User`s Manual
Contents
1. Cancel After log set up is complete select Enable Datalog A prompt will appear asking if user is ready to enable logging select Yes Note If data logging is set to start immediately there will be a minimum delay of 100 seconds before startup after power has been supplied 998 2205 Rev E Page 21 Multi Sensor Platform 3 2 4 Downloading and File Management Users can download new and exisiting files saved in the C6 Multi Sensor Platform 4 Select Download Data Note This step may take several minutes depending on file size Choose the files to be saved by clicking the Save box Users will be promted to chose a folder location where data will be saved Files will be saved in a CSV format C Soft Manager File Settings COM Port Settings CurrentData LogSetup Download Data Used Data Log 0 Select Clear C6 Datalog to erase existing data from memory Download Data Wy A d Ov Help I 1 1 60 70 80 Clear Datalog Select files to save Connected e C Soft Manager xj Start Date Time Start Date Time Start Date Time Start Date Time Start Date Time Start Date Time Start Date Time End Date Time End Date Time End Date Time End Date Time End Date Time End Date Time End Date Time Total Files 0 Records Save 2 0 B Records Save 0 a Records Save O Records Save 0 a Records Save 0 Records Save 0 a2. Records Save 0 xi Users are able to upload data and view in the Current Data window if desired 3 2 5 Enabling Data Output Ready State 998 2205 Rev E Users interested in integrating the C6 Multi Sensor Platform into CTDs or Third Party Platforms must enable the C6 s Data Output Ready State using C Soft Software There are five necessary steps to ensure the instrument is properly setup for data output Page 22 Multi Sensor Platform 1 Set the instrument s clock to PC or local time in the Settings Tab 2 Select the sensors you expect to use during sampling 3 Calibrate selected sensors for either Direct Concentration Mode or Raw Fluorescence Units Blanked RFUB Mode see section 3 2 2 4 Set the start time sampling and wiping intervals in the Log Setup Tab Note wiping interval is optional and depends on the sampling interval see section 3 2 3 5 Click the Enable Datalog button followed by the Yes button to enable the Data Output Ready State A few seconds after the Yes button has been clicked to enable the Data Output Ready State the C Soft program will automatically shut down and users must disconnect power and serial connections The instrument is now in the Data Output Ready State and will remain in that state so long as C Soft is not used to communicate with the instrument Note As soon as C Soft is used to communicate with the instrument users will need to re set the parameter settin
3. blank subtracted or scaled to a standard they are relative values 998 2205 Rev E Page 16 Multi Sensor Platform 3 2 2 1 Direct Concentration Calibration Following steps 1 5 will ensure values are reported as Blank subtracted Concentrations in units of choice See Appendix B for recommended practices Step 1 Blanking Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a blank solution Note A blank solution is a solution without the fluorophore of interest i e de ionized water artificial seawater or filtered seawater Note Ensure the Cyclops 7 sensors are free of bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Future measurements for this sensor will be blank subtracted based on the blank response recorded during calibration Step 2 Standard Value and Units Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a standard solution or use a secondary solid standard see Appendix D for more information on solid standards Note Not all sensors are designed with solid standards See the Cyclops 7 manual for available secondary solid standards 4 Enter the known concentration value of the standard solution in the Standard Value box If the concentration is not known enter an arbitrary value i e 100 Future measurements will be calculated based on the correlated response to the standard value Enter manuall
4. 2205 Rev E Page 26 Ge Platform 5 0 Maintenance and Warranty 5 1 Rinsing The C6 Multi Sensor Platform should be rinsed or soaked in freshwater following each deployment 5 2 Care for the bulkhead connector Install the 8 pin female end plug to the bulkhead between uses A light coat of Silicone based grease should be used on the rubber of the male pins of the bulkhead to aid in sealing 5 3 Care for the sensor ports Install 6 pin male plugs into any vacant port A light coat of Silicone based grease should be used on the rubber of the 6 pin port end to aid in sealing 5 4 Terms Turner Designs warrants the C6 and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for accidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Da
