Tracking Tool™ PRO v2.0
Contents
1. Tracking Tool PRO software was developed together with High Performance Consulting www hpcsweden se based on specifications by Gradientech AB Gradientech AB Uppsala Science Park SE 751 83 Uppsala Sweden Phone 46 18 418 6700 www gradientech se sales gradientech se 2015 10 232. mean min max std Accumulated distance um 414 50 2137 327 Euclidean distance pm 149 00 1527 204 Velocity um min 28 fo2 83 27 Centre of mass um 226 76 81 Forward migration index 0 06 024 Directness Rayleigh test p value 9e 025 Data Explanation Migration Directions This chart shows the number and percentage of cell migrating either to the left or right x and x and down or up y and y px x oy ty Number of cells migrating Percentage of cells migrating Migration Distances mean min Max sta Euclidian distance d Accumulated distance um 55 7 Euclidean distance um 1580 36 0 Velocity um min 29 5 108 8 Accumulated distance 6 The accumulated distance is the total length of the track in um Euclidian distance d The euclidian distance is the dis tance between the track start and end point in um Accumulated distance d 20 Step by Step Tutorial Ba bs FE Velocity cucimean distance pm 4 0 Velocity um min Velocity um min The mean migration velocity of all selected tracks in um min The centre of mass is the mean end point value of the tracked cells Centre of Mass and Forward Migration Index Centre of mass pm 103 3 Forward index 5 0012 Centre of mass um Forward migration index The forward migration index is the directness see be
3. keyboard Tracking Show path Show labels Add track Delete track Screenshot of the Tracking settings panel In this ex ample tracks were selected by clicking on Track 4 then left clicking on Track 7 while holding the Shift key End track Adding Ending and Deleting Tracks Add track Click Add track to start a manual track Hover the mouse cursor over the cell you wish to track and left click After every click the frame slider advances to the next frame Continue clicking until you wish to end your track or have reached the last frame End track If you wish not to continue tracking a cell or if a cell has disappeared from the image you can end the track by clicking the End track button The position data from the prematurely ended track will be included in the statistical analysis and the final plot Delete track By clicking Delete track you erase the selected track and all its position data A deleted track will not be included in the statistical analysis and in the final plot Tracking Hotkeys It is highly recommended to use the keyboard hotkeys that are implemented into Tracking Tool PRO in combination with the mouse Use one hand on the mouse to point at the cell and the other for the hotkeys for adding track points and for adding ending and deleting tracks In this way you can track your cells faster more accurately and with much less fatigue to your hand and wrist compared to using the mouse on
4. plots gt Logout Tracking Settings Analysis Plotting Merge plots My account Data settings 0 degrees 0 609 um pixel Get from image 1 Time between frames min Apply settings Change settings Track selection Selected tracks 507 582 Tracks shorter than lo um Screenshot of the Settings tab The rectangle selection tool is seen here selecting an area of tracked cells to be used in later data analysis and plotting In this example the area in the middle has been selected track labels are shown in green Data Settings Statistical Input Parameters Rotating Image Data Set your image sequence was unintentionally rotated during the experimental set up you are able to cor rect this here Move the slider until for example the middle line or a channel wall in the gradient channel lines up to the background grid You can either drag the slider with the mouse or use the left or right ar row key on the keybord for fine adjustments 17 Step by Step Tutorial um to pixel Ratio Microscopes will output images with different um to pixel ratios depending on the microscope sensor and objective used Determining the um to pixel value of your microscope objective combination is im portant to get correct statistical values and plots Below you will find a method for determining this ratio when using CellDirector 2D Once determined you can re use the um
