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Multiskan GO User Manual - Fisher Scientific

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1. Results Function keys Aa or Alphanumeric keypad pgrs tuv Arrow keys and OK key START ON STOP OFF and UT keys Figure 5 14 Keypad and display of the Multiskan GO Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 27 Instrument User Interface Display and keys lt a VP 2 3 abc def 4 5 6 ghi jkl mno pgrs tuv WXYZ BEG ES EA EA 28 Thermo Scientific Multiskan GO User Manual The user interface keys are detailed below The LEFT RIGHT UP and DOWN arrow keys navigate the menu structure move inside windows and change numeric values Repeat a selection by holding down the arrow key The OK key selects and edits the highlighted item The alphanumeric keys enter numerical data and text e Press a key several times to cycle through the characters e Pressing the 0 key enters a character space in text fields e Period Dot decimal point The CLEAR C key deletes text and numbers The F1 F3 keys select the action shown on the info text bar above the corresponding key The info text bar is updated according to the active menu The FILE key opens the File menu for containing data management functions The HELP key offers information on the current view The IN OUT key moves the plate carrier into or out of the instrument The START ON key e Switches the instrument on e Starts a microplate or cuvette measurement e Accepts setti
2. 5 Press the F3 Close key to return to the Settings menu For more information on the Settings menu refer to Settings menu on page 48 20 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Installation setting the instrument clock Setting the The instrument date and time settings may be incorrect on delivery and instrument clock should be checked and set before using the instrument To set the instrument date and time lt j gt 1 Select the Settings menu tab using the arrow keys ra Select Date amp Time with the arrow keys and press the OK key Press the OK key to select Time Enter the correct date and time with the arrow keys and the alphanumeric keys and press the OK key to confirm the selection settings Date amp Time parameters 03 06 2010 10 08 11 Date format dd mm yyyy Time format 24 hour 5 Press the F3 Close key to return to the Settings menu In the Date amp Time submenu you can also select the display formats for the date and the time updates the instrument date and time according to the PC clock A Note Connecting the instrument to SkanIt Software automatically Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 21 Installation Power Save feature Power Save feature Installing Skanit Software for Multiskan GO 22 Thermo Scientific Multiskan GO User Manual The Multiskan GO has a Power Save feature which lowers
3. Thermo Scientific Multiskan GO User Manual Rev 1 0 Cat no N10588 Copyright Copyright 2010 Thermo Fisher Scientific Corporation All rights reserved Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks Multiskan and SkanIt are registered trademarks of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions Remarks on screenshots Screenshots may be slightly different on your system depending on the firmware version No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Power failure The system requires uninterrupted power supply in order to operate correctly Thermo Fisher Scientific has no responsibility whatsoever for system malfunctions arising from
4. dd mm yyyy dd mrm Time format yyyy mm dd mimidd yyyy In number or text fields you can enter characters with the UP and DOWN arrow keys or the alphanumeric keys settings Date amp Time parameters Time 14 04 2010 12 28 30 Date format dd mm yyyy Time format 24 hour Cancel Thermo Scientific Multiskan GO User Manual 29 Instrument User Interface Instrument incubator In each view of the user interface the info text bar shows the actions available for the F1 F3 keys L 22 3 C 30 C j Results The info text bar also displays the current instrument temperature as well as the target temperature when the incubator is activated If a printer or USB memory device is connected to the instrument a corresponding icon is displayed on the info text bar Note The Multiskan GO maintains the used settings and parameters until these are changed A Instrument The incubator temperature can be controlled from the Plate and Cuvette menus using the F1 key incubator The info text bar shows the current temperature in degrees Celsius C Depending on the selected menu either the plate measurement chamber or the cuvette station temperature is displayed Press the F1 key to set the instrument incubator temperature Cuvette Kk Settings Cancel The temperature setting range is 10 45 C with a resolution of 1 C When the heating is on and the temperature set the target temperature
5. 13 53 54 ordering information 3 69 P packing 15 60 61 82 Thermo Scientific Multiskan GO User Manual instructions 60 list 15 materials 61 photometric 10 assay 10 photometry 64 79 plate 9 10 12 13 14 17 19 23 25 28 29 30 31 32 33 34 37 38 40 48 51 52 53 54 55 56 57 58 59 60 63 64 79 measurement 13 30 32 38 51 menu 23 31 32 33 results 37 type 23 Power Save mode 9 22 48 49 50 63 79 precision 32 37 40 43 64 printer 14 18 30 38 39 48 63 printing 31 39 46 48 results 31 39 48 printing or exporting data 24 39 refitting the transport lock 58 60 remote control 22 28 S saving results 38 self diagnostics 19 23 79 service 15 19 53 54 56 58 60 67 69 Settings 20 21 22 29 48 49 52 menu 20 21 22 29 48 49 shaker 14 63 shaking 9 14 32 35 63 speed 14 35 shutdown 42 Single 32 35 36 37 39 40 42 46 single measurement 36 46 79 spare parts 69 specifications 3 63 general 63 performance 64 safety 61 65 Spectrum 32 36 37 39 40 42 43 46 speed 14 32 35 64 startup 23 52 switching on 23 symbols 4 Thermo Fisher Scientific system 2 5 10 60 71 79 configuration 49 log 60 T transport 15 16 17 18 57 58 59 60 63 69 package 15 57 troubleshooting 3 67 type label 4 53 U USB 14 18 30 38 39 45 46 47 48 51 58 63 69 79 memory devic
6. L Pathlength correction Yes No L Pathlength 0 1 10 0 mm Zeroing for cuvettes In order to perform measurements the instrument needs to initialize calibrate itself This process is called zeroing In microplate measurements the Multiskan GO performs zeroing automatically 40 Thermo Scientific Multiskan GO User Manual In cuvette measurements however the user must perform zeroing manually The instrument must be zeroed to determine the instrument zero absorbance level 0 000 Abs before the first measurement of each cuvette measurement type All measured sample absorbances will be measured in comparison to this baseline level The instrument s zeroing for cuvettes can be performed in several ways each having some benefits and pitfalls The recommended zeroing process is to run the zero measurement twice first with an empty cuvette port and then with an assay blank cuvette o First zero with the empty cuvette port to set the baseline level of the instrument to zero Thermo Fisher Scientific O Instrument User Interface Cuvette measurement Then place the assay blank cuvette into the cuvette port and measure the absorbance of the blank sample with a normal measurement This reveals possible contaminations or other interferences in the assay blank samples When the assay blank level is considered to be suitable with regard to the assay chemistry re zero the instrument using the blank cuvette e Alternatively perfor
7. The unique Power Save function lowers its energy consumption The Multiskan GO can be used in a variety of applications including nucleic acid and protein analysis ELISA assays enzyme assays cytotoxicity and cell proliferation assays as well as apoptosis assays The Multiskan GO is available in the following configurations e Multiskan GO w o cuvette 100 240 V Cat no 51119200 and 51119250 Japan 96 and 384 well plate reading shaking and incubation e Multiskan GO with cuvette 100 240 V Cat no 51119300 and 51119350 Japan 96 and 384 well plate reading shaking and incubation Cuvette reading capability with incubation Note This manual covers the operation of the Multiskan GO with the cuvette instrument version If you have the Multiskan GO without the cuvette you can skip over the parts of the manual referring to cuvette use A Thermo Scientific Multiskan GO User Manual 9 Introduction to the Multiskan GO Intended use Figure 1 1 Multiskan GO with cuvette port A and without cuvette port B Intended use The Multiskan GO is a spectrophotometer that is used to measure photometric assays with defined 96 and 384 well microplates The Multiskan GO with the cuvette version can also measure photometric assays with cuvettes The Multiskan GO is intended to be used in research laboratories by professional personnel The Multiskan GO is designed to be part of an analyzing system for the end user w
8. o 0 0 0 0 0 0 iE Lp OR 02 N 29 oo 99 oo OF o cn oO Nua 99 oo oF om un ain Na oo io a o wio BO UN o gt Plate Graph If a result table does not fit the view you can scroll the table with the arrow keys The icon at the top right of the view shows your current location in the table In multiple wavelength measurements the results are listed starting from the top for each well Spectral measurement results are displayed one well at a time You can move between wells with the arrow keys The identified measurement peaks for each well are marked on the graph and listed beside it You can scale the graph between the well and plate maximum values with the F2 Scale key Press the F3 Close key to close the measurement results Press the F3 Results key to view the most recent measurement results if present Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 37 Instrument User Interface Plate measurement Saving results Exporting results 38 Thermo Scientific Multiskan GO User Manual Settings Session Session12 Plate format 96 wells All 1e gt l PON 00 00 00 Shaking No ncubation Reguired temp No amp Measurement 450 nm 620 nm F Results The asterisk by the session name indicates unsaved results These are retained until new results are generated To store results for later use you should save them in the instrument s memo
9. 2 Use disposable gloves 3 Clean the plate carrier with a soft cloth dampened with water or mild detergent 4 Ifyou have spilt infectious agents on the plate carrier decontaminate the instrument Refer to Decontamination procedure on page 56 Warning Ensure that the bottom of each microplate is dry Fluid on the bottom of a microplate may present a contamination hazard Use good laboratory practices when handling any hazardous materials A To clean the cuvette port follow these instructions Clean the cuvette port with a Q tip cotton swab moisturized with water mild detergent or 70 ethanol Thermo Scientific Multiskan GO User Manual 55 Maintenance Disposal of materials A Instrument clock battery Disposal of materials Decontamination procedure A 56 Thermo Scientific Multiskan GO User Manual Caution Do not clean the cuvette port Figure 2 2 if a cuvette has broken If the cuvette port becomes completely contaminated contact authorized technical service A The instrument electronics contains a battery for keeping the date and time information The battery is only in use when the power supply is disconnected The typical lifetime of the battery is 2 5 years If the instrument starts loosing its date and time information call service Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Refer to local regulations for the disposal of infectious mater
