of drug or drugs at once The bolus injection can be made in time or volume Concentration Delivery Calibrate flow in concentration units of mg kg easily so flow is calibrated to concentration of drug and animal weight Gradients EZ PRO software allows you to easily program gradients continuous or stepped Ratio Up to three solvents I O Dedicated and user defined I O Pulsed Flow So you can program the pulse easily CONNECTORS for pump to pump communication SERIAL INPUT Advanced Connectivity eee eee All PHD ULTRA Syringe Pumps come standard with a footswitch de from PC USB RS 232 RS 485 and I Q connectors There IS also an option i amp arcorimunication FOOTSWITCH for RJ 11 connectors and analog control These options have to be from PC INPUT ordered at the time the pump is ordered switch sold separately 18 Harvard Apparatus phone 508 893 8999 toll free US 800 272 2775 fax 508 429 5732 www harvardapparatus
36. f Volume O ml Force Level 100 Alarms There are several alarms available on the Model 11 Elite Syringe Pumps e End of Run e Near End of Run e Power Up e Stall Advanced GLP Documentation Features e Download experimental parameter information to PC e Alphanumeric keypad for method naming Adjustable Force The maximum linear force of the Pump 11 Elite is 16 kg 35 Ibs Depending upon the syringes you are working with and your overall experimental setup you may want to adjust the amount of force applied to the pusher block You can select a percentage of the total force from 20 to 100 Refer to the user s manual for suggested force level settings CONTROL MULTIPLE PUMPS VIA DAISY CHAIN WITH RS 485 Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS NEW Pump 11 Elite continued CONNECTORS for pump to pump communication Advanced Connectivity J The infusion only Pump Syringe 11 Elite Pumps come standard with a A Footswitch input and USB connector The infusion withdrawal Z programmable Pump 11 Elite Syringe Pumps include a Footswitch input USB RS 485 and I O connectors There is also an option for RJ 11 TU connectors on the programmable pumps This option has to be ordered at C the time the pump is ordered 0 E OPTIONAL 3 RJ 11 m D FOOTSWITCH USB SERIAL RS 485 UNIVERSAL INPUT INPUT CONNECTORS POWER INPUT fo
37. harvardapparatus com SYRINGE PUMPS Application Selection Guide continued Cell Biology Mass Spectrometry Calibration and Electrospray lonization Cellular studies typically involve the injection of very small volumes under controlled conditions Several models offer a foot pedal EE MIO on command Calibration and Electrospray lonization Where precise volume delivery and pulse free flow are required for optimum instrument calibration or solvent elimination the Pump 11 series has long been the industry standard for manufacturers in OEM configurations and end users See the Pump 11 Elite on page 11 and Pump 11 Pico Plus Elite systems on page 14 e For cellular microinjections of plasmids viruses and the like see either the Pump 11 Pico Plus Elite on page 14 PHD ULTRA Nanomite on page 23 or the Pump 11 Elite Nanomite on page 15 Additionally use of the gradient capabilities of the Pump 11 Elite or PHD ULTRA Series allows for automated multi point calibration when performing GLP studies MALDI TOF Matrix Addition Using the Nanomite with its detachable delivery and electronics allows for easy placement of the syringe over the target to facilitate the dispensing of the appropriate matrix See the PHD ULTRA Nanomite on page 23 or the Pump 11 Elite Nanomite on page 15 0 lt zu Z M I U C lt U 0 gt o jo O ct O 2 V D Q et O 2 0 C Q References ELECTROPHORESI
38. hod up to 800 steps Infusion Withdrawal Programmable This model supports infuse only withdraw only infuse withdraw and withdraw infuse operations Users can create and store multiple Methods of up to 800 steps on the pump Harvard Apparatus phone 508 893 8999 toll free US 800 272 2775 fax 508 429 5732 www harvardapparatus com 1 o lt 7 Z m U C lt U o U L U C T AJ gt gt Q lt Q I O Q o lt I va 0 3 U o0 SYRINGE PUMPS NEW PHD ULTRA Advanced Syringe Pumps continued FOOTSWITCH INPUT Start and stop a pump USB AND RS 232 Control your pump with a computer SERIAL INPUTS RS 485 CONNECTORS Connect multiple pumps together up to 99 Connect remote mechanism HARVARD APPARATUS Constant 04 28 09 1 21 10 PM Rate Ramp Select the desired step profile or press the Cancel button red X Stepped Pulse to return to the previous screen Connect satellite pumps to the Master pump for binary or ternary gradient system composition RJ 11 CONNECTORS Connect multiple pumps together daisy chain OPTION USER 1 0 CONNECTOR Concen Bolus tration Autofill Advanced Options E Program Description To operate the PHD ULTRA the user defines all the required parameters for infusing and or withdrawing liquids through a Method This may be a Quick Start Pre Programmed or User Defined Method The basic operation is a simple 3 step procedure 1 Select a M
