SX4750 Swinging Bucket Rotor
Contents
1. 0 e eee ee eee eee 4 Available tubes and bottles 0 0 2 0 0000 see Table 2 Maximum load allowed in each tube and bottle bucket at rated speed excluding weight of bucket and cover 1000 grams Maximum load allowed in each multiwell plate carrier at rated speed excluding weight of carrier and cover 360 grams Total maximum allowable imbalance of opposing loads 6 grams Maximum rotor capacity 2 ee eee 3 liters Approximate acceleration time 0 000 0000 80 seconds Approximate deceleration time 000 80 seconds Weight of fully loaded rotor buckets with covers 13 3 kg 29 3 Ib Rotor yoke material 0 00 0 00 e eee eee eee stainless steel Bucket and carrier material anodized aluminum The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through the critical speed range is characterized by some vibration A short duration second critical may occur at about 1300 rpm Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 1 amp where r is the radius in millimeters is the angular velocity in radians per second 2 RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution2. 0 00000000000 00000000000 00000000000 00000000000 00000000000 00000000000 00000000000 OOO0O000000000 OOO0OO000000000 O0Q000000 O0000000 OOO0Q0Q0000000 OO0O0000000000 Examples of Symmetrically Loaded Multiwell Plates load opposite plates the same way INCORRECT Q00000000000 ccaceceuocee CO 00 000000 OO00000000000 QOOOOO000000 000000000000 Examples of Nonsymmetrically Loaded Multiwell Plates Figure 2 Examples of Correctly and Incorrectly Loaded Buckets and Carriers Contents of opposing buckets must be the same and each bucket must be balanced on its pivotal axis 11 SX4750 Swinging Bucket Rotor USING TUBE AND BOTTLE BUCKETS 12 Whether you are running tubes bottles cell culture flasks or blood bags you must load the buckets symmetrically around the center of rotation and each bucket must be loaded symmetrically with respect to its pivotal axis See SYMMETRICAL AND BALANCED LOADING above A CAUTION Buckets are designed to be used only with modular disk adapters bottle adapters cell culture flask adapters Aerosolve cannisters and blood bag cups Do not pour samples directly into buckets bottle sleeves or blood bag cups Do not load bottles cell culture flasks or tubes directly into the buckets Do not use labware that is not specified for use in this rotor You can
3. reduced maximum speed 4450 rpm ee heaviest load in grams Do not select rotational speeds in excess of 4750 rpm for tube and bottle buckets or 4450 rpm for multiwell plate carriers in an Allegra X 15R or 3750 rpm for either buckets or carriers in an Allegra X 12 series centrifuge Up to three Beckman Coulter plates can be run per carrier at maximum multiwell plate speeds If running four Beckman Coulter plates reduce run speed to 2700 rpm Observe manufacturer s run speed and stacking limitations if using other manufacturers multiwell plates If using multiwell plates for runs exceeding 2 hours in a warm and or humid environment speed reduction may be required to maintain low sample temperatures SX4750 Swinging Bucket Rotor CARE AND MAINTENANCE MAINTENANCE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use e Periodically at least monthly inspect the rotor yoke buckets and or multiwell plate carriers especially inside cavities for rough spots or pitting white powder deposits frequently aluminum oxide or heavy discoloration If any of these signs are evident do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center e Approximately every 400 runs and after cleaning and or auto claving wipe the
4. GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 51 33 35 Fax 49 21 51 33 33 e mail bioresearch de beckman com zraou COULTER Beckman Coulter Inc Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 Fax 852 2873 4511 INDIA Beckman Coulter India Pvt Ltd Solitaire Corporate Park 3rd Floor Bldg 11 Andheri Ghatkopar Link Road Chakala Andheri West Mumbai India 400 098 Telephone 91 22 3080 5101 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 39 02 953921 Fax 39 02 95392264 JAPAN Beckman Coulter K K TOC Ariake West Tower 2 5 7 Ariake Koto ku Tokyo 135 0063 Japan Telephone 81 3 5530 8500 Fax 81 3 5404 8436 MEXICO Beckman Coulter de Mexico S A de C V Avenida Popocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 001 52 55 9183 2800 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 31 297 230630 Fax 31 297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336
5. Grasp the carriage by the handles and lower it into the carrier 3 Make sure that carrier cover gaskets are in good condition and secured to the covers 4 Put covers on the carriers with the latches perpendicular to the pin sockets Snap the latches down and secure them under the catrier rim 5 Attach each carrier to the yoke by aligning the grooves in the sides with the pivot pins then sliding the carrier down until the pivot pins are seated in the pin pockets 5 See Table 4 on page 28 for plates recommended for use in stacks of four at maximum speed 18 SX4750 Swinging Bucket Rotor A CAUTION All four positions must contain buckets or carriers during a run Never run the rotor with only two positions filled If two tube and bottle buckets and two multiwell plate carriers are run position the similar buckets opposite each other Select multiwell plate carrier speed when running mixed sets 6 Gently swing the carriers to ensure that they are properly seated on the pivot pins OPERATION 1 Refer to the instrument instruction manual for centrifuge operation 2 See RUN SPEEDS page 20 for information about speed limitations REMOVAL AND SAMPLE RECOVERY AN CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories 1 Remove the buckets or carriers from the centrifuge 2 Remove the
6. RPM 2 RCF 1 12r F RCF inside buckets is 5095 x g tube and bottle buckets r max 201 6 mm or 3919 x g multiwell plate carriers r 176 7 mm SX4750 Swinging Bucket Rotor DESCRIPTION Carrier This rotor has been manufactured in an ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges The SX4750 rated for 4750 rpm 3750 rpm in an Allegra X 12 series centrifuge is a four place swinging bucket rotor used in Beckman Coulter Allegra X 12 series and Allegra X 15R benchtop centrifuges The rotor buckets carry adapters that allow centrifugation of a wide range of tube and bottle sizes from 1 5 mL reaction vials to 750 mL bottles or single to quad pack blood bags as well as multiwell plates in carriers Special adapters are also available that allow centrifugation of Corning 75 cm and 25 cm cell culture flasks The rotor develops centrifugal forces that are suitable for rapidly sedimenting protein precipitates large particles cells and cell debris Figure 1 shows the rotor and accessories The rotor yoke is made of stainless steel Black anodized aluminum buckets and carriers can be run by placing them over pivot pins on the arms of the yoke they swing out to horizontal position during centrifugation Transparent covers made of a high impact plastic are available for the rotor buckets and carriers Covers have been tested to demonstrate containment of microbiologi
