JA-10 Fixed Angle Rotor
Contents
1. 420 267 00 83 23 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 365 3707 Fax 41 22 365 0700 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 33 1 49 90 90 00 Fax 33 1 49 90 90 10 e mail bioresearch france beckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 51 33 35 Fax 49 21 51 33 33 e mail bioresearch de beckman com GS zraou COULTER Beckman Coulter Inc e Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 Fax 852 2873 4511 INDIA Beckman Coulter India Pvt Ltd Solitaire Corporate Park 3rd Floor Bldg 11 Andheri Ghatkopar Link Road Chakala Andheri West Mumbai India 400 098 Telephone 91 22 3080 5101 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 39 02 953921 Fax 39 02 95392264 JAPAN Beckman Coulter K K TOC Ariake West Tower 2 5 7 Ariake Koto ku Tokyo 135 0063 Japan Telephone 81 3 5530 8500 Fax 81 3 5404 8436 MEXICO Beckman Coulter de Mexico S A de C V Avenida Po2. Angle Rotor Table 1 Available Tubes and Bottles for the JA 10 Fixed Angle Rotor Use only the items listed here and observe fill volumes and maximum run speeds Tube Required Accessory Max No Max Tubes Max Dimensions Part Fill Vola Part per Speed and Volume Description Number mL Description Number Adapter RCF 69 x 160 mm polycarbonate 355605 465 none 10 000 rpm 500 mL bottle w cap assy pkg 6 17 700 xg 69 x 160 mm polypropylene 355607 445 none 8 000 rpm 500 mL bottle w cap assy pkg 6 11 300 x g 62 x 120 mm polypropylene 356011 250 adapter 362750 1 10 000 rpm 250 mL wide mouth pkg 6 PET pkg 2 17 700xg bottle w cap assy 62 x 120 mm polycarbonate 356013 250 adapter 362750 1 10 000 rpm 250 mL wide mouth pkg 6 PET pkg 2 17 700xg bottle w cap assy 29 x 104mm polycarbonate 357000 45 adapter 356996 1 10 000 rpm 50 mL bottle assy pkg 6 polypropylene pkg 1 12 000 xg w liquid tight cap assy 29 x 104mm polyallomer 357001 45 adapter 356996 1 10 000 rpm 50 mL bottle assy pkg 6 polypropylene pkg 1 12 000 x g w liquid tight cap assy 29 x 104mm polycarbonate 357002 40 adapter 356996 1 10 000 rpm 50 mL bottle w screw pkg 25 polypropylene pkg 1 12 000 x g cap 29 x 104mm polyallomer 357003 40 adapter 356996 1 10 000 rpm 50 mL bottle w screw pkg 25 polypropylene pkg 1 12 000 x g cap 29 x 104mm polypropylene 357005 36 5 adapter 356996 1 10 00
3. Beckman Coulter J2 J6 and Avanti J series centrifuges the rotor develops centrifugal forces that can efficiently pellet bacterial cells from large volumes or particles from tissue homogenates Up to 3 liters of sample and gradient can be centrifuged per run The rotor body and lid are made of aluminum and are anodized for corrosion resistance the rotor is black and the lid is blue The lid knob secures the rotor to the drive hub O rings made of Buna N rubber in the lid and rotor body maintain atmospheric pressure inside the rotor during centrifugation if they are properly lubricated Pins in the rotor drive hole mate with grooves in the centrifuge drive hub older model centrifuges or with drive hub teeth new model centrifuges to prevent the rotor from slipping during acceleration and deceleration Starting in 2002 JA 10 rotors are manufactured with magnets embedded in the rotor base for rotor identification in the Avanti J E centrifuge Older JA 10 rotors without magnets can be used in the Avanti J E but the rotor speed will be limited to 6300 rpm Other Avanti J series and J2 series centrifuges use alternate methods of rotor identification that enable the rotor to run up to 10 000 rpm The JA 10 rotor is warranted for 7 years see the Warranty PREPARATION AND USE Specific information about the JA 10 rotor is given here Use the J Series Rotors and Tubes manual JR IM along with this rotor manual for complete rotor
4. Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor charac teristics or is operated in a Beckman Coulter centrifuge that has been improperly disassembled repaired or modified DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 1 905 819 1234 Fax 1 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 CZECH REPUBLIC Beckman Coulter Prague Radiova 1 102 27 Prague 10 Czech Republice Telephone 420 267 00 85 13 Fax
