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MIT-30-1

1. Tog displaying on the second line on LCD it stands for system enter end of work time programming setting method is same as start of work programming After operation press c again to return working mode and lock start end setting time E Note Please make LCD go back to initial contition mode after setting 5 5 6 Programming defrost definite time This is defrost definite time button Under initial mode after pressing first line on the LCD displays Defrost Second line displays Time User may press H and M buttons to adjust hour and minute If it is needless please adjust hours to 24 Press button again to go back initial mode and lock defrost definite time 5 6 Usage of small operation panel Fig 4 small operation panel H 5 6 1 UV sterilize This is UV sterilize button Press it to activate UV lamp and Indicator light is on UV lamp in the freezing chamber is on and begins to sterilize It is automatic timing after activating and it will self close in 35 minutes User may press button once more to stop it at any time Indicator will be off correspondingly Note Keep quartz tube cleaning is the key point for sterilizing We j suggest user to regularly clean it to maintain sterilizing ability N Because UV sterilizing is unfit for low temperature It is better to start to sterilize under stand by situation mn Note When doing UV sterilize please close glass window and do not let UV lig
2. Right area Fig 1 5 2 Turning the Instrument On First connect power line on the rear of cryostat then turn on switch on the rear of cryostat Cryostat is under working condition and feeding arm go back original position Compressor starts to work after turning on power switch and freezing chamber begins to cool down It depend the interval of turning off and on if within 3 minutes the instrument needs to wait another 3 minutes otherwise machine easy to damage Two red LED lights above right area first one is for big compressor and second is small compress When compressor starts to work the first light is on If press fast freezing button second light is on Control board is separated into three area left area middle area and right area Left area is for software switch and fast defrost buttons middle area is for section trimming setting peltier and stand by function control right area is for time and temperature setting Small control panel on the left of cryostat controls clamp feeding illumination and UV sterilizing 5 3 Left area control buttons introduction 5 3 1 Software switch Press this button to switch on or off operation panel If indicator is on user may adjust time defrost temperature and so on Otherwise indicator is off and panel locked User cannot do any operation 5 3 2 Immediate defrost se o f This is fast defrost button Press it to activate fast defrost function and indicator light is on oy
3. in the selected location can be tight Slice half way when necessary change blade only need to release clamping lever for locking blade then replace the blade 6 4 Adjust the anti rolling plate Blade Parallel Knob for clamping anti rolling plate Using hexagonal wrench to make Anti rolling plate height turning knob pia J parallel between blade and slide Fig 7 Making parallel between edge of blade and Anti rolling plate The anti rolling plate is made of Plexiglas Adjustment includes 1 Make the edge of the anti rolling plate parallel to the edge of the blade Insert blade and lock the clamping lever Lock two knobs Using hexagonal wrench to make parallel between blade and anti rolling plate Fastening hexagonal screw Turing height turning knob to adjust height of anti rolling plate 2 Adjust the anti rolling plate angle with the blade Fig 8 Anti rolling plate a 7 Missie _ Adjustable angle Angle of cutting blade edge Fig 8 A The angle shall be no more than 10 3 Make the edge of plate and edge of blade meet flush Fig 9 Edge of plate and blade meeting flush Anti rolling plate height turning knob Fig 9 Making flush between edge of blade and anti rolling plate To protect the upper part of the anti rolling plate connecting with tissue spec
4. state right LCD on control panel is showing information and data 16 18 as Fig 3 We call it initial mode when cryostat get power supply 16 off and reset First line Day is to present current operational day Fig 3 numbers from 1 to 7 separately stand for Monday to Sunday Second line Time is to present current operational time Third line TO is to present chamber temperature It is actual temperature value for operational room following value in is setting temperature of freezing chamber Forth line T1 is freezing shelf temperature It is actual temperature value for freezing shelf Off in is to stand for peltier off User can press D to adjust working day Then press hour button H and minute button M to adjust hour and minute respectively This button is to select temperature Press button per time the third line on the LCD will shift between TO Chamber temperature and T2 Clamp temperature 5 5 2 Temperature setting a Press this button to increase temperature value Value in in the third line on LCD will add on If LCD shows TO it is to adjust freezing chamber setting otherwise it shows T2 to adjust clamp temperature This is decreasing button Function is same as above 5 5 3 Fast freezing It is invalid under standby mode This cryostat has double compressors Big compressor freezes for chamber and shelf Small compressor does not work if system is in stand by when system goes back to