7. SNAP ON CAPS continued 11 x 38mm 1 5 orange 356094 359469 359148 polypropylene 354511 354495 11 x 38 mm 1 5 polypropylene 343169 359469 359148 cap separate 354511 354495 11 x 39mm 1 8 white 340196 359469 359148 polyethylene 354511 354495 29 x 103 mm 50 polypropylene 357005 359474 359153 359486 359164 29 x 103 mm 50 polycarbonate 363664 359474 359153 359486 359164 CONICAL TUBES 62 x 141 mm 230 polycarbonate 356987 349946 356983 with cap 356985 62 x 141 mm 230 polypropylene 356989 349946 356983 with cap 356985 BIOVIALS 14x 55 mm 4 polypropylene 566353 359470 359149 344517 each BOTTLES 29 x 104 mm 50 polycarbonate 361693 359474 359153 with cap assembly 359486 gt 3591646 29 x 104 mm 50 polycarbonate 357002 359474 359153 with screw cap 359486 gt 359164 a Fill tubes at least half full Speed reduction may be required for fill volumes below 75 percent Continued b Adapter used in Aerosolve cannister part number 359232 Tube retainers and adapter plates are sold individually d These tubes cannot be run with bucket covers in place e Do not load the 750 mL bottle directly into the bucket always use the adapter f Replacement bottle cap part number is 356263 set of 6 25 SX4750 Swinging Bucket Rotor 26 Table 2 Beckman Coulter Tubes and Bottles for the SX4750 Rotor continued Adapter used in Aerosolve cannister p
8. Tube Size Adapter in Rotor mm x g Two Four grey 50 mL conical 29 x 115mm 7 28 195 6 4943 393266 393267 gt a Tube retainers and adapter plates are sold individually Continued b Do not autoclave this adapter Use cold chemical sterilization techniques only Refer to STERILIZATION AND DISINFECTION c Tube racks used with Aerosolve cannisters do not provide full tube support some manufacturers plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks Beckman Coulter highly recommends that you pretest other manufacturers tubes in the appropriate Aerosolve cannister labware using water samples 4 Cannisters and lids are made of polyphenylsulphone O rings are ethylene propylene Cannister kit includes the pad 361269 that must be used beneath the cannister Corning polypropylene bottle f Light purple adapter replaces the previous tan adapter See note under SYMMETIC AND BALANCED LOADING for weight difference information 21 SX4750 Swinging Bucket Rotor 22 Table 1 Adapters and Blood Bag Cups Available for the SX4750 Rotor continued CELL CULTURE FLASK ADAPTERS EPDM Do not autoclave this adapter Use cold chemical sterilization techniques only Refer to STERILIZATION AND DISINFECTION c Tube racks used with Aerosolve cannisters do not provide full tu
9. appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection SYMMETRICAL AND BALANCED LOADING Beckman Coulter supplies buckets and carriers in weight matched sets to make balancing easier the weight and retirement date are marked on each bucket and carrier Modular disk adapters are also sold in weight matched sets However there are variances in weight between sets as well as variance in weight between previously purchased adapters To prevent accidental imbalance it is important to keep matched sets of buckets as well as matched sets of adapters together and to weigh other adapters to be sure they are approxi mately the same Marking matched adapter sets will help you keep them together SX4750 Swinging Bucket Rotor 10 At Rest At Speed II NOTE The light purple 500 mL adapters 349945 replace the previous tan adapters there is a weight difference between the old tan adapters and the current purple adapters If you run a mix of the purple and tan adapters be sure to run matching colors in opposing positions or rotor imbalance could occur To ensure optimal performance and stability the rotor must be loaded symmetrically Two factors affect symmetric loading e The buckets or carriers must be loaded
10. cap strips between plates for speeds greater than 2700 rpm Requires soft rubber roller 4 in part number 538618 for installation e Ifthe weight of the load in a tube and bottle bucket exceeds 1000 grams or if the solution density is more than 1 2 g mL reduce the maximum allowable run speed according to the following equation reduced maximum speed 4750 rpm 1000 grams heaviest load in grams 1 27 SX4750 Swinging Bucket Rotor 28 Table 4 Commercially Available Labware for Use with Multiwell Plate Carriers Description BD Falcon 96 well flat bottom assay plate clear polystyrene standard surface nonsterile Required Accessory Part Volume Number Description Part Number 300 uL well BD Falcon 3539157 5 bag 50 case BD Falcon 96 well flat bottom ELISA plate clear polystyrene enhanced surface non sterile 300 uL well BD Falcon 353279t 25 sleeve 100 case BD Falcon 96 well flat bottom assay plate clear polystyrene standard surface nonsterile 300 uL well BD Falcon 353228t 10 bag 60 case BD Falcon is a trademark of Becton Dickinson and Company These plates can be run at 4450 rpm 4063 x g up to four plates per carrier without lids or cap strips e If the weight of the load in a multiwell plate carrier exceeds 360 grams reduce the maximum allowable run speed according to the following equation 360 grams 2
11. in a mild detergent such as Beckman Solution 555 339555 that won t damage the rotor The Rotor Cleaning Kit 339558 contains two plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water 2 Thoroughly rinse the cleaned rotor components with distilled water 3 Air dry the rotor components upside down Do not use acetone to dry the rotor Before reinstalling the rotor yoke lightly lubricate the drive hole with Spinkote 306812 to prevent the rotor from sticking SX4750 Swinging Bucket Rotor Modular Disk Adapters 1 To disassemble adapters for washing first pull the bail out of the groove in the disks then remove the disks and unsnap the bail from the rubber bottom 2 Use a mild detergent such as Solution 555 339555 diluted 10 to 1 with water and a soft brush to scrub the adapters 3 Rinse and dry then reassemble Cell Culture Flask Adapters Refer to publication GX TB 007 25 cm flask adapters or GX TB 006 75 cm flask adapters for cell culture flask adapter cleaning instructions DECONTAMINATION If aluminum rotor components become contaminated with radioactive material decontaminate them using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solu tions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking 6 and Radiacwash 7 Whi
12. materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the ultracentrifuge If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS and derate the run speed as appropriate Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use SX4750 Swinging Bucket Rotor SX4750 ROTOR nin min Tay __ fav _ Tmax Tmax __ Axis of Rotation Axis of Rotation __ SPECIFICATIONS Maximum speed Allegra X 15R tube and bottle buckets 4750 rpm Allegra X 15R multiwell plate carriers 4450 rpm Allegra X 12 series all buckets 005 3750 rpm Critical speed range 0 0 0 eee eee eee ee 580 to 720 rpm Maximum solution density 0 00000200008 1 2 g mL Relative Centrifugal Field at maximum speed using tube and bottle buckets fmax 207 8 mm 5250 x gt using multiwell plate carriers Fmax 183 2 mm 4060 x gt Conditions requiring speed reductions see SPEED DERATING Number of buckets carriers
13. rotor pins and pin sockets see Figure 5 with a paper towel then coat the sockets with Paint On Graphite Lubri cant 977212 Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge Lubrication Area both pin sockets Figure 5 Lubricating Pivot Pin Bucket Contact Areas 29 SX4750 Swinging Bucket Rotor 30 CLEANING Rotor Cleaning Kit 339558 e Refer to publication TJ6 TB 011 supplied with the cannister kit for information on Aerosolve cannister maintenance e Refer to publication GX TB 007 for information on 25 cm cell culture flask adapter maintenance or GX TB 006 for 75 cm2 flask adapters Store the rotor in a dry environment not in the centrifuge Refer to Chemical Resistances publication IN 175 for the chemical compatibilities of rotor and accessory materials Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center Wash rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently to prevent buildup of residues III NOTE Do not wash the rotor components in a dish washer Do not soak the rotor in detergent solution for long periods such as overnight 1 Wash the rotor yoke buckets bucket covers and multiwell plate carriers and covers
14. 34 3100 Internet www beckmancoulter com Telex 678413 Fax 1 800 643 4366 2008 Beckman Coulter Inc Lp Bd Printed on recycled paper
15. 61648 Tube and bottle bucket cover latch assembly 360587 SX4750u rotor assembly multiwell plate carriers 393042 Multiwell plate carrier set of 2 0 0 0 cece eee ee 392806 Multiwell plate carrier cover set of 2 0 0 00 eee eee 393071 Multiwell plate carrier cover gasket set of 4 392812 Multiwell plate carriage set of 2 0 000000000 392873 Multiwell plate support pad set of 4 000 392872 Bucket carrier cover air vent filter set of 60 368148 Pie CO wi T Dat ar mete ade Og ek elle el ee Be Si oe 369716 ACAapters osasi gi ep cece Bis races amp r RA rece eb ayn ctile Moree see Table 1 Tubes and bottles 2 0 0 0 cena see Table 2 Multiwell plate labware 00 0 0 e eee eee ee see Table 3 Soft rubber roller for aluminum foil lids 538618 Tube decanter for use with blue adapter 343108 includes gasket for 10 mm dia tubes silicone 04 343106 gasket for 12 mm dia tubes silicone 4 343107 Adapter plate 1 5 mL for use with blue adapter 354511 SX4750 Swinging Bucket Rotor Aerosolve cannister kit set of 4 each kit includes 1 cannister 1 lid 1 O ring silicone vacuum grease and 1 pad 359232 Aerosolve cannister O ring ethylene propylene 345366 Aerosolve pad placed beneath cannist
16. 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A Primegro Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za SPAIN Beckman Coulter Espafia S A C Caleruega 81 28033 Madrid Spain Telephone 34 91 3836080 Fax 34 91 3836096 email bioresearch espana beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 111 56 SE 168 11 Bromma Sweden Telephone 46 8 564 85 900 Telefax 46 8 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 0800 850 810 Fax 41 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 44 01494 441181 Fax 44 01494 447558 e mail beckman_uk beckman com Additional addresses are available at www beckmancoulter com 4300 N Harbor Boulevard Box 3100 Fullerton California 928
17. GX TB 003F April 2008 N a lt f nae N QO Bucket Rotor DH D o m a D N N D gt lt E 2 ON D lt N o on Som Q Q pA N T gt lt g Som Ey 2 lt gz S S ing m D Q F 5 9 D D DN Swing O S po ea 8 2008 Beckman Coulter Inc Printed in the U S A SX4750 Swinging Bucket Rotor Site i i ie SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equip ment is attempted When you see the symbol on other pages of this publication pay special attention to the safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate
18. ULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR RE PLACEMENT OF THE PRODUCT Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 1 905 819 1234 Fax 1 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 CZECH REPUBLIC Beckman Coulter Prague Radiova 1 102 27 Prague 10 Czech Republice Telephone 420 267 00 85 13 Fax 420 267 00 83 23 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 365 3707 Fax 41 22 365 0700 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepinte France 95942 ROISSY CDG Cedex Telephone 33 1 49 90 90 00 Fax 33 1 49 90 90 10 e mail bioresearch france beckman com
19. aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval Although rotor components and accessories made by other manufacturers may fit in the SX4750 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the SX4750 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Attach all four buckets or carriers loaded or empty to the rotor for every run Make sure that filled containers are loaded symmetrically into the rotor and that opposing containers are filled to the same level with liquid of the same density The rotor and accessories are not designed for use with
20. an damage the cannister material Aerosolve cannisters can be used as wide mouth bottles or with tubes in racks that are specially designed to fit in the cannisters Table 1 lists the Aerosolve tube racks and the number and sizes of tubes they accommodate Pads 361269 must be placed beneath the cannisters in the buckets to provide support and prevent the cannisters from being damaged during centrifugation These pads are included in the Aerosolve cannister kits 359232 Bucket covers cannot be used with Aerosolve cannisters Using Blood Bag Cups III NOTE This rotor is suitable for obtaining cell free plasma or for cell packing The yellow 90 mm cup 356856 accommodates up to two single bags or one double pack the orange 97 mm cup 356857 supports either one triple or one quad pack Do not pour liquid directly into blood bag cups Fit blood bags into cups before loading the cups into the rotor buckets Load the blood bag cups as follows SX4750 Swinging Bucket Rotor 1 Load the bags as far down into the cups as possible I gt NOTE Make sure the bags stay as vertical as possible with no folds at the top or corners If folds are present blood cells could remain in the folds and then mix with the plasma when the bag is removed 2 Sandwich the tubing between the blood bag and any transfer packs see Figure 4 Blood Bag Label Sandwich Coiled Blood Bag Seam Tubing between Ports Aligned with Transfe
