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MPW-380/R - MPW MED. INSTRUMENTS Spółdzielnia Pracy

1. Selection of rotor and bucket Selection of rotor from list browsing of rotors and their parameters Assigning of PARA work parameters and CONFIG configuration of centrifuge Assigning settings takes place in two stages It is necessary in the first place to choose the zone in which the parameters will be set Available zones SPEED RCF TIME TEMP PROG PROG LIST ROTOR NUMBER BUCKET NUMBER ROTORS LIST PARA CONFIG The zone is selected by pressing the SET key first from chosen zones will begin to blink it is SPEED or RCF depending on the mode of work of the screen and choosing the suitable zone with UP or DOWN keys After choosing the zone pressing the SET key again will cause proceeding to parameters setup The settings may be changed with keys UP and DOWN accordingly Pressing the SET key ends the setting and causes the chosen value to be entered to the driver s register 19 Exemplary change of TIME settings a press the SET key First zone SPEED will begin to blink b press the UP key two times selection of TIME zone Blinking TIME zone mz c press the SET key starting the change setup in the TIME zone The SET inscription is highlighted Blinking altarpiece mode of programming setups in the undergoing a change chosen zone 20 d increase or decrease the setup value with UP and DOWN keys Pressing in and supporting the key longer than the second makes switching on repetition of Key If the Key is
2. press SET key choose DELETE option Call service e Jt is not possibile to open save program and change any parametr 55 Emergency cover release In the case of e g mains failure it is possible to open cover manually At first one must be sure that rotor is not in the move use inspection glass On the left side of the casing there is a hole with a plug that shall be unplugged Insert T handle Allen key 17779 into the hole and turn it counterclockwise ZA The cover can be unlock and opened only when the rotor is in the rest state 9 2 Work safety inspection For safety reasons inspections of the centrifuge carried out by the authorized service at least once a year after the period of warranty The reason for more frequent inspections could be corrosion inducing environment Examinations should end with issuing Report of validation the check on the technical state of the laboratory centrifuge It is being recommended to establish Technical passport or Log of the apparatus in whom every repairs and reviews are being registered Both these documents should be stored in the place of use of the centrifuge 9 3 Inspection procedures carried out by the operator Operator has to pay special attention to the fact that the centrifuge parts of key importance due to safety reasons are not damaged This remark is specifically important as for 1 Motor suspension 2 Motor axis concentricity 3 Fixing the pins in the bu
3. centrifuge Cat No 10380 10380R AM f W 20380 R ENG user manual Cat No MED INSTRUMENTS 2012 06 12 USER MANUAL ol ol 0401 1 o gq PALM peor LABORATORY CENTRIFUGE MPW 380 R A Read this before use MPW Med instruments 46 Boremlowska Street 04 347 Warsaw Poland tel 48 22 610 81 07 service fax 48 22 610 55 36 IVD mpw mpw pl www mpw pl Warning sings and hazard icons WARNING Warning of potential injury or health risk DANGER Biohazard with potential for risk to health or death as a consequence DANGER Risk of explosion with potential for severe injury or death as a consequence A DANGER Risk of electric shock with potential for severe injury or death as a consequence Statement of Conformity The following machine is in accordance with the regulations of the EU Directive 98 79 EC and with the harmonized standards PN EN 61010 1 and PN EN 61010 2 020 Contents APPLICATION oori A A GEO AOR OWAD OOO 5 POC ANICACEDZOE z A A A ROI a er 5 Z1S ACCESSORIES ia EE A AG CA 6 2 1 1 Basic accessories delivered with every centrifuge eeeee aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa wawa a iiaainiei 6 2A 2 Opna LAC CESSO IES Saba OOO AOLO REAR is WEAK 6 2 25 EXPLOITATION MATERIAL Sinuind iR AB Zad lada 9 ANS TAE LEA TION caiman O E eea nicas 10 SU WINPA CKING OF THE CENTRIFUGE an 10 Der GG U CO JRE 10 3 3 CONNEC TION TO MAIN Sa 10 SA CU
4. l gt 03 11 12 13 Connect the centrifuge to the mains master switch on left side of the centrifuge Open the cover of the centrifuge by pressing the COVER key Prior to putting the rotor in one has to check 1f the rotating chamber is free of impurities e g such as dust glass splinters residues of fluids that must be taken away One shall fit the rotor on the motor shaft driving 1t home on the cone Caution Fitting the rotor too shallow will result in lack of identification of the rotor after start of the centrifuge displaying the error message see p 7 7 1 and stopping the centrifuge Screw in the bolt for fixing the rotor clockwise and screw it tightly home with the supplied spanner for the rotor Swing out rotors have to be provided with the buckets in all seats One should remember that every buckets swings individually Bucket suspension studs should be lubricated periodically with technical petroleum jelly In the case of rotors designed with the cover they must not be used without it Rotor covers must be closed exactly Rotor covers ensure smaller drags of the rotors proper setting of the test tubes and airtight sealing One should use only buckets intended for selected types of the rotor see p 2 1 Accessories Fill test tubes outside the centrifuge Put on or screw the caps on vessels and rotors if applicable In the case of centrifuging in an angle rotor test tubes buckets have to be filled
5. without cooling Its construction ensures easy operation safe work and wide range of applications at laboratories engaged in routine medical analyses biochemical research works etc This centrifuge is not biotight and therefore during centrifugation of preparations requiring biotightness one has to use closed and sealed containers and rotors In the centrifuge it is prohibited to centrifuge caustic inflammable and explosive preparations 2 Technical data MPW Med instruments 46 Boremlowska Street 04 347 Warsaw Poland MPW 380 MPW 380R 12 A 16 A cooling medium _ __ RO 100 18000 10s resolution 31150 x g 5 s 9 min 59 s 1s resolution 10 min 9 godz 59 min 1min resolution running time counting till start effective centrifuging time calculation short time operation mode yes continuous operation mode yes operation programs no yes 2500 RPM 4 C yes adjustable curves acceleration 10 curves 4 points deceleration 10 curves 4 points yes ambient conditions PN EN 61010 1 1 4 1 set up site indoors only ambient temperature 5 40 C humidity maximum relative humidity lt 80 excess voltage category PN EN 61010 1 pollution degree PN EN 61010 1 safety area 300 mm width 515mm 715mm depth 650 mm 650 mm height 455 mm 455 mm CFC HCFC free program no 1 kkk option 2 1 Accessories 2 1 1 Basic accessories delivered with every centrifuge Cat No Accessori
6. 7 6 12 10 Cycles preview of spinning parameters of 10 last cycles eeeeeeea aaa aaa aaa aaa aaa aaa 44 7 6 13 Contact Us fold with data of the MPW company eeeeeeee aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa aaa nana nnnnnccnnnnnnnnnnnns 45 7310 AR TING OF CEN TRIEU GING pdas 45 AZ ATTY OFS SCT E RA O A O 46 7 7 2 Stabilization of the temperature of the centrifuge oooooonnnnnnnnncncnnnnnnnnnnnnnnnnnnnnnnnnnncnnnonnnnnnn ono nnnnnnnnnrnnnccnnnnnnnnnnnss 48 7 7 3 Screens informing about the end of CentrifUging oooonnnnnnnnnnnnnnnnnnnonnnonnnnnnnnnnnnnnoncccnnnnnnnnnn nro nora nana nnnnnccnnnnnnnnnnnss 48 ELA AAA ON GUL TIVES SCO wROCK OOO T SG aw ge 49 2 0 CHANGE OF SETTINGS IN ZONES pausieren rae a l kW ER GOO 49 RZUNBALANE NK AN Z O A Z NEA 50 7 10 EMERGENCY STOP dl adi 50 7 11 COMPLETION OF THE CENTRIFUGING 0 AAA CAC O EA 50 7 12 MATHEMATICAL RELATION Sailor 50 TALA REE FClQHv eG CONT DAA ic 50 7 12 2 Nomograph of relationship rotational speed centrifuging radius RCF Drawing No 5 ccccccc cece eeeeeee 50 PADIERNA O WOK EA R EE BT EEE 50 7 13 TEMPERATURE IN ROTATIONAL CHAMBER 40 dE wio akc a 51 CLEANING DISINFECTION MAINTENANCE wici ii Ewa ii 52 9 1 CLEANING OF THB CENTRIFUGE zai ko o ka ai 52 5 2 CEEANI GOFTHEACCESSORIES iniii a AT i OT EA CLA 52 3 33 LUBRICA ON dd RGG A 32 5 4 GLASS TUBE CRACKING a AA A A 52 8 5 STERILIZATION AND DISINFEC
7. A RA A E WORA 19 1 0 SETTING UP PARAMETERS Aaaa Goa akad dc 19 Ta DESCRIP LION OF ZONE Ss OO A AK OO OE ARA 21 VEN SC CO ssl ces ast id toca anes i ton A ZOO is ii ci 21 TADO AN AAA a 21 DAS TUMOR AS E A E AI E A A NS AN E E NAE A AAE ATTA 22 TAA CN A TELMO ls ll a ol 23 TAD POS USCIS DEO FOME A Ot OJ PRE wad E E E E ARJ E T 2 TAO PASC OF ME USED O dos 24 7 4 7 Program of preliminary refrigerating ccccccccccccccccccccce aaa aaa aaa aaa aaa aaa aaa nn nn non anna nnnrnnncnnnnnnnnnnnns 25 7 4 8 Changing acceleration deceleration characteristics numbers ACC DEC 10 19 ccccccccccccesccccccccecceceeeeeeaaaes 26 TAD ROOT RUCK CU Od A IN OOOO AEON OO ashe 26 TA LO ASCO TOTO AA ARS O WR OO OO AA ONAR OE 26 7 5 PARA ZONE OF SETUP OF OPERATIONS PARAMETER 0cccccsscccccsecccaeccecuecceeeeeceeaeeccesueecceseeecesueeecesaeeceeaueecesaes 2 7 5 1 Description of the function in the Para fold ooonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnoncnnn nono nono nn nono nan nnnnrnccncnnnnnnnnnnos 28 7 5 2 Acceleration and Deceleration characteristics ooonncnninnncnnnnncononacinnonaronnnnorononarocnnnnrcnnonoronnnarrnnnnarcnnonarcnnnaricnnns 28 BRI HR 2111 AAA A A Z 29 7 3 4 Densi SAMPLE ANS A A dia 29 7 5 5 Cooling refrigerating of the centrifuge Chamber oonnnnnnnnnnicinnnnnnnnnnnnnnnnnnnnnnnnnnnnccnnnnnnnn nn non nono nnnnnnnnncncnnnnnnnnnns 30 7 5 6 Open lid after run Automat
8. Centrifuge was displaced during operation Switch ON the centrifuge again after opening and closing the cover Check if rotor number in started program is consistent e After stopping error rotor message is displayed with the number of the rotor installed in the centrifuge rotor status if there are coding magnets inserted e Centrifuge does not recognize the rotor and does not Switch the centrifuge OFF then ON and check stop correctness of loaded program 54 4 It is not possible to open the cover e dot on the display is blinking after pressing Rotor is still rotating Wait for stopping of the COVER key single tone is audible rotor and displaying of the square symbol e Nothing is displayed Check the centrifuge power supply e dot on the display is not blinking but cover can not Call service be opened gt Centrifuge is working and mains failure e The message will be displayed on the display about Wait for stopping of the rotor clear the error by the decay of tension pressing the SET key MPW 380R e The message will be displayed on the display about Check whether the sensor is connected to the the error of temperature sensor steering plate correctly e The sensor is connected correctly and the error is still applying 7 Error of the exceeding the temperature in the Remedy chamber MPW 380 R 8 It is not possibile to change any paramater ak enter the list of user programs hi select no 1 program
9. SHORT key serves for short duration work the centrifuge is working as long as the key is pressed 4 START diode is on of the moment of pressing the START key until pressing the STOP key 5 START key starting centrifugation with programmed parameters 6 STOP diode is on of the moment of pressing the STOP key until the rotor is stopped 7 STOP key serves for centrifuging stopping Single time pressing will make stopping centrifuging with acceleration characteristics set in the software Pressing and holding it for 1s will make the centrifuging stop quicker During setting of the parameters it serves for exiting zones on the primary screen without introducing changes 8 UP key serves for the zone change on the display or for increasing the edited parameter 9 SET key serves for entry to the edition of parameters and confirming the set parameter 10 DOWN key serves for the zone change on the display or for reducing the edited parameter 17 7 2 Display The display is located in the centre of the control panel The main screen is presented below for MPW 380 and MPW 380R PROG 11761 PARA b CONFIG b 31 10 MPW 380R js 3 14 15 Td a PROG 11 161 e PROG 11761 2 PARA PORT jk CONFIG gt Wy 12 14 E IS 17 1 Assigned speed in rpm 2 Measured rotor speed in rpm 3 Assigned RCF 4 Measured RCF 5 Assigned value of the centrifug