10. There is sufficiently room behind the instrument to enable disconnecting the device The ambient air is clean and free of corrosive vapors smoke and dust The ambient temperature range is between 10 C and 40 C The humidity is low so that condensation does not occur relative humidity is between 10 and 80 Caution Do not operate the instrument in an environment where potentially damaging liquids or gases are present A Installation steps This section describes the installation steps that have to be carried out before operating the instrument installing and switching it on to prevent condensation causing a short Caution Leave the instrument to sit for at least three hours before circuit A Removing the To remove the transport lock transport lock Caution Do not attempt to operate the instrument with the transport lock in place Figure 3 7 A Figure 3 7 Multiskan GO with the transport lock in place l 16 Thermo Scientific Multiskan GO User Manual Open the measurement chamber door by pushing the bottom edge and pulling the upper edge Figure 3 8 Thermo Fisher Scientific Installation Installation steps e Figure 3 8 ransport lock bar with warning tag 2 Unscrew the transport lock bar by turning it counterclockwise Use a suitable flat head screwdriver if necessary 3 Pull the plate carrier until it is fully out of the instrument Figure 3 9 Fixing screw Fig
11. a new cuvette measurement is run If you want to save the cuvette results transfer them immediately to a USB memory device after the measurement Thermo Scientific Multiskan GO User Manual 45 Instrument User Interface Cuvette measurement 46 Thermo Scientific Multiskan GO User Manual The presentation of the results depends on the session Single wavelength sessions the latest result is presented at the top and the measurement history is listed below it in three columns An entry of 0 0000 Z indicates instrument zeroing Cuvette Single Latest 0 3107 The measurement time is displayed to the right of each result Up to 24 measurement results are simultaneously displayed on the screen However the instrument stores up to 100 latest measurements which you can retrieve by exporting the single measurement to the USB memory device or by printing the results Press the C key to clear the result history list Spectrum sessions the measured spectrum is displayed with up to five identified peak values Cuvette Spectrum HAMA AL AN 00 300 400 500 600 700 800 900 1000 nm DNA RNA sessions the measured spectrum is displayed along with measured and calculated results Cuvette Nucleotide dsDNA A260 A3205 A280 A3205 50 A260 25 8 pg ml Thermo Fisher Scientific Thermo Fisher Scientific Exporting results sport new file Export append Print Instrume
12. and the F1 Accept or the OK key to select the session Nucleotide dsDNA fA2ZEO AS2O gt CAPSo Ageos 1 20 50 A260 6 83 pg ml You can print measurement results directly to a connected USB printer In addition to session and run information the printed report also includes the print header if it has been defined in the settings The Settings menu allows you to configure instrument operation The settings are universal for the instrument and do not differ between plate and cuvette use The values shown in the Settings menu are stored in the instrument memory and are not session specific Cuvette Settings amp Configuration Date amp Time Print header status report The Settings menu contains the following parameters e Configuration Select the user interface language Power Save setting and export decimal delimiter or reset the instrument to factory default values e Date amp Time Set the instrument date and time and select the date and time formats e Print header Enable or disable the header for printed reports and enter the header text e Status report View export and print the instrument status report Thermo Fisher Scientific Configuration Thermo Fisher Scientific Instrument User Interface settings menu A detailed overview of the Settings menu is shown in Table 5 7 Table 5 7 Settings menu parameters Settings menu Configur
13. category ll according to IEC 60664 1 see Note 1 overvoltage category Pollution degree 2 according to IEC 60664 1 see Note 2 Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 65 Technical Specifications Safety specifications 66 Thermo Scientific Multiskan GO User Manual Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation A EMC performance EN 61000 6 3 2007 Generic standards Emission standard for residential commercial and light industrial environments EN 61000 6 1 2007 Generic standards Immunity standard for residential commercial and light industrial environments EN 61326 1 2006 Product family standard Electrical equipment for measureme
14. direction arrow on the cuvette station Figure 4 13 Ensure that the cuvette is fully inserted Figure 4 13 Multiskan GO cuvette port 7 Press the START ON key to start the measurement 8 View the results of the measurement 9 Export or print the measurement data if desired 24 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific A Shutting down Thermo Fisher Scientific Routine Operation Shutting down Note Always keep the same cuvette orientation A Caution Ensure that you use a correct cuvette type with a measurement position height of 8 5 mm When you work in the UV range use quartz or other UV compatible cuvettes A To shut down the Multiskan GO instrument 1 Remove any plates or cuvettes from the instrument 2 Switch off the instrument after use by pressing and holding the STOP OFF key for a few seconds Thermo Scientific Multiskan GO User Manual 25 Routine Operation shutting down 26 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Chapter 5 Instrument User Interface This chapter describes the display and the keypad Figure 5 14 for navigating the menus and editing the parameters of the Multiskan GO instrument Display and keys Scien Tire MULTISKAN GO Menu Active row Session Session Plate format 96 wells Display Area All OO 00 00 Shaking Mo IAEUDANGn Required temp Mo Measurement 450 nm 620 nm F Info text bar f 22 00
15. is displayed on the info text bar see below 30 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific File menu Instrument help Thermo Fisher Scientific Instrument User Interface Instrument incubator Cuvette Settings Single Spectrum DNA RNA You can toggle the instrument incubator on and off with the F1 key When you turn off the heating the heating icon blinks for a few seconds Note The Plate incubation setting Required temp does not affect the real instrument temperature but is only used as a session specific temperature reminder for the user A The File menu provides you with data management functions such as exporting and printing results or storing session data Press the FILE key to open the File menu in the Plate or Cuvette menu Use the arrow keys to highlight the desired menu function and press OK to select it In the Plate menu you can open save and delete sessions from instrument memory as well as export and print measurement results In the Cuvette menu you can export and print results but not save session parameters or results in the internal memory The instrument Help feature presents information on the menu items in the current view Press the HELP key to open the help window and use the arrow keys to scroll the view if necessary Press the OK key to close the help window and return to the previous view Thermo Scientific Multiskan GO User Manual 31 Inst
16. number User Date Comments PHOTOCOPIABLE Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 71 System Log 12 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Appendix B Certificate of Decontamination Name Address Tel Fax Instrument Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE Please include decontaminating solution used Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 73 Certificate of Decontamination 74 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Appendix C Example of Available Cuvettes These cuvette lists are given only as an example Table 9 14 and Table 9 15 There are many other cuvettes available t
17. power consumption when the instrument is idle You can control the feature in the Settings menu where you can set the idle period before activation or turn the feature off In the power saving mode the stepper motors and incubators are shut down the flash lamp is off and the display is dimmed The state of the user interface is stored To reactivate the instrument press any instrument key The Skanlt Software reactivates the instrument automatically The user interface returns to the previous state To change the Power Save parameter 6 Select the Settings menu tab using the arrow keys 7 Select Configuration with the arrow keys 8 Select Power Save and press the OK key to change the parameter Select No to inactivate the Power Save feature For information on installing SkanIt Software for Multiskan GO refer to the SkanIt Software for Multiskan GO User Manual Cat no N10597 When the instrument is connected to SkanIt Software the Remote mode active text is shown on the display The STOP OFF key on the keypad can be used to disconnect the computer remote control Thermo Fisher Scientific Switching on Chapter 4 Routine Operation Before switching the instrument on check that all the cables are 8 properly fitted according to the installation instructions Switch the instrument on by pressing the START ON key The instrument proceeds to perform the self diagnostics and displays Self diagnostics pass
18. 0 21 SO0G 10 29B 1 10 29B 0 10 29B S0G 10 16 10 0 10 2Z8 5 16 R 10 Q 10 28 5 16 40 0 10 8 5 16 R 40 0 10 8 5 16 50 0 10 Z8 5 16 100 0 10 Z8 5 16 160 0 10 Z8 5 18 1 10 18 0 10 18 506 10 18B 1 10 18B 0 10 18B 506 10 28 1 10 28 0 10 28 506 10 28B 1 10 28B 0 10 28B 50G 10 Pathlength mm 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 Height mm 48 00 48 00 45 00 45 00 48 00 48 00 48 00 45 00 45 00 45 00 45 00 45 00 45 00 Width mm 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 12 50 Example of Available Cuvettes Depth mm 12 50 12 50 12 50 12 50 12 50 12 50 12 50 1250 12 50 12 50 12 50 12 50 12 50 Volume pl 800 400 3900 2500 2500 3500 1400 59 65 115 175 700 Meas position height mm 8 5 8 5 8 5 8 5 8 5 4 40 Thermo Scientific Multiskan GO User Manual 77 Example of Available Cuvettes Manufacturer Brand http www brandtech com Kartell http www kartell it uk labware w dis po htm Sarstedt http www sarstedt com php main php GE Healthcare http www gehealthcare com worldwide htm 18 Thermo Scientific Multiskan GO User Manual Table 9 15 Disposable plastic cuvettes Code s 159070D 159071D 159080 159081D 129170 159075D 159076D 159085D 75908