5. the same as your email address Username eopage com Password Re type password Name Company org Phone number 8 create account Note Only professional emails are allowed as user name 6 User Account My Account My account is a new tab in Tracking Tool PRO In My account tab you can view and edit your account information order tickets to access automated tracking and view your ticket purchase log Mo Tracking Tool PRO V2 0 unsaved project Tracking Settings Analysis Plotting Merge plots My account Account information Name Username Company Phone number Available tickets Creation date Edit account information Johanna info gradientech se Gradientech 0046184186700 9 12 2 2014 9 16 14 AM Get more tickets Pending ticket requests 50 tickets requested at 12 2 2014 10 07 26 AM Request trial tickets Edit Account Information By clicking Edit account information you are able to change your personal settings such as User name Password Name Company and Phone number Username johanna spaak gradientech se ex john webpage com Password Re type password eones Name Johanna Company org Gradientech Phone number gt 46 0 70 Ticket System Automated tracking is one of the new great features in Tracking Tool PRO that will save you significant amount of time tracking your cells To access automated traking you will need tickets To order t
6. to pixel value when analysing image sequences captured with the same microscope objective combination 1 Rotate the image so that it is correctly aligned to the background grid 2 Click Get from image 3 Click on the start point drag to the end point of measure and release the mouse button 4 In the pop up window enter the real distance of your object in um Finally click OK y Please provide inpu After the end point has been selected a pop up window shows up asking for the real distance in um A value of 500 um is assigned which is the width of the control channel 1 degrees o Gua tween frames min Here the calibration line has been clicked and dragged from one of the walls in the control channel to the other Useful calibration dimensions in CellDirector 2D A The gradient channel is 1000 um wide B The control channels are 500 um wide C The distance between the ruler marks is 500 um Also the roman numbers are 500 um in height 18 Step by Step Tutorial Frame Speed This is the time between frames within the image sequence For example enter a value of 5 ifthe images were recorded every fifth minute Note If you have chosen another step value than 1 when importing the image sequence multiply your original frame speed value with the step value If you have chosen Euclidian distance multiply the original frame speed value with the number of frames Apply and
7. Automated cell tracking of e g bright field and phase contrast microscopy images is possible using Tracking Tool PRO and the software does not require images containing fluorescently labeled cells In some image sequences it might be difficult to distinguish between cells and background Use the Background threshold slider to separate cells from background Click the Preview background removal button to view the background removal la Background threshold 0 08 Preview background removal 15 Step by Step Tutorial Preview Detection Click the Preview detection button to evaluate the cell identification parameters Make sure you have the checkbox Show contours ticked Adjust the earlier described settings and preview again if you are not satisfied with the result Autotrack When satisfied with the cell identification decide if you want tracks to start in frame 1 only or if you al low tracks to start later in your image sequence when performing automated tracking The default input will allow tracks to start in any frame Click Ignore tracks starting after frame 1 if you do not allow this after frame 1 oa fave teranlata Now click on the Autotrack button to start the automated tracking process You will first be prompted to save your project Follow the autotracking progress and the estimated time until autotracking is com plete through the bar that appears after clicking Autotrack Once Autotrack is complete a pop up win dow will ap
8. Change Settings By clicking the Apply settings button you accept the settings and values entered in the Data settings panel You need to click this button in order to continue If you thereafter wish to change the entered set tings and values in the Data settings panel click the Change settings button Track Selection Exclude Tracks The Track selection panel gives you the option to choose tracks in a specific region of interest Click the Rectangle drawing tool button left click and draw a rectangle over the region you are interested in you wish to exclude tracks of cells that have migrated short distances enter the value and click the Exclude button These tracks will not be included in the statistical analysis or plot Track selection Selected tracks 507 582 Tracks shorter than 0 0 0 RS lt a eRe AAA ra TA EN Screenshot of a selected region of interest to be used for data analysis and plots 19 Step by Step Tutorial Analysis Tab The Analysis tab will provide statistical data from the selected area of tracked cells In this tab you also have the option to export your statistics as external spreadsheet files to xls if Excel is installed on your computer other wise as a csv file Tracking Settings Analysis Plotting Merge plots My account Logout Tetai mambe ofc acs 59 Total number of cell tracks 549 Number of cells migrating 201 348 378 71 Percentage of cells migrating
