JGC-3, JGC-2, and JGC-4 Gel Casters User Manual
Contents
1. front of the bag 8 Tighten the screws using moderate tension This is to prevent the liquid in the bag from bulging Figure 2 2 Secure with Screws JGC Gel Caster 2 1 Section 2 Setting Up Gel Casting 9 Use the two front screws on the base continued of the unit to level the casting stand 10 Pour Liquid can be pipetted in or poured in Figure 2 3 11 Overlay the gel with water or water saturated isobutanol as usual and Figure 2 3 Pour Slowly allow to polymerize 12 The pouch can now be heat sealed for later use Just rinse off the isobutanol and replace with buffer 13 If required the stacking gel can be poured in or out of the bag If the comb fits the casette tightly it may be better to pour this gel while the gel is clamped to the running device This mitigates the possibility of separating the plates from the gel 14 Remove the gel from the pouch Squeeze from the bottom if there is a tight fit 15 Rinse the cassette with water Add from buffer to the wells if present 2 2 JGC Gel Caster Thermo Scientific Section3 Technical Tips Combs STANDARD 0 5mm A 0 8mm and 1 5mm D thicknesses STANDARD PREPARATIVE One long well and one marker lane Figure 3 1 Combs CUSTOM COMBS Call Technical Services for more information Spacers STANDARD Protein spacer sets include two side spacers and one bottom spacer Spacers and combs must be of identical thickness to be used toget2. 03 1 liter bottle 7005 4 liter bottle Rnase AWAY is a registered trademark of Molecular BioProducts Thermo Scientific JGC Gel Caster 5 1 Section 5 Care and Cleaning 5 2 Care of Acrylic JGC Gel Caster The following chemical compatibility chart is supplied for the convenience of our customers Although acrylic is compatible with most solvents and solutions found in the biochemical laboratory some solvents can cause substantial damage Keep this chart handy to avoid harm to your apparatus by the use of an inappropriate solvent Codes 5 Safe no effect except possibly some staining A Attacked slight attack by or absorption of the liquid Slight crazing or swelling but acrylic has retained most of its strength U Unsatisfactory softened swollen slowly dissolved D Dissolved in seven days or less Thermo Scientific Section 5 Care and Cleaning Table 5 1 Chemical Compatibility for Acrylic Based Products EA 20 Mesum dS penus A inte EEC EC UN EI je heces i messa IN e e qn mee e eem fen qoem p opem p penam eA Ur rem cion essen ue rar e EE Bee n mem DN EC S Bem DI ET fu EI s Carbon tetrachloride 0 Hydrogen peroxide 28 solution U Sodium hydroxide 60 fS CESTO CN CTO DN EI nen u O CECI DN ECON DN ECO E Ro EX jun ETC Ten je o LN O Deme qr nem o eee je Bey Ta quee fe am fe 1 This list does n
3. 10cmW x 10cmL N A N A P7 10FR 10cmW x 10cmL N A N A P7 10FG 10cmW x 10cmL N A N A P7 10RA 10cmW x 10cmL P7 SA P7 SC N A JG4 PL JGC4 006 GP4 25 GP4 100 R10013 R10416 P1 14G 16cmW X 14cmL P1 14R 16cmW x 14cmL P1 14FG 16cmW x 14cmL P2 18G 20cmW x 18cmL 1 CS SD JGC Gel Caster 6 1 Section 6 Optional Equipment 6 2 JGC Gel Caster Table 6 3 Combs Options Model JCG 4 Catalog Comb Type Number of Thickness of Width of EST Well Number Teeth Tooth Teeth Volume ul CNN NN CNN C M je o 5 co S gt N 8 0 m CC v CA Table 6 4 Combs continued Number Type Teeth Tooth mm Teeth mm Volume ul pog e ue E Expo Me e EIE DOE cn CNN RN NN CR CR RN Well e is TU gt gt 1 1 e e CNN RN CNN NN p B R ps p R Thermo Scientific Section 6 Section title Table 6 5 Combs continued EX Thickness of Width of EST Well Number Type Teeth Tooth mm Teeth mm Volume ul pe pe w je fi pe ee pw CU CA E pm w p fi pw CN je fi Thermo Scientific JGC Gel Caster 6 3 1006 OSI 216 0 AY uoneuuoJul AJUCIIEM JOINGLISIP 890 Jno 19641009 YSN eui episino elo eds pue sonas uoneJedo AjueJem Juswd nba uo suonsanb n
