- User Manual
Contents
1. Adapter 1 4 28 Female 1 4 28 Female Nylon P N 187210 only for the SPOT II Union PEEK 10 32M 1 4 28F P N 737664 Internal reducer 1 16 1 8 P N 229980 only for semi prep and prep configuration on puriFlash 4100 puriFlash 4250 250 puriFlash 4250 40 Installation procedure 12 Funnel rack set 1 unit or Funnel rack set 4 units 13 Safety solvent caps kit 4 units 14 Safety waste cap with container 5L filter or Safety waste cap with container 20L filter 15 Drainage System of the tray collector 16 6 ways electric valve module 6 ways electric valve with loop 2mL loop by default Tubing Power cable model according to the destination country Serial communication cable 1 x Sub D9 male female Distribution power cable 1 x main voltage supply Installation procedure 17 Semi Prep and Prep adaptation kit 18 2 columns selection switching valve Switching valve ETFE tubing Installation procedure 19 Backflush valve Backflush valve ETFE tubing Installation procedure 20 2 columns selection switching valve Backflush valve 21 6 ways electric w loop 2mL loop by default Backflush valve module 2 valves ETFE tubing Installation procedure 22 6 ways electric w loop 2mL loop by default 2 columns selection switching valve module 2 valves ETFE tubing Installation procedure 23 6 ways electric w loop 2mL loop by default2. 2 columns selection switching valve module Backflush valve module valves ETFE tubing Installation procedure 24 Air purge 25 RI IOTA2 1V Output cable Power cable ETFE Tubing 1 16 ID 1mm RI outlet fitting 10 32 Male P N 780770 UV inlet fitting and ferrule for 1 16 tubing P N 167155 and AB8790 length 70cm Adapter 1 4 28 Female 1 4V 28 Female Nylon P N 187210 only for the puriFlash SPOT II User s Manuel Installation procedure 26 Mass spectrometer interface 27 CarouXel Power cable RS 232 cable Installation procedure 28 Autosampler Power cable RS 232 cable User s manuel Installation procedure 29 Ballasting kit for 1 8 tubing 5 units 30 Racks set 13x100mm P N PF4370 16x150mm P N PF4380 18x150mm P N PF4390 21x150mm P N PF4410 25x150mm P N PF4420 28x180mm P N PF4430 29 5x200mm P N PF4440 Plateform rack for bottles P N LV5200 31 2 fraction collector coupled Fraction collector General power cable Serial communication cable Sub 09 male female Tubing with 2 fitings and 2 ferrules 2 valves 8 a ETFE tubing I 3m 1 8 ID 1 6mm Installation procedure EE D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUTI LASH 33 4 70 03 73 01 4 gt Operation a 4 1 Control the system
3. When all the installations and connections are made switch on the PURIFLASH 220 After few seconds and the initialisations of different instruments the control software Interchim Software automatically starts Note the good starting no message Communication Error is necessary for the correct working of the system TH HERBE EN EIRISEaT Hwan HSH MOD M RI IT et 4 Hoban DO m S nd ui Li aun P n il e D VR TE rr keere u Sr mom mnm o Due me wn o m RO Pop D n M n cgo P zb ud mem D m SU nm CDS en DOS pes RE em WE mn Once the user interface opened several graphic elements allow to see in real time the state of the instruments and the evolution of the working parameters The application has been developped with the aim of being convivial and intuitive Refer to the Instruction Manual supplied for more explanations on the software use Use the stylus on the tactile screen to create methods in the software and control the system Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr 4 2 Make a liquid injection Injection Mode Equilibration S s Answer Yes to the question of the software The method starts D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 w
4. two sizes of tube are used 1 8 ID 2 4mm for suction 1 8 ID 1 6mm for others D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com RE PUTI LesH 120 33 4 70 03 73 0 Follow the instructions below to correctly install the tubing Suction Provide yourself with the four Suction tubes I 1 5m 1 8 ID 2 4mm supplied they are more brittle than others so take care not to bend them For the tube A take the extremity where there is a fitting with a long spring and put the ferrule in the PEEK body of the suction valve Screw the PEEK nut tighten it hand tight and position properly the long spring Renew the same operation for B C and D as indicated on the picture Outlet e wa de d Gradient valve body PEEK nut ETFE plug for gradient valve To ensure the pumping continuity of the solvents and avoid the air entry in the hydraulic circuit the others extremities can be equipped with ballasts in option P N DZ7360 It consists in fact to put a fitting nut set ferrule and screw it in a stainless steel piece which serve of weight to maintain the tubes at the bottom of the containers PEEK nut Ballast Position the solvents containers on the solvent tray for instance and put the ballasted tubes into them Gradient valve The gradient valve to Tee Pump inlet tube is already connected into the p
5. 2 Removing a pump head 6 3 Replacing a seal 6 4 Cleaning replacing a check valve 6 5 Replacing the detector deuterium lamp 6 6 Cleaning the detector flow cell 7 gt Preventive measures Upkeep schedule page A 30 8 gt Warranty page A 31 8 1 Generalities 8 2 Limited warranty of consumable parts 9 gt List of spare parts Blow up views page A 34 10 Specifications page A 40 Appendix Rack sets available page A 41 gt Appendix 2 Service report age A 42 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual 1 gt General description The PURIFLASH 20 system is a modern instrument designed for Flash chromatography which allows to withdraw mobile phases from four containers of solvents form a gradient inject liquid or solid samples and provide the solution to a chromatography column detector measures the absorbance and gives a signal called chromatogram which is visualized with the built in computer and the control software INTERCHIM Software installed Then fractions can be collected with the fraction collector The characteristics of a PURI En 7720 are v Flow rate Up to 200ml min v Pressure Max 30 bar 435 psi v Gradient Quaternary v Column size From F4 to F1600 format according to column trademark v UV Detection Spectrophotom
6. FRANCE Takaa ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com User Manual The computer and its touch screen The system is controlled by the central processing unit via a touch screen which replace the keyboard and the mouse The operator uses the control software and can create methods to run the instrument Touch screen USB Ports Power light This module contains the main ON OFF switch and centralizes the connection for data and power supply to the other modules Four USB connectors are also available 1 in front of the system under the screen and 3 to the left hand side cable maximum length 2m The external connections Some others external connectors allow to plug in additional devices like second detector or options The maximum length of all external cables connected to the system is 2 metres LAN plug Local Area Network Serial communication port Others external connectors Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Dui ella re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr Optional Level sensors plugs External controls Analogic signal inputs 2 Packing list Standard system PUR Leen 7720 Item PURIFLASH 7720 system composed of Pump module Column holder module Detector module Fraction collector module Computer amp power supply module