39. inear Force MICROLITER SYRINGE PC1 70 2220 0 001 ul Rr to 6 Ibs PC or other RS 232 PUMP MODULE or PC1 70 2225 1 33 ml min external controller serial input MILLILITER SYRINGE PC1 70 2219 0 001 ml Rr to 25 Ibs PC or other RS 232 PUMP MODULE or PC1 70 2226 44 28 ml min external controller serial input HIGH PRESSURE PC1 70 2202 0 0001 ul hr to 200 Ibs PC or other RS 232 SYRINGE PUMP MODULE 220 82 ml min external controller serial input PHD ULTRA PC1 70 3506 3 06 pl min to 75 Ibs PC or other RS 232 and USB SYRINGE PUMP MODULE 215 8 ml min external controller serial input PHD ULTRA PUSH PULL PC1 70 3508 3 06 pl min to 75 lbs PC or other RS 232 and USB SYRINGE PUMP MODULE 215 8 ml min external controller serial input PHD ULTRA 4400 PC1 70 3510 3 06 pl min to 200 Ibs PC or other RS 232 and USB SYRINGE PUMP MODULE 215 8 ml min external controller serial input Drug Deposition onto Cardiac Stents 34 Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS Customized Solutions OEM and Special Syringe Pumps continued Ternary Gradient System for Liquid Chromatography 0 lt I Zz m U C lt U o O m lt O 2 o o O D 2 2 o lt 3 va D y c U N Microfluidics Lab on a Chip Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 ww w harvardapparatus com 35 N lt a Z O m U
40. ing pump operation The Run Screen shows all of the pump parameters on one screen for easy review The software is organized into three main navigational branches the quick start Methods user defined Methods and system settings You can control operations directly through the touch screen or remotely from an independent computer or device via the external I O interface fax 508 429 5732 www harvardapparatus com 11 N lt a Z O m TU C lt U 0 T a 3 U m ct D 0 lt a Z 0 m U C lt U 0 m C 3 5 m zi o SYRINGE PUMPS NEW Pump 11 Elite continued HARVARD APPARATUS Quick Start Infuse Only Enter the settings Make sure to select the right syringe BD plastic 5 ml 11 99 mm Pump Models The Pump 11 Elite Syringe Pumps are available in two configurations designed for different operating environments and varying degrees of operational flexibility 1 Infusion Only single and dual syringe models This model Supports infusion operations at user defined flow rates and with selectable target volume or time values to control the total infusion volume The Infusion Only models do not include programmable user defined Methods 2 Infusion Withdrawal Programmable single and dual syringe models This model supports infuse only withdraw only infuse withdraw and withdraw infuse operations at user defined flow rates and with selectable target volume or time This model also allows user
41. inges from 0 5 pl to 140 ml e The 4x 140 multi rack holds four 60 ml or 140 ml plastic syringes only e 6 10 multi rack will hold up to 10 syringes from 0 5 pl to 20 ml and up to 6 syringes from 30 ml to 60 ml e The microliter syringe rack independently holds 4 syringes from 0 5 ul to 10 ml enabling syringes of different sizes to run simultaneously Easy to Use Interface The PHD ULTRA Syringe Pumps are very easy to use with an LCD color touch screen and icon interface The Message Area of the touch screen is used to display helpful instructions for the currently displayed screen It is also used to display error or warning messages to indicate problem conditions in a Method or error conditions during pump operation The Run Screen shows all of the pump parameters on one screen for easy review BIS Pump Models The PHD ULTRA Pumps are available in three configurations designed for different operating environments and varying degrees of operational flexibility Infusion Only This model supports infusion operations at user defined flow rates and with selectable target volume or time values to control the total infusion volume The Infusion Only models do not include programmable user defined Methods Infusion Withdrawal This model supports infuse only withdraw only infuse withdraw and withdraw infuse operations at user defined flow rates and with selectable target volume or time Users can create and store one Met
42. ithdrawal 0 5 0 1 Plastic glass or stainless steel 0 5 ul 140 ml 0 001 ul hr 220 82 ml min 90 91 kg 200 Ibs 4 pin Header Friction lock Molex or AMP 4 pin RJ 11 Telephone Jack dual RS 232 ports 9 pin D Sub Female 16 8 x 14 0 x 30 2 cm 6 625 x 5 50 x 11 875 in 28 9 x 12 7 cm 11 375 x 5 00 in mounting holes for 4 8 screws 11 43 x 17 78 cm 4 50 x 7 00 in Mounting holes for 4 8 screws 3 86 kg 8 5 Ibs Note operating temperature may be extended with forced air cooling Order PC1 70 2202 Harvard Apparatus Product High Pressure Syringe Pump Module without Power Supply phone 508 893 8999 toll free U S 800 272 2775 3 06 pl min to 215 8 ml min CE ETL UL CSA WEEE EU RoHS amp CB Scheme TYPE ACCURACY REPRODUCIBILITY SYRINGE TYPE SYRINGE SIZE Size Minimum Size Maximum FLOW RATE Minimum Maximum AVERAGE LINEAR FORCE CONNECTORS RS 232 RS 485 USB 1 0 amp TTL DIMENSIONS Overall Hx WxD WEIGHT Specifications Microprocessor dual syringe infusion withdrawal 0 25 0 05 Plastic glass or stainless steel 0 5 ul 140 ml 3 06 pl min 215 8 ml min 34 kg 75 Ibs 100 Force Selection 30 VDC Input 9 pin D Sub Connector IEEE 1394 6 pos Type B 15 pin D Sub Connector 29 8 x 14 0 x 16 5 cm 11 75 x 5 5 x 6 5 in 5 1 kg 11 2 Ibs REGULATORY CERTIFICATIONS CE ETL UL CSA WEEE EU RoHS amp CB Scheme Order PC1 70 350