4. C Gel Caster Thermo Scientific Thermo Scientific Gel Casting Section 2 Setting Up The JGC Gel Caster is not designed to seal off the bottom of the gel rather restrict where the gel solution can go The gel solution is forced to occupy only the space between the glass plates There is some room in the pouch which will also fill with gel solution Liquid will come out from between the plates but the bag restricts where it can go The bottom of the pouch plastic bag willl form a v of gel that will exclude air from between the plates On the side of the cassette there will be a worm of gel formed that you can pull or rinse off It is a good idea to rinse the gel cassette clean glass creates a better seal against the gasket of the running device 1 Open pouch and insert one blank plate and a spacer placer 2 Add one notched plate on top of the spacer placer so that the spacer placer is between the two plates 3 Insert a spacer one on each side Figure 2 1 Inserting into Pouch of the spacer placer Repeat Steps 1 3 for a second gel cassette One or two cassettes will fit into a single pouch 4 Press the spacers in toward the center of the spacer placer 5 Let the bag and its contents rest on the lab bench while lightly holding the bag by the sides This will let the spacers and both pieces of glass align their bottoms together 6 Place the bag up against the back of the unit 7 Place the blocking plate in
5. Gel Caster Models JGC 3 JGC 2 and JGC 4 Operating and Maintenance Manual 7217692 Rev 0 Visit us online to register your warranty w www thermoscientific com warranty Owl 6 E separation Preface MANUAL NUMBER 7217692 0 5 7 12 Transfer to Marietta was The Joey 11 2003 CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific JGC Gel Caster i Preface Contains Parts and Assemblies Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance A Caution internal adjustments and maintenance must be performed by qualified service personnel Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo Fisher Scientific be held liable for any damages direct or incidental arising out of or related to the use of this manual Statement of Proper Use Use this product only for its intended purpose as described in this manual Do not use this product if the power leads are damaged or if any of its surfaces are cracked 2012 Thermo Fisher Scientific All rights reserved ii JGC Gel Caster Thermo Scientif
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7. e Step 2 in Section 2 This is normal the bead of gel will exclude air bubbles from being trapped between the glass plates Excess polyacrylamide may polymerize between plates Using a razor blade and gently placing between each of the gel assemblies will loosen the acrylamide Gently apply pressure to separate the gel assemblies and wash glass of any excess poly acryamide Or use one gel per pouch JGC Gel Caster 4 1 Section5 Care and Cleaning Caution Organic solvents cause acrylic to craze or crack Clean all acrylic systems with warm water and a mild detergent Do not use ethanol or other organic solvents to clean acrylic products Do not autoclave bake or microwave your unit Temperatures over 50 C can damage the acrylic A Note If an RNase free electrophoresis system is desired there are various methods to rid the system of RNA contamination For fast and easy decontamination use RNase AWAY Spray wipe or soak labware with RNase Away then wipe or rinse the surface clean it instantly eliminates RNase RNase Away eliminates the old methods that include treatment with 0 1 Diethyl Pyrocarbonate DEPC treated water and soaking in dilute bleach DEPC is suspected to be a carcinogen and should be handled with care This electrophoresis system should never be autoclaved baked or placed in a microwave A To order RNase AWAY contact Technical Services Part Number 7000 250ml bottle 7002 475ml spray bottle 70
