Agilent 1200 Agilent 1200 Series Valves User Manual
Contents
1. CAN port IJ U J Figure1 Back Panel of the Agilent 1200 Series Valves 12 Agilent 1200 Series Valves Installation and Configuration 1 Hardware Installation Setting up the CAN connection Connect the CAN Cable PN 5181 1519 to one of the CAN ports at back panel of the Agilent 1200 Series valve and to a free CAN Port on another Agilent 1200 Series module The second CAN Port can be used to add additional valves or modules to the HPLC system Setting up the power connection to other Agilent 1200 Series LC modules The power for the Agilent 1200 Series valves can be supplied by one of the following Agilent 1200 Series modules e Agilent 1200 Series preparative pump G1361A e Agilent 1200 Series fraction collectors G1364A G1364B G1364C G1364D e Agilent 1200 Series high performance autosampler G1367B e Agilent 1200 Series micro well plate autosampler G1377A e Agilent 1200 Series dual loop autosampler PS G2258A Connect the DC CAN Cable PN 5181 1533 to the DC CAN connector at the back panel of the valve and to the DC CAN connector on one of the Agilent 1200 Series modules listed above Setting up the power connection to the external power supply If none of the Agilent 1200 Series modules from the list above is part of your HPLC system you have to supply the power through an external power supply P N 0950 4422 Plug the2. Analysis2 Regene Figure 19 Alternating column regeneration time scheme We recommend to use the Agilent Technologies Capillary kits for Alternating Column Regeneration These kits contain all capillaries fittings and ferrules that are required for the setup displayed in Figure 18 on page 40 Agilent Technologies offers one kit for analytical scale applications flow rates lt 10 ml min that contain the capillaries listed in Table 7 on page 42 and fittings listed in Table 8 on page 42 Another kit is suited for preparative scale applications flow rates lt 100 ml min Its capillaries are listed in Table 9 on page 43 and its fitting are listed in Table 10 on page 43 Agilent 1200 Series Valves 41 4 42 Valve Applications Alternating Column Regeneration Capillary Kit G1156 68711 for alternating column regeneration with G1157A analytical scale Table7 Capillaries From To ID mm Length mm part number Injector Autosampler Valve port 2 0 17 500 G1328 87600 Valve port 3 Column 1 0 17 600 5021 1819 Column 1 Valve port 6 0 17 400 5065 9933 Valve port 7 Detector 0 17 600 5065 9933 Valve port 8 Column 2 0 17 400 5021 1819 Column 2 Valve port 1 0 17 600 5065 9933 Valve port 5 Valve port 10 0 17 105 5021 1816 Regeneration Pump Valve port 4 0 25 800 5065 9930 Valve port 9 Waste 0 6 PTFE 2000 0890 1713 Table8 Fittings screws and ferrules Description Qty part number 1 16
3. 1 15 1 Installation and Configuration Softw are Configuration 16 Agilent 1200 Series Valves Agilent 1200 Agilent 1200 Series Valves User Manual 2 Operation Operating the Valve using Agilent ChemStation 18 Handheld Controller functions 22 dg Agilent Technologies 17 2 Operation Operating the Valve using Agilent ChemStation Operating the Valve using Agilent ChemStation After the Agilent 1200 Series Valves have been installed and configured the valve parameters can be edited in the Agilent ChemStation Choose Setup Valve from the Instrument menu to open the valve dialog box as displayed in Figure 3 Setup Valve lt 2P5 10PT gt Instrument Figure3 Valve Setup dialog box The example displays the valve setup for the 2 position 10 port valve G1157A The interface for the other valves are similar Position When loading a method the valve is switched to the position that is displayed in the Position dropdown box If you select Use Current the valve remains in the current position when a new method is loaded If you select a new position in the dropdown box and click OK the Setup Valve dialog box will be closed and the valve switches to the new position 18 Agilent 1200 Series Valves Operation 2 Operating the Valve using Agilent ChemStation After the run is finished the valve always switches to the starting position If Use current is selected the valve remains in the current posi
4. Agilent 1200 Series Valves 43 4 Valve Applications Sample enrichment and Sample stripping Sample enrichment and Sample stripping Sample enrichment and sample stripping methods can be used to separate samples from complex matrices such as biological fluids food extracts or wastewater This might be necessary to avoid interference of the matrix during separation and detection or even damage of the column You can use the Agilent 1200 Series 2 position 6 port valve G1158A to automate these techniques for LC and LC MS analysis Sample enrichment Sample enrichment is used to increase the sensitivity and to remove the sample matrix The technique is displayed in Figure 20 on page 44 This technique allows the injection of large volumes onto the precolumn where the sample matrix is separated from the analytes in order to increase sensitivity PumpA Autosampler Analytical column Position 1 nedi Detector Enrichment Pump B column Pump A Autosampler gx Waste Position 2 Enrichment Pump B column Figure 20 Sample enrichment schematic setup 44 Agilent 1200 Series Valves Valve Applications 4 Sample enrichment and Sample stripping For the sample enrichment phase the valve is switched to position 1 The eluent Pump transfers the injected sample onto the enrichment column The sample is retained and enriched on this column whereas the sample matrix is flushed into the waste At the same time the second eluent p
5. Agilent 1200 Series Valves Agilent 1200 Agilent 1200 Series Valves User Manual 7 Safety Information General Safety Information 66 Lithium Batteries Information 69 Radio Interference 70 SoundEmission 71 Solvent Information 72 Agilent Technologies on Internet 73S dg Agilent Technologies 65 7 Safety Information General Safety Information 66 The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Aligent Technologies assumes no liability for the customer s failure to comply with these requirements General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever
6. Solvent Information 72 Observe the following recommendations on the use of solvents Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl3 Og 3 2COClg 2HCU This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Agilent 1200 Series Valv