21. art number 359232 c Tube retainers and adapter plates are sold individually 4 These tubes cannot be run with bucket covers in place e Do not load the 750 mL bottle directly into the bucket always use the adapter f Replacement bottle cap part number is 356263 set of 6 Adapter Volume Part Dimensions mL a Description Number Set of Two Set of Four BOTTLES continued 29 x 104 mm 50 polyallomer 357001 359474 359153 with cap assembly or 361694 359486 gt 359164 29 x104 mm 50 polyallomer 357003 359474 359153 with screw cap 359486 359164 28 5 x 107 mm 50 Teflon with high 3630764 359474 359153 speed screw cap 359486 359164 62 x 141 mm 230 conical wide mouth 3569874 356983 polycarbonate use w 349946 62 x 141 mm 230 conical wide mouth 3569894 356983 polypropylene use w 349946 62 x 136 mm 250 polycarbonate 355673 349946 with screw cap round bottom 62 x 122 mm 250 wide mouth 356013 349946 polycarbonate with cap 62 x 122 mm 250 wide mouth 356011 349946 polypropylene with cap 62 x 122 mm 250 wide mouth 358275 349946 polycarbonate 62 x 120 mm 250 wide mouth 358326 349946 polypropylene 69 x 160 mm 500 polypropylene 3556074 349945 with cap assembly 69 x 159 mm 500 polypropylene 3556654 349945 with cap 69 x 159 mm 500 polypropylene 3556504 349945 96 x 130 mm 750 polycarbonate 358299 349846 with screw cap 96 x 130 mm 750 polypropylene 356855 349846 with scr
22. ate hood or biological safety cabinet in case of tube breakage If a glass tube breaks in an Aerosolve cannister tube rack discard and replace the O ring and rubber tube cushion in the base of the rack Imbedded glass particles that remain in the bucket or adapters can break tubes during subsequent runs SST or Corvac Tubes If an SST or Corvac tube breaks the tube s silicone gel barrier material becomes laced with glass fragments and the tube contents contaminate the entire tube adapter and bucket or tube rack and cannister If one of these tubes breaks in an adapter in a bucket use the following procedure 1 Fill the bucket with water 2 Autoclave the entire bucket and contents at 121 C for 20 minutes 3 Decant any remaining water and dislodge the tube adapter do not remove it over a waste container 4 Working under a hood and using plastic gloves immerse the bucket and tube adapter intact in a beaker or similar vessel containing ethyl acetate 5 Scrub the adapter with a brush and decant the ethyl acetate mixture into a waste bottle for proper disposal 6 Rinse with water and air dry the bucket and tube adapter If one of these tubes breaks in an Aerosolve cannister use the following procedure 9 Flammability hazard Do not use in or near operating centrifuges 33 SX4750 Swinging Bucket Rotor 1 Working in a biologic safety cabinet and wearing plastic gloves open the cannister and immerse the can
23. be support some manufacturers plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks Beckman Coulter highly recommends that you pretest other manufacturers tubes in the appropriate Aerosolve cannister labware using water samples Number Tmax RCF at Flask Flasks at Flask Maximum Part Color Size per Bottom Speed Number Code cm2 Adapter mm x g qty two orange 75 1 195 3 2080 369292 green 25 2 177 5 2000 369295 BLOOD BAG CUPS polypropylene Number Tmax RCF at Bags at Cup Maximum Part Color Cup Size per Bottom Speed Number Code Capacity mm Cup mm x g qty one yellow single bag 90 2 196 2 4949 356856 double pack orange triple pack 97 1 196 7 4961 356857 quad pack AEROSOLVE CANNISTER RACKS polypropylene Nom Nom Max No Max No Tmax at RCF at Adapter Part Number Tube Tube Tubes Tubes Adapter Maximum Color Vol Dia per in Base Speed Set of Set of Code mL mm Adapter Rotor mm x g amp Two Four white 1 5 11 24 96 174 4389 354495 each blue 3 amp 5 12 24 96 174 4389 359482 359160 tan 5 12 24 96 180 4530 359489 358993 orange 10 14 18 72 175 4414 359483 359161 purple 12 16 12 48 177 4469 359484 359162 3 amp 5 12 6 24 178 4490 white 15 14 10 40 174 4389 344517 vials each a Tube retainers and adapter plates are sold individually Continued d Cannisters and lids are made of polyphenylsulphone O rings are ethylene propylene Cannister kit in
24. cal aerosols under normal conditions of the associated Beckman Coulter centrifuge when used Figure 1 The SX4750 Rotor and Accessories Corning is a trademark of Corning Incorporated 2 Validation of microbiological containment was done at an independent third party testing facility CAMR Porton Down UK or USAMRIID Ft Detrick MD U S A Improper use or maintenance may affect seal integrity and thus containment BUCKETS AND ACCESSORIES Bottle Adapter SX4750 Swinging Bucket Rotor and maintained as instructed The covers which are held in place by attached latch assemblies will contain liquids and broken tube particles reducing the need to clean the centrifuge chamber and allowing you to take appropriate precautions before opening the covers in the event of tube breakage A captive tie down device is used to secure the rotor to the drive shaft during centrifugation The centrifuge identifies the rotor during the run by means of a magnetic sensor system in the centrifuge rotor chamber and magnets imbedded in the rotor The overspeed system ensures that the rotor does not exceed its permitted speed Refer to the Warranty at the back of this manual for warranty information Several types of labware can be run in the buckets depending on your application Modular Disk Adapters Tubes are supported in modular disk adapters which can also serve as tube racks in the laboratory The adapter disks are c
