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H201-NIKON-NZ100-200-500-N

1. www oko lab com Page D H201 NIKON NZ100 200 500 N User Manual March 2015 2 Lab Tek II 1in x2in chambered cover glass 2 60mm Petri dish 4 35mm Petri dish 1 1x3in chamber slide and 2 35mm Petri dish 1 Lab Tek 1in x2in chambered cover glass and 2 35mm Petri dish 1 Lab Tek II 1in x2in chambered cover glass and 2 35mm Petri dish 1 Lab Tek 1in x2in chambered cover glass and 1 60mm Petri dish 1 Lab Tek II 1in x2in chambered cover glass and 1 60mm Petri dish 1 1x3in chamber slide and 1 60mm Petri dish 1 60mm Petri dish and 1 35mm Petri dish Magnetic lock to hold the standard MW plates in the chamber Magnetic lock to hold the low profile MW plates in the chamber i gt gt GS35 M 4x35 M 2x60 M GS60 M 1x60 M LABTEK 60 M For 1 1 x3 chamber slide and For 4 35 mm For 2 60 mm For 1 60mm petri dish and For 1 60 mm For 1 Lab Tek 1 x2 chambered 2 35mm petri dishes petri dishes petri dishes 1 1 x3 chamber slide petri dish cover glass and 1 60mm petri dish 1x35 M 6035 M 1xGS M 2xGS M 1xGS 2xLABTEK M 1xLABTEK M For 1 35 mm For 2 35 mm petri dishes and For 1 1 x3 For 2 1 x3 For 1 1 x3 chamber slide For 1 Lab Tek petri dish 1 60mm petri dish chamber slide chamber slides and 2 Lab Tek 1 x2 1 x2 chambered chambered cover glass gt glass LABTEK 35 M LABTEK 60 M 1xLABTEK M 2xLABTEK M LABTEK II 35 M 2xLABTEK II M For 1 Lab Tek 1 x2 For 1 L
2. the Sliding Lid with and without chamber riser Figure 4 illustrates how to remove Sliding Lid when a different Lid is necessary NOTE Sliding Lid MUST BE REMOVED when using any other Lid The Sliding Lid is fixed onto the chamber with 4 screws Screws location is indicated by letter A in Figure 4 image 2 and 3 Keep Sliding Lid fully closed to access screws labeled A in image 2 Open Sliding Lid to access screws labeled A in image 3 Sliding Lid Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane and upper side of the glass lid without the chamber riser Chamber top view f Figure 4 Assembly of the sliding lid www oko lab com Be H201 NIKON NZ100 200 500 N User Manual March 2015 3 2 Koehler Lid The Koehler Lid is a glass lid reducing the chamber s height to 21 mm and allowing imaging under Koehler illumination The Koehler Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 5 shows chamber dimensions with Koehler lid with and without chamber riser H201 KOHLER LID Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 2 and upper side of the glass lid without the chamber riser Chamber top view Figure 5 H201 NIKON NZ100 200 500 N H201 KOEHLER LID 3 3 Injection Lid The Injection Lid is a glass lid with two sma
3. CK 22 5 and MW LOCK 16 5 Duo MW 22 5 LOCK To be ordered separately Typically used for 6 12 24 48 MW 16 50 e MW 16 5 LOCK To be ordered separately Typically used for 96 MW Figure 16 Magnetic locks for MW plates To be ordered separately Chamber Riser increases chamber s height and is REQUIRED with 6 12 24 48 MW plates regardless of the Lid em ployed Figure 17 shows how to mount and screw the chamber riser Screws location is indicated by letter A Chamber Riser Figure 17 Riser Assembly www oko lab com el 2 H201 NIKON NZ100 200 500 N User Manual March 2015 9 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H201 NIKON NZ100 200 500 N Silicon tubing connects to a gas input brass opening located on a corner of the H201 NIKON NZ100 200 500 N See Figure 18 Connect by gently pushing silicon tubing onto brass opening Gas Input Figure 18 Connection with gas supply 10 Working with Perfusion The Chamber Riser included with H201 NIKON NZ100 200 500 N features 12 perfusion holes for the insertion of perfu sion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as necessary before introducing perfusion tubing NOTE Working with Perfusion is possible with Mad City Labs NZ100 200 ONLY Figure 19 shows loc