7. fittings and ferrules 10 pk 1 5062 2418 fingertight fitting long 1 5062 8541 10 pk fitting screw long 10 5065 4454 10 pk fitting screw extra long 4 5065 9967 10 pk front ferrule 10 pk 1 5180 4108 back ferrule 10 pk 1 5180 4114 ZDV fitting 1 0100 0900 0 18 ID PEEK tubing 1 5 m 1 0890 1763 Plastic tubing cutter 1 8710 1930 Agilent 1200 Series Valves Valve Applications 4 Alternating Column Regeneration Capillary Kit G1156 68713 for alternating column regeneration with G1157A preparative scale Table9 Capillaries From To ID mm Length mm part number Injector Autosampler Valve port 2 0 5 600 G2260 87300 Valve port 3 Column 1 0 5 600 62260 87301 Column 1 Valve port 6 0 5 400 G2260 87300 Valve port 7 Detector 0 5 600 62260 87300 Valve port 8 Column 2 0 5 400 G2260 87301 Column 2 Valve port 1 0 5 600 62260 87300 Valve port 5 Valve port 10 0 5 105 5065 9927 Regeneration Pump Valve port 4 0 5 800 5065 9926 Valve port 9 Waste 0 6 PTFE 2000 0890 1713 Table 10 Fittings screws and ferrules Description Qty part number 1 16 fittings and ferrules 10 pk 1 5062 2418 fingertight fitting long 1 5062 8541 10 pk fitting screw long 10 5065 4454 10 pk fitting screw extra long 4 5065 9967 10 pk front ferrule 10 pk 1 5180 4108 back ferrule 10 pk 1 5180 4114 Union Prep 1 5022 2133 0 5 ID PEEK tubing 1 5 m 1 0890 1761 Plastic tubing cutter 1 8710 1930
8. 0101 1363 valve Agilent 1200 Series Valves 55 5 Parts Capillaries and Fittings for analytical and preparative flow rates Table 20 Capillary and tubing kits Description part number Capillary kit alternating column regeneration analytical for valve G1157A for details see Table 7 and Table 8 on page 42 Capi lary kit alternating column regeneration preparative for valve G1157A for details see Table 9 and Table 10 on page 43 Capi ana lary kit alternating Sample enrichment stripping ytical for valve G1158A for details see Table 11 and Table 12 on page 47 Capi with lary kit column selection analytical for 6 columns valve G1159A for details see Table 13 and Table 14 on page 49 Tubing kit solvent selection for 4 solvents lt 10 ml min for valve G1160A for details see Table 15 and Table 16 on page 51 G1156 68711 G1156 68713 G1156 68714 G1156 68712 G1156 68706 Table 21 Part Numbers for flexible SST capillaries without fittings volume in parentheses length in mm 0 12 mm ID 0 17 mm ID 0 25 mm ID 0 50 mm ID 105 5021 1820 1 2ul 5021 1816 2 4l 5065 9927 20 6 150 5021 1821 1 7 5021 1817 3 4 5022 6509 29 5ul 200 5065 9931 4 6ul 280 5021 1822 3 2ul 5021 1818 6 41 5062 6508 13 84 5022 6510 55 0ul 400 5021 1823 4 541 5021 1819 9 11 G2260 87301 78 5ul 500 G1328 87600 11 4l 600 5065 9933 13 61 G2260 87300 117 8pl 700 5065 9
9. DC CAN adapter from the power supply into the DC CAN connector on the back panel of your Agilent 1200 Series valve Agilent 1200 Series Valves 13 1 Installation and Configuration Softw are Configuration Softw are Configuration The Agilent 1200 Series valves can be controlled by the Agilent ChemStation Rev A 09 03 or higher The maximum number of all modules including CAN slave valves in your Agilent 1200 CAUTION Series LC system must not exceed 13 Contact your local sales and service representative to verify if your configuration is supported This is especially important if the number of module is close to 13 To configure the Agilent 1200 Series valves Step Note 1 Start the Agilent ChemStation Software N Select Configure 1200 Access in the Instrument menu UJ In the upcoming Configuration dialog The selected green valve icon now box select one or multiple valves and moves from the left Available M odules click Add panel to the right Configured M odules panel as displayed in Figure 2 on page 15 4 Click OK to leave the Configuration dialog box ul After a Restart the Agilent ChemStation Software a new menu item Setup Valve now appears in the Instrument menu 14 Agilent 1200 Series Valves Software Configuration Figure 2 Agilent 1200 Series Valves Installation and Configuration LPOOUDDKEE Gaaa DE eee okt ny B G1311A 2 Quatemay DETT e042 Valve configuration
10. It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met Agilent 1200 Series Valves In This Guide Agilent 1200 Series Valves The Agilent 1200 Series valves provide the user a comprehensive solutions for more flexibility through solvent selection and column selection They offer new automation capabilities for sample preparation as well as higher sample throughput with alternating column regeneration The Agilent 1200 Series valves are fully integrated in the CAN environment control area network of the Agilent 1200 Series LC system and can be controlled by the Agilent ChemStation Software The following types of valves will be described in this manual G1157A Agilent 1200 Series 2 Position 10 Port Valve G1158A Agilent 1200 Series 2 Position 6 Port Valve G1159A Agilent 1200 Series 6 Position Selection Valve G1160A Agilent 1200 Series 12 Position 13 Port Selection Valve G1162A Agilent 1200 Series 2 Position 6 Port Micro Valve G1163A Agilent 1200 Series 2 Position 10 Port Micro Valve These valves and the corresponding capillary kits for alternating column regeneration sample enrichment sample clean up column selection or solvent selection will allow you to implement these applications easily Chapter 1 Installat
11. Please report missing or damaged parts to your Agilent Technologies sales and service office Table3 Delivery checklist for Agilent 1200 Series valves G1157A G1158A G1159A and G1160A Quantity Description Part Number 1 Agilent 1200 Series Valve 1 Declaration of conformity 1 Rail assembly for Column Organizer 1 Accessory kit containing 1 e CAN Cable 1m long 5181 1519 1 DC CAN cable 5181 1533 10 e Fitting Screw long 5065 4454 12 e Fitting Screw extra long 5065 9967 10 pk Agilent 1200 Series Valves 9 1 10 Installation and Configuration Unpacking the Agilent 1200 Series valve Table3 Delivery checklist for Agilent 1200 Series valves G1157A G1158A G1159A and G1160A continued Quantity Description Part Number 1 e Hex Key 9 64 T handle 8710 2394 1 e Hex Key 3 32 1 e wrench 8710 0510 1 socket wrench Rheotool 1 4 8710 2391 Table 4 Delivery checklist for Agilent 1200 Series micro valves G1162A and G1163A Quantity Description Part Number 1 Agilent 1200 Series Micro Valve 1 Declaration of conformity 1 Rail assembly for Column Organizer 5065 4450 6 10 M4 micro valve fittings 5065 4410 1 M4 micro valve plug 5065 4410 1 Accessory kit containing 1 e CAN Cable 1m long 5181 1519 1 DC CAN cable 5181 1533 1 e Hex Key 9 64 T handle 8710 2394 1 e Hex Key 3 32 1 wrench 8710 0510 1 socket wrench Rheotool 1 4 8710 2391 The consumable kit 5065 4410 contains six M 4 micro val
12. Position 13 Port Selection Valve e G1162A Agilent 1200 Series 2 Position 6 Port Micro Valve e G1163A Agilent 1200 Series Agilent Technologies 2006 Printed in Germany 02 06 G1136 80010 G1156 90010 77 Agilent Technologies