43. ities for advanced experiments While in program mode this pump can perform the following tasks at a predetermined time or when prompted by a signal from an external device e Start or stop pumping e Change pumping direction infusion withdrawal e Change flow rates e Pump a precise volume and stop e Pause operation e Ramp up or down flow rates In program mode the above tasks can be linked together into powerful programs to simplify your automation projects Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 TYPE ACCURACY REPRODUCIBILITY SYRINGE Type Size Minimum Size Maximum FLOW RATE Minimum Maximum CONNECTORS RS 232 TTL AVERAGE LINEAR FORCE STEP RESOLUTION VOLTAGE RANGE CABLE LENGTH DIMENSIONS H x W x D Control Box Syringe Holder PC1 70 2130 Syringe Holder CE Specifications Microprocessor dual syringe infusion withdrawal or infusion withdrawal programmable 0 35 0 05 Plastic or glass 0 5 jl 140 ml 0 0001 ul hr 220 82 ml min RJ11 4 conductor 9 pin D Sub Connector 50 Ibs 0 082 um step Universal input 100 240 VAC 50 60 Hz 9 1 m 30 ft 9 5 x 27 9 x 22 9 cm 3 5 x 11 x9 in 22 9 x 43 2 x 30 5 cm 9 x 17 x 12 in Order Product PHD 22 2000 MRI Compatible Remote Infusion Withdrawal Dual Syringe PC1 70 2131 PHD 22 2000 MRI Compatible Remote Infusion Withdrawal Programmable Dual Syringe fax 508 429 5732 www harvardapparatus com SY
44. m ALARM FUNCTION INFORMATION CAN BE Injection complete near completion occlusion low battery disengagement 3 independent alarms BATTERY Rechargeable lithium battery 7 4V 1600mAh ELECTROMAGNETIC Accord with the stipulation of IEC60601 1 2 WAVE HERTZIAN WAVE 1993 edition PROTECTION PROPER ENVIRONMENT Environment temperature 5 40 pressure CONDITION 70 106kPa relative humidity 3076 9076 STORAGE CONDITION Environment temperature 15 50 pressure 70 106 kPa relative humidity 30 90 ELECTRICAL SAFETY accord with the stipulation of GB9706 1 1995 MAXIMUM SIZE 288 x 130 x 122 mm OF OUTER SHELL MAXIMUM WEIGHT lt 2 5 ke POWER 50 60Hz AC85 265 V 50 60 Hz FUSES 220 V 0 2A x2 12V 2 5A x2 MAXIMUM POWER 25VA running more than two hours CONSUMPTION after being charged DIMENSIONS H x Wx D 24x 10x 15 cm 9 5 x 4 0 x 5 9 in WEIGHT 2 2 kg 4 9 Ibs Order Product PC1 72 9700 Model 55 Pump with Built in Controller l PC1 72 9702 Model 55 Pump with Wireless Monitoring fax 508 429 5732 www harvardapparatus com 33 SYRINGE PUMPS Customized Solutions OEM and Special Syringe Pumps A few of the application areas we have developed OEM solutions for include Drug deposition onto cardiac stents Liquid chromatography Lab on a chip systems for bio analysis N lt a Z O m U C lt U 7 O m lt O I Q Oo D O D 0 lt va D 7 c 3 U o Order Flow Rate Average Control Communications L
45. n for dual syringe rack Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 The Pump 11 Elite Series is available in Infusion Only or Infusion Withdrawal Programmable Models with single or dual syringe racks All Pump 11 Elite syringe pumps have a footswitch input and USB serial port for computer control The Infusion Withdrawal Programmable models also have RS 485 or optional RJ 11 ports for daisy chaining pumps and Digital I O for external control via an independent computer or device see page 13 for more information on connectivity Since 1901 Harvard Apparatus has been supporting bioresearch fluidics requirements with a key milestone being the introduction of the first commercial syringe pump for bioresearch in 1956 Since 1956 over 70 000 satisfied syringe pump users around the world have made Harvard Apparatus syringe pumps the worlds 1 choice The Pump 11 Elite Series is a family of accurate low flow syringe pumps designed for use in applications including mass spec calibration drug and nutritional studies reactor dosing electrospinning and more Easy to Use Interface The Pump 11 Elite Syringe Pumps are very easy to use with an LCD color touch screen and icon interface The Message Area of the touch screen is used to display helpful instructions for the currently displayed screen It is also used to display error or warning messages to indicate problem conditions in a Method or error conditions dur