5. display and the display flashes SET COMP JA 10 Fixed Angle Rotor 4 Use the keypad to enter the desired compensation value Press the key to enter a minus sign pressing it again will remove the minus sign 5 Check the temperature display If the entry is incorrect press CE and reenter the digits 6 When the entry is correct press ENTER RECALL IlIi NOTE To clear a COMP ADJ entry press COMP ADJ 0 and ENTER RECALL Analog J2 Series Centrifuges Enter the required run temperature and the appropriate temperature compensation units see Table 2 on the centrifuge control panel as follows 1 Turn the SET knob to the required sample temperature 2 Find the compensation value that corresponds to the required temperature and run speed The compensation values listed in Table 2 are approximate for all centrifuge models If precise temperature control is required follow the steps in Microprocessor Controlled J2 and J6 Series Centrifuges above Table 2 Temperature Compensation Settings for J2 and J6 Series Centrifuges Interpolate if intermediate values are required Rotor Required Sample Temperature C green bar Speed rpm 20 10 po 5S 10 20 40 10 000 N 3 2 1 0 1 2 8 000 3 2 1t 1t 0 1 2 5 000 2 0 0 0 0 1 2 N indicates that the rotor cannot achieve the required temperature at this speed t For the J2 HC cent
6. 0 rpm 50 mL thickwall tube pkg 25 polypropylene pkg 1 12 000 xg w snap on ca R natural 29 mm cap 3562644 red 29 mm cap 3573594 green 29 mm cap 3573604 yellow 29 mm cap 3573614 blue 29 mm cap 3573624 a Above 20 C fill polypropylene tubes at least half full Continued b Maximum speeds listed are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of manufacturing variances no guarantee of performance or fit is expressed or implied Polyethylene teraphthalate d Polypropylene package of 25 e Delrin is a registered trademark of E I Du Pont de Nemours amp Company f Commercially available Observe manufacturer s speed and fill volume guidelines JA 10 Fixed Angle Rotor Table 1 Available Tubes and Bottles for the JA 10 Fixed Angle Rotor continued Tube Required Accessory Max No Max Tubes Max Dimensions Part Fill Vola Part per Speed and Volume Description Number mL Description Number Adapter RCF 29 x 104mm polypropylene 357007 50 adapter 356996 1 10 000 rpm 50 mL thickwall tube pkg 25 polypropylene pkg 1 12 000 xg no cap 29 x 104mm polycarbonate 363647 40 adapter 356996 1 10 000 rpm 50 mL open top tube pkg 25 polypropylene pkg 1 12 000 x g 29 x 104mm polycarbonate 363664 40 adapter 356996 1 10 000 rom 50 mL tube w snap pkg 25 polypropylene pkg 1 12 000 xg on cap 2
7. 9x 115mm conical tube f f adapter 356965 1 10 000 rpm 50 mL polypropylene pkg 1 12 000 xg 17x 120mm conical tube f f adapter 356960 5 6 500 rpm 15 mL polypropylene pkg 1 7 000 x g 17x 120mm round bottom f f adapter 356994 5 10 000 rpm 15 mL tube polypropylene pkg 1 12 000 x g 13 x 100 mm round bottom f f adapter 356973 10 000 7 mL tube Delrine pkg 1 12 000 x g 12 x 75mm round bottom f f adapter 356967 10 000 4mL tube Delrine pkg 1 12 000 xg Bio Vials polypropylene 566353 4 adapter 342098 9 10 000 rpm 14x55mm_ capped pkg 1000 Delrine pkg 1 4mL adapter PET 362750 1 pkg 2 a Above 20 C fill polypropylene tubes at least half full b Maximum speeds listed are guidelines only These speeds have been achieved in reliability tests at Beckman Coulter but because of manufacturing variances no guarantee of performance or fit is expressed or implied Polyethylene teraphthalate 4 Polypropylene package of 25 e Delrin is a registered trademark of E I Du Pont de Nemours amp Company f Commercially available Observe manufacturer s speed and fill volume guidelines m Polycarbonate and Polyallomer Bottles Capped polycarbonate and polyallomer bottles may be run completely filled or partially filled not less than half full All opposing containers for a run must be filled to the same level JA 10 Fixed Angle Rotor OPERATION e Precool the rotor in the centrif
8. COULTER 2007 Beckman Coulter Inc Printed in the U S A J TB 006Z December 2007 JA 10 Fixed Angle Rotor Used In Beckman Coulter J2 J6 and Avanti J Series Centrifuges JA 10 Fixed Angle Rotor i ie ies le Te SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equip ment is attempted When you see the symbol on other pages throughout this publication pay special attention to the specific safety information presented Observance of safety precau tions will also help to avoid actions that could damage or adversely affect the performance of the rotor This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centri fuge that has been modified without Beckman Coulter s approval Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol pr