7. approved professionals and within the technical characteristics compatible with the functions defined in the user manual Terms and conditions of the guarantee v The device is only guaranteed if installed by a service provider approved by Interchim v The guarantee covers the supply of parts found defective by Interchim free of charge as a minimum within the limits of the parts listed in part 8 2 v The device must have been used in normal operating conditions and in accordance with the instructions v The device must have been used with the consumables recommended by Interchim v Exhaustive list of original parts covered by the guarantee part 8 2 Il Guarantee exclusions v The guarantee will not cover devices installed by the customer or by a service provider not approved by Interchim v The guarantee will not cover equipment used in a manner which is non compliant with the provisions in the instructions v The guarantee will not cover equipment subject to interventions repairs or modifications by personnel without Interchim approval v The guarantee will not cover parts modified or changed by the customer or the service provider without Interchim s approval parts damaged by this modification parts not recommended by Interchim parts subject to wear and consumables v The guarantee will not cover any parts not listed in part 8 2 v The guarantee will not cover electrical and or electronic and or IT incident
8. steel fitting set 1 8 ID 1 6mm 1 50m Outlet tubing 1 8 ID 1 6mm 1 50m PEEK 74 28 long nut for 1 8 tubing x 3 ETFE ferrule for 1 8 tubing x 3 CD for TRP converter drivers installation External control cable USB cable Power cable model according to the destination country Hardware Installation procedure 10 2 Acquisition channel Module USB 1408FS USB cable USB key for drivers installation Installation procedure 11 FLASH ELSD FLASH ELSD 1V Output cable Power cable Splitter box with micro needle option Kai 08 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual ETFE Tubing 1 16 ID 0 25mm Splitter Outlet ELSD Inlet length 30cm fitting 10 32 Male P N 780771 connected to Splitter box ETFE Tubing 1 8 ID 1 6mm Splitter Outlet UV Inlet length 72cm Adapter 74 28F 10 32 Male P N 737664 spring 20 cm P N PFS800 connected to Splitter box ETFE Tubing 1 8 ID 1 6mm Column Outlet Splitter Inlet length 72cm Adapter Ya 28 F 10 32 Male P N 737664 connected to Splitter box Manometer P N FJ6720 option 2 red or white tubing to connect the gas 6 mm OD 2 meter 1 meter and fitting 1 PEEK fitting 10 32 Male fitting Inlet ELSD P N 780771
9. the electrical supply 3 Remove the plastic cover to gain access to the pump heads 4 Disconnect the Suction and Delivery tubing and fittings 5 Remove the two screws Allen wrench 3mm Carefully unscrew alternating from one to the other 6 Carefully remove the pump head The piston and the seal are now visible D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com 33 4 70 03 73 01 6 3 Replacing a seal There are in all four seals one of every head and one of every cleaning disc You can Piston seal l exchange them by removing the pump heads and cleaning disc as explained previously 1 Remove the seal using a small screwdriver Be sure to avoid scoring any part of the pump head and the cleaning disc as a scratch will prevent proper sealing If the seat is scored it is necessary to replace the head or the cleaning disc and these are not under warranty 2 Thoroughly clean the seal seat with alcohol to remove possible seal particles and plunge the new seal in propanol 2 for lubrication 3 Place the new seal into the pump head or the cleaning disc and provide yourself with a special tool sold with the first set of seals P N PFS040 4 Position the part A on the head and slip the part B into A Then hit gently with the fist on the part B Tool 2 Parts 5 Remove the tool and note that the seal is
10. well placed in the pump head or in the cleaning disc 6 Finally replace the cleaning disc and the head on the piston and put its two fixing screws with care alternating from one to the other 7 Connect the Suction and Delivery tubing and fittings Note The life time of the seals is dependent on the flow rate pressure type of liquids used and temperature but mostly on the cleanliness of the mobile phase and sample The presence of micro particles will cause accelerated wear and tear of the seals Similarly any dried buffer particles on the piston will damage the seal 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Takaa ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 6 4 Cleaning replacing a check valve Abnormal pressure fluctuation or the observation of pump noise on the data system Check valve is usually due to check valve problems The noise is regular in nature and is due to pressure and flow variation Pump accuracy is also compromised Details on check valve repairs are given below There are four check valves two per head one for each inlet model 10mm P N PFS930 and one four each outlet model 8mm P N PFS920 If they become dirty they will not open and close correctly for a long time 1 Purge the pump head with a suitable solvent or alcohol 1 propanol or 2 propanol 2 Switch off the module and disconnect it from the ele
11. x Height x Depth without connection v Weight 60kg v Temperature 5 to 40 C v Others Indoor use Altitude up to 2000m Pollution degree 2 Noise level lt 35dB Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual gt Appendix 1 Rack sets available As mentioned in the chapter 3 4 Prepare the fractions collection several racks are usable with the fraction collector Here is a recapitulative list indicating their designation the features of tubes used and the total capacity of the rack set Short racks a rack set includes 4 racks Designation Tubes size Tubes volume Tubes by rack Tubes by rack set Total volume 18 x 150mm 18 x 180mm PF4370 13 x 100mm PF4380 16 x 150mm PF4410 21 x 150mm PF4420 25 x 150mm PF4430 28 x 150mm PF4440 29 5 x 200mm PF4390 Jure E C AR a PURFLASH 430 E I 200 ml min up to 30 bar i CRM Gg m I gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi description M r o Sh ME Ville AC E NR EE EE EEEE eA E E E TE E E P N and description of defective spare e Any further action required Leia ce senos Una rax ret thus seras seeds cette ess s vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenede
12. 530 An optional pre column holder is available to use greater dry load P N GO9640 Injection Mode Equilibration Wait for Ini In the panel Settings of the method edited select Equilibration in the section Injection Mode According to the type of column chosen a time and a flow rate are written by default they depend on its parameters but you can modified them if need be eg After having launched the method RUN the equilibration Make your injection and then Yes Gees sx phase starts That allows to condition the column with the solvents used At the end of the time or at a click on START the pump stops and the following dialog box appears on the screen m n D Unlock the black ring of the fitting Adapter PEEK Luer lock male to 28 Male from the Quick stop luer check valve Put the dry load with sample on the Quick stop luer check valve BECH _4 Screw the fitting Adapter Answer Yes to the question PEEK Luer lock male to of the software The method starts a 28 Male Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual 4 4 Make a loop injection optional To inject through a loop whose the volume is predefined from 1 to 40mL or more the system requires the option Loop injection 6 way valve kit Eq