46. n Only with Standard 2 Syringe Rack PC1 70 2223 PHYSIO 22 Infusion Only with 6 10 Multi Syringe Rack Note Anti Siphon syringe holder secures syringe plunger to pusher block to prevent unintended loss of fluid References Svoboda K et al Conformation and elasticity of the isolated red blood cell membrane skeleton Biophys J 1992 Vol 63 784 793 Jang IK Differential Sensitivity of Erythrocyte Rich and Platelet Rich Arterial Thrombi to Lysis With Recombinant Tissue Type Plasminogen Activator A Possible Explanation for Resistance to Coronary Thrombolysis Circulation 1989 Vol 79 920 928 Gao J et al Integrated Microfluidic System Enabling Protein Digestion Peptide Separa tion and Protein Identification Anal Chem 2001 Vol 73 2648 2655 Jayawickrama D A Wolters A M and Sweedler J V Mobile phase compensation to im prove NMR spectral properties during solvent gradients Analyst 2003 Vol 128 421 426 Hiramatsu M et al Reversion of muscarinic autoreceptor agonist induced acetyl choline decrease and learning impairment by dynorphin A 1 13 an endogenous k opioid receptor agonist British Journal of Pharmacology 1998 Vol 123 920 926 fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS Pump 33 Dual Syringe Pump KEY FEATURES Two independent pumping channels 2 syringes can run in the same or opposite direction simultaneously Program different flow rates for each syringe
47. on curves SYRINGES MIN MAX 50 ul 500 yl FLOW RATE Minimum 367 4 pl min Maximum 1 906 ml min INJECTOR HEAD ACTUATOR 20 3x 5 1 x 5 1 cm 8x2x2 in LxHxW Order Product PC1 70 3040 NanoCool Injector Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS Pump 11 Plus PC1 70 2209 Dual Syringe CE KEY FEATURES Q Small footprint Q RS 232 option for computer control Legendary reliability 2 year warranty APPLICATIONS Drug Nutritional Delivery Microfluidics Nanofludics Electrospinning Mass Spec Calibration Microdialysis Pump 11 Plus Harvard Apparatus Pump 11 Plus is a high performance smooth flow syringe pump at a basic syringe pump price Bright Display and Easy To Use Interface A two line 16 character vacuum fluorescent display and six membrane keypad make this a powerful easy to use syringe pump Only two entries are required to start pumping syringe Inside Diameter mm and flow rate The flow rate can be changed while the pump is running Two Modes of Operation Constant Flow Rate amp Volume Dispense The Pump 11 Plus will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode Versatile Select from two different models Infusion Only or Infusion Withdrawal Both models are available with either a single syringe or dual syringe configuration Harvard App
48. r communication for pump to pump USER 1 0 from PC communication CONNECTOR Specifications FOOTSWITCH INPUT Start and stop a pump USB SERIAL INPUT Control your pump with a computer BF locua ee ete d M NE PCE GCSE Connect multiple pumps together only or infusion withdrawal programmable daisy chain up to 99 pumps ACCURACY 0 5 Connect satellite pumps to the Master pump for REPRODUCIBILITY 40 0596 binary gradient system composition SYRINGE decus Connect multiple pumps together daisy chain Size single syringe 0 5 ul to 50 60 ml Set pump to infuse or withdraw Size dual syringe 0 5 ul to 10 ml Gontrol Input Trigger Input Connect an external device to start and stop FLOW RATE DOWD c a pump or Method Single Syringe 1 28 pl min to 88 28 ml min Signal another device to start and stop a pump or Method Dual Syringe 1 28 pl min to 25 99 ml min Connect an external LED or monitoring device to a pump DISPLAY 4 3 WQVGA TFT color display with touch screen pms ORS Order Product RS 485 IEEE 1394 6 position USB ee PC1 70 4500 Pump 11 Elite Infusion Only Single Syringe VO amp TTL 15 pin D Sub connector PC1 70 4501 Pump 11 Elite Infusion Only Dual Syringe Footswitch mini phono jack PC1 70 4504 Pump 11 Elite Infusion Withdrawal Programmable Single Syringe AVERAGE LINEAR FORCE 16 kg 35 Ibs 100 Force Selection PC1 70 4505 Pump 11 Elite Infusion Withdrawal Programmable Dual Syringe STEP RESOLUTION 0 069 um ustep INPUT POWE
49. rades must be factory installed PC161 0270 Continuous Flow Tubing Segment Remote Extension Cables Syringe Racks Replacement cables tor PHD 22 2000 remote syringe pumps The PHD 22 2000 is offered with a variety of syringe racks to meet including the PHD 22 2000 Hpsi see page 31 are available in oft your specific application Multi syringe racks provide multi channel and 30ft lengths These cables can also be used to increase or operation or serve as a large capacity reservoir decrease the distance between the syringe pumping mechanism and the control box e The push pull syringe pump holds 4 syringes 2 in each direction for syringe sizes 0 5 pl to 30 ml Order Product e The standard 2 syringe rack holds 2 syringes from 0 5 ul or 140 ml e The 4 x 140 multi rack holds four 60 ml glass or four 140 ml plastic syringes only PC1 72 0199 Remote Extension Cable 1 5 m 5 ft PC1 72 1405 Remote Extension Cable 9 1 m 30 ft e 6 10 multi rack will hold up to 10 syringes from 0 5 pl to 20 ml and up to 6 syringes from 30 ml to 60 ml e The microliter syringe rack independently holds 4 microliter syringes from 0 5 pl to 10 ml enabling syringes of different sizes to run simultaneously Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS PHD 22 2000 Hpsi and PHD 4400 Hpsi High Volume amp Pressure Programmable Syringe Pumps PHD 22 2000 Hpsi with four 200 ml Stainles