9. Cleaning Kit 339558 contains two special plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water gt NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long periods such as overnight 3 Thoroughly rinse the cleaned rotor and components with distilled water 4 Air dry the rotor and lid upside down Do not use acetone to dry the rotor 5 Apply a thin even coat of silicone vacuum grease to the lid O ring before replacing it in the groove in the outer rim of the lid Also apply silicone vacuum grease to the rotor O ring before reassembly Clean metal threads every 6 months or as necessary Use a brush and concentrated Solution 555 Rinse and dry thoroughly then lubricate lightly but evenly with Spinkote to coat all threads Periodically remove the O rings and wipe clean as necessary Clean the O ring grooves with a cotton tipped swab Reapply a light film of silicone vacuum grease If the rotor and or accessories becomes contaminated with radioac tive material it should be decontaminated using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash gt 4 In U S contact Nuclear Associates New York in Eastern Europe and Commonwealth Stat
10. Figure 4 Knowing the pin orien tation before you install the rotor will help to ensure that you position the rotor properly on the hub minimizing the chance of hub damage Follow the steps below 1 Turn the rotor until the BECKMAN name is either horizontal or perpendicular to the drive hub pins or teeth see Figure 4 Alter nately rotate the drive spindle to align the hub pins or teeth with the rotor drive pins 2 Delrin is a registered trademark of E I Du Pont de Nemours amp Company JA 10 Fixed Angle Rotor Rotor Drive Hole Drive Pins maybe horizontal vertical or angled Avanti J Centrifuge Drive Spindle Hub Figure 2 Rotor Drive Pin Location and Orientation 2 Carefully lower the rotor straight down onto the centrifuge drive spindle hub Do not drop the rotor onto the hub 3 Slowly turn the rotor making sure that it is properly seated on the drive spindle hub In rare cases the rotor pins can rest on top of the hub pins or teeth and not seat on the hub surface This can prevent the rotor from being secured to the drive spindle Turning the rotor slightly after installing it will move the pins into proper position Drive Rotor Hub Pins 2 Teeth 2 Grooves 4 Drive Hub Drive Hub Pe Drive as Drive Spindle Spindle Assembly Assembly Older Model Centrifuges Newer Model Centrifuges Be sure the pins in the rotor drive hole Be sure the pins in the rotor drive hole are seated in the groov
11. and accessory operation Newer JA 10 rotors with magnets p n 369687 cannot be used in J2 or J6 series centrifuges Older JA 10 rotors without magnets p n 334833 can be used in J2 and J6 series centrifuges PRERUN SAFETY CHECKS A ROTOR PREPARATION JA 10 Fixed Angle Rotor IIIi NOTE Although rotor components and accessories made by other manufacturers may fit in the JA 10 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in this rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Read the Safety Notice page at the front of this manual before using the rotor 1 Make sure that the rotor and lid are clean and show no signs of corrosion or cracking Check the chemical compatibilities of all materials used refer to Appendix A in Rotors and Tubes Verify that the tubes and bottles being used are listed in Table 1 For runs at other than room temperature refrigerate or warm the rotor beforehand for fast temperature equilibration 1 Apply a thin film of silicone vacuum grease 335148 to the O rings 2 Load the filled containers symmetrically into the rotor Refer to TUBES AND BOTTLES on page 6 for information about containers If fewer than six tubes are being run they must be arra
12. e be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all cen trifuge parts required for repair REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter Inc be returned to the factory transporta tion charges prepaid and will be returned to Buyer with the transportation charges collect unless the product is found to be defective in which case Beckman Coulter Inc will pay all transportation charges A defective rotor will be replaced by Beckman Coulter Inc at its then current list price less a credit based upon the age of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Palo Alto California facility or delivered to a Beckman Field Service representative The replacement price cost to Buyer for the respective rotor shall be calculated as follows Replacement price Current rotor list price x years 7 CONDITIONS 1 Except as otherwise specifically provided herein this war ranty covers the rotor only and Beckman Coulter Inc shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance con trary to the instructions in the