19. 12 50 12 50 5 8 5 105210 1085 40 10 00 40 00 12 50 12 50 10 8 5 107 100 10 40 10 00 06 00 12 50 12 50 3900 2 50 107 104 10 40 10 00 56 00 12 50 12 50 1400 2 50 Spectrocell R 0010 10 00 45 00 12 50 12 50 3500 2 40 R 2010 R 3010 R 4010 R 2010 T 10 00 00 00 12 50 12 50 3900 2 38 R 3010 T R 4010 T R 0010 S 10 00 57 00 12 50 12 50 3900 2 40 R 2010 S R 3010 S R 4010 S SM 0010 10 00 45 00 12 50 12 50 1400 4 40 SM 2010 SM 3010 SM 4010 SM 3010 B SM 4010 B ML 0010 10 00 45 00 12 50 12 50 600 4 40 ML 2010 ML 3010 ML 4010 ML 3010 B ML 4010 B GE Healthcare 80 2003 83 1 00 45 00 12 50 12 50 200 NA 80 2002 54 http www gehealthcare com 80 2003 85 5 00 45 00 12 50 12 50 1000 NA worldwide htm 80 2002 57 80 2003 87 10 00 45 00 12 50 al 2000 NA 80 2002 58 80 2099 89 80 2109 80 80 2109 81 80 2002 77 10 00 45 00 12 50 12 50 800 4 40 80 2004 15 80 2100 13 80 2109 82 80 2002 95 10 00 45 00 12 50 12 50 400 2 40 80 2100 25 80 2109 83 80 2002 70 10 00 48 00 12 50 12 50 2000 NA http www spectrocell com Continued 76 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Manufacturer GE Healthcare Cont Starna http www starna com Thermo Fisher Scientific Codels 80 2003 98 80 2002 81 80 2100 22 80 2002 99 1 6 10 1 1 10 1 0 10 1 506 10 9 1 10 9 0 10 9 506G 10 9B 1 10 9B 0 10 9B SOG 10 29 1 10 29 0 10 29 506 10 21 G 10 21 1 10 21 0 1
20. 48 G glossary 79 H header 14 39 48 49 50 51 52 heating 13 30 31 element 13 Help 28 31 67 how to decontaminate the instrument 53 55 56 57 60 73 how to install the Multiskan GO 15 16 19 65 how to maintain the instrument 53 how to switch on 23 importing data 52 incubation 9 13 31 32 35 parameters 32 35 incubator 13 14 30 31 39 64 info text bar 28 30 installation 3 15 16 22 23 65 66 category 65 66 intended use 3 10 internal memory 31 Thermo Scientific Multiskan GO User Manual 81 K keypad 22 27 54 63 keys 19 20 21 22 23 24 25 28 29 30 31 33 34 35 37 38 41 46 47 48 58 kinetic 9 39 40 45 47 79 measurements 9 45 47 79 session 45 47 L lamp 22 63 layout 34 linear shaking 63 low volume plates 12 maintaining a system log 53 60 maintenance 3 14 53 54 60 checklist 53 measurement 3 12 13 14 16 17 19 23 24 25 28 29 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 50 52 55 57 58 59 60 63 64 66 79 chamber 16 17 19 55 57 58 59 60 chamber door 16 17 19 57 58 59 60 direction 12 24 mode 37 42 43 parameters 23 29 32 36 39 speed 37 43 64 technique 64 time 46 measuring 23 24 79 a cuvette 24 a plate 23 memory device 14 63 79 menu 20 28 29 30 31 33 38 40 navigating 3 27 0 optical 13 41 53 54 79 system
21. 6D 7 59165 159150 7 59152 159210 159200 159219 159218 1937 1938 67 742 67 746 67 740 67 741 67 745 67 738 80 2004 53 80 2084 11 80 3000 77 Pathlength mm 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 10 00 Height mm 45 00 45 00 45 00 45 00 45 00 45 00 45 00 Width mm 12 50 12 50 12 50 12 50 12 50 12 50 12 50 Depth mm 12 50 12 50 12 50 12 50 12 50 12 50 12 50 Volume ul 4500 3000 850 4500 1500 1500 4500 4500 2500 Meas position height mm 4 40 4 20 8 5 4 40 4 40 4 40 4 40 NA NA NA Thermo Fisher Scientific Glossary absorbance optical density A logarithmic function of the transmission of a wavelength of light through a liquid log b dimension Ads decontamination Removal or neutralization of radiologic bacteriological chemical or other contamination disinfection The destruction of pathogenic bacteria usually with an antiseptic chemical or disinfectant dynamic range The range of signals an instrument can read from the minimum to the maximum detectable For example a dynamic range of three decades means that the difference between the lowest and highest signals that can be measured is 10 EIA Enzyme immunoassay An immunoassay using a color changing enzyme substrate system for indicating results A diagnostic test method to m
22. ATION AVANT L ENTRETIEN ET LE DEPANNAGE 4 Thermo Fisher Scientific About This User Manual safety symbols and markings Warning and other The following symbols and markings appear in this user manual markings used in the documentation Warning Risk of electric shock A Warning Biohazard risk A Warning Risk of injury to the user s A Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application A Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest A DE RDS Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 5 About This User Manual safety symbols and markings 6 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Thermo Fisher Scientific Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Contents Inten WISE G tails aera sae en BN NB na 3 How to use this user manual So an ab aa 3 Thermo Scientific SkanIt Software oooooWooooocm 3 For more information oo oooooWoWoW oo Wen 4 Salety symbols aad marking ea Ran tata ieee 4 Introduction to the Multiskan GO 9 Inter eU ne a nee BEE 10 General DESCnipuon kiasan 11 NF ako lus aa cig tan ayn ap enter na 11 Plite Ci cheers EN EE E cates tea 12 Cavento POr aae ET E An 12 Optical System benua 13 Na Opi O PN AA EP PON ANE PA AT NAN 13 NN Kel ane AA ANA T E NN AAA eT AA An 14 USB port
23. GO User Manual 61 Maintenance Disposal of the instrument 62 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific General specifications Thermo Fisher Scientific Chapter 7 Technical Specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program Table 7 9 through Table 7 11 Table 7 9 General specifications General specifications Overall dimensions Weight Operating conditions Transportation conditions Storage conditions Mains power supply Power consumption Heat dissipation User interfaces USB connections 2 0 compatible Light source Detectors Wavelength selection shaker Plates Cuvettes 285 mm W x 430 mm D x 260 mm H 11 2 W x 16 9 D x 10 2 H 10 8 kg 23 8 Ibs 10 C to 40 C maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Indoor use only 40 C to 70 C packed in transport packaging 25 C to 50 C packed in transport packaging 100 240 Vac 50 60 Hz Max 110 W typical operation lt 22 W Power Save lt 2 5 W 375 BTU max Standalone use 4 5 color display and keypad PC control Skanit Software PC Memory device port for data export External printer HP PCL5 compatible Xenon flash lamp Photodiodes for measurement and reference Monochromator Linear shaking with a
24. O User Manual 49 Instrument User Interface settings menu For more information on Power Save refer to Power Save feature on page 22 e Export decimal delimiter Select the decimal separator Dot or Comma This setting defines the decimal delimiter only for exported measurement results It does not affect the displayed or printed results which always use the decimal point e Reset to factory defaults Reset the instrument to its factory default values Date amp Time In the Date amp Time parameters submenu you can set the instrument date and time and the date and time formats You should set the correct time before you put the device into use Once the time is set the instrument clock will keep running even when the instrument power is switched off When you connect the Multiskan GO to Skanlt Software the instrument date and time is set automatically if Settings y 12 20 BN Date amp Time parameters 14 04 2010 1 2 26 30 Date format dd mm yyyy Time format 24 hour Cancel The Date amp Time parameters submenu contains the following parameters e Time Set the instrument date and time e Date format Select the date format dd mm yyyy dd mm yyyy dd mm yyyy yyyy mm dd or mm dd yyyy e Time format Select the 12 hour or 24 hour time format Print header You can also include the print header in the printed report and export file if it has been defined in the settings 50
25. TXT e Instrument status reports REP TXT All files of the same type are numbered sequentially PLA00001 TXT PLA00002 TXT and so on Thermo Scientific Multiskan GO User Manual 51 Instrument User Interface Export file formats 52 Thermo Scientific Multiskan GO User Manual The exported files are in ASCII format and can be opened with any text editor such as Microsoft Notepad Result files can be imported into Microsoft Excel The measurement results are exported using four decimal places Note Set the export decimal delimiter to the desired value in Settings Configuration A Plate sessions saved in the instrument memory can be transferred to SkanIt Software Cuvette data cannot be transferred to SkanIt Software The checksum at the end of exported files is used by SkanIt Software when importing data Plate and cuvette export files contain the following information e Print header e Measurement session information e Instrument information e Session start and stop times e Temperatures at the beginning and end of the measurement e Measurement results File Edit Format View Help This is the header Session13 PLA 1 10 05 2010 10 11 15 Session Session Session1l3 Plate format 96 wells Measurement type Multiple Measurement mode t wavelength 1 nm wavelength 2 nm ta Ab 3 nm i temp C aking 3 Interval Duration Chh mm ss 00 00 00 Speed 1 Medium Instrument Multiskan GO 1 00 17 Seri
26. Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Thermo Fisher Scientific Instrument User Interface Export file formats settings Print header Enable header Yes Header text Lab info 220c The Print header submenu contains the following parameters e Enable header Enable or disable the print header If activated the header is used for all printouts and is displayed at the top of each page e Header text Enter a header text A customized print header can contain for example laboratory information The maximum length of the header is approximately 50 characters Status report You can view export and print the instrument s status report Export file formats Settings 10 09 INSTRUMENT STATUS REPORT 03 06 2010 10 09 18 Instrument Name Multiskan GO Cuvette Yes Serial number 1510 00000C FW version 1 00 24 Startup tests Plate movement NOT TESTED Measure head movement PASSED The status report provides current information on the instrument status including the instrument model and serial number firmware version current temperature and other parameters When exporting data to an external USB memory device the Multiskan GO stores the files in a folder named GO The folder is created automatically if it does not already exist The Multiskan GO can export three types of files e Plate measurement results PLA TXT Cuvette measurement results CUV
27. al number 1510 00000c Start time 10 05 2010 10 10 30 Stop time 10 05 2010 10 10 30 Start temperature 22 0 Stop temperature 22 0 450 nm 1 2 3 4 5 6 7 0 3513 1 1510 0 0815 7 0 0481 0 2128 0 0418 5 6 0 1793 0 8446 0 0327 1 2412 0 8879 0 0445 0 3363 z 0 1993 0 1980 0 0657 0 1380 0 1867 0 7012 0 7681 0 4819 0 5749 0 3260 0 4026 0 1226 0 3991 0 2830 0 4636 0 0355 0 2733 0 16 0 5020 The instrument status report contains the following information e Print header e Instrument information name serial number and firmware version e Startup test results e Memory usage e Operating statistics e Temperature information e Detailed technical instrument data e Error log Thermo Fisher Scientific Chapter 6 Maintenance Regular ond Contact local authorized technical service or your local Thermo Fisher Scientific representative for assistance if necessary preventive maintenance Maintenance This chapter contains an outline of the points mentioned in the checklist checklist below Table 6 8 Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied Table 6 8 Maintenance checklist Item Weekly Monthly Yearly Keep the instrument free of dust See Instrument care on page 53 Avoid disturbing any of the optical system components including optical covers See Instrument care on pa