9. IN GRADIENTECH 150 100 100 150 150 100 50 0 50 100 150 Tracking Tool PRO v2 0 Microscope images collected during a chemotaxis experi ment with CellDirector 2D The images are analysed using the Tracking Tool PRO software Automated cell tracking of phase contrast microscopy im ages Tracking Tool PRO does not require fluorescently labeled cells Plot exported from Tracking Tool PRO showing cell tracks from a CellDirector 2D chemotaxis experiment Introduction Save hours using the new automated Tracking Tool PRO software Tracking Tool PRO the new automated tracking tool soft ware developed by Gradientech provides users with an easy to use cell tracking and analysis software which can advantageously be used together with our CellDirector chemotaxis products Manually tracking large number of cells for long time peri ods can be very labor intense The new features in Tracking Tool PRO include automated detection and tracking of cells which drastically reduces the analysis time with main tained high quality accuracy and user control User control of the analysis quality Tracking Tool PRO is an ideal software for automated tracking of non labeled cells imaged with e g bright field or phase contrast microscopy This is due to initial state of the art noice reduction scripts making the extraction of cells in images with low contrast to noise ratio possible You
10. The length of the numerical component needs to always have the same length e g Image001 Image002 Image099 Image100 Internet Connection Tracking Tool PRO requires internet connection when using features connected to your account perform auto mated tracking edit account information order new tickets Graphics Card The automated tracking process of Tracking Tool PRO can be accelerated by the computer s graphics processing unit GPU Supported GPUs are Nvidia s graphics card from 2010 and later Make sure to update your driver at http www geforce com 5 Installation Overview User Account Log In After successfully installing Tracking Tool PRO start the program by clicking the Tracking Tool PRO v2 0 icon A pop up window will appear that will ask you to log in to your account with your username and password If you do not already have an account you can create one by clicking Create account If you have forgotten your password click on the Recover password option Username Password _ Remember me on this computer C Login skip login aa rol create account 0 recover password Note Automated tracking will only be available once you have signed in to your account Create Account To access all the new features in Tracking Tool PRO you will need assign your account with a username and password Click on Create account and enter the information that is required The username should be
11. ack Right MBlue Additional info Color of centre H Blue of mass C Show number of cells Show of cells migrating Font Arial t y Text size 100 Display paths cas Screenshot of the Plotting tab The final plot is shown on the right hand side The Axis settings Track colors and Additional info panels are shown at the left hand side The Export plot button unique for the Plotting tab is shown at the bottom within the Project management panel Axis Settings Label and Scale Axis Default values x axis um y axis um interval 200 200 min 500 0 500 0 Axis settings Label x axis Label y axis max K aXI5 600 0 y axis 6000 Track colors Label Axis To rename your axis type in the desired text and click on another text form or radio button to allow the changes to take effect 24 Step by Step Tutorial Scale Axis Change the max min and interval values for each axis to scale them If you have entered values which are too great or small and cannot find the tracks click the Default values button to go back to the original settings Your values will remain unchanged when switching tabs However if you change the data set tings in the Settings tab the plot axis scaling will now be automatically re calculated Track Colors Visualise Track Directions The Track colors panel include settings for you to colorise and differentiat
12. d tiff or as a peg file both in a format of 5729 x 5729 pixels 26 Step by Step Tutorial Merge Plots Tab In the Merge plot tab it is possible to add tracks from multiple projects and merge them into a single plot The tab includes the same settings as the Plotting tab with exception that statistical data cannot be visualised in this plot Tracking Settings Analysis Plotting Merge plots My account Logout Title and axis settings Title Title Label x axis x axis um Label y axis y axis um max min interval x axis 180 180 0 Default y axis 180 180 50 values Track colors O Allsame Left Right Up Down Left E Chartreuse s v Right 6 Additional info Endpoint size jo Font Arial y Display paths E a x P gt 2 Merge project files Files gt ES x axis um TrackingTool PRO v2 0 Merge Project files Add Projects to your Plot Create a merged plot by adding other project files gtt to the plot of your current project Click on Add then navigate to the folder where the gtt file is located Click OK You are able to add up to four gtt files In the Merge plots tab you are able to export your merged plot to an image in the same way as desribed earlier in the Plotting tab section Merge project files Files 27 Step by Step Tutorial