5. 6 16 08 10 34 58 69 72 76 64 0 00 21 47 06 16 08 10 34 59 70 24 172 20 0 00 21 47 Chan 1 Rhodamine 80 44 _ Calibrate 06 16 08 10 35 00 70 40 174 72 0 00 21 48 06 16 08 10 35 01 68 92 74 28 0 00 21 48 Chan 2 Chlorophyll a o Calibrate 06 16 08 10 35 02 67 92 78 28 0 00 21 47 06 16 08 10 35 03 71 28 73 20 0 00 21 47 Chan3 Phycocyanin 76 8 Calibrate 06 16 08 10 35 04 71 76 77 04 0 00 21 48 06 16 08 10 35 05 73 88 77 00 0 00 21 47 Chan 4 s NE 06 16 08 10 35 06 73 24 76 24 0 00 21 47 A 06 16 08 10 35 07 72 48 74 00 0 00 21 47 CAR 06 16 08 10 35 08 73 76 73 28 0 00 21 47 Calibrate 06 16 08 10 35 09 74 48 79 60 0 00 21 48 06 16 08 10 35 10 76 28 76 64 0 00 21 47 Calibrate 06 16 08 10 35 11 75 68 75 92 0 00 21 47 06 16 08 10 35 12 72 12 72 36 0 00 21 48 Depth meters 0 Calibrate 06 16 08 10 35 13 73 60 74 40 0 00 21 47 06 16 08 10 35 14 72 64 172 64 0 00 21 48 Temp c E 06 16 08 10 35 15 75 40 71 24 0 00 21 47 06 16 08 10 35 16 74 00 70 80 0 00 21 48 06 16 08 10 35 17 76 44 73 20 0 00 21 48 06 16 08 10 35 18 74 64 78 16 0 00 21 48 06 16 08 10 35 19 76 32 74 24 0 00 21 48 06 16 08 10 35 20 78 08 75 12 0 00 21 48 Gun aa nae 06 16 08 10 35 21 79 04 72 28 0 00 21 48 06 16 08 10 35 22 81 48 74 76 0 00 21 48 06 16 08 10 35 23 70 84 78 52 0 00 21 48 06 16 08 10 35 24 76 16
6. 74 60 0 00 21 48 06 16 08 10 35 25 81 36 73 28 0 00 21 48 Start Data Capture 06 16 08 10 35 26 80 52 72 92 0 00 21 48 06 16 08 10 35 27 80 56 75 68 0 00 121 48 06 16 08 10 35 28 78 60 78 36 0 00 21 48 06 16 08 10 35 29 80 44 76 80 0 00 21 48 x E 998 2205 Rev E Page 14 Multi Sensor Platform Waveform Screen 4 Ss e Users can graphically view real time data for each sensor by clicking the Display Waveform box Sensor identification name and units will be displayed on the Y axis Time and date will be displayed on the X axis Graphs can be autoscaled by clicking the Autoscale box Data can be saved during waveform mode by clicking Start Data Capture C Solt Manager IE Fit Sabinas COM Port Hala Data Log Chart Autoscale w Settings Current Data LogSetup Download Data o luna bos E j fi A T AAAA A de a j Ny yy le Vil A A ho Ta Curent Data pa laci n Sl PEA IA 7 i Chan 1 Chlorophwyll a 15 52 Calibrabe i Chan 2 Brighteners 14 23 Calibrate ae E Chan 3 Fluorescein 35 76 Calibrate oc Chan 4 CDOM 12 8 Calibrate g Chan 5 Phycocyanin 65 64 Calibrate Chan 6 Ol 23 6 Calibrabe Depth meters Calibrate Temp C 23 1 View Cabbration Dita Start Date Captura EE 6j29 2007 4 el Lal 998 2205 Rev E Page 15 Multi Sensor Platform Calibration Screen Users can calibrate Cyclops 7 sensors in either the direct conc
7. 998 2205 Rev E Page 11 Multi Sensor Platform 3 1 2 Software Installation 1 Exit all Windows programs 2 Insert the C Soft CD also located on the Turner Designs Software webpage http www turnerdesigns com t2 sw main html Open the C Soft software folder and double click on the setup icon 4 Double click on the setup exe icon to begin installation 5 The software will automatically be installed in the PC s C Program Files C6 folder unless user selects alternate location 6 After restarting the PC double click on the C Soft icon located on the desktop Gott C Sort Note You may receive a warning message asking if you want to unblock messages select Unblock 3 1 3 PC Software Interface 1 Connect the 9 pin serial end of the interface cable into the serial port on the back of the PC Note If your computer only has USB ports we recommend purchasing a USB to serial adapter cable See Turner Designs Support webpage for recommendations 2 Connect the 8 pin female end of the interface cable to the 8 pin male connector on the C6 Multi Sensor Platform Connect the interface cable s 12V pin to the 12V power supply Initializing Unit 4 Plug the 12V power supply into a power source Progress Wait for initialization process to complete a 6 The connection icon located on the upper right of the screen will turn green if connected C Soft Manager Connected J File Settings C
8. Dark Black Non Reflective Surface 998 2205 Rev E Page 31 Platform Appendix C C6 Multi Sensor Platform Wiring Guide The C6 Multi Sensor Platform outputs digital data in a format that can be read by C Soft software or a terminal emulator program It can be integrated into larger multi parameter systems such as CTDs or buoys An 8 pin impulse cable provides two end connections 1 a 9 pin RS232 serial port for connection to a PC or laptop computer and 2 a 12V port for supplying power to the unit C6 Multi Sensor Platform bulkhead and serial port connectors DVO 009 Sub D Connector Wire Guide Pin l Out Color C6 Function Corresponding Sub D Connector Pin 1 Black VBATT 4 OW a a a DiR O OPNS 4 Green RX gt gt PN O 5 Bue TX _ PINS O MOE ORE O E L Yellow RTS amp CTS PIN 7 8 wes N A N A 998 2205 Rev E Page 32 G Platform Appendix D Using the Secondary Standard The following information will describe how to use the Solid Secondary Standard P N 2100 900 with Cyclops 7 sensors Note The Turbidity Cyclops does not have a solid secondary standard Solid Secondary Standard Benefits 1 It can be used in place of a primary liquid standard once a correlation between a primary standard and the solid standard has been established 2 It can be used to check the stability of the instrument and or check for loss in sensitivity resulting from the growth of bio fouling orga
9. E Page 34 Multi Sensor Platform Linear Range and Quenching The linear range 1s the concentration range in which the Cyclops 7 output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship 1s seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is known as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Fluorometer Response Curve Graph showing Linear and Quenching Regions of the sample s response __Linear Region Sample Concen Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature Fo
