eBlot user manual
Contents
1. Salt built up on plate electrodes 2 Membrane insufficiently equilibrated in eBlot Equilibration buffer 3 Incorrect transfer conditions or insufficient transfer time 4 PVDF membrane was not prewet with methanol 5 Confusion of the eBlot Anode Pad and Cathode Pad Air bubbles trapped between gel and membrane prevent the transfer of proteins 21 www genscript com Solution Ensure the transfer stack is assembled correctly use the eBlot Anode Pad first followed by the membrane the pre run gel Gel Window and eBlot Cathode Pad Check the transfer stack and ensure Gel Window covered correctly on the gel 1 Clean the titanium cathode plate graphite anode plate with a wet cloth or paper tissue followed by a dry one to remove any insoluble salts 2 Equilibrate membrane in eBlot Equilibration buffer before transfer 3 Use a gel of lower concentration to separate high molecular weight proteins Increase the transfer time in 5 second increments 4 Prewet PVDF membrane with methonal before transfer 5 Ensure the transfer stack is assembled correctly Bottom eBlot Anode Pad yellow Top eBlot Cathode Pad white When assembling transfer stack use the small shovel supplied with the device to remove any air bubbles between the gel and the membrane eBlot Protein Transfer System Examples of Results Westen blot result after using the eBlot for protein tr2. Electrode L03012 For best results when a graphite electrode has been used for 100 times of protein transfer replace it with a new one eBlot Equilibration Buffer The eBlot Equilibration Buffer M01078 125 ml is available separately from GenScript Product Cat No eBlot Equilibration Buffer M01078 D Important DO NOT start a run without properly assembled Transfer Stacks in place eBlot Protein Transfer System www genscript com Product Specifications Intended Use For research use only Not intended for human or animal diagnostic or therapeutic uses eBlot Protein Transfer Weight 1 6 kg Device Specifications Dimensions 325 mm I x195 mm w x70 mm h Electrical Parameters 100 120 V 220 240 V 50 60 Hz 3 2 A Built in Features Digital Display Alarm Light LED Compatibility Suitable for fast electroblotting of proteins from mini polyacrylamide gels to PVDF or nitrocellulose membranes Materials Acrylonitrile Butadiene Styrene Polycarbonate Aluminum Titanium Plasticized silicone Operating Temperature 15 40 C Forceps Stainless steel Shovel Polycarbonate Shallow Tray Polycarbonate Avoid acetone dimethyl sulfoxide and acetic acid These reagents can erode or damage the device eBlot Protein Transfer The eBlot Protein Transfer Pads are used with the eBlot Protein Transfer Device Pads Specifications The specifications of the eBlot Protein Transfer Pads are listed below
3. Stack 5 Tear the sealing of the Nitrocellulose or PVDF Membranes package Remove one sheet of membrane and soak it in the eBlot Equilibration Buffer for 1 minute Note If a PVDF membrane is used the membrane must be pre wetted with methanol before equilibrating in eBlot Equilibration Buffer For users of eBlot Protein Transfer Pads Basic 20 pak L03011 the precut membrane should be prepared by themselves 13 dd eBlot Protein Transfer System www genscript com eBlot Protein Transfer System www genscript com Protocols continued Protocols continued Assembling the Transfer 6 Place the equilibrated membrane on the eBlot Anode Pad Gently remove Assembling the Transfer 9 Select appropriate Gel Window according to actual size of the gel see Stack continued air bubbles between the membrane and the anode pad using the small Stack continued the table below Place Gel Window on the gel Ensure that the Gel shovel supplied with the device Window fully covers the margin of the membrane Pre run gel Gel Window Gel with size gt 80 x 60 mm Gel Window 88 x 78 mm Gel with size 66 x 56 mm to 80 x 60 mm Gel Window 76 x 64 mm Gel with size 66 x 56 mm Gel Window 66 x 56 mm Note Gel Window is used as the spacer between anode pad and cathode pad to prevent short circuit 7 Carefully remove the pre run gel containing your protein samples from the gel cassette and briefly rinse the gel with distilled water
4. device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference Tested By as SEM Test Compliance Service Co Ltd 3 F Jinbao Commerce Building Xin an Fanshen Road The Certification of Verification shows that the tested sample technically compliances with the FCC Part 15 The certification applies to the tested sample above mentioned only and should not implied an assessment of the whole Tel 86 755 33663308 Fax 86 755 33663309 E mail sem semtest com cn Website www ye www genscript com