5. system goes into fast defrost mode It takes about 6 minutes to defrost Press Ie button again to stop fast defrost working indicator light goes out simultaneously Freezing recovers after 25 seconds If system automatically enter time defrost mode indicator lights up too Press button again to stop defrost and indicator light goes out 5 4 Middle area control buttons introduction 5 4 1 Peltier freezing It is invalid under stand by mode This is peltier switch button Press it indicator light is on and peltier element begins to work and cools down the stations Forth line on the screen shows 015 to indicate peltier working 15 minutes The countdown of remaining cooling time is displayed such as 014 013 002 001 until peltier stops freezing and indicator off During Peltier working time User may press this button to stop peltier when it is activated In addition if freezing shelf temperature is higher than 10 C when peltier working peltier will self close On the same principle if freezing shelf temperature is higher than 10 C peltier cannot be activated 5 4 2 Standby We call the instrument into Standby mode by keeping the freezing chamber at a temperature of 4 C to 9 C This time the operational time of the compressor is less than the stop time with average power consumption decreased more prolongs the lifetime of the compressor When long time do not use the instrument for slicing t
6. the cutting edge facing upwards Before changing specimens always lock the hand wheel In case of malfunction contact our company Don t try to solve it by your own risk 1 Introduction Model MIT 30 1 Semi automatic Cryostat Microtome national regulated ist class medical equipment is a rapid pathological section instrument to human and animal body tissues It can be widely used for pathological diagnosis analysis and research in hospitals medical colleges legal medical experts and propagation institutes The instrument mainly consists of 4 parts 1 The computer controlled panel is on the upper part to display the temperature and the working 3 4 condition of the instrument The center part is the constant low temperature room to rapidly freeze the vivid tissue and to conduct the cutting area Behind is the mechanical transmission and motor drive The lower part is the compressor unit for cooling 2 Scope of Application Pathological section and study histology of plant animal or human being 3 Technical Parameter Section thickness range 1 80um Adjustable 1 20um Increment 1pm 20 40um Increment 2um 40 80um Increment 5um Trimming thickness range 10 400um Adjustable 10 50um Increment 5um 5 100um Increment 10um 100 400um Increment 50um Specimen retraction 0 95um Adjustable Increment 5um Horizontal stroke 20mm Vertical stroke 60mm Voltage AC 220V422V Frequency 50Hz Powe
7. Laboratory Equipment Manufacturer A Ww www mrclab com CE Net Operation Manual for Cryostat MIT 30 1_ PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3 Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC VER 01 3 12 Helpful Suggestions User manual is a part of the instrument The user should read this user manual carefully before first installation and operating the machine and put this manual beside the instrument to refer it when meet problems MIT 30 1 must be operated by user who is trained Before operation please carefully read operation instruction first to know operation steps and process Unit must ship upright If this unit was shipped lying down or stored lying down then it MUST stand upright for 36 hours before use Do not put instrument under extreme temperature and high air humidity environment Failure to follow this will cause instrument severe damage Do not place instrument directly under sunshine or outlets of air condition No other obstruct around the instrument in 30cm distance Check your power socket is well grounded Ensure that power supply is stable constant and adequate Compressor requires a start up current between45 and 50A Therefore the professional electrical engineer must inspect the electric circuit before installation to meet smooth operation of the instrument requirement Blade is sharp beware when you change it Do not place blade anywhere with
8. e holder forward and GE a Clamping lever for locking blade Turning lever to move blade holder left to right Turn rotator to adjust Slide hegit wramg ob z bi cutting angle of blade Fig 5 Blade holder introduction Microscope Slide Handle for replacing slide ear L L H L W H fj j f p S L 1 L TL D zga NA a Clamping lever for moving blade holder fro and back Fig 6 Blade holder introduction Release anti clockwise clamping lever on the left manually move the holder fro and back and lock it at the desired position When the holder moves to the terminal point the whole blade holder can be taken out and put it back by reverse procedures Release anti clockwise lever on the right manually move the holder left and right and lock it at the desired position A Note Remember to lock well the blade holder to right position when doing forward or backward specimen adjustment Release clamping lever on the upper right to open blade clearance insert blade from left to right then lock well the clamp lever mn Note Blade is sharp beware when you change it 6 3 Adjust the angle of the blade Use hexagon spanner to loose the rotator Knife folder can be turned and
9. h to clean the dust on the projecting vane of condenser or use high pressure air to blow it 8 6 Frequently clean the constant temperature chamber to keep it clean Pay special attention to not hurt by the blade 9 Cleaning 9 1 Cleaning up the instruments 9 1 1 Conduct the following steps before cleaning each time Turn up the specimen grip to the top and lock the hand wheel Release the specimen grip and pull it out Pick off the blade from the blade holder and put it back to the blade box Dismount the blade holder and its seat to clean up Take down the specimen from the specimen nip Clear away the section waste with dry brush Take down the specimen grip to clear up separately 9 1 2 Instruments and external surface If necessary the external painted surface can be cleaned with light duty commercial housework cleaner or suds And then use wet cloth rub it until dry You may use the substitute of xylene grease oil grease scavenger to erase residual The instruments must be dry when use again 9 1 3 Blade holder Please according to following steps to clean up the blade holder if it had been dismounted Turn the eccentric rod handle in the lateral of the body of revolution and draw it out from sideward Push the knife clamp back which have knife clip and shift it out from the rotary unit Turn the eccentric rod handle in the lateral of the knife clap and draw it out from sideward Dismount the knife clamp Clean up a