8. her WEDGE Linear wedge spacers 0 4 0 8mm provide a current gradient allowing a single percent gel to separate disparate sized DNA fragments Thermo Scientific JGC Gel Caster 3 1 Section 3 Technical Tips 3 2 Reagent Information Preparation SDS PAGE Continuous Buffer System JGC Gel Caster Glass Polyacrylamide Electrophoresis buffer 0 1 M sodium phosphate pH 7 2 0 1 w v SDS The columns represent volumes ml of stock solutions required to prepare 30ml of gel mixture BLANK The plate which faces you during electrophoresis All gel sandwiches require one piece of blank glass NOTCHED The plate which faces the chamber during electrophoresis Spacers are placed over the ears of the plate when casting vertical gels Buffer accesses the gel between the ears OFFSET Offset plates may be used in place of notched plates They require sponge tips mounted on the spacers Sponge tips take the place of the ears and prevent buffer from running out of the upper buffer chamber from the sides OFFSET FROSTED Figure 3 2 Glass Frosted plates are used for vertical agarose electrophoresis One side of the plate has a rough surface to prevent agarose from sliding down Thermo Scientific Offset vs Notched Thermo Scientific Glass Section 3 Technical Tips All units require a blank piece of glass and an offset or notched piece of glass Offset glass is glass that is about 2cm sh
9. ic gt ag D SS 9 Ee Preface Important operating and or maintenance instructions Read the accompanying text carefully Potential electrical hazards Only qualified persons should perform procedures associated with this symbol Equipment being maintained or serviced must be turned off and locked off to prevent possible injury Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientifics compliance with this directive the recyclers in your country and information on these products will be available at www thermofisher com Y Always use the proper protective equipment clothing gloves goggles etc v Always dissipate extreme cold or heat and wear protective clothing v Always follow good hygiene practices Y Each individual is re
10. ine Aes reale 5 2 Optional Equipment eee eee eee 8B 6 1 Accessories erh 6 1 Comb Options a RR PN 6 2 JGC Gel Caster V Thermo Scientific Section 1 General Information This patented Gel Casting System is a unique method to cast 1 to 4 poly acrylamide gels without taping or special sealing of the gel plates Plastic pouches hold glass plates and spacers snugly together in the casting stand These pouches serve as the bottom spacer and a containment system for any excess polyacrylamide Gels may be cast ahead of time and sealed inside the plastic pouch creating your own pre cast gels Before starting unpack the unit and inventory your order Reference the order or catalog number on your invoice and check the corresponding parts list Table 1 1 If any parts are missing refer to the service page of this manual and contact Technical Services Table 1 1 Parts List Table 1 2 Specifications Unit Model Number JGC 4 JGC 2 JGC 3 10cmW x 10cmL 16cmW x 14cmL 10cmW x 8cmL 16cmW x 16cmL Footprint WxD 13x13 17x23 10cmW x 10cmL 6cmW x 14cmL Glass Size cm W x L 10cmW x BemL 20cmW x 20cmL Compatible with Unit P8DS P81 P82 P9DS JGC Gel Caster 1 1 Section 1 Care and Cleaning SPACER PLACER SPACERS OPTIONAL JOEY BAG SPACER PLATE CASTING STAND THUMB SCREW 2 BUBBLE LEVEL STABILIZING SCREW 4 Figure 1 1 Exploded Parts Diagram 1 2 JG
11. necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor iv JGC Gel Caster Thermo Scientific Thermo Scientific Section 1 Section 2 Section 3 Section 4 Section 5 Section 6 Table of Contents General Information nun nenn 1 1 Parts ce ROG EMO Sue eh ewe i 1 1 ON Ss a re UE uA E 1 1 Exploded Part Diagram A Reet whe 1 2 Setting UN cr trab amen oe 2 1 ONG Ton M TC TL 2 1 Technical Tips lx ia 3 1 FONDS a d O Ne EHE 3 1 Nro Pe OT 3 1 Reagent Information viis eod TA an 3 2 Co na ee es er AA 3 2 Offset vs Notched Glass 5 od A A RD IN 3 3 Troubleshooting Luuueseseeee nennen nn 4 1 Care and Cleaning 0 ccc cece cece cece eee nennen 5 1 Creolen edo S