10. OM Pork Window Help Note f the connection icon does not turn green disconnect and reconnect power 998 2205 Rev E Page 12 Multi Sensor Platform 3 2 Software Operation 3 2 1 Settings Screen The Settings Screen allows users to set the time and date identify each Cyclops 7 update serial numbers and select sensors to be enabled Users can manually set the date and time or choose to synchronize the C6 Multi Sensor Platform to the PC date and time Each channel 1 6 in the Settings Screen has a port associated with it on the C6 Multi Sensor Platform that is numerically labeled 1 6 respectively Click on the box adjacent to the channel to enable channel operation If there is no sensor installed users will be unable to select that channel Channels can be renamed using the dropdown selections or by manually entering a name Channels can also be named using Cyclops 7 serial numbers Settings will automatically be saved after exiting the Settings screen C Soft Manager File Settings COMPort Window Help Connected Q Settings Current Data Log Setup Download Data C Soft Manager xl Channel Settings Enable Name Serial Chani M Rhodamine Unit Current Time Chan 2 10 32 20 Retrieve Unik Time Chlorophyll a 6 16 2008 Chan3 Mo phycocyanin Chan 4 Current Time 10 32 27 Set Unit To PC Date amp Time Chan 5 6116 2008 Chan 6 Depth 4 Manually Set Unit Date amp Time Temp Cancel Set
11. Platform and can be locked into place limiting movement during transportation deployment and retrieval 1 Place the fastener on the center port of the C6 Multi Sensor Platform and rotate to tighten Make sure not to over tighten 2 Align the 6 pin male end of the mechanical wiper with the C6 Multi Sensor Platform center port and fastener 3 Hold the mechanical wiper in place and rotate the remaining ring to tighten This will lock the wiper into position 4 Open the C Soft software See section 3 0 998 2205 Rev E Page 9 Platform In the Log Setup screen click the Test Wiper button to home the wiper motor The mechanical wiper will make one revolution setting the wipers motor shaft to its home position Motor shaft Fastener x Wiper nut Brush PP v 5 Slide the wiper brush assembly over the wiper motor shaft 6 Position the wiper brush between the Cyclops 7 optical heads 7 Hand tighten the wiper nut to lock the brush assembly into position 8 Click the Test Wiper button to ensure wiper will home to that position Note Install a 6 pin waterproof plug into the center port when wiper is not installed 2 2 3 Installing the Flow Cap Overview The C6 Multi Sensor Flow Cap was designed to enable flow through mode and can be configured with other instruments with online flow through systems The flow cap eliminates the possibility of ambient light interference and it can be used as a calib
12. ST 13 322 Current Data and Calbraton orecl ada 14 Current Data ro oia io 14 Tabular Srei a das 14 VV Aveo FM Mi id ia olas 15 Calbration Screen a a OD A 16 32 2 Direct Concentration Calado cl isc 17 3 2 2 2 Raw Fluorescence Calibration Blank Subtracted ccccccconnooooooooconnnnnnnnnnnnnnnnnononnnnananennnnss 18 za Fluorescence Cabra tlacta ous thabictictanatseubag a A 19 2 DEPE ADON s a a a A 19 Atmospheric OLS t iia A A A tense euiaiaees 19 Atmospheric and Gravity Offset oooocooooooooncccnnnnnnnnnnonocccnnnnnnnos 19 NS A eerie st wk ania tds sak E T E TO E Saou E ETE 20 3 24 Downloading and Fle Ma as MEM AA Seeley 22 3 25 Enabling Data Output Ready Stale a ad 22 Integration Cable for Digital ASCII Output 0 00000 eee 23 3 2 6 How to setup Hyperterminal for Digital ASCII Data Capture ooooooonnnnncnnnnnnnnnnnnnnnnnnnonannnnnnnnos 24 TASA A A A 26 4 1 Connecting the Submersible Battery Pack co did 26 4 2 Battery brackets tala did 26 SO MVIGUILCNGNCE and WATIN nist aie evi ise oie S 27 Jle A a S a aentdancuaat dana asa Mlabnlsae tances 27 3 2 Care for the bulkhead COnneCtOr caia E N E E 2 5 3 Care for the Sensor PONS a id 27 A passe E EESE AE IEEE EEN EE ET SE EE EEE R PE AEE EEE E EET POTETE 27 Multi Sensor Platform See de LE OLA SEL LO O IA PO ERE E o A teas tectes 2q MO OU W Alla ty S EIC E T muaaenouaseontenest 28 APPS PEC OS os eats a e eat 30 Appendix B C6 Multi Sensor Platfo
13. Sensor Platform comes with factory installed temperature and pressure sensors and is rated to a depth of 600 meters The C Soft Windows based user interface allows for intuitive calibration data loggings set up and file downloading Existing Cyclops 7 users can easily integrate their sensors into the C6 Multi Sensor Platform Available Cyclops 7 Sensors In Vivo Chlorophyll a Blue Green Algae Phycoerythrin Blue Green Algae Phycocyanin CDOM Turbidity Crude Oil Refined Oil Fuels Rhodamine Dye Fluorescein Dye See note below Optical Brighteners gt gt gt gt gt gt lt S lt lt X lt 5 5 Custom Optics Important Note Regarding Use of Fluorescein Sensor The Fluorescein sensor when enabled with other sensors specifically Chlorophyll and Rhodamine sensors for simultaneous data collection will produce unreliable results Therefore the Fluorescein sensor should not be used for simultaneous data collection with Chlorophyll or Rhodamine sensors All other sensors can be used simultaneously with the Fluorescein sensor 998 2205 Rev E Page 5 G Platform 2 0 Inspection and Setup 2 1 Instrument Checklist The C6 Multi Sensor Platform package P N 2200 000 includes C6 Multi Sensor Platform Factory installed pressure sensor Factory installed temperature sensor Interface cable for PC with 12V power supply 4 Sensor fasteners 8 pin female plug 4 CD includes C Soft s