50. re dual syringe infusion withdrawal pumps They are available in three configurations standard push pull and high force These modules include a footswitch input USB RS 232 RS 485 and I O connectors They can be controlled via PC You can also start and stop a pump using the 15 pin user I O connector See Advanced Connectivity on page 18 for more information See page 20 for part numbers PHD ULTRA Remote Pumps The NEW PHD ULTRA Remote Pumps consist of a control unit and syringe pumping mechanism all connected via an RS 485 IEEE 1394 cable This pump includes a 30 foot RS 485 IEEE 1394 cable allowing the syringe pumping mechanism and the control box to be separated Every version of the PHD ULTRA is available in a remote model Use the remote pump in hazardous environments where the researcher is safer when distanced from the material being pumped or for applications where it is necessary to have your pump inside an incubator See page 20 for part numbers CE Harvard Apparatus phone 508 893 8999 tollfree US 800 272 2775 fax 508 429 5732 www harvardapparatus com 19 o lt 7 Z m U C lt U 6 U L U C F AJ gt gt Q lt Q I O OD Q o lt a I va 0 c U o0 o lt 7 Z m U C lt U 6 U IL U C F AJ gt gt Q lt Q O OD Q o lt va 0 3 U o Specifications TYPE ACCURACY REPRODUCIBILITY SYRINGES Type Si
51. ringe size detection Backup battery Unique clutch for one handed loading of syringes The MFRP10 Syringe Pump is a state of the art infusion pump originally designed for human use in intensive care units and anesthesia It is now sold for laboratory animal use only This pump can provide continuous or bolus infusion 32 Harvard Apparatus phone 508 893 8999 toll free US 800 272 2775 Specifications ACCURACY FLOW RATE COMPATIBLE SYRINGES Becton Dickinson Monoject Terumo B D Glass POWER RECHARGE TIME BATTERY CAPACITY USER SETTINGS ALARMS ALERTS STATUS ALERTS INFUSION MODES DIMENSIONS WEIGHT 3 excluding syringe variations 0 01 ml Rr 3 l hr dependent on syringe size 1 3 5 10 20 30 50 60 ml 1 3 6 12 20 35 60 140 mi 1 3 5 10 20 30 60 mi 1 2 5 5 10 mi AC power or DC internal rechargeable lt 8 hours Approximately 12 hours Audio Volume Alarm LOW NORM or HIGH Snooze Alarm Delay 2 to 60 minutes Plunger Force LOW NORM or HIGH Battery status Occlusion Bolus delivery Check clutch Syringe status Syringe size invalid Plunger Invalid number System malfunction Battery charging in use depleted Delivering Priming Standby mode Stop Program g kg min g kg hr mg kg min mg kg hr g min g hr mg min mg hr mu min mu hr u min u hr ml min ml hr dose volume delivery time 24 x 10 x 15 cm 9 5 x 4 0 x 5 9 in 2 2 kg 4 9 Ibs Order Product P
52. rvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 9 0 lt a Z I U C lt U 0 gt o O O ct O 2 V D Q et O 2 cC Q 10 SYRINGE PUMPS Application Selection Guide continued Microfluidics The PHD ULTRA series and Pump 11 Elite series pumps have the performance and flow stability required in micro and nanofluidics These pumps can be used to mix flow streams and or create multiple parallel flow streams Another option is the NanoLeader pressure pump that can push and or pull fluids using positive or negative air pressure creating the smoothest flow possible Pump 11 Elite 10 ml Syringe 216 66yl min 11 Elite Flow Rate yl min 600 Time Sec References SENSORS AND ACTUATORS B CHEMICAL Volume 140 Issue 1 18 June 2009 Pages 295 303 PNAS March 4 2008 vol 105 no 9 3191 3196 BIOMEDICAL MICRODEVICES Volume 10 Number 6 795 805 ANAL CHEM 2009 81 8 pp 2854 2859 Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 Nano Micro Liquid Chromatography The advanced programming capabilities of the PHD ULTRA and the Pump 11 Elite Syringe Pumps allow for the easy generation of binary or ternany gradients at flow rates ranging from 100 nl min to 1 0 ml min at pressures up to 7 500 PSI Depending on the number of syringes used up to one liter of mobile phase may be delivered from e
53. s Steel Syringes syringes not included PHD 4400 Hpsi Programmable CC KEY FEATURES KEY FEATURES Pump up to 800 ml with four 200 ml syringes Accurate and smooth flow High accuracy and reproducibility Easy to use interface Rugged construction for a lifetime of service RS 232 serial port for computer control Accepts only Harvard Apparatus stainless steel syringes Q Delivers over 200lbs of pumping force across a wide flow rate range Q Legendary reliability 2 year warranty Q Legendary reliability 2 year warranty APPLICATIONS APPLICATIONS Q High Pressure Injections Drug Delivery Q Pumping Highly Corrosive Fluids Q Injecting into High Pressure Reaction Vessels Specifications Specifications High Pressure Injections Drug Delivery Q Q Pumping Highly Corrosive Fluids Q Injecting into High Pressure Reaction Vessels Bre ICON Sar OE Synge TYPE Microprocessor single syringe infusion withdrawal programmable infusion withdrawal programmable ACCURACY 0 5 ACCURACY 0 35 REPRODUCIBILITY 0 05 REPRODUCIBILITY 0 05 SYRINGES MIN MAX Holds 20 to 200 ml stainless steel syringes SYRINGE FLOW RATE Type Stainless steel Minimum 1 5 ul hr Size Minimum 2 5 ml Maximum 112 ml min Size Maximum 100 ml RS 232 RJ11 4 conductor FLOW RATE TTL 9 pin D Sub Connector Minimum 0 0076 ul min AVERAGE LINEAR FORCE 197 kg 433 Ibs imum 182 40 ml min STEP RESOLUTION 0 0397 um step RS 232 RJ 11 4