13. Ca m E SW 4 TE x XE Se S E pr EN F E I j ef F 4 f Di I P3 T 1 go a ps Fu T i WW I 5 H g Wars il L ag WW WR E it INTERFINE CHEMICALS ANALYTICAL SCIENCES BIOCHROMATOGRAPHY BIOSCIENCE INNOVATION PUR LASH 330 User VEEL ITM 2012 10 31 Ultra Performance Flash Purification Enter the UPFP Concept Lab Scale amp Routine v Flash columns up to F300 format v Prep LC columns up to 80mm id 15um silica PURFLASH 430 200 ml min up to 30 bar INTERFINE CHEMICALS e 211 bis avenue JF Kennedy Ke nte e h m BP 1140 03100 Montlucon Hotline 33 4 70 03 73 01 interfine interchim fr Fax 33 4 70 03 82 60 ET wt 33 4 70 03 73 01 Summary 1 gt General description page A 3 2 gt Packing list page A 7 3 gt Installation page A 8 3 1 Assembling the system 3 2 Install the tubing and prepare the containers 3 3 Set up the column and the dry load on the column holder 3 4 Prepare the fractions collection 3 5 Position the stylus 3 6 Make the connections electrical and data transmission 3 7 Options and accessories 4 gt Operation page A 20 4 1 Control the system 4 2 Make a liquid injection 4 3 Make a solid injection 4 4 Make a loop injection optional o gt Safety regulations page A 24 6 gt Maintenance page A 25 6 1 Introduction 6
14. ES Signature gt Service report lt O Installation End of warranty visit O Warranty O Repair O Maintenance O Out of warranty ea er le ME NE E P Ville lei 1 ICS CO E painterchim 211 bis avenue J F Kennedy Tel 33 4 70 03 88 55 BP 1140 03100 Montlucon Fax 33 4 70 03 82 60 Hotline 33 4 70 03 73 01 E mail interfine interchim fr Not contractual pictures
15. Gradient valve CPU2010 02480 Collection 3 way valve PFS910 gt Terms of use Normal tightening of the fittings Use of clean solvents and clean containers Follow up of the preventive maintenance actions gt Damages not covered Damaged thread or crushed internal part due to unsuitable tightening Blocked passage due to uncleanliness of installation or unsuitable mobile phase Pump Check valve 8mm PFS920 6 months Check valve 10mm PFS930 6 months Piston 8 5 x 65 kit PFS080 1 year Seal 8 5mm PFS940 6 months gt Terms of use Use of clean solvents and clean containers Follow up of the preventive maintenance Respect precisely the maintenance procedures Damages not covered Damaged piece due to unsuitable use assembly or replacement Blocked passage due to uncleanliness of installation or unsuitable mobile phase Detector Flow cell 0 3mm PFS960 1 year 6 months or 1000 hours Deuterium lamp PFS970 gt Terms of use Use of clean solvents and clean containers Respect precisely the maintenance procedures gt Damages not covered Damaged piece due to wrong use or replacement or inappropriate solvent pumping Blocked passage due to uncleanliness of installation or unsuitable mobile phase D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC T l 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com PUTI LesH 33 4 70 03 73 01 9 gt Li
16. Installation procedure 3 Pre column holder Guidance rail Stainless steel bulkhead union 90 holder Adapter Stainless steel Luer lock male to 74 28 male Stainless steel Luer female to 14 28 male 1 8 tubing ID 1 6mm 3m Spring for tube 15cm PEEK 74 28 long nut for 1 8 tubing x 2 ETFE ferrule for 1 8 tubing x 2 Hardware Installation procedure 4 6 ways valve w loop injection kit Low pressure 6 way valve Mouting Bracket 1 8 tubing ID 1 6mm 28cm Spring for tube 20cm Spring for tube bcm PEEK 74 28 short nut for 1 8 tubing for GV1690 x 1 PEEK 74 28 long nut for 1 8 tubing for GV1690 x 1 TEFZEL ferrule with Stainless steel lock ring for 1 8 tubing x 2 Adapter ETFE luer lock Female to 74 28 Male 1 16 tubing with fitting ferrule 1 m50 Lopp 1mL E Hardware NN Installation procedure 33 4 70 03 73 01 5 Bar code reader Reader with USB connection Bracke Hardware Installation procedure 6 Enhancement tube device for 13 x 100mm tubes 7 Video camera TLC plates holder Webcam with USB connection Bracket CD for Webcam drivers installation TLC plates holder Hardware Installation procedure 8 Fume enclosure Side panel Transparent door 5 screws Collar Magnetic flap Hardware Installation procedure 9 Quad system Module for 4 additional columns installation Inlet tubing with stainless
17. TFE 1m 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule spring pipe Valves Quaternary Gradient valve CPU 2010 102480 Collection 3 way valve PFS910 Electronic boards Electronic board CPU2010 102950 Electronic board Collector PFS500 PFS810 102650 102660 102670 PFS860 PFS870 PFS880 System Flex for collector connections PFS490 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Part Number N Check valve 8mm PFS920 4 Check valve housing 8mm PFS020 6 Check valve 10mm PFS930 3 Check valve housing 10mm PFS010 2 Seal 8 5mm PFS940 5 Head PFS030 1 Tool for insertion of seals D 8 5mm PFS040 i T T 2 cond Piston 8 5 x 65 kit if cleaning disc PFS080 9 Cleaning discs x2 102970 14 Head support bearing PFS050 11 12 Head support reinforcement PFS060 2 Camshaft 2 Cams PFS110 8 Compression spring PFS100 7 Pump opto sensor cable PFS180 6 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com POGUE L SH Item Pump suite Opto sensor disc Pull piston Camshaft holder parts Balancer kit Balancer cable Belt Pulley big camshaft Pulley small motor Motor Black Motor holder Motor d
18. Y Tubing with fittings ETFE OD 1 8 Suction 1 50m ID 2 4mm x4 with 20cm spring Gradient valve outlet to Tee Pump inlet 28cm ID 2 4mm present on the system Pump outlet to Column Holder inlet 60cm ID 1 6mm Column holder outlet to Detector inlet 70cm ID 1 6mm Detector outlet to Fraction collector inlet 30cm ID 1 6mm Waste outlet 1 50m ID 1 6mm Pressure test OQ 50cm 1 16 ID 0 5mm v Rack set PF4390 for 18mm diameter tubes 4 racks v Solvent Tray with a drainage system v Serial communication cables 2 x Sub D9 male female 1 x Sub D9 female female v Distribution power cables 2 x main voltage supply 1 x 24V v Fittings for cleaning discs connections 2 long nuts 2 ferrules v Binding column v General power cable model according to the destination country v USB key including the file of installation exe of the freeware Interchim Software Demo v Stylus amp Keyboard v Columns starting kit 2x PF 15SIHP 25G 2x PF 30SIHP 25G 2x PF 50SIHP 25G v Documentation User Manual for PYR LASH 20 system Instruction Manual for Interchim Software Adjusting Protocol of Assembled Unit for Flash 06 DAD UV detector PDF document Operational Qualification and test report My documents Quick start for Interchim Software ONCE Installation Qualification x2 E 7 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE JuzlGe dl