4. H201 NIKON NZ100 200 500 N User Manual March 2015 H201 NIKON NZ100 200 500 N Compatible with the following XY stages Compatible with the following Okolab Controllers Mad City Labs NZ100 200 500 N on Nikon me H201 T UNIT BL chanical or motorized stages TI S E and TI S ER www oko lab com Page 1 H201 NIKON NZ100 200 500 N User Manual March 2015 Index Me Components and CNS SIONS ce ee ae ee ae sera 3 2 Sample Holders u 0 aa ee nee aa area een 3 2 1 Available Sample Holders suessue see srense covers as ob enouucevecerccevacevacavasauncetieerncorowececorunes caerenecarorenores 3 3s ANallable Eds ausser Ar Ar RR Raser 4 Li SANS N u a rer 5 3 2 Koehler CIT S 6 3 3 IDJECLION di nee ee TE E E E re 6 3 2 LASER IBler lock Eid anne 7 4 Insertion of the Sample Feedback Temperature SeENsor ccccccccccccccccccccccccccccecccecccecccecccecceecceece 8 5 Insertion of Sample Holder into Chamber un en 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic LOCKS ccccceecccccccccscccesccceeccs 9 7 Working with 1x3 and 1x2 chamber slides Magnetic lOCKS cc cce ccc ceecccceccccncccceccceecccescccesces 11 8 Working with MW Plates Magnetic locks and Chamber riser cccccccccccccccccccccecccecccecccecccecccecees 12 9 Connection of the Gas SUB DNY anne 13 10 Working with Perfusion ee ee ee ee secs ceneuesacestuesacossoocasoes 13 11 Connection of
5. ab Tek 1 x2 For 1 Lab Tek 1 x2 For 2 Lab Tek For 1 Lab Tek Il For 2 Lab Tek Chambered cover glass Chambered cover glass Chambered cover glass 1 x2 chambered 1 x2 chambered cover glass 1 x2 chambered cover glass and 2 35mm petri dishes and 1 60mm petri dish cover glass and 2 35mm petri dishes Figure 2 Available sample holders The following heated glass Lids are available for H201 NIKON NZ100 200 500 N H201 SLIDING LID For easy sample loading and pipetting Included in code H201 NIKON NZ100 200 500 N Compatible with Mad City Labs NZ 100 200 ONLY H201 KOEHLER LID Reduces chamber height to 21 mm and allows imaging under Koehler illumination H201 INJECTION LID Glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample Compatible only with sample holder 1x35 H201 LASER INTERLOCK LID Glass lid with safety switch Connects to laser controller and automatically turns laser off when lid is lifted Compatible only with sample holder 1x35 OPTIONAL not included with H201 NIKON NZ100 200 500 N www oko lab com Page H201 NIKON NZ100 200 500 N User Manual March 2015 3 1 Sliding lid The Sliding Lid is a glass lid allowing for easy sample loading and pipetting The Sliding Lid is screwed onto the cham ber NOTE H201 SLIDING LID is compatible with Mad City Labs NZ100 200 ONLY Figure 3 shows chamber dimensions with
6. and 60 mm dish Figure 12 and Figure 13 respectively Figure 11 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com sie H201 NIKON NZ100 200 500 N BD Falcon 35x10 Willco 35x10 Figure 12 Ring selection scheme fo User Manual March 2015 Grainer Petri35x10 Ibidi p Dish 35 mm low OO OQO Corning 35x10 mm MatTek P35G X14 X r 35 mm dish C Corning 60x15 mm Ibidi Dish 50 mm low MatTek P50G X30 X QO QO BD Falcon 60x10 Grainer Petri 60x15 Willco 60x15 Figure 13 Ring selection scheme for 60 mm dish www oko lab com page LO H201 NIKON NZ100 200 500 N User Manual March 2015 7 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 bape 1 www oko lab com H201 NIKON NZ100 200 500 N User Manual March 2015 8 Working with MW Plates Magnetic locks and Chamber ris er Magnetic locks hold MW plates in place into chamber illustrated in Figure 16 NOTE Magnetic locks must be ordered separately NOT INCLUDED Product codes MW LO