54. s a single syringe infusion withdrawal programmable syringe pump This pump allows you to create save and run simple to complex Methods without a PC The flow rate range is 3 66 pl min to 3 818 ml min with 11 Ibs of adjustable force across the entire flow rate range The PHD ULTRA Nanomite has a footswitch input RS 232 and USB serial ports for computer control RS 485 ports for daisy chaining pumps and Digital I O for external control via an independent computer or device There is also an option for daisy chaining pumps through the RS 232 RJ 11 ports This option must be ordered at the time the pump is ordered See page 18 for more information on connectivity This pump consists of a control unit an injection unit a 6 foot cable to connect the two units and a footswitch For more information on the PHD ULTRA Nanomite including features and programming see the PHD ULTRA on page 16 Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 Infuse withdraw SGE 1 03 mm 50 00 ul Target 50 ul Infuse 158 984 ul min Withdraw 158 984 ul min Flow Direction Hand Held HARVARD APPARATUS 08 18 09 1 29 18 PM Ready to infuse Press the green Run button to start the infusion or press the back button to return to the previous screen L1 0 000 ul Time Elapsed lime Remaining Total Inf Volume Force Level TYPE ACCURACY REPRODUCIBILITY SYRINGES Type Size Minimum Size Maximum FLOW
55. s the PHD ULTRA is the new standard for syringe pumps 1 NEW Patent pending mechanical drive mechanism and syringe holding mechanics to achieve the highest performance of any syringe pump 2 NEW EZ PRO Software and user interface allow easy programming of Methods from simple to complex all without the use of a PC Preprogrammed Methods for simple to complex operations that allow you to be up and running with the touch of a button LCD high resolution color touch screen for powerful functionality yet very easy to use 3 NEW Levels of Versatility a Configurations Standard push pull remote high pressure multi racks b Connectivity For USB or RS 232 computer control RS 485 or optional RJ 11 for daisy chain control multiple pumps c Orientation Horizontal or vertical orientation to optimize bench space or to minimize tubing Since 1901 Harvard Apparatus has been supporting bioresearch fluidics requirements beginning with the introduction of the first commercial syringe pump for bioresearch in 1956 Since 1956 over 70 000 satisfied syringe pump users around the world have made Harvard Apparatus syringe pumps the world s 1 choice The PHD ULTRA Syringe Pump series is a family of high accuracy easy to use rugged pumps designed for versatile applications including mass spectrometry calibration drug and nutritional studies microdialysis dispensing chromatography LC HPLC and more fax 508 429 5732 w
56. s to create and store up to two user defined Methods of 50 steps each on the pump Accessories A full range of accessories are compatible with the Pump 11 Elite including syringe heaters in line heaters and coolers nanofluidic circuits connectors tubing syringes and more Program Description To operate the Pump 11 Elite the user defines all the required parameters for infusing or withdrawing liquids through a Method This may be a Quick Start Method Pre Programmed or User Defined Method The basic operation is a simple 3 step procedure 1 Select a method 2 Enter operating parameters 3 Preview or Run your method Quick Start Methods are simple infusions withdrawals or a combination depending upon the model Custom user defined Methods can be created when more advanced programming is required The setup for a custom Method is easy using the standard profiles found on all Infusion Withdrawal Programmable Elite Models The list of available profiles is Gradient binary By programming custom user defined Methods into the pump multi user errors are reduced Easily transfer Methods to other pumps and or download Methods from a PC Forget having to duplicate Method development efforts for each new pump added to your system HARVARD APPARATUS Ready to infuse Infuse Only BD plastic 11 99 mm 5 00 ml Target 5 ml Infuse 5 ml min Flow Direction Time Elapsed 00 00 00 Time Remaining 00 01 00 Total In
57. sories PC1 70 4000 PC1 70 4001 PC1 70 4020 PC1 70 4002 PC1 70 4003 PC1 70 4004 PC1 70 4005 PC1 70 4006 PC1 72 8340 PC1 70 2215 PC1 55 7002 PC1 55 7008 PC1 55 7760 PC1 72 2478 PC1 55 8000 fax 508 429 5732 RS 485 Cable for Pump to Pump Communication 0 5 m 1 6 ft RS 485 Cable for Pump to Pump Communication 2 m 6 6 ft RS 485 Extension Cable 9 1 m 30 ft USB Cable for PC to Pump Communication 2 m 6 6 ft USB Cable for PC to Pump Communication 5 m 16 4 ft RS 232 Cable for PC to Pump Communication 9 pin D sub 2 m 6 6 ft Adapter PHD Digital I O Adapter D sub 15 to Term Bik Adapter USB to Serial Footswitch with Phono Plug Auto Fill Valve Box Normal Pressure 30 psi Auto Fill Valve Box High Pressure 200 psi Cable Assy Daisy chain Legacy RS 232 RJ 11 0 6 m 2 ft Cable Assy Daisy chain Legacy RS 232 RJ 11 2 1 m 7 ft Adapter for 25 ml 50 ml 100 ml Hamilton GasTight Syringes www harvardapparatus com SYRINGE PUMPS NEW Mlixture Dose Delivery System PC1 70 4102 PHD ULTRA Gradient System with Stand CE KEY FEATURES Easily run binary or ternary gradients without a PC Combine multiple flow streams into one common output Q No stopping experiment to fill syringes with different mixtures APPLICATIONS Serial Dilutions of reaction dosing solutions Serial Dilutions for drug infusion experiments Serial Dilutions for nutritional infusion experimen