13. Series Valves Agilent 1200 Agilent 1200 Series Valves User Manual 4 Valve Applications Alternating Column Regeneration 40 Sample enrichment and Sample stripping 44 Column Selection 48 Solvent Selection 50 In this chapter selected applications for the Agilent 1200 Series Valves will be described This description will contain e short description e flow diagrams part numbers of capillaries fittings and capillary kits 2 s Agilent Technologies 39 4 Valve Applications Alternating Column Regeneration Alternating Column Regeneration Alternating column regeneration is a convenient way to increase the sample throughput The Agilent 1200 Series 2 position 10 port valve G1157A can be used in combination with the Agilent LC and LC MS modules to increase the efficiency in laboratories running large amounts of samples Gradient elution is a common technique for separation of complex samples in liquid chromatography which requires column regeneration before the subsequent run is started Using alternating column regeneration valuable time for the analysis is saved Core of the alternating column regeneration is the Agilent 1200 Series 2 position 10 port valve which allows simultaneous analysis on one column while a second identical column is flushed and equilibrated The schematic setup is displayed in Figure 18 Position 1 gt Column 2 Fluent 10 pump Autosampler 1 G 9 Regeneration pump Positi
14. each of the screws an equal amount until the they are finger tight then tighten them another half turn Agilent 1200 Series Valves 29 3 Maintenance Repair and Troubleshooting Maintenance and Repair Replacing the rotor seal for micro valves G1162A and G1163A Disassembling the valve head 1 Use the Hex Key to remove the Stator Screws 1 from the Stator Head 2 2 Disassemble the Stator Head 2 3 Remove the three Stator Ring Screws 3 and take off the Stator Ring 4 4 Remove the Rotor Seal 5 from the Valve Body 6 The Rotor Seal is mounted on three pins cL EEE O O Figure 11 micro valve head assembly 30 Agilent 1200 Series Valves Maintenance and Repair Maintenance Repair and Troubleshooting 3 Reassembling the valve head 1 Mount the new Rotor Seal 5 with the grooves facing the Stator Head 2 The three pins on the Shaft Assembly fit into the matching holes in the Rotor Seal only one way 2 Mount the Stator Ring on the Shaft Assembly that the two pins fit to the matching holes on the Valve body 3 Assemble the Stator Head 2 that the pin in the Stator Ring fits to the matching hole in the Stator Head 4 Insert the Stator Screws 1 Turn each of the screws an equal amount until the they are finger tight then tighten them another half turn Table6 Rebuild kits and repair parts for Agilent
15. it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuseholders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not Agilent 1200 Series Valves Agilent 1200 Series Valves Safety Information attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous volt
16. line in the Time Table above the selected position Appends a line at the end of the Time Table Cuts the selected line s out of the Time Table and saves it to the clipboard Copies the selected line s from the table to the clipboard Pastes line s from the Clipboard to the Time Table Agilent 1200 Series Valves Operation 2 Operating the Valve using Agilent ChemStation Next position after run If Next position after run is checked the valve will switch to the next available position after the run is completed and remains there for the next run For this selection the Position field is greyed out see Figure 4 on page 19 Display instrument actuals In order to display the current status of your Agilent 1200 Series valve choose Instrument Actuals form the View menu in the Agilent ChemStation to open the Valve Status dialog box Figure 5 Valve 12P5 13PT Status Act Pos 1 Solvent 1 Hame Solvent Selector Figure5 Instrument Actuals Agilent 1200 Series Valves 21 2 Operation Handheld Controller functions Handheld Controller functions 22 With firmware revision B 03 11 or higher the following valve functions with the Agilent 1200 Series control module G1323B are available Complete control of an Agilent 1200 Series valve during an LC analysis requires ChemStation Revision A 09 03 or higher Switching the Valve 1 From the startup screen of the Agilent 1200 Series control module select System F5 2 Se
17. 1200 Series valves Description part number e Rebuilt kits Rebuild kit for G1157 2 position 10 port valve 0101 1359 EE Rebuild kit for G1158 2 position 6 port valve 0101 1358 Rebuild kit for G1159 6 position selection valve 0101 1290 Rebuild kit for G1160 12 position 13 port valve 0101 1288 e Stator heads Rotor seals for G1158 2 position 6 port valve Rotor seal for G1162A 2 positi valve Rotor seal for G1163A 2 positi valve Stator head for G1157 2 positi Stator head for G1158 2 positi Stator head for G1159 6 positi Stator head for G1160 12 posi Stator head for G1162A 2 posi valve Stator head for G1163A 2 pos valve on 6 port micro on 10 port micro on 10 port valve on 6 port valve on selection valve tion 13 port valve tion 6 port micro ition 10 port micro 0100 1855 Vespel 0100 1854 Tefzel 0100 2233 PEEK 0100 2087 0101 1361 0101 1362 0100 1850 0101 1364 0101 1365 0100 2089 0101 1363 Agilent 1200 Series Valves 31 3 32 Maintenance Repair and Troubleshooting Maintenance and Repair Early Maintenance Feedback EM F Depending on the application the valve will perform many thousands of actuations without any visible signs of wear In order to minimize downtime the Agilent ChemStation offers Early Maintenance Feedback EMF for the Agilent 1200 Series valves EMF monitors the number of switches of the Agilent 1200 Series val
18. 5 700 3 092 E G1315 68708 50 150 0 295 B C G1375 87300 50 200 0 392 B C G1375 87302 Agilent 1200 Series Valves Parts 5 Table 24 PEEK coated fused silica capillaries for micro valves continued i D um Length mm Volume ul Fitting type Part number 50 220 0 432 B B G1375 87301 50 280 0 550 C D G1375 87309 50 400 0 785 E D G1315 68703 50 500 0 982 C D G1375 87304 50 550 1 080 B C G1375 87310 25 100 0 049 C D G1375 87320 25 220 0 108 D D G1375 87321 25 350 0 172 C D G1375 87322 25 550 0 270 C D G1375 87323 25 700 0 344 C D G1375 87324 seeTable 23 on page 58 Agilent 1200 Series Valves 59 5 Parts 60 Agilent 1200 Series Valves Agilent 1200 Agilent 1200 Series Valves User Manual 6 ee 6 oe Specifications e G1157A Agilent 1200 Series 2 position 10 port valve 62 G1158A Agilent 1200 Series 2 position 6 port valve 62 G1159A Agilent 1200 Series 6 position selection valve 62 G1160A Agilent 1200 Series 12 position 13 port selection valve 63 G1162A Agilent 1200 Series 2 position 6 port micro valve 63 G1163A Agilent 1200 Series 2 position 10 port micro valve 63 In this chapter you will find the specifications of the Agilent 1200 Series valves dg Agilent Technologies 61 6 62 Specifications Table 25 G1157A Agilent 1200 Series 2 position 10 port valve Liquid contacts Stainless Steel and PEEK Port size Accepts 10 32 male threaded fittings Flow passage diameters Stator a