10. be highlighted and at the same time the cursor will stop to blink Now with UP and DOWN keys it is possible to change the value of the chosen parameter indicated by the cursor It is necessary to confirm the change by pressing the SET key Shall no key be pressed within 20 seconds the device will automatically return to the main screen 34 Current date stored in the clock The tag of setting the parameter pointed by the cursor nee aon eae If it is highlighted it is possible to change the setting Current time The flickering cursor is pointing at the setting datum hours minutes seconds If it is highlighted it is possible to change the setting TIME DATE TELTE DATE ET 0092k 1 83 2888 1 AR EHF ours minutes seconds Day month year 7 6 5 CYCLES working time In the CYCLES menu the following statistics are displayed total working centrifugation time working cycles counter When the total working time is greater than 2000h the warning splash screen will be displayed every time the centrifuge is switched on In that case the centrifuge should be examined by the manufacturer s service CYCLES Warning message can be disabled In order to it follow the instructions below With the UP DOWN keys choose WARNING WORKING 2000 HOURS Press SET until x disappears Choose EXIT Note The CYCLE WARNING MESSAGE is turned on by default In this fold the total working
11. calculate with it the rotational speed to be set in the program of centrifuging Selection of the time of sedimentation and the RCF value shall be carried out experimentally for any given product Once every 100 rpm an electronic circuit automatically calculates and displays RCF value In order to program required RCF value one shall use nomograph Drawing No 5 or change the rotational speed matching displayed value to required acceleration value 7 12 2 Nomograph of relationship rotational speed centrifuging radius RCF Drawing No 5 7 12 3 Maximum load In order to avoid overloading of the rotor one shall observe maximum load which is recorded on every rotor Maximum permissible load is reached when all test tubes are filled with the fluid with 1 2 g cm density If density of the centrifuged liquid is higher than 1 2 g cm then test tubes could be filled only partially or one shall limit operation speed of the centrifuge which is being calculated from the formula ie oye G n perm n max y specific gravity gt Nmax maximum rotational speed rpm Y cm 50 7 13 Temperature in rotational chamber Centrifuge 1s equipped in ecological refrigerating system with temperature control During centrifugation there may appear differences in temperature on the display and temperature of the samples in the rotor It depends on thermal conductivity of the rotor and samples and centrifugation time In ord
12. calculated on the basis of the chosen number of characteristics 0 9 the assigned speed and chosen rotor In case of the user s characteristics numbers 10 19 it is total time of all sections of programmed deceleration characteristics Displayed in the format h min sec 9 Total time of the acceleration of centrifuge calculated on the basis of the chosen number of characteristics 0 9 the assigned speed and chosen rotor In case of the user s characteristics numbers 10 19 it is total time of all sections of programmed acceleration characteristics 10 Selection field 1f empty the function connected with it is disabled 7 5 2 Acceleration and Deceleration characteristics Characteristics of 0 9 numbers are linear and present the pace of changes of the rotational speed of the rotor per second Each rotor features acceleration and deceleration characteristics matched experimentally Characteristics of 10 19 numbers are set by the user as 1 2 3 or 4 segments characteristics It is being done through the edition of the user s saved program in the CURVES fold If a program with the user s characteristics 10 19 is loaded there is a possibility to change these characteristics to default ones from the range 0 9 and then return once again to the user s characteristics 28 7 5 3 Radius Control of the radius of the rotor within the range from Rmin to Rmax of the rotor The change of the radius of the rotor will result in displaying
13. characteristics will cause beginning of programming of the whole deceleration characteristics once again with settings of the program loaded to edition 7 6 11 Rotor Runtime preview of the quantity of the working cycles and the centrifuging time for each rotor The fold contains data of the time of centrifuging and of the quantity of the working cycles of each rotor The table also contains icons warning of the duty of execution of validation Scroll the table with UP and DOWN keys Exit the fold with the SET key 7 6 12 10 Cycles preview of spinning parameters of 10 last cycles The fold contains information concerning the parameters of ten last cycles of centrifuging Scroll the list with UP and DOWN keys Change the number of cycle with the SET key Exit the fold with the STOP key 44 7 6 13 Contact Us fold with data of the MPW company The fold contains information about the type of the centrifuge number of program and contact data of the MPW Company Marker informing on the available direction of the scrolling only down with DOWN key both up and down with UP DOWN keys only upwards with UP ke INFO MEDICAL CENTRIFUGE MPU 354k MPM MED INSTRUMENTS 4 34 1 MARSAN 46 BOREMLOWSKA St IHNTERHMET HTTP WHA MFH PL Exit the fold with the SET key Shall no key be pressed within 20 seconds the device will automatically return to the main screen 7 7 Starting of centrifuging Before be
14. corrosion one has to clean regularly the rotor with the fastening bolt buckets and round carriers Cleaning of the accessories shall be carried out outside of the centrifuge once every week or still better after each use Then those parts shall be dried using soft fabric or in the chamber drier at ca 50 C Especially prone to the corrosion are parts made of aluminium For cleaning them one should use neutral agent of pH value from 6 to 8 It is forbidden to use alkaline agent of pH above 8 In this way the useful service life of the device is substantially increased and susceptibility to corrosion is diminished Accurate maintenance increases the service life as well and protects against premature rotor failures Corrosion and damages resulting from insufficient maintenance could not be subject of claims lodged against the manufacturer 3 3 Lubrication The rotor pins shall be always lubricated with technical petroleum jelly In this way the uniform deflection of the buckets and quiet centrifuge operation is ensured 8 4 Glass tube cracking In the case of glass tube cracking all debris shall be accurately removed Rubber inserts shall be exactly cleaned or possibly replaced Otherwise one has to take into account following possibilities Glass particles left in the rubber cushion pad will cause once more glass cracking Glass particles left in containers make impossible uniform deflecting of the buckets and round carriers resulting in
15. internal error of the meter system error CRC error Launched RESET through Watchdog the inverter didn t answer the sent order during 30ms lack of byte the inverter answered NAK after sending the order inverter didn t put BETRIEBSBEREIT 1 in reply to FREIGABE 0 unidentified error of the RS485 broadcast during the reception and the verification of the code of the error from the inverter the driver didn t obtain one of bytes out of the inverter at the reception of the code of the error time limit 30 ms the attempt at reading the code of the error from the inverter didn t succeed wrong CRC unidentified error of the contact driver inverter 7 6 9 Reset deleting all programs the user settings and restoring of the default settings With UP and DOWN keys we choose the YES or NO option and confirm it with pressing the SET key Rese WARNING CONF TGURA HAD ORIGIN Choosing the yes option irreversibly deletes all programs and the user settings Default setting will be loaded Shall no key be pressed within 20 seconds the device will automatically return to the main screen ATTENTION In case of deleting all user programs when no 1 program is currently active default program please follow the instructions below before continuing enter the List of user programs ka select no 1 program gt press SET key choose DELETE option 38 7 6 10 Curves acceleration and deceleration curve
16. serial RS 232 pin 17151 Polycarbonate cap for bucket No 13275 17179 Aluminum cap for bucket No 13178 17792 Aluminum cap for bucket No 13792 17832 Polycarbonate cap for bucket No 13831 17834 Aluminum cap for bucket No 13833 CAUTION 4 4 manufactured for individual order 2 2 Exploitation materials For operating in centrifuge one should use only original company s buckets comprised in the NM specification of accessories as well as test tubes for centrifuges of proper diameter length and strength Utilization of test tubes of other makes shall be agreed upon with manufacturer of the centrifuge For cleaning and disinfecting one should use agents generally applied in the health service such as e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F 3 Installation 3 1 Unpacking the centrifuge Open the package Take out the cardboard box containing the accessories Take out the centrifuge from the package Keep the package and packing materials at hand for service transport 3 2 Location The centrifuge shall not be located near the radiators and shall not be subjected to direct sunlight ZA The table for the centrifuge shall be stable and shall have flat levelled table top It is necessary to ensure a safety zone of the minimum 30 cm round the centrifuge from every direction Normal operating conditions ambient temperature is from 15 C to 35 C Passed parameters of the centrifuge a
17. the rotor applied and in the case of its incompatibility with the type indicated in the application or absence of the rotor the spinning process shall be stopped with simultaneous displaying the error message see p 7 7 1 The conformity of the type of the rotor is signalled with a single audible signal 6 6 4 Rest state inspection Opening of the centrifuge s cover is possible only with the rotor in the state of rest When the rotor is being stopped the STOP diode is on and goes off when it is stopped 6 6 5 Checking of excessive temperature If temperature in rotation chamber exceeds 50 C caused by for example malfunction of cooling system drive will be switched off and error message will be displayed see p 7 7 1 The reboot is only possible after chilling the device 16 7 Description of the centrifuge operating elements Power switching ON OFF is carried out with master switch situated on left wall of the centrifuge All settings on the centrifuge are done by means of the control panel Panel comprises control keys display and signaling LED s I 7 1 Control panel The control panel placed on the front casing serves the purpose of controlling centrifuge operation MPW Centrifuge MPWESED MED INSTAUM ENTS SPEED RCF TIME COVER SHORT START 4 STOP u MPW 380R Drawing No 4 Control panel 1 COVER diode is on when the cover is open 2 COVER key serves for opening the cover 3
18. time in sec Number of the user s acceleration characteristic automatic numeration The EXIT field is marked at first the message is blinking Pressing the SET will cause the return to the CONFIG CURVES fold without making changes in the acceleration characteristics 39 A PROGRAMMING OF THE FIRST SECTION OF THE ACCELERATION CHARACTERISTICS To edit the acceleration characteristics one should mark the section of characteristics with UP or DOWN key the whole TIME SPEED line will begin to blink at this stage it is only a one section with the number 1 and then press the SET key The device will proceed to setting the characteristics section time only the set TIME value is blinking With UP and DOWN keys one should set the required time value and press the SET key The set time value has to be bigger than 0 After setting the time the device will proceed to setting the speed of the given section of characteristics only the set value SPEED blinks With UP and DOWN keys one should set the speed value and press the SET key The set speed value is limited by the maximum speed of the rotor connected with the edited program After the end of programming the speed the graphical displaying of the section of all sections will occur TIME SPEED of the user s acceleration characteristics B PROGRAMMING OF THE NEXT SECTIONS OF CHARACTERISTIC After programming the section I there is a possibility to program the next section number