13. eavy discoloration If any of these signs are evident do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center e Regularly lubricate the metal threads in the rotor with a thin even coat of Spinkote lubricant 306812 Failure to keep these threads lubricated can result in damaged threads e Regularly apply silicone vacuum grease 335148 to the lid O ring 870139 and rotor O ring 870980 Replace O rings about twice a year or whenever worn or damaged e Routinely apply Spinkote lubricant 306812 to the centrifuge drive spindle to prevent the rotor from sticking not required on Avanti J series centrifuges Refer to Appendix A in Rotors and Tubes for chemical compatibili ties of rotor and accessory materials Your Beckman Coulter repre sentative provides contact with the Field Rotor Inspection Program and the rotor repair center Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues 17 JA 10 Fixed Angle Rotor DECONTAMINATION 1 Remove the O rings before washing 2 Wash the rotor and lid in a mild detergent such as Beckman Solution 555 339555 that won t damage the rotor The Rotor
14. es contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 5 In U S contact Biodex Medical Systems Shirley New York internationally contact the U S office to find the dealer closest to you 18 JA 10 Fixed Angle Rotor I gt NOTE IsoClean can cause fading of colored anodized surfaces Use it only when necessary and remove it promptly from surfaces While Beckman Coulter has tested these methods and found that they do not damage components no guarantee of decontamination is expressed or implied Consult your laboratory safety officer regarding the proper decontamination methods to use If the rotor or other components are contaminated with toxic or patho genic materials follow appropriate decontamination procedures as outlined by your laboratory safety officer STERILIZATION AND DISINFECTION 121 C Guu mA STORAGE e The rotor and all rotor components can be autoclaved at 121 C for up to an hour Remove the lid from the rotor and place the rotor and lid in the autoclave upside down Ethanol 70 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage the ro
15. es are not sitting on top of the teeth of the drive spindle hub as shown on the drive spindle hub Figure 3 Centrifuge Drive Spindle Hub Configurations 10 JA 10 Fixed Angle Rotor Top View Side View Figure 4 Installing the Rotor 4 To secure the rotor to the drive spindle place the lid on the rotor and turn the lid knob to the right clockwise until secure Do not overtighten REMOVAL AND SAMPLE RECOVERY AN CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 Unscrew the rotor lid to release the rotor from the spindle hub 2 Remove the rotor by lifting it straight up and off the drive spindle If the rotor sticks to the drive spindle hub use the rotor removal tool 334827 to release the rotor as follows a Screw the threaded end of the rotor removal tool into the rotor drive hole b When the removal tool contacts the drive spindle hub continue turning the removal tool until the rotor releases c Unscrew and remove the removal tool from the rotor The rotor should now lift easily off of the drive spindle hub 11 JA 10 Fixed Angle Rotor 12 TEMPERATURE TEMP C Lf To ensure that the JA 10 rotor reaches the required temperature during the run follow the appropriate instructions below for the model of J centrifu