10. hen let it go into Standby mode z This is standby button Press it one time system goes into standby mode or transfer to work state LCD screen background light tells us how to differentiate from standby and normal working If background light is in dark it is standby mode On the contrast it is working 5 4 3 After switch on cryostat the first LCD will display data as Fig 2 First line Sect is to represent section and its value second line Retr is to represent retraction and its value third line No is to represent total pieces and its value forth line Tn is to represent total thickness and its value Fig 2 m This is mode selection button Press it may select Section Sect or Trimming Tim working mode C is clear button Push it to clear total pieces and thickness into 0 In addition these two buttons are combination keys If user push them together there is one signal appearing in front of Rect on the second line of LCD to show it under adjustable condition User may press or to change retraction value button is to increase value Push it to add on section trimming value If signal appearing in front of Rect it is to adjust retraction value button is to decrease value Function is same as above 5 5 Right area control buttons introduction 5 5 1 Clock adjust After cryostat switching on it is self running under current time 1 12 00
11. ht goes out of chamber 5 7 Lamp a O This button is on and off button First push it the lamp is on second push it the lamp is off Indicator 5 7 3 Slow Feeding Specimen position is adjustable by moving forward or backward Press button specimen is moving to user press t button reverse moving 5 7 4 Fast feeding Press double arrow buttons to fast move clamp forward or backward When user press backward button clamp will go back to backward limitation if need to stop on the midway please lightly touch forward double arrow button then clamp will stop moving Alarm will warn while specimen is moving to front or end limitation Note When adjust specimen forward or backwards movements the hand wheel must lock at the right position Fig 7 5 14 Use of the wasted liquid barrel Use the wasted liquid barrel to collect defrost liquid tissue thawing solution flushing liquid and wasted objects In order to prevent the human and environment hazard from the wastes put 200ml 10 formalin or other sterile solution in the barrel in advance The disposal of the wastes shall follow the related regulations of the hospital Disconnect the tube that link with the freezing chamber then take off the barrel yn Note Use the right and effective method to treat the wastes 6 Mechanical introduction 6 1 Blade holder position adjustment Fastening slide knob Clamping lever for moving Oo blad
12. imen without any damage When the quality of the tissue section is not good Should view the cutting edge anti coil whether organized debris grease and other foreign matter 3 Avoid to touching the front of the plate because high temperature will stick the tissue section 6 5 Slice Reference 1 When use the cryostat microtome better to control the blade speed and correctly adjust the anti rolling plate is an important factor to cut out high quality sections The proper speed of the blade can only be acquired by practice experience and the skillful control of the hand wheel The adjust position of the anti rolling plate is more Sometimes they interfere with each other it needs careful adjustment without random putting them on the blade edge 2 When freeze the living tissue the water content in it will condensed into ice the tissue will be hard The hardness of it changes with the temperature the lower temperature changes the harder the tissue becomes Different tissue under different temperature to have high quality sections this skill can only be got through practice Most cutting temperature of the specimen tissue without fat and formalin better to select between 13 C 23 C 3 In order to obtain high quality pieces of tissue pay attention to the following 1 Proper selection of working temperature in the chamber 2 Correct slicing operation 3 Fine adjustment of the anti rolling plate 4 Sharp slicing knife correc
13. ing Clean the blade with hair brush The clearance angle between the blade and the anti rolling plate is too narrow Vertical crack of tissue section Edge of the blade has defection need grinding Foreign matter on the blade Tissue section narrowed Adjust the clearance angle of anti rolling plate and the blade evenly Tissue section broken Too low temperature of the tissue too long time freezing the tissue is affected Vibration when slicing Unfixed blade The angle of the blade is too big or too small The specimen disc is not fixed well Cutting speed is too fast Dull blade Uneven thickness of the tissue section Angle is too small The specimen disc is not fixed well 8 Safety and Maintenance 8 1 Make hand wheel in the lock position when the instrument stops 8 2 Open the glass door after the instrument stops to let the steam vaporized and keep the working room dry 8 3 Change the broken light accordingly 220 V 15W pay attention to safety when doing the exchange 8 4 The elements of the electric system such as control panel electric control box only use expert for the maintenance others no touch 8 5 The cooling system mainly consists of compressor condenser filter and evaporator The condenser with fan may accumulate dust after long time usage which can affect the cooling function It is necessary to open the side ventilation board and use brus