19. time of centrifuge is being displayed hours minutes seconds and the number of working cycles Exit the fold by pressing the SET key or it by closed automatically after 20 sec If centrifuging will be stopped from any reasons within the set time such a cycle is not counted up 35 7 6 6 Rotating time parameters of the centrifuging time This fold enables the selection of the way of counting the set centrifuging time the time may be counted from the moment of pressing the START key or from the moment when the rotor reaches a set assigned speed itis possible to choose whether the time of centrifuging shall be the displayed increasing time count or the time remaining centrifuging time decreasing time count The selection takes place by pressing UP or DOWN key the marked parameter is blinking and pressing the SET key Choosing of the work parameter it indicated by checking on its field Shall no key be pressed within 20 seconds the device will automatically return to the main screen 7 6 7 Buzzer The fold makes it possible to switch on ON or switch off OFF short audible signals accompanying every pressing of any key Warning signals connected with error occurrence cannot be switched off Shall no key be pressed within 20 seconds the device will automatically return to the main screen The selection takes place with UP or DOWN key the marked option is blinking and the SET key The field of the chosen option will be c
20. to the main screen Marker of the chosen rotor Maximum speed of rotor 7 5 Para zone of setup of operations parameters Choose PARA zone press SET choose PARA zone with UP DOWN keys press SET The screen with parameters of centrifuging will appear During centrifuging it is possible to change the settings in following folds e ACCELERATION number of acceleration characteristic DECELERATION number of deceleration characteristic COOLING cooling of the centrifuge chamber OPEN LID AFTER RUN automatic opening of the cover after the centrifuging is finished START DELAY delayed START of centrifuging During centrifuging it is not possible to change the following settings e RADIUS rotor radius mm e DENSI sample density g cm Shall no key be pressed within 20 seconds the device will automatically return to the main screen 27 7 5 1 Description of the function in the Para fold PHRHME lt ea R ITA ih ID coef tii RATAJ LE lt a pounne u E M H L Fr Ii i a T TiTi Tia Tu a pi su num LL q MJ kc pa rT 1 Number of acceleration characteristic 2 Number of deceleration characteristic 3 Rotor radius mm 4 Sample density 5 COOLING cooling of the centrifuge chamber 6 Automatic opening of the cover after the centrifuging is finished 7 Delayed START of centrifuging 8 Total time of the deceleration of centrifuge
21. zones I range from 5 sec to 9 min 59 sec by 1 sec intervals Setting in this zone is marked by mark under min sec values mark of time zone 5sec 9min 59sec Minutes Seconds II range from 10 min to 9 h 59 min in 1 min interval Setting in this zone is marked by mark under h min values een mark of time zone 10min 9h 59min III range of continuous work of the centrifuge unlimited centrifuging time When setting the centrifuging time longer than 9 h 59 min instead of centrifuging time the HOLD message is being displayed It means that the centrifuge will be working until pressing the STOP key HOLD message means continuous centrifugin 22 7 4 4 Temp Temperature MZEE Setting the temperature within the range from 20 C to 40 C by 1 C interval reached temperature 13 C 40 C 7 4 5 Prog User s programs Range of programs from 1 to 99 The program chosen most recently is activated automatically when turning on the centrifuge If none program was chosen or in the case of a change of the parameter without saving the sign is being displayed It order to select the program choose the PROG zone press the SET key chose zone with UP or DOWN keys PROG message will begin to blink IME TEMP cc 99 1 press the SET key and proceed to the selection of the program number sign or program number w
22. 2 Se ME SAS REE 1 Bibel ll Za Programming of new section possible the whole line 2 1s blinking Programming as in the case of section 1 It is possible also to abandon the programming with UP DOWN keys choose the EXIT option it will blink and save press the SET only the acceleration characteristics of 1 section with TIME SPEED parameters described in the line 1 The minimal speed of the next section of acceleration characteristics is equal to the speed of the already programmed previous Section in the above mentioned case the programmed speed of section I 2000 rpm the lowest speed possible for programming of a section 2 2000 rpm 40 After programming the section 2 there is a possibility to program the next section number 3 Programming of new section possible the whole line 3 is blinking Programming as in the case of section 1 It is possible also to abandon the programming with UP DOWN keys choose the EXIT option it will blink and save press the SET only the acceleration characteristics of 2 section with TIME SPEED parameters described in the line 1 and 2 After programming the section 3 there is a possibility to program the next section number 4 The variability of the programmed acceleration characteristics comprising four sections Programming of new section possible the whole line 4 is blinking Programming as in the case of section 1 It is possible also to abandon the programming with UP DOWN k
23. 3 9 Emergency conditions service 9 1 Fault finding Majority of faults could be removed by switching the centrifuge OFF and then ON After switching the centrifuge ON there shall be displayed parameters of the recently implemented program and sound signals comprising four successive tones shall be generated In the case of short duration power failure the centrifuge terminates the cycle and displays PROGRAM ERROR code Please find below the most frequent faults and their repair methods MPW 380 R e Is supply cable plugged into mains ao a supply cable correctly e Js master e Is master switchON ON e Is master switthON Switch ON power Switch ON power supply e The above was checked and still there is no display Call service active and check sound audible e START key pressing does not generate reaction or single tone only START diode is on Wait till rotor stops and the START diode goes off COVER diode is on Close cover COVER diode must switch off dot on the display is blinking Centrifugation cycle in progress press STOP key or wait till cycle ends e Indications are proof for cycle in progress and Switch power supply OFF ON If fault still motor does not start persists call service e After stopping unbalance message is displayed Unequal rotor load Centrifuge load shall be balanced Inclined centrifuge Centrifuge shall be levelled Faulty drive mechanical damage Call service
24. Press SET With the UP DOWN keys set the valid 100s PASSWORD place of the PASSWORD e g 12xx 1234 Press SET With the UP DOWN keys set the valid 10s place of the PASSWORD e g 123x Press SET With the UP DOWN keys set the valid 1s place of the PASSWORD e g 1234 Press SET CODE CODE As a confirmation repeat instructions described above When the PASSWORD is set the Key sign is displayed in the CODE zone It is also displayed in the main menu lower right corner of the screen CODE To delete the PASSWORD 0000 must be set If the PASSWORD is forgotten the emergency code 7654 should be used to clear password and remove all locks 2 CODE locks With the UP DOWN keys choose a LOCKS lock meam Hi Ol CHANGE PARAMETERS Mark a lock by pressing SET Ol LOAD PROGRAM O START KEY the chosen lock will be marked with DISABLED no programs can be saved acceleration charactristics can not be SAVE PROGRAM Wis button saved deceleration charactristics can not be saved no programs can be deleted DELETE PROGRAM M3 button saving programs on position where one was already stored is disabled CHANGE PARAMETERS parameters can not be modified LOAD PROGRAM no programs can be called up START KEY centrifugation can not be started e PAS SHORT ARAA i Executing disabled procedures is only possibile after entering the PASSWORD Using LOCKS described a
25. RRENT PROTECTION it A A A iia aa 10 DESCRIPTION OF THE CENTRIFUGE cinc 11 AGE NERA DESCRIPTION OGON ka A O RA da E R OJO 11 SERVICE PLE MENOS SOA N etc AAA eee E A OO ean eae oe 11 SAFE WORKING CONDITIONS 2 A A A aa 12 SL SER VICENG PERSONNE Deco a a E A o e o oa 12 5 2 GUARANTEE AND OPERATIONAL USE PERIOD 0ccccccssssecccccesecceecussecceeauseeccecuueeecceeuuseeccesaueeeceesaueeeceeeauneeeeeeaueeeeees 12 SAR ER EE PING PERIOD PA RO OE EA W OOOO R Aa 12 54 IIINISON CENTRIGUGING OK ROK S 12 5 5 HAZARDS AND PRECAUTIONS 0er a sd aia ra ada ici 13 OBRERA TION OE THE CENTRIFUGE ts 14 6 1 MOUNTING OF THE ROTOR AND ACCESSORIES oo Ol ai aiii 14 6 2 CONSTRUCTION AND SAFETY MEASURES oai akira Od dawal kodowa deal 15 6 3 DRIV GSO NOOO OO dO OOO NOP 15 64 DATAINPUTAND OUTPUT caia iii iia 15 CON RI e O 15 6 0 SAFETY DEVICES 00 dais 16 COL COVEN TOC AAA OZ AO OE O OB 16 6 6 2 Unbalanced load CHECKING SYSTEM te Fees sie uses ca cere a aaa eens ee ee 16 6 6 3 Rotor installation and software compatibility verification unit ooonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannnnnnnncnnnnnnnnnn nono nn wawa aa 16 GOA AROSE SIQIE SPECON tO ASA OOOO O AOC ONAR O NA 16 6 6 5 Checking of excessive COMPETIR i WAWA A AGA 16 DESCRIPTION OF THE CENTRIFUGE OPERATING ELEMENTS 2200022222000000000000000000000000000000000000000000000000 17 ZASCONIROLPAN ELB GKRPA ai 17 RZEDISPEA oso II A N 18 kezd VAVUQULONS of RO MATES CCOO
26. TIONS OF THE ROTATING CHAMBER AND ACCESSORIES cccccccccccceeeeeeeeeeeeeennnneeeeees 53 9 EMERGENCY CONDITIONS SERVICE zicc ds kl zd s dzo w i GE GE 54 DL BAULTEINDIN Cdad dados 54 9 224 WORK SARE LY INSPECTION pi as oases EOG A 56 9 3 INSPECTION PROCEDURES CARRIED OUT BY THE OPERATOR ssseccecccceeeeeeeeeeeeeeeeennnnaaeeeeeeeeeeeeeeeeeeeeeeeeaaaaeeeees 56 10 REPAIR CONDITIONS eean eeaeee aeara enea aaeeea rre E Eear eeaeee a daer aee or ESNEA EER Eaa 57 TDS E AA O RTE 57 MANUFAC FURER S DATA arrian eiee EEEE EEEN EES EREE EE EC a Edo SOC 57 TS DISTRIBUTOR INFORMATION aieas eenia eeaeee PCE APE APRA 57 A ZR e ZN RZ ORO 58 13 1 NOMOGRA Ph cesi ORW w da ot es E A ee Eee 58 14 2 TABLE OF CHEMICAL RESISTANCE TO THE INTERACTION OF VARIOUS CATEGORIES OF REAGENTS OF PLASTICS 59 14 3 PRINTING OF CENTRIFUGATION PARAMETERS PROTOCOL THROUGH RS 232 PORT 1111111111111 60 Annexes Statement of conformity Declaration of decontamination repair Declaration of decontamination returning device 1 Application The MPW 380 R centrifuges are table top laboratory centrifuges for in vitro diagnostic IVD MPW 380R is equipped with cooled rotating chamber used to separation of samples took from people s animal s and plant s components with different densities under the influence of the centrifugal force to provide information about their biological state MPW 380
27. bove it is possible in any configuration 33 7 6 2 Language choice of the language Choice of the language option as regards messages displayed by the driver With UP and DOWN keys check the demanded option the chosen language version is blinking and press the SET key The selection field by the chosen language version will be checked Shall no key be pressed within 20 seconds the device will automatically return to the main screen Exit field anguage version selection field 7 6 3 Screen choice of the mode of the basic screen 3 modes of the main screen operation may be selected see p 7 2 1 e displaying SPEED RCF standard digits on LCD e displaying only SPEED increased digits e displaying only RCF increased digits Options are set with UP DOWN keys and the SET key Shall no key be pressed within 20 seconds the device will automatically return to the main screen 7 6 4 Time Date setting the time and date The driver is fitted with the real time clock RTC with battery support TIME DATE fold serves the purpose of setting the current time and date In the S RAM clock memory there is also information stored on the quantity of centrifuging cycles and the total time of work of the centrifuge In order to set the time use UP and DOWN keys to set the blinking cursor by the datum which is to be changed hours minutes seconds day month year and press the SET key The SET message on the display will
28. cket 4 Centrifuge accessories and especially structural changes corrosion preliminary cracks abrasion of metal parts 5 Screw joints 6 Inspection of the rotor assembly 7 Inspection of bioseals of the buckets if such are used 8 Control of execution of the guarantee yearly technical inspection of the centrifuge Only the manufacturer specified holders included in the equipment list as well as centrifuge capillaries which diameter length and durability are suitable should be used for spinning in this centrifuge The use of equipment made by other manufacturers should be consulted with the manufacturer of the centrifuge In the centrifuge disinfectants and cleaning agents generally used in medical care should be used e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F 56 10 Repair conditions Manufacturer grants to the Buyer the guarantee on conditions specified in the Guarantee Certificate Buyer forfeits the right to guarantee repair when using the device inconsistently with the User manual provisions when damage results from the User s fault or when the Guarantee Certificate was lost Repairs should be carried out in authorized service workshops granted with the MPW Certificate The centrifuge shall be sent to repair after decontaminating disinfections Information about authorized service workshops could be obtained from the Manufacturer 11 Disposal When you are disposing the