28. anual Ambient 4 C to 45 C Mean temperature of wells 1 5 C across the plate at 37 C 96 well plate Thermo Fisher Scientific Technical Specifications Safety specifications Safety This section describes the safety specifications for the Multiskan GO specifications In conformity with Multiskan GO bears the following markings the requirements Type 1510 24VDC 4A CE mark cTUV monogram Multiskan GO conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B July 2004 2002 96 EC WEEE Directive Waste of Electrical and Electronic Equipment 2002 95 EC RoHS Directive Restriction of the Use of Certain Hazardous Substances in Electrical and Electronic Equipment 2006 42 EC Machinery Directive Safety performance IEC 61010 1 2001 Ed 2 including US and CA National differences IEC 61010 2 010 2003 Ed 2 IEC 61010 2 101 2002 Ed 1 Particular Requirements for In Vitro Diagnostic IVD Medical Equipment The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude Up to 2000 m Temperature 5 C to 40 C Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains supply fluctuations 10 from nominal Installation
29. asurement chamber door by pushing it from the bottom edge and pulling it from the top edge 4 Pull the plate carrier fully out of the instrument Figure 6 17 Figure 6 17 Plate carrier fully out of the instrument 5 Take the transport lock bar from the back of the instrument and locate it into the slot on the plate carrier and then fasten the red fixing screw Figure 6 18 58 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Maintenance Refitting the transport lock Tn S s Ee 5 Figure 6 18 Iransport lock bar in place on plate carrier 6 Push the plate carrier fully into the measurement chamber with the transport lock bar and screw the rod into locked position 7 Close the measurement chamber door and make sure that the end of the transport lock bar enters the corresponding recess in the door and that the warning tag hangs out of the instrument Figure 6 19 Figure 6 19 Multiskan GO with the transport lock in place Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 59 Maintenance Packing for service Packing for service Maintaining a system log Disposal of the instrument 60 Thermo Scientific Multiskan GO User Manual To pack the Multiskan GO for service follow the instructions presented below e Inform about the use of hazardous materials e Remove any microplate or cuvette before decontamination Decontaminate the instrument e Refi
30. ate measurements but manually for cuvette measurements Refer to Zeroing for cuvettes on page 40 Thermo Scientific Multiskan GO User Manual 79 Glossary 80 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Index A absorbance 9 12 40 41 42 64 79 accessories 15 69 accuracy 2 43 54 64 append 48 application 2 5 9 10 autocalibration 79 bandwidth 64 Brief Users Guide 3 69 C calculated results 46 changing the language 20 49 connecting to a computer 18 connecting to a printer 18 cuvette 3 9 10 11 12 13 14 24 25 28 29 30 31 39 40 41 42 43 44 45 47 48 49 51 52 53 55 56 57 60 63 64 69 75 79 glass or quartz 75 measurement 9 28 29 39 40 42 45 51 79 menu 24 29 30 31 39 40 plastic 75 78 port 10 12 13 14 24 40 41 53 55 56 57 60 port cover 24 results 45 sessions 39 45 47 48 station 24 30 41 49 D Date amp Time 21 33 48 49 50 56 decontamination 53 55 56 57 60 73 79 Certificate of 57 60 73 procedure 53 55 56 57 60 73 disposal of instrument 60 disposal of materials 56 DNA RNA 39 40 43 44 46 Thermo Fisher Scientific E endpoint measurement 36 46 79 environmental requirements 15 error 19 52 60 67 codes 67 messages 19 60 export file formats 38 47 51 exporting results 24 31 38 39 47 48 49 50 51 52 63 F File 28 31 38 47
31. ation L Language English Chinese French German Japanese Portuguese Russian Spanish L Power save No 5 15 30 60 L Export decimal delimiter Dot Comma L Reset to factory defaults Yes No Date amp Time LTime L Date format dd mm yyyy dd mm yyyy dd mm yyyy yyyy mm dd mm dd yyy L Time format 12 hour 24 hour Print header L Enable header Yes No L Header text Status report L Print L Export The Configuration parameters submenu allows you to select the user interface language Power Save setting and the export decimal delimiter You can also reset the instrument to its factory default values The submenu also displays the instrument s firmware version serial number and whether it is equipped with a cuvette station f Settings Jo a CPX Chinese Configuration Francais French Deutsch German B Japanese Ca Multiskan GO Portugu s Portuguese Power save Pycckuh Russian Firmware 1 00 12 Enact Espa ol Spanish SN 0 1510 00000C decimal delimiter Cuvette Yes Reset to factory defaults The Settings configuration window contains the following parameters e Language Select the user interface language English Chinese French German Japanese Portuguese Russian or Spanish To change the user interface language refer to Changing the language on page 20 e Power Save Select the Power Save setting No 5 min 15 min 30 min or 60 min Thermo Scientific Multiskan G
32. ation cable to the USB port marked USB PC Figure 3 11 If you are using a printer connect it to the USB port marked USB PRINTER Figure 3 11 This instrument is designed to be safe for use When correctly installed operated and maintained it will present no hazard to the user Thermo Fisher Scientific Electrical A A Operational Thermo Fisher Scientific check Installation Operational check The following recommendations are given for added user safety Ensure that the power supply cable supplied with the unit is always used If a correct type of mains cable is not provided use only cables certified by the local authorities The power plug should only be inserted into a socket outlet with a rotective ground contact Never use an extension cable without a g rotective ground wire g Warning Only authorized technical service personnel are allowed to open the instrument Disconnect the instrument from all voltage sources by disconnecting the power supply cable before opening it A Warning Do not touch switches or electrical outlets with wet hands Switch the instrument off before disconnecting it from the mains supply 4 Before you put the instrument into use perform the following operational check 1 Switch the instrument on by pressing the START ON key The instrument proceeds to perform the self diagnostics 2 Check that the plate carrier comes out of the instrument and the Self diagnosti
33. cept Cancel L 22 0 C Accept Cancel single Multiple Spectrum e Step Only used in Spectrum measurements The measurement step range is 1 800 nm in increments of 1 nm e Mode Select the measurement mode Fast or Precision The default Fast mode is a measurement speed optimized mode that produces results that are sufficiently precise for most uses The Precision mode takes more time to perform a measurement when the best possible measurement precision is required Plate results After the instrument has completed a measurement it automatically displays the results In single and multiple wavelength measurements the results are displayed as a table while spectral scanning measurements are displayed as a graph Cuvette Spectrum N O Ox D NN o on oo i on ag I oo 90 Ag Hg ao o 00 no ng om o an ion ue cow a iy un al UT ca On N in an al p a un N tu o N iy oo a o o o D in N a ot 5 D a alo up pO wu iv o on 99 99 99 an BA NG move See a A in ON N P Os eN O N BO RU N aa AN aa aN Ny NS NO N Su Ou ou t a po woul o oo 99 99 on am BO WO ON AN wo 00 00 OO oo o oO oun No BO or Ny o z0 99 oo oo NG do o o 29 ae om N NO NG ay oo UN ao E a 00 00 or gt WO N ooo os o ay al oO a N a N 99 99 op u o amp o 0 0 0 Oo
34. cs passed text appears briefly on the display 3 Press the START ON key 4 Check that the plate carrier moves in and the measurement chamber door closes properly The instrument starts the measurement After the measurement the plate carrier comes out and the measurement results are displayed No error messages should appear on the display 5 Press the F3 Close key to close the result table Thermo Scientific Multiskan GO User Manual 19 Installation Changing the language Changing the You can select the preferred language for the user interface The user anguage interface errors and warnings printouts and exported data are available guag in the selected language To change the user interface language a gt 1 Select the Settings menu tab using the arrow keys A F oK 2 Select Configuration with the arrow keys and press the OK key 3 Press the OK key to select Language PX Chinese Configuration Francais French Deutsch German B Japanese Portugu s Portuguese ag a Power save Pyccknit Russian Firmware 10012 Bammer ee SN 1510 000000 SEN P3 Ag decimal delimiter Cuvette Yes Reset to factory defaults Cancel 4 Choose the user interface language from the list using the arrow keys and press the OK key to confirm the selection The available languages are English default German French Spanish Portuguese Russian Chinese and Japanese AVG
35. de and its respective language versions o Skanit Software for Multiskan GO User Manual o Skanit Software for Multiskan GO Brief Users Guide and its respective language versions Thermo Scientific Multiskan GO User Manual 3 About This User Manual For more information For more information Safety symbols and markings Safety symbols and markings used on the Multiskan GO Bc Gal leh O 4 Thermo Scientific Multiskan GO User Manual For the latest information on products and services visit our website http www thermoscientific com http www thermoscientific com mpi http www thermoscientific com readingroom In our efforts to provide useful and appropriate documentation we would appreciate any comments you may have on this user manual to your local Thermo Fisher Scientific representative These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards The following symbols and markings appear on the type label and the instrument itself Power on A Power off a Serial number a Catalog number a Date of manufacture a Consult instructions for use a WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC A A black label with the following text Figure 2 3 CAUTION WARNING DISCONNECT SUPPLY BEFORE SERVICING and AVERTISSEMENT COUPER L ALIMENT
36. djustable speeds The cuvette option does not support shaking 96 and 384 well formats Maximum plate height with lid 18 5 mm Dimensions 12 5 W x 12 5 D x 40 58 H mm Beam center height 8 5 mm Thermo Scientific Multiskan GO User Manual 63 Technical Specifications Performance specifications Performance This section provides the performance specifications for the relevant specifications measurement technique and other instrument capabilities Table 7 10 Photometry Performance specifications Photometry Wavelength range Wavelength resolution Wavelength accuracy Wavelength repeatability Bandwidth Read out range Linearity 450 nm Accuracy 450 nm Precision 450 nm stray light Measurement speed from A1 back to A1 Spectral scanning speed 200 1000 nm 1 nm 2 nm 0 2 nm lt 2 5 nm Up to 4 Abs Plate Cuvette 0 2 5 Abs 2 96 well plate 0 2 5 Abs 2 1 0 0 003 Abs 0 2 0 Abs 1 0 0 003 Abs 0 2 0 Abs 2 0 2 0 2 5 Abs 2 0 2 0 2 5 Abs SD lt 0 003 Abs or CV lt 0 5 SD lt 0 003 Abs or CV lt 0 5 Precision mode SD lt 0 003 Abs or CV lt 1 0 Fast mode 0 05 230 nm 96 well plate Fast mode 6 s Precision mode 27 s 384 well plate Fast mode 10 s Precision mode 92 s 10 s from 200 to 1000 nm with 1 nm steps Table 7 11 Incubator Performance specifications Incubator Temperature range Uniformity 64 Thermo Scientific Multiskan GO User M