19. ages causing faulty working of the detector 11 Carefully insert the new lamp assuring that it is correctly positioned Should you touch the glass of the lamp accidentally clean it thoroughly with a lint free cloth and alcohol 12 Screw it in securely and connect the plug in the 3 pin white socket 13 Reassemble the unit by making the same steps but in the reverse order After the lamp replacement recalibration and zeroing of the counter of operating hours must be carried out cf Service Info 39 6 6 Cleaning the detector flow cell A contaminated cell in lowered light transmission may increase noise level and make difficulties at zeroing The simplest cleaning method is flushing dismantled cell with suitable solvents The cell must be taken out from the instrument before washing 1 Follow the instructions 1 to 7 described in the previous chapter 6 5 Replacing detector deuterium lamp in order to properly disassemble the system 2 Disconnect the outlet tubing from the cell PEEK nuts 3 Remove easily the cell from the system by tilting the locking lever placing one hand under the cell to prevent it from falling to the bottom and tilting the locking lever with the other hand 4 Select the solvent type according to character of contamination by a series of mutually miscible solvents It is possible to use both organic and inorganic solvents and diluted solutions of acids 5 Flush the cell with pure solvent and
20. an appropriate waste bottle 3 3 Set up the column and the dry load on the column holder A large range of columns can be used with the system columns packed with normal or reverse phase with an external diameter up to 70mm as standard Using optional Extension kit for Large columns P N DU9671 this overall dimension can go up to 120mm The installation is simple 1 Unscrew lightly the knurled knob of the injection port holder in order to allow its vertical movement sA Move up the black POM holder on the vertical guidance rail and place the bottom fitting of the column on the Adapter ETFE or Stainless steel Luer lock female to 14 28 male located on the stainless steel bulkhead union 90 4 Move down the black POM holder and lock again the black ring of the fitting Adapter PEEK Luer lock male to 1 4 28 male to the top fitting of the column m Unlock the black ring of the fitting Adapter PEEK Luer lock male to a 28 male located under the injection port holder Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual To inject solid sample and make dry loading injection you have the possibility to use a pre column of which the maximum dimensions are 25cm in height and 70mm for external diameter Install it as follows 1 Unscrew lightly the knurled knob of t
21. baredy Bru ne hop Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e gl ie M 71 REN Module eese eese CS ele MC T P N and description of defective spare ln e Any further action ee lili EE RI NAMO enoras aaraa ES Signature gt Service report lt O Installation End of warranty visit O Warranty O Repair O Maintenance O Out of warranty ea er le ME NE E P Ville lei 1 ICS CO E gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty Systemi description M r o Sh ME Ville AC E NR EE EE EEEE eA E E E TE E E P N and description of defective spare e Any further action required Leia ce senos Una rax ret thus seras seeds cette ess s vectutuxssateeentdanebesancesvseanternectsaxtevendnersieenedebaredy Bru ne hop Signature gt Service report lt O Installation O End of warranty visit O Warranty O Repair O Maintenance O Out of warranty e ele e ME 71 M Module eese eese CS ele MC T P N and description of defective spare ln e Any further action ee lili EE RI NAMO enoras aaraa
22. c circuit rinsed and the inlets outlets corked The instrument must be carefully carried in its original box 6 Maintenance 6 1 Introduction This chapter describes actions that should be performed on a routine basis to ensure long term safe and trouble free operation of the system The frequency of the maintenance activities is dependent on the nature of the application the solvents used the quantity of the mobile phase delivered by the pump the level of cleanliness of the facility etc The pump has been designed for reliability and needs very little routine maintenance when operated correctly The following figure represents the liquid connections of the pump E SUCTION tubing 1 Check valve housing ae Delivery tubing Tee union e Sie Bukhead union e Pus Reduced accuracy of the pump may be due to seal wear or to a fault in the check valves This section provides information and procedures about how to replace consumable parts and how to maintain the instrument The removal of protective panels may only be performed by an authorized person 6 2 Removing a pump head When disassembling or reassembling the pump make sure that each component is clean and take care that the system is assembled in a clean environment Remove it if you need to replace its seal details hereafter 1 Purge the pump head with a suitable solvent or alcohol 1 propanol or 2 propanol 2 Switch off the module and disconnect it from
23. ck panel of the pump Serial port COM1 Switch 0 1 8 pin connector Axial fan mg E Fuses drawer Power plug Take the power cable for main voltage supply and connect it at the same time in the plugs of the unit control label PUMP and in the sockets of the pump module Link the unit control label COM1 PUMP and the pump module using the Sub D9 male female cable supplied Do not forget to set the connectors by fastening the two fixing screws Collector Back panel of the fraction collector External controls Switch 0 1 Serial port gt COM3 Analogic E Fuses drawer inputs for second detector acquisition Power plug Take the power cable for main voltage supply and connect it at the same time in the plugs of the unit control label COLL and in the sockets of the pump module Link the unit control label COM3 COLLECTOR and the collector module using the Sub D9 male female cable supplied Do not forget to set the connectors by fastening the two fixing screws Plug the AC power cable supplied in the general switch located to the left side of the system and then to the mains knowing that the connector depends on the destination country comply with country regulations in effect Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual For Am
24. ctrical supply 3 Remove the plastic cover to gain access to the pump heads 4 Disconnect the Suction and Delivery tubing and fittings 5 Unscrew the check valve housing using a spanner 17mm 6 Clean the check valves or provide yourself with news The cleaning allows to remove the particles present in the valves by placing them in an ultrasonic bath with methanol or acetone during approximately five minutes Ensure that the arrow is directed downwards during the cleaning Else damage to the surface quality or the ball and the seat will occur 7 Replace the check valves Whether it is for the inlet 10mm or the outlet 8mm make sure the arrow is always directed upwards Inlet Suction Outlet Delivery Check valve housing Check valve 8mm Check valve O 10mm mu BAIEBLEER 8 Hard tighten the valve housing in the head until hearing a little crunch which means that the new check valve is enough tight and the imperviousness properly done This noise corresponds to the collapsing of the top and bottom membranes 9 Connect the Suction and Delivery tubing and fittings D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC T l 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwiinterchim com ET LASH Lf 49 33 4 70 03 73 01 6 5 Replacing the detector deuterium lamp i 1 Purge the system with a suitable solvent with then alcohol and with air Deuterium lamp 2 Switch off t