5. eBlot Protein Transfer Pad eBlot Anode Pad 90 mm lI x 80 mm w x 2 5 mm thickness eBlot Cathode Pad 90 mm 1 x 80 mm w x 2 5 mm thickness Materials Blotting filter paper presoaked with proprietary anode or cathode buffer Membrane Nitrocellulose 90 mm 1 x 80 mm w PVDF 90 mm I x 80 mm w eBlot Equilibration Buffer Size 125 ml x 2 Formulation Proprietary eBlot Protein Transfer System www genscript com Product Specifications continued eBlot Graphite Electrode Specifications Gel Window Inter frame size 66 mm x 56 mm 76mm x 64 mm 88 mm x 78 mm Materials Polycarbonate Absorbent Filter Paper Size 80 mm x 70 mm Materials Vegetable fiber The eBlot Electrode is used as the replaceable anode electrode of eBlot Protein Transfer Device and available separately from GenScript The specifications for eBlot Graphite Electrode are listed below eBlot Graphite Electrode Dimensions 105 mm I x 95 mm w x 10 mm h Weight 140 g Materials Powdered carbon Clay and Stainless steel E eBlot Protein Transfer System www genscript com eBlot Protein Transfer Device Front View of eBlot Device Rear View of eBlot Device The front top view showing various parts of the eBlot Protein Transfer Device is shown below mmm Control Panel Open Button The rear view showing various parts of the eBlot Protein Transfer Device is shown belo
6. GenScript Transforming Biology Research GenScript USA Inc 860 Centennial Ave Piscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email product genscript com eBlot Protein Transfer System For fast semi dry electroblotting of proteins from mini polyacrylamide gels to nitrocellulose or PVDF membranes Version No 09192012 User Manual GenScript Transforming Biology Research eBlot Protein Transfer System www genscript com Table of Contents PIOGUCl COnDIeHIS EA 1 Product SPECIOZA 3 eBIOE Proein DEANISIET IDOVIGO AI de 5 Accessory PrOdUCIS eestessessoerpdenescesoeedeeecedueres esten ge ase seer meet 7 IMMOQUGHON iii HA 8 UIC IREIGIENCe UA AA 11 l IDIOGDIS Ai 12 TTOUDIESNOOUNO AI 21 BAA ui AA aad ach 22 Technical SUDDOLE iii iii EEEH EA EA dean te 23 WANI c aa 24 Kan Aaaa 25 eBlot Protein Transfer System Product Contents Type of products eBlot Protein Transfer Device Contents Upon Receiving the Instrument eBlot Protein Transfer Pads www genscript com This manual is supplied with the eBlot Protein Transfer Device L03010 The contents of the eBlot Protein Transfer Device are listed below Component Quantity eBlot Protein Transfer Device 1 each eBlot Graphite Electrode Installed inside the device 1 each Sponge Cushion Installed inside the device 2 each Regional specific power cord 1 each Forceps 1 each Shove
7. Transfer System Accessory Products Precast Gels and Premade Buffers ONE HOUR Western Detection Kits www genscript com The precast Express PAGE Gels as well as premade buffers and buffer powders are available from GenScript For details contact Technical Support or visit www genscript com The ONE HOUR Western Detection Kits used for Western blotting analysis are available from GenScript Ordering information is provided below For more information visit www genscript com or call Technical Support Product ONE HOUR Western Basic Kit Rabbit ONE HOUR Western Basic Kit Mouse ONE HOUR Western Basic Kit Goat ONE HOUR Western Standard Kit Rabbit ONE HOUR Western Standard Kit Mouse ONE HOUR Western Standard Kit Goat ONE HOUR Western Standard Kit with TMB Rabbit ONE HOUR Western Standard Kit with TMB Mouse ONE HOUR Western Standard Kit with TMB Goat ONE HOUR Western Advanced Kit Rabbit ONE HOUR Western Advancedkit Mouse ONE HOUR Western Advanced Kit Goat ONE HOUR IP Western Kit Rabbit ONE HOUR IP Western Kit Mouse ONE HOUR IP Western Kit Goat ONE HOUR Western Fluorescent Kit ONE HOUR Western Multiplex Fluorescent Kit Quantity 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Assays 1 Kit 5 Ass
8. al number of your instrument For your protection items being returned must be insured against possible damage or loss This warranty shall be limited to the replacement of defective products It is expressly agreed that this warranty will be in lieu of all warranties of fitness and in lieu of the warranty of merchantability 24 eBlot Protein Transfer System www genscript com eBlot Protein Transfer System www genscript com Appendix Appendix continued Ec Ie Cer Z VZTZFZTe Center Europe tion Service Certificate No CECS 0020110523A Certificate No CECS 0020110523B VERIFICATION OF EMC COMPLIANCE _ VERIFICATION OF LVD COMPLIANCE E fu pu hae EU COUNCIL DIRECTIVE 2004 108 EC President of CECS en 4 President of CECS Center Europe Certification Service Co Ltd Abbey Orchard St Westminster Greater London SW1P z infogcersuk com 25 26 eBlot Protein Transfer System Appendix continued VERIFICATION OF CONFORMITY According to FCC Part 15B Certificate No SEM11058591 Responsible Partys Name NanJing Genscript Co Ltd Address No 78 Shuangbai Road Xuanwu District Nanjing China Manufacturer 1 Naming Genscript Co Ltd Address No 78 Shuangbai Road Xuanwu District Nanjing China Description of Product Multifunction Gel Processor Trade Name Stain eBlot Report No TR11058051E 3 Compliance With Part 15B of FCC Rules This