14. TURNER DESIGNS Reliable Instruments for an Unreliable World Multi Sensor Platform User s Manual a a Sensor PIANT A a a H TM j NER Serin W 2200 10 Sao 100 EL y 2200 20 pda March 30 2010 P N 998 2205 Revision E TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 Multi Sensor Platform Table of Contents TODO CONTA AA AAA AA e N AN 2 1 0 TA A O 5 Important Note Regarding Use of Fluorescein Sensor cccccccccccccc 5 2 0 INSPECIION GNA MU nds dado ee bo 2 de Tavstriment CAE a aaa atid dn tats oe bacat 6 E SLAC d sE ven E ene ene a eee es 8 221 installing Cyclops 7 Sensors a a a tain Asien 8 2 2 2 nstalling the Mechanical Wiper anta a 9 OV CE VIG A Oe eres 9 DENCO Ree enn Pie ore te ere eee ere MNT ete eee eee cee err ree Te eer ete 9 BEDS CAMEO ease eae E T E EREET A A E T ias 9 220 Installing the POC ii 10 YA nn A acnc onertes onaiaie nannies 10 InStallatiOR eerie inane olla iii 10 JC OE Windows US CRIT CO ii A Aia ao 11 3 1 Software Installation and PC Requirements ooccccccnnnnnnnnncnnnnnnnnonnnnnnnnnnnnnnnnnnononnnnnnnnnnnnnnnnnnnnnnnnnos 11 Sd Viimua PE Requirements a a lausutenciddeseavaatunina hd tawartinedececeauie 11 Dalle Z SOR Wale Installation a a a a a e a a de a 12 FESP SOR ae terracota nado aia 12 D2 SOM Wale Opera ado cola 13 A A Coreen E A ice nai aac lab tena E EA A SR A
15. Unit Clock 998 2205 Rev E Page 13 Multi Sensor Platform 3 2 2 Current Data and Calibration Screen The Current Data Screen allows users to view real time data acquisition in both tabular and waveform formats as well as conduct calibrations for each channel Current Data 4 4 Sensor labels are displayed but cannot be changed in the Current Data screen Real time values are displayed next to each channel and in the adjacent Data Table A calibration button 1s available for all Cyclops 7 sensors and the depth sensor Real time data can be saved by selecting the Start Data Capture button Users will be prompted to enter a file name and file location Data files will be saved in csv format Tabular Screen 4 Real time data including time date and units are continuously scrolling on the tabular screen for enabled sensors Each sensor identification name will appear in the associated column headings If units are not selected during calibration a relative fluorescence units RFU label will appear Ae File Settings COMPort Window Help Connected Q Data Table Display Waveform I Date Time Rhodamine Phycocyanin Depth Tem oe Settings Current Data LogSetup Download Data l i HA REN Y ae is P A 06 16 08 10 34 57 69 08 78 72 0 00 21 47 0
16. ensor Platform Operating Instructions Description The Turner Designs C6 Multi Sensor Platform can be used with Cyclops 7 sensors for many different applications It can also be integrated into a multi parameter system Itis capable of logging and storing data and uses algorithms to compute direct concentrations of fluorophores Identification e Each Cyclops 7 Sensor is identified by a letter stamped into the connector C Chlorophyll U CDOM O Crude Oil F Fluorescein B Optical Brighteners P Phycocyanin E Phycoerythrin R Rhodamine T Turbidity and G Refined Oil Fuels Recommended Measurement Practices The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a Glass Beaker for your water samples Avoid plastic beakers plastic may fluoresce and might interfere with measurements 2 Place the glass beaker on a non reflective surface preferably black 3 Ensure that the sensor is more than 3 inches above the bottom of the glass beaker 4 Ensure that the sensor is in the center of the glass beaker and has more than 2 inches clearance between the sensor and the inside surface of the beaker 5 Check that the optical surface of the sensor is free of air bubbles IE a gt 2 inches all Glass Beaker gt 3 inches No Air Bubbles On Optical Surface
17. entration mode raw fluorescence mode or blank subtracted raw fluorescence mode C Soft Manager 10 x Channel Calibration Unit Label Chan 1 Rhodamine Current RFU 83 20 Step 1 Press Set Blank to set the blank Blank RFU value to the current RFU x1 Set Blank x10 x100 Step 2 Enter the value of your standard Standard Value UNE and select the units of measure Step 3 Press set Standard to set the Standard RFU standard value to the current Set Standard RFU Step 4 Optional Click the temperature Temp Compensation compensation box to activate a Step 5 After calibration is complete press Save Calibration Save Calibration Cancel Click to use uncalibrated Raw Fluorescence Mode Direct Concentration Mode Values reported in the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted The equation used to calculate concentrations after calibrating to Direct Concentration Mode is Standard Value units x RFU Response Concentration units Standard RFU Raw Fluorescence Mode Blank Subtracted Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB It is important to note that these values are blank subtracted but are not scaled to a standard they are relative values Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Relative Fluorescence Units RFU It is important to note that these values are not
