Home

ACQUIRE 4.2

1. Note The Program Launcher is currently available for Windows 98 2000 ME only To start ACQUIRE under NT4 0 click on the Start button followed by the Scan 4 program group and then Acquire If you purchased a system with 64 or fewer channels the top line of the display will show Acquire 64 channels Ifyou have a 128 channel system that will be indicated on the top line This let s you see the largestnumber of channels that your system may record As part of your initial SCAN 4 2 installation your amplifier and A D card settings should be entered The software settings will vary depending on which A D card is installed and proper installation requires some in depth knowledge of your computer you should make a note of the settings in case you ever need to re enter them in the future If you have not already completed this section please refer to the Installation manual or the beginning of the Operating ACQUIRE section below for more information Constructing a setup file This section describes several configurations of ACQUIRE The intent is to show you how quickly to configure ACQUIRE for your particular application These applications will be presented in a tutorial format Explanation of particular commands and settings will be brief Please refer to the Operating ACQUIRE section below for a complete description of all features of this module The following configura tions will be presented for typical EEG and evoked potenti
2. mg Wat mm batteren ia a ee pedal A atb tte aa me gedu py dans Ei ET ES L li e b Ch KC Lat Aen ame ee ee Bag tem Ap a E Vd P Oh JE M iy CPE ah F at PF nage ri Aree a tao Sena tga ll e ir PoP apn cathy d A la ANE ge ag Bs e veer wi ele egent Stach WE tt o aaan laacht felt Meta P Ra W wien Det em bi n ba ayh cae oy mm Be tg Mi at BE rt i ETS ee al Ee ae ee gd one eee ee ee ee ee ee a TE IR et erg i ee i sr ed ee EL eg p Ae a E E ay Mer Tiii FE jei alt Ma LA FE ch a T m Ee pei gl py pee ke nn ai i a eg ei den ain ET A ee elle oai anais apes ee e om eg rm bere enn eee Walt aiaia a i misten ap d d bm et laiia a es z L em FEI E Be LEI z a ni gh bgp p e ee e eT ppe es a h i a e LT Aen gl ge i a aa ee zur D d a e aem p E a p e RE Ae de A p amina a Ta ES FEI geck gett dert et ey a ae a ae a ec fly EH P brai i DN aa Ea p a d a hT e i aa arg Tra Eir ci a RO pe pa ee i i d a i i I J Eat Had bes Fi Plage A Status box will show the percentage of buffer space that is being used the amount of free space on your hard drive the number of sweeps accepted the number of sweeps rejected and the trigger type code of the last sweep etc see Operating ACQUIRE below for more details Refer to the View section in the Operating ACQUIRE details below for description of the Toolbar options for controlling acquisition Termination will occur after the 200 sweeps are accepted
3. ACQUIRE4 2 142 Copyright 2001 Neurosoft Inc Document number 2202 Revision B At the bottom left there is a Store as Default button that may be useful for example if you are obtaining multiple recordings over time from the same subject or if there are many fields that contain the same information for all subjects When you have entered the information click on the Save button on the right and a Windows Save File utility will appear Give the file a name and the sub extension will be added automati cally The information that you save will be included in the header file and will be accessible from the EDIT module To recall a saved sub file click on the Load button An Open File utility will appear allowing you to select the desired file To cancel the information in the fields and prepare for new information to be entered click on the Clear Form button When you are finished click OK to continue Click Cancel to exit the dialog box SynAmps Hardware NuAmps Metrabyte card or Labmaster Card Depending upon which amplifiers or A D card you installed you will see a corresponding option in the Edit menu screen It will either have SynAmps Hardware NuAmps Hardware Metrabyte Edit View Acquisition Options Overall Parameters Channel Layout Channel Assignment Table Make default setup Subject Spnamps Hardware DAS 1800 Amplifier or Lab Master A D Amplifier options Ifnone of these opt
4. Step 3 Click Edit from the Main menu bar then click Overall Parameters Overall Parameters A multi card window will appear with the Startup card on top For this recording enable the Multiple ACQUIRE4 2 29 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Single Window Display No 1 Enable FilterDisplay Derivation Derivation File Single Window Display No 2 AA Enable P Eiter misoa 7 Genvaton Mensatamiie Multiple Window Display nied Oy act a aa Age Aye If Enable Filter Display Derivation Ueryation File Sorted Average Window Display Spectral Display M Enable d Enable Negative C Up SerMepar alter T Display this Menu on Startup D Auto Save Window Display and Sorted Average Window Display click on field so that check marks appear Set the polarity as desired Negative Up or Down Make sure no other displays are enabled Step 4 Now click the Amplifiers tab Set the A D rate to 10000Hz SynAmps users toggle to this value For non SynAmps systems enter 2000 Next enter 4 for the number of channels corresponding to electrode placements we will set for C3 CH C4 and C4 Click the Reset Positions button and you will see 4 electrode displays numbered 1 4 Don t worry about the labels or positions at this point we will modify them shortly these have already been renamed and repositioned in the figure below For Acquisition type select Epoched This
5. Note Do NOT interrupt the downloading process or else the programs may become corrupted rendering the SynAmps unbootable It is recommended that you disable your Screen Saver and any other Power Management options that are enabled in Windows If there is a problem with transmission over the SCSI cable turn the PC and SynAmps off then attach the serial cable supplied with the SynAmps and reboot SynAmps first Then on the Update Drivers screen select the Serial cable and select either Com 1 or Com 2 depending on where you have the serial cable Then try the update procedure again If this fails also contact NeuroScan technical support ACQUIRE4 2 68 Copyright 2001 Neurosoft Inc Document number 2202 Revision B In the Diagnostics there is another option called Test Trigger Port Clicking it displays the Trigger port test window This is useful for testing the triggers coming from STIM or other stimulus presentation system Trigger port test Decimal Binary Stimulus o 00000000 Last Stim o jecoono00 Response JD 0000000 Counter 0 HoldWalue 0 Turn on the STIM system go to the AUDCPT program for example and select the P300 demo file Note verify that the Port Address is set correctly in the P300 setup file This should be 848 for new SoundBlaster systems and 1820 for the older LabMaster systems Ifthe system 1s set up properly you should see the following Start the P300 program listen for the beeps t
6. The digitization rate of 1000Hz will provide enough resoluion to determine peak latency and amplitude of the major components Leave the number of channels at 32 For Acquisition type select Average from the pull down menu to create an online average evoked potential Ifyou are using SynAmps under AC DC select DC Correction enable the Enable field so that the check mark appears with a 70 correction value If you are not using SynAmps this section will not be accessible Under Amplifier Settings select a Gain of 1000x set the Low Pass filter for 100Hz and the High Pass filter to 1Hz for non SynAmps systems the gain and filter settings are set in the Metrabyte or LabMaster option under Edit from the Main menu bar and should not be changed unless you physically reset the amplifiers and calibrate with the new settings Leave the Notch filter OFF no check mark in the field Syn Amps only Thenclickthe SelectAll button all electrode labels will turn green and click the c Mi GE S button to apply the settings you have entered to all channels This may take a few seconds To verify your settings click on an electrode label once The label will appear in the Selected Channel KG field and the applied settings will appear in the Gain and Filter fields Note you cannot assign different AD rates to individual channels all channels must have the same AD rate Step 5 Next click the Channel Attributes tab These options allow
7. General Sweeps li UU Start mg 100 Display Start rng 100 Artifact Rejection W Enable Start ms E OU End me 1455 Stop msz 439 End ma 1433 Y Min 100 Fonts BUC Avg Update f Mm p Lon May p 50 Y Mag 100 Y Autoseale Baseline Correction W Enable Start ms E DU End mz lo ACQUIRE4 2 111 Copyright 2001 Neurosoft Inc Document number 2202 Revision B NOTE There has been one notable change in SCAN 4 2 which is to make the data point count more accurate For example in prior versions if you had an AD rate of 1000 and epoch spans from 100 to 900ms the program would show that you have 1000 data points one per ms In reality there are 1001 data points when you include the starting point the ending point and the zero point In 4 2 all points are counted In the 4 2 Epoching transform for example let s say you have an AD rate of 250 and you wish to create epochs from 0 to 1000ms You will find that there are now 251 points rather than 250 points in the prior versions If you wish to create epochs with a power of 2 number of points you should go by the points field 512 not the Start Stop times Does this mean you cannot combine your old data files with new ones No Your old data files will be read in the new way For example retrieve the vep avg file in an prior version of EDIT and it will show an epoch span from 50 to 500ms Retrieve the same
8. You may create up to 10 different averages in which you may sort by types of stimuli and of responses Startup Amplifiers Channel Attributes Triggers Epochs Fsp Average Frequency Sorting Audio Map Criteria M Enable Online Averaging FREG lt lt Add Average M Stimulus 200 IT Response gt gt Del Average Parameters Sweep FOO Baseline Correction Enable Sharh mel BS Stamimel E Sort file Export Import Artifact Rejection IT Enable Stele me E UU Stop me i UU Jam Lon Marmi ball Display Te Autoscale Start ris 100 Stop mz D OOD Start ma 100 Stop mz li ogg 4 Min p 1 100 May pi li Oo Points AAT Avg Update li You must first specify the average files that will be created Click on lt lt Add Average and a Windows Save File utility will appear Enter a file name the avg extension will be added automatically You will see the file names being added to the top left hand space in the display If you wish to remove a file name highlight it and click the gt gt Del Average button You will likely have more than one sorted file To set up multiple files set all the criteria that you wish for the first file including the criteria that you will be using for the sort trials stimulus response Enter the type codes explained below for the first sorted file Then click the lt lt Add Average button to create the next
9. 18 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Channel 1 32 22 mmm oCh mmm mmm mm mmm Label Label Label r 7 j co Ph La Ph FT E 10 CG H oo HTT IHN VO i jik a n T hd NS 0z 11 ch H LI l DJ hi IW l DJ 3 DJ it KA esch KA hh LD KA LD l F 13 CP4 14 fou PZ Ps KA A l a CO LC GO TI 7 esch du F 15 C 16 GA FLA KA mo KA DJ CO ho L DJ IL m mm GO IK E Fb cl D MJ DJ The order of the channels is contingent upon your actual cable connections and amplifiers refer to your physical installation manual for more details as well as the Channel Assignment table section in the Operat ing ACQUIRE section below Click OK to continue Step 11 If you have not already Saved the Setup file by clicking Save Setup under the File option from the Main menu bar use VEPSET again to add the changes to the Setup file Step 12 To begin viewing click Acquisition on the Main Menu bar followed by Start Acquisition or just click on the green triangle toward the left end of the Toolbar gt If the Toolbar is not displayed enable it by clicking on the Toolbar line under View on the Main Menu bar You will be asked for a file name for the AVG file and the program will then wait for triggers from STIM or other similar system In the online wi
10. Band Pass filtering passes frequencies within the Low and High pass settings and attenu ates frequencies outside of this range Clicking this option activates the High Hz and Low Hz fields in the Band Pass section below Band Stop filtering passes frequencies outside the Low and High pass settings and attenu ates frequencies within this range Clicking this option activates the Start Hz and Stop Hz fields in the Band Stop section below Rectify You may select whether or not to Rectify the waveforms prior to filtering This option is implemented primarily for EMG recordings and is useful for identifying the onset of an EMG burst Channels Notice that in this display as opposed to the other similarly appearing displays Amplifi ers and Channel Attributes there is an Jk Enable button Individual channels may be selected for filtering although the steps are slightly differnent than selecting channels in the other screens The basic steps are to 1 select the channels to be modified 2 enter the desired settings and 3 apply the settings to the selected channels The order matters in this instance ACQUIRE4 2 83 Copyright 2001 Neurosoft Inc Document number 2202 Revision B For example retrieve the Cap32 ast file and go ro the Filter display All channels are set for Low Pass filtering at 30Hz 12dB oct All of the channels are selected by default green Let s say we want to change the filtering on the VEOGL and HE
11. Click the SE Ze kenn A Save As utility window will appear in which you may specify the path and enter a file name dat extension added automatically View the file using a text editor such as Notepad Wordpad etc The calibration values can also be saved offline in EDIT Stop Clicking onthe Stop Stop line terminates storage of information and closes the Single and Multiple Window Displays Restart Clicking thisline Festat allows you to restart data acquisition For example if you have begun recording data and an interruption occurs you can restart acquisition with this option You will be prompted to re enter the file names of files to be saved This feature may be accessed directly from the Toolbar icon 2 Record This option serves tha same function as the Record icon from the Toolbar Please refer to that section for more details ACQUIRE4 2 164 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Pause Display This option serves tha same function as the Pause icon 11 from the Toolbar Please refer to that section for more details Annotation Clicking on this option allows you to add text remarks at any point in the continuous recording For example if you wish to make a note about extraneous lab noise at a certain point in the recording click immediately on the annotation line or preferrably the Type annotation button on the Toolbar A A window will appear in which you may type whatever
12. T arala LECECEM d 100 285 Cancel i From this screen you may edit the electrode label and modify the Polar and Cartesian coordinates manually if desired Click OK to apply the changes Ifyou change the Polar coordinates clickthe Polar to Cartesian button to modify the Cartesian coordinates automatically and vice versa Electrodes can be repositioned manually on the display You can place them at any point or you can have them snap to defined positions The latter option is enabled withthe M Snap tagid field You can control the sensitivity ofthe snapping by the values you enterin the Theta and Phi fields Thetali0 lg Valid entries for these fields are from 01 to 10 degrees When theta is set to 10 for example the moving electrode will snap from one position to the next in discrete 10 degree steps Similarly when phiis set for 10 the moving electrode will snap from one position to the next 1n discrete 10 degree steps Ifthe Snap to grid field isnot checked you can place the electrode at any position The remaining options on the display are as follows ACQUIRE4 2 137 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 138 Match Labels Cartesian to Polar Load 3D pos Polar to Cartesian The Match Labels button is used to position the electrodes according to the 10 20 placement system This assumes you are using conventional electrode labels The program
13. The triggering category determines how sweeps are to be initiated during acquisition There are three types of triggers Startup Amplifiers Channel Attributes Triggers GG External Le Hold value lo IT Use inverted values Trtental terval 2 oz C Yoltage homme TE 7 Threshold ms i OU Slope fe Weg E beet ACQUIRE4 2 109 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 110 External selection field With External Triggering all acquisition systems sweeps are initiated by an external device such as STIM Click on the circular field to select External triggering Hold Value The Hold bit pattern value determines what value of the signal at the trigger port will initiate a sweep a value other than the hold value will trigger acquisition Ifa sweep is initiated by the number 255 bits 0 7 all high for example the number will 255 appear in the Last Type field of the Status box Hold values are applicable to External 8 line parallel port triggering as with the STIM system The threshold for triggering will typically be zero if you are triggering from the STIM audio interface port Trigger types may range between and 255 Different machines and setups may require different values 1n the threshold field Ifyou specify what you believe to be the correct hold value for your machine but the program seems to be acquiring sweeps of data spontane ously there is an easy w
14. Web www neuro co ACQUIRE4 2 2 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Table of Contents TRAE ACQUIRE un eil EE 8 FAO OUT IMS TE DEE 9 San ACQUIRE EE 9 C nstucinga UE 11 Online averadihg V EE 12 Single sweep recording and online sorting H 200 20 Single sweep recording and an online average GER 28 Interfacing with an External SEP G mulator 32 Triggering from Sound ENNEN 33 Triggering from the SEP Stimulator 0000nnnnne0000aaaaan 34 Re G AAB Rosiren ER E EE EEEE E 39 Continuous recording online EMG audio outout and online filtering NAOU ccc eeeesssssssssssssessesseeessseeeeeeeaeeees 47 Continuous recording and online frequency analysis and topographical mapping spontaneous EEG 54 Operating ACQUIRE EE 65 Installation of Amplifiers and A D Card 65 SVAMM araa e 67 PAGO G E 67 Head leese 67 lev e TT 67 NUAMD Sannaa a ER E peeaanminaditaamneaines 69 NUAMPS seiup Tee a 70 ue lee EE 70 NUAMPS setpecreen EE 70 RECENO EE 71 Bipolar recordmgs 11 TAG GG TE 72 leet DEE 72 IDASA1G0Z EE ME 73 FAMINE neue gege Deag tee 13 Trigger Out voltage EN 73 A D Converter Hange ENNEN 74 Lezdlelfwelfure IO 74 AD MASON TEE 14 AMPIE geed eege Eed 15 Tigger QUE Volagere S 15 A D Converter RAnge ccccccscccccseeseeeeesesssssssssssssseaees 76 VO CIR PUY OQIDUD ME 76 TNE ACQUIRE POO a eege Scanian EE stantadeshiuctd chads cent deseeasseheieatenesacanensc 77 SUCKY PINS EE 77 Eege 7
15. button to add the information to the workspace file then click on OK to continue Click on Cancel to leave the page without invoking any of the selections General The General page is divided into Colors and Misc options ACQUIRE4 2 167 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 168 X Anie Y Axis General Misc W Waveform Bos Colors T Overlay Waveforms i Display Electrode Labels Time Zero h Show Standard Deviation Waveform M Show Head Overlay waveform Bkgnrid window Bkgnd Waveform Bos E C From file Cursor Negative Up Polarity Frequency Bands Ge Negative Down Colors Under the Colors section you may specify the color for various display features including Text Axes Time Zero Waveform Frequency Bands Waveform Background Waveform Box and Window Background Selecting any of these except the Frequency Bands option will display the basic color palette consisting of 48 options Click on the desired color then click OK to invoke the color Note The Text color will determine the color of the electrode labels When selecting the Text color bear in mind that Bad channel labels will always appear in red and Skipped channel labels will always appear in black regardless of the color you select for the Text If you would like to create your own custom color click on the Define Custom Colors
16. li EOG Fpl Phys Chn T who fu Gu nu fis Label FTS FL FLA TP Phys Chn che fis fis Label Phys Chn Label Channels are assigned to the lowest numbered inputs and are given the lowest channel assignments one to one mapping Channels can be reassigned with all A D systems On SynAmps based systems channels can be reassigned within a bank of 32 channels For example if you are record ing 10 channels the first 8 physical channels and the 29th and 30th physical channels enter 10 for the number of channels under Amplifiers and relabel the Physical Channels 9 and 10 as 29 and 30 respectively Click OK to apply any changes you have made or CANCEL to leave the screen without applying any changes Remember to SAVE the setup file for future applications use Save Setup under File Make default setup The Make default setup option replaces the settings in the current Setup file with those in the supplied default file Click on the Make default setup line under Edit on the Main menu bar and Edit View Acquisition Options Overall Parameters Channel Layout Channel 4ssignment Table Make default setup Subject Synamps Hardware you will get a warning saying that you are about to replace the current settings with the default ones ACQUIRE AN Wearing about return current setup to default values Cancel ACQUIRE4 2 141 Copyright 2001 Neur
17. or after user interrupt by clicking on the Stop icon on the Toolbar Single sweep recording and online sorting P300 This section describes how to configure ACQUIRE for single sweep epoch acquisition and how to per form online sorting of different types of stimuli This option is particularly useful for monitoring both the acquisition of the average and the reconstruction of the average offline with the EDIT module Artifactual sweeps that may not have been removed in the online average can be removed or corrected offline to minimize their effects on the reconstructed average To illustrate this type of data acquisition the system will be configured to acquire a simple odd ball auditory P300 evoked potential Follow these steps to configure the system for collection of the P300 evoked potential Step 1 Start ACQUIRE as described above You will see the ACQUIRE main screen ACQUIRE4 2 20 Copyright 2001 Neurosoft Inc Document number 2202 Revision B v Gruss Zb ree ekeins te jon emaz aag For Heip mess Fi Pages E Retrieve the QCap32 ast file as described in the first tutorial We ll begin with it and make some modifica tions to it For your particular system you may want to use a different setup file if you have for example 64 channels or start with the Make Default Setup option as described in the first tutorial Step 2 The next step is to create a Setup
18. terminate acquisition when the terminate method 1s set to NOISE or BOTH on the designated channels Both Acquisition will be terminated if either the F or noise criteria are met Terminate Channel s The terminate channels fields allow you to specify Term Channels f Al C Selected which channels will be monitored for termination criteria All will terminate if any channel in the current montage reaches a criterion Selected will terminate on user selected channels Channels are selected using the ON OFF toggle box in the Individual Channel Settings under Edit As you step through the channels click on the Fsp Stop box to designate the selected channels a check mark will appear when selected Parameters The Parameters section lets you specify the following settings used in the Fsp analyses ACQUIRE4 2 117 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Parameters Single Point Pos ma SG Window Start ms CG Window Stop rns fio Sweeps per Block 250 F Value Di Notze Level p on Single Point Positive ms The point position value determines the location in milliseconds within the sample interval X min to X max of the single point estimate of the background noise Under normal circumstances this value would be placed within the bounds of the window start and stop point see below Window Start ms The Window Start point determines the starting loca
19. 1 0 0 0 0 0 0 0 0 0 0 0 16 20 00 OO OO 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 17 21 00 OO OO 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 20 24 00 OO OO 0 0 0 0 0 0 0 0 00 00 00 00 00 0 0 0 0 0 0 00 00 00 0 0 1 0 0 0 0 0 21 25 00 OO 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 00 0 0 0 0 0 0 0 0 0 0 00 0 0 0 0 1 0 0 0 2 6 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 000a 0a oo 0 0 0 0 J 7 OO 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 00 00 0 0 UU 6 10 00 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 00 00 00 00 0 0 0 0 11 O00 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 000a DUDU 0 0 0 0 10 14 00 0 0 OO 0 0 0 0 0 0 0 0 00 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 15 00 0 0 OO 0 0 0 0 0 0 0 0 0 0 0 0 00 1 0 0 0 0 0 0 0 1 0 00 00 0 0 0 0 0 0 0 0 0 0 Click Open and the file will appear in the window on the far right When you acquire the data you will see ACQUIRE4 2 56 Copyright 2001 Neurosoft Inc Document number 2202 Revision B the signals in a computed bipolar montage LDR operations are described more fully in the Operating EDIT section of the manual and in the Montage Editor Appendix at the end of the EDIT manual Select the polarity as desired Negative Up or Down Step 4 Now click the Amplifiers tab Set the A to D rate to 100Hz SynAmps users toggle to this value Next enter 32 f
20. 20 oe Duration ms 1400 EOG ACQUIRE4 2 130 Save calibration Values to Asch C Negative Threshold E T Mark acquired data files as read only fo Impedance operations Impedance lirit E EO hrs Mark high Hide high Inpedance impedance channels as bad channels Frompt after impedance check Load 3D positions The Load 30 positions button displays the standard Open File utility for selecting a 3DD file from 3DSpaceDx that contains the digitized electrode and PAN positions The 3DD file must agree with the electrode lables in the ACQUIRE setup file typically the ACQUIRE setup file will be used in 3DSpaceDx when the electrodes are digitized Be careful using this option The 3DD file that you load should be from the same subject whose EEG you are recording The 3DD file will become part of the setup file in ACQUIRE The position data will therefore be included with ANY subject s recordings obtained with the setup file which will of course result in incorrect position data for those subjects The option is intended for occasions where you are recording several EEG files from the SAME subject having a single 3DD file It is also possible to load the 3DD position data in EDIT as well as in SOURCE if you are unsure whether you want to add the file in ACQUIRE Copyright 2001 Neurosoft Inc Document number 2202 Revision B SE S librati Save calibration values to ASCII Clicking this button aa E opens
21. 2202 Revision B Disable the Baseline Correction option by clicking the Enable field so that the check mark does not appear ifneeded For later reference you might wish to enter the Start 200 and End Oms values To do this you should first Enable the feature enter the values then disable it the values will still be displayed Disable the Artifact Rejection feature by clicking on the Enable field so that the check mark does not appear if needed For later reference you might wish to enter the Start 200 and End 1500ms values and the Maximum 50uV and Minimum 50uV voltage thresholds To do this you should first Enable the feature enter the values then disable it the values will still be displayed Step amp For this recording we will also look at the online averages created for the combined non target words in the sentences the words leading up to the target word at the end of the sentence ordinarily these might be analyzed separately but for simplicity s sake they will be combined for this demonstration as well as for the congruent words and noncongruent words Assume that the STIM system has been set to send trigger type codes of 200 for the CONGRUENT stimuli and trigger type codes of 100 for the INCON GRUENT stimuli Assume also that the NON TARGET words leading up to the final target word have trigger type codes of 10 20 30 40 and 50 Click on the Sorting tab displayed in the Setup window We will first specify
22. Clicking this field allows you to specify the beginning of the recording epoch in milliseconds X minimum with respect to the trigger This value must be a negative number or zero For example if you want to begin the recording epoch 100ms prior to the stimulus enter minus 100 100 End ms Clicking this field allows you to specify the end of the recording epoch in milliseconds X maximum that is the end of the sampling epoch with respect to the trigger For example ifa 1000ms post trigger interval is desired set the end value to 1000 All sample intervals must include time ACQUIRE4 2 112 Copyright 2001 Neurosoft Inc Document number 2202 Revision B zero within the interval Itis not possible to construct an interval in which the X minimum is greater than zero or the X maximum is less than zero Note For continuous recordings the X minimum and X maximum values have no direct effect on the initial recording It is convenient however to set these values to an interval that will most likely be used when constructing offline epochs and averages with the EDIT module Points The number of points determines how many time samples will be made for each waveform at each electrode site The maximum number of points during acquisition is currently 65 536 divided by the number of current channels For example if you are recording from 32 channels you will be able to allocate up to 1024 points per channel Users of multiple S
23. Display field for Single Window Display No 2 This will activate the Set Filter Display button at the bottom of the Single Window Display No 1 awe M Enable FilterDisplay Derivation Derivation SR Single Window Display Mo 2 AA M Enable M Filter Display Derivation Denvation File Multiple Window Display SCC a H S z d S a S vere IT Enable Fiter Display Derivation Uem SAE Sorted Average Window Display Spectral Display Au W Enable d T Enable Set Display Fiter Negative C Up T Display this Menu on Startup Auto Save Window Click on this and you will see the Filter window In the Type region click the Band Pass button This will allow you to set the high and low pass filter settings On the Mode line select Analog Simulation if needed and leave the Rectify option Off In the Band Pass area enter 1 Hz for the high pass filter and select 24dB then enter 30 Hz for the Low pass filter setting with 24dB The effect of the filter setttings can be seen in the diagram window at the top of the display As with similar displays you may select the channels to be affected For this example we ll select all the channels Click the Select Al button d needed labels should be green Then click the S et Ge n button These settings will perform band pass digital filtering of frequencies outside the 1 30Hz range on all channels on the second Single Window Display Note Unlike the other similarly
24. M Freq 7 812 11 71 Deriv Original Map kee raw zl C M Mapa Beat o bn m rejin 1953 Gel Original well Eer S r PERIL IT Map 5 ES jo po RI Eeg WEE 29 06 Meny Original DES Ras gt Mone enter 0 and 30 These set the microvolt scaling limits for the online maps Under Domain click the Freq field so the check mark appears The Start and Stop frequencies for both delta and alpha maps have been set the exact values are determined by the AD rate We will not use the linear derivation mapping files at this point For Map Files use 3DSpace to create an anterior perspective 2D map for viewing delta and a posterior perspective 2D map for viewing alpha shown as 32chan in the above figure or use map files created in MAPGEN Click on the Map button and select the files with the Windows Open File utility map extension Under Data Source leave the Raw file selected Leave the Video buttons unselected these fields will be present only if you have a video camera attached Step 10 It would be a good idea at this point to save the settings that you have made thus far Click on Save As and label the Setup file CONTSET the ast extension will automatically be added and click Save Then click on OK Step 11 Click on Edit again at the Main Menu bar and click on Channel Layout You may resize and reposition not only the 32 channel array but also the 24 channel derived bipolar array Click the pull down arrow on the
25. Start Acquisition or just click on the green triangle toward the left end of the Toolbar P You will see the Multiple Windows Display and after initiating data storage two additional Multiple Windows Displays for the RARE and FREQ averaged files Start STIM or other acquisition system Note that the sorted averaged displays will have no data until the first trigger 1s received or first averaged update When you are ready to begin recording click on the Record icon the red dot on the Toolbar P I andentera file name The eeg extension will be added automatically Click on Save and you will be returned to the Multiple Window Displays The button to the right of the Record button with two vertical lines is the Pause button and the button with the black rectangle is the Stop button Shown below are the waveforms for the online RARE and FREQ sorted averages In this example we used a single window display rather than a Multiple Window Display to monitor the ongoing EEG ACQUIRE4 2 27 Copyright 2001 Neurosoft Inc Document number 2202 Revision B T I Fra Hep pee TI Page ri Ee el thee re Mmi age oe Status boxes will show the percentage of buffer space that is being used the amount of free space on your hard drive the number of accepted sweeps as well as additional information Refer to the View section in the Operating ACQUIRE details below for description of the Toolbar options for controlling acquis
26. The feature 1s more easily accessed from the Toolbar icon Calibration The calibration feature of ACQUIRE can be used to accomplish two things 1 Scale potential values to microvolt units and 2 Make software adjustments across channels to compensate for small differences in amplifier gains The Calibration option is selected from the Acquisition drop down menu or from the fay icon on the Toolbar Calibration signals may be generated internally or through the use of external signals For exter nal calibration signals it is assumed that you are able to generate a calibration pulse or continuous signal with a known peak to peak or baseline to peak value in the microvolt range Unless you have a special calibration signal device perhaps built into your amplifiers you will usually create the calibration signal by first generating the waveform in the range of volts and then attenuating the ACQUIRE4 2 158 Copyright 2001 Neurosoft Inc Document number 2202 Revision B signal to the microvolt range For example you may generate a 1 0 Volt pulse analog signal then use a 20 000 1 attenuator to reduce the 1 0 Volt signal to 50 microvolts Alternatively you might use a function generator in combination with an oscilloscope to generate and measure a continuous signal e g a 10Hz sine wave prior to attenuation Ordinarily all channels should receive a copy of the same calibration signal so that they are consistently calibrated and
27. The next step is to create a Setup file for continuous recordings For this example use the QCap32 ast file You can use a different setup file or the Mak Default Setup option as described in the first tutorial Step 3 Click Edit from the Main menu bar then click Overall Parameters Edit View Acquisition Options Overall Parameters Individual Channel Settings Channel Assignment Table Make default setup Subject Sunamps Hardware A multi card window will appear with the Startup card on top For this recording click on the fields to Enable the Single Display Window No and the Spectral Display For amore advanced demonstration let s say that you would also like to monitor the incoming data using a bipolar montage in addition to the unipolar linked ears montage that you are using for the recordings ACQUIRE4 2 55 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Single Window Display No 1 hg Enable FilterDisplay Derivation Derivation File Single Window Display No 2 AA W Enable Filter Display M Derivation Dernvation File 1020bip Multiple Window Display Wf Enable Filter Display Derivation Denvation File Sorted Average Window Display Spectral Display W Enable Set Weoley elter An IT Enable C Up T Display this Menu on Startup Auto Save You can do this by enabling the Single Window Display No 2 and clicking on the Derivation field Then cl
28. This 1s just a reminder 1t can be moved out of the way or closed or select the Do not show this dialog again option Impedance Electrode impedance may be measured via the Impedance command under the Acquisition menu as the electrodes are applied or between recordings to verify acceptable resistance levels Clicking on this option or going directly from the Toolbar icon Z displays the Impedance screen which Impedance O x 50 k hms 47 44 40 Wi a4 EH e Enter Range D eee Gem gg Maximum kOhms 50 Lancel 5 kOhme B32 a DIER consists of numbered or labeled color boxes corresponding to each channel Also displayed 1s a color scale calibrated in kiloOhms Note that the range of the scale may be varied by clicking on Range and entering the Threshold and Maximum kOhm values Typically the impedance screen is ACQUIRE4 2 157 Copyright 2001 Neurosoft Inc Document number 2202 Revision B displayed throughout the electrode application initially with a wide range on the color scale e g Maximum value of 50kOhms As the impedances drop down you might wish to use a more sensitive scale e g 0 to 10 kOhms to verify that all impedances are uniformly low e g at or below 5kOhms Setting the Minimum value to 5 kOhms will result in the appearance of a black square when the impedance drops below 5 kOhms You might wish to check the impedances periodically betwe
29. Update Dn Display Start rng j 0 Stop ms 100 Artifact Rejection W Enable Start ma End ms D DN Y Min Mm p Lon Wan Jul 50 Y Max IT Y Autoscale Baseline Correction W Enable Start ms 0 End me E this example assume that you want a 10ms pre stimulus interval a 100ms post stimulus span and the artifact rejection and automatic baseline correction features enabled during acquisition In the area labeled General set the number of Sweeps to 1000 Set the Start time for 10 pre stimulus and the end time for 100ms The sample interval has now been set with a pre stimulus interval of 10ms and a post stimulus interval of 100ms Click on the Points field and enter 1101 if needed for non SynAmps systems enter 550 The Avg Update field is not accessible for this operation In this example a total sample interval of 111ms 10ms pre stimulus 100ms post stimulus and the zero point has been selected The stimulation protocol must therefore allow for at least this interval plus addi ACQUIRE4 2 34 Copyright 2001 Neurosoft Inc Document number 2202 Revision B tional time for the processing overhead of the computer A stimulation rate of about 5 6 per second is recommended in this example If faster stimulation rates are desired you can decrease the sample interval as long as the post stimulus interval is of sufficient duration to view the major response components The pro