9. ansfer Result Using Nitrocellulose www genscript com a Tubulin 55 kDa B Actin 42 kDa GAPDH 36 kDa Hela cell lysate ug 10 5 25 125 0 62 0 31 0 16 An Express PAGE Gels 8 16 GenScript MG816W12 was blotted using the eBlot 10 0 5 0 2 5 1 25 0 62 0 31 and 0 16 ug of HeLa cell lysate were loaded respectively Proteins on the Nitrocellulose membrane were detected using THE alpha Tubulin Antibody GenScript A01410 THE beta Actin Antibody GenScript A00702 and GAPDH Antibody GenScript A01622 The secondary antibody was Anti MOUSE IgG H amp L GOAT Antibody Peroxidase Conjugated Rockland 610 1302 The signal was developed with LumiSensor HRP Substrate Kit GenScript L00221V500 Good transfer of protein standard bands onto the nitrocellulose membrane Protein Standards were separated on a 4 20 Genscript Express Plus PAGE Gel Bis Tris After electrophoresis the gel was blotted using the eBlot protein transfer system for 11 min as described in this manual Lane 1 and 2 Invitrogen Pre Stain protein marker 5 uL MW 4 250 kDa Lane 3 and 4 Bio rad Pre Stain protein marker 10 uL MW 10 250 kDa and Lane 5 9 NEB All Blue Pre Stain protein marker 0 3 0 6 1 3 2 5 and 5 uL MW 10 230 kDa 229 eBlot Protein Transfer System Technical Support Web Resources Contact Us www genscript com Visit the GenScript Web site at www genscript com for 1 Technical
10. at allows for fast transfer of proteins eBlot Protein Transfer Pads As the consumable part of eBlot Protein Transfer System the eBlot Protein Transfer Pads are the mixed assortment of eBlot Protein Transfer Pad nitrocellulose or PVDF membranes Equilibration Buffer and Gel Window Each pack of eBlot Protein Transfer Pad contains an 1x eBlot Cathode Pad and an 1xeBlot Anode Pad presoaked with proprietary cathode buffer and anode buffer respectively allowing for rapid convenient and reliable protein blotting without the need to prepare additional buffers eBlot Protein Transfer System www genscript com eBlot Protein Transfer System www genscript com Introduction continued Introduction continued System Mechanism The eBlot Protein Transfer System is based on the proprietary fast semi dry System Features Important features of the eBlot Protein Transfer System are listed below Transferred protein sample blotting technique developed by GenScript To use the eBlot Protein Transfer System for rapid protein blotting assemble the eBlot Protein Transfer Pad with your pre run gel and the membrane on the eBlot Protein Transfer Device The eBlot Cathode Pad and eBlot Anode Pad act as ion reservoirs that contain the appropriate anode and cathode buffers The design of the eBlot Protein Transfer Device reduces the distance between electrodes and the integrated power supply enables the system to gen
11. ays 1 Kit 5 Assays 1 kit 5 Assays 1 Kit 10 Assays 1 Kit 10 Assays Cat No L00204 L00205 L00399 L00204C L00205C L00228 L00204T LOO205T L00228T L00241 L00242 L00243 L00231 L00232 L00233 L00397 L00398 eBlot Protein Transfer System Introduction System Overview System Components www genscript com Semi dry Western blotting is a common technique applied in protein research Conventional semi dry blotting is a cumbersome process requiring time consuming reagent preparation and setup followed by an electrophoretic transfer that could take one hour or more GenScript s eBlot Protein Transfer System accelerates the semi dry blotting process without sacrificing performance The eBlot Protein Transfer System consisting of the eBlot Protein Transfer Device and eBlot Protein Transfer Pads enables researchers to quickly reliably perform electrophoretic transfer of proteins from various types of mini polyacrylamide gels to membranes in 7 to 10 minutes without the need to prepare additional buffers The proteins transferred using the eBlot Protein Transfer System exhibit high detection sensitivity as to proteins transferred using other existing blotting methods The eBlot Protein Transfer System consists of eBlot Protein Transfer Device The eBlot Protein Transfer Device is a self contained electroblotting unit with a power supply built in th