7. ation of perfusion holes Perfusion dedicated opening 1 Frontal view 2 3D view Figure 19 Perfusion www oko lab com bape 3 H201 NIKON NZ100 200 500 N User Manual March 2015 11 Connection of the Chamber with Z Piezo stage Follow the steps shown in the images of Figure 20 and listed below in order to correctly connect the chamber with Z piezo stage 1 Place the chamber on the stage and tighten 2 captive screws See Image 2 of Figure 20 Captive screws housings are indicated with letter B in Image 2 of Figure 20 2 Use a1 5mm metric Allen Wrench to tighten the two captive screws B in Image 3 while keeping the chamber sliding lid closed See Figure 20 Image 3 3 Tighten the other captive screws B in Image 3 while keeping the chamber sliding lid open Captive Screws Figure 20 Connection of the Chamber with XY Stage www oko lab com page LA
8. ll openings sealed with flexible plastic allowing injection or permanent access to the sample NOTE Compatible with sample holder 1x35 ONLY The Injection Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 6 shows chamber dimensions with the Injection Lid with and without chamber riser www oko lab com s H201 NIKON NZ100 200 500 N User Manual March 2015 28 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 22 and upper side of the glass lid without the chamber riser Chamber top view Figure 6 H201 NIKON NZ100 200 500 N H201 INJECTION LID 3 4 Laser Interlock Lid The Laser Interlock Lid is a glass lid with a safety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid with and without chamber riser Distance between focal plane 29 and upper side of the glass lid with the chamber riser Distance between focal plane 23 and upper side of the glass lid without the chamber riser 0 H201 LASER INTERLOCK LID Chamber top view Figure 7 H201 NIKON NZ100 200 500 N H201 LASER INTERLOCK LID www oko lab com Be H201 NIKON NZ100 200 500 N User Manual March 2015 4 In
9. sertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201 NIKON NZ100 200 500 N see Figure 8 Frontal and 3D views Temperature Sensor dedicated opening Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 8 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 9 Figure 9 Introduction of the Specimen Holder inside the Chamber Base www oko lab com pase H201 NIKON NZ100 200 500 N User Manual March 2015 6 Working with 35 or 60 mm Petri Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder lish Spacing Rings and Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Figure 10 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 11 shows how to position the ring Ring selection guide diagrams rings for 35
10. the Chamber with Z Piezo stage sesescecesoecesoecesoecesoecesoecesoecesoecesoeceseeceseeceseeceo 14 www oko lab com es H201 NIKON NZ100 200 500 N User Manual March 2015 1 Components and dimensions H201 NIKON NZ100 200 500 N includes the following components Chamber main body Sliding glass lid for easy pipetting Chamber riser It is a removable frame increasing the height of the chamber from 24 to 30 mm often re quired when using multi well MW plates Sliding Lid 3 Distance between focal plane and upper side of the glass lid with the chamber riser 24 Distance between focal plane and upper side of the glass gt y lid without the chamber riser K 3 Sample Holder Order Separately Chamber Riser aut Chamber top view Chamber Base Figure 1 H201 NIKON NZ100 200 500 N Components and Dimensions 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available 109 70 NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish 1 Nunc and Greiner T25 flask 2 35mm Petri dish 2 1x3in chamber slides 2 Lab Tek 1in x2in chambered cover glass

H201-NIKON-NZ100-200-500-N

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