37. e 14 30 38 39 45 46 47 48 51 79 User formula 39 40 44 47 user interface 3 20 22 28 29 30 33 42 48 49 63 Thermo Fisher Scientific V viewing results 24 W warning codes 67 warranty 15 wavelength 9 32 33 36 37 40 42 43 44 45 46 63 64 79 accuracy 64 range 9 36 64 repeatability 64 resolution 64 Z zero 24 36 40 41 42 zeroing 24 36 40 41 42 46 79 Thermo Scientific Multiskan GO User Manual 83 Notes 84 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 85 Thermo Fisher Scientific Oy Ratastie 2 PO Box 100 F1 01621 Vantaa Finland www thermoscientific com N10588
38. e slit Fiberoptic link Exit slit Xenon flash light source Fiberoptic link e m Detector Cuvette reading station Rotating holographic grating SCmeasOptics dsf 8 12 2009 JTu Figure 2 6 Multiskan GO with cuvette optical system Incubator Both the plate carrier and the cuvette port have their own incubator The incubation temperature setting is the same for both the plate carrier and the cuvette port The plate measurement chamber incubator consists of thermal sensor controlled incubator zones with two at the top and two on the bottom of the incubator chamber The incubator design prevents condensation forming on the microplate lid The cuvette port incubator is equipped with a separate heating element controlled by a thermal sensor The incubation temperature range is from ambient 4 C to 45 C Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 13 General Description shaker Cooling fan The cooling fan is used to maintain the instrument temperature near ambient conditions It is automatically activated when the instrument measurement is ongoing The fan can also be activated for faster instrument cooling by setting the target temperature lower than the current plate incubator temperature The lowest possible temperature is slightly above ambient Shaker The linear shaker of the plate carrier operates at different adjustable speeds Table 2 2 The cuvette port does not support shaki
39. e to start a cuvette measurement without a valid zero value e The user interface shows the status of the zeroing e There are separate zero values for each measurement type The zeroing is valid only if it has been performed with the same parameters as during the session e The zeroing values are valid for ten hours or until the measurement mode or parameters are changed e The zeroing values are reset at shutdown Single wavelength Use the Single session to perform a single wavelength measurement on a cuvette Cuvette i 1247 Single parameters Wavelength nm Pathlength correction Pathlength mm Cancel The Single submenu contains the following parameters e Wavelength Enter the measurement wavelength The available range is 200 1000 nm e Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength e Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled Spectrum Use the Spectrum session to perform a spectral scanning measurement on a cuvette 42 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Thermo Fisher Scientific Instrument User Interface Cuvette measurement Cuvette Spectrum parameters SI Eau UNO gni Mode End wavelength nm 1000 step nm 2 Pathlength correction No Cancel T
40. easure or detect a substance using antibody antigen reactions ELISA Abbreviation for enzyme linked immunosorbent assay kinetic measurement Measurement over a time period at certain intervals memory device A USB memory stick to store results for later reference The results are saved in the instrument s memory and exported to the connected USB memory device monochromator Transmits certain wavelengths of the incoming light Consists of a rotatable diffraction grating and entrance exit slits optical density absorbance log 1 transmittance log h dimension O D photometer A device measuring absorbance or optical density photometry The measurement of the properties of light particularly luminous intensity Thermo Fisher Scientific power saving mode The instrument has a Power Save feature which lowers power consumption while the instrument is idle Refer to Power Save feature on page 22 and Configuration on page 49 self diagnostics Initialization tests and adjustments that the instrument performs before operation as well as autocalibration single measurement Synonym to endpoint measurement assay method The measurement is only done with a single reading transmittance The ratio of transmitted and incident light 4 Wb zeroing To perform measurements the instrument needs to initialize calibrate itself The process is called zeroing and is automatically performed for micropl
41. ed after it has been passed The instrument is now ready for use Measuring To measure a microplate Thermo Fisher Scientific plate 1 Load the microplate If the plate carrier is in first drive the plate carrier out by pressing the IN OUT key Always insert the microplate so that the Al corner is positioned in the top left hand corner of the plate carrier Figure 4 12 Figure 4 12 Multiskan GO with plate carrier out Caution Ensure that you select the correct plate type An unsuitable plate may become jammed in the instrument When you work in the UV range use quartz or other UV compatible microplates A 2 Define the measurement parameters in the Plate menu 3 Press the START ON key to start the measurement Thermo Scientific Multiskan GO User Manual 23 Routine Operation Measuring a cuvette 4 View the results of the measurement 5 Save export or print the results if desired Measu ring a To measure a cuvette cuvette 1 Open the cuvette port cover 2 Ensure that the cuvette port is empty or insert a cuvette with buffer to perform instrument zeroing 3 Close the cuvette port cover 4 Select the measurement session and define its parameters in the Cuvette menu 5 Zero the instrument by pressing the F2 Zero key For more information see Zeroing for cuvettes on page 40 6 Insert the sample cuvette into the cuvette port with its measurement windows aligned with the measurement
42. entific Multiskan GO User Manual 33 Instrument User Interface Plate measurement Table 5 5 384 well plate dimensions 384 well plate Dimension mm Plate length 85 48 Plate width 127 6 First well position X 12 13 First well location Y 8 99 Corner distance X 103 5 Corner distance Y 67 5 The maximum plate height with lid is 18 5 mm Measurement area Ifyou wish to measure only a certain part of the microplate you can define a measurement area To select a single rectangular Measurement area 1 Select the starting point with the arrow keys and press the OK key The OK key toggles between cursor movement and area selection The first point can be any point on the layout 2 Select the last point by highlighting the desired rectangular measurement area on the plate with the arrow keys Selected area ai Accept Cancel 3 Press the F1 Accept key to accept the area selection 4 You can toggle between A and None with the F3 Select all Clear key Press All if you want the entire plate to be measured or None to clear the plate 34 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument User Interface Plate measurement Incubation and You can define the incubation and shaking parameters in the shaking parameters Incubation parameters submenu Incubation parameters Duration hh mm ss 00 00 00 shaking Interval speed Medium Required temp C No L 22 0 C Cancel T
43. f and unplug the instrument 2 Use disposable gloves 3 Clean the instrument outside and the plate carrier with a soft cloth dampened with water or mild detergent Thermo Fisher Scientific A Cleaning the sample Thermo Fisher Scientific holder Plate carrier Cuvette port Maintenance Cleaning of the instrument 4 Ifyou have spilt infectious agents on the instrument decontaminate the instrument Refer to Decontamination procedure on page 56 Caution Do not use any solutions containing oxidative chemicals such as hypochlorites and peroxides or strong bases on any of the anodized aluminum surfaces plate carrier cuvette port as this may cause permanent damage to the finish A To clean the sample holder follow the instructions below To clean the plate carrier Caution Keep the instrument plate carrier clean to avoid dust and dirt from entering the measurement chamber Figure 2 2 Clean the plate carrier surface at least once a week using a soft cloth or tissue paper soaked in a mild detergent solution soap solution or 70 ethanol Wipe up spills immediately Do not use formaldehyde or strong alkalines A Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur A 1 Turn the power off and unplug the instrument
44. ge 53 Sl NS Daily Wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces immediately to prevent damage and wipe with deionized distilled water See Cleaning of the instrument on page 54 If any surfaces have been contaminated with biohazardous material disinfect with a mild sterilizing solution See Instrument care on page 53 Clean the case of the instrument periodically See Cleaning of the Instrument on page 54 Clean the plate carrier and cuvette port when necessary See Cleaning the sample holder on page 55 Decontaminate the instrument when relocating the instrument or sending it for service See Decontamination procedure on page 56 Service the instrument regularly See Cleaning of the instrument on page 54 and Maintaining a system log on page 60 vV depending on the laboratory conditions and the use and configuration of the instrument Instrument care Always ensure that the electricity supply in the laboratory conforms to that specified on the type label of the instrument Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 53 Maintenance Cleaning of the instrument DD gt Cleaning o instru CD gt D 54 Thermo Scientific Multiskan GO User Manual To guarantee the continuous reliability and accuracy of the Multiskan GO avoid disturbing any of the optical system components A misalignment of the light pa
45. hat can also be used with the Multiskan GO The Multiskan GO can be used with cuvettes that have external dimensions of 12 5 W x 12 5 L x 40 58 H mm and are compatible with a beam center height of 8 5 mm Table 9 14 Glass or quartz cuvettes Manufacturer Code s Pathlength Height Width Depth Volume Meas mm mm mm mm ul position height mm Hellma 105 810 UVS 1 00 03 00 12 50 12 50 0 8 5 100 10 20 10 00 45 00 1250 1250 3500 2 40 http www hellma worldwide com 100 10 40 100 10 46 6030 10 10 6030 UV 10 531 110 10 20 10 00 46 00 12 50 12 50 3500 2 40 110 10 40 110 10 46 6040 10 10 10 00 45 00 12 50 12 50 1400 4 40 6040 UV 10 531 104 10 20 104 10 40 104 10 46 104B 10 20 104B 10 40 104F 10 40 114 10 20 10 00 46 00 12 50 12 50 1400 4 40 114 10 40 114B 10 40 104 002 10 20 10 00 45 00 12 50 12 50 700 4 40 104 002 10 40 104 002B 10 20 104 002B 10 40 104 002F 10 40 115 10 40 10 00 40 00 12 50 12 50 400 2 39 115B 10 40 105 200 85 40 10 00 45 00 12 50 12 50 180 8 5 105 201 85 40 10 00 45 00 12 50 12 50 120 8 5 105 202 85 40 10 00 45 00 12 50 1250 10 8 5 105 203 1085 40 Continued Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 75 Example of Available Cuvettes Manufacturer Code s Pathlength Height Width Depth Volume Meas mm mm mm mm pl position height mm Hellma Cont 105 204 1085 40 10 00 45 00 12 50 12 50 40 8 5 105210 585 40 5 00 40 00