13. e Automated Cell Tracking 1 Create an account 2 Sign in 3 In the My account tab click on Get more tickets 4 In the Tracking tab import your image sequence tif tiff jpg jpe select frame range and click on Apply selection 5 Adjust Cell radius Cut off threshold Background threshold and click on Preview detection Repeat until satisfied 6 Click on Autotrack 7 In the Settings tab Specify the um to pixel ratio and the Time be tween frames min Click on Apply changes 8 Statistical data of the tracks can be seen in the Analysis tab 9 Stylise and export the plot in the Plotting tab 10 Quick Guide Automated Cell Tracking Step by Step Tutorial Tracking Tab Tracking Tool PRO offers you a fast and easy way to track your cells Save up to hours using automated tracking compared to manual tracking with maintained high quality accuracy and user control This section provides in structions and tips of how to get the best tracking result Get a closer look at your cells by using the zoom function Zoom by moving the slider located by the upper left corner of the frame Press the Reset button to un zoom the image to the default position Tracking Settings Analysis Plotting Merge plots My account Login Frame selection import frames Number of frames 31 Euclidian track from step to Select 01 2 1 2131 Apply selection Image settings Contrast 50 Brightness 50 Tracki
14. e between tracks of cells that have migrated Left Right or Up down O All same 8 Left Right Up Down Left H Gold v Swap Right SeaGreen Additional Info Stylise your Plot The Centre of mass COM checkbox toggles the visibility of the COM point in the plot The Endpoint size allows for changing the size where the size of 1 is as large as the cell track path lines Color of centre of mass allows for changing the color appearence of it As with the Track color panel you can simply type the first letters of the name of the color to faster access your color Clicking the Show number of cells check box toggles the visibility of the number of cells migration information Clicking the Show of cells migrating option buttons toggles the vis ibility of the percentage of cells migration information The Font drop down list allows for changing the font to one of six com mon fonts 25 Step by Step Tutorial additional mito Centre of mass Endpoint size Color of centre 7 l M Crimson of mass Show number of cells Show of cells migrating a Left Right Up Down O None Font Anal Text size 100 Display paths Centre of mass Endpoint size Color of centre of mass Show number of cells Show of cells migrating Font Export Plot Save as A High Resolution Image Click the Export plot button in the project management panel and export your stylised plot either as an uncompresse
15. ect Save a Project 000000000 Working with Tabs Workflow Overview 4 4 Quick Guide Automated Cell Tracking Step by Step Tutorial TrackingTab Frame Selection Choose your Image Sequence Image Settings Adjust Brightness and Contrast Tracking Manual Tracking Autotracking Automated Tracking Settings Tabe s ew o Data Settings Statistical Input Parameters Track Selection Exclude Tracks AnalysisTab 1 ee ee Data Explanation Export Statistical Data Excel Installed Export Statistical Data Excel Not Installed Plotting Tab Axis Settings Label and Scale Axis Track Colors Visulise Track Directions Additional Info Stylise your Plot Export Plot Save as A High Resolution Image Merge Plots Tab Merge Project Files Add Projects to your Plot Contents Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Installing Tracking Tool PRO Hardware Requirements The following computer specifications are the minimum requirements the software is designed for Platform Windows 7 and Windows 8 Minimum RAM 3 GB Minimum free space on hard drive 400 MB Minimum screen resolution 1024 x 768 pixels Prepare for Installation If n