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13. orter than the blank piece without ears on the sides Notched glass has two ears that are left behind when a cut is made in the middle of the top of the glass Both offset glass and notched glass SPONGE TIP allow the gel and samples to make contact with the upper buffer chamber Offset glass has to be used with sponge tips which take the place of the a notches on the glass The advantage of offset glass is that this glass is more rigid Notched glass is easier to use and does not require the addition of sponge tips Figure 3 3 Spacer JGC Gel Caster 3 3 Thermo Scientific Section 4 Troubleshooting PROBLEM polyacrylamide is leaking from between glass plates when pouring Gels are not level Bag is leaking Spacers won t line up Excess polymerized polyacrylamide cassette s from the bag Gels stick together inside of bag SOLUTION Be sure the acrylic blocking plate is placed in front of the glass and bag assemblies before tightening the bags into the casting stand This spacer plate provides even pressure across the entire glass plate Also be sure the spacers and glass plates are pushed firmly to the bottom of the casting stand Use the leveling screws on the base of the casting stand to level the caster Replace glass in new bag and re pour gel Check bags with the gel in place with water Some bags may have small holes and or the glass can create small cuts Use a spacer placer to align the spacers Se
14. ot include all possible chemical incompatibilities and safe compounds products should be cleaned with warm water a mild detergent such as Alconox and can also be exposed to a mild bleach solution 10 1 In addition RNAse removal products are also safe for acrylic Thermo Scientific JGC Gel Caster 5 3 Section 6 Optional Equipment JGC Gel Caster units are sold without accessories to allow for maximum flexibility All accessories need to be purchased separately In addition to the stock items listed below custom glass spacers and combs can be made for these units Call Technical Services for details Table 6 1 Accessories Description Blank Glass Plates 3 32 Thick Blank Glass Plates 3 16 Thick Blank Glass Plates 1 8 Thick Notched Glass Plates 3 32 Thick Notched Glass Plates 3 16 Thick Notched Glass Plates 1 8 Thick Frosted Notched Glass Plates 3 32 Thick Frosted Notched Glass Plates 3 16 Thick Frosted Notched Glass Plates 1 8 Thick Frosted Blank Glass Plates 3 32 Thick Frosted Blank Glass Plates 3 16 Thick Frosted Blank Glass Plates 1 8 Thick Offset Glass 3 16 Thick Offset Glass 1 8 Thick Notched Alumina Plates 1 0mm Thick Spacers 0 5mm Thick Spacers 0 8mm Thick Spacers 1 5mm Thick Spacer Placer pkg of 3 Blocking Plate Gel Pouches 25 pouches Gel Pouches 100 per package Thumbscrew Bubble Level Thermo Scientific JGC 4 P710G 10cmW x 10cmL N A N A P7 10R
15. sponsible for his or her own safety Thermo Scientific JGC Gel Caster iii Preface Do You Need Information or Assistance on Thermo Scientific Products If you do please contact us 8 00 a m to 6 00 p m Eastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermoscientific com Internet Worldwide Web Home Page service led marietta thermofisher com Tech Support Email Address www unitylabservices com Certified Service Web Page Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical information about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is
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17. xe s ouueu peoejdoiJ IM diusueunjoM Jo ui 2 aq ue oJd syed sujuoui 92 xis ALU 18414 ayy Bung Jeuwo juenbesqns Aue uon 98 01d JUeJJem ay si juaudinba noA au y Pays OU uenem ay os eui Buiddius siu Apre uno peddius si ajep eui OM 5 pollag SUL TWNOLLVNYSLNI ALNVSHVM SLINCOYd IMO 9131133195 S3HSI3 OINS3HL Fisher Scientific The Joey Gel Caster 1 2 Thermo Fisher Scientific 401 Millcreek Road Marietta Ohio 45750 United States www thermofisher com ThermoFisher SCIENTIFIC
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