30. Window Settings Clicking on the Multiple Windows Settings option displays a dialog box containing 3 option tabs the X Axis the Y Axis and General X Axis The X Axis page is divided into 3 regions Single X Axis Tick marks and Scaling o DN Y Axis General Single a Asis Tick marks Enable W Label Ge Baseline Step size Time C Bottom Auto Step size Freg Auto T W Time zero Single X Axis Click on the Enable field check mark will appear to display the x axis in the multiple windows displays for averaged or epoched files Next select whether you would like the axis to appear at the top the baseline or bottom of the waveform display Click on the Time Zero box to have a vertical line drawn at time zero Tick marks Click on the Enable field if you would like tick marks displayed on the x axis Click on the Label button if you would like labels to appear under the tick marks Under the Step size options Time and Freq you may select the interval between successive tick marks The Time domain steps are in ms and the Frequency domain steps are in Hz Click on the pull down arrow indicator and highlight click the desired option range 1s from 0 1 ms to 100 seconds under the Time field and 5 Hz to 10 kHz under the Freq field or select Auto to let the program place the marks automatically When you are satisfied with the settings click on the Save As button to add the inform
31. a horizontal manner stretching from one side of the screen to the other top to bottom With multiple windows e g more than 3 however the Tile Horizontal or Tile Vertical option will arrange the window displays in an well organized fashion as shown below Tile Vertically Tile Vertically will automatically arrange windows in a vertical manner stretching from the top of the screen to the bottom side by side With multiple windows however the Tile Vertical or Tile Horizontal option will arrange the window displays in an well organized fashion as shown above ACQUIRE4 2 176 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Acquire 64 channels File Edt View Acquistion Options Window Help 1 Help The Help option accesses the ACQUIRE Manual Internet Help and About ACQUIRE lines Help Acquire Manual Batch Manual Source Manual Internet Help About ACQUIRE Reset Workspace ACQUIRE Manual This selection will give you immediate access to this manual in PDF format BATCH Manual This selection will give you immediate access to the Tcl Batch Commands manual in PDF format SOURCE Manual This selection will give you immediate access to the SOURCE manual in PDF format Internet Help This section will connect to our web site www neuro com for Scan 4 2 From there you may go to the Technical Notes or other sections for additional information or files to download About ACQUIR
32. a white background behind the electrode label and Selected Channel the label will be displayed in the Fz e field You can use the up and down arrow buttons to select a individual electrode or click the mouse button once on an electrode label When you have selected the channels that you wish to modify enter the desired Gain Low Pass and High Pass filter settings in the Amplifier Settings fields Then click the e aan n button to apply the changes You may verify the changes you have made by clicking an electrode label once and looking at the values in the Amplifier Settings display Click the ACQUIRE4 2 103 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 104 Copyright 2001 Neurosoft Inc Document number 2202 Revision B OK button to return to the main screen in ACQUIRE or click Cancel to leave the screen without applying the changes you have made Gain The gain is used to calibrate the incoming signals from volt to micro volt units This gain factor affects all channels SynAmps users may toggle from among settings specifically calculated for their systems NuAmps have a single fixed Gain of 19 Non SynAmps users may enter integers directly from the keyboard although once set in the A D card installation these should not be changed unless you physically change the gain on the amplifi ers or otherwise change the attenuation With SynAmps you may use amplifiers with differe
33. add new keys place the cursor in the Hot Key field and press the key Enter the text comments and click Add you will get an error message if the key is already being used for another purpose Another way 1s to insert strings of text On the left hand side of the Window under Annota tions enter a text string and click the Add button When you click the Annotation icon from the Toolbar described below you can select the line that you wish to add Ifyou want to remove a line from the Annotations list highlight it and click Remove ACQUIRE4 2 129 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Lastly you can insert text on the fly during acquisition by clicking the Annotation icon and then entering any text you wish The comment will appear at the point where you clicked the icon you must be saving the data for the Annotation icon to be active Mise Several additional options are accessed from the Misc screen Ocular artitact reduction Ocular artifact reduction These fields allow you to enter the ocular artifact reduction parameters that will be used when you apply the transform in EDIT The parameters you set inan ACQUIRE setup file will be saved with data files and will be present in EDIT so you will not have to enter them in EDIT See the Ocular Artifact Reduction description in the EDIT manual for details about the parameters Trigger Load 3D positions Elink values Min sweeps
34. cap cable problems You should resolve these problems before acquiring the data Toolbar Selecting this feature activates the display of the Tool bar near the top of the screen This is a very handy feature since it allows rapid selection of options available during acquisition The options w Pe nm tt Ti Bt en e oe LZ ajA Peep are highlighted when they are accessible This depends upon where you are in the Acquisition process These features are explained briefly below and more thoroughly above Select Setup File Clicking this option opens a standard Open File utility for selecting Scan 4 0 or Scan 4 2 setup files ast extensions Recording Controls P il These four buttons function similarly to those ona tape recorder The green arrow indicator fP will either start the acquisition in a monitor mode not saving the data or bring up the Start Acquisition dialog box depending on how it was set on the Startup screen under Edit Overall Parameters The red button 1s the Record button Clicking it opens a Windows Save File utility for allowing you to enter a file name and designate the directory in which you want the data to be stored Data storage will begin after you click the Save button on the Save As display If during a recording you click the Record button a second time data storage will be suspended clicking it again will continue the save to the same file The button with the two vertical line
35. file and enter all of the informa tion for it Repeat this for each sorted file you wish to create Lastly click on the Export button in the Sort file template area and enter a file name for the sorting file the s7t extension will be added automatically Click on the individual average files under the ACQUIRE4 2 121 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Enable Online Averaging line to review the settings for each file Notice that in this process you can for example have two averages that are exactly the same except that one could have the Artifact Rejection option enabled and the other average could include all sweeps You can in this way see the effects of the various parameter settings on the same data file Criteria You may sort on the basis of stimulus and response criteria You may define these independently or in combination Stimulus You may sort on the basis of the Stimulus type codes Ina typical auditory P300 paradigm let s say the FREQ tones all had type codes of 100 and the RARE tones had type codes of 200 One average would be for the FREQ stimuli coded as 100 in the Stimulus field and the other average would be for the RARE stimuli coded as 200 in the Stimulus field The convention for all criteria fields 1s as follows For non sequential numbers place a comma between the numbers such as 1 5 9 For sequen tial numbers place a hyphen between the first and last number in the ran
36. file created in MapGen that has the same number of electrodes and labels as the derived bipolar montage refer to the MapGen manual if you are not familiar with creating map files The following figure shows the relevant features of the setup file in ACQUIRE The Startup page under Overall Parameters under Edit should have one Single Window Display in which the bipolar dr file is loaded in this case the file was called 1020BIP the other Single Window Display No 1 awe MW Enable FilterDisplay M Derivation Derivation File 1020bip Single Window Display Mo 2 AA W Enable FilterDisplay Derivation Cenvation File Multiple Window Display e MW Enable FilterDisplay M Derivation Denvation File region Sorted Average Window Display Spectral Display An Enable d M Enable Display this Menu on Startup Auto Save Set Wola Eter Single Window Display set for raw data acquisition and the Multiple Window Display set for the composite waveform dr file in this case the file was called REGION Notice that the Spectral Display 1s activated This occurs automatically when you enable online mapping You will need to enable the online mapping option and designate the bipolar dr file as well as its corresponding map file ACQUIRE4 2 85 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Label Min Max Domain Start Stop LDR File Name Map File Name Data Source M Map
37. file in 4 2 and it will show 50 to 499ms The last point is dropped auto matically there is no latency shift When you retrieve your prior setup files in AC QUIRE 4 2 the epoch settings will be automatically adjusted to insure that old and new files will be compatible The only way to encounter problems is if you for ex ample create a new setup file where you specify 50 to 500ms and try to compare those files with older ones The new ones will have one additional data point so the files cannot be compared Should that happen the Cut Epoch transform can be used to remove the additional point from the end of the file General These parameters control the number of sweeps to be acquired the starting and ending time points of the recording epoch the number of points per epoch and the average update setting Sweeps Clicking this field allows you to input the number of sweeps you wish to acquire Ifyou anticipate acquiring an undetermined or large number of sweeps then put a value that you will likely not exceed in this field maximum value is 65 535 Data will be saved to the disk on a sweep by sweep basis until the sweep number 1s reached or until you terminate acquisition by pressing the STOP key This parameter has no effect for continuous recordings Note 1f you are saving single sweeps and you have specified more sweeps than the current disk has space for you will receive a warning that you will run out of space Start ms
38. gt gt bar An extension to the color palette will appear In the large color screen catch the reticule with the mouse move it around the spectrum and see the corresponding color in the Color Solid box below Notice also the intensity bar on the far right Catch the triangular indicator with the mouse and slide it up and down to vary the intensity of the color in the Color Solid box When you have decided on a color click on the Add to Custom Colors bar and the color will be repeated in one of the Custom boxes on the original Color screen Create as many customized Copyright 2001 Neurosoft Inc Document number 2202 Revision B Color Basic colors Custom colors ll SHH ll 1 EE ll SEED ll RS EI EE EI Hue eu Red 174 Sat 140 JE Green 225 beine Eusam Eolas gt Colors alid Lum fi 72 Blue li A Cancel Add ta Custom Colors colors as you wish Notice that each of the display features options will bring up the color palette with the custom colors you have created To select one of the custom colors click on the box then click OK to invoke the color for the display feature you had selected The Frequency Bands option is the only exception to the other Colors options Clicking on this option displays the Frequency Band Colors window With this feature you may 1 label up to eight EEG frequency bands 2 define the start and stop frequency limits for up to eight EEG frequency bands and 3 sele
39. has the focus for the new frequency range When you are ready to begin recording click on the Record icon the red dot on the Toolbar Feu e and a Windows Save File utility will appear Enter a file name for the continuous file and any path changes the cnt extension will be added automatically and data will begin to be written to the disk when you click OK The recording may be terminated by clicking on the Stop icon on the tool bar Refer to the View section in the Operating ACQUIRE details below for description of the Toolbar options for controlling acquisition Note When you close ACQUIRE after performing any operation that modifies the setup file you will see a message asking if you want to save the modifies setup file ACQUIRE4 2 63 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Ki Setup has been modified Save How Click Yes if you want to save the modified setup file and No if you want to exit without saving the file This concludes the Tutorial sections ACQUIRE4 2 64 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Operating ACQUIRE The remainder of this manual details the operations found in ACQUIRE For the most part the features are organized ma left to right and top to bottom fashion That is explanations begin with the Main Menu bar options on the left and proceed across the menu bar The features under each are explained from the top one to the
40. have made thus far Click on File Edt View 4c Save As Load setup and label the Setup file N400SET the det extension will automatically be Save Setup added and click Save Then click on OK If you want to resize or reposition the electrode displays go to the Channel Layout option under Edit as described in some of the previous tutorials Step 11 To begin viewing click Acquisition on the Main menu bar followed by Start Acquisition or just click on the green triangle toward the left end of the Toolbar gt You will see the 2 Single Window Displays and the 3 sorted average window displays The effects of the online filtering should be apparent in the second Single Window Display When you are ready to begin recording click on the Record icon the red dot onthe Toolbar P IS cally Click on Save and you will be returned to the Single and Averaged Window Displays Note that ACQUIRE4 2 53 Copyright 2001 Neurosoft Inc Document number 2202 Revision B and enter a file name The cnt extension will be added automati only the Single Window Display No I will be saved the second Single Window Display is for display purposes only In the Averaged windows you will see the average wave forms develop with updates as specified in the Epoch section under Edit Overall Parameters Sorting Refer to the description of the Options section below in the Operating ACQUIRE part of the manual to see ho
41. in balance A diagram of a 20000 1 attenuator is shown below Input 1 Vp p Output SOu If you choose to use the internal SCAN analog output to generate the calibration pulse as with the Metrabyte card or LabMaster card you will only need to supply an attenuator device This device should 1 convert a signal from the range of volts to microvolts and 2 send the calibration pulse to all amplifier or headbox channels Typically the latter is achieved by inputting the signal into 2 jacks on the headbox and then selecting these on the electrode selector panel for all channels Internal Calibration LabMaster and Metrabyte systems Internal calibration makes use ofa known calibration signal originating from either the LabMaster or Metrabyte cards With the LabMaster card use the Analog output jack on the back of the SCAN Acquisition box headphone jack and with the Metrabyte card use the mini phone jack labeled Cal on the pigtail box on the cable from the amplifiers to the SCAN PC Note these are the most common LabMaster and Metrabyte configurations your specific system could differ somewhat The output from either card is typically a 1V 10Hz sine wave You will need to provide an attenuator that reduces the voltage to 5OuVs as described above The following equation describes the variables and operations involved AD output voltage attenuator X amplification amplifier output where the AD output voltage is the voltag
42. online average This toggle has no effect on the single sweep EEG file nor does it affect offline baseline correction in the EDIT module ACQUIRE4 2 113 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Start ms The Start point sets the beginning of the interval in which the mean D C offset is estimated Stop ms The Stop point sets the end of the interval in which the mean D C offset is estimated Here are a couple of examples that illustrate the use of baseline correction Correcting the baseline using the entire interval Suppose you are recording spontaneous EEG with a sample period of 2 56 seconds or 2560ms To compute a baseline adjustment correction factor for the entire sample epoch enter 0 for the start point and 2560 for the end point These values will result in the entire sample epoch centered on the baseline witha mean of zero Correcting the baseline using the prestimulus interval Suppose you are recording an evoked potential with a pre stimulus sample period of 200ms and a post stimulus period of 800ms To compute a baseline adjustment based on the pre stimulus period enter 200 200ms prior to stimulus onset for the start point and 0 Oms prior to stimulus onset for the end point These values will result in an averaged evoked potential that is baseline corrected in the pre stimulus sampling period Artifact Rejection These parameters control the automatic rejection of sweeps during acqu
43. program close due to some illegal operation and you still see the arrow on the icon it is necessary to close and reopen the Program Launcher before reopening ACQUIRE To complete the installation you will need to enter settings specific for your system After starting AC QUIRE click on Edit At the bottom of the pull down menu you should see the option specific for your Edit View Acquisition Options Overall Parameters Channel Layout Channel 4ssignment Table Make default setup Subject Spnamps Hardware system as described above 1 e SynAmps Hardware etc ACQUIRE4 2 66 Copyright 2001 Neurosoft Inc Document number 2202 Revision B SynAmps If you have a SynAmps system select the SynAmps Hardware option and a dialog box will appear Indicate the appropriate settings for your system Simp ETC ETC GIE d Mea Her Usage C DC Head D ACDC He riets E Geen Test Triope Post Cunard Dire amp Alo f1 EC yaiz aiai 0 EC Des 23a EC Tae 72 47 21 DAF Daa 2138 OSP Tee 525m AC DC Indicate whether the SynAmps are DC only or AC DC newer systems are AC DC Head Gain Select either 30 or 150x gain newer systems are 150x Current Drivers The Current Drivers fields show the EC exe file version date and time and the DSPSYNAM exe date and time for the SynAmps indicated in the Amp No field Click on the down arrow beside this field to see additional SynAmps If these fiel
44. resize the displays and import export electrode position files Size and Position 10 20 Positions Reset Positions Make Same Size Import Positions Export Positions Adjust Positions 10 20 Positions This button is used to position the electrode displays automati cally according to the 10 20 system or from electrode position information con tained in the 3DD file created in 3DSpaceDx when the electrode positions were digitized ACQUIRE4 2 135 Copyright 2001 Neurosoft Inc Document number 2202 Revision B 10 20 Electrode Positions 106 76 00 18 l 135 27 142 70 Ep FEZ ni 176 33 e 7 ES 169 45 137 55 125 30 105 87 Bd 24 S000 6 61 138 06 94 rads 53 96 41 75 17 53 4 50 33 64 41 72 30 00 69 73 17 39 160 18 40 24 ere Chae Match Labels Cartesian to Polar Load 3D pos Polar to Cartesian SS ie oe Snapto Grid Theta Di D in Current Pos Phi 1 5 Th 115 3 o K Cancel ACQUIRE4 2 136 Two electrode positioning systems are supported Cartesian and Polar The Cartesian positioning system is the X Y and Z coordinate system where the x axis runs from the left ear negative to the right ear positive the y axis runs from the nasion positive through the inion negative and the z axis runs from the vertex positive though the intersection of the x and y axes to points below negative The 0 0 0 point
45. respectively These settings affect the display of the averaged waveforms the entire epoch will be displayed and the amplitude will be scaled to 100uVs Do not click on the Y Autoscale for these recordings Step amp To perform the online average let s assume that the SEP stimulator is being controlled by the STIM system and that STIM is sending a trigger type code of 1 via the STIM to SCAN cable to the SCAN system While we are not actually sorting the stimuli we can use the Sorting option to create an M Enable Online Averaging Criteria cc Add Average MW Stimulus fi 0 IT Response p gt gt Del Average gt gt Del Average Sweep Start mz stop ms Points Parameters Avg Update li Baseline Correction W Enable Start riz UU Stop ms 0 Sort file Export Import Display P Y Autoscale Start m 100 Stop ma 100 0 Y Min Wil Jonn YMan uv 100 0 Artifact Rejection T Enable Stant mel bn stomme bmn Hing ao Flere ui bmn Foo oer DER ACQUIRE4 2 35 Copyright 2001 Neurosoft Inc Document number 2202 Revision B online SEP average Click on the Sorting tab and click the Enable field on if needed check mark will appear Click on the lt lt Add Average button and a Windows Save file utility will appear Enter a file name such as SEP the avg extension is added automatically and click Save Next en
46. set it to be an Artifact Rejection channel If you have Artifact Rejection displayed in the Multi Channel Set field the Atrifact Rejection will be applied automatically when you select the channel You can resize and reposition the display shortly if desired Leave the Fy stop field empty This field allows you to designate channels as termination channels for use with Fp averaging discussed below Since we are only using the one channel and will be selecting All for the Termination channels on the F page it is not necessary to designate the channel with the Fs option on the Channel Setup window If we were recording from several channels and had chosen Selected under Termination channels on the Fsp page we could designate which channels were to be used as termination channels by clicking on the F field in the same fashion as we designate artifact reyection channels ACQUIRE4 2 41 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Properties Multi Channel Set Artifact Rejection Selected Channel EI cz iS Physical Channel r Calibration Impedance fff he Artifact Rejection T Fesp stop T Hide Skip Operations T Auto Add Last IT Bad IT Auto Add Default a Units li 00 Add After Defaut Y Units Colors Single Mut Ce Default Ge Default C Custom Custom Waveboard Deselect Al Step 6 Triggering for the ABR will be controlled by an externa
47. setting configures ACQUIRE to store the single sweep data If you are using a SynAmps under AC DC select AC mode If you are not using SynAmps this section will not be acces sible Under Amplifier Settings set the gain to 1000x the Low Pass filter to 1000Hz and the High Pass filter to 1Hz SynAmps only Leave the Notch Filter OFF no check mark in the box Click the Select All button all channels should be green and then click the g E E button to apply the gain and filter settings to all channels For non SynAmps systems these values are set in the Metrabyte or LabMaster option under Edit from the Main menu bar These settings should not be changed unless you physically reset the amplifiers re enter the values on the Hardware screen under Edit and recalibrate with those settings ACQUIRE4 2 30 Copyright 2001 Neurosoft Inc Document number 2202 Revision B 10000 z Epoched E ba Ee Tooo He z Tone z Step 5 Now click the Channel Attributes tab Since we started with the Make Default option the chan nels appear with numbers instead of labels To change these to labels go to the Selected Channel region and change the 1 to C3 EI then click the SelectAll button to transfer the new label to the display on the right side Then click the Up Arrow button to go to the next channel Change it to C3 Continue to process until you have renamed the channels to C3 C3 C4 and CA We will reposition the displays
48. setup file Step 13 To begin recording click Acquisition on the Main menu bar followed by Start Acquisition or just click on the green triangle toward the left end of the Toolbar You will see the Multiple Window ACQUIRE4 2 37 Copyright 2001 Neurosoft Inc Document number 2202 Revision B re i Hi z Lees ker Ti ed A Cie Ern yes ZGeorpem pee Gre fee e EF eum A For Hina Ha FI Peet BAIN nn Display and after initiating data storage the sorted Average window display When you are ready to begin recording click on the Record icon the red dot on the Toolbar P Il andentera file name The eeg extension will be added automatically Click on Save and you will be returned to the Multiple Window Displays The adjacent button with two vertical lines is the Pause button and the button with the black rectangle is the Stop button In the averaged window display you will see the average waveform develop with updates as specified in the Epoch section under Edit Overall Parameters Note that you will not see any data in the average display until the first trigger is received or until you have reached the average update number of sweeps Refer to the description of the Options section below in the Operating ACQUIRE part of the manual to see how to change display colors and to select different aspects of the window display A Status box will show the percentage of buffe
49. setup file to the second one Adjust Positions The Adjust Positions option is used to reposition or resize the electrode displays automatically Clicking the button displays the following screen Electrode Position Scaling K The three groups of adjustments are for Spacing Size and Translation Spacing expands or compresses the grouping of the displays either vertically or horizon tally lr Similarly the Size buttons increase or decrease the size of the elec trode displays either vertically or horizontally The Translation buttons will shift the entire grouping of the displays vertically or horizontally Channel Assignment Table The Channel Assignment Table displays the Channel Assignment screen consisting of the first 32 physical channels and their corresponding labels SCAN can be configured to operate with from 1 to 64 channels and ESI can be configured from 1 to 256 channels with SynAmps in groups of 32 channels Additional channel assignment screens will be accessible 1f more than 32 channels are selected in the Edit Overall Parameters Amplifiers display Electrode labels may be substituted for the default numbers by clicking on the label fields and inputting the desired electrode label from the keyboard Electrode channel assignment can be remapped with the LabMaster Metrabyte and SynAmps systems ACQUIRE4 2 140 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Phys Chn Label
50. site when you position the mouse over the elec trode Use Linear Method The default interpolation method is the Global Method which uses all sites in the interpolation The Linear Method typically uses the 4 nearest electrodes for interpolation The former pro duces a smoother map but may take slightly longer to compute Computa tional details may be found in the EDIT manual Events These fields allow you to enter various comments into the continuous file during acquisition These comments may be viewed when you Replay the data file in EDIT Annotations Enter new annotatior BLN Blink LOU Cough DRAM Drowsiness ECG Electrocardiograrn F5 Enter F5 text Eves closed Hyperventilation start Hyperventilation stop Movement Heart beat EMG Artifact EOG Electrooculogram MOM Movement MSC Muscle SLP Sleep SWL Swallow De Seizure Add Add Remove There are several ways you can insert comments in the continuous file The numerical keys from 1 through 7 have been set with the comments seen in the Events window These can be changed or used as they are During acquisition just press the key for the comment to be inserted To change one of these default comments highlight the key you want to change such as 7 and EMG artifact then click the Remove button Place the cursor in the Hot Key field and press the 7 key Then enter whatever comment you wish in the Text field and press Add To
51. superimposed channels will appear in that display To disable the Compare option delete all electrodes from the Comparison List Channel Properties Clicking this option displays the Electrode Properties screen From this screen you may designate a channel on the fly as an Artifact Rejection Bad Skip and so forth channel These are the same options that you see in the Overall Parameters Channel Attributes screen ACQUIRE4 2 92 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Electrode properties Curent Electrode C3 Artifact Rejection T Fep stop Hide Skip Operations Bad T Auto Add 20 Add After T Auto Add Last SNA Calib Scalar OF Default as Uniks Defaut Units Cancel UAT Butterfly Plot Selecting this option will superimpose all channels together plus calculate the Mean Global Field Power for the displayed epoch The data will be redisplayed as shown below 400 0 300 0 200 0 100 0 ari a ee A KP Dese EE vg 100 0 aia A Pee P SW V A z EE VI 200 0 300 0 400 0 500 0 400 0 300 0 200 0 100 0 UU 100 0 200 0 3000 4000 ms hi FP 150 0 125 0 100 0 2 750 50 0 25 0 0 0 500 0 400 0 2000 200 0 100 0 OU 100 0 400 0 3000 400 0 ms ACQUIRE4 2 93 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Deselect the option or just close the window to return to the original display Sor
52. sweep see Operating ACQUIRE below for more details Refer to the View section in the Operating ACQUIRE details below for description of the Toolbar options for controlling acquisition Termination will occur when either termination criterion 1s met the 20 000 sweeps are accepted or you click on Stop on the Toolbar ACQUIRE4 2 46 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Continuous recording online EMG audio output and online filtering N400 The continuous direct to disk recording mode of ACQUIRE can be used when a gap free recording of EEG is required Data are stored along with event markers in a continuous format One of the primary advantages of a continuous recording is that it provides flexibility for offline data analyses The data can be replayed and processed any number of times to explore different hypotheses With a continuous recording it is a simple matter to change the size of an epoch New pre and post stimulus intervals can be recom puted for any length limited only by available memory with the EDIT module Data recorded in the epoched mode however remain fixed and cannot be transformed Another advantage to the continuous recording is that epochs can overlap This feature is particularly important in more complex paradigms in which multiple events may occur in close temporal proximity Cognitive tasks that explore attention and memory often have events that generate overlapping epochs A continuou
53. that you have your video camera correctly installed and running Clicking the option will display the camera view The complete functioning of the video capabilities is described above in the Toolbar section Acquire 64 channels File Edit View Acquisition Options Window Help 1 Acquisition Clicking on Acquisition on the Main menu bar activates a pull down menu containing a variety of options Different ones will be available depending upon where you are in the acquisition process Most of them may be accessed more easily from the Toolbar icons or from right mouse button menus Acquisition Options Window Help Start Acquistion Liv Impedance Lil mpedence ahhegatite Lil Calibration Shitt Ctrl C stop ill Restart Reto Lil koechielou EFE Annotator EES Ear eaveTage Send Waremme bon Wwe wena Start Acquisition When you click on Start Acquisition or click the green arrow on the left end of the Toolbar you will see either the Startup dialog box or the acquisition Display window s depending on whether you clicked the Display this menu on Startup field on the Startup page under Edit Overall Parameters Please refer to that section for a description of the features in the Startup window When the acquisition display appears you will see a message saying ACQUIRE4 2 156 Copyright 2001 Neurosoft Inc Document number 2202 Revision B roan A Data Not Recording Record button from Tool Bar to Start i gt
54. the standard Save As utility window Use it to select a path and to enter a file name DAT extension added automatically The calibration values will be stored in this file and may be reviewed with a text editor Mark acquired data files as read only When enabled this option will automati cally mark the acquired data files as Read Only files thus prevent any unintentional modifications in EDIT M Mark acquired data files as read only To unmark the data files locate the file using the Windows Explorer Right click on it and go to Properties De select Read Only in the Attributes section Impedance operations These options are used to set automatically channels as either Bad or Hide channels when the impedance level exceeds the threshold Impedance limit kKOhms value you enter in the Impedance limit field E This is particularly useful in 128 or 256 channel recordings where it is not unusual to have a few M ark high Hide High channels with high impedances The buttons mpedance impedance when channels as bad channels clicked will set the high impedance channels as Bad channels which will exclude them from many of the offline analyses in EDIT OR as Hide channels which will hide those channels from the display the data are still recorded You may select either option but not both The Prompt after impedance check Option when enabled will prompt you to use the options Note Be sure to SAVE your setup f
55. the Toolbar The default setting 1s negative voltage up Perform DC Correction SynAmps only Performs single DC correction with each use to remove DC components will center signals at the baseline DC corrections can be performed manually by clicking the icon see below or auto matically when you Enable the DC Correction option see AC DC section above When a DC correction occurs you will see a DCC event marker at the bottom of the Single Window display along with the stimulus and response events The DC corrections will be stored in the data file and are treated as events in the file ACQUIRE4 2 145 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Considerations about DC corrections Whena DC correction occurs it creates a discontinuity in the data immediately surrounding the correction For that reason the Epoch File transform in EDIT will not create an epoch if the epoch contains a DC correction The DC correction events are considered permanent for the same reason Therefore you should use DC corrections only sparingly If you have frequent DC corrections you may wind up with a data file that cannot be epoched If there are frequent automatic DC corrections or if you find that you have to apply frequent manual DC corrections those are indications of an acquisition problem with one or more channels There could be high impedance a bad electrode lead or wire a DC battery potential problem or other
56. the speed of the hardware and the number of operations that are performed online ACQUIRE4 2 99 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 100 In general these operations are performed between the sample interval or pauses between stimulus presentations Activities such as screen update and disk access are among the most time consumptive Other activities such as baseline correction artifact rejection and F averaging also require varying amounts of time During these activities your system is blind to trigger pulses that arrive at the trigger port Note SynAmps based systems are not affected in this manner Ifa trigger pulse arrives while the system is engaged in either of these activities it will miss a sweep To avoid this problem make sure that your interstimulus interval is sufficient to allow these activities to occur The length of this interval depends on How fast your system can write data to disk 2 How fast it can refresh the screen and 3 The computational speed of your processor The best way to ensure that you will not miss any sweeps is to perform a test run and make sure that your system can keep up with the parameters that you have specified Special configuration concerns for continuous acquisition Data throughput Continuous direct to disk recording is a difficult task for any computer to perform Special attention must be paid to the digitization rate and number of ch
57. they are green Make sure that Artifact Rejection is displayed in the Multi Channel Set field The Artifact Rejection field will be checked for these channels You will see an asterisk beside the elec trode label for these channels when the data files are displayed online or offline Double click channel to change the selected Properties Mult Channel Set Artifact Rejection Selected Channel EI WEOG ia Colors Single Milti Physical Channel maoo Ce Default Ce Default Calibration Impedance T Custom 7 Custom fi fo E enea Raeina T IT Fesp stop Hide Waveboard Skip Operations T Auto Add Last l Bad T Auto Add Default a Units la Add After Defaut Units Select All Deselect All ACQUIRE4 2 50 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Step 6 Triggering for the continuous recording will be controlled by an External device 1 e STIM Startup Amplifiers Channel Attributes Triggers Ge External Hold value lo IT Use inverted values E nemal literal e oz system The Hold value should be set to 0 see the triggering section in the Operating ACQUIRE section below for special circumstances The Voltage fields are not relevant for these recordings assume triggers are coming from the STIM system Step 7 Click on the Epochs label to set the parameters of the recording epoch The number of Sweeps in the General section is not relevant in continuous rec
58. this group of 8 electrodes will be used Virtual Ground This option should be used only for demonstration purposes while recording signals from the PocketTrace signal generator In that case the program displays pure potentials under active electrodes relative to ground Such traces however may have 50 60 Hz noise DC drift etc The Virtual Ground can be used only for high amplitude signals such as ECG For low amplitude signals like EEG differential montages are used and the reference potentials for example the earlobe are subtracted from the active potentials Bipolar recordings You may be wondering at this point how to set up a bipolar montage where there is no single reference Bipolar montages are computed from the EEG signals by simple subtraction The Montage Editor is used to create an LDR file linear derivation file that performs the subtraction and the LDR file may be applied online or offline Please see the NuAmps manual for step by step instructions and the Montage Editor appendix at the end of this manual for ACQUIRE4 2 71 Copyright 2001 Neurosoft Inc Document number 2202 Revision B operational details for it Trigger Test The Trigger Test display shows the state of the stimulus and response bits at the port on the NuAmps where the stimulus triggers connect Itis used diagnostically in cases where you are having difficulties seeing triggers The functioning is identical to that described for the SynAm