25. cludes the pad 361269 that must be used beneath the cannister e Corning polypropylene bottle f Light purple adapter replaces the previous tan adapter See note under SYMMETIC AND BALANCED LOADING for weight difference information SX4750 Swinging Bucket Rotor Table 1 Adapters and Blood Bag Cups Available for the SX4750 Rotor continued AEROSOLVE CANNISTER RACKS polypropylene Nom Nom Max No Max No Tmax at RCF at Adapter Part Number Tube Tube Tubes Tubes Adapter Maximum N Color Vol Dia per in Base Speed Set of Set of Code mL mm Adapter Rotor mm x g Two Four green 15 amp 20 18 12 48 174 4389 359485 359163 3 amp 5 12 6 24 176 4439 It green 15 17 6 24 181 4565 359487 358991 conical 3 amp 5 12 6 24 180 4539 round bottom lime 50 30 4 16 181 2850 359488 358992 green conical 3 amp 5 12 4 16 180 4539 round bottom yellow 50 29 4 16 177 4464 359486 359164 3 amp 5 12 4 16 178 4490 orange 230 62 1 4 180 4534 356985 Cannister Kit4 500 183 4616 359481 359232 BOTTLE ADAPTERS polypropylene Nominal Nominal Maximum Imax at RCF at Bottle Bottle Number Bottle Maximum Adapter Color Volume Diameter Bottles Bottom Speed Part Number Code mL mm in Rotor mm x g qty one orange conical 230 62 4 195 1 4921 356983 use with 349946 yellow 250 62 4 195 1 4921 349946 warm red conical 250 62 4 203 2 5125 349849 light
26. d Aerosolve cannisters hold a variety of tube sizes in racks or they can be used as 500 mL wide mouth bottles A CAUTION Do not run chloroformed samples in Aerosolve cannisters Chloroform vapors can damage the cannister material Cell Culture Flask Adapters Specially designed EPDM ethylene propylene adapters can be used to centrifuge Corning 25 cm two per bucket or 75 cm one per bucket canted neck cell culture flasks Using these adapters the separation and concentration step of the cell culturing process can be accomplished in the flask Elimination of the need to transfer cultures into tubes for centrifugation and back into flasks minimizes the risk of contamination as well as saving time and labware 3 Validation of microbiological containment was done at an independent third party testing facility CAMR Porton Down UK or USAMRIID Ft Detrick MD U S A Improper use or maintenance may affect seal integrity and thus containment RUN PREPARATION PRERUN SAFETY CHECKS N SX4750 Swinging Bucket Rotor Multiwell Plate Carriages Used in the multiwell plate carriers to facilitate loading and unloading and to provide support to the labware during centrifuga tion each multiwell plate carriage can hold up to four stacked multi well plates not to exceed 55 9 mm 2 2 in in depth or one deep well plate Each carrier can also carry a 96 well kit for high throughput processing such as DNA or RNA kit A polyprop
27. empty modular disk adapters or water filled blood bags in cups Using Cell Culture Flask Adapters Refer to publication GX TB 007 for 25 cm flask adapter informa tion or GX TB 006 for 75 cm flask adapters These publications are supplied with the respective adapters IlIi NOTE A dry release agent is used to facilitate removal of cell culture flask adapters from buckets following centrifugation To avoid compromising the bucket cover bioseal the release agent should not be allowed on the bucket O ring When running cell culture flasks use the provided adapter to apply the dry release agent to the bucket cavity and take care to avoid contamination of the O ring Insert O ring here SX4750 Swinging Bucket Rotor Using Bucket Covers gt NOTE Because of the shape of the covers 130 mm tubes can be used only in the center cavities of the adapters when covers are used Install the cover as follows 1 Make sure that the bucket and the bucket cover surfaces are clean dry and undamaged 2 Make sure that the O ring 961648 is in good condition and lightly coated with silicone vacuum grease 335148 Place the O ring on the ledge inside the bucket 3 Place the cover on the bucket with the latches perpendicular to the pin sockets 4 Snap the latches down and secure them under the bucket rim Loading Buckets Onto the Yoke 1 Attach each bucket to the yoke by aligning the grooves in the bucket sides with t
28. er delivery of a benchtop centrifuge rotor to the original buyer by Beckman Coulter or by an authorized repre sentative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Should a Beckman Coulter cen trifuge be damaged due to a failure of a rotor covered by this warranty Beckman Coulter will supply free of charge all centrifuge parts required for repair CONDITIONS 1 Except as otherwise specifically provided herein this warranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor characteristics or operated in a Beckman Coulter centri fuge that has been improperly disassembled repaired or modified 4 Thermoplastic rotors or components used in some bench top centrifuges are warranted for one 1 year from date of purchase DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BEIN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN CO
29. er in bucket polyethylene cc eee Se SE ES ee ee 361269 Tube racks for Aerosolve cannisters 04 see Table 1 Neoprene tube cushions for blue rack pkg of 24 0 eee eee 344117 for orange rack pkg of 18 0 0 00 eee ee eee 344118 for purple rack pkg of 18 12 large 6 small 344119 for dark green rack pkg of 18 12 large 6 small 344120 for yellow rack pkg of 8 4 large 4 small 344121 Blood bag cup 90 mm yellow polypropylene 356856 Blood bag cup 97 mm orange polypropylene 356857 Balancing pads six pads 3 grams each red 358365 Cell culture flask adapter 75 cm pkg of 2 05 369292 Cell culture flask adapter 25 cm pkg of 2 05 369295 Rotor Cleaning Kit 0 00 cee eee eee 339558 Solution 555 1 qt eee es eke ee ee oe ee 339555 Silicone vacuum grease 1 OZ 0 0 nuunuu eee eee 335148 Spinkote lubricant 2 0Z 0 eee eee eee 306812 Paint On Graphite Lubricant 00 0 0 0 0000000 977212 37 SX4750 ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions in effect at the time of sale Beckman Coulter agrees to correct either by repair or at its election by replacement any defects of material or workmanship which develop within one 1 year aft
30. ew cap a Fill tubes at least half full Speed reduction may be required for fill volumes below 75 percent SX4750 Swinging Bucket Rotor Table 3 Beckman Coulter Labware for Use with Multiwell Plate Carriers Available Accessory Part Description Volume Number Description Part Number Multiwell plate 96 well nonsterile 300 uL well 609844 Cap strip nonsterile 267002 pkg 10 pkg 100 Cap strip sterile 267005 pkg 10 Aluminum foil lidt 538619 pkg 100 Deep well polystyrene plate 1 mL well 267001 Cap strip nonsterile 267002 pkg 10 96 well nonsterile pkg 24 Cap strip sterile 267005 pkg 10 Aluminum foil lidt 538619 pkg 100 Deep well polystyrene plate 1 mL well 267004 Cap strip nonsterile 267002 pkg 10 96 well sterile pkg 24 Cap strip sterile 267005 pkg 10 Aluminum foil lidt 538619 pkg 100 Deep well polypropylene plate 1 mL well 267006 Cap strip nonsterile 267002 pkg 10 96 well nonsterile pkg 24 Cap strip sterile 267005 pkg 10 Aluminum foil lidt 538619 pkg 100 Deep well polypropylene plate 1 mL well 267007 Cap strip nonsterile 267002 pkg 10 96 well sterile pkg 24 Cap strip sterile 267005 pkg 10 Aluminum foil lidt 538619 pkg 100 Square well polypropylene plate 2 mL well 140504 Aluminum foil lidt 538619 pkg 100 pkg 24 Caps are optional however if stacking two or three multiwell plates use