25. erican system 110 120 VAC 50 60 Hz 6 2 A delay action fuses 2 x 6 3 A For European system 220 240 VAC 50 60 Hz 3 1 A delay action fuses 2 x 3 15 A ON OFF Switch Fuses Drawer Power Cable Check that all the ON OFF switches of secondary modules pump detector collector are on 1 and switch on the unit with the main ON OFF switch 3 7 Options and accessories Oo o AJ O Oo KR WD 10 14 12 13 14 ES 16 L 18 19 20 2 22 23 24 29 17 Video camera TLC plates holder P N GO2390 Fume enclosure P N PF4350 Quad system P N PF4360 Solvents level sensors 5 1 Global P N FSP720 or Solvents level sensors 5 5 Separated P N FSP730 Extension kit for large columns P N DU9671 Pre column holder P N GO9640 6 Ways manual valve W Loop Kit P N PF4330 Bar code reader P N GO2380 Di Enhancement tube device for 13 x 100mm Tubes P N DW3360 2ND Acquisition channel P N PF4320 Flash ELSD P N FI0320 Funnel rack set 1 unit P N FJ4331 or Funnel rack set 4 units P N FJ4330 Safety solvent caps kit 4 units P N DV2760 Safety waste cap with container 5L filter P N IO6930 or Safety waste cap with container 20L filter P N JO4500 Drainage system of the tray collector P N DZ7790 6 ways electric valve module P N FV2970 Semi Prep and Prep adaptation ki
26. eter dual wavelength DAD technology range 200 600nm v Collection Several types of racks available The complete system consists of following modules The 2 head pump As mentioned above the pump can deliver mobile phase at flow rates up to two hundred millilitres per minute with low residual pulsation It is a high performance double piston pump reciprocal movement Thanks to a system pulleys belt a stepper motor drives two special cams on which lean the two pistons Their motion generates a cycle and then creates the suction A static mixing chamber at the outlet ensures a suitable mixing of solvents whatever the working flow rate it is associated with a pressure transducer Mixing chamber Cleaning disc Leak sensor Electronic Pressure transducer d IU board CPU 2010 D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com ET LASH 33 4 70 03 73 01 Moreover an optic sensor can detect leakages in the pump drain pan The pumping is then interrupted and the user informed via the control software The 4 ways suction valve allows to perform quaternary elution gradients The injection unit and columns holder system This device gives the possibility to inject sample manually liquid or solid via dry load directly to the chromatography column installed Guidance rail Pre column or dry load Knu
27. he holder in order to allow its vertical movement 3 Move up the black POM holder on the vertical guidance rail and place the bottom fitting of the dry load on the Quick stop luer check valve of holder x Unlock the black ring of the fitting Adapter PEEK Luer lock male to a 28 male located above the injection port holder 4 Move down the black POM holder and lock again the black ring of the fitting Adapter PEEK Luer lock male to 1 4 28 male to the top fitting of the column 3 4 Prepare the fractions collection According to the type of collection required small or big volumes short or long fractionation etc the equipments to install are different The standard rack set 18 x 150mm P N PF4390 supplied is composed of four racks for 176 tubes in all it allows a total collection volume of 5 6 Litres with 180mm high tubes CAUTION Before any action take a diagonal plier and carefully cut the cable tie which holds the arm during the transfer Dispense arm Cable Tie Place first the tubes in the holes and then put the racks on the tray Thanks to notches machined on racks and positioning guides already mounted on the system their emplacement is easy and necessarily proper Positioning notch EMI Well placed rack Back guides Front guides D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interc
28. he system and disconnect it from the electrical supply It will be reconnected only when the panel will be installed back in its place 3 Let the lamp cools down fifteen minutes after switching it off 4 Remove tubing and connections from the detector module and remove it from the whole system you must first remove the pump module 5 Position the detector module on its side in order to view the bottom and unscrew the three M3 cylindrical head screws indicated below using an Allen wrench 2 5mm Take care not to lose M4 flat washers 6 Put the detector in the upright position and take off the front plastic cover 7 Then unscrew the two M4 domed head screws located on the back of the module using an Allen wrench 3mm and take off the top sheet cover 8 Loosen the screw on the socket of light tube behind the cell compartment using T 20 Torx adapter Turn the lamp socket to the horizontal position and tighten the same screw to fix it again 9 Take the lamp power supply cables and disconnect the 3 pin white connector Loosen and remove the two screws which hold the lamp using T 10 Torx adapter Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell re 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual 10 Remove the lamp from the lamp socket and provide yourself with a new one Never touch quartz bulb of the lamp with bare fingers to avoid dam
29. heck the tangential fan working to maintain a good air recycling inside the different modules Frequency Weekly or when inaccuracy flow rates solvents leaks or air bubbles are observed Yearly or when damaged parts are observed As often as possible specially after having pumped aqueous solvents or strong buffers Yearly Quarterly Yearly or when abnormal pressure flow rate fluctuation or noisy baseline are observed Quarterly or when pump head is disassembled Quarterly or when higher noise levels or decreased sensitivity are observed When its efficiency is unreliable Quarterly Daily Weekly or when inaccuracy flow rates solvents leaks or air bubbles are observed As often as possible specially after having pumped aqueous solvents or strong buffers Monthly Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com Manual interfine interchim fr 8 gt Warranty 8 1 Generalities Interchim guarantees the PUR LASH 20 for one year for parts and labour at the discretion of Interchim in normal conditions of use and installation from the date of signing of the installation report by the service provider approved by Interchim to install the machine and by the customer The PURIFLASH ZZ20 device is guaranteed against material and manufacturing defaults in normal conditions of use by
30. him com PUTI LesH 33 4 70 03 73 01 You can also equip your collector with an optional Enhancement tube device for 13 x 100m P N DW3360 It is designed for elevating the racks with small height tubes Its installation is simple too because you have just to emplace it on the collector tray using positioning guides Several models of rack sets are available find a detailed list on APPENDIX 1 It is important to maintain appropriate spacing of the dispensing needle above the tubes A space of 5 25mm between the end of the needle to the top of the tube is recommended To adjust the needle height turn off the system remove the racks and gently move the arm into an easy to access position Do not use the needle to relocate the arm as bending and mis alignment may occur Loosen the set screw and adjust the height of the needle and then tighten the set screw When the system is turned on the arm will return to its home position Collection tubing Dispense arm Dispense head Tightening screw 3 5 Position the stylus The stylus is supplied to control the system via computer touch screen A fixed holder allows to easily put it away when it is not used and its extensible cord linked to the holder gives the suitable length Stick the stylus holder just as you like in order to profit at best of its features i e to the left side of the system a