59. to modify and the other fields are used to select the attributes that are to be applied to the selected channels Properties Multi Channel Set Vulle Te el Selected Channel Jel VEOG fa Colors Single Mut Physical Channel 32 Calibration Impedance RR M Artifact Rejection IT Fesp stop T Hide Skip Operations TT Auto Add Last Bad T Auto Add Default a Units lo Add Alter Default Y Units C Default C Default Ce Custom fe Custom Waveboard Select Al Deselect All Selecting Channels Channels may be selected through several methods Click the electrode label once to select an individual channel The background area behind it will turn Selected Channel white rz and the electrode will appear in the Fz field You can also use the up and down arrow buttons to step through the electrodes until you reach a desired one You can change the selected or deselected status of an individual channel by double clicking the electrode label This is useful when you want to select multiple electrodes for the same modification When selected the electrode label will be green when deselected the label willbe red Use the SelectAll button to select all the channels and the Deselect All button to deselect all channels Double clicking an electrode label also applies to that channel the modification that 1s displayed in the Multi Channel Set field The opera tions may be
60. you to specify a number of param eters for each channel collectively or individually please refer to the Operating ACQUIRE section for complete details For this demonstration we will set the VEOG and HEOG eye artifact channels as the Artifact Rejection channels These channels will then be used by the automatic Artifact Rejection scan online or offline Detection of values in excess of the criteria set in the Artifact Rejection fields under Epochs described below will result in rejection of the sweep There are several ways through which you can set channels as Artifact Rejection channels The easiest way here is to double click the mouse on the ACQUIRE4 2 14 Copyright 2001 Neurosoft Inc Document number 2202 Revision B VEOG and HEOG labels on the electrode display so they are green and the rest of the channels are red Make sure the Artifact Rejection is displayed in the Multi Channel Set field When you double click one of Selected Channel the channels you will see that channel label appear in the Selected Channel field Freng H anda T check appear in the Artifact Rejection field kg Artifact Rejection The full display will appear similar to the following figure Properties Mul Channel Set Artifact Rejection Selected Channel Colors HEOG E Single Mutt Physical Channel br Ge Defaut Ge Default Calibration Impedance C Custom C Custom f ja hg Artifact Rejection eee IT Fsp stop Hi
61. you will be using with your amplifiers frequencies above this value will be attenuated This value should correspond to the actual setting on the amplifiers High pass Hz Enter your high pass filter setting that you will be using with your amplifiers frequencies below this value will be attenuated This value should correspond to the actual setting on the amplifiers Note Users frequently acquire data with a very wide range in filter settings e g 0 1Hz high pass and 100Hz low pass Additional filtering and refiltering can be performed offline without affecting the original data Trigger Out Voltage Default resting V The Trigger Out is available for the control of external devices Typically the resting voltage 1s 0 0uV but you may set it to any value up to 5 0V Active V The active state of the Trigger Out may be set to any voltage up to 5 0V depending on the requirements of the external device ACQUIRE4 2 73 Copyright 2001 Neurosoft Inc Document number 2202 Revision B A D Converter Range The A D converter has a default setting of 1 0uV but may be set to receive signals in the 5uV range to provide better resolution of relatively low amplitude signals T O Input Output Base address hex Select from among the choices the correct base I O address hex value Typically this will be 340 unless there are conflicts with other base I O addresses Other settings will require corresponding changes on the
62. you wish to show the Seconds Lines and the 200ms Lines in the display as well as a lower resolution option ACQUIRE4 2 173 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Single Window Options Wired M Display Seconds Line i Display 200me Line Windom 2 M Display Seconds Line h Display 200mes Line Faster lower resolution f U Seconds per page Waveform color WW Background color Cursor color Scale Box C Opaque T Faster lower resolution fio Seconds per page Waveform color E Background color Cursor color Scale Bow C Opaque Ge Transparent Ge Transparent check mark will appear when enabled Faster lower resolution displays are useful with the higher sampling rates as with EMG recordings in which there may actually be more points than pixels You may also select the number of seconds per page to be displayed this may be changed during replay with the accelerate and decelerate icons You may also select the color for the Waveforms Background Cursor text in the scale tool and Scale Box background for the scale tool Clicking on any of these buttons will access the Color field used before see the description under General under the Multiple Windows Settings for details You also have the option to make the background of the Scale Tool transparent or opaque the Scale Box color will have no effect if the background is Tr
63. 1 Neurosoft Inc Document number 2202 Revision B options on the right mouse accessed menu list or options listed under Options on the Main Menu bar Snapshot with overlay i Clicking this button takes a snapshot of the camera view and saves it with the overlay image The figure above is a snapshot with the overlay The snapshot is saved as a bitmap bmp file The folder and file name are set using the Snapshot Path option described below Snapshot Ss Clicking this button take a snapshot bitmap of the camera image without the overlay Load Snapshot Se Use this option to retrieve a bitmap snapshot file that has been saved See the Snapshot Path option below for determining the folder and file name The Pause Resume Video button will be pressed paused when a file is retrieved Pause Resume Video Ss Use the icon to pause the video display and the resume it Right mouse button options within the video display Other features related to video are accessed by clicking the right mouse button within the video display Transparency Map Ghost Color Set Video Display Set Video Source Set Video Format Video Snapshot Video Snapshot wrOwerlay Pause Resume Snapshot Path Transparency This option determines the degree of transparency of the overlay Click it to see the following display Transparency Slide the bar for more or less transparency The higher the number the more transparent t
64. 1f the current hold value is zero and a value of is received at the parallel port then a value of 1 will be displayed as the current type value Displayed also will be the number of accepted and rejected sweeps Acquisition will terminate when the total number of requested sweeps has been stored to disk or sooner by user interrupt To pause the acquisition display in order to examine the waveforms or for other reasons click on the Pause Review Copyright 2001 Neurosoft Inc Document number 2202 Revision B button on the Tool bar clicking on the Record icon will suspend acquisition Click the icon again to resume storage to the same data file You can restart acquisition with all of the current settings by clicking on the Restart button on the Tool bar Average The Average Acquisition Type is used to obtain simple online averages files are stored with an avg extension Sweeps are initiated in the same manner as with epoch recording described above and the number of accepted rejected and individual sweep types is similarly displayed The average is displayed as it accumulates in its own Multiple Windows Display Acquisition will terminate when the total number of requested sweeps has been averaged or by user interrupt Prior to acquisition a Save File utility will allow you to enter a file name and any path preferences During acquisi tion you will see a Status box that will show the percentage of buffer space being use
65. 1s 10000 and the calibration values are 33 divide 10000 by 33 equalling approximately 30000 Try that value as the new Gain value then make minor adjustments as needed ACQUIRE4 2 160 Copyright 2001 Neurosoft Inc Document number 2202 Revision B The amplifier gain setting that you use during Calibration should be the same during acquisition i e don t change your amplifier settings after you calibrate without recalibrating Internal Calibration SynAmps Fora SynAmps click Internal and enter the desired Voltage and frequency values e g 400c and 20Hz Leave the Attenuation value at 1 Please refer to the SynAmps user manual for information on the use of the calibration option Calibration Settings Source Ge Internal Attenuation li External Sinewave Voltage pi ppl 400 Frequency Hz 20 Cancel Important note internal calibration with a SynAmps is performed with a Gain of 500 If you are using much higher gains during acquisition and wish to verify that the calibration results are correct use external calibration with a known signal Or calibrate as usual then record a known voltage signal where your Gain is the same as what you use during acquisition Then measure the peak to peak voltage of the recorded signal in EDIT to verify that the amplitude is correct After you have entered the values click OK and the calibration process will begin described below External Calibra
66. 2001 Neurosoft Inc Document number 2202 Revision B Select Amplifier AD card Neuroscan Labs Synamps Meuroscan Labs Mumps Neuroscan Labs Quicktrace Ketbdeu Metrabyte DAS 1802 Labmazter AD Neuroscan Labs Amp Simulator Cancel If you are using SynAmps NuAmps or QuickTrace highlight the appropriate selection and click OK Installation is virtually instantaneous this writes the Acquire ini file in the Windows folder Ifyou are using a Keithley Metrabyte card in the SCAN PC all new systems without SynAmps or NuAmps highlight the appropriate selection and click OK If you have an older system with a Labmaster A D card in the SCAN PC 330kHz card only SCAN 4 2 is not compatible with the 100kHz card highlight that selection The Amp Simulator can be used in place of any amplifiers and it provides a way to simulate acquisition for testing or diagnostic purposes When the SCAN 4 2 AmpInst window reappears click on Done If you wish to change the amplifiers or the A D card run the Uninstall option then run Install with the new selec tion and click Done when finished When the SCAN 4 2 AmpInst window reappears click on Done From here you may double click on the ACQUIRE icon d A from the Program Launcher to run the program ze ze wm wm H Scan 4 2 OF zl d H The small green arrow in the lower right corner of the Acquire icon lets you know that a version of AC QUIRE 1s already open Should the ACQUIRE
67. 3 averaged files to be created and saved Criteria M Enable Online Averaging l CONGRNT lt Add Average l Trials INCOM GRU M Stimulus 10 50 gt gt Del Average Response Artifact Rejection IT Enable Start ms 200 Stop ms 1500 Min uv 50 Max uv 50 Baseline Correction M Enable Start me Lan Stop mz jo Display Te Autoscale Parameters Sweeps em Start rg 200 Shop m LES Points een Avg Update Dn Start m 200 Stop mz li FUL Mm Il 1 100 Max py Do We will sort these files using the trigger type codes from STIM or other similar stimulus presentation system Click on the field to Enable the Online Averaging Click the lt lt Add Average button and a Windows Save File utility will appear Enter INCONGRU and click on Save You will see the INCONGRU name appear in the upper left box on the Sorting screen Under the Criteria section click on the field for Stimulus and enter 100 Make any changes to the settings in the Parameters Artifact Rejection Display or Baseline Correction fields These settings will affect the sorted averages and not the save CNT file Then click again on the lt lt Add Average button enter CONGRNT in the Save Files utility and click on Save Go to the Stimulus field under Criteria and enter 200 Make any changes in the fields below Then click again on the lt lt Add Average button enter NONT
68. 8 Bet lebt EE E 78 AVE SEIU ee egebeden Eet 78 EXPO SElUPGS SEU IC cicnisncsncsscusasqudauseestnaaaseacandanseisusutonaaanteatads 78 ACQUIRE4 2 3 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Bechet Ee Le EE 78 Eer 78 ele 78 Overal Ee Un E EE 19 nS SE 80 Single Window Dsplavs 80 SIE 82 DENAU DEE 84 Onine LORS carranna 85 Right mouse button options in a Single Window Ospl y 86 AGG A RE le WEE 87 Remove Uervaton 87 Add Display Elter 87 Remove Display Eiter 87 Channel Order 87 Right mouse options by clicking on the Hlechrodel abels 88 Multiple Window ueplay 89 Right Mouse Button Cptpons 90 Clear Average 90 send Waveforms to Waveboard 90 Compare Elechodes 91 Channel Hropertes cccccceccscsssssssseseeees 92 Eft e EE 93 sorted Average Window Uueplay 94 Spectral Uuepla w a A 94 Single Window Dsplavs 94 Ke 95 Display This Menu on Startup cccccceeeeee 95 A e EE 95 AMPIE S een 96 EE EE 96 Number of Channels cccccccsssssscceeeeeeeessseseees 97 FRESELE OSINIONS EE 97 ACQUISINION TYNE sro tensa ecanacistacd tate sneaa tidak 98 Speed and timing considerations for the epoch based acoutston 99 Special configuration concerns for CONTINUOUS aCOUlsSIton essences 100 Creating EEG epochs from continuous data UE 101 AE RI 101 Kleren 102 Amplifier Settings SynAmps only 102 EE 104 Bea 104 ACQUIRE4 2 4 Copyright 2001 Neurosoft Inc Docum
69. 80 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Acquire 256 channels Single Window Display a File Edit View Acquisition Options Window Help ly FA i Wiere Me tll ry PNT N SL FIC2 Ae practi aN HOMO CORT ET a ER DN ok LE Ee DE CH ae BECH F Ee ht Nace Rene GRE EN ee D SE Ent Ce KZ mm E Loes ee A c A A3 mp Les Pii F jr m Se u nie idl re AAA A DA a e 1 m J Data Not Recording Record button from Tool Bar to Start d Butter Worst OC Value Free Space MB Recording Time Event Count DU d Sg DU d S7 D0 00 28 d 20 St ooo a0 ooo ol ooal i ood os Status EI 00 00 00 Batch File ADD Avg Batch tel gt gt P il Immediate arrange For Help press F Pegel III E on user created pages The bolder vertical lines in the display delineate seconds the dashed vertical lines appear every 200ms these may be turned on or off in the Single Window Options under the Options selection on the Main menu bar discussed below The numbers appearing at the bottom of the display during acquisition indicate the stimulus and response type codes from STIM or other similar system Note that the Single Window Display may be sized relocated and closed in the same manner as other dis
70. ACQUIRE 4 2 Online Acquisition of Neurophysiological Data ACQUIRE4 2 1 Copyright 2001 Neurosoft Inc Document number 2202 Revision B pg For Technical Support If you have any questions or problems please contact Technical Support through any of the following routes If you live outside the USA or Canada and purchased your system through one of our international distributors please contact the distributor first especially if your system is under warranty In all other cases please use techsup neuro com or see the other Support options on our web site http www neuro com neuroscan support htm Or if you live in the USA or Canada please call 1 800 474 7875 International callers should use 915 587 0875 For Sales related questions please contact your local distributor or contact us at sales neuro com Copyright 2001 Neurosoft Inc All rights reserved Printed in the United States of America No part of this book may be used or reproduced in any form or by any means or stored in a database or retrieval system without prior written permission of the company Making copies of any part of this document for any purpose other than your own personal use is a violation of United States copyright laws For information contact Neuroscan Labs Neuroscan Labs A division of Neurosoft Inc 45150 Business Court Suite 100 Sterling VA 20166 6707 Telephone 703 904 9600 Fax 703 904 7870 E mail techsup neuro com
71. Deta fo Dm Epeolt haen Gel Original Maff Pee eS E E Ere FE GIE M Map5 Beaz o Dn Freq ft haen Gel Orginal Mell JE When you go to the Channel Layout display from the Edit menu you will see that the dr files will appear in the Electrode Array pull down list For this example you might wish to select the composite array and arrange the size and position of the 4 derived channels in a more prominent way During acquisition the displays might be arranged as follows with the derived bipolar Single Window Display above the raw data Single Window Display dummy amplifiers installed The Spectral Display is always linked to the raw incoming data and 1s therefore set to show the FFT results of the second Single Window Display Eee Single Window Display O Spectral data M Alpha Mil Ei Buffer 4 1240 Multiple Windows Display Free Space MB VCH a GG RG EG Adjacent is the online map displaying activity in the alpha band from the bipolar channels Lastly the 4 regional composite channels are shown in the Multiple Window Display Right mouse button options in the Single Window display You may also apply linear derivation files different filtering characteristics and reorder the channels in the Single Window display on the fly by clicking the right mouse within the display You will see the following list of options ACQUIRE4 2 86 Copyright 2001 Neurosoft Inc Document numbe
72. E Selecting this will option will show you the current version of ACQUIRE that you have installed and the serial number of your softlock This serial number is used to activate capabilities of your softlock ACQUIRE4 2 177 Copyright 2001 Neurosoft Inc Document number 2202 Revision B About ACQUIRE ACOUIRE Application Version 4 2 CH Copyright Neurosoft Inc 1986 2001 All Rights Reserved world Wide Seral number Warning This computer program ie protected by copyright laws and international treaties Unauthorized reproduction or distribution of this program or any portion of it may result in penalties and will be prosecuted to the masimum extent possible under the law Reset Workspace In previous versions of SCAN 4 0 we encountered occasional situations where the workspace file would become corrupted This resulted in a variety ofunusual operational difficulties The problems were resolved by deleting the workspace file aws extension In SCAN 4 2 we have made it easier to do the same thing Selecting the Reset Workspace option will delete the workspace file being used and restore the original default aws file You should make a backup copy of your workspace files to avoid having to recreate them if you have to use Reset Workspace References Elberling C and Don M 1984 Quality estimation of averaged auditory brainstem responses Scand Audiol 13 187 Elberling C and Wahlgreen O Estimation of audit
73. EP stimulator that may be purchased specifically for use with SCAN The first is to use the STIM Sound module to trigger the SEP stimulator and the SCAN acquisition The second method uses the SEP stimulator s internal triggering mode to control the stimulus presentation and SCAN acquisition STIM can be set to send either TTL or voltage signals and SCAN can receive either TTL or voltage signals from the stimulator You should first familiarize yourself with your stimulator s capabilities and requirements Then do you want STIM to control the stimulus presentation or do you want to use the stimulator s internal triggering If you want to use STIM the easiest configuration is to send a voltage signal from STIM to the ACQUIRE4 2 32 Copyright 2001 Neurosoft Inc Document number 2202 Revision B stimulator If you don t want to use STIM the easiest configuration is to send a TTL signal from the stimula tor to SCAN to mimic STIM s trigger Triggering from Sound The Sound module is capable of sending either a voltage pulse or a TTL signal to control the SEP stimulator Voltage trigger preferred Instead of sending a click stimulus to the headphones the signal is sent as a voltage pulse to the SEP stimulator You will need to construct a cable from the STIM audio system unit to the SEP stimulator trigger input This is aregular phone jack on the STIM side refer to your SEP stimulator to see which input jack to use and what type of c
74. Electrode Array field and select the Single 1 LDR 1020bip line if you are following the advanced part of the tutorial Resize and reposition the electrode displays as desired Then click OK Step 12 Save the Setup file by clicking Save Setup under the Fi e option from the Main menu bar or the Save button on the Toolbar use CONTSET again to add the final changes to the Setup file Step 13 To begin viewing click Acquisition on the Main menu bar followed by Start Acquisition or just click on the green triangle toward the left end of the Toolbar gt l You will see a Save File utility through which you may name the Spectral data file Enter a file name and the avg extension will be added auto matically Click Save to continue You will see a Single Window Display showing the continuous EEG ACQUIRE4 2 61 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ae sn a reg a ER Ets 7 Pals sl wi bij ie gen ANNE ER N le at Fa pret ET En D E Ee El EEN Can Ak Lea Meggie aa a fa A R Pina TEE E E EE ER ri Die ot Wen G Get Hy eda aL he ied ater each Age eh Ror A bh m ant On eg EE EA WEN p Min get DEE Pon yy Ce Dakar Abu aL ay oi abel Boa AUT LAT ey ate ie Parte MME a E ee dur e de i An oe KN dr deme dl i ai Vd d nl me fe ut AA Vu sch s Fwa De m SN ME ew AL Ap Ti pil Ta i die ie li m Shey 1 aM Ay ay eel Bus npr eh LES VR BE EA eet SEH ans a PF phate ATU Atay mut ep KE Mp Ch d V egen lt
75. Metrabyte card refer to Metrabyte documentation DMA channel Select either 5 6 or 7 The DMA channel is typically 6 If there is a conflict with other hardware select a different DMA value no physical changes to the card are required IRQ number Select either 3 5 7 10 11 or 15 for the IRQ value The IRQ is typically 10 If there is a conflict with other hardware select a different IRQ value no physical changes to the card are required Select OK to accept the settings you have entered or CANCEL to leave the setup page without making any changes LabMaster Card If you have the LabMaster AD card in the SCAN PC select that option and a LabMaster AD Setup dialog box will appear ACQUIRE4 2 74 Copyright 2001 Neurosoft Inc Document number 2202 Revision B LahbMaiter AD Semp Amphi AD Corsete Range pe PS e povai Low pars Hz 100 C AVMs High pass ball Tigger Out Vollage Dalak 7 f Active E Lu Plane address m here lgxed or EA bai Ga DHA channel E JAD mamta i Amplifier Gain Enter the gain or attenuation factor for your amplifiers for example 20000 Low pass Hz Enter your low pass filter setting that you will be using with your amplifiers frequencies above this value will be attenuated This value should correspond to the actual setting on the amplifiers High pass Hz Enter your high pass filter setting that you will be using with your amplifiers frequencie
76. Min p 100 Max pv 100 M Y Autoscale X Start ms The X Start point sets the beginning time point of the display X Stop ms The X Stop point sets the ending time point of the display Y Min vV The Y Min point sets the lower voltage limit for the display Y Max VV The Y Max point sets the upper voltage limit for the display Y Autoscale When toggled on the y axis will be scaled automatically The automatic scaling is symmetric that 1s the upper and lower limits will be the same value absolute value and are determined on the basis of the largest absolute value detected in the sweep The F mode provides an implementation of the single point F statistic estimate of signal quality developed by Elberling and Don 1984 1987 The single point F statistic F is a ratio of waveform magnitude measured against an averaged background noise Signal strength is determined over a fixed interval in which the signal is likely to occur Background ACQUIRE4 2 115 Copyright 2001 Neurosoft Inc Document number 2202 Revision B noise 1s estimated by measuring variability at a single point located anywhere within the response interval This ratio follows an F distribution with a given number of degrees of freedom Elberling and Don 1984 The F statistic can then be used to make a statistical inference as to the presence absence of an evoked response embedded within background noise The criteria and decision rules req
77. OG channels AND remove any filtering from the rest of the channels First disable the Enable field so the check mark is not there Then click the E GE S button This disables any preset filtering for all of the channels Next Deselect Al of the channels so they are red and then double click the VEOGL and HEOG channels to select only them Then enable the Enable button Select the Band Pass and enter a High Pass cutoff of 3Hz and a Low Pass cutoff of 20Hz with a 24dB rolloff from the pull down menus Then click the g SE GE S button This will apply the new settings to the artifact channels only To verify the filter settings for a given channel click the left mouse button once on the channel The current filtering parameters for that channel will be displayed in the Type Mode etc fields Band Pass Part or all of this region will be active depending on which Type of filtering you se lected above When active enter the desired High or Low pass filter setting in the window s and use the pull down arrow to access several different roll off settings in dB per octave The higher the dB octave selection the steeper the filter roll off Note the changes on the top diagram as you select different dB levels The High and Low pass values you entered will be represented by vertical lines in the diagram to see the changes click for example to the High pass field and then back to the Band Pass field Band Stop This region wi
78. RE4 2 8 Copyright 2001 Neurosoft Inc Document number 2202 Revision B It is assumed that you have a fundamental acquaintance with the operation of WINDOWS 98 NT 2000 ME The operations of the mouse path designation characteristics of windows and use of tool and status bars will not be detailed here please refer to your WINDOWS documentation for further information You should also be very familiar with the Windows Explorer Program Manager as well as functions of the right mouse button Directions in this manual are given with the assumption that you are using a mouse to make desired choices It is also possible to make many of the selections via the keyboard Many of the key words on the menu screens will have one letter underlined Selections may be made by pressing those letters on the keyboard in conjunction with the Alt key hold down the Alt key and simultaneously press the key of the underlined letter Where options on the drop down menu lists have a letter underlined typically you need only to press the letter on the keyboard Other keyboard shortcut functions that use the Ctrl key e g Ctrl p are shown on the pull down menus If you have not already installed the SCAN 4 2 software please refer to the Installation and Orientation manual Getting Started for directions It 1s essential that the software be installed within Windows using the installation CD provided from Neuroscan Labs copying the software to another c
79. RGT in the Save Files utility and click on Save Go back to the Stimulus field and enter 10 50 Note entering 10 20 30 40 50 is also valid and make any changes in the fields below ACQUIRE4 2 52 Copyright 2001 Neurosoft Inc Document number 2202 Revision B If you make a mistake in one of the files highlight the one you wish to edit and make the changes To delete a file highlight the file then click on the gt gt Del Average button After you have entered the settings you wish click on the Export button under Sort file and a Windows Save file utility will appear Enter a file name the srt extension is added automatically and click Save To retrieve a saved file click on the Import button and select the file with the Open File utility The program is set to create the three avg files based on the settings entered for each Step 9 Click the Misc tab Since this is an eyes open visually oriented task we expect considerable EOG artifact We will set the parameters for the Ocular Artifact Reduction transform here and they will be retained in all data files recorded with this setup file See the EDIT manual for an explanation of the settings for now just enter the values as shown Ocular artifact reduction Trigger av a ae Positive C Negative Threshold 10 00 Blink values Min sweeps E Channel Duration ms D EOG Step 10 It would be a good idea at this point to save the settings that you
80. Revision B Scale min max values Selecting this option displays the Map Scales screen from which you may modify the Y Min and Y Max display scale values uVs and the X Min and X Max frequency interval Hz Hap Scales Y ARIS Stop 7 8125 11 7187 Cancel Color Scale Type Selecting this option displays the Level Control Options screen The options displayed on the screen allow you to select Level Control Options Scale ype Bivalent Type f Saturation Intensity Cancel from several color schemes to use for the maps These are displayed in the EDIT manual Select an option and click OK to see the types of color schemes C Spectrum C Gray Scale Ce Bivalent Load Map File This option allows you to change map files without having to go back to the Mapping screen Selecting it will display an Open File utility from which you may select a different map file Again the map files that you use must agree in terms of the electrode labels with those in the setup file under Channel Assignments There may be fewer channels in the map file than in the setup file but not more The map files are created in MapGen Show Electrodes This option will toggle on and off the dots that represent the electrode positions as placed on the map template when it was created in MapGen Electrode Information When enabled you will see the voltage current values at each electrode
81. UAMPS ovsiems 164 Saving Viewing the Calibration Values 164 EE O e A 164 JE a 164 IRo re E E E E E E 164 E rause DISPIS ee ene enema ee eee 165 BE AOA ON E 165 Te 165 Send Waveforms to WWavelboard 165 OPON EN 165 Multiple Window Settings cccccccccccsssssseeeseeesssssseeeeeeeeessssseeeeees 166 TP Sha tee 166 WIS tee 166 CSCS E 167 Illustration of Display Harameiere 171 Single Window Settings c cssssessssssssssssssssssssssssssssesseeeseeees 173 LOJA VVOIKS le 174 DAVE tel ER Le 175 VGC E 175 Een 175 WV ele 175 E ee E E S E A T 175 TIG e eine Tu E 176 TIG VORIG AI E 176 ME 177 ACQUIRE EL E EE 177 BS ALCP IV AICI Gl ee R 177 Source ELE 177 NALS FNC UIC I coraa E A 177 POO EE 177 PSC SCE VVOINS e 178 REIT Le 178 Appendix A Network ACQUIRE ssssssssssesssnrrrnnrressseerrrrrrrrrnrnnnnseererrrrrrnrrrrnnsseeeni 179 ACQUIRE4 2 7 Copyright 2001 Neurosoft Inc Document number 2202 Revision B The ACQUIRE module Welcome to the NeuroScan ACQUIRE program a flexible high speed tool for reliable electrophysiologi cal data acquisition with multiple Online signal processing features The ACQUIRE Module records averaged epoched and continuous EEG data from up to 256 channels with the ESI system and up to 64 channels with the SCAN 4 2 system Data are acquired either from your existing analog amplifier system connected to an analog to digital A to D converter or from one or
82. able the Stimulus field check mark will apear under Criteria and enter 10 in the field The online average will trigger when a trigger type code of 1 is received 1 e all stimuli Make any changes to the Parameters Artifact Rejection Display and Baseline Correction fields Again these settings are for the sorted average file only and will not affect the EEG file that is being acquired governed the the settings under Epochs After you have entered the settings you wish click on the Export button under Sort file and a Windows Save file utility will appear Enter a file name such as SEPsort the srt extension is added automatically and click Save To retrieve a saved file click on the mport button and select the file with the Open File utility The current file will be shown in the lower field Step 9 It would be a good idea at this point to save the settings that you have made thus far Click on Save As and label the Setup file SEPSET the get extension will automatically be added Click Save then click on OK Step 10 Click on Edit again at the Main Menu bar and click on Channel Overlay You have 4 EEG channels in a line Let s resize and reposition the displays Select one of the displays and enlarge it using the standard Windows conventions grab and drag a lower corner Then click the Make Same Size button to change all the displays to that size Drag the displays to the positions you wish again using the standard Windows c
83. act are selected with this option When viewed in acquisition or in offline editing the Artifact Rejection channel labels will contain an asterisk after the label Fsp Stop You may designate which channel s you wish to have moni tored for termination criteria during the RB Averaging process Ifyou selected A under the F_ section in Overall Parameters above under Edit then you need not specify the channels again here If you selected Select in the section under Edit above then you will need to select the channels individually Click OK when you have set the channels and remember to save the setup file under File Save as Hide This option allows you to Hide electrodes on the screen display Select the channels to be hidden as described above The data for hidden channels will be recorded even though the waveforms will not appear on the screen Skip Operations This option allows you to set certain electrodes to be skipped in the data analysis process For example a setup file could include channels that are used to initiate sweeps or monitor artifact Skip channels in contrast to Bad channels are generally known in advance and are designated in the setup file prior to acquisition These channels would not normally be included in other stages of processing such as for autoscaling and in common average re referencing and may be skipped using this feature Select the channels to be Skipped as described above When viewed in acquis
84. ae KA ema a RA RENE E Segel Kaul dh An dn mn am mg gaa i d Ae daran e E E e EE ee gellen in We a a gt aa Matt deet Biaid cl Rag BRS EM Ra Ata a a a BREET Pig ee a ent Ich Gem em Vun ae ee ray Pe aie gilani ai aTi He A daa du an tt g i Wellen as a Au Pca Ay F ar rh Si d ed Asp Sedan Beer int a aL geen dk NM SEN Mu eed ia All Deen UN AA Nu Vasey ou d al Pen wt LATA p any Te Vi Wu OO KA GE Any i try a ee A AAN WO rae rata ah d lk GA pyan AAA LERA hP N YAU BAAR Aan D enen da eh Cai doar i k Nr RE Aa lie Ga i Bd ch el geb dt in a K helt e Ae D Da TT Fe lle d e en geb e db gege age ce sf d ett fe Keng eat tee emm gt dap Wan at ege Ae Wat A ege de sec Ad a k ai La gg A ally Pgh ara ar Yee Wi al Le Sr Katie engt deg den A diel Pad A ale A ake rd Ad DA hey fl Poly ice anita ee Ob 7 e SL Bis di Bif ahh i 1 Bal yh hal Far vail en cata ee VW Feu Fifer ey ny wu Sab Ve ke si Fi oR neki kasi ai 3 luet eet RARA L er earch Tears KN Men er TAAA A Be _ Ae fi Bed Kuba EN Pn Pi el ee cok Sea a dr ge at A Le en wn e P fie TE He ap e ae a a agg gag ar E gem Aoi edn e gien poe ee SN Ke daf A geet rett i me d m gem mu Wa eem emt A mme a d haet KA hina As Jk el Pace A a de a Ki be eal op emgeet get Wegen Km 9 ee Weem Age p ip wg ey rh Ct a airi ii a ay Ses ch Bee e oo E Ape Py La fee eel rg aba D ps ee feier du Ley an Ob vi Nei di Liga dra il L i N MMEN EM MM VU VE EN gu KE da Aaf Gand s ER reiege Au ft fe A Van eg L
85. al for complete operational details and warnings If enabled you will see the following display Stimulator Intensit Duration a 1000 ps 100 0 e500 ws 75 0 a 200 p5 50 0 100 ps 250 Duration i contral e50 ps 0 0 Hate singles Hz1 Hz Hz Hz ACQUIRE4 2 154 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Mlonophasic Biphasic Toggle Intensity Control Rate antral Briefly summarized set the Duration Intensity and Rate levels as desired using the sliding buttons The selections will be highlighted in blue or shown in the Intensity display Click the Monophasic Biphasic toggle button as desired Then click the Fire button to start stimulation Click the a button again to disable the stimulator and close the display Photic Stimulator Control se The photic stimulator is currently available only with Nuamps otherwise the icon will not appear Please refer to the NuAmps manual for operational details Status Bar Selection of the Status bar option displays the Help message reminder Press F1 to access the For Help press Fl Fage 0 st 000 E 12 50 ms 15 56 uV ACQUIRE Help file Brief explanations of the Toolbar options may be seen in the Status Bar by moving the mouse over the fields on the Toolbar To the right you will see additional fields These will become active when you position the mouse on one of the electrode fields in the Multiple W
86. al recordings Online averaging VEP Single sweep recording and online sorting P300 Single sweep recording and online averaging SEP Fsp averaging ABR Continuous recording online EMG audio output and online filtering N400 Continuous recording and online frequency analysis and topographical mapping Spontaneous EEG OOOOU0d The tutorials have been designed to illustrate 1 the various acquisition procedures that are possible with ACQUIRE and 2 the application of these procedures in different paradigms and modalities In a given tutorial the procedure that is being illustrated is not necessarily unique to the particular application In ACQUIRE4 2 11 Copyright 2001 Neurosoft Inc Document number 2202 Revision B other words even though you may never intend to record for example using an N400 paradigm the acquisition procedure that is described may be quite relevant to the activities you do intend to do You are strongly encouraged to read through all of the tutorials to acquaint yourself fully with the various acquisition procedures in ACQUIRE The examples below assume you are using SynAmps or the Metrabyte AD card If you are using NuAmps the interface is a little different The same steps described below still apply but there are some additional settings with NuAmps These are described in the NuAmps manual Online averaging VEP This section describes how to configure ACQUIRE for simple online averaging of
87. an evoked potential To illustrate this type of acquisition the system will be configured to acquire a visual evoked potential to a contrast reversing pattern Follow these steps to collect an online VEP Step 1 Start ACQUIRE as described above You will see the ACQUIRE main screen appear Acquae 256 channels Es De Edt Wen Genie Options Window Helo P eju m gn Tle l oo ele a oO Zz Tl Ale eRe oe ss For Help gees F F OOOO OO OOO E Step 2 The next step is to create a Setup file for the VEP recording We supply several canned setup files that may be used with Quik Caps or with Electro Caps These are called for example Cap32 ast QuikCap32 ast and so forth Click Load Setup under File or click the open file icon f amp and look in the Setup Files directory under Scan4 2 Often it is easiest to modify these files rather than create setup files from scratch To create one from scratch the best thing is to start by clicking the Make Default Setup option under Edit click OK when you see the warning The ACQUIRE program will store the most recent setup file you have loaded it will be there when you enter ACQUIRE the next time The setup file that is loaded will be displayed on the Status Bar at the lower right part of the screen GuikCap32 ast LI you do not see it you will need to increase your screen resolution For the tutorials we will begin with the QuikCap32 ast for use with the 32 channel Quik Cap an