16. he sequence where you mark the cell s endpoint To assure that the end point belong to the correct cell move the slider manually to follow the cell until its endpoint A Figure showing the difference between gt Accumulated path green line and Euclidian path dotted black line Selecting Frame Range By setting the frame range you can exclude parts of your sequence that you wish not to track From Choose the starting frame This will become frame number 1 in the frame slider control All frames before this number will be ignored To Choose the ending frame All frames after this number will be ignored Step Choose a value of skipped frames A step value of 2 for example will select every second frame between the first and last selected frame Image Settings Adjust Brightness and Contrast You can optimise the contrast and brightness of your image sequence by moving the sliders in the Im age settings panel Changing the contrast or brightness will not influence the quality of the automated tracking Image settings A Contrast 50 A gt Brightness 50 12 Step by Step Tutorial Tracking Manual Tracking Tracking a cell manually is done by pressing the Add track button followed by clicking on the cell For every click the frame slider will advance as set by the earlier described step value Selecting tracks in the track list can be made by using left mouse button together with the Shift and Ctrl keys on the
17. ickets click on Get more tickets and a pop up window will appear where you will be able to choose between 10 or 50 tickets The sales team of Gradientech will handle your order and assign your account with the requested tickets Pending ticket requests 50 tickets requested at 2014 10 23 10 51 32 7 User Account Managing Tracking Tool PRO Projects Manage Projects Tracking Tool PRO was designed to be flexible and to have an easy to use workflow To enable this Gradientech created the gtt file format containing all the information from previous work Hew Start a New Project When clicking the New project icon you will be asked to save or reject the ongoing project before starting a new project Open a Previous Project By clicking the Open project icon you will first be prompted to save the ongoing project Thereafter navigate in the Explorer window and choose which project to open Note If the image sequence has been relocated on your computer you will be asked to designate the new file path Navigate to the new folder and double click on one of the frames within the sequence Save Projects Click on the Save project icon to save your current work Be sure to save your work often to prevent lost work in case of a crashed computer 8 Managing Tracking Tool PRO Projects Working with 5 Workflow Overview The Tracking Tool PRO workflow is designed to be intuitive and the tab system sh
18. low in the x and y axis direction For example in an experiment where many cells show strong migration towards or away from a chemoattractant in the x di rection the FMIx value will converge towards 1 or 1 n FMI _ 1 Y end point 1 n dai 0 1 Xend point 1 dai Xendpoint Adcumulated distance d 4 21 Step by Step Tutorial Directness The directness of each cell is the euclidian distance divided by the accumulated distance and will have a value between 0 and 1 The directness value is the mean value for all cells Values close to 1 signals high directionality of the cells whereas values close to 0 is a signal of more random movements Directness 0 72 Euclidian distance da 1 de 1 176601655 n dai Accumulated distance d Rayleight Test p value The Rayleigh test is a statistical test for determining whether a circular distribution is random or non random The test is implemented into Tracking Tool PRO to calculate the circular distribution uniformity of the end points of the tracks The null hypothesis H of the test is that the track end points are randomly distributed The alternative hypothesis H is that the end points are distributed non randomly the cells have migrated in a uniform direction e g chemotaxis The test will return a p value between 0 and 1 With a p value smaller than p 0 05 the H is rejected is significant that the cells have migrated in a cer
19. ly A Add track same function as clicking the Add track button described above E End track same function as clicking the End track button described above D or Del Delete track same function as clicking the Delete track button desribed above N Next frame same function as left clicking when tracking a cell Ctrl Tab Switch to next tab Ctrl Shift Tab Switch to previous tab Ctrl Mouse scroll Zoom in on frame 13 Step by Step Tutorial Showing Paths and Labels Show path By checking the Show path box you will see a blue path for all the selected tracks in the track list Show labels By checking the Show labels box a label will be displayed at the start point of all tracks Autotracking Automated Tracking In the autotracking panel you can perform automated tracking of cells and save you hours of work Set the cell diameter adjust and optimise parameters to assure best quality result Single tracks created by the automated tracking feature can be removed manually You can also add tracks manually to comple ment your list of automated tracks Automated tracking is available when you have tickets on your account You can re analyse an image sequence repeatedly once you have assigned a ticket for its project file gtt Autotracking Apply template User custom z la Cell DIA 10 500 pixels la Cut off threshold 58 00 Background threshold 0 08 Preview background removal Show contours E Ig