31. he pivot pins then sliding the buckets down until the pivot pins are seated in the bucket pockets A CAUTION Attach all four buckets loaded or empty to the rotor yoke If only two buckets are filled place them in opposite positions on the yoke All four positions must contain buckets or carriers during a run Never run the rotor with only two positions filled 2 Gently swing the buckets to ensure that they are properly seated on the pivot pins 17 SX4750 Swinging Bucket Rotor USING MULTIWELL PLATE CARRIERS If only two filled multiwell plate carriers are run install them oppo site each other in the rotor and run two additional carriers or buckets they can be empty to prevent rotor imbalance 1 Insert the filled plate s into the carriage If using two or three Beckman Coulter plates per carrier place a 96 well cap strip see Table 3 between the plates to prevent breakage during centrifugation gt NOTE Up to three Beckman Coulter plates separated by cap strips can be run per carrier at maximum multiwell plate speeds If running four Beckman Coulter plates cap strips cannot be used due to height limitations reduce run speed to 2700 rpm Observe manufacturer s run speed and stacking limitations if using other manufacturers multiwell plates RCF and stacking limitations vary widely between manufacturers pretest other manufacturers plates using water instead of valuable sample 2
32. labware from the buckets or carriers 3 If removing the rotor yoke turn the tie down T bar to the left counterclockwise to loosen it Lift the yoke straight up and off the drive shaft 19 SX4750 Swinging Bucket Rotor 20 TUBES BOTTLES AND ACCESSORIES 25 C 2 C lw tT RUN SPEEDS The rotor uses labware listed in Tables 1 through 3 RCF limitations for some non Beckman Coulter multiwell plates are shown in Table 4 Temperature Limits e Beckman Coulter plastic tubes and bottles have been centrifuge tested for use at temperatures between 2 and 25 C For centrifuga tion at other temperatures pretest tubes under anticipated run conditions e If plastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation Labware Beckman Coulter tubes and bottles listed in Table 2 can be used in tube and bottle buckets Refer to Table 3 for Beckman Coulter labware that can be centrifuged in multiwell plate carriers The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields rcf When rotational speed is adjusted so that identical samples are subjected to the same rcf in two different rotors the samples are subjected to the same force The rcf at each speed is automatically calculated by the centri fuge software if the rcf is entered the cen
33. le Beckman Coulter has tested these materials and found that they do not damage components no guarantee of decontamination is expressed or implied Follow appropriate decontamination proce dures as directed by your laboratory safety officer V X If the rotor or other components are contaminated with toxic or patho genic materials follow appropriate decontamination procedures as directed by your laboratory safety officer 6 In U S A contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 7 In U S A contact Biodex Medical Systems Shirley NY internationally contact the U S office to find the dealer nearest you 31 SX4750 Swinging Bucket Rotor 32 STERILIZATION AND DISINFECTION 121 C D Qmm TUBE BREAKAGE e The rotor yoke buckets adapters and multiwell plate carriers can be autoclaved at 121 C for up to an hour Blood bag cups bucket and carrier covers and Aerosolve cannisters can be auto claved at that temperature for about 15 minutes Do not autoclave Aerosolve cannisters purchased prior to April 1993 or any components made of Noryl Remove the covers from the buckets and place the rotor yoke buckets covers and or multiwell plate carriers in the autoclave upside down Before autoclaving remove the air vent filter from each cover b
34. lightly lubricate the drive hole with a lubricant such as Spinkote 306812 AN CAUTION Never drop the rotor yoke onto the centrifuge drive shaft The drive shaft can be damaged if the rotor is forced sideways or dropped onto it 2 Carefully lower the yoke straight down onto the centrifuge drive shaft Be sure the yoke is properly seated on the shaft 3 Turn the tie down T bar to the right clockwise to tighten the yoke firmly on the drive shaft I gt NOTE If the rotor yoke is left in the centrifuge between runs before each run make sure it is properly seated on the drive shaft and that the T bar is tight SX4750 Swinging Bucket Rotor LOADING THE BUCKETS For runs at other than room temperature refrigerate or warm the rotor and precool the centrifuge beforehand for fast equilibration AN WARNING Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infec tious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or other hazardous materials in this rotor with out taking all
35. load buckets before or after they are installed on the rotor yoke In either case we recommend filling the appropriate labware first and then loading the labware into the buckets Using Tubes Assembling Modular Disk Adapters Refer to Table 1 to determine which color adapter is required for the tubes you are using and the number of disks needed to support the length of the tubes 1 Select the appropriate adapter base from Table 1 and attach a bail to it see Figure 3 2 Position one of the disks so that its grooves are aligned with the bail Push the disk down until the bail snaps into the grooves 3 Add more disks in this way until the height of the assembly is nearly as tall as the tubes you will be using If the height of the disks is very tall you may have to push the bail into the grooves of the top disks by hand Remove or add disks to the bail to accom modate shorter or longer tubes If the tubes fit too snugly in the adapter s rubber base apply a light film of powder such as talcum powder to prevent the tubes from sticking Loading Tubes in Adapters Loading Adapters in the Buckets SX4750 Swinging Bucket Rotor Figure 3 Assembling a Modular Disk Adapter When placing tubes in modular disk adapters it is important to make sure that they will not contact the rotor yoke during a run e One way to do this is to place empty tubes in an adapter place the adapter in a bucket on the rotor yoke and manually s