18. form 4 1 Connecting the Submersible Battery Pack 4 0 Deployment The Battery Pack comes with a 12 inch cable and a charger The 12 inch cable is not needed if the optional battery bracket is installed The battery is fully charged after 8 hours After data logging is enabled attach the battery to the C6 Multi Sensor Platform with the 12 inch cable or via battery bracket Secure C6 Multi Sensor Platform and battery to a fixed structure Data logging will begin at specified time and date Note The battery is rated for 10 000 data lines each data line can range from 3 9 data points 4 2 Battery Bracket Installation The optional battery bracket was designed for easy self contained compact deployment of the C6 Multi Sensor Platform The 12 inch cable is not needed if the battery bracket is installed 1 Remove the 2 eyelifts located at the top of the C6 Multi Sensor Platform housing using a screwdriver 2 Align the bracket holes with the threaded eye lift holes 3 Using the screws included in the bracket package fasten the brace to the C6 Multi Sensor Platform housing 4 Glide the battery pack through the brace towards the C6 Multi Sensor Platform 8 pin connector 5 Align the 8 pin connector and battery and secure Ensure the battery is completely mated before next step 7 After installing the battery pack use a hex key to tighten the battery ring A Tighten ring with hex key to grip the battery pack 998
19. gs in the Log Setup section 3 2 3 and again enable datalog to put the instrument into the Data Output Ready State When power is supplied to the instrument while it is in the Data Output Ready State it will begin streaming data after a 100 second delay If power is cycled 1 e disconnected and reconnected please allow 100 seconds for the data stream to begin The C6 Multi Sensor Platform s standard output is in digital ASCII format Integration Cable for Digital ASCID Output The Integration Cable is recommended for digital integrations with CTDs or Third Party Platforms This cable is designed so that flow control is disabled therefore it may only be used for integrations and cannot be used for communicating with the C3 As long as the C3 is in the Data Output Ready State power is supplied to the instrument and a 100 second delay has been allowed the C3 will output digital data continuously without interruptions if the integration cable is used Using any other cable for digital integrations may cause interruptions caused by hardware flow control The Integration Cable has 3 connectors 1 A serial DB9 plug RS232 2 A female plug for supplying power to the instrument 3 An 8 pin female connector for connecting to the C3 And can be ordered in either a 2 foot PN 2200 160 or 5 meter PN 2200 165 cable length Note The Integration Cable will have a yellow band which distinguishes it from the standard Interface Cable used to com
20. intervals in the HyperTerminal window after a 100 second delay or at the specified log start time C Soft HyperTerminal jE x File Edit view Call Transfer Help RhodaminePhycocyani Depth Temp C 90 65 129 70 0 00 23 71 91 09 130 71 0 00 23 72 91 01 130 83 0 00 23 72 31 23 130 93 0 00 23 72 O 91 09 130 95 6 00 23 72 6 16 08 15 07 29 91 01 130 92 0 00 23 72 6 16 08 15 07 30 31 23 131 10 0 00 23 72 Most applications will integrate streaming data Below are instructions on how to save streaming data on a PC 998 2205 Rev E Page 24 Multi Sensor Platform To save data 1 On the HyperTerminal window toolbar choose Transfer and then Capture Text File Edit view Call Transfer Help Pla el el a 35 fa Send File Receive File Capture Text Send Text File Capture to Printer 2 When you click on Capture Text a window will appear asking you to name and save the txt file to the location of interest Folder C Documents and File SEA TURNERDESIGNSACAPTUAE TST Browse 3 Once you are finished you will need to return to the HyperTerminal window toolbar choose Transfer and then Capture Text and select Stop This will stop data capture to the txt file File Edit wiew Call Transfer Help l a B af Send File Receive File Capture Text Send Text File Pause 5 22 Resume Capture to Printer T pa 998 2205 Rev E Page 25 Plat
21. mage incurred in shipping is not covered 5 5 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 998 2205 Rev E Page 27 Multi Sensor Platform 3 If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 5 6 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist y