58. stry 2008 Vol 54 5 891 900 Choi S and Park J Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array Lab on a Chip 2005 Vol 5 1161 1167 Tan YC Li Ho Y and Phillip A Microfluidic sorting of droplets by size Microfluid Nanofluid 2008 Vol 4 343 348 Cucchiaro G Chaijale N and Commons K G The locus coeruleus nucleus as a site of action of the antinociceptive and behavioral effects of the nicotinic receptor agonist epibatidine Neuropharmacology 2006 Vol 50 769 776 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 25 0 lt a Z 0 m U C lt U 0 U 3 o N N Q 5 Q U lt 0 O N N lt c ap 9 D 0 lt a 5 va D y c 3 o 0 26 SYRINGE PUMPS Pump 22 and Physio 22 Multiple Syringe Pumps CE KEY FEATURES Smooth and accurate flow Easy to use Versatile with wide flow rate range Multi syringe racks for large reservoir capacity Legendary reliability 2 year warranty APPLICATIONS Drug Nutritional Delivery Q Fluid Sampling Q Mass Spectrometry Pump 22 The Harvard 22 Syringe Pump is the pump that set the industry standard It is the world s most popular syringe pump Harvard Apparatus long standing tradition of providing rugged and reliable products is the foundation upon which this pump was bullt e The standard 2 syringe rack holds 2 syringes from 0 5 pl to 140 ml e The4x 14
59. t va 0 j U o0 SYRINGE PUMPS NEW PHD ULTRA Advanced Syringe Pumps MID APPARA UN TI HARVARD APPARATUS CE KEY FEATURES New patent pending drive mechanism for unmatched smooth flow accuracy and precision For operation at pl min to ml min flow rates Easily program simple to complex methods without a PC Alphanumeric keypad for easy Method naming and recall Real and relative time clocks Intuitive touch screen and icon interface Vertical or horizontal orientation Adjustable linear force to 75 Ibs Multi syringe racks for multi channel operation or large capacity reservoir Legendary reliability 2 year warranty APPLICATIONS 16 Microfluidics Drug Nutritional Delivery Electrospinning Reaction Chamber Addition Mass Spec Calibration Feeding Cells Low Pressure Chromatography Continuous Flow Flow Programming Gradients Composition Step Changes Large Flow Deliveries I O Interactive Experiments Harvard Apparatus phone 508 893 8999 TERRAIN toll free U s 800 272 2775 X Fo I I ima i HARVARD PHD ULTRA The PHD ULTRA is the solution for your most demanding fluidics applications This pump represents the latest technology in syringe pumps and was developed utilizing the feedback of the world s largest population of syringe pump users The PHD ULTRA will change the way you think about syringe pumps There are three major reason
60. table 16 kg 35 Ibs 21 kg 47 Ibs adjustable RS 232 139 232 USB RS 232 USB USB Yes Yes Yes Yes Yes 28 x22 2 x 14 cm In xu 10 16x30 5x21 6 cm 226x 17 78x15 cm 22 6x17 8x15 cm Re 5 55m 6 3x9x 11 in 4x12x8 5 in 9x7x6 in 9x7x6 in 4 5 kg 10 Ibs 4 5 kg 10 Ibs 4 5 kg 10 Ibs 2 1 kg 4 6 Ibs 2 1 kg 4 61 Ibs 28 29 16 11 14 Please contact Technical Support for assistance Depends upon the Syringe Rack 4 x 140 Syringe Rack 6 x 10 Syringe Rack Microliter Syringe Rack Holds four 60 ml or 140 ml plastic syringes only Holds six 30 to 60 ml syringes or ten 0 5 ul to 20 ml syringes Holds four 0 5 ul to 10 ml syringes ow RFI Radio Frequency Interference Pump available Push Pull pump can hold syringes up to 140 ml if full stroke is not required Larger syringes will not fully infuse or withdraw Max of 50 ml syringe if full stroke is required Harvard Apparatus phone 508 893 8999 toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com SYRINGE PUMPS Choosing the Right Pump for Your Application amp Budget continued SYRINGE PUMP QUESTIONS CONTINUED What is the viscosity of the liquid you are pumping See pump reference page 101 What are the pressure requirements of your experiment See pump reference pages 101 102 Does the pump need to continuously infuse over a 24 hour period of time Does the pump need to be programmable Does the pump need to be controlled with a computer