46. he Incubation parameters submenu contains the following parameters e Duration hh mm ss The duration is the period between pressing the START ON key to the beginning of the measurement The shaking principle is visualized differently according to the shaking ty Pe e Shaking You can specify the shaking mode e No Shaking is off e Single Shaking for 10 seconds before measurement e Double Two shaking periods one at the beginning of the incubation period and one right before the measurement Both shaking periods have a duration of 10 seconds Interval Shaking in intervals the On time is 10 s and the Off time 10 s The last period before measurement is always on You can set the duration for interval shaking e Speed You can define the shaking speed Low Medium or High e Required temp C You can add a target temperature for the session which is a temperature reminder for the user The Required temp parameter works as a reminder to use a certain temperature for a specific session If the target instrument temperature is different from the Required temp or if it has not been reached yet the instrument will give a warning to ensure whether the measurement shall be executed The user can select to measure or cancel the session Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 35 Instrument User Interface Plate measurement Settings temperature A Note The Required
47. he Spectrum submenu contains the following parameters Start wavelength Enter the start wavelength for the spectral scanning measurement The available range is 200 999 nm End wavelength Enter the end wavelength for the spectral scanning measurement The available range is 201 1000 nm The end wavelength must be greater than the start wavelength Step Enter the measurement step for the scan The available range is 1 800 nm in increments of 1 nm The default is the value used for the previous measurement The last step wavelength will be the last possible wavelength that is smaller than or equal to the end wavelength Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled Mode Select the measurement mode Fast or Precision The default fast mode is a measurement speed optimized mode that produces results that are sufficiently accurate for most uses The Precision mode takes more time to perform a measurement when the best possible measurement accuracy is required DNA RNA Use the DNA RNA session to perform a RNA dsDNA or ssDNA Measurement on a cuvette Cuvette DNA RNA parameters Nucleotide Pathlength correction Cancel Thermo Scientific Multiskan GO User Manual 43 Ins
48. ho is responsible for validating the system to ensure reliable and safe results We recommend using Good Laboratory Practices GLP during the analyzing process Use for self testing is excluded Caution Operate the instrument only with software and hardware that is specifically designed or selected for it Thermo Fisher Scientific assumes no liability for the use of third party software applications A 10 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Chapter 2 General Description This chapter describes the main parts of the Multiskan GO instrument Instrument The front view of the Multiskan GO with cuvette instrument is shown in Figure 2 2 views Cuvette port cuvette model Display ON key OFF key Keypad Plate measurement chamber door lt Figure 2 2 Multiskan GO with cuvette front view The rear view of the Multiskan GO instrument is shown in Figure 2 3 Power supply connector USB ports for printer Air inlet and memory device Type label Transport lock bar Storage position USB port for PC connection Figure 2 3 Multiskan GO rear view Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 11 General Description Plate carrier The side view of the Multiskan GO with cuvette instrument is shown in Figure 2 4 Plate carrier OUT Figure 2 4 Multiskan GO with cuvette side view Plate carrier The Multiskan GO supports all common 96 and 384 we
49. ial Warning The samples can be potentially infectious Dispose of all used disposable plastic plates and cuvettes syringes disposable tips disposable gloves and so on as biohazardous waste Be cautious and always use gloves A If you have spilt infectious agents carry out the decontamination procedure Warning The decontamination procedure should be performed by authorized and trained personnel in a well ventilated room wearing disposable gloves protective glasses and clothing A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another Example of decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 e Decon 90 min 4 Thermo Fisher Scientific Maintenance Decontamination procedure Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde as even small traces of formaldehyde negatively affect the enzyme being used in EIA tests resulting in bad test results A To decontaminate the instrument 1 Prepare the decontaminant 2 Empty the plate carrier and cuvette port Ensure that you are wearing disposable gloves 3 Switch off the power a
50. l Specifications oooooooooooo oo ooo oom momen 63 General Spec AON Sneak E NN 63 Performance Spec NA RON anna EN NN AN 64 Seret Spec WOL nana aa 65 Troubleshooting Guide oom 67 Handling abnormal situations ooooW WWW 67 Error and wamino code sn 67 Ordering Informati n anak knee maba 69 Viatuskan GO nenas east una 69 Listof spate parts and ACCESSOTLES sisi benakku Benua 69 System at Ka NN N N an etaeenaaees 71 Certificate of Decontamination ooooooooooooo oom 73 Example of Available Guvettes oooooooooo ooo os 75 Thermo Fisher Scientific Thermo Fisher Scientific Chapter 1 Introduction to the Multiskan GO The Multiskan GO Figure 1 1 is a high quality monochromator based UV VIS spectrophotometer It is used in spectral scanning end point and kinetic measurements to measure absorbance in the 200 1000 nm wavelength range from appropriate 96 or 384 well plates with and without lids and various types of cuvettes The instrument allows incubation up to 45 C and shaking of the microplate The instrument can be controlled as standalone with straight forward internal software for quick and simple plate and cuvette measurements or by PC using SkanIt Software It can be operated in several languages English German French Spanish Portuguese Russian Chinese and Japanese In addition the Multiskan GO is robotic compatible and can be connected to plate handling devices such as stackers
51. length correction is enabled Thermo Fisher Scientific Kinetic Cuvette results Thermo Fisher Scientific Instrument User Interface Cuvette measurement Use the Kinetic session to perform a kinetic measurement on a cuvette F A Cuvette Kinetic parameters Wavelength nm 25 Pathlength correction Number of readings 100 Interval mm ss 00 00 Total time 0 00 16 The Kinetic submenu contains the following parameters e Wavelength Enter the measurement wavelength The available range is 200 1000 nm e Number of readings Enter the number of readings The range is 1 100 e Interval If the Number of readings is greater than 1 the measurement is considered kinetic and you have to set the time interval 00 00 05 00 When selecting 00 00 the instrument measures as fast as possible The total kinetic measurement time is calculated and displayed e Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength e Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled The results of a cuvette measurement are displayed automatically after the measurement The user cannot save cuvette results to the instrument s memory The last result of all predefined cuvette sessions is automatically saved until parameters are changed or
52. ll plates with PP p and without lids as well as low volume plates preferably a high base type The plate carrier Figure 2 4 acts as the sample holder The maximum plate height of a combined plate and lid is 18 5 mm Cuvette port The Multiskan GO with cuvette Figure 2 4 has an incorporated cuvette port that can measure the absorbance of solutions in suitable cuvettes Cuvette port dimensions for selecting suitable cuvettes are presented in Table 2 1 Table 2 1 Cuvette port properties Cuvette width x depth Cuvette height Measurement beam Beam diameter position 12 5x 12 5 mm 40 58 mm 8 5mm 1 8 mm Beam center height from the bottom of the cuvette port Cuvettes corresponding to the dimensions in the table above can be used with the Multiskan GO cuvette port Refer to Appendix C Example of Available Cuvettes Ensure that the selected cuvette type has a clear window within the measurement window and that the measurement windows of the fully inserted cuvette are aligned with the measurement direction arrow on the cuvette port Figure 2 5 12 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific General Description Optical system Figure 2 5 Aligning the measurement windows of the inserted cuvette Optical system The optical system of the Multiskan GO instrument is shown in Figure 2 6 aay Moving measurement head Mirror optics Detector Microplate reading station 1 Entranc
53. m the zeroing only with an empty instrument O First zero with the empty cuvette port to set the baseline level of the instrument to zero Measure all samples including the blank sample This will also record the blank sample absorbance together with the sample results Subtract manually the blank sample absorbance from all the other samples e The third possible way is to directly zero the instrument with the blank sample cuvette O Zero with the blank cuvette in the port to set the level of the blank sample to zero Note that with this method possible contaminations in the blank sample are not recognized and this can therefore lead to incorrect subtraction of the blank levels Caution Do not perform zeroing with an empty cuvette An empty cuvette has four optical boundary surfaces between air and the cuvette material whereas real sample cuvettes have only two which leads to quite different beam behavior when the measurement beam is transmitted through the cuvette This leads to incorrect zero levels A To zero the cuvette station press the F2 Zero key The yellow triangle alerts the user when the zero is not valid Cuvette Settings Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 41 Instrument User Interface Cuvette measurement When Zero is pressed the instrument measures the current absorbance level and sets it to 0 000 Abs The following applies for zeroing e Itis not possibl
54. med in the Cuvette menu The Cuvette menu contains five predefined measurement sessions Single Spectrum DNA RNA User formula and Kinetic Plate A Settings ket Spectrum DNA RNA User formula Kinetic Results Cuvette sessions or measurement results cannot be stored in the instrument s memory You should export or print measurement results if you wish to use them later The instrument memorizes the most recent measurement parameters and results for each predefined session type The displayed instrument temperature indicates the cuvette incubator temperature A detailed overview of the Cuvette menu is shown in Table 5 6 Thermo Scientific Multiskan GO User Manual 39 Instrument User Interface Cuvette measurement Table 5 6 Cuvette menu parameters Cuvette menu Single L Wavelength 200 1000 nm L Pathlength correction Yes No L Pathlength 0 1 10 0 mm Spectrum L Start wavelength 200 999 nm L End wavelength 201 1000 nm L Step 1 800 nm L Pathlength correction Yes No L Pathlength 0 1 10 0 mm L Mode Fast Precision DNA RNA L Nucleotide RNA dsDNA ssDNA L Pathlength correction Yes No L Pathlength 0 1 10 0 mm User formula L Formula aM1 a A1 A2 a A1 A2 a A1 A3 A2 A3 La 0 0001 1 000 000 L Wavelength 200 1000 nm L Pathlength correction Yes No L Pathlength 0 1 10 0 mm Kinetic L Wavelength 200 1000 nm L Number of readings 1 100 L Interval 0 5 min