22. municate with the C3 Submersible Fluorometer After the instrument has been calibrated proceed to Log Setup Section 3 2 3 After logging has been enabled the C Soft software will automatically shut down Users should disconnect power and communicate with the C6 Multi Sensor Platform via HyperTerminal serial terminal emulator 998 2205 Rev E Page 23 Multi Sensor Platform 3 2 6 How to setup Hyperterminal for Digital ASCII Data Capture l 5 On a MS Windows computer open the HyperTerminal program Start All Programs Accessories Communications HyperTerminal The Connection Description screen will appear Name the connection description This file will save the communication parameters for the C6 Multi Sensor Platform and can be used in the future to establish communication with the instrument quickly The Connect To screen will appear Choose the appropriate communications port in the Connect Using window Click OK The Port Settings screen will appear Choose the following port settings Bits per second 9600 Data bits 8 eee Hx Port Setti Parity None e Stop bits 1 Flow control Hardware Click Apply and OK Bits per second EMIN Data bits E Parity None Stop bits fi Flow control Hardware Restore Defaults caos toy The HyperTerminal window will appear You are now ready to connect C6 Multi Sensor Platform to a power source Data will be displayed at set
23. nisms on the sensor optics The Solid Secondary Standard provides a very stable fluorescent signal It has an adjustment screw so that you can tune the Solid Standard to provide a signal to match a specific sample Installing the Secondary Standard 1 Before installing the Solid Standard you must ensure that the optical surface of the Cyclops 7 is completely clean and dry The Solid Standard is indexed and it must be installed so that the indexing is precisely aligned for proper use see below 2 To install place the Solid Standard on to the optical end of the Cyclops 7 Cyclops 7 Solid Standard indexing mark Align the index mark and indexing ball when installing Cyclops 7 in the Solid Secondary Standard Insert the supplied green screwdriver through the calibration hole in the locking nut to reach the adjustment screw Adjustment screw is located under the locking nut 998 2205 Rev E Page 33 Multi Sensor Platform 3 With the Solid Standard fully mated with the sensor head rotate in either direction until you feel the Solid Standard indexing ball click into the indexing mark on the Cyclops 7 4 To begin use a flat head screwdriver to unscrew the locking nut as far as 1t will go 5 Next to change the signal level use the green screwdriver provided and insert the blade through the hole in the locking nut Rotate it until it engages with the adjustment screw that is beneath the locking nut Now the screw can be
24. oftware user s manual and quick start guide Temperature sensor Sensor fastener Eyelift holes Nx li il o gt gt l Ne 2200 009 i gue DESIONS i of 23m ei 8 pin female end plug Pressure sensor 12V power supply Computer interface cable 6 pin male plug 998 2205 Rev E Page 6 Platform Optional Accessories 4 gt gt Submersible Battery Kit includes charger P N 2200 601 Turner Designs Mechanical Wiper P N 2200 450 and Replaceable Brush P N 2200 460 Submersible Battery Bracket P N 2200 603 6 pin male plugs P N 105 2210 2 foot Integration Cable P N 2200 160 or 5 meter Integration Cable P N 2200 165 Flow Cap P N 2200 700 Shade Cap P N 2200 500 Booster Kit P N 2200 900 Note Booster Kits are required for cable lengths greater than 10 meters Extended Cables 10 meters P N 105 2595 25 meters P N 105 2596 50 meters P N 105 2597 Custom lengths available upon request gt ICO Booster 1 Submersible battery 998 2205 Rev E Page 7 Multi Sensor Platform Shade Cap Mechanical wiper i Battery bracket 2 2 Getting Started 2 2 1 Installing Cyclops 7 Sensors Up to six Turner Designs Cyclops 7 sensors can be installed into the C6 Multi Sensor Platform The center port is intended for the optional mechanical wiper only Follow the instructions bel
25. ons 2 The second compensates for gravity variations with latitude as designed from the UNESCO Technical Papers in Marine Science 44 This method assumes 0 C and 35PSU salinity ioj x Channel Calibration Unit Label Depth Current Pressure 0 00 PSI Step 1 Press set Zero to set the Zero Reading atmospheric zero Set Zero Step 2 Select a pressure compenstion method Atmospheric offset only Atmospheric and gravity offset Step 3 When you are done press Save Calibration or press Cancel at Save Calibration Cancel any time to abort this calibration Atmospheric Offset Step 1 Method 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Method 1 Select Atmospheric offset only Step 3 Method 1 Select Save Calibration button 4 Future depth values will be scaled to the offset Atmospheric and Gravity Offset Step 1 Method 2 998 2205 Rev E Page 19 Multi Sensor Platform Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Method 2 Select Atmos