19. 932 15 941 800 5065 9930 39 341 5065 9926 157 56 Agilent 1200 Series Valves Capillaries and Fittings for micro valves Parts 5 For the operation of the Agilent 1200 Series micro valves we recommend the following fittings and PEEK coated fused silica capillaries as listed in Table 22 Table 23 and Table 24 Table 22 Fittings and Ferrules for micro valves Fitting Type Name Swagelok Lite Touch Lite Touch Rheodyne Finger Tight Lite touch Detector E Lite touch Detector mI 0 O WO Ww Description 1 16 SST fitting front and back ferrule 1 16 SST fitting male wrench size 4 1 32 SST ferrule and lock ring M4 PEEK fitting Double winged nuts and 1 32 ferrules 1 16 SST fitting male wrench size 4 SST ferrule Units of measure Part number 10 pk 10 pk 10 pk 6 fitt 2 plug 10 pk 10 pk 10 pk 5062 2418 5063 6593 5065 4423 5065 4410 5065 4422 5063 6593 5063 6592 Agilent 1200 Series Valves 57 Parts Table 23 Fitting Types Fittings and ferrules Fitting type Bai A hm C Table 24 PEEK coated fused silica capillaries for micro valves iDJ um Length mm Volume pl Fitting type Partnumber 100 150 1 178 B C G1375 87317 100 200 1 570 B C G1375 87312 100 220 1 728 B B 61375 87305 100 550 4 320 B C G1375 87306 100 1100 8 639 B D G1375 87315 100 1100 8 639 B B G1375 87303 75 400 1 767 D E G1375 87308 75 500 3 209 CID G1375 87311 7
20. Agilent 1200 Series Valves User Manual oie Agilent Technologies Notices Agilent Technologies Inc 2006 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number G1156 90010 Edition 02 06 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses T
21. Switches 8 Limit EPP Figure8 Early Maintenance Feedback EMF Screen Agilent 1200 Series Valves 23 2 Operation Handheld Controller functions 24 Agilent 1200 Series Valves Agilent 1200 Agilent 1200 Series Valves User Manual 3 Maintenance Repair and Troubleshooting Status Indicators 26 Maintenance and Repair 27 System Errors and Troubleshooting 34 dg Agilent Technologies 25 3 Maintenance Repair and Troubleshooting Status Indicators Status Indicators 26 The instrument status indicator indicates one of four possible instrument conditions When the status indicator is OFF the instrument is in a prerun condition and is ready to begin an analysis A green status indicator indicates the instrument is performing an analysis run mode A yellow status indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed or while a self test procedure is running The next programmed injection will be inhibited until the not ready condition has been cleared An error condition is indicated when the status indicator is red An error condition indicates the instrument has detected an internal problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis and prevents the ne
22. ages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting 7 67 7 Safety Information Safety Symbols Table 31 shows safety symbols used on the instrument and in the manuals Table 31 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to AN protect the apparatus against damage p Indicates dangerous voltages Indicates a protected ground terminal WARNING A warning alerts you to situations that could cause physical injury or damage to the equipment Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could cause a possible loss of data Do not proceed beyond a caution until you have fully understood and met the indicated conditions 68 Agilent 1200 Series Valves Safety Information 7 Lithium Batteries Information Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent type recommended by the equipment manufacturer Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations
23. alidation 48 micro valves 27 multi column setup 48 N notready 34 P position 18 20 position unknown 36 pow fail status 35 power supply external 13 preparative scale alternating column regeneration 43 R rebuild kit 27 55 repair parts 31 55 rotor seal 28 30 S Safety information on lithium batteries 69 sample enrichment 44 analytical scale 46 sample matrix removal 44 sample stripping 45 sample throughput 40 selection valve 48 sensitivity 44 setup valve 18 site requirements 8 solvent selection 50 tubing kit 51 specifications 61 stator face 28 stator head 28 30 75 Index stator ring 28 30 Status Indicators 26 switch failure 35 switching the valve 22 synchronization 22 37 system errors 34 T time table 20 troubleshooting 34 tubing kit 51 56 U unknown position 36 use current 18 uv radiation 72 V valve assemblies 54 valve configuration 15 valve head 28 30 valve name 19 valve parameters 18 valve synchronization 37 76 Agilent 1200 Series Valves www agilent com In This Book This User s Guide describes common applications as well as installation operation and maintenance of the following Agilent 1200 series valves e G1157A Agilent 1200 Series 2 Position 10 Port Valve e G1158A Agilent 1200 Series 2 Position 6 Port Valve e G1159A Agilent 1200 Series 6 Position Selection Valve e G1160A Agilent 1200 Series 12
24. d 1 Use the Hex Key to remove the Stator Screws 1 from the Stator Head 2 2 Disassemble the Stator Head and Stator Face 5 from the Stator Ring 4 The Stator Face usually remains on the Stator Head 3 Remove the three Stator Ring Screws 3 and take off the Stator Ring 4 4 Remove the Rotor Seal 6 from the Valve Body 7 The Rotor Seal is mounted on three pins and can be pulled off TO 5 T Figure9 Valve head assembly Reassembling the valve head 1 Mount the new Rotor Seal 6 with the slots facing the Stator Head 2 The three pins 8 on the Shaft Assembly fit into the matching holes 9 in the Rotor Seal only one way 28 Agilent 1200 Series Valves Maintenance Repair and Troubleshooting 3 Maintenance and Repair Figure 10 Valve body 7 and Rotor seal 6 2 Align the Stator Ring 4 that the two short pins on the ring enter the matching holes in the body 7 3 Insert the three Stator Ring Screws 3 Turn each of the screws an equal amount until the they are finger tight then tighten them another half turn 4 Mount the new Stator Face 5 onto the Stator Head 2 The pins on the Stator face must fit into the matching holes of the Stator Head 5 Assemble the Stator Head 2 and Stator Face 5 that the pin in the Stator Ring enters the matching hole in the Stator Head 6 Insert the Stator Screws 1 Turn