36. nister lid and contents intact in a beaker or similar vessel containing 6 sodium hypochlorite undiluted bleach 2 Leave the cannister to soak overnight 3 Carefully dislodge the tube rack and scrub it with a brush 4 Decant the bleach into a waste bottle for proper disposal 5 Rinse with water and air dry the cannister and tube rack STORAGE When it is not in use store the rotor in a dry environment not in the centrifuge 34 RETURNING A ROTOR SX4750 Swinging Bucket Rotor Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form which may be obtained from your local Beckman Coulter sales office should contain the following information e rotor serial number e history of use approximate frequency of use e reason for the return e original purchase order number billing number and shipping number if possible e name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and e name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such a
37. olor coded by the tube size they accommodate see Table 1 the number of disks used in an adapter assembly depends upon the length of tubes used A tube decanter is available to hold either 10 mm or 12 mm tubes securely in the blue adapter allowing all the tubes to be decanted at once Addi tionally 1 5 mL Microfuge tubes can be run using a special plate that fits on top of the blue adapter Both of these accessories are described in Table 1 Beckman Coulter tubes and bottles available for use in the rotor buckets are described in Table 2 Bottle Adapters Bottles are supported in polypropylene adapters that fit inside the rotor buckets The adapters are ribbed for strength and accommodate three bottle sizes including one conical bottle see Table 1 SX4750 Swinging Bucket Rotor q_ gt Y Aerosolve Cannister 75 cm2 Flask Adapter Blood Bag Cups Polypropylene cups provide support for blood bags in the rotor buckets Blood bag cups are available in two sizes one for single or double pack bags and one for triple or quad pack bags see Table 1 Aerosolve Cannisters Aerosolve cannisters designed to contain aerosol leakage and liquid spills can be used in the SX4750 buckets when this additional containment is required The cannister was tested to demonstrate containment of microbiological aerosols under normal operating conditions of the associated Beckman Coulter centrifuge when used and maintained as instructe
38. purple 500 70 4 200 2 6050 349945 blue 750 96 4 195 2 4921 349846 a Tube retainers and adapter plates are sold individually b Do not autoclave this adapter Use cold chemical sterilization techniques only Refer to STERILIZATION AND DISINFECTION Tube racks used with Aerosolve cannisters do not provide full tube support some manufacturers plastic and glass tubes cannot withstand the maximum forces generated by this rotor when used in these racks Beckman Coulter highly recommends that you pretest other manufacturers tubes in the appropriate Aerosolve cannister labware using water samples 4 Cannisters and lids are made of polyphenylsulphone O rings are ethylene propylene Cannister kit includes the pad 361269 that must be used beneath the cannister Corning polypropylene bottle f Light purple adapter replaces the previous tan adapter See note under SYMMETIC AND BALANCED LOADING for weight difference information 23 SX4750 Swinging Bucket Rotor 24 Table 2 Beckman Coulter Tubes and Bottles for the SX4750 Rotor Adapter used in Aerosolve cannister part number 359232 Tube retainers and adapter plates are sold individually d These tubes cannot be run with bucket covers in place e Do not load the 750 mL bottle directly into the bucket always use the adapter f Replacement bottle cap part number is 356263
39. r Bags Pivotal Axis of and Filled Bucket Blood Bag N _ Blood Bag Seam Blood Bag Label Blood Bag Label Blood Bag Label Blood Bag C Blood Bag Label ogee Up Figure 4 Blood Bag Loading Procedures 3 Make sure the loaded blood bag cups opposite each other on the rotor yoke are approximately the same weight within 1 gram Soft pliable balancing pads that will not puncture blood bags see the SUPPLY LIST can be used to maintain weight balance if necessary 4 Place loaded cups into rotor buckets To reduce the possibility of bag breakage align the blood bag seam with the rotor pivot pins see Figure 4 with the label facing out 15 SX4750 Swinging Bucket Rotor 16 5 Place bucket covers on rotor buckets to prevent blood bags or tubing from contacting the rotor yoke during centrifugation A CAUTION If bucket covers are not used make sure the superstructure of the blood bag protruding from the cup does not inhibit the bucket from reaching its horizontal position If it does remove the cup from the rotor and reposition the blood bag so that it seats further into the cup Allowing the blood bags to contact the rotor yoke during centrifugation can cause the bucket to come off the pivot pins and can seriously damage both the rotor and the centrifuge If only two filled cups are run place them in opposing buckets The other two buckets should contain similar blank loads to prevent imbalance either
40. s tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office 35 SX4750 Swinging Bucket Rotor 36 SUPPLY LIST I gt NOTE Publications referenced in this manual can be obtained by calling Beckman Coulter at 1 800 742 2345 in the United States or by contacting your local Beckman Coulter office Contact Beckman Coulter Sales 1 800 742 2345 in the United States worldwide offices are listed on the back cover of this manual for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS SUPPLIES SX4750 rotor assembly tube and bottle buckets 369702 Tube and bottle bucket set of 2 20 0 cece ee 392804 Tube and bottle bucket cover set of 2 0 0 0 0 0 e eee eee 392805 Tube and bottle bucket O ring pkg 8 0005 9
41. set of 6 Adapter Volume Part Dimensions mL Description Number Set of Two Set of Four OPEN TOP TUBES 16 x 76mm 10 polyallomer 355640 349471 359150 359484 359162 16 x 76 mm 10 polycarbonate 355630 359471 359150 359484 359162 16 x 76mm 10 stainless steel 301108 359471 359150 359484 359162 18 x 98 mm 15 polycarbonate 342080 359473 359152 18 x 98mm 15 polyethylene 342081 359473 359152 18 x 98 mm 15 polypropylene 342082 359473 359152 29 x 104 mm 50 polycarbonate 363075 359474 359153 graduated pkg 8 359486 gt 359164 29 x 103 mm 50 polypropylene 357007 359474 359153 359486 359164 29 x 103 mm 50 polycarbonate 363647 359474 359153 359486 359164 TUBES WITH SNAP ON CAPS 11 x 38 mm 1 5 polyallomer 357448 359469 359148 354511 354495 11 x 38 mm 1 5 polypropylene 356090 359469 359148 354511 354495 11 x 38mm 1 5 blue 356091 359469 359148 polypropylene 354511 354495 11 x 38 mm 1 5 green 356092 359469 359148 polypropylene 354511 354495 11 x 38mm 1 5 yellow 356093 359469 359148 polypropylene 354511 354495 a Fill tubes at least half full Speed reduction may be required for fill volumes below 75 percent Continued SX4750 Swinging Bucket Rotor Table 2 Beckman Coulter Tubes and Bottles for the SX4750 Rotor continued Adapter Volume Part Dimensions mL Description Number Set of Two Set of Four TUBES WITH