26. ou by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2205 Rev E Page 28 Ge Platform Equipment Specified as Electrical and Electronic Waste 998 2205 Rev E Page 29 Multi Sensor Platform Appendix A Specifications Weight in Air 2 7 kg 6 0 lbs without sensors submersible battery or wiper Weight in Water 1 6 kg 3 4 Ibs Length 25 8 cm 10 2 in Length with Sensors Attached 33 8 cm 13 3 in Diameter 10 2 cm 4 0 in Material 316 Stainless Steel Delrin Temperature 2 to 50 degrees C Depth 600 meters External Power 8 to 30 VDC Signal Output Interface RS232 USB Adaptor Digital Maximum Sampling Rate 1 second Nonvolatile Memory 64 000 Data Lines Submersible Battery Pack Battery Lithium lon Weight in Air 0 75 kg 1 65 Ibs Weight in Water 0 22 kg 0 50 Ibs Length 13 46cm 5 3 in Diameter 7 cm 2 75 in Material Delrin Temperature 2 to 50 degrees C 600 meters 2 200 mAH Depth Capacity Voltage Output 14 8 V nominal Protects against overcharge over discharge overheating Protection Features Ses short circuiting 998 2205 Rev E Page 30 Multi Sensor Platform Appendix B C6 Multi S
27. ow to successfully integrate your Cyclops 7 sensor 1 4 5 Place the fastener on one of the Cyclops 7 ports of the C6 Multi Sensor Platform and rotate to tighten Make sure not to over tighten Align the 6 pin male end of the Cyclops 7 sensor with the C6 Multi Sensor Platform port and fastener Hold the Cyclops 7 in place and rotate the remaining ring to tighten This will lock the Cyclops 7 into position Follow the same installation instructions for the remaining Cyclops 7 sensors Install a 6 pin male plug into any unused port on the C6 Multi Sensor Platform The center port is for the mechanical wiper only 998 2205 Rev E Page 8 Multi Sensor Platform Cyclops 7 Fastener 2 2 2 Installing the Mechanical Wiper Overview The C6 Multi Sensor Platform s mechanical wiper is an optional accessory that can be easily installed to eliminate bio fouling during extended deployments The wiper uses non destructive brush material to clean Cyclops 7 sensors allowing for accurate readings under extreme bio fouling conditions Users are able to select the number of wiper rotations before sampling Benefits Limits debris and bio fouling organisms from interfering with readings Easy installation Stainless steel housing prolongs motor life Easy brush replacement design Adjustable wiping revolutions Installation The mechanical wiper has a 6 pin male end that fits into the center port on the C6 Multi Sensor
28. pheric and gravity offset Enter the latitude Step 3 Method 2 Select Save Calibration button Future depth values will be scaled to the offset 3 2 3 Log Setup Screen The Log Setup screen allows the user to configure a logging session activate the wiper and enable logging The C6 Multi Sensor Platform can log just over 64 000 lines of data Enter a log start date MM DD Y Y Y Y and time HH MM SS C Soft Manager 4 File Settings COMPort Window Help Connected Settings Current Data LogSetup Download Data Log Timing Log Start 10 24 15 06 16 08 Sampling Interval HH MM SS 0 00 30 Wiper Setup Wiper I Revolutions 1 vi Test Wiper Enter the sampling interval HH MM SS The minimum sampling interval is 1 second 998 2205 Rev E Page 20 Multi Sensor Platform If the bio fouling wiper is installed click the Wiper box to enable Select the number of revolutions from the dropdown box 1 10 revolutions All sensors will be wiped at specified revolutions before each measurement Note If users select revolutions that exceed sampling interval a warning message will be displayed C Soft Manager x The sampling interval is too short for the number of wiper revolutions You must choose a longer sampling time less revolutions or turn the wiper off Sampling Maximum Interval of Revs 1 10 O 11 20 1 21 30 E ap 4 3 41 50 q 31 50 a gt 6U 1
29. r further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll a please refer to the application section of Turner Designs web site at the following URL http www turnerdesigns com esupport understanding html 998 2205 Rev E Page 35
30. ration cup for holding standard solutions when calibrating Cyclops 7 units The flow cap also offers protection for Cyclops 7 units during deployment or transportation and will help limit bio fouling Installation 1 Use a screwdriver to remove the screws that fasten the C6 Multi Sensor Platform s support bracket 2 Remove the C6 Multi Sensor Platform s support bracket 3 Unscrew the 3 stainless steel rods attached to the C6 endcap 4 Position and mate the base of the Flow Cap to the C6 endcap 998 2205 Rev E Page 10 Ge Platform 5 Screw in the 3 stainless steel rods into their original positions on the C6 endcap 6 Turning clockwise fully thread the Flow Cap housing onto the Flow Cap base connector 7 Turning clockwise thread the Flow Cap s endcap to the Flow Cap housing until it is fully hand tightened Jo In Flow Valve ma T Note Turner Designs recommends the above flow design for the Flow Cap It is recommended to position the instrument vertically with the sensor head facing upward to expel any air from the system that might cause skewed readings 3 0 C Soft Windows User Interface The C Soft Windows based user interface allows for intuitive calibration data loggings set up and file downloading 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 133MHz microprocessor 600 X 800 VGA resolution Microsoft Windows 95 Y 32MB RAM