25. e Applications Column Selection Column Selection With the Agilent 1200 Series 6 position selection valve G1159A and the capillary kit for Column Selection Table 13 and Table 14 on page 49 you can set up your Agilent 1200 Series LC or LC MS system for use with up to 6 columns as displayed in Figure 22 Or you can use the system with 5 columns and one flow path for flow injection analysis or for flushing the system This setup allows you to switch between these columns for faster method development or method validation The multi column setup might also be used if several operators are sharing the LC or LC MS system Auto Pump sampler Figure 22 Column selection schematic setup 48 Agilent 1200 Series Valves Column Selection Valve Applications 4 Capillary Kit G1156 68712 for column selection with G1159A analytical scale Table 13 Capillaries From To Qty ID mm Length mm part number Injector Autosampler Valve IN 1 0 17 500 G1328 87600 Valve X ColumnX 6 0 17 400 5021 1819 Column X Valve X 6 0 17 400 5021 1819 Valve OUT Detector 1 0 17 600 G1328 87600 Table 14 Fittings screws and ferrules Description Qty part number 1 16 fittings and ferrules 10 pk 2 5062 2418 fitting screw long 15 5065 4454 10 pk front ferrule 10 pk 1 5180 4108 back ferrule 10 pk 1 5180 4114 ZDV fitting 1 0100 0900 0 18 ID PEEK tubing 1 5 m 1 0890 1763 Plastic tubing cutter 1 8710 1930 Agilent 1200 Se
26. e valve didn t switch correctly Actions 1 Synchronize the valve as described on page 37 2 Restart the ChemStation 3 Disconnect and replug the CAN cable Agilent 1200 Series Valves 35 3 36 Maintenance Repair and Troubleshooting System Errors and Troubleshooting Unknown Position Valve 12PS 13PT Status Act Pos 0 Name LC MSD Figure 16 Unknown Position Error Cause An unknown position is indicated Act Pos 0 Actions 1 Synchronize the valve as described on page 37 2 Restart the ChemStation 3 Disconnect and replug the CAN cable Agilent 1200 Series Valves Maintenance Repair and Troubleshooting 3 System Errors and Troubleshooting Valve Synchronization If the valve failed to switch or if the current position of the valve is unknown it might be necessary to synchronize the valve To synchronize the valve Step Note 1 Switch to the Diagnostics view 2 Select Valve from the Maintenance menu The valve should now display the Current Position as displayed in Figure 17 If the current position is 0 itis unknown 3 To synchronize the valve choose any position from the Synchronize to position dropdown list and click OK Valve Maintenance Synchronize to position Current position Reset Valve Switching Counter Figure 17 Valve Synchronization Agilent 1200 Series Valves 37 3 Maintenance Repair and Troubleshooting System Errors and Troubleshooting 38 Agilent 1200
27. ernal Power Supply 0950 4422 e Rail assembly for Column Organizer 5065 4450 Table 18 Replacement valve assemblies Description part number e 2position 10 port valve G1157 60001 2position 6 port valve G1158 60001 6position selction valve G1159 60001 12 position 13 port selection valve e 2 position 6 port micro valve e 2position 10 port micro valve G1160 60001 G1162 60001 G1163 60001 Agilent 1200 Series Valves Parts 5 Table 19 Rebuild kits and repair parts for Agilent 1200 Series valves Description part number e Rebuilt kits Rebuild kit for G1157A 2 position 10 port valve 0101 1359 and rotor seals Rebuild kit for G1158A 2 position 6 port valve 0101 1358 Rebuild kit for G1159A 6 position selection valve 0101 1290 Rebuild kit for G1160A 12 position 13 port valve 0101 1288 Rotor seals for G1158A 2 position 6 port valve 0100 1855 Vespel 0100 1854 Tefzel 0100 2233 PEEK Rotor seal for G1162A 2 position 6 port micro 0100 2087 valve Rotor seal for G1163A 2 position 10 port micro 0101 1361 valve e Statorheads Stator head for G1157A 2 position 10 port valve 0101 1362 Stator head for G1158A 2 position 6 port valve 0100 1850 Stator head for G1159A 6 position selection valve 0101 1364 Stator head for G1160A 12 position 13 port valve 0101 1365 Stator head for G1162A 2 position 6 port micro 0100 2089 valve Stator head for G1163A 2 position 10 port micro
28. es Safety Information 7 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1200 Series modules for download Agilent 1200 Series Valves 73 7 Safety Information Agilent Technologies on Internet 74 Agilent 1200 Series Valves Index A accessory kit 9 Agilent ChemStation 14 18 Agilent on internet 73 alternating column regeneration 40 analytical scale 42 preparative scale 43 analytical scale alternating column regeneration 42 column selection 49 sample enrichment 46 battery safety information 69 C CAN connection 13 CAN failure 35 capillaries 58 capillary kits 56 column organizer 12 column selection 48 analytical scale 49 configuration software 14 crystal formation 27 D DC CAN Cable 13 delivery checklist 9 diagnostics 32 E early maintenance feedback EMF 23 32 Agilent 1200 Series Valves EMF counter 33 EMF info pad 32 EMF limit 23 32 F ferrules 58 finger tight fittings 51 fittings 51 58 flow injection analysis 48 flow range 62 64 flushing 48 G gradient elution 40 H handheld controller 22 information on uv radiation 72 instrument actuals 21 internet 73 L large volumes 44 M method development 48 method v
29. for batteries Lithiumbatteri Eksplosionsfare ved fejlagtic handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandoren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Agilent 1200 Series Valves 69 7 Safety Information Radio Interference Never use cables other than the ones supplied by Aligent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and M easurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 70 Agilent 1200 Series Valves Safety Information 7 Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A At Operator Position e Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test Agilent 1200 Series Valves 71 7 Safety Information
30. he hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 J une 1995 oras a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 J une 1987 or any equivalent agency regulation or contract clause Use duplication or disclo sure of Software is subject to Agilent Tech nologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 J une 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 J une 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard
31. ion and Configuration starting on page 7 will describe how to install and configure the Agilent 1200 Series valves Chapter 2 Operation starting on page 17 will describe the Agilent ChemStation valve interface and how to setup the valves in your analytical method Chapter 3 Maintenance Repair and Troubleshooting starting on page 25 will describe recommended maintenance and repair procedures as well as troubleshooting tools Chapter 4 Valve Applications starting on page 39 will describe common application e g alternating column regeneration and how to setup your valves for these applications Agilent 1200 Series Valves contents Contents 1 Installation and Configuration Site Requirements 8 Unpacking the Agilent 1200 Series valve 9 Damaged Packaging 9 Delivery Checklist 9 Hardware Installation 12 Setting up the CAN connection 13 Setting up the power connection to other Agilent 1200 Series LC modules 13 Setting up the power connection to the external power supply 13 Software Configuration 14 2 Operation Operating the Valve using Agilent ChemStation 18 Handheld Controller functions 22 3 Maintenance Repair and Troubleshooting Status Indicators 26 Maintenance and Repair 27 Replacing the stator face and the rotor seal for standard valves G1157A G1158A G1159A G1160A 28 Replacing the rotor seal for micro valves G1162A and G1163A 30 Early Maintenance Feedback EMF 32 Sys
32. lect Control F5 and then Valve to open the screen that is displayed in Figure 6 Use the arrow keys up and down to switch the valve to the next position Figure6 Switching the Agilent 1200 Series valve Valve Synchronization 1 From the startup screen of the Agilent 1200 Series control module select System F5 2 Select Tests F3 and Valve to open the Synchronization screen as displayed in Figure 7 3 Press Synchronize F7 and select the position for the valve synchronization Please also read the section Valve Synchronization on page 37 Agilent 1200 Series Valves Operation 2 Handheld Controller functions Figure7 Valve Synchronization Setting the EMF limit and switch counter 1 From the startup screen of the Agilent 1200 Series control module select System F5 2 Select Records F4 and scroll through the displayed module list and select the Agilent 1200 Series Valve 3 Press EMF F1 and then select 1 Setup Limits 4 Inthe upcoming dialog Figure 8 edit the EMF limit If this limiting number of switches has been reached you will get an Early Maintenance Feedback signal on your Handheld controller and in the Graphical User Interface of the Agilent ChemStation Software After you have done an maintenance action press Reset F7 to reset the switch counter Also refer to the section Early Maintenance Feedback EMF on page 32 m 611574 Column Regenration Valve Serial LPOOOOO03 Valve
33. nd stator face assembly 0 6 mm 0 024 rotor seal 0 6 mm 0 024 Volume in flow passage Stator includes stator face seal 2 1 ul hole rotor seal 0 7 ul groove Maximum pressure 41 M Pa 408 bar 6000 psi Recommended flow range 0 2 100 ml min Table 26 G1158A Agilent 1200 Series 2 position 6 port valve Liquid contacts Stainless steel PEEK and alumina ceramic Port size Accepts 10 32 male threaded fittings Flow passage diameters Stator and stator face assembly 0 4 mm 0 015 rotor seal 0 5 mm 0 018 Volume in flow passage Stator includes stator face seal 0 7 ul hole rotor seal 0 3 ul groove Maximum pressure 41 M Pa 408 bar 6000 psi Recommended flow range 0 2 100 ml min Table 27 G1159A Agilent 1200 Series 6 position selection valve Liquid contacts Stainless steel and PEEK Port size Accepts 10 32 male threaded fittings Flow passage diameters Stator 0 6 mm 0 024 stator face assembly and rotor seal 0 4 mm 0 015 Volume in flow passage Angled ports 1 2 5 15 6 ul Radial ports 2 4 6 18 8 ul Maximum pressure 35 M Pa 345 bar 5000 psi Recommended flow range 0 3 40 ml min Agilent 1200 Series Valves Specifications 6 The G1159A Agilent 1200 Series 6 positions selection valve can be used at flow rates up to 100 ml min but without valve switching In most cases e g column selection the valve switches during the postrun or prerun when the flo
34. on 2 Column 2 Elvant pump Autosampler Regeneration gt Detector pump Column 1 Figure 18 Alternating Column Regeneration schematic setup 40 Agilent 1200 Series Valves Valve Applications 4 Alternating Column Regeneration If the valve is switched to position 1 the eluent pump delivers the mobile phase through the injection loop of the autosampler into port 2 of the Agilent 1200 Series Valve The sample is separated on column 1 and analyzed by the detector Simultaneously a second regeneration pump flushes and equilibrates column 2 After the analysis of the sample is finished on column 1 column 2 is prepared for an immediate injection The 2 position 10 port valve is switched and the sample can be injected and analyzed While the analysis is running on column 2 column 1 is now regenerated and will be ready to use immediately after the sample run on column 2 is finished The advantage of this technique is visualized in Figure 19 where the time of analysis with sequential column regeneration is compared to time of analysis using alternating column A detailed description how to setup alternating column regeneration with an Agilent 1200 Series LC system is provided in the application notes with the publication numbers 5988 7831EN and 5988 7895EN With sequential Analysis 1 Regene Analysis 2 Regene Analysis 3 regeneration SEE o Ks gt G With alternating Analysis 1 Regene Analysis 3 regeneration
35. ries Valves 49 4 Valve Applications Solvent Selection Solvent Selection The Agilent 1200 Series 12 position 13 port valve can be used for solvent selection flow rate lt 10 ml min as illustrated in Figure 23 It offers automated access to 12 different eluents up to 12 bottles Figure 23 Solvent selection schematic setup 50 Agilent 1200 Series Valves Valve Applications 4 Solvent Selection Tubing Kit G1160 68706 for solvent selection 4 solvents with G1160A degasser and isocratic pump flow rate lt 10 ml min Table 15 Tubing From To Qty ID mm Length mm part number Solvent Bottle Degasser 4 1 5 1000 G1311 60003 Degasser Valve pos 1 12 4 15 600 G1160 67300 Valve OUT Pump Inlet 1 1 5 600 G1160 67300 Table 16 Finger tight fittings Description Qty part number Adapter PEEK int 1 4 28 to ext 10 32 5 0100 2298 Agilent 1200 Series Valves 51 4 Valve Applications Solvent Selection 52 Agilent 1200 Series Valves Agilent 1200 Agilent 1200 Series Valves User Manual 5 Parts General Parts 54 Capillaries and Fittings for analytical and preparative flow rates 56 Capillaries and Fittings for micro valves 57 In this chapter you will find part numbers and part descriptions for maintenance and repair dg Agilent Technologies 53 5 54 Parts General Parts Table 17 General parts Description part number e CAN cable 1m long 5181 1519 DC Can cable 5181 1533 Ext