31. ll reserves to the transport firm before accepting the delivery of the device v The guarantee will not cover damage caused in accidents external events contingencies or force majeure due to negligence or a lack of surveillance by the customer or due to non compliance with safety rules v The guarantee will not cover damage if the maintenance procedures recommended by the manufacturer are not complied with v Interchim will not guarantee the results of the use of the PURI LASH 20 _ D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE NCAA T l 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com PUTI LASH 33 4 70 03 73 01 8 2 Limited warranty of consumable parts Item Part Number Warranty period Fittings unions amp tubing ETFE ferrule for 1 8 tubing PEEK 1 4 28 short nut for 1 8 tubing PEEK 1 4 28 long nut for 1 8 tubing Polytetrafluoroethylene Plug 1 4 28 for gradient valve ETFE Plug 1 4 28 Adapter PEEK Luer Lock Male to 1 4 28 Female Quick stop luer check valve Kel F mounting stud Adapter Luer Lock Male to 1 4 28 Male PEEK Adapter ETFE Luer Lock Female to 1 4 28 Male Adapter Stainless Steel Luer Lock Female to 1 4 28 Male Back pressure regulator 20psi 1 4bar PEEK tee union for 1 8 tubing Stainless steel bulkhead union 90 Suction tube ETFE 1 50m 1 8 ID 2 4mm PEEK nut ETFE ferrule spring 20cm x4 V1 Tube Gradient valve to Tee pum
32. p inlet ETFE 28cm 1 8 ID 2 4mm 2 PEEK nuts 2 ETFE Ferrules spring 20cm V1 Tube Pump outlet to column holder inlet ETFE 60cm 1 8 ID 1 6mm 2 PEEK nuts 2 ETFE Ferrules V1 Tube Column holder outlet to detector inlet ETFE 70cm 1 8 ID 1 6mm 2 PEEK nuts 2 ETFE Ferrules V1 Tube Detector outlet to collector inlet ETFE 30cm 1 8 ID 1 6mm 2 PEEK nuts 2 ETFE Ferrules V1 Waste outlet tube ETFE 1 50m 1 8 ID 1 6mm PEEK nut ETFE ferrule Tube Collection valve to collection needle ETFE 1m 1 8 ID 1 6mm 1 PEEK nut 1 TEFZEL Ferrule spring pipe gt Terms of use Normal tightening of the fittings Use of clean solvents and clean containers PFS680 PFS690 PFS700 PFS720 PFS730 PFS740 PFS750 PFS760 PFS770 PFS780 GV1680 FO3980 PFS710 PFS370 PFS810 102650 102660 102670 PFS860 PFS870 PFS880 Good positioning according to the instructions given in this manual gt Damages not covered Damaged thread due to unsuitable tightening Blocked passage due to uncleanliness of installation or unsuitable mobile phase Bended or cut tube due to unsuitable positioning Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual Item Part Number Warranty period Valves Quaternary
33. ps to zero Wear protection glasses Hazardous voltages are present inside the WARN N G instrument Disconnect from main power before removing screw attached panels SHOCK HAZARD Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr Removal of protective panels may only be performed by an authorized person When it is necessary to use a non original power cord plug make sure the replacement cord adheres to the color coding and polarity described in the manual and local building safety codes Replace blown fuses with fuses of the size and rating stipulated in the manual Replace faulty or frayed power cords immediately with the same type and rating Make sure that voltage sources and line voltage match the value for which the instrument is wired To ensure your own safety thus of your co workers and safe operation of the equipment observe the following instructions v Use a 3 wire mains socket with ground connection grounding is necessary to ensure operator safety and proper operation v Take care of inflammable solvents v Store the system after having passed a clean solvent in the hydraulic circuit when not in use Do not leave buffer in the system it may cause blockages and damage the seals on start up v Before moving the instrument the external connections have to be disconnected the hydrauli
34. rinse Locking extensively Flow cell lever 6 Reassemble the module by carrying out the same steps but in the reverse order After the operation check cell cleanness purging the system and verify there is no baseline drift D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE CTI T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com mo 7 gt Preventive measures 33 4 70 03 73 01 Upkeep schedule By considering the use of clean solvents and all precautions already mentioned in the chapter 6 Maintenance here is a table which regroup the recommended actions in order to assuring a good working of the system Operation Pump Check the suitable tightening of fittings strongly hand tight and the proper state of tubing not bended Replace the tubing and associated fittings Clean and rinse the gradient valve with suitable solution Replace seals and backup rings Clean the check valves Replace the check valves Clean the piston rods Detector Check the efficiency of the deuterium lamp Replace the deuterium lamp Clean the flow cell General Verify the cleanliness of all liquid containers Check the suitable tightening of fittings strongly hand tight and the proper state of tubing not bended Clean the injection valves and all parts in contact with solvents or samples with suitable solutions Rinse abundantly using a mixing of water and alcohol C
35. river Motor black Axial fan kit AC Power supply 230 24V 60W Support sheet Absorber Pump Collector fuses 2 5A 110V x 2 Pump Collector fuses 1 6A 220V x 2 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 4 70 03 73 01 Part Number PFS190 PFS090 PFS200 PFS120 PFS130 PFS170 PFS140 PFS150 101940 PFS210 101950 PFS320 PFS290 PFS220 PFS230 PFS990 PFS980 N 13 16 O C MO CO interfine interchim fr User Manual Item Part Number N Pump suite Mixing chamber PFS240 1 3 Seal for mixing chamber PFS250 4 Frit 20um for mixing chamber x 2 PFS260 6 Pressure transducer seal PFS270 5 Pressure transducer cable PFS280 2 Leakage sensor PFS300 Detector Flow cell 0 3 mm PFS960 PFS970 PFT050 GV3320 LV7070 Deuterium lamp Axial Fan Power Board Detector fuse 500mA 250V D D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC T l 33 470 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com Item Fraction collector Positioning guide for racks Fraction collector motor X axis cable Fraction collector motor Y axis cable X axis belt Y axis belt Pulleys 1 motor 1 receptor Collector opto sensor X axis Collector opto sensor Y axis ON OFF switch with EMI filter AC Power supply 230 24V 50W Pump Collector fuses 2 5A 110V
36. rled knob Liquid injection port Column The adjustable height holder of the black POM holder allows to adapt all types of columns A knurled knob can block this holder on the vertical guidance rail The bottom union is fixed An optional extension kit is available to use large scale columns 800g and 1 6Kg P N DU9671 Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr The UV diode array detector This instrument measures the amount of light absorbed by a liquid passing through the cell at a given wavelength It is equipped with a deuterium lamp The two wavelengths and the SCAN function available are selected within a large range from 200 to 600nm or from 190 to 840nm in option P N GO9630 Deuterium lamp Flow cell The fraction collector The fraction collector is placed downstream of the detector After the detection of the signal chromatogram the results obtained at the outlet of the column peaks can be directed to tubes disposed on racks A built in 3 way valve allows to choose between the collection or the rejection of the liquid Needle Electronic board collector The collector is provided with four stainless steel racks for 18mm diameter tubes as standard That represents in all 176 tubes and a volume of 5 6 Litres D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on