88. and get an error message saying to input a number within a certain range click OK and see what field has been high lighted It may not be the one you had clicked Enter the appropriate number in the field that was high lighted then return to the original field you had selected and continue Note the interaction between the A D rate and the number of points selecting the A D rate in the Amplifier section will affect the sample duration and the number of points in the Epochs section In the Baseline Correction area click Enable and set the Start time to 100 and the End time to Oms Baseline correction will be performed online by computing the mean offset value from 100 to Oms and then by subtracting this value from the entire waveform In the Artifact Rejection area click Enable and set the Start time to 100ms and the End time to 500ms ACQUIRE4 2 16 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Set the Minimum value to 50uV and the Maximum value to 50uV Values anywhere in the recording epoch that exceed 50uV at the selected artifact channels described below will result in rejection of the sweep In the Display area set the X Start to 100ms the X Stop to 500ms the Y Min to 100uV and the Y Max to 100uV You will see the entire epoch displayed within the 100uV limits Leave the Y Autoscale OFF no check mark Step 8 The remainder of the tabs are not used in this demonstration It would be a good i
89. annels when using this mode The continuous update of the screen by data waveforms requires significantly more computer time than does the epoched modes of acquisition For this reason the maximum possible rate of data acquisi tion in the continuous mode will always be slower than the epoched approach The advantage to the continuous mode is that no data will be missed during the record ing In addition epochs of any size can be computed from continuous recordings There are however limits to the number of channels and digitization rates that can be achieved Fast digitization rates large numbers of channels employed in the continuous mode multiple screens and multiple online calculations are more likely to exceed the ability of your machine As a consequence errors may occur if you are running close to the limit of your machine Furthermore with the rapid evolution of installed hardware there is no way for us to anticipate what these limits may be Our philosophy has been to avoid setting the limits on acquisition and to allow the user to push the system to its limit The best way to determine how fast your system can go is to run a number of test recordings if you are interested in high acquisition rates The primary indicator that the capabilities of the system are being exceeded 1s the percentage of buffer space being used Incoming data in the ACQUIRE module are stored in a double buffer This means that while one buffer is being filled wi
90. ansparent If you have two CNT files open you may enter independent settings for it Click Save As to store the setting you have entered in the AWS workspace file then click OK to continue The Window 2 fields are used in ACQUIRE when you are displaying two single windows the second one is grayed out in EDIT Load Workspace The options that you have selected above are stored in a workspace file aws extension The workspace file also stores the sizes and positions of the various windows that you have opened You may save and recall different workspaces Use the Load Workspace option to retrieve a workspace file that you have previously created Selecting the option will display the standard Open File utility from which you may select a workspace file EDIT will retain the most recent workspace file and apply it the next time you run the program The workspace file that is being used is displayed on the right side of the Status Bar if your screen resolution is too low you may not see this ACQUIRE4 2 174 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Save Workspace The Save Workspace option is used to save the Options you have selected above as well as the sizes and positions of the data display screens you have opened You may wish to have several different workspace files depending on the operations you are performing When you have the settings and displays in a way that you would like to retain clic
91. appearing displays the Filter display contains an Jk Enable button This is necessary for removing default filter settings that may be present in the setup file It is not relevant in this example where we ACQUIRE4 2 48 Copyright 2001 Neurosoft Inc Document number 2202 Revision B are applying the filter settings to all channels Please refer to the Operating ACQUIRE section for more details Click OK to continue Enable the Sorted Average Window Display Make sure no other display options are enabled Set the polarity as desired Negative Up or Down Click OK when you are ready to continue Step 4 Now click the Amplifiers tab Set the A D rate to 500Hz SynAmps users toggle to this value 2 Continuous Next enter 31 for the number of channels don t click the Reset Positions button In this example assume that the last channel is a vertical eye movement monitoring channel recorded from sites above and below one eye bipolar referred to as VEOG and there is no HEOG channel For Acquisition type select Continuous This setting configures ACQUIRE to store data in a continuous ACQUIRE4 2 49 Copyright 2001 Neurosoft Inc Document number 2202 Revision B format If you are using SynAmps under AC DC select DC Correction with a 70 correction value If you are not using SynAmps this section will not be accessible Under Amplifier Settings we will set the VEOG channel independently from the EEG chann
92. are analyzing voltages at various frequencies The default setting is for Frequency domain check mark will appear click on the field to change to Time domain no check mark For clarification of the mapping function with Time domain recordings see the Stop section just below Start The Start and Stop fields allow you to specify the frequency limits for Frequency domain recordings or you can use the default settings Enter the starting frequency point Hz With time domain mapping the Start value should always be set to zero If you enter an integer the program will automatically enter the value corresponding to the nearest actual data point Stop Enter the stopping frequency point Hz The actual values of the Start and Stop frequencies will depend on the digitization rate and number of points Ifyou enter a Start value of 8 0 for example the calculations will use the nearest actual data point With Time domain mapping the value that is entered into the Stop field is an integer such as 1 2 etc This number designates how many data points will pass before the next map is displayed For example entering a 1 will result in anew map being constructed and displayed at every data point entering a 2 will result in maps at every 2 data points This feature is intended for use with online linear derivation computations Copyright 2001 Neurosoft Inc Document number 2202 Revision B LDR File Name You may display online maps f
93. ata files where they belong or elsewhere The Main menu bar of ACQUIRE at the top of the screen shows the menu options Acquire 64 channels File Edt View Acquisition Options Window Help 1 Below is a description of each menu option ACQUIRE4 2 77 Copyright 2001 Neurosoft Inc Document number 2202 Revision B File The File option in the main menu is used for retrieving and saving Setup files When the File option is selected in the Main menu the File menu appears The functions for each of the File menu options are listed below File Edit View Acquisition Load Setup Save Setup Export Setup as set File Load Bitmap Exit Load Setup This option is used to retrieve existing Setup files that you have created Clicking it or clicking the icon from the Toolbar Gs displays the Select Setup File display which is a standard Open Files utility window Go to the folder where your setup file is saved double click on it or select it and click Open Save Setup This option opens a standard Save As display in which you can enter a file name for your setup file to be saved and designate the folder in which you want it to be stored Click Save to save the setup file Export Setup as set File SCAN 4 0 and 4 2 use setup files with AST extensions The DOS version SCAN 3 used setup files with SET extensions There are still some utilities and third party programs that require SET files This option allows y
94. ation to the workspace file then click on OK to continue Click on Cancel to leave the page without applying any of the selections Y Axis The Y Axis page is divided into 3 regions Single Y Axis Tick marks and Scaling ACQUIRE4 2 166 Copyright 2001 Neurosoft Inc Document number 2202 Revision B us T Anis General Single Axis Tick marks W Enable W Label Ge Adjust left Shep size Auto T C Time zero Adjust right Scaling Ge Linear C Logarithmic Single Y Axis Click on the Enable field check mark will appear to display the y axis in the multiple windows displays Next select whether you would like the axis to appear at the left end of the x axis click adjust left the right end of the x axis click adjust right or intersecting with the zero point on the x axis click Time zero Tick marks Click on the Enable field if you would like tick marks displayed on the y axis Click on the Label button if you would like the labels to appear aside the scale Under the Step size option you may select the interval between successive tick marks Click on the pull down arrow indicator and highlight click the desired option range is from 0 01 uV to 100 mV or select Auto to let the program place the marks automatically Scaling Select whether you would like the scaling to be inear or using a logarithmic scale When you are satisfied with the settings click on the Save As
95. ay List Click on electrode below to add electrode to display list The current order of channels is in the left side column To remove a channel from the list just highlight it and click the Remove Item gt gt button Note this removes the channel from the list not from the display There will always be the same number of channels displayed this option will only alter the order To reorder the display completely click the Clear List button Then click the channel labels in the montage display in the order that you want them to appear As you rebuild the list you might want to separate the channels with spaces Click the ze Add Space to leave a space between the channels as you select them If you omit some channels they will be added at the bottom of the Single Window Display Click OK when you are through to see the new ordering Right mouse options by clicking on the Electrode Labels Ina Single Window display click on an electrode label from the list on the left to see the Electrode Properties display From this screen you may designate a channel on the fly as an Artifact Rejection Bad Skip and so forth channel These are the same options that you see in the Overall Param eters Channel Attributes screen ACQUIRE4 2 88 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Electrode properties l KSE Multiple Window Display In the Multiple Window Display select Multiple display click OK then click
96. ay to find the correct hold value Notice the number that is displayed n the Last Type field of the Online average box in ACQUIRE This number corresponds to the bit pattern that 1s being read from the trigger port The value that is displayed here while a sweep is spontaneously initiated is the hold value Use Inverted Values With a SynAmps and a current SoundBlaster STIM system there should be no need to Invert the triggers leave un checked When toggled on this field sets ACQUIRE to read the inverted TTL values For example a 1 will appear as 255 if inverted values 1s turned on With older STIM systems or with some other stimulation system you may need to invert the triggers As a general rule if you are not seeing any triggers in ACQUIRE try turning the Inverted Values field On or Off Also see the Test Trigger Port option described above as a tool for diagnosing triggering problems Internal selection field Metrabyte and LabMaster only With Internal triggering a synch pulse is generated according to an internal timer The synch pulse is avail able on the analog 1 output connector of the 330kHz A D module and from the Trig miniphone jack that is part of the cable from the amplifiers to the A D card for Metrabyte systems This pulse may be used to trigger external equipment Interval s The Interval value specifies the duration in seconds between internally generated sweeps Voltage selection field This field wil
97. be disabled Single Window Order This option allows you to reorder the sequence of channels on the CNT file display The same feature may be selected from the Channel Order option that appears when you click the right mouse button on a CNT file Clicking it displays the following screen ACQUIRE4 2 133 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Single Window Display Order Ordered Display List Click on electrode below to add electrode to display list The current order of channels is in the left side column To remove a channel from the list just highlight it and click the Remove Item gt gt button Note this removes the channel from the list not from the display There will always be the same number of channels displayed this option will only alter the order To reorder the display completely click the Clear List button Then click the channel labels in the montage display in the order that you want them to appear As you rebuild the list you might want to separate the channels with spaces Click the ze Add Space to leave a space between the channels as you select them If you omit some channels they will be added at the bottom of the Single Window Display Click OK when you are through to see the new ordering To save the CNT file with the new order click Save As under File You will then have the option of either overwriting the existing file or creating a new one Electrode Notice first in t
98. bottom one Toolbar options are next followed by any remaining options Please use the Table of Contents to find desired sections most quickly Note that this manual also exists in PDF format in the PDF folder under Scan4 2 It may be accessed from within the ACQUIRE software by clicking on Help Hel and then ACQUIRE Manual Gs Acquire Manual Installation of Amplifiers and AD Card Prior to operating ACQUIRE you should first complete the software installation of the amplifiers and or A D card that you will be using If you are not sure whether your amplifiers and A D card have been installed go into ACQUIRE described below click on Edit from the Main menu bar and look for an option saying SynAmps Hardware Metrabyte DAS 1800 Amplifier or Lab Master A D Amplifier below the Make default option Ifinstead of one of these you have the Amplifier Simulator line you need to complete the installation To install the amplifiers and or A D card you must run the AMPINSTALL program To do this run GA Ampinst from the Windows Explorer program The SCAN 4 2 AmpInst display will appear SCAN 4 2 Amplnst Uninstall Name f Net Acquire Broadcaster C Net Acquire Receiver The Network section is relevant only if your are running ACQUIRE across a network see the appendix at the end of this manual for details Click Install and the Select Amplifier and A D card window will appear ACQUIRE4 2 65 Copyright
99. bove The next three options involve sending waveforms from the online average files automatically to the Waveboard Waveboard W Auto Add Last I Auto Add en Add After Auto Add Last When enabled this option will automatically send the final waveforms from the AVG file to the Waveboard Auto Add The Auto Add and Add After options are linked When enabled the Auto Add options will automatically send waveforms from the building AVG file to the Waveboard after every X number of sweeps Enter the desired number of sweeps in the Add After field For example if you have selected the Auto Add option and have entered 10 in the Add After field the average of the first 10 sweeps will be sent to the Waveboard automatically Then the average of the next 10 will be sent and so forth If you also enabled the Auto Add option the final averaged waveforms will be sent automatically to the Waveboard If your total number of sweeps is a multiple of the number in the Add After field then the last two waveforms sent to the Waveboard should be identical Note If for example you are sending the average of every five sweeps to the Waveboard and there are 40 sweeps you will only see 7 wave forms on the Waveboard You will need 41 sweeps to have the last sub average sent Add After Refer to the Auto Add description above for the use of this option Select this option by clicking on the tab portion of the index card at the Triggers label
100. cate that you are recording from more than 32 channels or 1f you have created additional display pages in the Individual Channel Settings section Clicking on this field or on the corresponding icon on the tool bar will cycle forward through the pages you have created Previous Display Page Clicking on this field or the corresponding icon on the tool bar will allow you to cycle backward through the electrode display fields or if you have created additional display pages in the Individual Channel Settings section The Next and Previous Display Page options will not be accessible if you have not created additional display pages Baseline Corrections The baseline button is used to center the baseline of waveforms graphically with their associated labels located on the left hand side of the screen This operation is performed each time the wave forms begin drawing from the left hand side of the screen and has no effect on the actual data This option is implemented as a toggle The baseline option will toggle on and off each time you press the field or select the corresponding icon from the tool bar Baseline is particularly useful with D C recordings in which the D C values will cause a large offset of the starting screen location Invert Polarity on Display The Invert option will invert the polarity of the signals on the display screens so that positive voltage is plotted up This option is most easily accessed from the corresponding icon on
101. cccccccceceeeeeeeeeeeeeeeeeeeeees 140 WIKIS TIE SN E 141 SEU 142 synAmps Hardware NuAmps Metrabyte card or LabMaster card 143 Ehe 143 BIR ee RE eee eee 144 nlf Decelerate Display cceseeceeeseeeeeeeeeeeeeeeeeeeneeeeseseeeeneeeneeens 144 Be Increase SCANNING DE 144 1 Ree E 145 r eNO cle E 145 g4 Next Display Haoe ENNEN 145 gt Previous Display Hage 145 HE Baseline COMCCUON viccsnvesseveseessccneseteedererseteicesevecteteavetervedesoenness 145 A Invert Polarity on splay 145 pc Perform DC Correction sesesseeessseseseeeessseseeeeeeeeeeees 145 le ele 146 t Video Capabilities in ACQUIRE cccceeeseeesseessseeeeees 148 Launch Vaveboard AAA 154 Bee Launch Montage Foot cccscssesesseesssessseesseessseesseeesees 154 i OURO E eee ee ne ne ee ee ene eee eee ees 154 SEP Stimulator Control eee eee eee eee eter eeneeeeeee 154 ga Photic Stimulator Control 155 SELCA eege 155 Eegiel 155 WAN CID ON erst ce E E E S 155 Viele sine A eee eee eee ee eee ee 156 PRC AUIS HOM BE 156 PS Stant ACQUISINON edzesereccseecesaceetececttvectieaesecensysensenet enn iE 156 oec eie e E E E E T 157 i Impedance of Negative Electrodes EE 158 pig L a llbtraion 158 Internal Calibration LabMaster and Metrabyte 159 Internal Calibration Gvn mpe le 161 ACQUIRE4 2 6 Copyright 2001 Neurosoft Inc Document number 2202 Revision B External Calbraton 161 Calibration N
102. consists of entering the new values selecting the channels that you wish to modify and then applying the modifications In practice you may find it easier to select the channels then enter the new settings and apply them Either method will work Amplifier Settings Selected Channel Gain Range 11 mu DCCUIaCH 0 168 p DP Low pass 30Hz High pass 0 15 Hz Apply To All Selected Channels The right side part of the window is used to select the channels that you wish to modify The electrode labels and their positions on the screen are ACQUIRE4 2 102 Copyright 2001 Neurosoft Inc Document number 2202 Revision B all user determined The Channel Assigment List described below is perhaps the easiest place to enter the electrode labels although these can be modified in other places as described below The positions of the electrode displays are created in the Channel Layout feature described below If you are using one of the preset setup files the labels and positions have already been set using the Channel Assignment and Channel Layout features Many people prefer to make their own setup files using their own labels and display positions Channels can be selected or deselected by double clicking the mouse ona channel green is selected and red is deselected or you can use the Select All Deselect All SelectAll and DeselectAll buttons to affect all channels A single selected channel will show
103. ct a color for each frequency band Note that these settings apply only to the Online Frequency Analysis you selected under Edit Overall Parameters Frequency The default frequency band names are Delta Theta Alpha Betal Beta2 Muscle 50Hz and 60Hz however you may rename these as you desire ACQUIRE4 2 169 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 170 x Frequency Band Colors Name Start Hz Stop Hz Color Band 1 p EE Band 1 Band2 Thea hb 7 38 band Band3 aha Rham Band E Bandd Bea bn Jam Band Banas Bete2 fo Jan Band Bande Muscle fo 500 Bande Band 50H fs ft Band Bands m fs ot Band P Cancel i You may also redefine the frequency limits ofeach band Keep in mind that the actual frequency resolution will be determined by the Points value you selected under Edit Overall Parameters Frequency and the AD rate For example 256 points with a sampling rate of 1000Hz gives a frequency resolution of approximately 3 9Hz Therefore while you might define for example Alpha as 8 0 to 12 99Hz the actual Start and Stop values may be closer to 7 8Hz and 11 7Hz With 1024 points the actual Start and Stop points are approximately 7 84 and 12 74 respectively The program will round the Start and Stop points you enter but realize that these are not the exact values that are calculated Notice also that you may have overlapping frequency bands Fo
104. ction W Enable Start rns E UU End rms E assume that you want a 100ms pre stimulus interval and a 500ms post stimulus span with automatic artifact rejection and baseline correction enabled during acquisition In the area labeled General set the number of Sweeps to 200 This should be more than adequate to provide a good signal to noise ratio for the contrast reversal VEP Set the Start time for 100 pre stimulus and the end time for 500ms The contrast reversal VEP has components that peak before and after 100ms The pre stimulus interval will provide a stable baseline that can be used to remove any unwanted offset The post stimulus interval of 5 00ms should be of sufficient duration to include any late components of the evoked response Click on the Points field and set it to 601 if not already set In this example a total sample interval of 601 ms 100ms pre stimulus and 500ms post stimulus plus the zero point has been selected The stimulation protocol therefore must take this into consideration in order to provide at least this interval plus the overhead of the computer A stimulation rate of 1 per second 1s recommended in this example If faster stimulation rates are desired you can decrease the sample interval as long as the post stimulus interval is of sufficient duration to view the major response components 1 e P100 The program is designed not to allow invalid settings for any of the fields If you click on a field
105. d the free space available on your hard drive recording time the number of accepted and rejected sweeps the trigger type code of the last sweep and the percent complete Percent Complete is based on the number of Sweeps you selected in the General section on the Epochs screen under Overall Parameters Right mouse buttons options If you have an online average displayed including online sorted averages position the mouse between the electrode channel displays and click the right mouse button You will see the following display Clear Average Send Waveforms to Waveboard Clear Average Select this option to clear the online average and restart the averaging process Send Waveforms to Waveboard Select this option to send the waveforms to the Waveboard F Average The F mode can be used to make a statistical inference as to the presence absence of an evoked potential embedded within background noise Its primary use has traditionally been with brain stem EPs but may have validity for other EP components It is described in more detail below Speed and timing considerations for the epoch based acquisition Labmaster and Metrabyte systems only In the epoched based acquisition modes Average Epoched and F _ Average the system will sample for a specified period of time and then perform a number of operations depending on the mode and options selected These operations require computer time How much time is required depends on
106. d modify it according to the various acquisition methods we will demonstrate Step 3 Once you have retrieved the QuikCap32 ast default or other setup file click Edit from the Main Menu bar then click Overall Parameters ACQUIRE4 2 12 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Overall Parameters A multi card window will appear with the Startup card on top For this recording we ll look simply at the incoming signals from all channels as the average builds Enable the Multiple Windows Display check mark She En ery ren eT will appear Make sure no other displays are enabled Click the Auto Save field On check mark will appear and averages will be saved automatically Set the Negative polarity to be Up or Down as desired Step 4 Now click on the Amplifiers tab Set the A to D rate to 1000Hz click on pull down arrow to the right of the A D field and click on 1000 using the up and down arrows if needed to find it ACQUIRE4 2 13 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Acquistion Amplifier Settings A D Date om Number of Channels 32 Reset Positions Acquisition Type Selected Channel i Gain 1000 M Average Range SEN AC DC AC fe DC DC Correction W Enable E Level Motch T Enable Frequency Accuracy 0 084 p LSE Low pass 1OU0Hz High pass 1 00 Hz Apply To All Selected Channels
107. d on other chan nels e the Bad is independent from the other channels then the operation will ignore the Bad or Skip settings X Units This option allows you to enter a label for values along the x axis You may set the label independently for each channel the channel that is displayed in the Selected Channel field is the one that you are setting The Default label is ms If you change this and wish to return to the default label later you must enter the word Default with a capital D Y Units This option allows you to enter a label for values along the y axis You may set the label independently for each channel the channel that is displayed in the Selected Channel field is the one that you are setting The Default label is uV If you change this and wish to return to the default label later you must enter the word Default with a capital D Colors The Colors area allows you to set the waveform colors indepen dently for each channel Further you can set the new colors to appear only ina Single Window Display or only in a Multiple Window Colors Single Mul Ge Default C Default C Custom e Custom E Display or both The Default setting is the color you specified under Options Multiple Window Settings General Waveform button Click the Custom radio field to access the standard Colors palette and select a color for the specified channels Select the channels to be modified in the same way as described a
108. d then click on the Derivation File button This will access an Open File utility through which you may select a Linear Derivation file dr extension that you created either in the Montage Editor or with your text editor please see the EDIT module for a more ACQUIRE4 2 84 Copyright 2001 Neurosoft Inc Document number 2202 Revision B complete description of linear derivation files as well as Appendix A at the end of the EDIT manual After you select the desired file it will appear in the field at the far right of the Startup window You should decide which type of Display you want Single or Multiple and set up the Derivation file accordingly You may have up to 3 dr files ata time Note the additional display pages you may have created will not be available if you apply and LDR file Online LDRs LDR files that you create in the Montage Editor or a text editor can be used for online recording in ACQUIRE Inthe example below we are using the Idr files that are described in the beginning of the Montage Editor appendix at the end of the EDIT manual to demonstrate some simple online applications Let s say that we would like to display the raw data in a Single Window Display a bipolar montage in a Single Window Display the regional composite channels in a Multiple Window display and that we would like to have an online map for alpha based on the derived bipolar channels The only additional file we will need is a map
109. de Waveboard Skip Operations Auto Add Last Bad Auto Add Default o Units id Add After Default Y Units Select All Deselect Al Step 6 Triggering for the VEP will be controlled by an external device e g the STIM system Click on the Triggers tab to access this option then click the External field Startup Amplifiers Channel Attributes Triggers Ge External Hold value lo IT Use inverted values E nemal Intersale The Hold value should be set to 0 in most situations see the Triggering section in the Operating ACQUIRE section below for special circumstances The Use Inverted Values field is either checked or unchecked depending on your particular system if you do not see triggers with it set one way try it the other way The display above is seen when you have a SynAmps Invert disabled Ifyou are using different amplifiers or acquisition modes the other fields will be active refer to the Operating ACQUIRE section below for more details ACQUIRE4 2 15 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Step 7 Click on the Epochs label to set the parameters of the recording epoch For this example General Artifact Rejection Display Sweeps 200 MW Enable Start ma 100 Start ms 100 Start ms E OU Stop ms FOO End ms 500 Points en Avg Update i End m 1500 Mm 100 Mm p Lon May yh 50 Y Max 100 IT Y Autoscale Baseline Corre
110. de The Nuamp amplifiers are different from most other amplifiers The main point to understand is that the reference electrode is computed in the software That means there are few limits on what can be used for the reference You can select a single electrode used linked or single ears and so forth Let s say you want a linked ears reference That is the first option A1 A2 2 Click that button and you will see the current selection written at the top of the display Note that you have the options to display the head contour and the channel numbers at the bottom of the display Current selection Linked ears Reference options As mentioned you have a great deal of flexibility in the selection of the site s for use as the reference To select a single channel such as Cz just click the Cz field You will see the choice indicated in the top line of the display Set reference Current selection Cz The other options are A1 A2 2 This is the linked ears option Left gt A2 Right gt A1 This option uses the contralateral ear mastoid as the reference Right gt A2 Left gt A1 This option uses the ipsilateral ear mastoid as the reference AV means Averaged electrode In this case all specified electrodes are recorded and the averaged potential is calculated and subtracted from original signals Only actually placed electrodes are used For example if you specify 10 electrodes to be recorded but only place 8 of them then only
111. de the late components Click on the Points field it should change automatically to 551 The Avg Update field is not used for this recording In this example a total sample interval of 1101ms 100ms pre stimulus 1000ms post stimulus and the zero point has been selected The stimulation protocol must therefore provide at least this interval plus addi tional time for the overhead processing of the computer A stimulation rate of 2 per second should easily be adequate in this example If faster stimulation rates are desired you can decrease the sample interval as long as the post stimulus interval is of sufficient duration to view the major response components 1 e late positive negative complex The program is designed not to allow invalid settings for any of the fields If you click on a field and get an error message saying to input a number within a certain range click OK and see what field has been high lighted It may not be the one you had clicked Enter the appropriate number in the field that was high lighted then return to the original field you had selected and continue Note the interaction between the A D rate and the number of points selecting the A D rate in the Amplifier section will affect the sample duration and the number of points in the Epochs section In the Baseline Correction area click Enable and set the Start time to 100 and the End time to Oms Baseline correction will be performed online by computing the mean offs
112. dea at this point to save the settings that you have made thus far Click on Save As and label the Setup file VEPSET the ast extension will automatically be added Then click OK Step 9 For the sake of demonstration let s look at some other features Go back to the Edit option on the Main Menu bar and select Channel Layout You will see the 32 channels laid out in a preset display You can use this screen to reposition the electrode displays change their sizes rename the electrodes import electrode position information and so forth Let s take a look at some of these features Acquire 256 channels Channel Layout Et ol OK amp Pa d a aw Fille Edt View Acquisitia Electrode Array Original array o h um t Size and Position 10 20 Positions Reset Positions Make Same Size Import Positions Export Positions Adjust Positions Display Page Page E Assam hanne E Single Window Order For Help press F Ifyou wish you may resize and reposition the individual channel displays Select one display and click drag a corner to resize the display then click the Make Same Size button to make all of the displays the same size Move the displays around the screen by grabbing the colored bar at the top where the electrode label is and dragging the display to a new location Note Be sure to click OK and Save the setup file before you click the Make the Same Size button aga
113. ds are empty then there is a communication problem with the SynAmp s For Scan4 you should install the current versions of EC and DSPSYNAM If these were not included on your installation CD they may be obtained from the NeuroScan Web or FTP site or by contact ing technical support The new files should be placed in a known directory such as the Scan4 directory Then click on the Update Drivers button 1n the Diagnostics section and the Update Drivers window will appear ACQUIRE4 2 67 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Update Drivers C Seralcable Comi Com Ce SCSI h Reboot when finished Progress Status IER Total blacks D Current Block D Stop Update Cancel Click on the Browse button and find and select the Ec exe program The file will be in the Scan4 2 SynAmps Disks S YNDISKS DISK2 EC folder The preferred method of transmission is over the SCSI cable Click SCSI and check the Reboot when finished box if you are updating more than one file or more than one SynAmps check this option when you perform the final update Then click Update and watch the progress of the transmission Repeat the same process for the Dspsynam file Repeat the process for additional SynAmps As of approximately February 2001 new SynAmps no longer have the serial cable download capability you must use the SCSI method See the SynAmps manual for download directions using the serial cable
114. e when you are using the ear insert earphones the air conduc tion of the sound introduces a latency increase of Ims 146 eA alalla 5 HAAG 9H HB H HAdE H HH HANNING Bee vr vr aBa H Negative H Step 7 Click on the Epochs label to set the parameters of the recording epoch For this example assume that you want a 5ms pre stimulus interval a 1Sms post stimulus span with automatic baseline correction ACQUIRE4 2 43 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Display Start rz 5 Stop me General Artifact Rejection Sweeps om Stark ma 5 End rms is Fonts om Avg Update Dn W Enable Start rng 5 End ms i 5 Y Min Mm p Lon May p 50 Y Max IT Y Autoscale Baseline Correction W Enable Start ms L End rm E and artifact rejection enabled during acquisition In the area labeled General set the number of Sweeps to 20000 Enter 5 for the Start point and 15 for the End point Click on the Points field and set it to 400 points ifneeded The Avg Update field is not applicable to Fsp recordings In this example a total sample interval of 21ms Sms pre stimulus 15ms post stimulus and the zero point has been selected The stimulation rate must therefore provide at least this interval plus additional time for the processing overhead of the computer A stimulation rate of approximately 15 20 per second 1
115. e clock does not stop when acquisition 1s paused Event Count This field displays the accumulated count of events in the file such as the number of triggers and responses sent from STIM Filter Adjacent to the Single and Multiple Window Enable fields are the Iv Filter Display fields When you click on one of these the Set Display Filter button will become active Click it and the Filter window will be displayed allowing you to select various online filtering parameters ACQUIRE4 2 82 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Type i Low pass High pass Ce Band Pass C Band Stop Band Pass High Hz fi E db Low Hz E E dB sl Channels Je W Enable Apply To All Selected Channels gt Rectify The Filter window is divided into 7 main sections the graphic display Type Mode Channels Band Pass Band Stop and the montage display The graphic display at the top of the window shows the results ofthe settings you enter Type You may select one of the following options Low pass filtering only passes frequencies below the inputted setting attenuates faster frequencies Clicking this option activates the Low Hz field in the Band Pass section below High pass filtering only passes frequencies above the inputted setting or attenuates slower frequencies Clicking this option activates the High Hz field in the Band Pass section below