29. cket 13833 Round carrier 84 mm for 200 ml bottle b 57x113 mm for bucket 13833 Round carrier b 38 mm for 50 ml Falcon test tubes 30x120 mm for rotor 11778 Round carrier b 38 mm for 15 ml Falcon test tubes b 17x120 mm for rotor 11778 Round carrier 84 for test tubes 3x50 ml 35x100 mm for bucket 13833 Round carrier 83x95 for test tubes 28x1 2 ml S Monovette 9x82 3 mm for bucket 13831 Round carrier 83x95 for test tubes 25x5 ml b 13 1x100 mm for bucket 13831 Round carrier 38 for test tubes 24x1 2 ml S Monovette 9x82 3 mm for bucket 13833 Specification Polypropylene round bottom test tube 2 ml with cap 10 8x40 mm Polypropylene test tube 1 2 ml s Monovette 0 8 8 13 3x82 3 mm Polycarbonate round bottom bottle 250 ml 962x122 mm Polypropylene test tube 2 ml with cap 9 10 8x41 2 mm Polypropylene test tube 100 ml with cap 6 44 5x103 mm Polypropylene test tube 14 ml with cap 0 16 8x113 mm Polypropylene test tube 15 ml Nalgene 6 16x113 mm Polypropylene test tube 15 ml Falcon 17 21x120 mm Polypropylene test tube 50 ml Nalgene 28 8x106 7 mm Polypropylene test tube 50 ml Falcon 30 35x120 mm Polypropylene test tube 10 ml with cap 16 19x100 mm Polypropylene test tube 6 ml with cap 11 5x92 mm Polypropylene test tube 30 ml with cap 9 24 8x100 mm Polycarbonate test tube 30 ml Nalgene with cap b 25 5x94 mm Polycarbonate test tube 85 ml Nalgene with cap 0 37 8x106 mm M
30. device the respective statutory rules must be observed Pursuant to guideline 2002 96 EC WEEE all devices supplied after August 13 2005 may not be disposed as part of domestic waste The device belongs to 8 group medical devices and is categorized in business to business field The icon of the crossed out trash can shows that the device may not be disposed as part of domestic waste The waste disposal guidelines of the individual EC countries might vary If necessary contact your supplier 12 Manufacturer s data MPW Med instruments 46 Boremlowska Street 04 347 Warsaw Poland tel 48 22 61056 67 sales department 48 22 610 81 07 service fax 48 22 610 55 36 e mail mpwOmpw pl www mpw pl 13 Distributor information DISTRIBUTOR 57 14 Appendix 14 1 Nomograph a r p m Centrifuging radius cm R C F x g 50 multiple of 20000 gravitational acceleration 45 15000 100000 14000 40 13000 Formula used for calculation of this 12000 nomogram 50000 11000 2 40000 1 ee R C F 11 18 r n 1000 oe 30000 9000 where m 20000 RAE R C F multiple of 7000 T gravitational speed i 97 r centrifuging radius cm 10000 6000 n rotational speed r p m gt itational accelerati 8000 B 25 g gravitational acceleration 5000 24 6000 22 4006 C 4000 21 3000 3500 20 2400 a 2000 3000 1500 18 1200 2500 17 1000 19 2000 1 600 500 400 lA 1500 13 300 1400 1300 10 200 1200 Example
31. e load or delete a user program one should use UP and DOWN keys to set the indication marker by the chosen number on the list and then press the SET key A selection window will appear example operations on the number 5 on the list Number of the program from the list Exit from the list of the programs whom executed operations refer to without making any operations Field of the deleting the program and exit from the list Field of the loading the progr a and exit Field of the saving the program from the list and exit from the list Field of the canceling of the operation enable the return to the list of programs 7 4 7 Program of preliminary refrigerating Special program no It is a program intended for preliminary cooling of rotors irrespective of the kind of a rotor Centrifuging operates with parameters SPEED 2500 rpm TIME 30 min TEMP 4 C The program is marked with the padlock icon which means that it is not possible to modify it or to save it if it was loaded Additionally after loading this program any changes in zones SPEED RCF it is not calculated in this section 0 is being displayed TIME TEMP ROTOR_LIST PARA will be blocked Blocked access to these zones is signalled with padlock icons O ee padlock icon is signalling is not displayed that there is no access to this If program no 1 is loaded then in order to remove it from driver memory it is necessary to load other program
32. e increasing or reducing time by the choice gt selecting acceleration characteristics from O quickest to 9 slowest with displaying the time in s gt selecting deceleration characteristics from 0 quickest to 8 slowest deceleration characteristics number 9 is subterfuge characteristics with time of free stopping of the rotor in s gt programming of optional acceleration and deceleration time gt programming of multi segment characteristics of the working cycle with graphical visualisation gt setting the rotation chamber temperature 20 40 C reached temperature 13 C 40 C MPW 380R gt selection of the COOLING option cooling the rotation chamber after the end of centrifuging MPW 380R gt selection of the program no 1 cooling the rotation chamber and accessories before start of centrifuging gt selecting the AUTO COVER function automatic opening of the cover after centrifuging is finished gt setting the START DELAY option time settings gt setting the RADIUS option changing of rotor radius mm setting the DENSITY option changing of sample density g cm 15 gt setting the configuration menu access code gt selection of menu language polish english spanish gt selection of the primary screen mode display SPEED RCF or SPEED only or RCF only gt setting the DATE and TIME gt preview of spinning parameters of 10 last cyc
33. e message is blinking Pressing the SET key will cause returning to the CONFIG CURVES fold without making changes in the deceleration characteristics A PROGRAMMING OF THE FIRST SECTION OF THE DECELERATION CHARACTERISTIC To edit the deceleration characteristics one should mark the section of characteristics with UP or DOWN key the whole TIME SPEED line will begin to blink at this stage it is only one section with the number 1 and then press the SET key The device will proceed to setting the characteristics section time only the set TIME value is blinking With UP and DOWN keys one should set the required time value and press the SET key The set time value has to be bigger than 0 After setting the time the device will proceed to setting the speed of the given section of characteristics only the set value SPEED blinks With UP and DOWN keys one should set the speed value and press the SET key The set speed value cannot be higher than the initial speed of the rotor at the moment of beginning of deceleration the set speed of centrifuging In order to compete the creation of the deceleration curve it is necessary for the speed of the last of programmed sections of the curve to be equal 0 Otherwise the curves wizard will not enable the end of programming it will be impossible to select the EXIT option 42 B PROGRAMMING OF THE NEXT SECTIONS OF CHARACTERISTIC After programming the section 1 there 1s a possibility to prog
34. e setup from 0 for ambient temperature PARAME TEE ACCELERATIC DECELERA PARA EJ RADIUS DENS I Time ALL Time IEL saseste Po pie i M A wocests zzee a Lu IL EA kM FE a iaar BALRAJ os z I qa enla PATA LTE The COOLING function is operating in the following way the temperature set on the PARA fold of the COOLING function is being stabilized during the stoppage of the centrifuge It is being signalled with the icon C on the main screen Opening the cover switches the compressor off Closing thereof enables stabilization of the temperature again After starting the centrifuging the device proceeds to the stabilization of temperature set in the TEMP zone within range 20 to 40 C Icon displayed when COOLING function is enabled Temperature of the COOLING function set in the PARA fold PEEL RCF TERE R EHF Dy A I l PROG If the COOLING function is checked off then during the centrifuge s stoppage the access to settings in the TEMP zone on the main screen is blocked the set temperature with COOLING function is displayed here 30 7 5 6 Open lid after run Automatic opening of the cover after the centrifuging is finished Checking on this function will cause automatic opening of the cover if the centrifuging cycle proceeded without interferences the full centrifuging time was counted down In the case of early stopping of the centrifuge pr
35. ed value Turning on the compressor and starting the cooling is signalled with the icon on the display After switching the compressor off one has to wait 15 seconds before switching it on again Mark by the switching the compressor on coolin 7 7 3 Screens informing about the end of centrifuging Centrifuging may be stopped at the any moment with the STOP key The information message is being displayed then After pressing the SET key the device returns to the main screen Stopping centrifuging in accordance with the set time causes generating 3 short audible signals after stopping the rotor and displaying the message After pressing the SET key the device returns to the main screen 48 7 7 4 Additional messages 1 In the case of power shortage while centrifuging after repeated switching it on the following error screen will be displayed After pressing the SET key the message is permanently deleted 2 After operating for 2000 hours after every switching on the centrifuge the error screen is being displayed with information about the necessity to carry out servicing activities After pressing the SET key the device proceeds to the main screen and the device may operate WORE IMG CHLL SE VALIDAT This message cannot be deleted by pressing the SET key Only the manufacture s service of the centrifuge is able to delete it 7 8 Change of settings in zones 1 CENTRIFUGE IS NOT WORKING e Program no l
36. ed on CD Centrifuging and report printing Begin centrifuging process Complete report is displayed on the PC screen and can be saved on a hard drive printed or opened with compatible windows application 60 Kawka printer Components needed to connect printer name quantity pcs cat no thermal printer KAWKA RS232 cable 16595 16593 Actions Connect printer to the source of power according to the instructions enclosed in user manual connection diagram is given below Connect centrifuge with a printer using delivered data transmission cable 16593 Press START button begin centrifuging When centrifuging process Is finished report will be printed Printout example 82 MIN 88 SEC CZAS LICZONY OD NACI NI CIA START 82 MIN 88 SEC 11771 2009 348 28 C 2008 19 08 87 Cac cz a r a a x a gt lt gt oc _ Ch ka ROZRUCHU 68 00 28 Ch ka HAMOWANIA 68 08 22 TEMPERATURA PR DKO TEMPERATURA KONIEC CYKLU WIROWANIA CZAS START CYKLU WIROWANIA PARAMETRY OSI GNI TE DANE PROGRANU 2088 81 81 MATERIA OPERATOR RAPORT NR Centrifuging date and time are listed in the report u Date and time are set by the manufacturer for the local area u In case of printing incorrect date time one should connect printer to the PC and set current date time using delivered application 61 Connection diagram CENTRIFUGE Ta
37. ent steam sterilization reduces mechanical durability PC test tubes may become useless 2 Except PVC hoses which are resistant to the steam sterilization in the temperature 121 C Abbreviations of names of characterized plastics PS Polystyrene ECTFE Ethylene chlorotrifluoroethylene SAN Styrene acrylonitrile ETFE Ethylene tetrafluoroethylene PMMA Polymethyl methacrylate PTFE Polytetrafluoroethylene PC Polycarbonate FEP Tetrafluoroethylene perfluoropropylene PVC Polyvinyl chloride PFA Tetrafluoroethylene perfluoroalkylvinylether POM Acetal polyoxymethylenel FKM Fluorcarbon rubber PE LD Low density polyethylene EPDM Ethylene propylene diene PE HD High density polyethylene NR Natural rubber PP Polypropylene SI Silicon rubber PMP Polymethylpentene For centrifuging infectious materials it is necessary to use hermetically closed buckets in order to prevent they migration into the centrifuge Rotors buckets and round carriers can be sterilized in autoclave with temperature 121 124 C and pressure 215 kPa during 20 min In the centrifuge disinfectants and cleaning agents generally used in medical care should be used e g Aerodesina 2000 Lysoformin 3000 Melseptol Melsept SF Sanepidex Cutasept F User is responsible for proper disinfections of the centrifuge if some dangerous material was ZA spilled inside or outside of the centrifuge During the above mentioned works one must wear safety gloves 5