61. ts Serial Dilutions for mixing polymers in electrospinning Chromatography FIA systems The PHD ULTRA Gradient Systems allows you to quickly and easily run binary or ternary mixtures automatically without a PC The Method is totally programmed from a master controller pump so it is easy to program The intuitive program interface makes simple to complex mixing easy The Gradient Profile is used to combine multiple flow streams from different pumps into a common output stream The flow can be smooth and continuous or stepped No stopping the experiment mixing different volume mixtures and placing new syringes into the pumps Push one button and it all happens automatically Binary and ternary gradient systems are available with or without a stand Individual Satellite units are also available for purchase separately For more information on the PHD ULTRA Syringe Pumps see page 16 For more information on the PHD ULTRA Satellite units see page 19 Harvard Apparatus phone 508 893 8999 toll free U s 800 272 2775 Flow Rate TYPE ACCURACY REPRODUCIBILITY SYRINGES Type Size Minimum Size Maximum FLOW RATE Minimum Maximum DISPLAY CONNECTORS RS 232 RS 485 USB 1 0 amp TTL Footswitch LINEAR FORCE STEP RESOLUTION VOLTAGE RANGE DIMENSIONS H x W x D WEIGHT CERTIFICATIONS Order PC1 70 4101 PC1 70 4102 PC1 70 4106 PC1 70 4107 Specifications Stepped Continuous and Ramped gra
62. ww harvardapparatus com SYRINGE PUMPS NEW PHD ULTRA Advanced Syringe Pumps continued Q w E R HIE I TJ i JLI Enter your settings to the left and then press the green Run or Run Preview button when finished Make sure to set a Select baud rate and ten the Enter button guen cred mark to accept or press the 318 30 3 173 0 T HR HERE L i l Ji J IL i if AI PO GE E AIR EET HEFT 00123 Space Highest Accuracy and Precision The PHD ULTRA syringe pump family has a new patent pending fluidics drive mechanism which assures ease of use and high performance for the smoothest most accurate flow rates of any syringe pump Flow rates of 1 56 pl min to 215 8 ml min are accurate within 0 25 and reproducible within 0 05 A microprocessor controlled small step angle stepper motor drives a lead screw and pusher block Advanced micro stepping techniques are employed to further reduce the step angle to eliminate flow pulsation Maximum Experimental Versatility This pump can be oriented vertically or horizontally for optimum experimental connectivity This pump comes standard to hold 2 syringes but can be purchased with 3 other syringe racks 6 10 syringe rack 4 x 140ml syringe rack and 4 x microliter syringe rack Syringe racks can be ordered separately Multi syringe racks provide multi channel operation or serve as a large capacity reservoir e The standard 2 syringe rack holds 2 syr
63. yringe Pump PC1 61 0270 Continuous Flow Tubing Segment CC Specifications TYPE ACCURACY REPRODUCIBILITY SYRINGE Type Size Minimum Size Maximum FLOW RATE Minimum Maximum NON VOLATILE MEMORY CONNECTORS RS 232 TTL AVERAGE LINEAR FORCE Standard High Pressure POWER VOLTAGE RANGE CABLE LENGTH DIMENSIONS H x Wx D WEIGHT Microprocessor multiple syringe infusion only infusion withdrawal or infusion withdrawal programmable 0 35 0 05 Plastic glass or stainless steel 0 5 ul 140 ml 30 ml for push pull models 0 0001 ul hr 220 82 ml min Stores all settings RJ11 4 conductor 9 pin D Sub Connector 50 Ibs 66 Ibs 65 W 0 5 A fuse Universal input 100 240 VAC 50 60 Hz 9 1 m 30 ft for remote models only 15 9 x 22 8 x 27 9 cm 6 3 x 9 x 11 in 4 5 kg 10 Ib Push Pull pump can hold syringes up to 140 ml if full stroke is not required Larger syringes will not fully infuse or withdraw References Mazutis L et al Droplet Based Microfluidic Systems for High Throughput Single DNA Molecule Isothermal Amplification and Analysis Anal Chem 2009 Vol 81 4813 4821 Yeoma J et al Microfabrication and characterization of a silicon based millimeter scale PEM fuel cell operating with hydrogen methanol or formic acid Sensors and Ac tuators 2005 Vol B107 882 891 Guillot P et al Towards a continuous microfluidic rheometer Microfluid Nanofluid 2008 Vol
64. ze Minimum Size Maximum FLOW RATE Minimum Maximum DISPLAY CONNECTORS RS 232 RS 485 USB 1 0 amp TTL Footswitch LINEAR FORCE STEP RESOLUTION VOLTAGE RANGE DIMENSIONS H x W x D WEIGHT REGULATORY CERTIFICATIONS Product Order Order Order Order PHD ULTRA x Syringe Pump Standard Stand Alone Remote Satellite Module PHD ULTRA Infusion Only PHD ULTRA Infusion Withdrawal PHD ULTRA Infusion Withdrawal Programmable PHD ULTRA Push Pull PHD ULTRA Push Pull Infusion Withdrawl PHD ULTRA Push Pull Infusion Withdrawl Programmable PHD ULTRA High Pressure PHD ULTRA 4400 Pump W Programmable PHD ULTRA Hpsi Remote Pump I W Programmable PHD ULTRA Hpsi Remote Pump I W Programmable with 10 x 140 Rack Stand Alone Stand Alone SYRINGE PUMPS NEW PHD ULTRA Advanced Syringe Pumps continued Microprocessor multiple syringe infusion only infusion withdrawal or infusion withdrawal programmable 0 25 0 05 Plastic glass or stainless steel 0 5 ul 140 ml 1 56 pl min 215 8 ml min 4 3 WQVGA TFT color display with touchscreen 9 pin D Sub Connector 6 position IEEE 1394 Type B 15 pin D Sub connector Phono jack 34 kg 75 Ibs 100 force selection 0 082 um step Universal input 100 240 VAC 50 60 Hz 10 16 x 30 48 x 21 59 cm 4 x 12 x 8 5 in 4 5 kg 10 Ibs CE ETL UL CSA WEEE EU RoHS amp CB Scheme PC1 70 3005 PC1 70 330

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