116. e date Dm mek Bie ahd ahah Cd pen ah gg deg eet sods bat Ray eee r es ee keet eia i Ma Sp oe bp ey Eet ANEN E te i a a 9 E ai s eich et Reg alte ila ek d Beni we in eal lial a erime M K EN ee T inana dd a aji d em A N T i nts ah dE EN Emmi AH e e eet ve Kelt mm ie amgang ee hehe Fus BREN EGO A e A pe teg E e e a d gg et Let EEN ag Tiaa BOGE Aai sp ea ar Cp K J eh Del Wen AA d ALF a Oe DA Fite Fa F n Hal pees FH Pa 1 end meng ii W w signals a Single Window Display showing the derived bipolar channels if you are following this part of the tutorial map displays for Delta and Alpha as well as the online Spectral data Since we set the online FFT for ensemble with an update of every 5 sweeps this will take a few seconds for display If you do not see any results in the Spectral window increase the scaling with the Up arrow on the Toolbar As soon as you click a scaling arrow highlight a display etc you will see the Active Map display appear This will have buttons available for each map that 1s displayed Delta Theta etc You must have at least two online maps for the display to appear Active Map To understand the function of the Active Map buttons you should first enlarge one of the channels in the Spectral Data display to full size You will see in addition to the power spectrum display displayed as lines rather than a histogram in this example two vertical dashed lines and two hor
117. e of the calibration signal sent from the AD card typi cally 1V attenuator is the attenuation factor you apply to the AD output typically 20000 and amplification is the actual gain of your amplifiers variable but 20000 works well In this ex ample 1V 20000 X 20000 1V ACQUIRE is expecting an approximate 1V 8 to 1 2 Vs continuous sine wave for all channels If your output is outside of this range modify the amplifier gain AD output voltage and or the attenuation factor to get it in range How this information 1s entered into the ACQUIRE program 1s described below To use the SCAN internal calibration signal Metrabyte or LabMaster A D cards click on Calibra tion under the Acquisition menu and the Calibration Settings dialogue box will appear Select the Internal option Enter the Attenuation value e g 20000 and enter the AD output voltage in the Sinewave Voltage field in microvolts peak to peak Fora 1V signal enter 1000000 Note that ACQUIRE4 2 159 Copyright 2001 Neurosoft Inc Document number 2202 Revision B you can enter the exact voltage going into the headbox such as 50uc with an Attenuator value of 1 and the result will be the same Also enter for example 10Hz for the calibration signal fre quency Calibration Settings Source Ce Interne Attenuation 20000 External Sinewave voltage WW pp Dom Frequency Hz 10 V Cancel The amplification or actua
118. e online Spectral Display and uses the same update settings You may show up to 5 maps at atime The box before Map 1 Map 2 etc enables a map check mark will appear when enabled The following parameters are set for each map and will vary with the A D rate and Points ACQUIRE4 2 123 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Label Mm Max Domain Start Stop LDA File Name Map Fie Hame Data Source Video If Mapi Deta o o E bealt Page Der Original Map cap32 Raw sl E IT Map 2 Theta jo ro RI Freq 3 906 7 812 Fe Original DE Ran gt M Map 3 alpha jo E E bealzaz 11 71 De Original Map cap32 Raw sl r Map5 Bea2 D Dn Si Freq 19 63 39 05 ey Original Fen Faw 2 p M Mapa paat E po reg p bag pew pia Mao fan e e ACQUIRE4 2 124 Hone Label Enter a label for the map you have enabled or use the default ones Min For Min enter the lower scale value for the display or use the default settings for frequency domain mapping this would typically be 0 Max For Max enter the higher scale value for the display or use the default settings for typical EP recordings the Min and Max might be 15 and 15 respec tively Domain This field allows you to select between time and frequency domain mapping time domain is used with EP recordings where you are analyzing voltage changes across the epoch frequency domain is used primarily with EEG recordings where you
119. ect this option the waveforms in the online average file will be sent to the Waveboard It 1s not necessary to open the Waveboard first 1t will open automatically ACQUIRE4 2 90 Copyright 2001 Neurosoft Inc Document number 2202 Revision B If you position the mouse inside an electrode display and click the right mouse button you will see the following option Compare Electrode s Channel Properties Butterfly Plot Compare Electrode s Click on this option and you will see the following window left side of display Comparison list Electrodes Current Electrode gd Add EK Del Cancel This feature allows you to select electrodes to superimpose on the original electrode display In the Electrodes column highlight an electrode of interest and click the lt dd button The electrode will move to the Comparison List column Alternatively you can just click on the desired electrode s on the right side montage display ACQUIRE4 2 91 Copyright 2001 Neurosoft Inc Document number 2202 Revision B You also have the option to change the color or line style of any electrode in the list by clicking on the button or by selecting one of the line style options from the Line pull down menu Added electrodes can be deleted by highlighting the electrode and then clicking on the gt gt Del button The Current Electrode field indicates the display you originally chose with the right mouse button The
120. elect either 10 11 or 12 for the IRQ value The IRQ is typically 10 If there is a conflict with other hardware select a different IRQ value no physical changes to the card required Select OK to accept the settings you have entered or CANCEL to leave the setup page without making any changes ACQUIRE4 2 76 Copyright 2001 Neurosoft Inc Document number 2202 Revision B The ACQUIRE Program For SynAmps users always make sure the SynAmp s is turned ON and that SN1 and SN2 SN3 etc appears BEFORE you boot your PC To start ACQUIRE first double click the SCAN 4 2 icon and the Program Launcher will appear d e Scan 4 2 d Start the ACQUIRE program by clicking on the ACQUIRE icon from the Program Launcher The main screen will appear Acquire 256 channels Ill File Edit View Acquisition Options Window Help For Help press F Pages If you purchased a system with 64 or fewer channels the top line of the display will show ACQUIRE 64 channels If youhavea 128 channel system that will be indicated This lets you see the largest number of channels that your system may record Sticky pins As you go through the various displays in ACQUIRE you will encounter an icon in the upper right hand corner is some displays that may be new to you This is the Sticky Pin The Butter I Toggle Stickyness Status boxes have them They are convenient devices to make the Status boxes stick with the d
121. els SynAmps only Note We recommend using the same Gain and Filter setings for the VEOG channel as used with the EEG channels if you are planning to use the Ocular Artifact Reduction transform in EDIT Notice that there is an HEOG channel but no VEOG channel if you started with the QCap32 ast file That is because we omitted one channel by entering 31 and the VEOG channel was the last one We will rename it shortly For now we will change the Gain on the VEOG HEOG channel Click the Select Al button and enter a Gain of 500 a Low Pass filter setting of 30Hz and a High Pass filter of DC Then click the see Ge A button Now click the Deselect All button and the double click the HEOG channel so it is the only green one Change the Gain to 250 and click the rea jaan button to change the Gain on that channel only Then click OK For non SynAmps systems these values are set in the Metrabyte or LabMaster option under Edit from the Main Menu bar These settings should not be changed unless you physically reset the amplifiers and recalibrate with those settings Leave the Notch filter OFF no check mark in the field Step 5 Now click the Channel Attributes tab Rename the HEOG channel to be the VEOG channel Click on it once to select it then change the label in the Selected Channel field For this recording let s set FP 1 2 F7 8 and the VEOG channel to be Artifact Rejection channels Do this by double clicking these channels so
122. en acquisitions to verify that the resistances are still low and comparable across channels You also have the option to mark as Bad or Hide channels any channels where the impedance exceeds a threshold you set Go to Edit Overall Parameters and the Misc tab to see these Impedance selections Impedance operations These options will automatically set channels as either Bad or Hide channels when the impedance level exceeds the threshold value you enter in the Impedance limit kOhms Impedance limit field E This is particularly useful in 128 or 256 channel recordings where it is not unusual to have a few channels with high impedances The M ark high Hide High buttons Impedance impedance when clicked will set the high impedance channels channels as bad channels as Bad channels which will exclude them from many of the offline analyses in EDIT OR as Hide channels which will hide those channels from the display the data are still recorded You may select either option but not both The Prompt after impedance check Option when enabled will prompt you to use the options Impedance of Negative Electrodes With bipolar recordings there are positive and negative electrodes depending on how they are inserted in the headbox The default measurement is for the positive electrode which is the active electrode for unipolar recordings Use this option to measure the impedance of the negative electrodes during Impedance testing
123. ent number 2202 Revision B FION SE 104 Channel ARDUS ee Ee 105 Selecting Channels ek 105 POD ut EE 106 Selected Channel 106 Physical Channel 106 CAM FAO DEE 106 HTP COA CO EE 106 Artifact Heiechon 107 FSD OD nnana a 107 FCC E E EAEE A A aenGuieG 107 SKIP Operati NS eusian a 107 e BEE 107 A UNS eene 108 YUNG aa sane teen ena ae ane 108 ele EE 108 Ate Ee Le EC 108 PAULO E elle DEE 109 AGA ANG onnenn tinea 109 TOGO aee nner eer need Dene en nr se Nanny omen nea art een ain 109 External selection field 110 Internal selection feld 110 Voltage selection feld 110 E DOCH S EE 111 ERT EE 112 Baseline Correction ann 113 Ariat ROJECUON pni te era eee ne i cee 114 RI 6 Fe EE 115 Si NEE 115 Terminate Mead 116 Terminate Channel 117 EE ee 117 RI 6 Fe EE 118 Kell le E 119 SCAN DE 119 Varela e 119 Beien O EE 120 ACQUISINONIVIOGE EE 120 WIS DIAY EE 120 SONIN DEE 121 CHILE EE 122 Parameters Artifact Rejection Display and Baseline Correchon 122 SOME ME nei 122 AUO isco pict ce eel wi esse at ect ane oo 122 MAODIR EE 123 Time domain file mappimg 126 ACQUIRE4 2 5 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Eeer 129 INNS Cgc E eee E AE A A E 130 Channel er eee ner ete nn ar nett ET ete nner are een 131 EICOTOGC AN Iy ene eae eee ne en ee eee 132 RI 6 Fe A le 132 Single Window Crder ENEE 133 EE 134 Ze AG OOM E 135 Channel Assignment Table c ccccsc
124. er in the field that was high lighted then return to the original field you had selected and continue Note the interaction between the A D rate and the number of points selecting the A D rate in the Amplifier section will affect the sample duration and the number of points in the Epochs section Disable the Baseline Correction option by clicking the Enable field so that the check does not appear Disable the Artifact Rejection feature by clicking on the Enable field so that the check does not appear Step amp For this recording it might be useful to monitor the online EEG spectral analysis Click on the Frequency tab then click on the Enable field Note that these options are only available when you have selected the continuous or epoched modes for Acquisition Type under Amplifiers ACQUIRE4 2 59 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Startup Amplifiers Channel Attributes Triggers Epoche Fap Average Frequency Sorting Aut M Enable Online Frequency Analysis Scaling Ce Amplitude Acquisition Mode Display Ge Ensemble Average f Histogram Sweeps Jh Save C Individual Sweep C Power C Line Herber ft Start Hz jo Stop Hz jo YMin E amas T ina Running Average smacthing 2 Window Paints Al Resolution 0 488281 Length 2 jo Range 125 M Y Autoscale Duration Type Cosine d i These options are explained more fully
125. er to previous figure When you select Overall Parameters under Edit you will see the Overall Parameters window The Startup display is the top card It is divided into 6 main areas the Single Window Display No 1 the Single Window Display No 2 the Multiple Window Display the Sorted Average Window Display the Spectral Display and Polarity Single Window Displays The Single Window Displays are divided into 3 parts the Enable field Filter Display and Linear Derivation Click on the Enable field click box so that check mark Single Window Display No 1 Enable Filter Display Derivation Derivation File appears in Single Window Display No 1 and the Single Window Display No 2 and the Spectral Display fields become active The purpose ofthe 2 single window displays is to allow you to monitor incoming data in more than one way For example you could create a linear derivation file described below in which incoming unipolar channels are recomputed for a bipolar montage You could observe both montages during acquisition Or you could apply different filtering to the display described below or change speeds or scaling and monitor both Clicking on the Single Window Display followed by OK and the green Start Data Acquisition arrow activates a scrolling EEG paper like display of the on going signals The channels are displayed in a vertical fashion additional channels or subsets of channels may be displayed ACQUIRE4 2
126. ers to the other Higher numbers use the higher order bits of the port For ACQUIRE4 2 33 Copyright 2001 Neurosoft Inc Document number 2202 Revision B example the number 64 corresponds to pin 8 on the back of the STIM connector Contact Neuroscan Labs technical support if you need assistance in building this cable Triggering from the SEP stimulator You can bypass STIM and use the SEP stimulator to control triggering of acquisition SCAN can receive either TTL or voltage triggers TTL triggers Having the stimulator send TTL signals is preferred because the procedure mimics the STIM system trigger settings within ACQUIRE are the same as those for use with STIM External triggers with Hold value of 0 Invert On or Off depending You will need to construct a cable to replace the STIM to SCAN cable on the SCAN side with whatever connector you need for the stimulator connection This can be a much simplified cable typically carrying a single bit for a trigger of 1 Contact Neuroscan Labs technical support if you need assistance with the cable With some SEP stimulators we have found that the duration of the TTL pulse is too short to be recognized by the SynAmps In that case you will need to build a pulse stretcher or you may obtain one from Neuroscan Labs Step 7 Click on the Epochs label of the display card to set parameters of the recording epoch For General Sweep foo Start m on End ms Dm Points Dm Avg
127. es are 33 divide 10000 by 33 equalling approximately 30000 Try that value as the new Gain value then make minor adjustments as needed After setting the values on the calibration screen click OK This screen will be replaced with a ACQUIRE4 2 162 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Multiple Display Window with displays corresponding to the electrode channels You will see sequential sweeps of the sine wave After about 10 sweeps you will see a screen like the Calibration S AA CA 1044010 AWN DU Es 1051071 alibration one shown You will be asked whether or not to accept the calibration values The values referred to are the numbers in the upper right hand corner of each channel display box When calibrated properly these numbers should all closely approximate 1 0 plus minus approximately 0 05 Beware of calibration values that equal 1 000 at all channels This is an indication that the Gain setting in ACQUIRE does not match the actual amplifier gain Watch also for clipped or partially out of range sine wave signals seen during the calibration process the amplifier output is too great The sine waves should appear as in the figure above The calibration values are simply multipliers used to correct any minor deviations among channels The signals received from the amplifier are multiplied by the calibration values to obtain the true values used throughout SCAN The calibration va
128. et to 0 see the triggering section in the Operating ACQUIRE section below for special circumstances If you have a SynAmps system the Voltage fields are not relevant for these re cordings assume TTL triggers are coming from the STIM system Step 6 Click on the Epochs label of the display card to set parameters of the recording epoch For this example assume that you want a 100ms pre stimulus interval a 1000ms post stimulus span and ACQUIRE4 2 24 Copyright 2001 Neurosoft Inc Document number 2202 Revision B automatic baseline correction to be enabled during acquisition In the area labeled General set the number of Sweeps to 500 The stimulation device 1 e STIM program should provide at least 500 total events The majority of these events will be used to construct the Frequent waveforms FREQ the remaining events will make up the Infrequent or Rare waveforms RARE Set the Start time for 100 pre stimulus and the end time for 1000ms The auditory P300 evoked response will contain several components peaking both before P200 and after late positive complex 300ms Depending on the task conditions and subject the late components may well extend beyond 300ms and not return fully to baseline for periods up to 800ms post stimulus The 100ms pre stimulus interval will provide a stable baseline that can be used to remove any unwanted offset The post stimulus interval of 1000ms should be of sufficient duration to inclu
129. et value from 100 to Oms and then by subtracting this value from the entire waveform In most P300 recordings we will want to store all of the epoched data to disk and then analyze the data offline with the EDIT module By disabling the Artifact Rejection feature all of the epochs will be stored Click on the Artifact Rejection Enable field if needed so that the check mark does not appear Itis still a good idea to enter the Start 100ms and End 1000ms and the Minimum 50uV and Maximum 50uV settings for later reference enter the values then click to disable In the Display area set the X Start to 100ms the X Stop to 1000ms the Y Min to 100uV and the Y Max to 100uV You will see the entire epoch displayed within the 100uV limits Leave the Y Autoscale OFF no check mark If activated the program will automatically scale the Y scale voltages symmetrically to the greatest absolute value detected during the specified span of the epoch Step 7 For this example we will set the program to perform online sorting and averaging of the RARE and FREQ stimuli Assume that the STIM system has been set to send trigger types codes of 200 for the RARE stimuli and trigger type codes of 100 for the FREQ stimuli Click on the Sorting tab displayed in the Setup window We will specify two averaged files to be sorted and saved We will sort these files ACQUIRE4 2 25 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Criter
130. ettings for the VEOG and HEOG channels Click de SelectAll button so that all electrodes are green In the Amplifier Settings region enter 1000 for the Gain 100 for the Low Pass filter and 0 15 for the High Pass filter ACQUIRE4 2 22 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Amplifier Settings Selected Channel E E Gain hoo d Range 55m Accuracy 0 084 pv LSB Low pass Dn d High pass opt d Apply To All Selected Channels gt Now click the s Bere Ee S button This will apply the above Gain and Filter settings to all chan nels For illustration purposes we ll now change the Gain and Filter settings for the VEOG and HEOG channels Click the DeselectAll button the labels should all be red Double click the VEOG and HEOG channels so they turn green Enter a Gain of 500 and a High Pass setting of 1 0 and click the Apply To All D S SE E E button again You can verify the settings by clicking once on an electrode site and reading the settings in the Amplifier Settings fields When you have entered the settings click OK For non SynAmps systems the Gain and Filter values are set in the Metrabyte or LabMaster option under Edit from the Main Menu bar These settings cannot be changed unless you physically reset the amplifiers enter the new settings on the Hardware display under Edit and recalibrate with those settings Step 4 Now click the Channel Attributes tab As in the p
131. f this manual Select Amplifier AD card Heuroscan Labs Sunamps Neuroscan Labs Nu mp s Neuroscan Labs Quicktrace Eethley Metrabyte DAS 1802 Labmaster 4D Neuroscan Labs Amp Simulator Cancel If you are using SynAmps NuAmps or QuickTrace highlight the appropriate selection and click OK Installation is virtually instantaneous this writes the Acquire ini file in the Windows folder If you are using a Keithley Metrabyte card in the SCAN PC all new systems without SynAmps or NuAmps highlight the appropriate selection and click OK If you have an older system with a Labmaster A D card in the SCAN PC 330kHz card only SCAN 4 2 is not compatible with the 100kHz card highlight that selection The Amp Simulator can be used in place of any amplifiers and it provides a way to simulate acquisition for testing or diagnostic purposes When the SCAN 4 2 AmpInst window reappears click on Done If you wish to change the amplifiers or the A D card run the Uninstall option then run Install with the new selection and click Done when finished To start ACQUIRE you should first double click the SCAN 4 2 icon rAd and the Program Launcher will appear Sp Scan 4 2 s ACQUIRE4 2 10 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Start ACQUIRE by clicking the ACQUIRE icon gt fromthe Program Launcher The main screen will appear Acqume 256 channels alr elm tt Tlie loo lel a 2a Ale ee eles 5
132. file for the P300 recording Click Edit from the Main Edit View Acquisition Options Overall Parameters Individual Channel Settings Channel Assignment Table Make default setup Subject Suramps Hardware Menu bar then click Overall Parameters A multi card window will appear with the Startup card on top For this recording we ll look at the incoming signals from all channels as well as the sorted averages Enable the Multiple Windows Display and the Sorted Average Window Display check mark will appear Make sure no other displays are enabled Set the polarity as desired Negative Up or Down Single Window Display No 1 AA Enable FilterDisplay Derivation Derivation S Single Window Display No 2 E Erable P ete menar P cl ergspen Wenveaton S Multiple Window Display Hien W Enable FilterDisplay Derivation Ue ation S Sorted Average Window Display Spectral Display AN LESSER d wy ence Set Display aa Negative C Up T Display this Wenu on Startup D Auto Save ACQUIRE4 2 21 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Step 3 Now click the Amplifiers tab Set the A to D rate to 5 00Hz SynAmps users toggle to this value Acquistion Amplifier Settings AD Rate 500 Humber of Channels 32 Reset Positions Acquisition Type Epoched AC DC C AC Ge DE DC Correction W Enable E Level Motch T Enable Frequency Se
133. function may also be accessed from the tool bar You should see a decrease in spacings between the second indicators as you decrease the speed Increase Scaling Single and Multiple Window and Frequency displays The Increase Scaling button will increase the scale of the amplitude in the selected screen display ACQUIRE4 2 144 Copyright 2001 Neurosoft Inc Document number 2202 Revision B The gain of the screen will increase by a power of 2 each time you press this key This feature may be selected directly from the tool bar Note that if you are displaying both the Single and Multiple Window Displays you can select either screen by clicking the mouse on the gray horizontal bar at the top of the display It will change to blue or other user selected color when active The scaling options will be applied to the active display The screen amplitude affects only the display and it does not affect values stored to disk Decrease Scaling Single and Multiple Window and Frequency displays The Decrease Scaling button will decrease the scale of the amplitude in the selected screen display This feature may be selected directly from the tool bar Autoscale Single and Multiple Window and Frequency displays The Autoscale function amatically sets the amplitude scaling to the largest and smallest voltages detected from any channel Next Display Page This option allows you to step through electrode display pages that are created when you indi
134. ge such as 10 15 These may be combined in one line 1 3 5 10 12 15 18 20 The averages will be based on these codes Response You may sort on the subject s responses using the response codes sent by the mouse or STIM response pad if you are using STIM or other device for sending a compatible response type code For example if you want to compare responses with the left mouse button to responses with the right mouse button create 2 average files one having a in the Response field and the other having a 2 in the Response field You may specify stimuli using the conventions described in the Stimulus section above Response types from the STIM pad are 1 2 4 and 8 Parameters Artifact Rejection Display and Baseline Correction These areas are the same as in the Epochs section The settings will affect the online averages only Sort file template The Sort file template option allows you to Import and Export the sorting settings as an s7t file When you are satisfied with the settings you have entered click on Export anda Windows Save File utility will appear Enter a file name and click Save to continue To retrieve a saved sort file click on Import and a Window Open File utility will appear Click on the file you wish and then click on Open to continue Audio It is possible to use the audio portion of Windows to enhance data acquisition For ex ample if you are acquiring EMG signals you can hear the EMG amplitude th
135. gram is designed not to allow invalid settings for any of the fields Ifyou click on a field and get an error message saying to input a number within a certain range click OK and see what field has been high lighted It may not be the one you had clicked Enter the appropriate number 1n the field that was high lighted then return to the original field you had selected and continue Note the interaction between the A D rate and the number of points selecting the A D rate in the Amplifier section will affect the sample duration and the number of points in the Epochs section In the Baseline Correction area click Enable and set the Start time to 10 and the End time to 1ms The End point has been set prior to time zero to avoid potential stimulus artifact Baseline correction will be performed online by computing the mean offset value from 10 to 1ms and then by subtracting this value from the entire waveform In the Artifact Rejection area click Enable and set the Start time to 5 and the End time to 100ms The Start and End interval determines the range of the artifact scan In this setup the scan begins after the stimulus artifact Next set the Minimum 50uV and Maximum 50uV voltage threshold settings Values in excess of 50uVs will result in rejection of the sweep Under Display enter 10 and 100 for the Start and End time points respectively if these have not already been set Enter 50 and 50 for the Min and Max microvolt settings
136. he background screen with all of the labeled electrode boxes that you can select one by clicking on the darker bar at the top of the box turns box a highlighted color The label in the Label field will show the highlighted electrode Scalar You can Rename the electrode by overtyping the label You can slect an electrode display eitherby clicking on the display itself on the label bar at the top of the display or by using the up and down arrow buttons at the end of the label field The Scalar feature allows you ACQUIRE4 2 134 Copyright 2001 Neurosoft Inc Document number 2202 Revision B to alter the display scaling factor for individual channels the channel that 1s displayed in the Label field 1s the one that you are setting For example you might want the display scale for the VEOG channel to be different from the other EEG channels In that instance set the VEOG scalar to 0 5 while leaving the other channels with a scalar of 1 The scalar multi plies the global scale factor you have set during acquisition For example 1f the global scale factor is 2 the number displayed on the Status Bar and affected by the Up and Down arrows on the Toolbar and the scalar value is 2 then the display will be multiplied by a factor of 4 The scalar setting affects the screen display only and has no effect on the stored data Size and Position The Size and Position buttons are used to position the electrodes displays automatically
137. he overlay image will be Map Ghost Color This option is used to change the color of the overlay box in the video display Clicking it will display the standard Color display Select a desired color or create you own custom color Set Video Display The graphic interfaces that you see here depend on ACQUIRE4 2 151 Copyright 2001 Neurosoft Inc Document number 2202 Revision B which capture system you are using and its own drivers These are inde pendent of the SCAN software Please refer to your capture system documentation for this information Set Video Source Use this option to make changes to the video camera configuration With the camera display on adjust the controls to the desired settings You will see the effect on the display Turn Automatic Color off to access the grayed out parameters Click the Restore Defaults button to return to the original default settings settings Capture Source Intel USB Video Camera Ill Picture Controls Saturation EH Sharpness E Belboooogoouegoecoeeeoooen dheoopeoegooeoeonaeeoeeeeeeclkeeoeeeeeegeeeeeoeeeeeecl e Brightness Hte Brel let Ale M Automatic Color Exposure Lock Restore Defaults Click the Capture Source tab if you have more than one video device and you want to switch from one to the other Settings Capture Source Set Video Format This option is used to set the video stream settings Click it to see the following d
138. hen click the Start button on the Trigger port test screen The Stimulus value will remain at 0 with only very brief flickers at the time of stimulation Tha Last Stimulus will show the last stimulus trigger type that was received This will be either 1 or 2 for the P300 file The corresponding binary code can bee seen in the Binary equivalent column The Response value will remain at 255 The triggers should show 1 2 4 and 8 when you press buttons 1 2 3 and 4 respectively The Counter should increment with each stimulus The Hold value is generally 0 Click Stop when you are through or Done to exit the testing You may check that the Response pad is working correctly by looking at the triggers in continuous mode Single Window Display in AC QUIRE If you do not receive any triggers or the values are different from the above please call NeuroScan technical support NuAmps Each NuAmps amplifier headbox provides inputs for up to 40 monopolar channels All channels are always recorded as true monopolar channels with the ground as the negative input The reference you select is computed at a later stage Please see the NuAmps manual for more details ACQUIRE4 2 69 Copyright 2001 Neurosoft Inc Document number 2202 Revision B NuAmps setup files Before recording signals from NuAmps you will need to create a setup file or use or modify one ofthe supplied ones recommended Setup files contain all the system configuration inf
139. hether the file was selected for Single or Multiple Window Display Display Page The Display Page feature allows you to assign electrode channels to additional screen display pages To do this click the up arrow button at the end of the Page field The field will display a 1 and the Assign Channels button will become active The screen behind the Channel Layout screen will be empty Click the Assign Channels button and see the Channels in Page 1 display Double click on the individual electrode labels so they turn green to add them to the first additional display page and or use the SelectAll and DeselectAll buttons ACQUIRE4 2 132 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Lbsrert r page 1 Fai Pa Ti per S e Fie W zj ZI C m T on KC GN K i F D e D P Ji om EE GT On Zeck Ai Deko 28 Click OK when you are through and you will see the selected electrode displays Size and position them as desired Through this process you can assign electrodes to any display page you wish You may assign the same channel to more than one display page for example you might want the artifact channel to appear on each display page Use the down arrow button to get back to the original display page Click OK when you are through and be sure to resave your setup file if you wish to retain the changes you have made Note if you have appplied an LDR file to the online display the additional display pages will
140. ia M Enable Online Averaging FREG lt Add Average kg Stimulus 200 IT Response gt gt Del Average Baseline Correction IT Enable Stant mel 100 0 Stopim og Got file Export Import Display P Y Autoscale Start ma mn Stop me Dog Y Min bit Jg YMan pi 100 0 Artifact Rejection IT Enable Stat mel Donn stomme fi000 0 Hii S00 Biar tee enn Start riz Stop mz fi 000 0 Points 551 Avg Update fi using the trigger type codes sent over the parallel I O port from STIM or other similar stimulus presentation system Click the Enable Online Averaging field to access the options if needed Click on the lt lt Add Average button and a Windows Save File utility will appear Enter FREQ and you will see the FREQ name appear in the upper left box on the Sorting screen Under the Criteria section click on the field for Stimulus and enter 100 Set the Parameters Artifact Rejection Display and Baseline Correction fields as you wish These will affect the online sorted averages only The epoched data file that is stored is governed by the settings on the Epochs screen Then click on the lt lt Add Average button enter RARE for the file name enter 200 in the Stimulus field and make any changes to the Parameters Artifact Rejection Display and Baseline Correction fields If you make a mistake and wish to change these files highlight the averaged file you wish to ed
141. ick insode the display once to enlarge it to the intermediary size double click it to go to full size Or you can click and drag a corner of an electrode display to a new size of your choice If you want all of the boxes to be the size of the one you have just set click Make Same Size and all boxes will be set to the size of the one that is highlighted You may also reposition the displays by dragging them from the top label bar on each display Clicking on the Reset Positions button to return the boxes to their original orientation and size Note if you selected more than 32 channels under Amplifiers above and yet only the first 32 are displayed click Reset Positions to see all the channels The positions you set will be reflected 1n the Multiple Windows Displays during acquisition You can for example position the boxes to approximate the 10 20 system as shown below Copyright 2001 Neurosoft Inc Document number 2202 Revision B Small E Hj a mall Small Small F Small Srna SS Small Small EC 5 mall amp mall Small Small Be ma Small Small Small Small P cr Small amal Small Small T H H i S Small T Small Small Small Small Small Small HI i Small When you have successfully concluded the individual channel assignments click on OK to enable the changes Remember to SAVE the Setup file if you plan to use these settings repeatedly Click on CANCEL to exit the individual setting optio
142. ick on the Derivation File button to access a Windows Open File utility Select a linear derivation file dr extension that you created either in the Montage Editor or with a text editor that will allow you to display a bipolar montage as a linear combination of other channels A portion of such a file is shown below In this particular example a 24 channel bipolar montage 1s created from a 32 channel modified 10 20 recording Numbers have been used in place of Electrode channel labels in this example the labels would normally agree with those entered under Channel Assignments Note if you plan to do online mapping of the LDR data file the labels in both the map and dr files must agree exactly including case 2432 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 25 2 4 OO 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 00o 0a 00 DUDU UU 3 5 OO 0 0 1 0 00 10 00 00 0 0 0 0 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 5 OO 0 0 0 0 1 0 00 0 0 0 0 1 0 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 00 0 0 0 0 0 0 5 9 OO 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 00 00 0 0 0 0 UU ol 00 0 0 0 0 0 0 0 0 OO 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 00 0 0 0 0 0 0 4 15 00 0 0 0 0 0 0 0 0 OO 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 12 16 00 OO 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 13 17 00 00 OO 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
143. iles when have completed the settings Then click OK to apply the current changes you have made if you wish to begin immediate acquisition Click ing CANCEL will exit the Edit section without saving any changes you have made Channel Layout Selecting this option brings up the Channel Layout dialogue box as well as the individual electrode displays Ifyou retrieved an existing setup file such as QCap32 ast the displays will appear in a layout that approximates the cap placements If you created the setup file using the Make Default Setup option the displays will appear in a matrix form The following features are seen in the Channel Layout display ACQUIRE4 2 131 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Channel Layout Electrode Array Original array K Display Page Page jo Heston WHehhels Single Window Order Size and Position 10 20 Positions Reset Positions Make Same Size Import Positions Label foz Export Positions Scalar I Adjust Positions OF Cancel Electrode Array This feature allows you to select the Original array or up to 3 arrays created with dr files assuming these have already been selected on the Startup page under Overall Parameters under Edit from the Main Menu bar By highlighting an dr array from the pull down list you may alter the size and positions its electrode channel windows The label in the array window will designate w
144. ill allow access to additional windows with various configura tion features and may be selected by clicking on the tab portion of the index card where the label is displayed Single Window Display No 1 W Enable FilterDisplay Derivation Derivation File Single Window Display No 2 E Enable Eiter Displau Derivation Uerivation File Multiple Window Display foe SS Ea ee If Enable Filter Display D Derivation Ueryation S Sorted Average Window Display Spectral Display An IT Enable d IT Enable Negative C Up Down T Display this Menu on Startup D Auto Save Two methods are employed for changing acquisition values depending on the type of the variable Some variables known as toggles describe different states of the system These variables have a limited number of states such as ON and OFF To change the state of these variables place the pointer on the variable and press the left mouse button or click on the down arrow to see a pull down menu Look through the available options and select the desired state The other type of variable is a continuous variable To change these variables click the pointer on the variable field and type the desired value from the keyboard In the listing below state variables are designated as toggle and continuous variables are designated as keyboard Set Weoley Eiter ACQUIRE4 2 79 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Startup ref