38. er to centrifuge preparations of a lowered temperature deposited in the outside refrigerator it is necessary to make initial refrigerating of the chamber the rotor and containers to the set low temperature in order to minimise the difference of temperatures In order to obtain lower temperatures of the chamber we recommend with centrifuging loading the program I In centrifuge there spin rotors with maximum speed 2500 rpm in 30 min obtaining the temperature C It is possible to cool the centrifuge chamber with the rotor stopped standby mode cooling To do so one should activate the COOLING function in the PARA fold see p 7 5 5 and set the desired temperature within range from 0 C to the ambient temperature The function activates automatically after confirmation and with the cover closed Opening the cover switches the cooling process off when the cover is closed again the function is reactivated again If the COOLING function is ON during the centrifuging cycle after the cycle ends the device switches into cooling regime with centrifuging OFF until the cover gets open Cooling of the centrifuging chamber also takes place with centrifuging in SHORT function The cooling system allows for cooling every available rotor with equipment with maximum speed up to 4 C Lower temperatures can be obtained when taking into consideration the type of rotor and speed 51 8 Cleaning disinfection maintenance CAUTION It is necessary t
39. es 17798 Complete clamp pcs 1 17799 Spanner for the rotor pcs 1 17642 Spanner for emergency opening of the cover pcs 1 17009 Power cord 230 V pcs 1 or 17010 Power cord 115 V optionally pcs 1 20380 R ENG User manual pcs 1 2 1 2 Optional accessories Depending on customer s needs the MPW 380 R centrifuge can be provided with below specified accessories ANGLE ROTORS Cat No Type of rotor Angle Rotor capacity ana RCF rem 11761 Angle rotor HSL 45 30 x 2 0 1 5 ml 16400 29168 9 7 6 5 11762 Angle rotor HSL 45 36 x 2 0 1 5 ml 18000 30065 8 3 5 0 11763 Angle rotor HSL 45 48x2 0 1 5 ml 16400 28265 94 6 2 11765 Angle rotor HSL 45 12 x 8x 0 2 ml PCR strip 16400 28566 9 5 8 4 11766 Angle rotor HSL 45 12 x 10 ml 81 mm 18000 31150 8 6 5 0 11767 Angle rotor HSL 30 12 x 10 ml 100 mm 14000 20598 9 4 5 0 11769 Angle rotor HSL 45 60 x 2 0 1 5 ml 16400 29168 9 7 6 5 11770 Angle rotor HSL 30 12 x 15 ml Falcon 14000 23227 10 6 4 6 11772 Angle rotor HSL 30 8x30 ml Nalgene 25x98 17500 29787 8 7 3 2 11773 Angle rotor HSL 30 6x50mlNalgene 28x106 14500 21625 9 2 3 0 11775 Angle rotor HSL 30 8x50ml Nalgene 14000 21255 9 7 5 0 11776 Angle rotor HSL 30 8x50ml Falcon 14000 23666 10 8 5 0 11777 Angle rotor HSL 25 4x250ml 10000 14086 126 5 7 11778 Angle rotor HSL 30 6 x 94 85 ml Nalgene 12000 17710 11 0 4 0 11779 Angle rotor HSL 45 36x2 0 ml Filter 15000 22136 88 5 0 11780 Angle rotor 30 12 x 50 ml Falcon 4500 3328 14 7 8 3 11784 Angle roto
40. essing the STOP SHORT keys or emergency switch off due to enabling the secure mechanisms the cover will remain closed and it will be possible to open it only with the COVER key 7 5 7 Start Delay delayed start of centrifuging If this function is checked on then after pressing the START key will the set time will be counted down and then the centrifuging will be launching Time range from 0 without start delaying to 9 hours and 59 minutes Waiting for counting down the time of the delay is signalled by the blink of START diode similarly as in the case of the COOLING function It is possible to abandon counting at the any moment by pressing the STOP key Delay time in the format h min sec Setting by UP and DOWN keys 7 6 Config configuration menu of the centrifuge Upon choosing the CONFIG zone and confirmation with the SET key the CONFIGURATION menu will be displayed With UP and DOWN keys one may choose the fold blinking message and with the SET key confirm beginning of the configuration of selected fold aa in the CONFIGURATION menu 31 7 6 1 Password locks Setting or changing the PASSWORD To prevent from an unauthorized use a PASSWORD can be set Note No PASSWORD is set by default The PASSWORD can be set as follows when the rotor is at a standstill Press the UP DOWN keys until PASSWORD blinks Press SET With the UP DOWN keys set the valid 1000s place of the PASSWORD e g 1xxx
41. exceed load limit set by the manufacturer Rotors are intended for fluids of average homogeneous density equal to 1 2 g cm or smaller when centrifugation is carried out at maximum speed When fluids of higher density shall be used then it is necessary to enter the value in the PARA fold See point 7 5 4 Density and also point 7 12 3 Maximum load e It is not allowed to use the rotors and round carriers with signs of corrosion or other mechanical defects e It is not allowed to centrifuge highly corrosive substances which may cause material impairment and lower mechanical properties of rotor and round carriers e It isn t allowed to use rotors and accessories not admitted by the manufacturer Let to use commercial glass and plastic test tubes which are destined to centrifuging in this laboratory centrifuge One should absolutely not use poor quality elements Cracking of glass vessels and test tubes could result in dangerous vibration of the centrifuge e It is not allowed to carry out centrifugation with the rotor caps taken off or not driven tight ZA e It is not allowed to lift or shift the centrifuge during operation and rest on it e It is not allowed to stay in the safety zone within 30 cm distance around the centrifuge neither leave within this zone some things e g glass vessels e It is not allowed to put any objects on the centrifuge 6 Operation of the centrifuge 6 1 Mounting of the rotor and accessories
42. eys choose the EXIT option it will blink and save press the SET only the acceleration characteristics of 3 section with TIME SPEED parameters described in the line 1 2 and 3 The speed of the last of programmed sections of acceleration characteristics overwrites the speed set in the edited program Repeated attempt to program already programmed sections of the acceleration characteristics will cause beginning of programming of the whole acceleration characteristics once again with settings of the program loaded to edition 41 Modification of deceleration characteristics Decel After choosing this option the window of the characteristics wizard is displayed Default deceleration characteristics connected with the loaded program will be displayed on the screen Creating of deceleration characteristics takes place a little differently than acceleration characteristics Centrifuging speed final in rpm for a given section of characteristics Number of section the characteristics of deceleration Initial rotor speed rpm at the moment of Time in sec after which th inni 7 ime in sec after which the beginning of deceleration centrifuging speed will be reduced to the SPEED value ioe Sst ME 9 SFEEL W Y 1 Hid z4 5 rbd El Return to the CONFIG fold Total acceleration time in sec Number of the user deceleration characteristic automatic numeration In the first moment the EXIT field is marked th
43. fa RS232 gt USB CONVERTER USB cable RS232 cable PRINTER data transmission cable 62 gw tuv o ZY Tel 48 22 673 04 08 22 879 70 46 z Management p Pr Fax 48 22 610 55 36 22 610 81 01 4 TUVRheinland e DIN EN ISO 9001 E mail mpw mpw pl www mpw pl e ZY e DINEN ISO 13485 o 0000023 No 10 380 03 ENG We MPW Med instruments Sp dzielnia Pracy 46 Boremlowska Street 04 34 Warsaw Poland PN EN 61326 2 6 000 PN EN ore No PN EN 13612 2006 PN EN 980 2010 PN EN ISO 18113 3 2011 M p W Med instruments SP DZIELNIA PRACY 1 w Warszawie STATEMENT OF CONFORMITY Warszawa 15 03 2012 gw tuv o ZY Tel 48 22 673 04 08 22 879 70 46 z Management p Pr Fax 48 22 610 55 36 22 610 81 01 4 TUVRheinland e DIN EN ISO 9001 E mail mpw mpw pl www mpw pl e ZY e DINEN ISO 13485 o 0000023 No 10 380R 03 ENG We MPW Med instruments Sp dzielnia Pracy 46 Boremlowska Street 04 34 Warsaw Poland PN EN 61326 2 6 000 PN EN ore No PN EN 13612 2006 PN EN 980 2010 PN EN ISO 18113 3 2011 M p W Med instruments SP DZIELNIA PRACY 1 w Warszawie STATEMENT OF CONFORMITY Warszawa 15 03 2012 DECLARATION OF DECONTAMINATION In order to protect our employees please fill out the declaration of decontamination completely before sending centrifuge to
44. for 2 glass test tubes 50ml for rotor 12786 13286 Bucket 85x130x60 mm for 2 5 microtiter plates and block for rotor 12787 13789 Bucket 85x130x60 mm for 1 4 microtiter plates and block for rotor 12788 13792c Bucket for ASTM with cap 17792 for rotor 12791 13831c Rectangular bucket with cap 17832 for rotor 12830 13833c Round bucket 400 ml with cap 17834 for rotor 12830 13865 Bucket for 7 test tubes 50 ml Falcon for rotor 12830 13866 Bucket for 6 test tubes 50 ml Falcon for rotor 12830 ROUND CARRIERS Catalog no Application 14000 Adapter 20x0 4 ml for rotor 12768 4 14002 Adapter 10x2 0 1 5 ml for rotor 12768 14017 Pad for 250 ml round bottom bottles for rotor 12177 and buckets 13174 13178 14036 Round carrier 028 5 special test tube ml 613 5x92 mm for rotor 11469 11773 11775 14043 Round carrier 029 for test tubes 5 ml 13x85 for rotor 11457 11776 14047 Round carrier b 16 8 for test tubes 5 ml 13x85 for rotor 11466 11770 14071 Round carrier for 30 ml test tubes nr 15055 25x100 mm for rotor 11776 14082 Round carrier 17 6 for 7 5 ml test tubes b 13 3x100 mm for bucket 13080 and 13081 14084 Round carrier 011 0 for 0 5 ml test tubes b 8 0x30 mm for rotor 11761 11762 11763 14089 Round carrier 29 0 for 15 ml Falcon test tubes 17x120 mm for rotor 11780 and bucket 13275 13276 14126 Round carrier 011 for 0 4 ml test tubes 05 8x46 mm for rotor 11761 11762 11763 14133 Round carrier 010 8 for 0 2 ml test tubes b6 2x21
45. ginning the centrifuging one should put on the rotor close the cover and program parameters of centrifuging particularly e to program the rotor of the number consistent with the assumed rotor e to program the speed centrifuging time and the set temperature Press the START key The fan of the engine is switched on and the process of identification of the rotor is being started SPEED RCF m TEMP 1 O sibtiss b LEFTLARI AAA IIA GETISSIESILISSESLISTS ESET LL PROG 5 al ii7T e6 PARA b CONFIG A Identification speed 85 rpm padlock icon signalling the ban Empty arrow Number of the chosen rotor 24 on the access means limited to the given zone access to settings during centrifuging while filled one unlimited access Marker informing that identificatio of the rotor is lasting Identification of the rotor lasts up to 6 seconds Shall the read number of the installed rotor is compliant with the chosen rotor the device will accelerate the rotor up to the set speed 45 Otherwise centrifuging is stopped and the error screen is being displayed Message about the reason of the stoppage in work of the centrifuge INCORRECT ROTOR HUMBER ID 11 r66 Identified number of the installed rotor If the rotor is unknown the sign is being displayed 7 7 1 Errors screens An irregularity in operations of the centrifuge is signalled by displaying a screen with relevant message a sound signal
46. hecked on 7 6 8 Sensor diagnostics of sensors and components errors The fold is intended for the manufacturer s servicing in case errors in operations of the centrifuge occur The state of the variable containing information about errors arisen in working of the driver sensors and components connected with the driver is presented here Data from this fold are saved in the durable memory until showing up of a new error Then they will be updated overwritten In any moment it is possible to delete the contents of the fold For this purpose one should choose the DELETE field with the UP key blinking when marked and to confirm by pressing the SET key To exit the fold press the SET key when EXIT field is blinking 36 Shall no key be pressed within 20 seconds the device will automatically return to the main screen Exemplary SENSOR screen Number of the component in the centrifuge Tag showing that the error connected with a device of a given number occurred in this case 0 SENSOR 15 14 is e Moro INU 2535 5_TEMP 6 DELETE EXIT Code of the error of inverter Code of the error of the temperature sensor DS18B20 Device No type of the error 0 error of the temperature sensor TEMPO connector X2 1 error of the temperature sensor TEMP1 connector X6 2 error of the temperature sensor TEMP2 connector X8 reserve 3 error of the inverter calling with signal BETRIEBSBEREIT 1 4 overheating of the slowing dow
47. ic opening of the cover after the centrifuging is finished 2 1111111111 31 7 5 7 Start Delay delayed start of centrifuging eee een aaa nana nn nnnncncnnnnnnnnnnns 31 7 6 CONFIG CONFIGURATION MENU OF THE CENTRIFUGE ccceeeeeeeseennccecececeeeeeeeeeeseeeeesnnnaneeeeeeeeeeeeeeeeeeeeeeeeaaaeeeees 31 Te OA NC ODE EA EA A W A NO E T 32 70 25 Language choice of the TAN GUAGE aca 34 7 6 3 Screen choice of the mode of the basic screen eeeeeeea aaa aaa aaa aaa aaa aaa aaa aaa i iii 34 7 6 4 Time Date setting the time and date uee aaa aaa aaa aaa aaa aaa aaa ni EEA 34 TOS PI O E O 35 7 6 6 Rotating time parameters of the centrifuging tlIMe cccccccccccccc ccc c cece aaa aaa aaa aaa wawa iii 36 ETS O ad l sere RG ens aR Gor i Tat te a A ta ease A tend BER kota S 36 7 6 8 Sensor diagnostics of sensors and components errors ccccccccccccccccc cece ee aaa eee aaa aaa wawa eeseeeeeeeeeeeeeeeaaaaaas 36 7 6 9 Reset deleting all programs the user settings and restoring of the default settings cccccccccccccccseeeeeeeeees 36 7 6 10 Curves acceleration and deceleration curveS eeeeeeeea aaa aaa aaa aaa aaa aaa aaa aaa aaa ae eeeeeeeeeeeeeeeneaaaas 39 7 6 11 Rotor Runtime preview of the quantity of the working cycles and the centrifuging time for each rotor 44