16. ge being used Avanti J Series Centrifuges Enter the run temperature according to the instructions in your centri fuge instruction manual No additional input is required Microprocessor Controlled J2 and J6 Series Centrifuges Models J2 MI J6 MI J2 MC and J6 MC Enter rotor code 10 then follow the steps below 1 Press the TEMP key on the centrifuge control panel and then use the keypad to enter the sample temperature 2 Find the compensation value in Table 2 that corresponds with the set temperature and run speed The compensation values listed in Table 2 are approximates for all centrifuge models If precise temperature control is required determine the compensation settings empirically as follows a Fill the rotor with water filled tubes or bottles Precool the rotor and water to the required temperature Place the filled precooled rotor in the centrifuge and run the system for at least 2 hour Measure the water temperature If it is lower than the required run temperature enter positive compensation units during run setup following steps 3 and 4 below If it is higher than the required run temperature enter negative compensation units during run setup also using steps 3 and 4 below Discard the water Fill tubes with sample load tubes into the rotor and precool the system to the required temperature before starting the run 3 Press COMP ADJ The word COMP flashes below the TEMPERATURE
17. nged symmetrically in the rotor see Figure 1 Opposing tubes must be filled to the same level with liquid of the same density within 10 grams JA 10 Fixed Angle Rotor TUBES AND BOTTLES 25 C XN 0 C NS O O oO SES Figure 1 Arranging Tubes or Bottles in the Rotor Two three four or six tubes or bottles can be run if they are arranged symmetrically in the rotor as shown The JA 10 rotor uses the tubes and bottles listed in Table 1 Use only those items listed and observe the maximum speed limits and fill volumes shown Maximum fill volume is the maximum amount that can be centrifuged in the container listed Refer to Appendix A in Rotors and Tubes for chemical compatibilities of tube bottle and accessory materials Temperature Limits e Plastic containers have been centrifuge tested for use at temp eratures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated run conditions e If plastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation Thickwall Tubes Thickwall polyallomer polypropylene and polycarbonate tubes can be run partially filled at least half filled with or without caps but all opposing tubes for a run must be filled to the same level with liquid of the same density Do not overfill capless tubes be sure to note the fill volumes and run speeds shown in Table 1 JA 10 Fixed
18. ntrifugal Fields for the JA 10 Rotor Entries in this table are calculated from the formula RCF 1 12 r RPM 1000 and then rounded to three significant digits Relative Centrifugal Field x g Rotor Speed At Tmax At Tay At min rpm 158 mm 98 mm 38 mm 10 000 17 700 11 000 4 260 9 500 16 000 9910 3 840 9 000 14 300 8 890 3 450 8 500 12 800 7 930 3 070 8 000 11 300 7 020 2 720 7 500 9 950 6 170 2 390 7 000 8 670 5 380 2 090 6 500 7 480 4 640 1 800 6 000 6 370 3 950 1 530 5 500 5 350 3 320 1 290 5 000 4 420 2 740 1 060 4500 3 580 2 220 861 4 000 2 830 1 760 680 3 500 2170 1 340 521 3 000 1 590 987 383 2 500 1110 686 266 2 000 707 439 170 1 500 398 246 95 1 000 176 109 42 Relative Centrifugal Fields JA 10 Rotor 18 000 16 000 14 000 12 000 10 000 8 000 RCF x g 6 000 4000 2 000 0 2 000 4 000 6 000 8 000 10 000 Speed rpm 16 JA 10 Fixed Angle Rotor CARE AND MAINTENANCE MAINTENANCE Check for Corrosion CLEANING Rotor Fa Body Illi NOTE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use e Periodically at least monthly inspect the rotor especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or h
19. otection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the centrifuge Although rotor components and accessories made by other manufacturers may fit in the JA 10 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the JA 10 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Do not run an empty rotor Place filled tubes in at least two opposing cavities Make sure that filled containers are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with liquid of the same density If disassembly reveals evidence of leakage y
20. ou should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use JA 10 Fixed Angle Rotor JA 10 FIXED ANGLE ROTOR Axis of Rotation SPECIFICATIONS Maximum speed 0 0 0 0 cece eee eee 10 000 rpm Critical speed range 2 0 0 0 cee eee tipari 600 to 800 rpm Maximum solution density 0 000000020 1 2 g mL Maximum allowable imbalance of opposing loads 10 grams Relative Centrifugal Field at maximum speed Attar LOS MNI oati ao i ana Ieee etd ER 17 700 x g Atra OS MM SE 11 000 x g Atran OS mMM srai ad ng iE aia E S 4 260 x g k factor at maximum speed 0 0 00 e eee eee eee 3610 Number of bottle cavities 00 2 eee ee eee 6 Available bottles and tubes 0 000 see Table 1 Nominal dimensions of largest bottle 69 x 160 mm Nominal bottle capacity 0 0 0 eee eee eee ee 500mL Nominal rotor capacity 0 2 ee eee eee 3 liters Approximate acceleration time to maximum speed rotor fully loaded 0 0 0 eee ee eee 4 1 2 min Appro
21. pocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 001 52 55 9183 2800 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 31 297 230630 Fax 31 297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A Primegro Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za SPAIN Beckman Coulter Espafia S A C Caleruega 81 28033 Madrid Spain Telephone 34 91 3836080 Fax 34 91 3836096 email bioresearch espana beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 111 56 SE 168 11 Bromma Sweden Telephone 46 8 564 85 900 Telefax 46 8 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 0800 850 810 Fax 41 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kar
22. ree from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office JA 10 Fixed Angle Rotor SUPPLY LIST I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Contact Beckman Coulter Sales 1 800 742 2345 in the United States or your local Beckman Coulter office or see the Beckman Coulter High Performance High Speed High Capacity Rotors Tubes and Accessories catalog BR 8102 available at www beckmancoulter com for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS JA 10 rotor assembly
23. rifuge use a setting of 0 instead of 1 13 JA 10 Fixed Angle Rotor 14 RUN TIMES TIME HR MIN 12 Z j7 U I a 9 l 3 Based on sedimentation in water at 20 C The k factor of the rotor is a measure of the rotor s pelleting effi ciency Beckman Coulter has calculated the k factors for all of its rotors at maximum speed with full tubes using the following formula In Fmax nin 7 10 1 o2 3600 where wis the angular velocity of the rotor in radians per second 0 105 x rpm Fmax is the maximum radius and 7 is the minimum radius After substitution 11 p n 2 533 x 10 In inax min s rpm In the JA 10 rotor the k factor is 3610 for full 500 mL bottles at maximum speed For containers that nominally hold less than 500 mL the k factors will be different Calculate the k factors for these containers using equation 2 Use the k factor in the following equation to estimate the run time t in hours required to pellet particles of known sedimentation coeffi cient s in Svedberg units S 3 S For example at 10 000 rpm E coli s 3600 S will pellet in 3610 t 3600 1 hour 4 Run times can be estimated for centrifugation at less than maximum speed by adjusting the k factor as follows 2 10 000 ba Fema LD adj Kratea ctual run speed JA 10 Fixed Angle Rotor Run times can also be estimated from data established in prior experi men
24. tal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 44 01494 441181 Fax 44 01494 447558 e mail beckman_uk beckman com Additional addresses are available at www beckmancoulter com 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 Internet www beckmancoulter com Telex 678413 Fax 1 800 643 4366 2007 Beckman Coulter Inc G Bd Printed on recycled paper
25. tor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included in each box of tubes or bottles for tube and bottle sterilization and disinfection procedures When the rotor is not in use store it in a dry environment not in the instrument with the lid removed to allow air circulation so moisture will not collect in the tube cavities 6 Flammability hazard Do not use in or near operating centrifuges 19 JA 10 Fixed Angle Rotor 20 RETURNING A ROTOR Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form may be obtained from your local Beckman Coulter sales office It should contain the following information e serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and e name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are f