145. ill see the entire epoch displayed within the 20uV limits Leave the Y Autoscale OFF no check mark ACQUIRE4 2 44 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Click on Fp Average on the display card to access the settings for an F recording Select Both for Display F Max g Terminate Method Parameters C None single Point Pos ms F as Window Start ms Noise Max pi 0 2 Noise Level Window Stop riz fe Both F Value Term Channel s Im Sweeps per Block zoo om op Noise Level pi Selected the Terminate Method set the Terminate Channels to All the Single Point Position to 5ms the Window Start point to 2ms the Window Stop point to 10ms the Sweeps per Block to 200 the F value to 3 1 and the Noise level to 0 02 Under the Display area enter 20 for the F Max value and 0 2 for the Noise Max value These settings will configure the system to compute the F over a 2 to 10ms interval The single point will be located within the window at Sms A yellow line located at 3 1 will be shown in the F display indicating the F Value threshold value Termination will occur automatically if either the F value 3 1 or the Noise Level 0 02 criterion is reached otherwise termination will occur after 20 000 sweeps or by clicking on the Stop button on the Toolbar Step amp This is an optional step that is included for comparison s sake To show the advantage of
146. in Otherwise the new position information will be lost For this tutorial let s create a second display page that will focus on the posterior leads where the VEP will be most prominent Click the up arrow in the Display Page area so that a 1 appears Then click the Assign ACQUIRE4 2 17 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Display Page Page fi Assign Channels Channels button Double click the PZ O1 OZ and O2 electrodes so that they appear in green while the others are still red and click OK The 4 channel displays will appear Resize and reposition these as desired The result may look similar to the following figure Channel Settings II x Click OK to continue and be sure to Save the setup file again if you wish to retain the new display page File Edit View Ac use Load setup from the Main Menu bar During acquisition you may click on the Next Display button 121 on the Toolbar to see the second display page Step 10 Now go back to the Edit option from the Main Menu bar and select the Channel Assignment Table This displays a list of the physical channels and the labels created for each You can reorder the physical channels if desired please refer to the SynAmps manual for more details You can also use the display to relabel channels If you have more than 32 channels there will be additional tabs at the top to view the additional channels ACQUIRE4 2
147. in the Operating ACQUIRE section below Under Scaling select the Amplitude option by clicking on the adjacent field The amplitude option takes the square root of the power spectrum to express the units in microvolts same as DOS version Magnitude Under Acquisition Mode select Ensemble Average by clicking on the adjacent field Ensemble averaging is more fully ex plained in the Operating ACQUIRE section below but is analogous to averaging evoked responses with updated sweeps Note that when you select the Ensemble option the Sweeps field and the option to Save become active Enter 5 for sweeps and enable Save to store the online frequency data You will be asked for aname and path designation for this file as you begin to acquire the data You might elect instead to use the Running Average option Select a Smoothing factor such as 3 and the online maps should appear smoother Under the Display area click on the Histogram field to have the FFT results displayed in bar form X Start and X Stop set the range for the display of the computed frequencies For this recording enter 0 and 50 respectively to have the power in the 0 50Hz range displayed Leave Y Min and Y Max blank or leave any numbers that are present and click on the Y Autoscale field This will automatically scale the microvolt amplitude according to the maximum value encountered in the epoch In the Window area select 512 for the number of points Remember these settings are fo
148. in the case of changes in wakefulness For example if the power spectrum is computed on a single sweep the variability of the estimate will be equal to the estimate itself Variability decreases proportionally to the number of sweeps in the average The more sweeps the better the estimate of spectral composition If your EEG is relatively stable over a series of discrete epochs a valid method to estimate EEG frequency content is to average in the frequency domain When you click Ensemble Average note that 1 the Sweeps field becomes active and 2 you will have the option to Save the results as an AVG file Enter the number of sweeps to be used in the ensemble average in the Sweep field and enable or disable the Save field as desired The Running Average when enabled allows you to perform an online smoothing operation with the FFT and online maps ACQUIRE waits until the specified number of Spectra have been collected the number in the Smoothing window then it will sum the sweeps and then average them When a new sample sweep comes in 1t replaces the oldest sample and then reaverages Itis essentially a running circular buffer or a moving spectral average The Running Average is applied to online FFT spectra and maps The Running Average affects the display only and has no effect on the saved data Display The display option area allows you to specify various parameters of the online EEG spectrum display You may first select whether y
149. indow display or Averaged display With the Single Window Display the channel nearest the position of the mouse indicator will be shown The Status Bar fields will then show the Display Page number the Scaling factor the Electrode label the time in milliseconds and the microvolt voltage corresponding to the mouse position Ifthe Spectral Data option has been selected in the Start Acquisition dialog box and the mouse is positioned in one of these electrode fields you will see the corresponding Frequency Hz and uV values in the Status Bar displays Batch Immediate Many of the basic operations in ACQUIRE can be controlled by BATCH commands Please refer to the Tcl Batch Command manual where these are described in detail Waveboard The Waveboard may be activated from this line or more easily from the icon on the Toolbar E The Waveboard allows you to perform a variety of operations including marking amplitude and latency points measuring amplitude and latency differences between two points overlay wave forms combine waveforms from different files and so forth Itis explained in detail in the Waveboard appendix attached to the EDIT manual Briefly you may copy waveforms from any Multiple Window Display to the Waveboard by clicking the right mouse button within an electrode display for the option to send a channel ACQUIRE4 2 155 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Video This option requires
150. ions appear and instead you see the Amplifier Simulator line then you need to run the AMPINSTALL program If the appropriate option is present click on it to enter review or edit the settings Realize that making changes on the Metrabyte and LabMaster screens necessitate corresponding changes on your amplifiers and in most cases will require recalibration before collecting additional data Acquire 64 channels File Edt View Acquisition Options Window Help 1 Acquisition is controlled by commands listed in the pull down menu under View or more easily from the tool bar located just beneath the Main menu bar The tool bar is enabled by clicking on the Toolbar field in the View menu check will appear The commands may be accessed with the mouse during acquisition The following is a description of the actions of each command ACQUIRE4 2 143 Copyright 2001 Neurosoft Inc Document number 2202 Revision B View Acquisition Optons Window Help Accelerate Display Key Decelerate Display Kep Increase Scaling Up Arrow Decrease Scaling Down Arrow Autoscale Nest Display Page Page Up Previous Display Page Page Down Baseline Correction Display Only Ctrl B vw Invert Polarity Display Only Ctrl l Perform DC Correction ROESER vw Toolbar w Status Bar w Batch vw Immediate Waveboard Video Accelerate Display single window display only The Accelerate Display option will increase the speed of the display fewer
151. is the intersection ofall three axes in the center of the head The Polar positioning system uses Phi Theta and Radius values to express the location ofthe electrodes as follows The center of the display above is 0 0 0 As you move away inany direction from the center of the display Theta becomes larger 90 at the outer ring The Phi and Theta values can be read in the lower part of the display Curent Pos Phi 1 9 Th 1 153 Phiiszeroall along the line from the center point to the right ear As you move the cursor up and in a counter clockwise direction Phi will increase up to 180 the line from the center point to the left ear Crossing that line Phi becomes negative and increases from 180 to 0 as you continue ina clockwise direction below the x axis The Radius is the vector length from zero to the Phi Theta location thus pin pointing the electrode position in three dimensions Copyright 2001 Neurosoft Inc Document number 2202 Revision B The same electrode position can therefore be expressed in X Y and Z coordinates orin Phi Theta and Radius vaues All six values fora given electrode are shown in the table on the right side of the display FZ adat aff 128 13 0 30 rodo 101 27 III 138 92 0 00 16 00 138 00 56 94 2235 13291 270 5047 12293 Double click on one ofthe electrodes to see the following display Electrode Coordinates Label JPZ Phi UL PAS Theta 141 0136 Radius 1132 911 A 1 17568
152. isition that contain voltages in excess of criteria that you set The Start and End points specify where in the epoch a scan for artifact will begin Ifa value Artifact Rejection M Enable Start me UU End rng fi 000 Mm p Let Man p en exceeds the Minimum or Maximum in the interval on a selected channel the sweep is rejected Otherwise it is accepted ACQUIRE4 2 114 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Enable Turn this toggle ON check mark will appear if you desire artifact rejection Start ms The Start point sets the position within the sampling period in milliseconds at which the search for artifact will begin End ms The End point sets the position within the sampling period in milliseconds at which the search for artifact will terminate Min uV The Minimum value is the low cutoff value for artifact rejection Values lower than the artifact Minimum will result in rejection of the sweep Max uV The Maximum value is the high cutoff value for artifact rejec tion Values higher than the artifact Maximum will result in rejection of the sweep Note Be sure to enable at least one of the channels for artifact rejection see Artifact Rejection in the Individual Channel Settings section below Display These fields allow you to set the millisecond and microvolt limits for the display waveforms Fsp Average Display Min me 1 100 A Mag ms f O00 Y
153. isplay ACQUIRE4 2 152 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Stream Settings Digital Image Format Resolution Pixel Depth bits and Compression Size bytes e40 s 450 420 460800 Change the resolution and Pixel Depth as desired changes to the file size will be displayed Video Snapshot and Video Snapshot with Overlay These options provide the same snapshot taking functions as the icons described above Bitmap files are created with and without the overlay Pause Resume This is the same as the Pause Resume icon described above pauses and resumes video Snapshot Path Use the Snapshot Path option to set the folder in which you want the bitmaps to be placed Use the Browse button to select a different folder if desired The Root Name is the first part of the file name that will be used In the example below headmap was used as the root Successive snapshots will be automatically labeled as headmap1 bmp headmap2 bmp headmap3 bmp etc Snapshot Path Snapshot Jess Gand Browse gt gt Root Name headmap The one remaining option related to video not found on the Toolbar or on the right mouse button options is found under Options Video Select Capture Device Select Capture Device If you have more than one Capture Device this option is used to select the one to be used Select ideo Capture Device Microsoft WOM Image Capture Version 4 10 0 2222 sl Cance
154. isplay Automatically sets the amplitude scaling to the largest and smallest voltages detected from any channel Decelerate display Single Display only Slows the speed of the signal display If you have multiple display windows open only the one that is highlighted will be affected Accelerate display RS Single Display only Increases the speed of the signal display If you have multiple display windows open only the one that is highlighted will be affected Show next display page H Displays next page of selected electrodes will not be highlighted unless additional pages exist If you have multiple display windows open only the one that is highlighted will be affected Show previous display page Elt Shows previous page of selected electrodes will not be highlighted unless additional pages exist If you have multiple display windows open only the one that 1s highlighted will be affected Baseline correction m Single and Multiple display Centers display of signals with labels on the left of the screen display only Ifyou have multiple display windows open only the one that is highlighted will be affected Perform D C Correction DI SynAmps only Performs single DC correction with each press to remove DC components will center signals at the baseline See the descrip tion above for some important considerations when using DC corrections Invert polarity Single and Multiple display Negative voltage upwa
155. it and make the changes If you want to delete an average file click on the gt gt Del Average button After you have entered the settings you wish click on the Export button under Sort file and a Windows Save file utility will appear Enter a file name the srt extension is added automatically and click Save To retrieve a saved file click on the mport button and select the file with the Open File utility Step amp Click the Misc tab We will set the parameters for the Ocular Artifact Reduction transform here and they will be retained in all data files recorded with this setup file See the EDIT manual for an explana tion of the settings for now just enter the values as shown ACQUIRE4 2 26 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Ocular artifact reduction Trigger av a ae f Positive C Negative Threshold 10 00 Blink values Min sweeps E Ee Duration ms 1400 EOG The Fsp Average Online Frequency Analysis Audio Mapping and Events sections are not involved in these recordings Step 9 Click on Save As and label the Setup file P3O00SET the ast extension will automatically be added Click Save then click on OK Make any changes to the electrode labels or the electrode positions using the Channel Assignment Table and the Channel Layout display as desribed in the previous tutorial Step 10 To begin viewing click Acquisition on the Main menu bar followed by
156. ition Termination will occur after 500 sweeps are accepted or after user interrupt by clicking on the Stop icon Single sweep recording and an online average SEP This section describes how to configure ACQUIRE for simultaneous online averaging and storage of single sweep epochs to disk This option is particularly useful for monitoring both the acquisition of the average and the reconstruction of the average offline with the EDIT module Artifactual sweeps that may not have been removed in the online average can be removed or corrected offline to minimize their effects on the reconstructed average A configuration for a somatosensory evoked potential to median nerve stimulation will be given to illustrate simultaneous average and epoch based acquisition Follow these steps to configure the system for online SEP collection Step I Start ACQUIRE as described above and you will see the ACQUIRE main screen ACQUIRE4 2 28 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Arouge 256 channels ln lols lA le we elm ye Bt oe Rig S A fe Se ee GE Step 2 Since we will be using only a few channels for the SEP recording in this example begin by selecting the Make Default Setup under Edit and say OK to the Warning Make default setup This restores all the default settings 1f you have made any changes to an open setup file be sure to Save these before using the Make Default Setup option
157. ition or in offline editing the Skipped channel labels will be in black type regardless of the color you had selected for Text under Options Bad This option allows you to select certain electrodes to be excluded from certain statistical operations such as averaging variance computation mapping or artifact removal check mark will appear when toggled on Select the Bad channels as described above Bad channels in contrast to Skip channels are encountered during acquisition resulting from bad electrodes or abnormally high artifact When viewed in acquisition or in offline editing the Bad channel labels will be in red type regardless of the color you had selected for Text under Options Note As a general rule Bad and Skipped channels will not be in cluded in operations that involve the combination of multiple channels These include for example Autoscaling Spatial SVD Common Average Reference and Global Field Power Mapping Ocular Artifact Reduction etc and the channels marked as Bad or Skip will be ex ACQUIRE4 2 107 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 108 Copyright 2001 Neurosoft Inc Document number 2202 Revision B cluded from these operations On the other hand channel by channel operations such as filtering are performed on Bad and Skipped channels as well That is if the operation performed on the Bad channel does not affect the same operation performe
158. izontal dashed lines ACQUIRE4 2 62 Copyright 2001 Neurosoft Inc Document number 2202 Revision B rel Na CG Hz DAD Im w00 agi Ti T zea 1500 01 GU op TO C TTT op ap on op mn Sp Do Sp ao aen a You can move the lines by grabbing them with the left mouse button and dragging them to a new position If you position the mouse between the two vertical lines and above the upper horizontal line you may drag both vertical lines at the same time thereby preserving the frequency interval between them With a power spectrum display the lower horizontal line must remain at zero The two vertical lines define the frequency band width that will be mapped These setting override the ones you made on the Mapping display under Overall Parameters By clicking one of the active buttons on the Active Map display you can direct the frequency band defined by the two vertical lines to the map display of you choice For example if you set the vertical lines as shown above and click the Alpha button on the Active Map display the Alpha map will display that frequency range The frequencies shown below the map will change accordingly and Alpha will appear to the side of the Spectral Data title letting you know which map has the focus for the selected frequency range By moving the horizontal line up and down grab and drag with the left mouse button you can vary the scale on the map the
159. izontal line must remain at zero The two vertical lines define the frequency band width that will be mapped These settings override the ones you made on the Mapping display described above By clicking one of the active buttons on the Active Map display you can direct the frequency band defined by the two vertical lines to the map display of you choice For example if you set the vertical lines as shown above and click the Alpha button on the Active Map display the Alpha map will display that frequency range The frequencies shown below the map will change accordingly and Alpha will appear to the side of the Spectral Data title letting you know which map has the focus for the selected frequency range By moving the horizontal line up and down grab and drag with the left mouse button you can vary the scale on the map the has the focus for the new frequency range Time domain file mapping If you selected Average under Data Source in the Mapping option screen the map will be linked to the average data file a time domain file You should be saving the data to see this function The operation 1s basically the same as for Frequency domain mapping The difference is that you will be mapping the average of the data points within the window you set An enlarged display of one of the channels in the online average might appear similar to the following ACQUIRE4 2 126 Copyright 2001 Neurosoft Inc Document number 2202 Revisio
160. k Save Workspace Use the standard Save As utility to enter a file name and path the aws extension 1s added automatically Itis a good idea to make a backup copy of your workspace files in the event one you use often becomes corrupted Video The Video option is used to setup up parameters for the video camera and for taking snapshot pictures These options are more easily accessed from the icons on the Toolbar Ki KE Ka amp amp or from the right mouse button options found by clicking in the video display screen These are all described above in the Toolbar icon section Batch This option accesses the Autorun feature used with BATCH files Please refer to the Tcl Batch Commands manual for operational details Acquire 64 channels File Edt View Acquisition Options Window Help 1 Window These options function as with any typical Windows program and serve to organize the Bis Options He Cascade Tile Horizontally Tile Vertically presentation of multiple windows on the screen Cascade Selecting Cascade aligns the windows in a stack of overlying cards with the edges exposed to allow access for highlighting as shown below ACQUIRE4 2 175 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Muhlen AA Perea Sara t Ji Gi Ka d IC en Leed KE OT r AEN at aCe a eke bir d i EE ba AR eren ly RAN EN d Tile Horizontally Tile horizontally will automatically arrange windows in
161. l Click the pull down arrow to see and select the desired device ACQUIRE4 2 153 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Launch Waveboard 71 Clicking on this button launches the Waveboard The Waveboard is useful for measuring amplitudes and latencies at specified points on the waveforms Waveforms are selected and sent to the Waveboard where the measurements are made The complete operation of the Waveboard is described in the Waveboard Appendix at the end of the EDIT manual Launch Montage Editor 2 This icon may be used to launch the Montage Editor The Montage Editor may be used to create of modify a montage or linear derivation file without leaving ACQUIRE Refer to the Montage Editor Appendix at the end of the EDIT manual for complete details SOURCE 3 This icon will be active if you have installed the SOURCE software SOURCE 1s an additional plugin program that uses a variety source localization models to compute single or multiple dipole sources It can be used online in ACQUIRE or offline in EDIT Please refer to the SOURCE manual for operating instructions If you are interested in finding out more about SOURCE please see our website www neuro com or contact sales neuro com SEP Stimulator Control The SEP stimulator control is seen only if you have the stimulator connected The SEP stimulator is enabled disabled with this icon Please refer to the SEP Stimulator manu
162. l allow triggers to be registered and sweeps to be initiated on the basis of a user selected voltage on a selected channel Whether the option is available or not depends on which acquisition you have and which acquisition mode you are using For example voltage triggering 1s not supported at all with NuAmps It is available for SynAmps as long as you are in Epoched acquisition mode The best thing to do is to install your acquisition system Copyright 2001 Neurosoft Inc Document number 2202 Revision B using ampinstall exe and then see if the Voltage fields are grayed out If they are grayed out try Epoched acquisition mode Ifthe option is available these fields will become active f Yoltage Channel Lupp e Threshold p li g Slope Ge Pos Nea Bechto Channel Select the channel that will carry the voltage trigger signal Threshold Enter the threshold value in microvolts Slope Indicate whether the voltage trigger will be in the positive or negative direction You can also rectify the channel which will cause all of the voltages to have the same valence In the example shown a sweep will be initiated when a positive voltage from the VEOG channel exceeds 100 uVs Epochs The features in these fields allow you to specify parameters of the recording epoch Click on the tab portion of the display where Epochs 1s shown to access General Baseline Correction Artifact Rejection and Display values
163. l amplifier Gain value is entered in the Setup screen for the AD card under Gain under Edit from the Main Menu bar Ke Metrabyte DA 1900 Setup Metrabyte DA 1900 Setup 1800 Setup Amplifier Dam 20000 Low pass Hz E High pass Hz p E T Top Out Geschl Base This value needs to be as close as possible to the actual gain of your amplifiers You should consult your amplifier manual or vendor to make sure of the settings or use an oscilloscope to verify that the output is in the 1 V range by trying different amplifier gain settings If you are still getting incorrect calibration values in the ACQUIRE calibration routine 1 e something other than approxi mately 1 s as described below then you may need to adjust the Gain setting entered in the AC QUIRE AD card settings Deviations can occur as a result of imprecise attenuation or amplification settings With some amplifiers it may not be possible to determine the exact output voltage of the amplifiers using the settings on the amplifiers front panels or even from the vendors If you are not sure of the actual amplification gain use a mid range setting on your amplifiers and adjust the Gain in ACQUIRE to get 1 s for Calibration values Ifthe calibration values are incorrect and you are unsure what the Gain value should be try the following Calibrate using a Gain of 10000 Divide the Gain value you have entered by the calibration values you obtained For example if the Gain
164. l device typically the STIM system Click on the Triggers label The Hold value should be set to 0 see the triggering section in the Operating ACQUIRE section below for special circumstances Startup Amplifiers Channel Attributes Triggers Ge External Hold value lo IT Use inverted values nemal literal e oz Creating Click Stimuli in STIM Ifyou are using the Sound module in the STIM system to provide clicks for the ABR we recommend the following settings For the clicks themselves use a delay of zero a duration of lms and 100 of maximum amplitude these settings are available when you create the click under Transforms Generally you record the clicks from one ear then the other with masking in the contralateral ear Also the decibel level is typically varied to obtain the best ABRs similarly the masking volume may also be adjusted as needed Rarefaction is indicated by an upward ACQUIRE4 2 42 Copyright 2001 Neurosoft Inc Document number 2202 Revision B deflection for the click in the main display window in Sound To get Compression go under Transforms and rescale the values with a 1 This will be seen as a downward deflection of the click Under the Config section in Sound try the following settings at least initially then vary them as needed In most situations these settings are optimal for obtaining the best ABRs however they are offered as sugges tions not as an absolute guideline Not
165. lected Channel i Gain 1000 7 Range 5 5 mi Accuracy U 064 vu JP Low pass 100 Hz High pass 0 15Hz Apple To All Selected Channels non SynAmps users enter the value from the keyboard if it is not set already The digitization rate of 500Hz will provide enough resolution to determine peak latency and amplitude of the major components Next enter 32 for the number of channels if not already set For Acquisition type select Epoched This setting configures ACQUIRE to collect only epochs of EEG sampled around the stimulus event trigger and save them to disk Real time display of the individual sweeps 1s shown as they are acquired n general we recommend that P300 and similar type recordings be acquired using the Continuous Acquisition type the Epoched example is presented primarily for illustration purposes The advantages and disadvantages of continuous mode acquisition are described in the Continuous recording tutorial using the N400 paradigm If you are using SynAmps under AC DC select DC Correction with a 70 correction value enable the Enable field If you are not using SynAmps this section will not be accessible In this example we will enter the Gain and High Pass filter settings for the VEOG and HEOG channels independently from the EEG channels SynAmps only Note Ifyou intend to use the Ocular Artifact Reduction in EDIT to subtract eye blink artifact we recommend that you use the same Gain and Filter s
166. lectrode label in the Selected Channel Then click inside the montage display area not on a label to see the change Properties Selected Channel El FF1 E Physical Channel Calibration Impedance Physical Channel The Physical Channel field displays the number of the actual amplifier channel that is carrying the signal that is displayed in the Selected Channel field If you have a SynAmps you may remap the Physical Channel that is you may change the physical channel that is being used with the Selected Channel You cannot remap non SynAmps systems Calibration The Calibration will display the Selected Channel s calibration value The default calibration value for all channels is 1 After you perform a Calibration and save the calibration values with the setup file these values will be seen in the Calibration field Impedance The Impedance field shows the values that were observed when you exited the Impedance routine These values will be stored along with the data file and may be reviewed channel by channel in EDIT ACQUIRE4 2 106 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Artifact Rejection Clicking on the Artifact Rejection MR Ajtifact Rejection field allows you to designate one or more channels that will be scanned for artifact in the automatic artifact rejection of sweeps Typically electrodes that monitor eye movement as well as those that pick up other sources of artif
167. ll be active if you selected the Band Stop type of filtering above Enter or select the desired options as described in the Band Pass section above Note Band Stop filtering is basically the opposite of Band Pass filtering Band Pass filtering affects frequen cies primarily outside the designated range and Band Stop affects frequencies primarily within the designated range Click OK when you are ready to continue The incoming display waveforms will be filtered accordingly This option has no effect on the data that are saved only the filter parameters you set via the SynAmps software or the actual settings on your amplifiers will affect the data that are saved The option allows you to observe incoming data that have for example attenuated slow wave artifacts or attenuated EMG You might for example have the Single Window Display No 1 show the essentially raw incoming data and have the Single Window Display No 2 show the filtered data It is also useful for software programs outside of SCAN that tap into the online filtered data Derivation Linear derivation transforms allow you to create new channels as arbitrary linear combinations of existing channels For example you might create a linear derivation file that recom putes the incoming unipolar montage as a bipolar montage and display this with one of the Single Window Displays Adjacent to the Filter Display options are the Derivation fields Ifyou enable the Derivation option you shoul
168. lues are stored as part of the setup file If you acquire data using more than one setup file you will need to complete the calibration process for each one This is true for the Metrabyte LabMaster and SynAmps systems If you make any changes to the gain or filter settings on your amplifiers you should recalibrate the setup files You should periodically rerun the calibra tion process for all setup files to verify that the amplifiers are stable and functioning properly How often should you calibrate There 1s no absolute answer to this question Solid state amplifiers should not vary significantly over periods of months or longer However the calibration procedure provides a quick test of the system to make sure all amplifiers are functioning properly A quick daily check is not necessarily too often 1f your particular method is not overly involved and it is a nice reassurance to include in a publication Monthly checks could result in significant data loss ifa channel malfunctions shortly after itis checked There is no harm in calibrating frequently there is risk in calibrating too infrequently The calibration values become part of the header of acquired data files and may be reviewed and edited in EDIT ACQUIRE4 2 163 Copyright 2001 Neurosoft Inc Document number 2202 Revision B If all of the calibration values are consistently too high or too low and you are certain that your calibration settings are accurate then you may need t
169. may Startup Amplifiers Channel Attributes Triggers Epochs Fsp Average Frequency Sorting Du W Enable Online Frequency Analysis Scaling f Amplitude Acquisition Mode Display Ge Ensemble Average f Histogram Sweeps 2 M Save Individual Sweep Power f Line Update f Start Hz fo stop Hz 40 Y Min Bunning Average smaatima pe VF indian Points Al Resolution 0 576563 Length 10 Range G t Max IT Y Autoscale Duration Type Cosine Wea select a variety of settings for the calculation and display of the online FFT Scaling The first area asks which of 2 options you would like for computation of the EEG power spectrum amplitude and power The amplitude option takes the square root of the power spectrum to express the units in microvolts Same as DOS version Magnitude The power option computes a standard power spectrum with values expressed in microvolts squared Window Windowing is employed to control spectral leakage The first choice is the number of Points used in the analyses which must be a factor of two Selecta setting by clicking on the pull down arrow to the right Note that the number of points you select is directly related to the frequency Resolution The greater the number of points the more precise the resolution A good compromise is 512 points yielding a frequency resolution of approximately 2Hz The Range indicates the frequency
170. more SynAmps Discrete epochs are windowed around trigger events with pre trigger intervals supported Trigger events can be generated internally 1 e ACQUIRE triggers an external stimulation device LabMaster and Metrabyte systems only or externally i e an isolated external device such as the STIM computer triggers ACQUIRE all A D systems An external trigger pulse may arrive via a parallel port or an analog to digital channel ACQUIRE can save online average files single sweep and continuous EEG files that can be subsequently processed offline using the EDIT module ACQUIRE has several online analysis and display features including D frequency analyses linear derivation calculations digital bandpass filtering topographic EEG potential mapping stimulus and response sorting with variable length windowing audio monitoring of one or more recorded channels video recording capabilities direct somatosensory stimulator control online dipole source computation requires the SOURCE program ACQUIRE records and displays continuous EEG data along with a stream of stimulus and response event markers This continuous recording can then be broken down into discrete epochs with user selected pre and post stimulus intervals The general steps to collect data with ACQUIRE are 1 create or select a setup file to configure the system 2 enter subject information 3 acquire and save the data ACQUI
171. n B 559 63 ms 15 63 ms Re z 2720 8 2250 0 1500 0 r500 ZOU D 1500 0 p 0 0 E ee EES ger ae L 2250 0 DO T TI IT 0 0 ZOU D 200 D 750 0 1000 0 In this example we used a signal generator to send a sine wave to all channels The map will display the average of the points within the span of the vertical lines As above you can direct the new mapping parameters to any of the online maps by clicking one of the buttons on the Active Map display Active Map H E H H Weta Alpha Bete Beta The horizontal lines are used to set the scaling values for the map Set the red and blue lines to the upper and lower limits of the scale and the green line will be positioned automatically at the midpoint between them In the above example the map was all white zero voltages as the positive and negative values averaged each other to zero Right mouse buttons with online mapping If you click the right mouse button on an online map you will see the following list of options Scale min mas values Color Scale Type Load MAF file vw Show electrodes Shot electrode trata Use Linear Method These perform the same operations as described in EDIT for offline mapping ACQUIRE4 2 127 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 128 Copyright 2001 Neurosoft Inc Document number 2202
172. ncounter difficulties during acquisition using the 5000 Hz AD rate reduce it to a slower speed The maximum AD rate for NuAmps is 1000 Hz The rate will determine the duration of the sampleaccording to the following ratio Total Duration secs of points Digitization rate For example if the number of points is 500 and the digitization rate is 500 times per second or 500Hz then the duration of the sample epoch will be 1000ms or 1 second There is an interdependence among the following parameters number of points per sweep described below digitization rate pre trigger interval X minimum described below and post trigger interval X maximum described below If you change any one of these parameters another parameter will automatically be changed along with it in order to maintain a consistent relationship among all four as follows E Ifyou change the number of points per sweep a new sample interval will be created by increasing decreasing the post stimulus interval X maximum E Ifyou change the digitization rate a new sample interval will be created by increasing decreasing the post stimulus interval X maximum E Ifyou change the pre trigger interval X minimum then anew number of points will be computed E Ifyou change the post trigger interval X maximim then a new number of points will be computed Note the interval between sampled points is the reciprocal of the digitization rate Number of Channels N