42. symmetrically with respect to their pivotal axes e The rotor should be loaded symmetrically with respect to its center of rotation For best results load opposing buckets or carriers with the same type of labware containing the same amounts of fluid of equal density Additionally opposing buckets or carriers and their contents should weigh approximately the same within 6 grams Do not exceed the rated maximum load for buckets 1000 grams each for tube and bottle buckets 360 grams each for multiwell plate carriers It is not necessary to completely fill all tubes or positions in buckets however partially filled buckets must be balanced with respect to the bucket pivotal axis see Figure 2 In multitube adapters each tube should be placed so that its weight is balanced by a tube in a diametrically opposite position across the pivotal axis in the same adapter Adapters placed in opposing buckets should also be filled the same way In multiwell plate carriers samples should be loaded into the wells symmetrically with respect to the pivotal axis of the bucket and opposing carriers should contain similar loads 4 Fill tubes at least half full Speed reduction may be required for fill volumes below 75 percent SX4750 Swinging Bucket Rotor Balanced Load Unbalanced Load Pivotal Axis of Bucket Center of Rotation CORRECT C 0000 C 0000 Pivotal Axis e0 00000000000 O O Q O O O
43. trifuge calculates the equivalent rpm revolutions per minute Run speeds must be reduced in the following circumstances SX4750 Swinging Bucket Rotor Table 1 Adapters and Blood Bag Cups Available for the SX4750 Rotor MODULAR DISK ADAPTERS polypropylene Nom Nom Max No Max No Imax at RCF at No Adapter Part No Tube Tube Tubes Tubes Adapter Maximum Disks Color Vol Dia per in Bottom Speed per Set of Set of Code mL mm Adapter Rotor mm x g Adapter Two Four blue 3 10 37 148 184 7 4659 5 359469 359148 5 12 tan 3 amp 13 30 120 184 7 4659 5 359478 359157 5 orange 7 amp 14 24 96 184 7 4659 6 359470 359149 10 purple 12 16 19 76 184 7 4659 7 359471 359150 green 15 18 14 56 194 7 4911 6 359472 359151 conical green 15 amp 18 14 56 184 7 4659 7 359473 359152 20 It green 30 amp 30 4 16 191 4817 5 359475 359154 conical 50 yellow 50 29 7 28 184 7 4659 6 359474 359153 dk blue 50 35 4 16 184 7 4659 7 359476 359155 brown 100 44 2 8 184 7 4659 3 359477 359156 tube 3 10 37 148 1 343108 decanter 5 12 each 1 5 mL 1 5 amp 11 26 104 1 3545114 adapter 1 8 each plate SOLID ADAPTER polypropylene Maximum Adapter Part No Number Maximum max at RCF at of Tubes Number Adapter Maximum Color Nominal Nominal per of Tubes Bottom Speed Set of Set of Code Tube Volume
44. wing the bucket to the horizontal position making sure that all tubes clear the yoke e Another method is to place the adapter in a bucket and position a bucket cover over the tubes checking that tubes do not touch the cover If all positions in an adapter are not filled load the adapter symmetri cally with respect to its pivotal axis see SYMMETRICAL AND BALANCED LOADING above After placing tubes in the disk adapters lower the adapters into the buckets so that the bails line up with the rotor pivot pins If only two loaded adapters are run place them in opposing buckets The other two buckets should contain empty modular disk adapters do not centrifuge empty cell culture flask adapters to prevent imbalance See SYMMETRICAL AND BALANCED LOADING above 13 SX4750 Swinging Bucket Rotor 14 Aerosolve Tube Rack Using Bottles in Adapters Load bottles into their appropriate adapters see Table 1 then place the filled adapters in the rotor buckets If only two bottles are run place them in opposite buckets Make sure the other two buckets contain at least a minimal blank load such as empty modular disk adapters to prevent rotor imbalance If modular disk adapters are not available use two water filled bottles in adapters to balance the load See SYMMETRICAL AND BALANCED LOADING above Using Aerosolve Cannisters A CAUTION Do not run chloroformed samples in Aerosolve cannisters Chloroform vapors c
45. y gently pushing it out from underneath the cover with a pencil or other non metal tool that will not scratch the cover material After autoclaving insert a new air vent filter 368148 into each cover e Ethanol 70 8 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use Refer to publication IN 192 included with each box of tubes for tube and bottle sterilization and disinfection procedures A CAUTION To reduce the potential for corrosion clean buckets or carriers thoroughly immediately following a tube or well plate breakage If a glass tube breaks remove the glass very carefully from the adapter and bucket Imbedded glass particles that remain in the bucket or adapters can cause tube failure during subsequent runs 8 Flammability hazard Do not use in or near operating centrifuges SX4750 Swinging Bucket Rotor Aerosolve Cannisters AN WARNING When working with potentially hazardous materials open cannisters in an appropri
46. ylene pad provides support for the labware Specific information about the SX4750 rotor is given here Informa tion about the use and care of the centrifuge is contained in the centrifuge manual which should be used together with this manual for complete rotor and centrifuge operation gt NOTE Although rotor components and accessories made by other manufacturers may fit in the SX4750 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the SX4750 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Read the SAFETY NOTICE page at the front of this manual before using the rotor 1 Make sure that the rotor yoke buckets and or carriers and covers are clean and show no signs of corrosion or cracking If any evidence of damage is present do not centrifuge the rotor SX4750 Swinging Bucket Rotor 2 Check the chemical compatibilities of all materials used Refer to Chemical Resistances publication IN 175 3 Verify that the tubes bottles and accessories being used are listed in Tables 1 through 3 INSTALLING THE ROTOR YOKE gt NOTE Before the first use of a new rotor attach the tie down T bar 369716 to the rotor yoke Screw it down as far as possible 1 Before installing the yoke in the centrifuge
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