55. mo Fisher Scientific representative Error and When an error is detected the current operation is aborted After an W arnin g co d es error it is best to restart from the beginning after the problem has been fixed When an error occurs the error code and the suggested actions are presented Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 67 Troubleshooting Guide Error and warning codes 68 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Chapter 9 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Table 9 12 and Table 9 13 Multiskan GO Table 9 12 Instrument catalog number Code Item 51119200 Multiskan GO w o cuvette 51119250 Multiskan GO w o cuvette Japan 51119300 Multiskan GO with cuvette 51119350 Multiskan GO with cuvette Japan List of spare Table 9 13 Codes for spare parts and accessories parts and Code Item Quantity accesso ri es N10589 Multiskan GO Brief User s Guide English 1 518 119 Skanlt Software for Multiskan GO RE CD 1 N08217 Power supply 1 N09997 Transport lock bar 1 N04001 USB A B device cable 1 8 m 1 longer USB cables available from PC stores Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 69 Ordering Information List of spare parts and accessories 70 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Appendix A System Log Instrument name
56. mperature has been reached Unpack the Multiskan GO instrument and its accessories carefully so that the arrows on the transport packaging point upwards Place the instrument onto a laboratory bench Caution Do not touch or loosen any screws or parts other than those specifically designated in the instructions Doing so may cause misalignment and will void the instrument warranty A Retain the original packaging for future transportation The packaging is designed to ensure safe transport and minimize transit damage Use of insufficient packaging materials may invalidate the warranty Retain all instrument related documentation provided by the manufacturer for future use If you relocate your instrument or ship it for service refer to Packing for service on page 60 Check the enclosed packing list against the order Visually inspect the transport package the instrument and the accessories for any possible damage If any parts are missing or damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy When setting up your Multiskan GO avoid locations with excessive dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations Thermo Scientific Multiskan GO User Manual 15 Installation Installation steps Make sure that The working area is flat dry clean and vibration proof and leave additional room for cables covers etc
57. nd disconnect the power supply cable 4 Disinfect the outside of the instrument or to remove stains using a cloth dampened with 70 ethanol 5 Place the instrument in a large plastic bag Ensure that the cuvette port and the measurement chamber door are open and the plate carrier is Out 6 Place a cloth soaked in the prepared decontaminant solution into the bag Ensure that the cloth does not come into contact with the instrument 7 Close the bag firmly and leave the instrument in the bag for at least 24 hours 8 Remove the instrument from the bag 9 After decontamination clean the instrument using a mild detergent 10 After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 57 Maintenance Refitting the transport lock Refitting the When you relocate the instrument or ship it for service make sure that transp ort lo ck you refit the transport lock Figure 6 16 Figure 6 16 Transport lock bar with warning tag To refit the transport lock 1 Switch the instrument off by pressing and holding the STOP OFF key for a few seconds This drives the measurement head and plate carrier into the correct positions 2 Disconnect the power supply and USB communication cable and any connected peripheral devices 3 Open the me
58. ng Table 2 2 Shaking speeds Speed name Speed Low 5 Hz amplitude 15 mm Medium 11 Hz amplitude 3 mm High 20 Hz amplitude 1 mm USB ports for The USB port can accept an external memory device or printer memory device Sig 23 on page 11 and printer You can transfer any measurement data to the PC with a USB memory device The external printer must support HP PCL 5 PCL5e or PCL5c emulation types Figure 2 3 on page 11 Typically printers with emulation type PCL6 also support PCL5 You can print measurement data with or without printout header texts Skanlt Softwa re The instrument can be controlled with SkanIt Software for Multiskan GO which controls all the instrument functions and provides data processing as well as reporting functions For more information refer to the SkanIt Software for Multiskan GO User Manual Cat no N10597 14 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific What to do upon delivery Unpacking the instrument A Checking delivery for completeness and damage Environmental requirements Thermo Fisher Scientific Chapter 3 installation This chapter describes the installation of the Multiskan GO instrument This section covers the relevant procedures to be carried out on receipt of the instrument Move the packed instrument to its site of operation To prevent condensation the instrument should be left in its protective plastic wrapping until the ambient te
59. ngs in submenus The STOP OFF key e Terminates the measurement process e Terminates the computer remote control Cancels the current setting in submenus Switches the instrument off press and hold Thermo Fisher Scientific Menu structure Session Plate format Area Incubation Measurement Thermo Fisher Scientific Session 96 wells All 00 00 00 Shaking No Required temp No 250 1000 nm 2 nm F Results Instrument User Interface Menu structure The user interface is divided into the Plate Cuvette and Settings menus These main menus are displayed as tabs at the top of the display You can switch between the Plate Cuvette and Settings menus with the LEFT and RIGHT arrow keys when you are on the main menu level The Plate and Cuvette menus are used to define the measurement parameters for microplate and cuvette measurements The Settings menu contains universal instrument configuration settings Cuvette Settings Single amp Configuration rs Spectrum 2 Date amp Time ad DNA RNA Print header LA AJ User formula Status report i Kinetic Results Highlight a menu item using the UP and DOWN arrow keys and select the item by pressing the OK key Selecting an item opens a selection box or enters a submenu in which you can make selections using the arrow keys and the OK key settings Date amp Time parameters Time 14 04 2010 12 27 59
60. nt control and laboratory use EN 61326 2 6 2006 Product family standard Electrical equipment for measurement control and laboratory use in vitro diagnostic IVD medical equipment Test standards Performance limits EN 55011 2007 Class B 150 kHz 1 GHz EN 61000 3 2 2006 Class A EN 61000 3 3 1995 A1 2001 A2 2005 ANSI C63 4 2003 Class B 450 kHz 1 GHz EN 61000 4 2 1995 A1 1998 A2 2001 4kVCD 8kVAD Criteria B EN 61000 4 3 2002 A1 2002 3 V m 80 MHz 2 7 Ghz Criteria A EN 61000 4 4 2004 1 kV Criteria B EN 61000 4 5 2006 2 kV line to ground 1 kV line to line Criteria B EN 61000 4 6 2007 3 Vims 190 kHz 80 MHz Criteria A EN 61000 4 8 1993 A1 2001 3 A m Criteria A EN 61000 4 11 2004 30 500 ms Criteria B 60 100 ms Criteria B 100 10 ms Criteria C 100 20 ms Criteria C 100 5000 ms Criteria C Thermo Fisher Scientific Chapter 8 Troubleshooting Guide Note Do not use the instrument if it appears to malfunction A Handling If there is an abnormal situation during operation such as fluids spilling a bn orm al inside the instrument situations peak Switch off the instrument Figure 2 3 on page 11 2 Unplug the instrument immediately from the power supply Figure 3 11 on page 18 3 Carry out appropriate corrective measures However do not disassemble the instrument 4 If the corrective measures taken do not help contact authorized technical service or your local Ther
61. nt User Interface Cuvette measurement e User formula sessions the result of the user formula calculation is shown with the measured results Cuvette User formula M 13 Tn 454 a 1 0000 M nm A260 0 0501 12 nm A280 0 1680 A3 nm A320 0 2013 e Kinetic sessions the result curve of the kinetic measurement is displayed Cuvette Kinetic Curve 1 50 1 00 HA VW 4 You can export measurement results to a connected USB memory device by pressing the F1 Export key in the result view For more information refer to Export file formats on page 51 To export the cuvette session e Export the current selected cuvette session by pressing the F1 Export key or File gt Export new file in the result view Nucleotide dsDNA 1 35 0 90 A260 A320 gt _ 55 A280 A3205 0 45 M 50 A260 6 83 0 00 i U 1 H 230 250 270 pg ml Thermo Scientific Multiskan GO User Manual 47 Instrument User Interface settings menu CUYOOOUR TAT 10 04 2010 15 59 05 CUYOOOUS TAT 10 04 2010 15 78 36 CUYVOOO04 TAT 10 04 2010 15 76 38 CUYOOOOS TAT 10 04 2010 15 25 45 CUYOOO02 TAT 10 04 2010 15 23 34 CUYOOOO1LTAT 05 04 2010 15 26 46 Printing results Settings menu 48 Thermo Scientific Multiskan GO User Manual e Append the current cuvette session to the end of the selected session on the USB memory device by pressing File gt Export append Press the UP and DOWN arrow keys
62. power failures Intended users How to use this user manual Thermo Scientific Skanit Software Thermo Fisher Scientific About This User Manual The Multiskan GO as standalone or with Thermo Scientific SkanIt Software can be used in research laboratories by professional personnel This user manual is for the following instruments Multiskan GO w o cuvette Cat no 51119200 and 51119250 Japan and Multiskan GO with cuvette 51119300 and 51119350 Japan It aims to give you the information you need for e Reviewing safety precautions e Installing the Multiskan GO e Navigating in the Multiskan GO user interface e Operating the instrument e Programming measurement sessions e Performing basic cleaning and maintenance procedures e Troubleshooting the instrument This user manual also describes all the features and specifications of the Multiskan GO instrument as well as ordering information Note that simulated data appears in the screen captures Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available For SkanIt Software related issues refer to the Skanlt Software for Multiskan GO User Manual Cat no N10597 The SkanIt Software for Multiskan GO Research Edition CD Cat no 5187119 includes the following manuals Multiskan GO User Manual e Multiskan GO Brief Users Gui
63. rument User Interface Plate measurement Plate measurement 32 Thermo Scientific Multiskan GO User Manual Microplate measurements are defined and performed in the Plate menu Cuvette Settings Session12 384 wells A1 H10 1S PEN TA AN 00 02 00 Shaking No Required temp 30 C lt amp Measurement 400 700 nm 10 nm F amp 220c The Plate menu contains settings for the session name plate format measurement area plate incubation and shaking and the measurement type A detailed overview of the Plate menu is presented in Table 5 3 Table 5 3 Plate menu parameters Plate menu Session Session name indicates unsaved results L Select session to open Plate format 96 or 384 wells L 96 wells L 384 wells Area All One well e g A1 Area e g A1 D4 L Selected area All One well e g A1 Area e g A1 D4 Incubation Duration Shaking mode Required temperature L Incubation parameters L Duration hh mm ss L Shaking No Single Double Interval L Speed Low Medium High L Required temp No Temp e g 30 Measurement Measurement information L Measurement parameters L Type Single Multiple Spectrum L Wavelength Wavelength 1 3 Start End wavelength 200 1000 nm L Step 1 800 nm L Mode Fast Precision Thermo Fisher Scientific Session name Thermo Fisher Scientific Plate format Instrument User Interface Plate measurement The Session row in the Plate menu shows