48. icro titer plate with cap 85 5x127 mm Glass tube 100 ml 45x100 mm 15116 Glass tube 50 ml 35x100 mm 15118 Glass tube 10 ml 16x100 mm 15119 Glass tube 7 ml 12x100 mm 15120 Glass tube 5 ml 12x75 mm 15121 Polypropylene test tube 10 ml with stopper 17x70 mm 15122 Polypropylene PCR test tube 8x0 2 ml 6x21 mm 15124 Polypropylene test tube 0 4 ml 5 7x46 mm 15125 Polypropylene test tube 0 2 ml PCR 6x21 mm 15127 Polypropylene test tube 0 5 ml with cap b 7 8x30 mm 15128 Polypropylene test tube 1 5 ml with cap 4 10 8x39 mm 15130 Polypropylene PCR test tube 8x0 2 ml 6x21 mm 15131 Polypropylene PCR test tube 4x0 2 ml 6x21 mm 15175 Polypropylene bottle 250 ml Herolab 9 61 5x125 mm 15176 Polycarbonate bottle 250 ml Herolab 61 5x135 mm 15419 Polypropylene test tube 5 ml 4 12x75 mm 15424 Polypropylene test tube 30 ml Nalgene with cap 25 5x94 mm 15440 Polypropylene bottle 200 ml 56 5x113 mm 15796 Glass centrifugal vessel ASTM cylindrical in the lower part conical lengths 167 mm BRAND no 3623 38 15797 Glass centrifugal vessel ASTM in the shape of the pear in the lower part conical lengths 160 mm BRAND no 3621 38 15852 Polypropylene bottle 400 ml 84x135 mm OTHER ACCESSORIES Catalog no Specification 16412 Locker under centrifuge 4 16594 Data recording set of working parameters by serial RS 232 pin 4 16595 Thermal printer of working parameters by
49. ill begin to blink With UP or DOWN keys choose the program number LME A ENE PRUG 6 Blinking program number Choose with UP and DOWN keys e finish setting the program number with the SET key The only possibility to stop of the program used recently is removing it from the list of programs see p 7 4 6 or setting another program 23 7 4 6 List of the user programs The centrifuging parameters SPEED RCF TIME TEMP acceleration characteristics deceleration characteristics and rotor number may be saved and then loaded by the user saving as one of 99 available programs In order to browse the list of programs choose the PROG zone press the SET key chose zone with UP or DOWN keys cursor of PROGRAM LIST will begin to blink a TEMF PROG 11761 Blinking cursor of PROGRAM LIST press the SET key and proceed to browse the list of programs Marker of the loaded program number Centrifuging time Rotor number Program number Deceleration characteristic Set speed Acceleration characteristic ate ete a Ma IRUHLS Tel FEE MEC FIA KL Marker of selected program Set temperature ATTENTION In case of deleting all user programs when no 1 program is currently active default program please follow the instructions below before continuing enter the List of user programs wa select no 1 program press SET key choose DELETE option gt 24 e In order the sav
50. ing time h min sec 6 Zone mark of assigning of the centrifuging time ZONE 1 5 sec 9 min 59 sec at 1 sec intervals ZONE 2 10 min 9 h 59 min at I min intervals 7 Centrifuging time cursor 7 or time remaining to end of centrifuging cursor 8 Cursor of counting of the centrifuging time 1 displaying the centrifuging time counting upwards displaying the time remaining to end of centrifuging 9 Assigned temperature MIES 20 C do 40 C 10 Measured temperature ANAIS 18 11 Number of the user s program 12 List of the user s programmes 13 Number of the rotor Number of the bucket 14 List of the rotors and buckets 15 Setting of work parameters of the centrifuge 16 Cursor of counting of the centrifuging time the flashing dots mean counting pending 17 Configuration menu 7 2 1 Variations of the main screen Beside displaying the mode SPEED RCF there is a possibility to set 2 extra modes of work displaying only SPEED or only RCF Switching to the suitable mode takes place on the CONFIG SCREEN fold MPW 380 MPW 380R TIME HM TEMP 2 00 20 PORG b CONFIG b 16 120 i lu PROG 6 66 7 2 PARA CONFIG gt only SPEED only RCF 7 3 Setting up parameters On the main screen it is possible to set e SPEED e RCF e TIME Temperature TEMP AMERO Selection of user s program PROG Selection of user s program from list programmes browsing
51. is also emitted The error screen is displayed until pressing the SET key All important errors are stored and can be reviewed in CONFIG SENSOR fold see p 7 6 8 As the display is capable of containing only one message at a time the following priority of importance of the displayed data applies 1 error of driver inverter communication Error of the I2C connection Error of the measurement of the temperature Overheating motor Error of inverter Rotor unbalancing ALAN EROS Overheating of the slowing down resistor Cover opened during centrifuging 9 pressure control actuation 10 measurement of the temperature 1s out of operation 11 speed during identification of the rotor other than 85 rpm 12 overheating of the centrifuge above 50 C Displayed error messages are as follows ot le eae u IMBALANCE FAST STOP PLEASE REMOVE CAUSE THEN RESTART OVERHEATING MOTOR OVERHEATING BRAKING RESISTOR OPENING COVER in RUN PRESSURE CONTROL ACTUATION TEMPERATURE SENSOR ERROR MPW 380R ERROR code of error INVERTER ERROR ERROR code of error SPEED METER ERROR 46 SPEED OF ROTOR IDENTIFICATION lt gt 85 RPM I2C BUS ERROR INVERTER SERIAL BUS ERROR ERROR code of error NO ROTOR INCORRECT ROTOR NUMBER ROTOR STOPPING Please wait 47 7 7 2 Stabilization of the temperature of the centrifuge Stabilization of the temperature of the centrifuge takes place with 1 C accuracy round the assign
52. les gt preview of the total working time and quantity of the full working performed cycles gt preview of the quantity of the working cycles and the centrifuging time for each rotors gt possibility to switch the key signals on off gt possibility to register the centrifuging parameters by connecting the centrifuge to a PC with a printer or Kawka thermal printer gt displaying information on emergency states 6 6 Safety devices Apart from the above described passive devices and safety measures there exist as well active devices and elements as follows 6 6 1 Cover lock The centrifuge can be started only with properly closed cover the COVER diode is not shining While the cover can be opened only after stopping the rotor In the case of emergency opening of the cover during operation the centrifuge will be immediately switched off and the rotor will brake till complete stopping With opened cover the COVER diode is shining the drive is completely disconnected from the power which makes it impossible to start the centrifuge 6 6 2 Unbalanced load checking system When loads of opposite buckets or carriers in rotors are unbalanced the drive will be switched off during acceleration or operation of the centrifuge and the error message will be displayed see p 7 7 1 6 6 3 Rotor installation and software compatibility verification unit Directly after starting centrifuging a unit verifies the type of
53. m for bucket 13275 and 13276 Round carrier 83x95 mm test tubes 13x15 ml Falcon 17x120 mm for bucket 13831 Round carrier for ASTM laboratory ware of 160 mm lengths 15797 Round carrier for ASTM laboratory ware of 167 mm lengths 15796 Round carrier 83x95 mm for 24x5 ml test tubes 13x75 mm for bucket 13831 Round carrier 83x95 mm for 16x10 ml test tubes 17x100 mm for bucket 13831 Round carrier 83x95 mm for 12x15 ml test tubes 17x120 mm for bucket 13831 Round carrier 83x95 mm for 8x30 ml test tubes 26x102 mm for bucket 13831 Round carrier 83x95 mm for 5x50 ml test tubes 30x120 mm for bucket 13831 Round carrier 83x95 mm for 2x100 ml test tubes 45x103 mm for bucket 13831 Round carrier 83x95 mm for 250 ml bottle 62x135 mm for bucket 13831 Round carrier 83x95 mm for 26x2 0 ml test tubes 11x43 mm for bucket 13831 Round carrier 84 mm for 18x5 ml test tubes 13 5x75 mm for bucket 13833 Round carrier 84 mm for 12x10 ml test tubes 17x100 mm for bucket 13833 Round carrier b 84 mm for 12x15 ml test tubes 17x120 mm for bucket 13833 Round carrier 84 mm for 5x30 ml test tubes 26x102 mm for bucket 13833 Round carrier 84 mm for 4x50 ml test tubes 30x120 mm for bucket 13833 Round carrier 84 mm for 100 ml test tubes b 45x103 mm for bucket 13833 Round carrier 84 mm for 250 ml bottle b 62x135 mm for bucket 13833 Round carrier y 84 mm for 24x2 0 ml test tubes 11x43 mm for bu
54. mm for rotor 11761 11762 11763 14151 Round carrier 061 for 1x100 ml test tubes b 46x100 mm for bucket 13174 13178 14152 Round carrier 061 for 1x50 ml Falcon test tubes b 30x120 mm for bucket 13174 13178 14153 Round carrier 61 for 5x15 ml Falcon test tubes b 17 22x120 mm for bucket 13174 13178 14154 Round carrier 61 for 9x 5 ml test tubes 13 5 17x81 mm for bucket 13174 13178 14155 Round carrier 61 for 12x7 5 ml test tubes 4 13 14 5x100 mm for bucket 13174 13178 14156 Round carrier 61 for 8x14 10 ml test tubes 17 17 7x113 mm for bucket 13174 13178 14157 14158 14159 14160 14188 14248 14793 14794 14795 14836 14837 14838 14839 14840 14841 14842 14844 14845 14846 14847 14848 14849 14850 14851 14853 14854 14855 14856 14859 14860 14862 14864 TEST TUBES Catalog no 15011 15016 15017 15015 15040 15046 15048 15050 15051 15052 15053 15054 15055 15056 15067 15102 15115 Round carrier b61 for 4x15 ml test tubes 17 22x120 mm for bucket 13174 13178 Round carrier 061 for 12x2 0 ml Eppendorf test tubes 11x43 mm for bucket 13174 13178 Round carrier 045 for 1x50 ml test tubes 35 5x100 mm for bucket 13833 and round carrier 14850 and buckets 13174 13178 and round carrier 14151 Round carrier 61 for 3x30 25 ml test tubes 25 5x100 mm for bucket 13174 13178 Pad for 100 50 ml test tubes for round carrier 14841 Round carrier 29 8 for 30 25 ml test tubes b 26x100 m
55. n resistor 5 overheating of the motor 6 cover open during centrifuging 7 pressure control actuation 8 the speed sensor is out of operation impulses are missing 9 speed during identification of the rotor other than 85 rpm 10 error of the transmission of the connection 2C 11 rotor unbalanced 12 error of inverter driver communication 13 overheating of the centrifuge above 50 C 14 unused 15 unused With errors of temperature sensors 0 1 2 numbers the code of the resultant error is tied additionally C_TEMP 0 without error C_TEMP 170 the DS18B20 sensor didn t answer 5 orders 1 WRESET C_TEMP 171 incorrect checksum of data received from the sensor With errors referring to the inverter 3 and 12 numbers the code of the error is additionally connected FAL 0 without error FAL 1 too low voltage of the intermediating circuit FAL 2 too high voltage of the intermediating circuit FAL 4 too high temperature of the radiator of the inverter FAL 5 exceeding of the temperature of the motor FAL 9 current overload 1 FAL 10 current overload 2 FAL 13 short circuit FAL 16 the work permit is missing FREIGABE FAL 17 TIME OUT of the RS485 connector FAL 18 start with the incorrect direction FAL 19 exceeding of the speed overdrive 37 FAL 128 FAL FAL FAL FAL FAL FAL FAL FAL FAL FAL FAL 20 129 130 131 150 151 152 252 253 254 255 overflow of the speed meter
56. not allowed to centrifuge explosive and inflammable materials It is not allowed to centrifuge substances prone to reacting in result of supplying high energy during centrifugation Prior to switching the centrifuge on one shall read carefully all sections of this instruction in order to ensure smooth operation and avoid damages of this device or its accessories Centrifuge shall not be operated by unqualified personnel Centrifuge must not be transported with the rotor mounted on the shaft One must use original rotors test tubes and spare parts only In the case of faulty operation of the centrifuge one shall ask for assistance of service of MPW Med instruments Company or its authorized representatives It is not allowed to switch the centrifuge on if it is not installed properly or rotor is not fitted correctly The centrifuge must not be operated in places where explosion hazard exists as it is not explosion proof It is not allowed to centrifuge materials capable of generating inflammable or explosive mixtures when subjected to air It is not allowed to subject to centrifugation toxic or infectious materials with damaged leak proof seals of the rotor or test tube Proper disinfection procedures have to be carried out when dangerous substances contaminated the centrifuge or its accessories 13 A e It isn t allowed to open the cover manually in emergency procedure when rotor is still turning e It isn t allowed to