173. nd Wahlgreen 1985 Click on the tab portion of the display A dialogue box will appear that allows you to set the parameters relevant to the F average mode The Fsp screen will only be accessible when you select P Average under Acquisition Type on the Amplifiers section Terminate Method Parameters Display C None Single Point Pos rvs F Max 20 000 EValue Window Start ma Noise Max ban 0 300 Noise Level Window Stop ms f Both Sweeps per Block F Value Term Channels Noise Level p fe Al Selected Listed below is an explanation of each of these parameters Terminate Method Select one of the options below 1f you want to employ an Fp termination method ACQUIRE4 2 116 Copyright 2001 Neurosoft Inc Document number 2202 Revision B None Data collection will continue until the specified number of sweeps has been collected F value It is possible to terminate data acquisition when the current F value equals or exceeds a specified F value The F value field sets the terminate criteria A typical value for this field for the ABR is 3 1 The F criterion will terminate acquisition when the terminate method is set to F Value or BOTH on the designated channels Noise level Data acquisition can also be terminated if the background noise 1s lower than a specified value The noise value field sets the terminate criteria A typical value for this field is 0 02uc The noise criterion will
174. ndow you will see the average waveform develop with updates as specified in the Epochs section under Edit Overall Parameters Refer to the description of the Options section below in the Operating AC QUIRE part of the manual to see how to change display colors and to select different aspects of the win dow display If you did not select Auto Save click the Record icon the red dot on the Toolbar P U when youare ready to begin recording The adjacent button with two vertical lines is the Pause button and the button with the black rectangle is the Stop button The figure below displays the results of an online pattern reversal VEP In this example we also enabled a single window display to monitor the subject s EEG more easily during the recording ACQUIRE4 2 19 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Erpes Pit pharrr l kb elegy rn Ele Ech i p setze Hab FIn WR Di ri er D P Geste E rere ne E F d s M e Bee i l i mje m y bk H pE e enn mas mm zm remm e wem s a mg eg Ke egen nt ee Wi E ee ee ee A E EE E Bi WA Ke W Ni Bau Dg ne Ae a ein a el Val m a zm bis a aa ai ee ie Gl ich bb i oy tb labo iy e on ee gees oe ell cmt i oF Rael getest Angel eut np as ei A m BAR ten ee Fria Adah Ya fer ge meee mse Be IR pm aye me aa e em s Mech sae a EPS i EE SNE GEE a En j E a gaa am pa E E a a aeadh oa ee
175. ng EEG with attenuation of the anticipated increase in EOG activity due to the task Follow the steps below to configure the system Step I Start ACQUIRE as described above and you will see the ACQUIRE main screen Acqua 256 channels ell E ee E SE te EES PAPE SE OESSI EET NEE ST OSOEE TEES E P im TA L h S Oe vt e Dr Pal en i KK KR E i E CN g For De puert FI m O ees oo amp Step 2 The next step is to create a Setup file for continuous recordings The easiest way is to start with the QCap32 ast setup file and modify it We ll omit the HEOG channel for this recording ACQUIRE4 2 47 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Step 3 Click Edit from the Main menu bar then click Overall Parameters Edit View Acquisition Options Overall Parameters Individual Channel Settings Channel Assignment Table Make default setup Subject Sunamps Hardware A multi card window will appear with the Startup display on top For this recording Enable the Single Window Display No 1 check mark will appear For demonstration s sake let s say also that you are recording with your filters set wide open However because of likely increased EOG activity inherent in the task you also would like to see in real time how the data would appear with additional filtering To do this click on the Enable field for Single Window Display No 2 Now click on the Filter
176. nitially clicked the Type annotation button You must be saving the data for this option to be active Video capabilities in ACQUIRE Ifyou have a PC camera installed on your computer you may use it in ACQUIRE The primary function of the camera during acquisition is to map your online data on to the picture from the camera One common example is the case where you have an electrode grid lying on the surface of the brain and you want to map the EEG EP activity to the grid while recording the data This would allow you to visualize for example the spread of epileptic activity Video controls Ki Ka Ka es amp These icons are used if you have a video camera connected to your PC It will have its own installation software Make sure the camera is working before you try to use it in ACQUIRE ACQUIRE4 2 148 Copyright 2001 Neurosoft Inc Document number 2202 Revision B View Video i If the camera has not been started in ACQUIRE only the first of the 5 icons will be functioning Click it to enable the camera display The remaining icons will then become active The demonstration below will simply map EEG results to a dummy head This is to illustrate how the process works The display will appear with a green box yours may be a color other than green fee Real Time Video Overlay The box is the overlay and it is used to superimpose a map display on top of the image that you are seeing through the camera It can be resi
177. note you wish When you replay the continuous data file in EDIT you will see the comment at the time point at which you initially clicked the annotation button This option will become active during data storage only Clear Average The Clear Average option will clear the accumulated sweeps in an online average file It may also be accessed by clicking the right mouse button between electrode displays in a Multiple Windows display for online averages Send Waveforms to Waveboard This option will send waveforms to the Waveboard Itis also accessed by clicking the right mouse button between electrode displays in a Multiple Display Window for an online average It is not necessary to start the Waveboard first it will be opened automatically Please see the Waveboard Appendix at the end of the EDIT manual for a complete description of its operations Acquire 64 channels File Edit View Acquisition Options Window Help de Options The Options fields allow you to set a variety of personal preferences used in the display windows such as colors axis labels etc You may also save and later recall size and position information for the data win dows you open All of the settings are saved in Workspace files Options Window Help Multiple Window Settings Single Window Settings Load Workspace Save Workspace Video d Batch ACQUIRE4 2 165 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Multiple
178. ns without making any changes to existing settings Note You must click OK to save the position information before you click the Make Same Size button again Otherwise the new position information will be lost when you click Make Same Size and the electrode displays will return to their original positions Import and Export Positions Note that there are two buttons used for importing and exporting electrode position information This is the size and position informa tion of the Import Positions Export Positions electrode displays for the Multiple Windows Display You can export the informa tion from one data file and then import it with a different but matching in terms of number of channels and channel labels data file For example if you have one complate setup file with the sizes and positions they way you want them and second setup file with sizes and positions you don t like you can apply the information from the first one to the second one Retrieve the first setup file and click the Export Positions button Enter a file name and path the asc ACQUIRE4 2 139 Copyright 2001 Neurosoft Inc Document number 2202 Revision B extension will be added automatically Then exit the display retrieve the second setup file and return to the Channel Layout display Click the Import Positions button and retrieve the ASCH file that was created above This will apply the size and position information from the first
179. ns that must be made using the Ampinstall exe program in the Scan4 2 folder The configuration is the same regardless of your Operating System 98 ME NT or 2000 Run the ampinstall exe program and see the following display SCAN 4 2 Amplnst Ge Net Acquire Broadcaster Net Acquire Receiver Name is a network identifier You can place any name you want in the field such as Lab Remote etc You will need to run ampinstall exe on each computer that you will be using and to set each one as either f Net Acquire Broadcaster the computer connected to the amplifiers or ZS Net Acquire Receiver the one or more computers connected to the Broadcaster over your network Click Done when you have made the selection Once you have made the appropriate configuration modifications you are ready to start ACQUIRE on the PCs Make sure a hardware lock or dongle 1s connected to each PC You must start the Broadcaster PC first ACQUIRE4 2 180 Copyright 2001 Neurosoft Inc Document number 2202 Revision B The network version of ACQUIRE 1s started from the Program Launcher by clicking on the Scan 4 2 ACQUIRE Network icon 7 The Main Screen of ACQUIRE will appear Acquire 256 channels OR File Edit View Acquisition Options Window Help For Help press F1 The Broadcaster PC will function as if it were any computer running SCAN 4 2 with the amplifiers directly connected Retrieve a setup file and pre
180. nt gains such as an artifact channel with a lower gain than the EEG channels In this case it is recommended that you determine the overall gain by using the majority gain of the EEG channels The Calibration procedure see Calibration section below will adjust a local scaling factor for each individual channel so you need not be overly con cerned with the degree of precision of the overall scale factor Generally lower gain settings are used with particularly high amplitude channels such as EMG from active muscle groups higher gains may be selected when looking at very low amplitude signals such as ABRs Range and Accuracy information corresponding to the selected gain settings are displayed in the adjacent fields to the right The Range values indicate the upper and lower voltage limits for each channel if the incoming voltage exceeds these limits the channel will saturate Accuracy refers to the precision of voltage measurement along the y axis The value displayed indicates in microvolts the resolution ability Ifthe accuracy 1s for example 087 uV then the voltage resolution will be in 087 uV steps This is similar to the dwell time on the x axis or in other words the time difference between adjacent data points Accuracy is the least measureable voltage difference between points Sixteen bits are available to describe voltage that is 2 to the 16th power minus discrete voltage values are possible per data point The resolu
181. nter the A D rate number of channels Acquisition type AC DC mode and gain and filter settings The display 1s divided into 3 major regions Acquisition settings Amplifier Settings and the electrode montage display Acquistion Amplifier Settings A D Rate 100 Humber of Channels 32 Reset Positions Acquisition Type Selected Channel E Dam Dm d Range 55m AccuUnacy 0 084 pW LSB Low pass op d High pass 1 00H2 d Apply To All Selected Channels Continuous AC DC Ge AC C DC DC Correction M Enable E Level Motch Enable Frequency Acquisition settings These settings control the basic parameters for acquisition A D Rate The digitization rate determines how often data are sampled by the analog to digital converters For SynAmps systems users may select from preset options ranging from 100 to 100000 To access the options click on the pull down arrow then use the up and down arrows to see the range of options ACQUIRE4 2 96 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Click on the desired value and this number will appear in the A D field Non SynAmps users Metrabyte and Labmaster cards may input integers from 100 to approximately 5000 from the keyboard The maximum AD rate that is available with the Metrabyte and LabMaster cards is highly dependent on the capabilities of your computer It may be more or less than 5000Hz If you e
182. number of seconds will be displayed on the screen Pressing the appropriate button on the tool bar will have the same effect Screen acceleration will not affect how fast data are digitized or written to disk However we do not recommend running the display at high speeds during data recording If you are display ing both the single and the multiple window displays please note that the single window display must be selected for the accelerate and decelerate options to apply Selection of either window display is made by clicking the mouse on the gray horizontal bar at the top of the display It will change to blue or other user selected color when active A note about display speed Depending on the speed and type unaccelerated of your graphics display card more or less time may be required to display waveforms on the screen Too fast ofa display may place a burden on the computer to keep up with the specified digitization rate This will be reflected in the buffer overflow line in the Status Box Ifthe percent of Buffer space being used rises in a monotonic fashion this is an indication that you are exceeding the limitations of your computer You should either decrease the rate of acquisition decrease the number of online fea tures or obtain a faster graphics card or computer Decelerate Display single window display only The Decelerate Display button will slow down the screen increased number of seconds displayed on the screen This
183. o adjust the Gain setting in the AD card Setup screen as described above If one or several values are too high or too low this is an indication of amplifier or other hardware malfunction As a final test of the calibration procedure record a sine wave signal of known voltage Then go into Edit and measure the peak to peak amplitude to verify it is the same as the original signal Itis not necessary to do this after you make the initial verification that the calibration procedure is yielding accurate values Calibration NuAmps systems The NuAmps are factory calibrated and require no further calibration Additionally no filter circuitry is built in on the pure DC amplifiers and NuAmps use very high precision preamplifiers with moderate gain coefficient 19 Such design eliminates the potential possibility to have serious inter channel differences in gain Variation among amplifiers is typically well less than 1 Saving Viewing the Calibration Values As mentioned above the calibration values are saved with the data files You can also save them separately in a text file To view the cal values that are saved with the data file go to Edit Overall Parameters to the Channel Attributes tab in ACQUIRE Calibration or EDIT The cal value for each selected channel is shown in the Calibration field fj gs75 To save the cal values to a text file from ACQUIRE or EDIT go to Edit Overall Parameters and to S librati En the Misc tab
184. o resize and reposition the display as desired ACQUIRE4 2 45 Copyright 2001 Neurosoft Inc Document number 2202 Revision B File Edit View Ac Step 11 Save the Setup file by clicking Save As F Setup use ABRSET again to add the Save Setup changes to the Setup file Step 12 To begin recording click Acquisition on the Main menu bar followed by Start Acquisition or just click on the green triangle toward the left end of the Toolbar P You will see the Multiple Window Display with one channel in it When you are ready to begin recording click on the Record icon the red dot on the Toolbar fr 11 andentera filename The avg extension will be added automati cally Click on Save and you will be returned to the Multiple Window Displays and in addition you will see the Average window display as well as the F data display In these windows you will see the average waveform develop and the F ratio and noise lines as they are generated Refer to the description of the Options section below m the Operating ACQUIRE part of the manual to see how to change display colors and to select different aspects of the window display Below is the resulting average using the Fsp option A Status bar will show the percentage of buffer space that 1s being used the amount of free space on your hard drive the number of accepted sweeps the number of rejected sweeps the Fs Block and the trigger type code of the last
185. omputer will not work ABOUT THIS MANUAL This manual is divided into two sections tutorials designed to illustrate the use of the program and a detailed description of the principles of operation The rapid and accurate collection of data from areal subjectisa skill that requires both technique and practice Therefore we strongly recommend that you read and carefully follow the tutorials and operations before you begin to collect any important data Should you experience technical difficulties with the operation of the SCAN software assistance may be obtained by contacting our e mail address techsup neuro com see Page 2 above for additional routes STARTING ACQUIRE For SynAmps users always make sure the SynAmp s is turned ON and that SN1 and SN2 SN3 etc appears BEFORE you boot your PC If you have not already installed your amplifiers and A D card when you installed the SCAN 4 2 software you should do so at this point you only need to do it once Run f 4mpinst from the Windows Explorer program The SCAN 4 2 AmpInst display will appear ACQUIRE4 2 9 Copyright 2001 Neurosoft Inc Document number 2202 Revision B SCAN 4 2 Amplnst Uninstall Name f Net Acquire Broadcaster C Net Acquire Receiver Done Click Install and the Select Amplifier and A D card window will appear The Network options are used if you are using the network version of ACQUIRE please see tha appendix at the end o
186. on the green Start Acquisition arrow J ongoing activity from up to 32 channels is displayed in individual windows The windows are labeled according to the information entered in the Channel Assignment Table ACQUIRE4 2 89 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Se TCE HI POW 1 reer male By clicking on any channel you may zoom in and monitor a channel with a larger sized window Note that when you position the mouse in one of the electrode fields you will see the corresponding electrode label ms and V values on the Status Bar at the bottom of the screen Note You may rearrange the positions and resize the boxes in the Individual Channel Settings section under Edit on the Main menu bar Changes saved in that section will be invoked during acquisition Right Mouse Button Options The right mouse button provides access to several additional options that are useful with the Multiple Window Display with online AVG files Position the mouse cursor between electrode display windows and click the right mouse button The following list of options will appear Clear Average Send waveforms to Waveboard Clear Average In any of the online average displays it is possible to clear the average by clicking the right mouse button inside the Average Window but outside the electrode channel displays Press the Clear Average option to clear the online average Send Waveforms to Waveboard When you sel
187. onnector On the STIM side use the same headphone jack on the front of the STIM audio system unit that you use for headphones In Sound create a click with 0 ms delay approximately 08ms duration with 100 of Maximum amplitude settings are available when you create the click Set the dB level to 130 dBs Check your stimulator manual for duration and polarity of the pulse Polarity is flipped by Scaling the channel using a 1 see under Transforms STIM will simultaneously send a TTL trigger to SCAN Use the Control box settings under Config to set these values H H Negative STIM will repeat the trigger 500 times with an ISI of 300ms A trigger type code of 10 will be sent to ACQUIRE Again these are suggested settings your actual values may vary depending on your recording paradigm TTL triggers Youmay also use STIM to send a TTL trigger to the SEP stimulator This is a little more involved because it requires splitting the STIM to SCAN cable The idea is to send low number bits to the stimulator and higher number bits to the SCAN system or vice versa depending on what the stimulator needs If you are looking at the back of the STIM connector of the STIM to SCAN cable pins 2 through 9 carry bits 0 7 Pin 2 is bit 1 pin 3 is bit 2 pin 4 is bit 4 pin 5 is bit 8 pin 6 is bit 16 and so forth By splitting the cable you can send low numbers such as 1 pin 2 on the back of the STIM connector to one system and higher numb
188. onse ABR it may be extend able to other types signals that are stationary in nature The F values are computed for a series of block averages These block averages are summed together to form an overall grand average In addition to computing the F statistic the contribution of an individual block average to the grand average is weighted by the background noise Bayesian weighting This has the effect of minimizing noisy blocks in the grand average The F averaging mode is particularly useful in recording ABR waveforms from noisy or uncoop erative subjects It provides feedback on the quality of the signal and minimizes the need to replicate waveforms Follow these steps to configure the system to record an ABR using the F averaging mode Step I Start ACQUIRE as describe above and you will see the ACQUIRE main screen renge Zb channel DL ts tjon emaz CIA eebe bes Step 2 Retrieve the SEPSET ast file from the prior tutorial or use the Make Default Setup option as described in the preceding tutorial We will modify the ast file for this demonstration Step 3 Click Edit from the Main Menu bar then click Overall Parameters A multi card window Edit View Acquisition Options Overall Parameters Individual Channel Settings Channel Assignment Table Make default setup Subject Synamps Hardwa
189. onventions The result might appear similar to the figure below Small Small You can also use the Adjust Positions buttons to reposition the electrode windows automatically The Small and Large designations allow you to set the displays to two sizes The Small size is the one that appears as the default size If you click inside the window to enlarge it to the mid size as opposed to double clicking to get the full screen display that intermediate size is the Large size which you may also set as ACQUIRE4 2 36 Copyright 2001 Neurosoft Inc Document number 2202 Revision B desired Click OK to continue Step 11 Now go back to the Edit option on the Main Menu bar and select Channel Assignment Table Phys Chn Label EE SE E st E if reem You will see the 4 channels as previously entered The order of the channels is contingent upon your actual cable connections and amplifiers refer to your physical installation manual for more details as well as the Channel Assignment table section in the Operating ACQUIRE section below If you have not already relabeled the electrodes you may relabel them here When you are finished click OK Step 12 Ifyou have made any changes since the prior Save step above be sure to Save the setup file File Edit View Ac again by clicking Save Setup under the File option __ 984 Setup from the Main Menu bar use Save Setup SEPSET again to add the changes to the
190. or combinations of channels you create with linear derivation files On the Startup page you may designate up to three dr files one for each Single Window Display and one for the Multiple Window Display Click on the Deriv button and Windows Open File utility will appear allowing you to select an LDR file that you have created The selected file will appear in the adjacent field Map File Name You can display the maps using any of the orientations you have created with 3DSpace or Mapgen Click on the Map button and an Open File utility will appear allowing you to select a map file map extension Click Open and the map file name will appear in the adjacent field If you are mapping dr data remember to create a map file that corresponds to the number of channels and new labels in the derived file Note as of the time of this writing it is essential that there be exact agreement between the labels in the map file and in the ACQUIRE setup file This means the case must agree Cz will not work with CZ and be sure there are no spurious spaces or other characters Data Source You may link the map to any of several files including the RAW data as well as the sorted averages for example Click on the down arrow to see what data files are available for mapping You need to be in Average acquisition mode or have sorted averages listed on the Sorting display prior to entering the settings on the Mapping page Video These buttons all
191. or the number of channels if needed derived from a modified 10 20 placement system In this example assume that the last two channels are the vertical and horizontal eye movement monitoring channels recorded from sites above and below one eye bipolar VEOG and to the left and right of each outer canthus bipolar HEOG Acquistion Amplifier Settings A D Rate 100 Humber of Channels 32 Reset Pasitions Acquisition Type Selected Channel 4 Gain Dm Range 55m Accuracy 0 084 pW LSB Low pass oun d High pass 1 00H2 d Apply Ta All Selected Channels For Acquisition type select Continuous This setting configures ACQUIRE to store data in a continuous format If you are using SynAmps under AC DC select AC mode If you are not using SynAmps this section will not be accessible Continuous kd Enable r Level T Enable Frequency Under Characteristics select Common Settings and enter a Gain of 1000 a Low Pass filter of 30Hz anda High Pass filter of 1Hz Leave the Notch filter OFF no check mark in the field Step 5 Now click the Channel Attributes tab Set the VEOG and HEOG channels as Artifact Rejection channels as 1n the previous tutorials ACQUIRE4 2 57 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Artifact Been Z S KS gelaut lilgeese Default Step 6 No triggering will occur in this recording The Hold value
192. ordings Set the Start time for 200 and the End time General Artifact Rejection Display Satins 20 Start rs 200 Start ms Lo Stop m Dom o IER End ms fi500 Y Min 100 Points SS Mia 50 Y Max 100 Avg Update H May uh 50 Te Autoseale Baseline Correction Enable Start ms fo End m P for 1500ms The sample interval has been set with a pre stimulus interval of 200ms and a post stimulus interval of 1500ms Since this is a continuous recording setting the sample interval will have no effect on data acquisition However itis convenient to have these parameters ready for offline epoching performed with the EDIT module Click on the Points field and the field should change automatically to 851 The Avg Update field is not used with continuous recordings The program is designed not to allow invalid settings for any of the fields Ifyou click on a field and get an error message saying to input a number within a certain range click OK and see what field has been high lighted It may not be the one you had clicked Enter the appropriate number in the field that was high lighted then return to the original field you had selected and continue Note the interaction between the A D rate and the number of points selecting the A D rate in the Amplifier section will affect the sample duration and the number of points in the Epochs section ACQUIRE4 2 51 Copyright 2001 Neurosoft Inc Document number
193. ormation and are stored as separate files with an AST extension Several setup files for NuAmps are supplied and these are found in the Scan4 2 Setup Files Nuamps folder It will generally work best if you select one of them and then modify it for your own needs The complete description for the use of NuAmps is found inthe NuAmps manual The primary differences with NuAmps are found onthe Edit Overall Parameters Amplifiers tab andthe NuAmps Setup option under Edit Amplifiers settings The differences here are that there is no AC option and the Gain and Range and Accuracy for NuAmps is always 19 see the NuAmps manual for more details The maximum AD rate is 1000 and consequently there are fewer options for the Low Pass filter Dam 13 bd Range 263 mi Accuracy 0 063 UV db NuAmps setup screen The main differences are seen on the NuAmps Setup display Click Edit on the Main Menu bar and select the NuAmps Setup option You will see the following display with two tabs These are unique to NuAmps Reference Trigger Test Set reference Current selection At x1 x2 Fpl Fp2 X3 X4 ew e e M e e C AT A2 2 Lett 2A2 Right 41 E Fight gt 42 Lett 2 gt 41 Av Virtual Ground W Show channel numbers h Show head contour ACQUIRE4 2 70 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Reference The Reference display lets you select what site s to use for the reference electro
194. ory brainstem response ABR by means of Bayesian inference Scand Audiol 14 89 Elberling C and Don M 1987 Detection functions for the human auditory brainstem response Scand Audiol 16 89 92 ACQUIRE4 2 178 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Appendix A ACQUIRE Network Version ACQUIRE4 2 179 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE Network version The network version of ACQUIRE allows you to display and process data on remote computers For example one version of acquire can be configured as the Broadcaster to send data over a network to anumber of remote Receiver computers These computers can all simultaneously view the incoming data just as though they were attached to an amplifier These remote computers might be configured to process the data in other ways One computer could perform an online spectral analysis while another could be used to perform online averaging and so forth The only limitations are the speed of both your host computer and the attached network Each connected PC MUST also have its own SCAN 4 2 license Full Acquisition only or Reader only with the software installed We will assume that you already have the PCs linked across the network with the appropriate permissions Setting up Network ACQUIRE is much simpler in SCAN 4 2 than in earlier versions To configure the Network ACQUIRE software there are minor modificatio
195. osoft Inc Document number 2202 Revision B Click OK if you wish to proceed and Cancel to exit without making any changes The option is useful if for example you have several errors in a setup file you are creating and wish to recreate it from scratch If you are trying to modify an existing setup file and encounter difficulties making it function properly try recreating the new setup file after selecting Make default setup Subject Clicking on Subject displays the Subject Information screen The top part of the screen displays Subject related fields in which you may enter the Name ID number and so forth The text fields at the right allow you to enter free flowing text to describe Medications levels of Wakefulness and other Comments Sex and Handedness buttons are in the middle of the screen At the bottom there are fields for Institution information and Session information The Date and time fields are added automatically Subject Information Subject Name pecie D Unspecified Medications Age O00 ge Po Wak efulness Height fo Weight fo Heen a Unspecified Language po Ethnicity Ir De Hand E Male Female Unspecified E RCL C Mixed te Unepeciied Session Date Unspecified Time Unspecified Temperature fo Comments Dave Load Clear Form PEE Institution Institute Unspecified Department E Researcher Unspecified Operator Unspecified Um Ir Store as Default Session Notes
196. ou to export your AST setup file as an SET file You may also create SET files from data files in the EDIT program see the EDIT manual Load Bitmap Click this option to retrieve one of the bitmap bmp snapshot files that you have saved this requires the use of the video camera Exit exits ACQUIRE Acquire 64 channels File Edt View Acquisition Options Window Help 1 Edit The Edit menu option is used to create or modify files that will configure the ACQUIRE program with preset parameters The Setup file is typically the first operation performed in ACQUIRE and will deter mine such variables as the number of channels digitization rate pre and post trigger intervals online ACQUIRE4 2 78 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Edit View Acquisition Options Overall Parameters Channel Layout Channel Assignment Table Make default setup Subject Sunamps Hardware features etc One or more Setup files that you create will ordinarily exist on your disk and selecting the desired one will greatly facilitate the routine acquisition of data Overall Parameters The Overall Parameters section allows access to the configuration options in ACQUIRE When this option is selected the Overall Parameters window will appear with several options labeled Startup Amplifiers Channel Attributes Triggers Epochs Fsp Average Frequency Sorting Audio Mapping and Events Each of these w
197. ou would like the display to be in a bar form histogram Histogram or in linear form Line Then if you are using Individual Sweeps or Running Average mode enter the number of sweeps to be included in the Update field During acquisition the FFT display will be updated after the number of sweeps you enter Next specify the frequency range that you would like displayed from the minimum X Start to the maximun X Stop This value cannot be greater than the Range value in the Window field above Select the y axis range of values using the Y Min and Y Max fields or the program can perform automatic scaling along the y axis in which the Y Max value will be adjusted according to the maximum value present in each sweep Copyright 2001 Neurosoft Inc Document number 2202 Revision B When you are satisfied with the settings you have made click Save as and the Windows Save File utility will appear These changes will be saved to the Setup file you designate ast extension will be added automatically After saving the settings click on OK to continue Click on Cancel prior to saving the settings and you will exit the window without making any changes to your setup file Note the online frequency analyses will always be linked to the raw incoming data You cannot for example do an online FFT of data undergoing online filtering Sorting The sorting option allows you to perform Online Averaging click Enable box to activate the page
198. ovide enough resolution to determine peak latency and amplitude of the major ABR components For this ABR example only one channel will be needed One electrode will be placed on the right ear lobe A2 and the other at CZ Enter 1 for the number of channels we will relabel it and reposition the display below Click the Reset Positions button to see the single channel the label has been renamed already in the figure below For Acquisition type select Fp Average This setting configures ACQUIRE to record with the F mode If you are using SynAmps under AC DC select AC mode Set the Gain to 2500 the Low Pass filter to 3000Hz and the High Pass filter to 100Hz Leave the Notch Filter OFF no check mark in the box Click the Select All button to select the channel or just double click the label and then click the Apply To All Selected Channels Y button to apply the settings ACQUIRE4 2 40 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Acquistion Amplifier Settings A D Rate 20000 Humber of Channels li Reset Positions Acquisition Type Selected Channel Ie m Gain Gem d Range Daw CCUACY 0 007 pW LSB Low pass 2000 Hz gt High pass DH d Apply To All Selected Channels gt i Fep Average Enable T Enable Frequency Step 5 Now click the Channel Attributes tab Relabel the single channel to CZ already relabeled in the figure above and
199. ow you to select which map you want to you on the overlay in the video display To use this option you must have installed a video camera on your PC Select a single map that you want to use or select None if you are not using a camera The complete video functioning is described above in the Toolbar icon section under the video related icons io ee p ip When you are using the online mapping feature you will see that as soon as you click a scaling arrow highlight a display etc the Active Map display will appear This will have buttons available for each map that you have selected for display Delta Theta etc You must have at least two online maps for the Active Map display to appear Active Map s K a D Weta Alpha Bete Beta To understand the function of the Active Map buttons you should first enlarge one ofthe channels in the Spectral Data display to full size You will see in addition to the power spectrum display two vertical dashed lines and two horizontal dashed lines ACQUIRE4 2 125 Copyright 2001 Neurosoft Inc Document number 2202 Revision B You can move the lines by grabbing them with the left mouse button and dragging them to a new position If you position the mouse between the two vertical lines and above the upper horizontal line you may drag both vertical lines at the same time thereby preserving the frequency interval between them With a power spectrum display the lower hor
200. pare for acquisition You should use the same setup file on the Receiver PC or one that has the same number of channels and same AD rate Start ACQUIRE on the Receiver PC and you will see the following display Select the Internet TCP IP Connection For DirectPlay and click OK Choose Connection Method sernal Connection For DirectPlay Modem Connection For DirectPlay IF Connection For Decke Internet TCP iF Connection For OirectPlay You will then see the Locate Session display Alternatively you may select the IPX Connection For DirectPlay to see a list of the networked PCs ACQUIRE4 2 181 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Locate Session Enter the computer name or IP address of the session host or leave blank to h Seed Cancel Enter the Source computer name or IP address or leave it blank as directed and then click OK It may take a few moments for the Receiver PC link up with the Broadcaster PC When they are linked select the setup file and start acquisition You will see the signals from the Broadcaster PC ACQUIRE4 2 182 Copyright 2001 Neurosoft Inc Document number 2202 Revision B
201. plays in Windows On the scrolling display you will see a Scale Tool ATE will rols as A a ik GC aaa aha iaaa You can change the color ofit and make it opaque if desired Close the Single Window display and go to Options then the Single Windows Settings option The Scale Tools options are at the lower part of thedisplay ACQUIRE4 2 81 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Scale Bow fe Opaque Transparent When you use the up and down arrows from the Toolbar to scale the waveforms the scale tool will be adjusted accordingly Also appearing 1s a Status box that provides important information during acquisition Status Fei Butter LU Wort DC Value 1 Free Space MiB 217 Recording Time 00 00 06 Event Count T Buffer This value shows what percentage of the buffer space is being filled during acquisition If this number remains consistently high it may be an indication that you are close to or exceeding the limits of your machine see 1 under Special configuration concerns for continuous acquisition below Worst DC Value This shows the greatest percentage of amplifier saturation Free space MB This value provides an ongoing indication of how much free space remains on your hard disk drive or other drive designated for storage Recording Time While saving the data the Recording Time indicates how long the saving process has been in progress Th