20. ng L Show path gt Show labels Delete track End track Autotracking Apply template User custom Cell DIA 10 500 pixels a Advanced E Cut off threshold 58 00 Background threshold 0 08 Preview background removal Show contours C Ignore tracks startinc Autotrack after frame 1 Save template tel Frame slider 1 31 Frame Selection Choose your Image Sequence The first panel you will encounter in the Tracking tab is the Frame selection panel Here you choose your image sequence and which frame range to analyse Finalise your frame selection by clicking on the Ap ply selection button Frame selection importframes Number of available frames 204 E Euclidian track from step Select 7 204 Apply selection 11 Step by Step Tutorial Importing Frames This is where you choose which image sequence to analyse Click on Import frames navigate to your image sequence folder in the Explorer window By clicking on a single image file tif tiff jpeg jpg you will import the entire sequence of images You do not need to select all the images in the same sequence to have them imported Euclidan Track Definition Note Only for manual tracking If you are only interested of analysing the start and end position of the cells check the Euclidian track tickbox After clicking on a cell s starting point the slider will jump to the end of t
21. nore tracks starting Autotrack after frame 1 Save template Cell Diameter Determining the cell diameter is the first setting you will encounter in the Autotracking panel Click on the DIA from image button and select a typical cell in the image by left clicking with the mouse in the centre of the cell A green contour will appear Drag the mouse to expand contract the contour When the contour encircle the entire cell you have set the cell diameter la Cell DIA 10 500 pixels 14 Step by Step Tutorial Advanced Settings The Advanced option includes settings that are predefined Adjust these to your data set to get the most accurate automated tracking result for your particular image sequence a Advanced 7 Cut off threshold 58 00 la Background threshold 0 08 Preview background removal The Cut off threshold is a setting that includes various cell recognition criterias A higher value will induce more strict criteria hence fewer cells will be recognised by the software The predefined Cut off threshold is 58 Drag the slider then click the Preview detection button make sure to have the checkbox Show contours ticked and view the contours that appears around the cells Use the cut off threshold that iden tifles as many cells as possible without detecting to much of the background noise Cut off threshold 58 00 a ap Show contours F a a gt r S 7 F po EL 4
22. ot previously installed Microsoft s NET framework is required for the Tracking Tool PRO to run on your com puter Download the latest version of NET at http www microsoft com net Configure Installation Install the Tracking Tool PRO software by double clicking the setup msi file which will start the installation pro cess The installation requires administrator rights to initialise and may take some time to display depending on the computer system Uninstalling Tracking Tool PRO Uninstalling Tracking Tool PRO should be done through Windows Control Panel Go to Control Panel gt Programs gt Uninstall a program Select Tracking Tool PRO v2 0 in the list and click Uninstall Installing Newer Versions To be sure of avoiding conflicting software double installations first uninstall the older version of Tracking Tool PRO as desrcibed above After this is done continue with installing the newer version For MAC Users A windows emulator such as VMware Fusion or Parallels Desktop is required to run Tracking Tool PRO on Mac More info on emulators at www macwindows com 4 Installation Overview Technical Requirements Image Requirements Image format TIF TIFF JPEG JPG Minimum no of frames per sequence 5 Maximum no of frames per sequence Unlimited Color mode Greyscale 8 16 bit Image sequence notation The image sequence should contain a text string including a numerical sequence in ascending order