57. nsistent with requirements of the present day user 4 2 Service elements 1 Cover ee 2 Inspection glass 3 Control panel 4 Emergency cover open place 5 Power switch Drawing 1 General view MPW 380R 1 Motor axle 2 Rotor 3 Rotor cover 1 RS 232 socket 4 Complete clamp 2 Plug in socket Drawing 3 Unit elements of the angle rotor Drawing 2 Back of the centrifuge 11 5 Safe working conditions 5 1 Servicing personnel The MPW 380 R laboratory centrifuge can be operated by laboratory personnel after getting acquainted with User manual User manual shall be held all the time near the centrifuge User manual must be kept always at hand 5 2 Guarantee and operational use period Guarantee period amounts to minimum 24 months Principles are specified in guarantee certificate The service life of the centrifuge specified by the manufacturer amounts to 10 years A After termination of guarantee period it is necessary to carry out yearly technical inspections of the centrifuge Only service personnel authorized by manufacturer may perform the inspections The manufacturer reserves the right to make modifications to produced goods 5 3 Safekeeping period Maximum period of storage of not used centrifuge amounts to 1 year After this period a service authorized by manufacturer should carry out technical inspection of the centrifuge 5 4 Hints on centrifuging Set the ce
58. ntrifuge in horizontal position on rigid base Ensure safe positioning location Ensure free space around the centrifuge amounting to at least 30 cm left free Ensure sufficient ventilation Fix the rotor on the motor axis firmly A e Avoid unbalance e Load opposite buckets with the same accessories e Centrifugation of the test tubes of different sizes There is a possibility to centrifuge test tubes of different sizes however it is absolutely necessary in such cases that opposite buckets and round carriers be the same e Not only the test tubes shall be inserted symmetrically but also round carriers and their hangers shall be equally loaded It is e g not allowed to operate centrifuge only external part of reductive insert loaded 2 A A AN 5 5 Hazards and precautions A It is necessary to insert test tubes symmetrically on the opposite sides Fill test tubes outside the centrifuge Please pay special attention to the quality and proper thickness of the glass test tubes walls Those shall be test tubes for centrifuges of proper durability up to 5 000 x g In order to protect the centrifuge against unbalance fill in the test tubes up to the same weight Lubricate the swing out rotor journal pins Use only accessories in good condition Protect equipment against corrosion using accurate preventive maintenance Infectious materials could be processed in closed buckets only It is
59. o use protective gloves during following work 8 1 Cleaning of the centrifuge For cleaning water with soap or other water soluble mild detergent shall be used One should avoid corrosive and aggressive substances It is prohibited to use alkaline solutions inflammable solvents or agents containing abrasive particles Using wiping cloth remove condensate or residues of the products from the rotor chamber It is recommended to keep the cover opened when the centrifuge does not work in order to expel the moisture In the case the user decides to use centrifuge and equipment cleaning methods other than the ones described in this manual the user shall contact the device manufacturer in order to check whether the cleaning method chosen does not damage the device 8 2 Cleaning of the accessories In order to ensure safe operation one shall carry out in regular way periodical maintenance of the accessories Manufactured rotors buckets and round carriers have to withstand steady high stresses originating from the field of gravitation Chemical reactions as well as corrosion combination of variable pressure and chemical reactions can cause corrosion or destruction of metals Hard to observe surface cracks increase gradually and weaken material without visible symptoms In the case of observation of surface damage crevice or other change as well as the corrosion the given part rotor bucket etc shall be immediately replaced In order to prevent
60. oaded no access to SPEED RCF TIME TEMP ROTORS LIST PARA e A program with modified acceleration or deceleration characteristics loaded ACEL gt 9 DECEL gt 9 no access to SPEED RCF ROTORS LIST e Access password is set No access to CONFIG required providing the password e COOLING active function No access to TEMP 2 CENTRIFUGE IS WORKING e No access to PROGRAM LIST ROTORS LIST and CONFIG and besides e if program No 1 is loaded no access to any zone e if a program with modified acceleration or deceleration characteristics is loaded ACEL gt 9 DECEL gt 9 no access to SPEED and RCF 49 7 9 Unbalance The centrifuge is provided with the rotor unbalance sensor and when it will be activated centrifugation process will be stopped through fast braking and at the same time an error message will be displayed see p 7 5 1 Cancellation of this error is possible only through pressing COVER key after stopping of the rotor One must check if rotor was correctly loaded close the cover and once more start the program In order to protect the rotor against beating in opposite areas of the rotor it has to be provided with identically filled buckets carriers test tubes etc for getting the best balance possible Unbalance causes noise and vibrations during operation and adversely affects power transmission system motor shock absorbers The better balance the smoother will be the centrifuge operation and therefore l
61. of making use 150 1100 11 of the nomogram 120 1000 100 900 10 800 60 A 14 4 cm 9 B 4600 r p m 90 a C 3400 x g 40 6 30 25 8 20 500 15 RCF 400 j n 1000 _ _ m 11 18 r 8 6 r RCF 6 300 Po RN 5 n 4 11 18 1000 3 9 200 Drawing No 5 Nomograph 58 14 2 Table of chemical resistance to the interaction of various categories of reagents of plastics Groups of the substance PS SAN PMMA PC PCV POM PE LD PE HD PP PMP ECTFE PTFE FKM EPDM NR SI in temp 20 C DIE EN Aldehydes 4 z O O 5 O O O O Cyclic alcohols Esters a O O O O Ether O O Ketones z a O O O O O O Strong or concentrated acids Weak or diluted acids O O O O O O Oxidizing acids or oxidizing substances 3 i z gt cyclic hydrocarbons 3 O O O 4 O Ahs O O O Haloid hydrocarbons O O O Alkalis O O very good chemical resistance Permanent action of the substance does not cause damage through 30 days The material 1s able to be resistant through years o chemical resistance of good to limited Continuous action of the substance causes insignificant and partly reversible damage through the period of 7 30 days e g puffing up softening reduced mechanical durability discolouring limited chemical resistance The material should not have the continuous contact with the
62. onger life of usage of the driveline Moreover the ideal separation level is then obtained as already separated constituents would not be moved up by vibration 7 10 Emergency stop In any moment of centrifuging it is possible interrupt the process and fast stop the rotor Single time pressing of the STOP key will make centrifuging stop with acceleration characteristics set in the program Pressing and holding it up to Is will make the centrifuging stop with the most strict characteristic 7 11 Completion of the centrifuging After expiry of the time of centrifuging set in the program the rotor decelerates in accordance with chosen characteristics At the end of deceleration rotational speed drops slower in order to ensure soft settling of rotor carriers After stopping sound signal is generated and the STOP diode goes off After pressing COVER key the cover opens and the COVER diode goes on 7 12 Mathematical relations 7 12 1 RCF relative centripetal force RCF acceleration is the acceleration generated by the rotary motion of the rotor acting upon tested product and it can be calculated according to the formula RCF 11 18 x r x n 1000 RCF xg r cm n rpm Depending on the distance of particles of the tested product from the axis of rotation one can establish with use of the above formula the minimum RCF average RCF or maximum RCF On the basis of preset RCF value and given radius of the bottom of the bucket one can
63. or execute the operation of deleting see p 7 6 9 As opposed to standard user programs program no 1 will not be deleted from the list of programs but 1t will be only removed from the memory of the driver of centrifuge and default settings will be loaded There is a possibility to save the adjusted program by the same number virtually it means the necessity to draw the attention to the number of the program being saved because there is a possibility of accidentally overwriting saved values with new ones Operation of deletion of the program is being realized only in the case of numbers with programs saved If no key will be pressed within 20 seconds the device will automatically return to the main screen 2 7 4 8 Changing acceleration deceleration characteristics numbers ACC DEC 10 19 Loading a modified program in the CURVES fold is signalled by the icon on the main screen Icon that signals loading of the program in this case no 5 in which at least one of characteristics acceleration deceleration parameters was modified by the user it has a number from 10 19 reed IP pee ES EME ENE FROG 3 P Te Since curves created by the user are connected with the number of the rotor and the value of speed set loading of a program modified in such a way 1s subject to certain limitations There is no possibility to change set speed and the rotor number even when the centrifuge is not working It is possible to modify the numbe
64. properly in order to avoid overflows CAUTION Centrifuge will tolerate small weight differences occurring during loading of rotors However it is recommended to equalize vessels loads as much as possible in order to ensure minimal vibrations during operation When the centrifuge is started with large imbalance the unbalance control system will switch off the drive system and error signal will be transmitted On the monitoring panel error message will be displayed see p 7 7 1 In order to prolong lifetime of the rotor and gaskets rotors shall be lubricated with the maintenance oil while gaskets and threaded parts shall be lubricated with the technical petroleum jelly For replacement of the rotor one shall unscrew clamping and then grab the rotor with both hands at opposite sides taking it away from drive shaft by pulling it up 14 6 2 Construction and safety measures The centrifuge has rigid self supporting structure Housing was made of sheet aluminium back made of steel sheet Front and cover was made of ABS type plastic Cover is fixed on steel axles of hinges and from the front it is locked with two electromagnetic locks blocking possible opening during centrifugation Rotation chamber casing was made of thick steel sheet The rotation chamber bowl is made of stainless steel sheet Rotors and containers are from aluminium lids from polycarbonate and reductive inserts from the polypropylene 6 3 Drive Low noise induction motor con
65. r 30 36x15 ml Falcon 5000 3996 143 6 0 11785 Angle rotor 30 48x15 ml Falcon 4700 4025 16 3 8 0 HS hermetically sealed HS accessories maintenance Make sure that rubber O rings are lightly coated with silicone grease Use high vacuum grease e g type C by LUBRINA ZA SWING OUT ROTORS Max Fmax Fmin Cat No Type of rotor Rotor capacity A RCF cm cm 12768 Swing out drum 6 adapters 20 x 0 4 0 2 ml 14500 16450 7 0 3 0 rotor or 10 x 2 0 1 5ml 12786 Swing out rotor 4 x 250 ml 5000 4724 16 9 12 4 12787 Swing out rotor 2x 3 micro titer plates and blocks 4300 2398 116 5 5 12788 Swing out rotor 4 x 4 micro titer plates and blocks 4000 2826 15 8 10 0 12791 Swing out rotor 4 x ASTM 2500 1292 185 40 12830 Swing out rotor 4x400ml 48x15ml 48x15ml 4500 4188 185 8 5 Falcon 8x100 ml 4000 rpm RCF 3309 for MPW 380 BUCKETS Catalog no Application 13080 Bucket q 17 7x87 mm for15 10 7 6 ml test tubes for rotor 11784 11785 13081 Bucket b 17 7x65 for 10 6 5 ml test tubes for rotor 11784 11785 13174 Round bucket 62x107 mm for 250 ml bottles for rotor 12786 13178c Round bucket 62x107 mm for 250 ml bottles with cap 17179 for rotor 12786 13180 Bucket b 30x99 mm for 2 x 50 ml Falcon test tubes for rotor 12786 13275c Bucket b 30x99 mm for 50 30 25 ml Falcon test tubes with cap 17151 for rotor 11780 13276 Bucket b 30x96 mm for 50 30 25 ml Falcon test tubes without thread for rotor 11780 13580 Bucket 045x116 mm