26. ts using a different rotor if the k factor of the previous rotor is known For any two rotors a and b t ks ee th ky where the k factors have been adjusted for the actual run speed used For more information on k factors see Use of k Factor for Estimating Run Times from Previously Established Run Conditions publication DS 719 RUN SPEEDS The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields RCF When rota Eag DETI tional speed is adjusted so that identical samples are subjected to the same rcf in two different rotors the samples are subjected to the same force The RCF at a number of rotor speeds is provided in Table 3 SPEED RPM RCF Do not select rotational speeds higher than the maximums you have determined to be appropriate for your protocols in no case above 10 000 rpm or the limit shown in Table 1 In addition when centrifuging solutions more dense than 1 2 g mL in this rotor calcu late the reduced maximum allowable rotor speed 1 2 g mL density of tube contents reduced maximum speed 10 000 rpm 7 Note however that the use of this formula may still produce maximum speeds higher than the limitations imposed by the use of certain tubes or adapters see Table 1 In such cases use the lower of the two figures 15 JA 10 Fixed Angle Rotor Table 3 Relative Ce
27. uge or in a refrigerator before use ROTOR ID especially before short runs to ensure that the rotor reaches the set temperature A suggested precooling cycle is a minimum of pad ig 30 minutes at 2000 rpm at the required temperature e If fluid containment is required use capped tubes or bottles It is strongly recommended that all containers carrying physiological fluids be capped to prevent leakage e J2 and J6 series centrifuges only Apply a thin film of Spinkote lubricant 306812 to the centrifuge drive hub and to the metal threads on the lid knob e Ifyou are using an Avanti J series centrifuge except Avanti J E select the JA 10 rotor e Ifyou are using an Avanti J E or a microprocessor controlled J2 or J6 series centrifuge enter rotor code 10 INSTALLING THE ROTOR Lubricate J2 and J6 series centrifuge drive hubs with a thin even coat of Spinkote 396812 to prevent the rotor from sticking Avanti J series centrifuge hubs have Delrin rings to prevent rotor sticking and do not require lubrication Two metal pins inside the rotor drive hole engage with teeth on the centrifuge drive spindle hub to prevent the rotor from slipping during acceleration and deceleration see Figure 2 When the rotor is properly installed the pins sit either in the drive hub grooves or on top of the hub surface see Figure 3 The rotor pins are positioned parallel to the BECKMAN name engraved at the center of the rotor body see
28. with magnets rotors manufactured Jan 2002 and later cannot be used in J2 J6 series 369687 JA 10 rotor assembly rotors manufactured through Dec 2001 334833 Lid assembly onr ea 08 a ails gba teas Bohs bobo nis Seales 392122 Lid O ring large 2 cee eee 870139 Rotor O ring small 0 0 eee ee eee 811656 Tiedown kitre e satus ice a ita ee Stats BE ee ees 364911 OTHER Tubes bottles and accessories 0 0 0 0 eee eee see Table 1 Spinkote lubricant 2 oZ n susuan nanea ee eee 306812 Silicone vacuum grease 1 OZ 2 2 eee eee ee eee 335148 Rotor Cleaning Kit 0 0 0 0 eee ee ee eee 339558 Beckman Solution 555 1 qt 2 2 2 ee eee eee eee 339555 Rotor cleaning brush 2 0 00 0 ii ee eee 339379 Rotor removal tool 0 0 0 cc eee eee 334827 21 J SERIES ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions of sale in effect at the time of sale Beckman Coulter Inc agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a J series rotor to the original buyer by Beckman Coulter Inc or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter Inc discloses that such defect developed under normal and proper use Should a Beckman Coulter cen trifug
29. ximate deceleration time from maximum speed rotor fully loaded max brake 0 000 000 eee eee 5 min Weight of fully loaded rotor 00000 22 kg 48 1b Rotor material 0 2 eee ee aluminum Conditions requiring speed reduction see RUN SPEEDS Rotor entry code for Avanti J E and microprocessor controlled J2 and J6 centrifuges 0 0000 10 The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 1 amp where r is the radius in millimeters is the angular velocity in radians per second 2 z RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution RPM 2 RCF 1 12r FM JA 10 Fixed Angle Rotor DESCRIPTION Lid 334492 Lid O ring Pa 870139 mA Rotor O ring 870980 This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges The JA 10 fixed angle rotor rated for 10 000 rpm is designed to centrifuge up to six 500 mL containers at a 25 degree angle to the axis of rotation Used in
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