23. ould be used in an left to right manner However you can always return to a previous tab if you wish to correct or complete previous work Tracking Settings Analysis Plotting Merge plots My account Tracking tab This is your startup tab Here the image sequence will be import ed and handled This is also the section where all the detection and tracking will be done Settings tab Here you assign the data set parameters so that you get accurate statistical information and plots in later tabs Here you get the option to rotate your images to correct for unintended rotation during the experimental setup You will also be prompted to provide information such as frame speed and um to pixel ratio of your images which is important for the statistical analysis Analysis tab The analysis tab will present useful statitics from the selected ar eas of tracked cells In this tab you also have the option to export your statistics as external spreadsheet files to xls if Excel is installed on your computer other wise as a csv file Plotting tab Here you will be given the option to stylize and export the plot containing the generated tracks Merge Plots In this tab you are able to add other project files gtt to your plot Tracks from all added projects will be seen in one single plot My Account Here you are able to edit your account information order tickets and view your ticket purchase log 9 Working with Tabs Quick Guid
24. pear with information about the tracks that has been created Click Continue to access the statistical data of generated tracks Automated cell tracking completed successfully with 629 tracks created Average tracking length 20 89 frames Average start frame 4 54 Average end frame 25 43 249 tracks starting after frame 1 380 tracks starting in frame 1 Press continue to access statistical data and plot your tracked cells A ticket will be withdrawn if one has not already been assigned to this project A ticket will be withdrawn and assigned to this image dataset Note A ticket will be withdrawn from your account first when you have approved Once a ticket has been asigned to your project file gtt you will be able to autotrack it as many times as you desire Save and Apply Templates Note This is a powerful time saving feature ideal for comparable imaging sequences generated from equal experimental conditions where the same settings can be applied and analysed thereafter Save your settings by clicking on the Save template button Save your template file in the default Tem plate folder Use a saved template for another data set by selecting the approriate template in the Apply template drop down list 16 Step by Step Tutorial Settings Tab In the Settings tab values will be set for Tracking Tool PRO to calculate precise statistical data and to export ac curate
25. tain direction Note A larger number of tracked cells give a more significant p value Cells usually migrate with the flow direction Use the Rayleight test p value for comparison between regions gradient or control channels or experiment Rayleigh test p value 2e 004 22 Step by Step Tutorial N l Fischer 1993 Statistical analysis if circular data Cambridge university Press Export Statistical Data Excel Installed Click the Export to spreadsheet button to export your analysis data as an xls file if Excel is installed on your computer Export Statistical Data Excel not Installed If Excel is not installed on the computer you can still export your data as a spreadsheet file The data will be exported as a csv file 23 Step by Step Tutorial Plotting Tab In the Plotting tab the style and information of your plot can be set and high resolution images can be export Number of cells 549 Left 37 Right 63 E a x 1 gt TrackingTool PRO v2 0 ed as tiff or peg files Tracki Settings Ana 15 Plotting Me lots My account ng ng 9 rge y x axis 1800 1800 50 lnea y axis 180 0 180 0 so values Allsame Left Right Up Down C Centre of mass Endpoint size 10 v O None Left Right Up Down Title and axis settings Title Title Label x axis x axis um Label y axis y axis um max min interval Track colors Left a Bl
26. will experience excellent automated tracking results of your cells even if the cells are clustered appear or behave differently The automated tracking module creates tracks by statistical means where contours and distances are im portant factors Results can be checked and changed manually after the au tomated processing to assure best quality possible Easy and affordable automated tracking Tracking Tool PRO is a ticket based analysis software which offers you an easy and affordable way to perform automated live cell tracking The user friendly interface help you assign parameters detect and track cells auto matically get statistics and export final plots all within a stand alone software Installing Tracking Tool PRO Hardware Requirements Prepare for Installation Configure Installation Uninstalling Gradientech Tracking Tool Installing Newer Versions For MAC Users 1 ee ee eee Technical Requirements 4 Image Requirements Internet Connection Graphics Card 8004 User Account e sso ooo oo LOGI s 2 e NE Create Account 000000 My Account 0000 Edit Account 222 2 4 eee Ticket System oaa a ee Managing Tracking Tool PRO Projects Manage Projects 4 Start a New Project Open a Previous Proj
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