66. r of characteristics connected with the program in the PARA fold setting ACEL and DECEL within the range 0 9 switches centrifuging process to default characteristics and the limitations are no longer applicable 7 4 9 Rotor Bucket It is possible to select the number of the rotor and the number of a relevant container directly on the main screen In order to do so e choose the ROTOR BUCKET NUMBER zone press the SET key choose the zone with UP or DOWN rotor number bucket number will begin to blink press the SET key and choose the right rotor with UP DOWN keys e save the choice through pressing the SET key again 7 4 10 List of rotors It is possible to select the rotor and the container in a more comfortable manner namely from the list The list of rotors contains all data connected with particular rotor In order to browse the list one should e choose the LIST OF ROTORS zone press the SET key choose zone with UP or DOWN keys marker of zone will begin to blink SPEEN E TIME EME Blinking marker of LIST OF ROTORS 26 e press SET the screen with the list of available rotors will be displayed Marker of the rotor used currentl Rotor radius Number of the container maximum Rmax A connected with a given rotor and minimal Rmin SN RCF of rotor e with UP and DOWN keys put the indication marker by the proper number of the rotor e confirm the choice of the rotor with the SET key the device will return
67. ram the next section number 2 The maximum speed of the next section of deceleration characteristic is equal to the speed programmed already of the previous section in the above mentioned case the speed programmed of section 1 3470 rpm highest speed possible for programming of the section 2 3470 rpm New section programming possible the whole line 2 is blinking Programming as in the case of the section 1 To stop creating the deceleration curve at the stage of two sections it is necessary to set the speed in section 2 to 0 and press the SET ke After programming the section 2 there is a possibility to program the next section number 3 New section programming possible the whole line 3 is blinking Programming as in the case of the section 1 To stop creating the deceleration curve at the stage of three sections it is necessary to set the speed in section 3 to O and press the SET ke 43 After programming the section 3 there 1s a possibility to program the next section number 4 Course programmed of acceleration characteristic put together from four sections of starting looks like this New section programming possible the whole line 4 is blinking Programming as in the case of the section 1 If speed of the last section 0 it is possible to save the created characteristics by choosing the EXIT option with UP DOWN keys and pressing the SET ke Repeated attempt to program already programmed sections of the acceleration
68. re referring to the above named temperatures At the change of the place from cold to warm one condensation of water will occur inside the centrifuge It is important then that sufficient time be provided for drying the centrifuge prior to starting the centrifuge again minimum 4 hours 3 3 Connection to mains Supply voltage given on the rating plate has to be consistent with local supply voltage MPW Med ZN instruments laboratory centrifuges are 1 safety class devices and they are provided with the three core cable with the plug resistant to dynamic loadings Mains socket shall be provided with the safety pin It is recommended to install emergency cut out that shall be located far from the centrifuge near the exit or beyond the room Supply voltage 230 V 50 Hz optionally 230 V 60 Hz 115 V 60 Hz A Before switching on check whether the centrifuge is connected to power supply correctly Check centrifuge before usage whether she is installed correctly 3 4 Current protection The centrifuge has thermal current protection of 14 A values 230V TA 45 A124 L140U3 optionally 16A 115V TA 45 A124 L160U4 is situated in the power switch on the left side of the centrifuge 10 4 Description of the centrifuge 4 1 General description New generation of MPW Med instruments laboratory centrifuges is provided with state of the art microprocessor control systems very durable and quiet asynchronous brushless motors and accessories co
69. s The user has a possibility to program up to 10 characteristics of acceleration or deceleration of numbers from 10 to 19 automatic numbering Characteristics shaping process comprises 2 stages e first it is necessary to save current settings in the user s program see p 7 4 6 e then it is necessary to proceed to the CONFIG CURVES fold and load the program saved previously edition of acceleration ACC or deceleration DEC characteristics After choosing the program we want to edit and pressing the SET key the selection window will appear exemplary edition of PROGRAM 3 Number of the program to be edited Modification of acceleration characteristic Return to the CONFIGURATION fol Modification of deceleration Select other program for edition characteristic The message is blinking on the active field With UP and DOWN keys one should select the desired option and confirm it by pressing the SET key Modification of acceleration characteristics Acel After choosing this option the window of the characteristics wizard is displayed Current acceleration characteristics connected with the loaded program will be displayed on the screen Number of section of the Ra 4 2 o Centrifuging speed rpm for the given section of characteristics acceleration characteristics Time in sec after which the centrifuging speed will reach value SPEED Set value target of centrifuging speed Return to the CONFIG fold Total acceleration
70. still pressed for another 3 seconds the speed of repetition is growing three times New value of TIME setup e g 4min 12sec SEEN pe SL da seen were 24044 a rr A a O A A FF Ome AS jo IA ma O cuate seus 5 tro sae wrens O 4 ates as i TE a saree wrrerrerse PELE 1577222355220222372520727227227171231237222272225322772929277205802339307200309220723372025390032022002022722000007099020072520200733200032 200053222325 t aczrm ase a z 7 JM Ba s 4 i 3 a s s a w z Basa gt U oe B a Y U z s U se ASA 4 z e p 4 zaa m a a sea seee U e pressing the SET key ends setting the new value of the centrifuge time New value of the centrifuge time is entered to the time meter REED 7 4 Description of zones 7 4 1 Speed Rotational speed of the rotor Set from 100 rpm speed of identification of rotors is 85 rpm to the maximum admissible rotor speed The settings are changed with UP and DOWN keys in 10 rpm interval Pressing and holding a key longer than one second switches the repetition on If the key is still pressed for another 3 seconds the speed of repetition grows ten times to 100 rpm 7 4 2 Ref The RCF calculation function is highly non linear hence setting the RCF takes place stepwise as per every 10 rpm 21 7 4 3 Time Setting the centrifuging time is divided into 3
71. stitutes the drive 6 4 Data input and output Data setting and read out system forms hermetically closed keyboard with distinctly accessible operation points Easily readable displays signalling individual performed operations facilitate operator s programming and recording of parameters and condition of the centrifuge The centrifuge is provided with the RS 232 serial interface that enables connection of the centrifuge to external PC unit with the printer and recording the centrifugation parameters 6 5 Controls The microprocessor control unit of the centrifuge ensures broad possibilities of providing realisation and reading of work parameters that is gt selection of the spin program from the list 1 99 looking through of programs gt selection of the rotor and containers tied with him according to the accession number from the list looking through of rotors and their parameters gt setting the rotational speed within 100 18 000 rpm at 10 rpm interval gt setting the RCF with automatic calculation of the rotation speed with 10 rpm accuracy gt setting centrifugation time 5 sec 9 min 59 sec resolution 1 sec 10 min 9 h 59 min resolution min or continuous running HOLD gt selection of SHORT short duration operation gt Stand By function display unit blanking after 10 seconds of idle gt counting the time of the START key pressing or of the set speed reached gt displaying th
72. substance The immediate occurrence of damage is possible e g the loss of mechanical durability deformation discolouring bursting dissolving Abbreviations of names of characterized plastics PS Polystyrene ECTFE Ethylene chlorotrifluoroethylene SAN Styrene acrylonitrile ETFE Ethylene tetrafluoroethylene PMMA Polymethyl methacrylate PTFE Polytetrafluoroethylene PC Polycarbonate FEP Tetrafluoroethylene perfluoropropylene PVC Polyvinyl chloride PFA Tetrafluoroethylene perfluoroalkylvinylether POM Acetal polyoxymethylenel FKM Fluorcarbon rubber PE LD Low density polyethylene EPDM Ethylene propylene diene PE HD High density polyethylene NR Natural rubber PP Polypropylene SI Silicon rubber PMP Polymethylpentene 59 14 3 Printing centrifuging USB RS232 PRINTER When the centrifuging process is finished there is a possibility to obtain report Report can be transferred to PC or printed with a AAWKA printer Devices that offer printing reports are available optionally and they are not delivered with every centrifuge PC USB quantity pcs name Connect centrifuge to the PC with the RS232 cable and USB converter connection diagram is given below Install USB converter drivers see attached CD Ensure that serial port COM settings are set as described below 9600 8 n 1 Baud rate 9600 Parity none Stop bit 1 Data length 8 Install MPW Editor application Windows deliver
73. the adjusted RCF value on the main screen thus reducing the radius below Rmax is signalled with the icon in the RCF section of the main screen Icon signalling setting the rotor radius lower than Rmax TAKIE anr Reducing of the rotor radius and the resulting change of displayed RCF value applies until switching off the power supply of the centrifuge or setting the Rmax maximum radius once again loading the program does not change this setting 7 5 4 Densi sample density Standard density of the sample is 1 2 g cm It is possible to set another density within the range from 1 2 for 9 9 g cm3 Increasing this setting above 1 2 g cm limits maximum set centrifuging speed At the same time a relevant marker is displayed on the main screen Icon signaling increasing the density of the sample above 1 2 g cm SHEEN AEP Ie BET PROG 5 2l11766 Increasing density of the sample above 1 2 g cm and limiting of the maximum speed of centrifuging resulting from it applies until switching off power supply of the centrifuge or setting the device back to 1 2 g cm 29 7 5 5 Cooling refrigerating of the centrifuge chamber Checking on this function will cause switching to the TEMP cooling mode in which it is possible to set the cooling temperature Set the temperature with UP and DOWN keys within the range from 0 to ambient temperature SET key confirms the setting The temperature set by COOLING function rang
74. the manufacturer repair 1 Device A sGalNO Aa ee RE NE e Oe 2 Description of decontamination see user Manual 3 Decontamination carried out by naiMe qj AA RA AAA O 4 Date and signature atacando DECLARATION OF DECONTAMINATION In order to protect our employees please fill out the declaration of decontamination completely before sending centrifuge to the manufacturer return 5 Device STYPE O inne A AART serial No 6 Description of decontamination see user manual 7 Decontamination carried out by name 8 Dateandsignature Sidi
75. unbalance Glass particles left in the rotor chamber cause metal abrasion because of strong air circulation This dust will not only contaminate the centrifuge chamber rotor buckets carriers and centrifuged material but will cause as well damages of surfaces of the accessories rotors and the rotation chamber For complete removal of glass particles and metal dust from the rotor chamber it is recommended to place strip of vaseline on the bowl from the top down to bottom Then rotor shall operate for several minutes at moderate speed Glass and metal particles will gather on lubricated area and could be easily removed with the piece of cloth together with the grease This operation can be repeated in case of a need 52 8 5 Sterilization and disinfections of the rotating chamber and accessories One can use all standard disinfectants The centrifuges and accessories are constructed from various materials and one should to take into account possible variety of materials During sterilization by means of steam one should to consider temperature resistance of individual materials STERILIZATION Sterilization Radiation B y Gas Chemical temp 121 C 25 kGy ethylene oxide compounds formalin ethanol FEP PFA yes FKM yes EPDM yes yes Laboratory vessels have to be exactly cleaned and rinsed with the distilled water before the sterilization in the autoclave It is always necessary to remove closures from containers 1 The frequ

MPW-380/R - MPW MED. INSTRUMENTS Spółdzielnia Pracy

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MPW-380/R - MPW MED. INSTRUMENTS Sp&oacute;łdzielnia Pracy

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MPW-380/R - MPW MED. INSTRUMENTS Spółdzielnia Pracy