12. best results follow these recommendations T Wear gloves at all times during the entire blotting procedures to prevent contamination of pads gels and membranes Mode Action Sound Light Display eBlof Device Connect eBlot B Steady right Default 2 Do not touch the gel or membrane with bare or gloved hands plugged in Device to an electrical light running This may contaminate the gel or membrane and interfere with further outlet and power time 00 00 analysis If needed always use forceps to adjust the membrane or gel switch is on 3 Avoid using expired eBlot Protein Transfer Pads and eBlot Equilibration eBlot Device and Assemble transfer steady right Default Buffer Always use the pads and buffer before the specified expiration date transfer stack stack on the device light running printed on the package assembled and close lid time 00 00 l j l 4 Remove any trapped air bubbles between the gel and membrane during Time selection Press Min and Sec Steady right User specified the assembly of the transfer stack using the small shovel supplied with the button to select light running desired running time time 00 00 device Run Press Start Stop Flashing right Counting down button light time Installing the 1 Check the Power Cord supplied with the unit to ensure that the cord is End of run Automatic Continuous beeping Steady right Default running eBlot Device compatible with the local socket format for 2 minu
13. eBlot Protein Transfer Pad Note Do not re use the eBlot Protein Transfer Pad after blotting Discard after each use 4 Clean the titanium cathode plate graphite anode plate and its surrounding area with a dry cloth or paper tissue 5 Replace the Absorbent Filter Paper in the Waste Tray with a new one when it has soaked up the waste from 20 times of transfer Pull out the Waste Tray from the right side of the device to perform the replacement Note After about 40 runs take out the graphite electrode soak in distilled water for 30 minutes dry the surface with a clean paper towel then leave air dry overnight with graphite side up At this point the eBlot Protein Transfer Device is ready for another run If you are not using the device turn off the Power Switch located on the back of the device For any other repairs and service contact Technical Support Do not perform any repairs or service on the eBlot Protein Transfer Device by yourself to avoid any possible damages to the device 18 eBlot Protein Transfer System Protocols continued Replacing the Graphite Electrode www genscript com During the transfer process the Graphite Electrode will absorb ions from anode pad as well as lose carbon composition of the anode buffer For best blotting results after having been used for 100 times of transfer the worn Graphite Electrode should be replaced by a new one 1 Ifthe eBlot Protein Trans
14. erate a certain definite voltage allowing for rapid and directional movement of negatively charged protein molecules from the gel matrix onto the membrane within 7 10 minutes Meanwhile the membrane presoaked with eBlot Equilibration Buffer shows good combination ability with proteins that prevents the flow through of low molecular weight proteins from the membrane Schematic mechanism of eBlot Protein Transfer System showing the flow of current Titanium Cathode 2e 2H O gt H 20H eBlot Cathode Pad i aa Pre run gel l I I I I i I I l I Membrane l eBlot Anode Pad l Graphite Anode 4OH 4e O 2H O Unique semi dry electroblotting technique created for fast reliable protein transfer within 7 10 minutes Self contained electroblotting unit with an integrated power supply for easy and convenient procedures Consumable transfer pads offering convenience without the need for additional buffers Compatible for use with various types of mini polyacrylamide gels Proprietary formula without methanol High transfer efficiency as compared to other existing blotting methods 10 eBlot Protein Transfer System www genscript com eBlot Protein Transfer System www genscript com Quick Reference Guide Protocols A quick reference guide for operating the eBlot Protein Transfer Introduction Recommendations Device is provided below To obtain the
15. fer Device works at transferring mode press and hold Min button for 2 seconds to toggle to numbering mode If the upper three digits show 100 or a number greater than 100 perform the replacing protocol as describe below 2 Switch Off the eBlot Protein Transfer Device Open the lid of the device and take the worn Graphite Electrode out of the device 3 Tear the sealing of a new Graphite Electrode package and take the new Graphite Electrode out of the package Place the new Graphite Electrode into the anode tank and close the lid of the device 19 eBlot Protein Transfer System www genscript com Protocols continued Replacing the Graphite Electrode continued 4 Switch On the eBlot Device Press and hold Min button for 2 seconds to toggle to numbering mode When the upper three digits are flashing press Reset button to zero the transferring times After successfully installing Graphite Electrode into the eBlot Device you are ready to use the eBlot Device for another blotting application D0 eBlot Protein Transfer System Troubleshooting Problem The right Status Light doesn t flash during blotting process The left and right Status Lights flash simultaneously Inefficient transfer Empty spots on the membrane Cause Incomplete electric circuit due to improper assembly of the transfer pads Excessive current is flowing through the Device 1