36. t 1200 Series micro well plate autosampler G1377A e Agilent 1200 Series dual loop autosampler PS G2258A Table1 Site Requirements for Agilent 1200 Series Valves Type Specification e Weight 1 9 Kg 4 2 Ibs Dimensions 92 x 84 x 200 mm e height x width x depth 9 2 x 3 3 x 8 0 inches e Power supply 24 Volts DC 1 7 amps Ambient operating temperature 4 to 55 C 39 to 131 F e Humidity lt 95 non condensing Safety Standards IEC CSA UL EN Installation category II pollution degree 2 For indoor use only Table 2 System Requirements Revision Agilent 1200 Firmware 4 05 04 and higher Control Module Firmware G1323B B 03 11 and higher e Agilent ChemStation Software A 09 03 and higher 8 Agilent 1200 Series Valves Installation and Configuration 1 Unpacking the Agilent 1200 Series valve Unpacking the Agilent 1200 Series valve Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the Agilent 1200 Series valve may have been damaged during shipment CAUTION Ifthere are signs of damage please do not attempt to install the valve Delivery Checklist Ensure all parts and materials have been delivered with the Agilent 1200 Series valve The delivery checklists are displayed in Table 3 for G1157A G1158A G1159A G1160A and in Table 4 for G1162A G1163A
37. tem Errors and Troubleshooting 34 System Errors 34 Valve Synchronization 37 Agilent 1200 Series Valves 5 contents 4 Valve Applications Alternating Column Regeneration 40 Sample enrichment and Sample stripping 44 Sample enrichment 44 Sample stripping 45 Column Selection 48 Solvent Selection 50 5 Parts General Parts 54 Capillaries and Fittings for analytical and preparative flow rates 56 Capillaries and Fittings for micro valves 57 6 Specifications 7 Safety Information General Safety Information 66 Lithium Batteries Information 69 Radio Interference 70 SoundEmission 71 Solvent Information 72 Agilent Technologies on Internet 73 Index 75 6 Agilent 1200 Series Valves Agilent 1200 Agilent 1200 Series Valves User Manual 1 Installation and Configuration Site Requirements 8 Unpacking the Agilent 1200 Series valve 9 Hardware Installation 12 Software Configuration 14 dg Agilent Technologies 1 Installation and Configuration Site Requirements Site Requirements The requirements for the Agilent 1200 Series Valves are displayed Table 1 and Table 2 on page 8 The 24 Volts DC power can be supplied by an external power supply 0950 4422 or by one of the following Agilent 1200 Series LC modules with DC CAN adapter e Agilent 1200 Series preparative pump G1361A e Agilent 1200 Series fraction collectors G1364A G1364B G1364C G1364D e Agilent 1200 Series high performance autosampler G1367B e Agilen
38. tion after the run This behavior is exhibited in Figure 4 on page 19 Restore Starting Position Figure4 Valve Control Valve Name Define the Valve Name that is used for the method report and the Instrument actuals The Valve Name is limited to 20 characters Agilent 1200 Series Valves 19 2 20 Operation Operating the Valve using Agilent ChemStation Position Descriptions Define the Position Description that is used for the method report and the instrument actuals The Position Description is limited to 19 characters Table5 Available valve positions Valve Description Product Number of Positions Descriptions 2 position 10 port valve G1157A G1163A 2 2 position 6 port valve G1158A G1162A 2 6 position selection valve G1159A 6 12 position 13 port selection valve G1160A 12 Time Table The Time Table can be used to edit and run a valve program during a sequence of sample runs The Time Table contains 3 columns Line Time and Position The Time Table is limited to 20 lines The number of selection in the Position column depends on the valve that has been configured Position X Next Position Insert Append Cut Copy Paste switches the valve to the selected position The number of available positions for the Agilent 1200 Series valves is displayed in Table 5 switches to the next available position If the valve is on the highest position it will switch to position 1 Inserts a
39. tripping as displayed in Table 11 on page 46 and Table 12 on page 47 Capillary kit for sample enrichment stripping G1156 68714 with G1158A analytical scale Table 11 Capillaries From To ID mm Length mm part number Sample Enrichment Injector Autosampler Valve port 1 0 17 500 G1328 87600 Valve port 2 Waste 0 6 PTFE 2000 0890 1713 Valve port 3 Enrichment Column 0 17 400 5021 1819 Enrichment Column Valve port 6 0 17 600 5065 9933 Pump B Valve port 4 0 25 800 5065 9930 Valve port 5 Analytical Column 0 17 400 5021 1819 Analytical Column Detector 0 17 600 5065 9933 Stripping Injector Autosampler Valve port 1 0 17 500 G1328 87600 Valve port 2 Analytical Column 0 17 400 5021 1819 Analytical Column Detector 0 17 600 5065 9933 Valve port 3 Pre Column 0 17 400 5021 1819 Pre Column Valve port 6 0 17 600 5065 9933 Pump B Valve port 4 0 25 800 5065 9930 Valve port 5 Waste 0 6 PTFE 2000 0890 1713 Agilent 1200 Series Valves Valve Applications 4 Sample enrichment and Sample stripping Table 12 Fittings screws and ferrules Description Qty part number 1 16 fittings and ferrules 10 pk 1 5062 2418 fingertight fitting long 1 5062 8541 10 pk fitting screw long 5 5065 4454 10 pk front ferrule 10 pk 1 5180 4108 back ferrule 10 pk 1 5180 4114 ZDV fitting 1 0100 0900 0 18 ID PEEK tubing 1 5 m 1 0890 1763 Plastic tubing cutter 1 8710 1930 Agilent 1200 Series Valves 47 4 Valv
40. ubleshooting System Errors and Troubleshooting System Errors and Troubleshooting 34 System Errors No line power Valve 12PS 13PT Status Act Pos 1 Column 1 Name LC MSD Valve 12PS 13PT Status Act Pos 1 Column 1 Name LC MSD Figure 13 Display of instrument actuals at a no line power state Error Cause If the 24 volt DC line power is not available the yellow Notready status is displayed in the instrument actuals If you try to switch the valve this status changes to Power Actions 1 Check power cable to external power supply or to another Agilent 1200 Series module 2 If the valve power is supplied by another Agilent 1200 Series module check if this particular module is switched on 3 Restart the ChemStation Agilent 1200 Series Valves Maintenance Repair and Troubleshooting 3 System Errors and Troubleshooting CAN failure Valve 12PS 13PT Status Act Pos 1 Column 1 Name LC MSD Figure 14 CAN failure Error Cause If the red Pow fail status is visible the CAN communication is interrupted Actions 1 Check the CAN connection at the valve and at all other modules 2 Check that the maximum number of 13 Agilent 1200 Series modules including CAN slaves is not exceeded 3 Restart the ChemStation Switch failure Valve 12PS 13PT Status Bpen Failure Act Pos 9 Name LC MSD Figure 15 Switch failure Error Cause The yellow failure status indicates that th