37. rsons The user should inform the distributor in case of problems and safety related matters that occur during the use of the instrument Alerts you to potential hazardous situations that could WARN N G result in serious injury or damage of the system This system is designed for chromatographic purposes It must be operated using appropriate solvents and within specified ranges for pressure flows and temperatures as described in this manual If it is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired When handling potentially hazardous solvents and samples it is important to follow standard laboratory safety procedures Common chemicals used in the laboratory can be toxic corrosive flammable or a combination of these properties A list of all safety aspects is outside the scope of this manual but the following points should be considered X Ensure adequate ventilation Wear eye protection Wear gloves when handling chemicals v Ensure all liquid containers are secure v No smocking or naked flames v Clean up all spillages W ARN N G m Eye damage could occur from particles or chemicals e Use proper eye protection EYE HAZARD If a line ruptures or a valve opens accidentally under pressure a potentially hazardous spray of liquid might be generated by the pump Never open a solvent line or valve under pressure Stop the pump first and let the pressure dro
38. s caused by external factors v The guarantee will not cover damage caused to the software or hardware due to contamination by an IT virus v The guarantee will not cover damage or failures caused by impact and or bad weather v The guarantee will be cancelled in case of damage caused by abnormal mechanical forces applied to the device and exceeding the limits defined in the user guide v The guarantee will be cancelled in case of corrosion to the device due to solvent leakage or samples v The guarantee will be cancelled in case of corrosion to electronic components caused by highly corrosive gas v The guarantee will not cover damage or failures caused by assembly dismantling modification or transport after initial installation v The guarantee will be cancelled in case of failure or damage due to non compliance with the closing and shutdown procedure for the device v The guarantee will be cancelled in case of failure or damage due to wrong installation v The guarantee will be cancelled in case of failure or damage due to wrong AC power supply v The guarantee will be cancelled in case of failure or damage due to mechanical force to the unit v The guarantee will be cancelled in case of failure or damage due to acts of nature v The guarantee will be cancelled in case of failure or damage due to non compliance with safety procedures v The customer is liable for transport risks In case of damage during transport the beneficiary must issue a
39. s showed on this picture Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr 3 6 Make the connections electrical and data transmission The complete system is supplied with appropriate fuses and is ready to operate at the line voltage of the shipping destination The Computer module serves as the primary point for power distribution and data collection The ports on the modules are labelled to ensure proper connections Using the cables provided connect each module to the unit control Unit control Back panel of the unit control communication ports Power sockets Detector Back panel of the detector 24 V round connector E 534 Axialfans Serial port gt COM2 Take first the special cable for 24V supply and screw the round male connector in the suitable socket label DET of the unit control Screw the other end round female connector in the back panel of the detector Link the unit control label COM2 DETECTOR and the detector module using the Sub D9 female female cable supplied Do not forget to set the connectors by fastening the two fixing screws D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Dui ella ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com La ZS LASH d 33 4 70 03 73 01 Pump Ba
40. st of spare parts Blow up views Item Part Number N Fittings unions amp tubing ETFE ferrule for 1 8 tubing PEEK 1 4 28 short nut for 1 8 tubing PEEK 1 4 28 long nut for 1 8 tubing Polyt trafluoro thyl ne Plug 1 4 28 for gradient valve ETFE Plug 1 4 28 Adapter PEEK Luer Lock Male to 1 4 28 Female Quick stop luer check valve PFS680 PFS690 PFS700 PFS720 PFS730 PFS740 PFS750 PFS760 PFS770 PFS780 GV1680 FO3980 PFS710 PFS370 Kel F mounting stud Adapter Luer Lock Male to 1 4 28 Male PEEK Adapter ETFE Luer Lock Female to 1 4 28 Male Adapter Stainless Steel Luer Lock Female to 1 4 28 Male Back pressure regulator 20 psi 1 4 bar PEEK tee union for 1 8 tubing Stainless steel bulkhead union 90 Suction tube ETFE 1 50m 1 8 ID 2 4mm PEEK nut ETFE ferrule spring 20cm V1 Tube Gradient valve to Tee pump inlet ETFE 28cm 1 8 ID 2 4mm 2 PEEK nuts 2 ETFE Ferrules spring 20cm V1 Tube Pump outlet to column holder inlet ETFE 60cm 1 8 ID 1 6mm 2 PEEK nuts 2 ETFE Ferrules V1 Tube Column holder outlet to detector inlet ETFE 70cm 1 8 ID 1 6mm 2 PEEK nuts 2 ETFE Ferrules V1 Tube Detector outlet to collector inlet ETFE 30cm 1 8 ID 1 6mm 2 PEEK nuts 2 ETFE Ferrules V1 Waste outlet tube ETFE 1 50m 1 8 ID 1 6mm PEEK nut ETFE ferrule Tube Collection valve to collection needle E
41. t P N GO2400 2 columns selection switching valve P N LO6640 Backflush valve P N LO6650 2 columns selection switching valve Backflush valve P N LO6630 6 ways electric w loop 2mL loop by default Backflush valve module P N LO6730 6 ways electric w loop 2mL loop by default 2 columns selection switching valve module P N LO6740 6 ways electric w loop 2mL loop by default 2 columns selection switching valve module Backflush valve module P N LO6750 Air purge P N FV2980 RI IOTA 2 P N FSQ600 D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC T l 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com 26 Mass Spectrometer interface P N JO1090 27 CarouXel P N JO3750 28 Autosampler P N LO8850 29 Ballasting kit P N DZ7360 30 Racks set 31 2 fraction collector coupled P N JV0950 Designation Items 1 Solvent level sensors 5 1 global Communication interface 5 1 cable Sensors cables x 5 Straps 80cm x 5 Removable cable ties x 5 Power adapter Hardware Installation procedure 1 Solvent level sensors 5 5 separated Communication interface 5 5 cable Sensors cables x 5 Straps 80cm x 5 Removable cable ties x 5 Power adapter Hardware Installation procedure 2 Extension kit for Large columns Long black POM holder grub screw