16. in another shallow tray to remove any small gel pieces attached to the gel and facilitate easy positioning of the gel on the membrane 8 Place the gel on the membrane Gently remove air bubbles between the 10 Tear the sealing of the 1xeBlot Cathode Pad package Remove the gerand Te membrane eBlot Cathode Pad from the package and place it on the gel Note During assembling of the transfer stack make sure to remove all the air bubbles trapped between the transfer pads pre run gel and the membrane which may prevent the transfer of proteins and cause empty spots on the transferred membrane 15 16 eBlot Protein Transfer System Protocols continued Assembling the Transfer Stack continued Performing Blotting www genscript com 11 Press the Open button and then push back and close the lid of eBlot Protein Transfer Device Perform protein blotting as described below within 15 minutes of assembling the transfer stack 1 Press the Min and Sec buttons to set appropriate running time based on the protein size see table below If an undesired running time is set by mistake press Reset button to clear the wrong time and then press the Min and Sec buttons to choose the desired running time Recommended Protein size kDa start running time min lt 80 7 80 160 8 9 gt 160 10 Note Based on the initial results the transfer time may need to be optimized to make best tran
17. inued Installing the 7 Pull out the Waste Tray from the right side of the device Place a new Assembling the Transfer 2 Remove one package labeled as eBlot Protein Transfer Pad from the eBlot Device continued Absorbent Filter Paper inside the tray and then push the tray in Stack continued eBlot Protein Transfer Pads box and tear the laminated sealing of the Note Change absorbent paper with a new one when opening a new box of pads package Remove the two small packages respectively labeled 1xeBlot Cathode Pad and 1xeBlot Anode Pad i Blot a 8 Attach the power cord to the AC inlet and then to the electrical outlet 3 Tear the sealing of the 1xeBlot Anode Pad package Remove the Use only properly grounded AC outlets and power cords eBlot Anode Pad from the package and place it on the anode plate of 9 When the electrophoresis of your samples is almost complete press the eBlot Protein Transfer Device the Power Switch located on the rear of the device to turn ON the eBlot Protein Transfer Device The right Status Light is on indicating you are using transfer mode The lower four digits of the Digital Display show the default running time 00 00 You are ready to use the eBlot Protein Transfer Device for blotting application 4 Pour 10 ml eBlot Equilibration Buffer into the shallow tray supplied with the eBlot Device Assembling the Transfer 1 Open the closed lid by pressing the Open button
18. l 1 each Shallow Tray 2 each Examine the unit carefully for any damage incurred during transit File any damage claims with the carrier The warranty does not cover in transit damage The following eBlot Protein Transfer Pads are available from GenScript Product Cat No eBlot Protein Transfer Pads L03011 Basic without membrane 20 pak eBlot Protein Transfer Pads Nitrocellulose 20 pak L03013 eBlot Protein Transfer Pads PVDF 20 pak L03014 The eBlot Protein Transfer Pads come with the following components Component Basic Nitrocellulose PVDF eBlot Protein Transfer Pad 20 20 20 Nitrocellulose membranes 20 sheets 1 PVDF membranes 20 sheets eBlot Equilibration Buffer 125 ml Gel Window 66 mmx56 mm Gel Window 76 mmx64 mm Gel Window 88 mmx78 mm Absorbent Filter Paper N nP N nPK N n nNi Sponge Cushion eBlot Protein Transfer System www genscript com Product Contents continued Components for eBlot Protein Transfer Pad are as follows Component Quantity 1x eBlot Cathode Pad 1 1x eBlot Anode Pad 1 Store the eBlot Protein Transfer Pads at room temperature For best results use the eBlot Protein Transfer Pads before the expiration date printed on the package eBlot Graphite Electrode The eBlot Graphite Electrode L03012 is available separately from GenScript Product Cat No eBlot Graphite
19. resources including manuals vector maps and sequences application notes MSDSs FAQs formulations citations handbooks etc 2 Complete technical support contact information 3 Access to the GenScript Online Catalog 4 Additional product information and special offers For more information or technical assistance call write fax or email GenScript USA Inc 860 Centennial Ave Piscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email product genscript com x09 eBlot Protein Transfer System Warranty eBlot Protein Transfer Device www genscript com GenScript warrants that eBlot Protein Transfer Device will be free from defects in material and workmanship for a period of one year from date of purchase If any defects occur in the product during this warranty period GenScript will at its option repair replace or refund the purchase price of this product at no charge to you The following defects however are specifically excluded 1 Defects caused by improper operation 2 Repair or modification done by anyone other than GenScript or an authorized agent 3 Use of fittings or other spare parts supplied by anyone other than GenScript 4 Damage caused by accident or misuse 5 Damage caused by disaster 6 Corrosion due to use of improper solvent or sample For any inquiry or request for repair service contact GenScript after confirming the model and seri