41. ump B is equilibrating the analytical column After the valve is switched to position 2 pump B is backflushing the sample on the analytical column for separation and subsequent detection by LC MS or optical detectors Agilent Technologies offers capillary kit for sample enrichment and sample stripping The content is listed in Table 11 on page 46 and Table 12 on page 47 Sample stripping Sample stripping is another method to remove complex sample matrices In difference to sample enrichment the matrix is trapped on the pre column while the analytes pass through onto the analytical column Pump Autosampler Analytical column Detector Position 1 C Pump B Pump A Autosampler Detector Position 2 Figure 21 Sample stripping schematic setup Agilent 1200 Series Valves 45 4 46 Valve Applications Sample enrichment and Sample stripping Figure 21 on page 45 illustrates how the Agilent 1200 Series 2 position 6 port valve can be used for sample stripping In valve position 1 Pump A transfers the complete sample matrix onto the pre column 1 where the matrix is trapped while the analytes are eluted and flushed to column 2 for analysis Then the valve switches to position 2 Now pump B backflushes the matrix to the waste while pump A continues to deliver solvent to the analytical column where the analytes are separated and analyzed Agilent Technologies offers a capillary kit for sample enrichment and s
42. ve fittings t The consumable kit 5065 4410 contains two M 4 micro valve plugs The M4 micro valve fittings support the Agilent PEEK coated fused silica capillaries O D 0 8mm or 1 32 The M4 micro valve plug is supplied for pressure leak testing Agilent 1200 Series Valves Installation and Configuration 1 Unpacking the Agilent 1200 Series valve For a list of recommended Agilent PEEK coated fused silica capillaries can be found in the chapter Capillaries and Fittings for micro valves on page 57 Agilent 1200 Series Valves 11 1 Installation and Configuration Hardware Installation Hardware Installation The Agilent 1200 Series valves can be installed in two different ways They can either be placed on free bench space near the Agilent 1200 Series LC system or they can be mounted on the G1383A Agilent 1200 Series column organizer The column organizer should be located on the right side of the Agilent 1200 Series stack which includes the autosampler In order to install a valve on the G1383A Column Organizer the Organizer Rail Assembly must be installed on the two mounting poles using the clips from the Organizer Rail Assembly All External Valves have a bracket on the side which slides over the metal part of the Organizer Rail Assembly Two CAN connectors and one DC CAN connector are located at the back panel of the valve assemblies as displayed in Figure 1 Free 0 CAN DC Out
43. ves and provides feedback when a user settable limit is exceeded The visual feedback in the graphical user interface provides an indication that the exchange of the stator face and or the rotor seal should be scheduled Memo Pad EMF Info Pad VLV VLV3 G11574 LP0000003 Limit Actual Valve switches 50000 E Figure 12 Early Maintenance Feedback EM F In the Diagnostics view you can define after how many valve switches the stator face and or the rotor seal should be replaced The setting for the EMF limit depends on analytical conditions e g use of buffer solutions Hence the setting should be optimized over one or two maintenance cycles Setting the EMF limit 1 Switch to the Diagnostics View of the ChemStation 2 Click on the EMF icon The EMF Info Pad Figure 12 will appear on the right panel of the user interface 3 Select a valve on the dropdown list of the EMF Info Pad 4 Enter the EMF limit and click on Send Changes Agilent 1200 Series Valves Maintenance Repair and Troubleshooting Maintenance and Repair Resetting the EMF counter 1 Switch to the Diagnostics View of the ChemStation 2 Select Valve from the Maintenance menu 3 In the upcoming dialog box click on Reset Counter see also Figure 17 on page 37 The procedure to reset the EMF counter with the Agilent 1200 Series control module is described on page 23 Agilent 1200 Series Valves 3 33 3 Maintenance Repair and Tro
44. w can be reduced Table 28 G1160A Agilent 1200 Series 12 position 13 port selection valve Liquid contacts Stainless steel and PEEK Port size Accepts 10 32 male threaded fittings Flow passage diameters 1 0 mm 0 040 Volume in flow passage Stator includes stator face seal 6 4 ul hole rotor seal 4 0 ul groove Maximum pressure 21 M Pa 207 bar 3000 psi Recommended flow range 0 2 100 ml min at high pressures after the pump 0 2 10 ml min at low pressures in front of the pump Table 29 G1162A Agilent 1200 Series 2 position 6 port micro valve Liquid contacts DuraLife processed stainless steel stator and vespel rotor seal Port size Accepts M 4 male threaded fittings Flow passage diameters 0 20 mm 0 008 Volume in flow passages 70 nl port to port Maximum pressure 41 M Pa 408 bar 6000 psi Recommended flow range 0 1 100ul Table 30 G1163A Agilent 1200 Series 2 position 10 port micro valve Liquid contacts DuraLife processed stainless steel stator and vespel rotor seal Port size Accepts M 4 male threaded fittings Flow passage diameters 0 20 mm 0 008 Agilent 1200 Series Valves 63 6 64 Specifications Table 30 G1163A Agilent 1200 Series 2 position 10 port micro valve continued Volume in flow passages Stator 20 ports 27 2 nl 45 ports 30 5 nl rotor seal 25 0 nl groove Maximum pressure 41 M Pa 408 bar 6000 psi Recommended flow range 0 1 100ul
45. xt run after the current run is finished A flashing red status indicator indicates a severe error during the startup procedure of the module Call your local service provider for assistance upon observing this error condition Agilent 1200 Series Valves Maintenance Repair and Troubleshooting 3 Maintenance and Repair Maintenance and Repair The maintenance of the Agilent 1200 Series valves G1157A G1158A G1159A G1160A includes the exchange of the stator face and the rotor seal In addition it might be necessary to exchange the stator head if the threads are worn out or if a fitting is broken and cannot be removed from the stator head In contrast to the valves for standard flow rates the valve head assemblies of the Agilent 1200 Series micro valves G1162A and G1163A don t contain a stator face Only the rotor seal needs to be exchanged during maintenance The valve head assemblies are displayed in Figure 9 on page 28 Figure 10 on page 29 and Figure 11 on page 30 Part numbers of the rebuild kits rotor seals and stator heads are listed in Table 6 on page 31 Always rinse the valve with water after using aqueous buffers or salt solutions to prevent crystal formation which may damage the valve Agilent 1200 Series Valves 27 3 Maintenance Repair and Troubleshooting Maintenance and Repair Replacing the stator face and the rotor seal for standard valves G1157A G1158A G1159A G1160A Disassembling the valve hea
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