64. ry export them to a connected USB memory device or print them on a connected USB printer To save results in the measurement results view select the F2 Save As key or press the FILE key to open the File menu and select either the Save or Save As menu item e Save Overwrites previous results from the same session e Save As Creates a new session file for the results 1 50 GOS OOSoooscooss SOoooocssoocss 1 00 DOS nm Abs NA T 200 300 400 500 600 700 sio 900 1000 902 1733 nm e 22 0 C Cancel You can export measurement results to a connected USB memory device Figure 5 15 by pressing the F1 Export key in the result view or use the File menu Selecting Export in the Results view automatically exports the current session By using Export in the File menu you can export multiple sessions simultaneously You can also import plate measurement results into SkanIt Software For more information refer to Export file formats on page 51 Thermo Fisher Scientific Printing results Cuvette measurement Thermo Fisher Scientific Instrument User Interface Cuvette measurement Figure 5 15 Multiskan GO with a USB memory device connected You can print measurement results directly to a connected USB printer In addition to session and run information the printed report also includes the print header if it has been defined in the settings Cuvette measurements are configured and perfor
65. s for biohazardous or radioactive waste disposal Warning A used lithium Li battery is regulated waste and must be disposed of according to local regulations The Li battery must only be changed by an authorized service technician Instructions for changing the Li battery are described in the service manual A Thermo Fisher Scientific Thermo Fisher Scientific Maintenance Disposal of the instrument Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country Pollution degree 2 see Safety specifications on page 65 Method of disposal Electronic waste Contaminated waste Infectious waste WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information is available from www thermo com WEEEROHS concerning Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive A Regarding the original packaging and packing materials use the recycling operators familiar to you For more information contact your local Thermo Fisher Scientific representative Thermo Scientific Multiskan
66. s for memory device and printer ooooo 14 San oa na at 14 installato isana TA 15 Whatto doupon delivery acns n E aa 15 Vaiss tall ati ii steps ee ema aa en al na 16 W Qt OS ANd CAUTIONS ba ena E 18 Operational check meter luka orn ee 19 Changing the language masam 20 Serang the Insiriment clock esa as 21 Power Save beat ee en 22 Installing SkanIt Software for Multiskan GO ooooo 22 Routing OSTA ON naa 23 SW ILC NS ON io Voces tat aol anew reat aad emer aa 23 INA CAS URNS plate eren ea ena Pn aa 23 Measure A CUVEE aa be Ia pe Pee 24 STULL GOW em BEE 25 Instrument User Interface o ooooooooo oo oo oo oo oo oo 27 Disparan Ke A E 27 Ment ee oa 29 Instrument Gabah eet eee 30 Plate en en et ai be BN Na pena an 32 Cuvette measurement ooocooooWooooo WooWoW o Wo Wo o Wo o 39 SOLOS Menu ine ee Be erna en tie Daan 48 Thermo Scientific Multiskan GO User Manual 7 Contents Chapter 6 Chapter 7 Chapter 8 Chapter 9 Appendix A Appendix B Appendix C 8 Thermo Scientific Multiskan GO User Manual Expor eOrna ea stipe ea E E AARE 51 MamtenahG Enact 53 Regular and preventive maintenance ooooo 53 Cleaning of the Instrument cyane SA EN 54 Disposal of ia ter Al Sesama nana 56 Decontamination proce dit ees me NE 56 Retitung the transport lockerer as aa aan 58 PACK We TOF se ee N 60 Maintaining a System 100 mem er a e 60 Disposabot the TnS Eden kn knee ban anakan aa an 60 Technica
67. t the transport lock Refer to Refitting the transport lock on page 58 e Close the cuvette port and the measurement chamber door e Use the original packaging for shipping e Pack the instrument according to the packing instructions e Enclose a dated and signed Certificate of Decontamination see Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your local Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department A system log which includes a short summary of the use maintenance procedures error messages and other information about the use of the system can be very useful in properly maintaining the system Refer to Appendix A System Log Copy the table as many times as necessary but leave the blank original inside the user manual If the Multiskan GO is exposed to potentially infectious chemical samples toxic or corrosive chemicals or radioactive chemicals waste management of the complete instrument must be carried out to ensure that there is no risk of contamination Warning Decontaminate the instrument before disposal Refer to cc Decontamination procedure on page 56 A Follow laboratory and country specific procedure
68. temp parameter does not affect the instrument Measurement The Measurement parameters submenu allows you to define the parameters measurement method wavelengths and mode The Multiskan GO provides three measurement methods Single wavelength Multiple wavelength and Spectral scanning measurements Measurement paramete Single cert End wavelength nm Start wavelength n Step nm The Measurement parameters submenu contains the following parameters e Type Select the measurement type Single Multiple or Spectrum e Wavelength Enter the measurement wavelengths In a Single measurement you can define one wavelength In a Multiple measurement you can define up to three measurement wavelengths lt Vone gt is selected by entering 0 zero In a Spectrum measurement you define the start and end wavelengths for a spectral scan The end wavelength must be greater than the start wavelength The start wavelength range is 200 999 nm and the end wavelength range is 201 1000 nm 36 Thermo Scientific Multiskan GO User Manual Thermo Fisher Scientific Instrument User Interface Plate measurement Measurement parameters Measurement parameters Measurement parameters Type Mode Type Multiple Type Spectrum Wavelength nm 450 Wavelength 1 nm 450 Start wavelength nm 200 Wavelength 2 nm 620 End wavelength nm 1000 Wavelength 3 nm None Step nm 2 L 22 0 C Accept Cancel L 22 0 C Ac
69. th affects measurements e Prevent any liquid from entering the instrument e Keep the instrument free of dust and other foreign matter In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance see Cleaning of the instrument on page 54 Clean the keypad surface with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used A Caution Never use acetone as it will damage the covers A Caution Do not autoclave any part of this instrument A Clean the instrument regularly as stated below Caution Although the Multiskan GO is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled water A Caution Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur A 1 Turn the power of
70. the name of the active session Session1l is the default active session until the first Save As operation A session name can be up to 15 characters long You can open a session by pressing the OK key on the Session row or by pressing the FILE key and selecting Open from the FILE menu The list of saved sessions shows the session names and the date and time of the last save You can scroll the session list with the UP and DOWN arrow keys You can store at least one hundred 100 96 well single wavelength measurement sessions in the instrument s memory There are two types of sessions those that have not been run sessions without measurement data and those that have been run sessions containing measurement data Press the F3 Sort key to sort the list of saved sessions by their name or by the date they were last saved You can select the utilized plate format 96 or 384 wells with the Plate format setting Session Session12 Plate format 96 wells 364 wells Area All 00 00 00 Shaking No Enea Required temp No Measurement 450 nm 620 nm F L 22 0 C Accept Cancel The dimensions of the plates when using the instrument user interface are given in Table 5 4 and Table 5 5 Table 5 4 96 well plate dimensions 96 well plate Dimension mm Plate length 85 48 Plate width 127 6 First well position X 14 38 First well location Y 11 24 Corner distance X 99 Corner distance Y 63 Thermo Sci
71. trument User Interface Cuvette measurement The DNA RNA submenu contains the following parameters Nucleotide Select the nucleotide to be measured e RNA Calculate the RNA concentration e dsDNA Calculate the double stranded DNA concentration e ssDNA Calculate the single stranded DNA concentration Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when pathlength correction is enabled User formula Use the User formula session to perform a measurement using a user defined calculation on a cuvette Cuvette User formula parameters Sere ceo Pathlength correction No 1 0000 A1 nm 260 A2 nm 280 A3 nm 320 Cancel The User formula submenu contains the following parameters 44 Thermo Scientific Multiskan GO User Manual Formula Select the predefined formula a Enter the value for constant a The available range is 0 000 1 1 000 000 Wavelength 1 3 Enter the measurement wavelength The available range is 200 1000 nm Pathlength correction Enable or disable pathlength correction Use this setting to normalize measurement results to correspond to a 10 mm pathlength Pathlength Enter the cuvette pathlength The available range is 0 1 10 0 mm Shown only when path
72. ure 3 9 Plate carrier pulled fully out 4 Unfasten the red fixing screw and remove the lock rod from the carrier 5 Push the plate carrier back into the instrument and make sure that the measurement chamber door closes properly Figure 3 10 Figure 3 10 Transport lock removed Thermo Fisher Scientific Thermo Scientific Multiskan GO User Manual 17 Installation Warnings and cautions Connecting the power supply cable A USB ports for printer and memory device Type label Transport lock bar storage position Connecting to a computer Connecting to a printer Warnings and cautions 18 Thermo Scientific Multiskan GO User Manual Store the transport lock bar on the back of the instrument Figure 3 11 for use in future instrument relocation or transportation To connect the power supply cable Warning Never operate your instrument from a power outlet that has no ground connection Never use a power supply cable other than the Thermo Scientific power supply cable designed for your region A 1 Connect the power supply to a correctly installed line power outlet that has a protective conductor that is grounded 2 Connect the power supply cable to the power input socket Figure 3 11 at the back of the instrument Power supply connector Air inlet USB port for PC connection Figure 3 11 Connecting the power supply cable If you are using a computer with the Multiskan GO connect the communic

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