31. rest i e de ionized water artificial seawater or filtered seawater Note Ensure the Cyclops 7 sensors are free of bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Future measurements for this sensor will be blank subtracted based on the blank response recorded during calibration Step 2 Selecting Units Select RFUB from the units dropdown menu Note Do not enter a value in the standard value box Step 3 Skip and go to Step 4 Step 4 Optional Temperature Compensation Follow same steps under the Direct Calibration procedure Step 5 Save Calibration 4 After calibration is complete select Save Calibration Data for the selected sensor will now be in relative fluorescence units and blank subtracted 998 2205 Rev E Page 18 Multi Sensor Platform 3 2 2 3 Raw Fluorescence Calibration In the Current Data screen select the calibrate button next to a specific Cyclops 7 sensor At the bottom of the Calibration Screen click to use Uncalibrated Raw Fluorescence Mode Data for that sensor will be uncalibrated and reported as Relative Fluorescence Units RFU 3 2 2 4 Depth Calibration Depth is displayed in meters only There are two calibration options for depth calibration 1 The first uses only the atmospheric pressure as an offset This is suitable for any shallow application including most fresh water applicati
32. rm Operating Instructions oooccccnnnnnnnnnnnccnnnnnononanoconnnnnonananacinnnnss 31 Descrip omesse aE EAE SE 31 CUTAN GING ACN OWN ssena ies 31 Recommended Measurement Practices oooooooooooncncccccnncnnnnnnnnnnnnnos 31 Appendix C C6 Multi Sensor Platform Wiring Guide ooooooonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnos 32 Appendix D Using the Secondary SAN ATd CAE 33 Solid Secondary Standard Benefits oooooocccnnnncnnooccnnnnnnnnoconnnnnnnos 33 The Solid Secondary Standard provides a very stable fluorescent signal It has an adjustment screw so that you can tune the Solid Standard to provide a signal to match a specific sample Installing the Secondary Standard 33 Use of the Solid Secondary Standard o oooooccnnnnnnnocccnnncnnnococnnnnanononos 34 Linear Range and Quenching occcccccncnccnccnnnnnonononononncnnncnnnnnnnnnononnnnnos 35 Temperature Considerations ooooccccnncccnncononccnnnnnnnnononononnnnnnnnnnnnnnnnocnnos 35 Ge Platform Equipment Specified as Electrical and Electronic Waste Multi Sensor Platform 1 0 Introduction The C6 Multi Sensor Platform was designed to integrate up to six Cyclops 7 submersible sensors for extended or short term deployments The C6 Multi Sensor Platform provides automatic gain control calibration digital data reporting data logging and digital data output for each Cyclops 7 sensor The C6 Multi
33. used to adjust the signal level as necessary 6 Once the desired reading has been obtained the locking nut should be screwed down so that the adjustment screw is held firmly in place 7 Note that the response of every solid standard is unique Hence a new correlation must be determined for every sensor For future identification use the ID space on the label for a unique identifier for the Secondary Standard Use of the Solid Secondary Standard The Solid Secondary Standard can be use to Check signal stability Determine drift Recalibrate 1 To establish a correlation between a known concentration and it s respective fluorescence output immerse the sensor in a sample with a known concentration of your fluorophore 2 Dry off the C3 Submersible Fluorometer s optics attach the Solid Standard and adjust the Solid Standard to produce the same fluorescence output value from the sensor as in step 1 turning the Secondary Standard adjustment screw clockwise produces a higher signal 3 Now your Solid Secondary Standard has been correlated to a known concentration and as long as it is no longer adjusted stored and used properly that signal will remain a stable reference point 4 Comprehensive information on fluorescence applications http www turnerdesigns com t2 doc appnotes main html fluorescent Note There are no Solid Secondary Standards available for the Turbidity or UV optical sensors 998 2205 Rev
34. y or select desired units from the dropdown menu Note Do not choose or enter RF UB if Direct Concentration Mode is used Step 3 Set Standard When readings in Current RFU box have stabilized select the Set Standard button Step 4 Optional Temperature Compensation Temperature compensation is optional and allows the user to automatically compensate to the factory installed temperature sensor Temperature compensation is available for Chlorophyll a and Rhodamine sensors only Temperature is displayed in degree Celsius Click on the Temperature Compensation box Select either Chlorophyll a or Rhodamine fluorophore from the dropdown menu Data will now be corrected for temperature fluctuations 998 2205 Rev E Page 17 Multi Sensor Platform The temperature compensation coefficients for each fluorophore are listed below Fluorophore Coefficient WN Chlorophyll a 1 4 per degree C Step 5 Save Calibration After calibration is complete select Save Calibration Data for the selected sensor will now be calibrated 3 2 2 2 Raw Fluorescence Calibration Blank Subtracted Following steps 1 5 will ensure values are reported as Blank subtracted Relative Fluorescence Units RFUB See Appendix B for recommended practices Step 1 Blanking Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a blank solution Note A blank solution is a solution without the fluorophore of inte
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