14. ll parts of the blade holder Don t use xylene or alcoholic liquid e g glass cleaner when clean up Make the blade holder dry and assemble it together Apply to thin layer of lubrication after clean up the parts which had been taken off When fix the knife clip make sure that its upper part is parallel with the back edge of the knife clamp seat 9 2 Lubricate instruments Do oil lubrication for the following parts monthly 1 2 drop is well enough Instruments and specimen holder Grip draw in the clamp Lock in iron at the T knife clap back of the microtome bedplate The blade holder slide way on the microtome bedplate Blade holder Lock in iron at the T body of the rotary units on the knife clamp seat The knife control grip is shift to the eccentric rod handle The iron locking head on the knife clamp of the T body of rotary unit and the knife holder with slide way The grasping joystick of the blade Main control panel wasted liquid barrel RS Fig8 Cryostat overview Moving handle Handwheel 15 MIT 30 1 Semi automatic Cryostat Microtome Standard Setting List No Item Quantity 1 Main Machine 1 Set 2 Specimen Disc 10 Pieces 3 Disposables blade 1Box 50 blades 4 Fuse Tube 20A 2 Pieces 5 Fuse Tube 1A 2 Pieces 6 Appliance Stand 1 Piece 7 Power Supply Line 1 Line 8 Hexagonal Wrench 1 Set 9 Rubbe
15. normal working from stand by fast freezing is activated Small compressor is going to start in 3 minutes and freeze clamp Operator may cancel fast freezing regarding their own situation Press fast freezing button button green indicator is on and with sound of compressor initializing At same time red LED on the panel for small compressor indicator should be on Clamp temperature gradually decrease with working of small compressor Press this button again two indicators are off and small compressor stop working 5 5 4 Programming start of work time If second line on the LCD is in Time mode Press button until second line shows Ton Then user can press D to adjust working day Press D button one time working day will minors one day Then press hour button H and minute button M to adjust hour and minute respectively Definite time ivterval is 10 munites automatic defrost is same User can press W DAY button to set single day start of work time too After push W DAY button first line in the LCD should change to Dwork 1 amp 6 1 amp 6 represents Monday to 5 Saturday Press D button to select working day then press H and M buttons to adjust hour and minute respectively System automaticlly set 24 hours for nonworking day and goes into standby mode to reduce electricity consumption 5 5 5 Programming end of work time Press c button as many time as
16. r 850W Largest start up current 5 second 45A Chamber temperature 10 C 30 C Adjustable Freezing shelf temperature lt 40 C Number of freezing station 10 Pelletier number 1 Refrigerant R404a 300 10g Compressor oil 0 6L EMKARATE RL 22S ICI 4 Working Condition and Pre adjustment 4 1 The instrument is movable floor type with 4 caster wheels Two front wheels can be up and down adjusted by turning knobs on the foot These front wheels only function when the instrument needs to move After remove the package put the front wheels on the ground and move it to the working position and secure it then disable the front wheels 4 2 The instrument can only be used after 4 hours standing still 4 3 Confirm the power supply is grounded triple socket then connect the instrument with the main power socket turn on the power switch at the back of the instrument the machine is in operation mode 4 4 Other materials are not allowed to put on both sides of the instrument At least to leave a 300mm space to facilitate ventilation and heat dispersion 4 5 The instrument working ambient temperature is 5 C 28 C If the temperature is above 26 C air conditioning must be installed otherwise the life time of the cooling compressor will be shortened 4 6 Environment relative humidity is no more than 80 A High temperature or humidity will influence freezing effect 5 Operation 5 1 Operation panel Fig 1 Left area Middle area
17. r Sleeve 2 Pieces 10 Brush 1 Piece 11 Handle 1 Piece
18. t cut angle 4 The freezing section is just opposite to paraffin slicing the freezing section is not cut the long edge better to cut the shorter edge which means the line of contact is shorter 6 6 Handwheel locking To give operator more convenience and safety we increase locking position from 3 to 8 The first locking position is at the top while gearing handle is facing to operator The other seven positions are showing in the Fig 10 Locking spanner Rotary lever 7 Troubleshooting Trouble Solving Method Reason Tissue crack Select thinner tissue The freezing time is not quick enough Tissue specimen off Apply more embedding material The cutting tissue is soft The temperature of the specimen disc is low Tissue moves on but without slicing Ensure the fastening of the blade Fix the specimen clamp Ensure the fastening of the specimen disc on the clamp Increase the blade angle Tissue section rolling Check the gap between the anti rolling plate and the blade Increase the height of the anti rolling plate Check the angle of the anti rolling plate and the blade Tissue becomes soft when cutting Prolong the refrigeration time of the anti rolling plate and the blade Tissue section stick to the anti rolling plate Use short hair brush to clean the anti rolling plate Prolong the refrigeration time Tissue section overlapp

MIT-30-1

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