20. sfer results by pressing the Time button in 5 second increment For proteins greater than 200 kDa we recommend to start with 11 minutes 2 Press the Start Stop button to start the transfer The running time begins to count down and right Status Light keeps flashing during the whole transfer program siTe eBlot Protein Transfer System Protocols continued Performing Blotting continued Disassembling and Cleaning the eBlot Device www genscript com 3 At the end of the transfer current automatically shuts off and the eBlot Protein Transfer Device signals the end of transfer with repeated beeping sound The right status light stops flashing and the lower five digits show text 00 00 4 Press any button on the control panel to stop the beeping 5 Proceed to disassemble the stack and clean the device To obtain good transfer and detection results disassemble the transfer stack right away after ending the blotting procedure 1 Open the closed lid by pressing the Open button 2 Carefully separate the transferred membrane from the transfer stack and proceed with further protein detection procedures Note If you are using PVDF membrane place the membrane immediately into the blocking solution or water as PVDF membrane dries quickly If the PVDF membrane is dried rewet the membrane with methanol and then rinse it with distilled water to wet it completely before use 3 Discard the gel and the used
21. tes light time 00 00 l l 2 Place the eBlot Protein Transfer Device on a levelled laboratory bench Checking the number Press and hold Min Steady left Times of the i tilati f th auses orie butono aececi aadis graphie anode Keep the area around the device clear to ensure proper ventilation of the graphite anode has been used unit for transfer 3 For your safety Position the device properly such that the Power Switch Replacement of Switch off the device z and the AC inlet located on the rear of the unit are easily accessible worn graphite and replace the worn 4 Ensure the Power Switch is in the Off position anode grapnite nese Wina 5 Open the closed lid of the eBlot Protein Transfer Device by pressing new one 24 the Open button Place one or two pieces of Sponge Cushion in the anode tank depending on gel thickness For 1 5 mm gel use one piece of Sponge Cushion for 0 75 and 1 0 mm gel use two pieces of Sponge Cushion Note After 20 times of protein transfer replace the used Sponge Cushions with new 6 ones A pair of new Sponge Cushions are included in each box of eBlot Protein Transfer Pads Insert the eBlot Graphite Electrode into the anode tank as described in Section Replacing the eBlot Graphite Electrode then close the lid of the device s42 eBlot Protein Transfer System www genscript com eBlot Protein Transfer System www genscript com Protocols continued Protocols cont
22. w AC Inlet Power Switch eBlot Protein Transfer System www genscript com eBlot Protein Transfer Device continued Control Panel of eBlot Device Top View of Open eBlot Device The control panel of the eBlot Protein Transfer Device is described below The Digital Display shows two rows of multi digits that specify the transfer conditions as follows The upper three digits after text lt PN gt indicate how many times of transfer the graphite electrode has been used for The lower four digits specify the running time of protein transfer in minute and second The two Status Lights show the working mode of the eBlot Protein Transfer Device When the right status light is on the device is switched on and working at transferring mode when both left and right status lights are on the device is working at numbering mode The Reset button is used to clear parameters The Min button is used to shift between transferring and numbering mode and to set running time Each short press will increase one minute Each long press 2 seconds will toggle working mode from transferring to numbering or the opposite The Sec button is also used to set running time each press will increase 5 seconds The Start Stop button is used to activate stop the transfer program The top view of open eBlot Protein Transfer Device identifying various parts Titanium Cathode Graphite Anode eBlot Protein
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