42. tylus FK2400 AC power supply 230 24V 150W PFS550 UC axial fan V2 LV9380 Multi stage performance EMI filter PFS570 General ON OFF switch PFS590 General fuses x 2 3 15 A 220V PFTO10 General fuses x 2 6 3 A 110V PFT100 Serial communication cable male female PFS600 Serial communication cable female female PFS610 Distribution power cable male female for main voltage PFS620 Distribution power cable male female for 24V PFS630 Power cord FR PFS640 Power cord UK PFS650 Power cord US PFS660 10 gt Specifications v Model name PYRI LASH 4230 system v Serial number XXXX X XX v Flow rate range 1 to 200mL min v Flow rate performance 1 accuracy with H O degassed at 20 C Repeatability better than 0 5 v Gradient former Linear response from 2 to 9896 v Injection Liquid manual loop or Solid v System protection Software Pressures Min Max adjustable Leakage detection v Maximum pressure 30 bar 435 psi Control via PC software INTERCHIM SOFTWARE VX X v Storage up to 32 methods in software memory plus 160 Go of hard disk memory v AC mains supply voltage 220 240VAC 50 60Hz or 110 120VAC 50 60Hz v Electrical protection Delayed action fuse 3 15A or 6 3A v Power distribution TT or TN power system only transient over voltages according to installation category Il Class 1 equipment requires mains socket plug with protective earth terminal v IP Code IP20 v Dimensions 540 x 700 x 480mm Width
43. uip yourself with the loop and connect it between the ways 1 and 4 of the valve The loading and injection can be done at any time during a method when the pump runs or not Nevertheless it is advised to not combine this procedure with the use of a pre column for solid injection Here is the procedure 1 Put the 6 way valve to the LOAD position aD Fill a syringe with the volume of sample wanted and place it in the fitting Adapter Luer lock female to 74 28 male way 1 2 Fil the loop with the content of the syringe If the quantity injected is greater than the capacity of the loop the surplus flows in the waste tube way 3 DD owitch rapidly the valve to the position INJECT The loop gets in the hydraulic circuit of the pump the sample is pushed in the chromatography column by the solvents pumped For instance at that time click manually on the button START to start the method D a 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE TAC T l 33 470 03 88 55 Fax 33 4 70 03 82 60 www interchim com PO LASH PA 219 33 4 70 03 73 0 5 gt Safety regulations This equipment is built according to the E U security standards However risks and dangers could remain if the system is used in a different way from which it is intended or if it is used by untrained personnel The PURIFLASH ZZ 20 should only be used by laboratory trained or experienced pe
44. ul rei 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURFLASH d 33 4 70 03 73 01 3 Installation 3 1 Assembling the system Select a suitable location by ensuring adequate air space 15cm around the system to provide ventilation of the controller the pump the detector cooling fans Its total weight is approximately 60kg Air extractions v Assemble the system in the following order Fraction collector 25kg Unit control 15kg Detector 7kg Pump 8kg Column Holder 3kg Solvent tray 1kg v Put then the stainless steel solvent tray supplied on top to dispose solvents up to five They cover the totality of the system with a drainage system and their design avoids any leakage risk Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com interfine interchim fr User Manual v Two possibilities to fix the column holder as below Insert the column holder at the bottom of the collector Put the binding column and the screw on the middle of the column holder A Configuration 1 Configuration 2 3 2 Install the tubing and prepare the containers The tubes situated outside the housing are delivered apart in order to avoid all deterioration during transfer they are already equipped with the suitable fittings nut ferrule to connect them easily to the system Regarding the main circuit
45. ump I 27cm 1 8 ID 2 4mm as shown below pump inlet Pump outlet Gradient valve outlet Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com User Manual interfine interchim fr Pump to column holder Using the 60cm x 1 6mm ID tube insert one end into the pump outlet and the other end into the column holder inlet Position the tubing as shown below then seat the ferrules and hand tight each fitting Ai Pump outlet Column holder inlet Column holder to detector Using the 70cm x 1 6mm ID tube insert one end into the column holder outlet of the stainless steel bulkhead union 90 and the other end into the detector inlet Position the tubing as shown below then seat the ferrules and hand tight each fitting Detector inlet Column holder outlet D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com PURUFLGSH 33 4 70 03 73 01 Detector to fraction collector Using the 30cm x 1 6mm ID tube insert one end into the detector outlet and the other end into the fraction collector inlet Seat the ferrules and hand tighten Detector outlet Fraction collector inlet Waste Using the 1 5m x 1 6mm tube attach the end with the fitting into the fraction collector waste port and hand tighten On the other end insert into
46. ww interchim com Wait for Inj The liquid injection is done directly through the chromatography column by using a syringe In the panel Settings of the method edited select Equilibration in the section Injection Mode According to the type of column chosen a time and a flow rate are written by default they depend on its parameters but you can modify them if needed Unlock the black ring of the fitting Adapter PEEK Luer lock male to 28 Male from the Quick stop luer check valve 3 Remove the syringe After having launched the method RUN the equilibration phase starts That allows to condition the column with the solvents used At the end of the time or at a click on START the pump stops and the following dialog box appears on the Screen Place the syringe filled with sample on the Quick stop luer check valve and inject manually the desired quantity ve Screw the fitting Adapter PEEK Luer lock male to Ye 28 Male Answer Yes to the question of the software The method Stans User Manual PUES LASH Zt 33 4 70 03 73 01 4 3 Make a solid injection The solid injection is done via a dry load in which the solid sample has been previously deposited The dry load is emplaced directly on the Quick stop luer check valve An optional separated holder is available to use other columns and have a support comparable to the one of the main column P N PF4
47. x 2 Pump Collector fuses 1 6A 220V x 2 Black POM for column holder fixed x 2 screws Kai 211 bis avenue JF Kennedy BP 1140 03100 Montlucon FRANCE Duell ra 33 4 70 03 88 55 Fax 33 4 70 03 82 60 wwwinterchim com 33 4 70 03 73 01 Part Number PFS400 PFS410 PFS420 PFS430 PFS440 PFS450 PFS460 PFS470 PFS330 PFS480 PFS990 PFS980 102960 N interfine interchim fr Item Column holder ETFE ferrule for 1 8 tubing PEEK 1 4 28 long nut for 1 8 tubing Adapter PEEK Luer Lock Male to 1 4 28 Female Quick stop luer check valve Kel F mounting stud Adapter Luer Lock Male to 1 4 28 Male PEEK Guidance part for flash configuration otainless steel union of black POM holder Black POM holder grub screw Stainless Steel bulkhead union 90 Adapter ETFE Luer Lock Female to 1 4 28 Male Or Adapter Stainless Steel Luer Lock Female to 1 4 28 Male PEEK Ring Outlet column holder Binding column D 211 bis avenue JF Kennedy BP 1140 03100 Montlu on FRANCE Dui ella T l 33 4 70 03 88 55 Fax 33 4 70 03 82 60 www interchim com Part Number PFS680 PFS700 PFS740 PFS750 PFS760 PFS770 FO4400 FV6560 101290 PFS370 PFS780 GV1680 102690 02700 N 3 5 Oo MM User Manual PUGUSLASH 33 4 70 03 73 01 Item Part Number N General Central Processing Unit Touch screen PFS520 USB hub PFS530 USB extension cord I 0 5cm PFS540 S
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