202. ps under Test Trigger Port above NuAmps to STIM cable testing Decimal Binary stimulus D Jecoonoo T Last Stim ja Jecoonoo Response ja 00000000 Counter ja Hold Value jo When you have entered the desired selections click the OK button Click the Cancel button 1f you want to leave the Setup section without applying any of the changes you made To save the setup file you just created or modified click File then Save Setup The standard Save As utility will appear in which you can specify a path and file name the ast extension will be added automatically Metrabyte Card If you have amplifiers other than SynAmps NuAmps or QuickTrace you will have received a Metrabyte AD board and an I O board described below DAS 1802 board Ifyou have the Keithley Metrabyte DAS 1800 A D card in the SCAN PC select that option and a Metrabyte DAS 1800 Setup dialog box will appear ACQUIRE4 2 72 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Metrabyte DAS 1800 Setup x Amplifier Gain 20000 Low pass Hz E High pass Hz 0 3 Trigger Qut Voltage Default v E 4 D Converter Range AYVto ay LO Base address hes 340 DMA channel Active W 5 e IAG number Amplifier Gain Enter the gain or attenuation factor for your amplifiers for example 20000 This may vary depending on your amplifier settings Low pass Hz Enter the low pass filter setting that
203. r 2202 Revision B Add Derivation Remove Wenvator Add Display Filter Remove Mepa Filter Channel Order Add Derivation Add Derivation allows you to apply an LDR transform to the CNT file during acquisition This is the same option that may be set up in advance on the Startup screen under Overall Parameters see the Derivation section above The applied LDR is for display purposes only Note if you apply an LDR file the additional display page function will be disabled Remove Derivation Click this option to remove the applied LDR transform Add Display Filter Clicking this option displays the Filter screen see the Filter section above and performs the same function as the Filter option on the Startup display under Overall Parameters except that it may be applied during acquisition Enter the settings as desired click the See ea eee 3 button and click OK to apply the filter to the dis played CNT file The filter will affect the display only Remove Display Filter Clicking this option removes the effects of the Add Display Filter option Channel Order This option allows you to reorder the sequence of channels on the CNT file display The same option may be selected from the Single Window Order button on the Channel Layout display Clicking it displays the following screen ACQUIRE4 2 87 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Single Window Display Order Ordered Displ
204. r example the 60Hz band is completely encompassed by the EMG band Lastly you may define the colors for each band by clicking on the corresponding button in the Color column The color palette will appear as described above Select the desired color and click OK to continue Misc In the miscellaneous field you may select additional display options Misc h Waveform Box T Overlay Waveforms M Display Electrode Labels Jh Show Standard Deviation M Show Head Overlay Copyright 2001 Neurosoft Inc Document number 2202 Revision B Waveform box This field toggles the Waveform Box on and off This is the line box that surrounds the electrode display The color 1s controlled by the Waveform Box color button Overlay Waveforms The Overlay Waveforms option applies to epoched files Enable it and as you step through the EEG file you will see successive sweeps overlain on the previous sweeps Display Electrode Labels This option will toggle the display of the electrode labels in the upper left hand corner of the displays Show Standard Deviation This field toggles the display of the Standard Deviation for averaged data files assuming the sweeps or files must have been averaged with the Compute Standard Deviation option enabled When averaging single sweeps that option is found on the Averaging transform display in the Options section When group averaging AVG files the Compute Standard Deviation option is found on
205. r space that is being used the amount of free space on your hard drive as well as additional information In general the buffer space should never climb beyong a few percent Ifthe buffer indicator does start to climb then this means that you are exceeding the data process ing capacity of your computer If the buffer continues to climb evenually you will receive a buffer overflow condition and data will be lost Should this event occur please contact Neuroscan Labs technical support It is also important to monitor amount of free disk space Itis not good practice to allow the system to fill up the available disk space Free space 1s particularily important for the system drive The free space in ACQUIRE4 2 38 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Windows is used to swap memory System performance can be degraded dramatically if this swap space is reduced Refer to the View section in the Operating ACQUIRE details below for description of the Toolbar options for controlling acquisition Termination will occur after the 500 sweeps are accepted or after user interrupt by clicking on the Stop icon Fsp averaging ABR SynAmps only The Fy averaging mode configures the system to compute an F statistic based on a single point estimate of background noise The F statistic can be used as a measure of signal reliability and waveform quality Although it was originally developed for use with the auditory brain stem resp
206. r the online display only Notice the relationship between the number of points and the frequency resolution the more points you allocate the finer the frequency resolution For Type of Windowing select Cosine and enter 10 for the percentage of the Duration of the epoch to be tapered Step 9 It is a good idea with EEG recordings to monitor the activity for indications of severe EOG artifact floating or otherwise bad electrodes or signs of increasing drowsiness This can be done by inspecting the waveforms alone but may be facilitated by watching online FFT results in mapping form To do this click on the Mapping tab and we ll create maps for monitoring delta and alpha Increased anterior delta will be an indication of increased EOG and other single electrode increases in delta are indications of possible bad ACQUIRE4 2 60 Copyright 2001 Neurosoft Inc Document number 2202 Revision B electrodes Decreasing posterior alpha in an eyes closed recording may be an early indication of drowsi ness Enable the Map 1 and Map 3 fields check marks will appear The first map is labeled Delta and the third map is labeled Alpha For the delta Min and Max enter 0 and 50 for alpha Min and Max Label Min Max Domain Start Stop LDA File Name Map File Name Data Source Video M Mei Deta o Jan M Freg 0 bar Deme Original Map kee Raw zl M Map2 Theta In 0 E Freq 3 508 Deriv Driginal fie Fav dl e M Mapa alba fo o
207. range that is calculated and available for display and is dependent on the number of points and digitization rate Duration 1s equal to the number of points divided by the digitization rate as described previously and is the duration of the sweep used for the online FFT ACQUIRE4 2 119 Copyright 2001 Neurosoft Inc Document number 2202 Revision B ACQUIRE4 2 120 Length refers to the extent of the windowing taper A typical value is 10 specifying that the extent of the windowing occurs at the upper and lower 10 of the sweep Duration Click on the pull down arrow at the Type field and select from the options the type of windowing you wish Current options are Blackman Cosine Hamming Hanning Parzen and Welch Acquisition Mode The acquisition mode lets you determine whether you want the FFT calculated for every sweep Individual Sweep option or whether it 1s to be calculated across a series of sweeps Ensemble Average An ensemble spectral average is created using a series of processing steps First time domain EEG epochs are windowed and converted into the frequency domain using an FFT algorithm Second power spectral estimates are computed from the real and imaginary results of the FFT Third the power spectral estimates are averaged together Ensemble averaging of spectra generally will improve the accuracy of the estimates of the EEG frequency assuming that the EEG spectrum 1s relatively stable and not in flux as
208. rd instead of down Ifyou have multiple display windows open only the one that is highlighted will be affected ACQUIRE4 2 147 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Restart acquisition Ei Clicking this button allows you to restart data acquisition For example if you have begun recording data and an interruption occurs you can restart acquisition with this option You will be prompted to re enter the file names of files to be saved Start Impedance Display 7 This is a Toolbar shortcut for going directly to the Imped ance testing routine described in more detail below Measure impedance of negative electrodes With bipolar recordings there are positive and negative electrodes depending on how they are inserted in the headbox The default measurement is for the positive electrode which 1s the active electrode for unipolar recordings Use this button to measure the impedance of the negative electrodes during Impedance testing Calibration al This icon accesses the Calibration function Please see the Calibration section below for more details Type annotation A This feature allows you to add text remarks in the continuous recording to designate any significant events Clicking on the button will display a window in which Enter Annotation EM ype a comment og you may insert comments The comments will be seen when you replay the data file in EDIT at the point at which you i
209. re will appear with the Startup card on top For this recording let s monitor the ongoing EEG channel in a ACQUIRE4 2 39 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Multiple Window Display and view the ABR average as it builds Enable the Multiple Window Display Single Window Display No 1 Single Window Display No 2 AA l Enable P ten wispa P liesen Multiple Window Display Ay Pye l ee Een Age tye If Enable 7 Filter Display Derivation Ueryaton File Sorted Average Window Display Spectral Display Au IT Enable d T Enable Negative Up Ge Down M Display this Menu on Startup D Auto Save check mark will appear and make sure no other displays are enabled Select the desired polarity Nega tive Up or Down For illustration s sake enable the Display this Menu on Startup field Now whenever you click on the green acquisition arrow or select Start Acquisition under Acquisition on the Main menu bar the Startup dialog window will appear The purpose of this option is to save you steps if you are acquiring data under conditions where you want to change the Displays between recordings you don t have to go back into the Overall Parameters option under Edit to make the changes Seb Weoley alter Step 4 Now click the Amplifiers tab Set the A D rate to 20 000 the maximum AD rate for LabMaster and Metrabyte cards will not be fast enough to do ABRs Digitization rates of 20000 Hz will pr
210. recognizes the labels and positions the electrodes accordingly This feature is particularly useful when you are creating setup files from scratch such as after clicking Make Default Setup or in any circumstance in which the electrode positions are not there such as when you import data files with no position informa tion The new positions will be transferred to the Channel Layout display when you click OK The Losd 3D pos button allows you to retrieve and apply position information obtained from a 3DD file created inn 3DSpaceDx This will allow you to reposition the electrodes in cases where the labels are not from or extend beyond as with 128 or 256 channel data files the 10 20 system labels The electrodes will be positioned according to their locations when they were digitized Retrieve the 3DD file and click OK to transfer the position information Lastly when you manually reposition an electrode you may see the Polar coordi nates change but not the Cartesian coordinates Click the Polar to Cartesian button to compute the Cartesian coordinates automatically The Cartesian to Polar button will do the converse Reset Positions Make Same Size When one of the electrode channel boxes is highlighted you may increase its size by clicking on the display area It will change from SMALL to LARGE or vice versa The Small size is the size that the display will have by default The Large size is the size it will be when you cl
211. requencm Ae 20 Cancel Enter the amplification value in the Gain field in the AD card Setup screen This value needs to be as close as possible to the actual gain of your amplifiers You should consult your amplifier manual or vendor to make sure of the settings or use an oscilloscope to verify that the output is in the 1V range by trying different amplifier gain settings Ifyou are still getting incorrect calibration Hetrabpte DAS 1800 Setup Amplifier ArD Gain 20000 ie Low pass Hz E or High pass Hz 0 3 1 T Top Out ic PS values in the ACQUIRE calibration routine 1 e something other than approximately 1 s as de scribed below then you may need to adjust the Gain setting entered in the ACQUIRE AD card settings Deviations can occur as a result of imprecise attenuation or amplification settings With some amplifiers it may not be possible to determine the exact output voltage of the amplifiers using the settings on the amplifiers front panels or even from the vendors If you are not sure of the actual amplification gain use a mid range setting on your amplifiers and adjust the Gain in AC QUIRE to get 1 s for Calibration values If the calibration values are incorrect and you are unsure what the Gain value should be try the following Calibrate using a Gain of 10000 Divide the Gain value you have entered by the calibration values you obtain For example if the Gain is 10000 and the calibration valu
212. rior tutorial set the VEOG and HEOG channels to be Artifact Rejection channels Double click the VEOG and HEOG labels so they turn green and make sure the Multi Channel Set field is displaying Artifact Rejection A check mark will appear in the Artifact Rejection field MR Artifact Rejection During acquisition and in the offline data files you will see an asterisk at the end of each electrode label on the artifact rejection channels To illustrate another feature let s change the color of the VEOG and HEOG channels Click once on the Selected Channel VEOG label or use the Frege 4 field to select the VEOG channel Then look at the Colors area tis divided into Single and Multi columns corresponding to the Single Window and Multiple Window Displays On the Startup page we selected a Multiple Window Display Therefore click the Custom button under Multi Click the button to access the standard Color selection display Select a color and click OK This will change the color for the VEOG channel waveform Repeat the steps to change the HEOG color ACQUIRE4 2 23 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Step 3 As in the prior tutorial triggering for the P300 will be controlled by an External device typically the STIM system across the parallel port Click on the Triggers label to access this option the only option with SynAmps and NuAmps E nten Dleiveldei The Hold value should be s
213. rough your ACQUIRE4 2 122 Copyright 2001 Neurosoft Inc Document number 2202 Revision B PC s audio system Hint to do this most effectively use the online filtering feature to reduce all activity other than the EMG potentials To use this feature you must have DirectX installed supplied with the SCAN 4 2 CD and a sound board in your PC Audio parameters are determined from the Audio page W Enable Audio Channel Status Sound channel Electrode Ge Left T3 C pr Frew Select whether the sounds are to be played on the left right or neither speaker for each EEG channel to be monitored You may select a single channel for audio presentation by clicking on Next or Prev until you see the desired channel in the Electrode window then select either the Left or Right speaker Alternatively you may select the channel or combination of channels from the display on the right Double click on the label to change the state of the desired channel s The default state is for all channels to be Off When you click a channel On double click on the channel the default speaker is the Right one As you step through the recording channels you can select which combinations you want for the Left Right or neither Off speakers Mapping The Mapping page sets the parameters for online mapping of EEG or EP data You may use map files that were created in 3DSpace or with MAPGEN Online mapping of EEG data is linked with th
214. rpose 2 Display fewer windows ACQUIRE gives you the potential to display the data in multiple windows and with multiple analysis features during acquisition however limitations imposed by your computer s abilities may not permit all of them at the same time 3 Obtain faster hardware Finally you can of course obtain faster hardware The 330kHz card uses a fast DMA channel and transfers data in 16 bit words instead of two 8 bit bytes easily doubling the throughput of the earlier 100kHz card however even higher rates can be achieved with the SynAmps system that has been optimized for continuous data acquisi tion You can also obtain a faster display card There are a number of inexpensive display cards that have been optimized for graphics interfaces that can significantly increase waveform display Or you can get a faster disk The disk drive is often the major bottleneck to continuous acquisition Creating EEG epochs from continuous data files Continuous data files created in ACQUIRE can be transformed into epoched data files offline with the EDIT program EDIT uses event markers placed in continuous data files to locate event locked epochs of data A continuous file can be trans formed into an epoched file by two different means You can create an epoched file directly from the continuous file or you can create an event file first and then create an epoched EEG file An event file is an ASCII file containing a byte offset from
215. s below this value will be attenuated This value should correspond to the actual setting on the amplifiers Note Users frequently acquire data with a very wide range in filter settings e g 0 1Hz high pass and 100Hz low pass Additional filtering and refiltering can be performed off line without affecting the original data Trigger Out Voltage Default resting V The Trigger Out is available for the control of external devices Typically the resting voltage is 0 0uV but you may set it to any value up to 5 0V Active V The active state of the Trigger Out may be set to any voltage up to 5 0V depending on the requirements of the external device ACQUIRE4 2 75 Copyright 2001 Neurosoft Inc Document number 2202 Revision B A D Converter Range The A D converter has a default setting of 10V but may be set to receive signals in the 5V range to provide better resolution of relatively low amplitude signals T O Input Output Base address hex Select from among the choices the correct base I O address hex value Typically this will be 2c0 unless there are conflicts with other base I O addresses Other settings will require corresponding changes on the LabMaster card refer to LabMaster documentation DMA channel The available options are 5 6 and 7 The DMA channel is typically 6 If there 1s a conflict with other hardware select a different DMA value no physical changes to the card required IRQ number S
216. s is the Pause button I If you click this during acquisition the display will be paused and data collection will continue The purpose is to allow you to examine a feature of interest without disrupting the data storage This is opposed to the Stop icon the black rectangle Mm which will close the current data file You will get a warning message during storage when you get to the last 10MB of space on the hard drive Storage will cease when you reached the last SMB of free space ACQUIRE4 2 146 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Use of Peripheral Devices for Data Storage We do recommend that you do NOT acquire data directly to a peripheral storage device Communication with these devices tends to be relatively slow and you will likely encounter data over flows or lost data Copy files to the peripheral storage devices offline With SCAN 4 2 the offline analysis software EDIT can read data files directly from write once read only CDs Increase scale factor tl Single Multiple and Frequency displays Increases amplitude of the signals in the display only Ifyou have multiple display windows open only the one that is highlighted will be affected Decrease scale factor Single Multiple and Frequency displays Decreases amplitude of the signals in the display only If you have multiple display windows open only the one that is highlighted will be affected Autoscale J Multiple Window D
217. s recom mended in this example If faster stimulation rates are desired you can decrease the sample interval as long as the post stimulus interval is of sufficient duration to view the major response components at least 10 ms The program is designed not to allow invalid settings for any of the fields If you click on a field and get an error message saying to input a number within a certain range click OK and see what field has been high lighted It may not be the one you had clicked Enter the appropriate number in the field that was high lighted then return to the original field you had selected and continue Note the interaction between the A D rate and the number of points selecting the A D rate in the Amplifier section will affect the sample duration and the number of points in the Epochs section In the Baseline Correction area click Enable check mark will appear and set the Start time to 5 and the End time to Oms Baseline correction will be performed online by computing the mean offset value from 5 to Oms and then by subtracting this value from the entire waveform Enable the Artifact Rejection feature by clicking on the field so that the check mark appears Enter the Start Sms and End 15ms and the Minimum 50uV and Maximum S0uV voltage settings Values that exceed 50uV will cause the sweep to be rejected In the Display area set the X Min to Sms the X Max to 15ms the Y Min to 20uV and the Y Max to 20uV You w
218. s recording 1s required for such tasks The disadvantage to continuous recording is that it can depending on the number of channels and digitization rate generate large data files However this limitation is becoming less of a problem with the rapid improvements in data storage devices optical disks and gigabyte hard drives To illustrate continuous recording we will configure the system for a visual N400 paradigm In this para digm a list of words in the form of a sentence is presented one word at a time on the screen Each word will send a sequence code via the parallel port to the ACQUIRE module These codes will precisely mark the onset of each word and will appear at the bottom of the continuously scrolling data screen Simultaneously the continuous data file will be written to disk For half of the sentences the terminal word will be a highly predictable semantically correct word congruent ending The other half of the sentences will end in an unpredictable semantically incorrect word incongruent ending These two types of words will be indicated by different stimulus codes so that they can be sorted from each other during the analyses In addition the words leading up to the congruent incongruent word sentence prime will be identified as to their serial position in the sentence by additional stimulus codes We will also take advantage of having 2 Single Window Displays to view the raw incoming EEG signals as well as filtered incomi
219. shortly We can also set all the channels as Artifact Rejection channels Make sure that Artifact Rejection is displayed in the Multi Channel Set field then click the SelectAll button the labels should turn green You should also see a check mark by the field ACQUIRE4 2 31 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Properties Multi Channel Set Artifact Rejection Colors Single Mut Ge Default G Default C 3 C A C Custom Custom IT Skip Operations Auto Add Last Bad T Auto Add Default r Units Select All Deselect All Selected Channel ES C3 E Physical Channel Calibration Impedance f fo E Artifact Rejection Oooo oo IT Fsp stop M Hide Waveboard Step 6 Triggering for the SEP 1s best controlled by the STIM SOUND system that simultaneously sends a TTL trigger to SCAN and the SEP somatosensory stimulator Use the External option the only option with SynAmps and NuAmps Startup Amplifiers Channel Attributes Triggers Ge External Hold value la IT Use inverted values nemal Intera lel The Hold value should be set to 0 see the triggering section in the Operating ACQUIRE section below for special circumstances With a SynAmps the Use Inverted Values field is typically disabled Interfacing with an external SEP stimulator There are 2 common ways to interface with an external SEP stimulator as distinguished from the S
220. should be 0 see the Operating EDIT section for special circumstances where this may vary and the Invert field should be checked or not depending on your particular system with SynAmps it is typically disabled klpm tere Step 7 Click on the Epochs tab to set the parameters of the recording epoch The number of Sweeps ACQUIRE4 2 58 Copyright 2001 Neurosoft Inc Document number 2202 Revision B General Sweeps i Start re jo End rm 2550 Points 256 Avg Update fi Display Start rms fo Stop ms 2550 Y Mm 100 Artifact Rejection Enable Start me E End ms P Mm pi Lon May Jul 50 Y Mag 100 Y Autoscale Baseline Correction IT Enable Start mz D End rm P in the General section is not relevant in continuous recordings Set the Start time for 0 and the end time for 2550ms The sample interval has been set so the number of points 256 will be equal to a power of 2 Click on the Points field and enter 256 if not already set automatically Again these settings have no relevance for continuous recordings but will be useful for offline epoching with the EDIT module The program is designed not to allow invalid settings for any of the fields Ifyou click on a field and get an error message saying to input a number within a certain range click OK and see what field has been high lighted It may not be the one you had clicked Enter the appropriate numb
221. st Sweep Type line in the Status Box Click on the Record icon the red dot on the Toolbar ll toenter any file names and path designations Clicking on Save in the utilities window will exit the window and data storage will begin You will see the averaged display window as with any Sorting files after returning from the Save data step To stop data acquisition click on the Stop button black triangle on the Toolbar or the Stop option under Acquisition on the Main menu bar During acquisition you may need to switch the focus from one window to another to change for example the scaling or speed You may do this by either clicking on the colored bar at the top of the desired window or by clicking on Window on the Main Menu bar In the latter instance you will see a list of labels corresponding to the screens being displayed ACQUIRE4 2 95 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Window Help Cascade Tile Horizontally Tile Vertically 1 Single Window Display oY Average CScand 2 Qemolsample avg The one with the checkmark indicates which window is currently in focus Click on one of the other displays to change the focus This option is helpful in instances where you have several windows on the screen at a time and one becomes completely hidden by another You can easily bring the hidden screen to the front by selecting it with this option Amplifiers The amplifiers section allows you to e
222. ted Average Window Display Enabling this option 1s required for online sorting refer to the online Sorting description below When enabled you will see the sorted average accumulating in Sorted Average Window Display AN W Enable its own labeled window Note waveforms in this window will not appear until the first trigger is received At that point you may zoom in and monitor a channel with a larger sized window Note that when you position the mouse in one of the electrode fields you will see the corresponding electrode label ms and V values on the Status Bar at the bottom of the screen Spectral Display The online Spectral Display option is used in tandem with either the Multiple or Spectral Display d If Enable Single Window Displays It displays the results of an online FFT using settings you entered on the Frequency page under Edit Overall Parameters please refer to that section for a complete description C3 CF TCF GO ACQUIRE4 2 94 Copyright 2001 Neurosoft Inc Document number 2202 Revision B As with the Multiple Windows Display you may zoom in and monitor a channel with a larger sized window Note that when you position the mouse in one of the electrode fields you will see the corresponding electrode label frequency Hz and uV values on the Status Bar at the bottom of the screen The display may be set for Histogram as shown above or with line drawings Negative The Nega
223. th data another buffer is being written to disk If you see that the Buffer value seen in the Status box during acquisition begins to climb this is an indication that you are running out of buffer memory and that the computer cannot store the data fast enough to disk It 1s normal for this value to increase temporarily when the disk is accessed However 1f this value starts to increment consistently it is likely that you have exceeded the speed of your computer In that case you should either slow down the acquisition rate and or decrease the number of online functions Copyright 2001 Neurosoft Inc Document number 2202 Revision B Increasing your throughput There are a number of ways to increase the speed of continuous acquisition Listed below are some potential solutions 1 Defragment your disk Windows 95 98 only not NT How fast the system can write data to disk 1s a primary limitation of continuous data acquisition One factor that influences disk speed 1s how often it has to move the disk head s to a different location on the disk The disk can write data much faster 1f there is a large block of contiguous space available With continuous usage of your disk the large unused blocks become frag mented into smaller and smaller portions scattered throughout the disk and the real speed of the drive is significantly slowed A simple solution to this problem is to defragment the disk regularly with a utility designed for this pu
224. the Waveform control The blue head shape is the Head Overlay Zooming in on a channel gives the following display ACQUIRE4 2 172 Copyright 2001 Neurosoft Inc Document number 2202 Revision B The positions of the x and y axes are controlled by the Single X Axis and Single Y axis buttons such as the Top Baseline and Bottom options for the X axis In the above example the X axis 1s set for Baseline and the Y axis is set for Adjust Left The Tick Marks controls toggle the tick marks on and off toggle the tick mark labels on and off and set the interval between the tick marks The vertical blue line on the x axis around 0 uVs is the Time Zero mark it may be toggled on or off and the color may be selected This is seen on all of the channels on the Multiple Window Display not just when you zoom into one of them Note For printing purposes you may want to have all white backgrounds When you are satisfied with the settings click on the Save As button to add the information to the workspace file or to create a new workspace file you may want tp have more than one workspace file depending on the operations you routinely perform the aws extension 1s added automatically Click on OK to continue or click on Cancel to leave the page without applying any of the selections Single Window Settings The Single Window Options dialog box allows you to set parameters for the Single Window Displays These include whether or not
225. the Group Averaging Properties display Show Head Overlay When enabled this option will display the head overlay Polarity These fields allow you to set the polarity when the file 1s retrieved You can set it for From File to use whatever polarity setting 1s saved with the data file Or you can select Negative Up or Negative Down to override the data file and always present the polarity the way you want Illustrations of Display Parameters The following figures are presented to simplify the parts of the display that may be controlled independently using the settings described above Below is a Multiple Window Display The gray area between the electrode displays is set ACQUIRE4 2 171 Copyright 2001 Neurosoft Inc Document number 2202 Revision B KM Ea Ea JP TROT em K Edi Edi Eii D Faia Hi Hr ts de by the Window Background color control The white area within the displays is controlled by the Waveform Background control The black electrode label color is set by the Text control The gray color for the VEOG and HEOG channels indicates that these are Skipped channels The red color for the FZ label indictes that tihs was set as a Bad channel Labels that have an asterisk have been designated as Artifact rejection channels The dark green box around each electrode display 1s the Waveform Box it is toggled on or off by the Waveform Box field The color of the waveforms themselves is set by
226. the beginning of the file for each event in the continuous file that can be edited manually with a text editor For more details on epoching data files see the EDIT manual AC DC SynAmps only Indicate whether you intend to record in AC mode only or in DC mode with DC correction Ifa DC correction is desired input the percentage of offset ACQUIRE4 2 101 Copyright 2001 Neurosoft Inc Document number 2202 Revision B AC DC CAC Ge Dc DC Correction W Enable en Level value beyond which an automatic correction will occur e g 1f you enter 60 automatic correction will occur when the D C offset exceeds this value Note When you record in AC mode the AC coupled filtered are added as a secondary step The DC filtering still occurs and the issues that affect DC recordings are still present when you record in AC mode For example you can Still saturate the channels in AC mode It will appear however as a flattening out of the signal rather than a drifting of the signal out of range Notch The Notch field field allows you to apply a 50Hz or 60Hz notch filter during acquisition In general it is better not to use the notch filter 1f you can avoid it since it will filter genuine EEG EMG data in the same frequency range Amplifier Settings SynAmps only These fields allow you to set the Gain and Filter settings for the individual amplifier channels assuming you have a SynAmps The basic operation
227. tion For an external calibration source with the Metrabyte or LabMaster A D cards or with SynAmps you will be using a calibration signal that is independent from the SynAmps or AD cards Typically this 1s a signal that 1s included with your amplifiers for calibration or it could be from a signal generator If you are using a signal generator 1t should be capable of producing a biologically appropriate voltage such as 50uc This can be accomplished with an attenuator that you supply as described above Click on the Calibration option under the Acquisition menu and then click on the External button in the Calibration Settings menu Enter the peak to peak voltage in vc of the sine wave signal you are using for example 50uc The Attenuation value should be set to 1 if you are not using an attenuator If you are enter that value in the Attenuator field and the unattenuated value for the Sinewave Voltage Note that you can enter the exact voltage going into the headbox with an Attenuator value of 1 and the result will be the same The frequency of the signal is determined by ACQUIRE4 2 161 Copyright 2001 Neurosoft Inc Document number 2202 Revision B the external generator and is not entered in ACQUIRE Again it is assumed that the amplifier output to SCAN is approximately 1V for each channel 8 to 1 2 Vs Calibration Settings Source C intemal Attenuation li Ge External SIME Wave Voltage pi ppl al R
228. tion in milliseconds of the response window For the ABR a value of 2ms is typical Window Stop ms The Window Stop point determines the ending location in milliseconds of the response window For the ABR a value of 10ms is typical Sweeps per Block The Sweeps per Block value determines the number of sweeps that is collected for the ongoing within block average For the ABR a value of 250 sweeps is typical F Value Input the desired F value that when reached will terminate acquisition A typical value for the ABR is 3 1 Noise Level uV Input the desired noise level that when reached will terminate acquisition A typical value for the ABR is 0 02uV Display These fields are used to set the scaling range in the KR data display This information is saved as part of the avg file Display F May E Notze Was pi o F Max The F Max sets the upper limit of the range on the F value display A typical value is 20 although you may go lower for a more sensitive display ACQUIRE4 2 118 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Noise Max The Noise Max sets the upper limit of the Noise Level display A typical value is 0 2uV Frequency Clicking this option accesses the settings page for Online Frequency Analysis Click the Enable box On check will appear for this feature You can use Online Frequency Analysis with any Acquisition Type When enabled the options will be highlighted and you
229. tion is thus interactive with the gain The greater the gain the smaller the voltage step per bit and the greater the resolution Low pass For SynAmps systems selections of low pass filter settings are presented in a pull down window Click on the desired setting for ex ample a setting of 100Hz attenuates frequencies faster than 100Hz and passes frequencies lower than 100Hz Non SynAmps systems enter this value from the keyboard in the Metrabyte or LabMaster options under Edit from the Main menu These should not be changed unless you physically change the settings on the amplifiers and recalibrate Additional offline filtering options are available in the EDIT module High Pass For SynAmps systems selections of high pass filter settings are presented in a pulldown window Click on the desired setting for example a setting of 1 Hz attenuates frequencies slower than 1 Hz and passes fre quencies faster than 1Hz Non SynAmps systems enter this value from the keyboard in the Metrabyte or LabMaster options under Edit from the main menu These should not be changed unless you physically change the settings on the amplifiers and recalibrate Additional offline filtering options are available in the EDIT module Channel Attributes The Channel Attributes window 1s used to set a variety of parameters for individual or all channels The montage display part of the screen 1s used to select or deselect the electrode channels that you want
230. tive fields allow you to reverse the polarity on the display and 1n the stored files If you want the negative side of the y axis to go up from zero select Up If you want it to go down Select Down Negative C Up fe Down Display this Menu on Startup By enabling this option Display this Menu on Startup check mark appears you can have the startup screen appear each time you click on the green Start Acquisition arrow or when you click Acquisition from the Main menu bar followed by Start Acquisition The purpose of this is to save steps if you are obtaining several recordings with varying acquisition options Enabling this option avoids having to go to the Edit menu each time you want to change acquisition options in a single recording session Auto Save The Auto Save field KL Auto Save Will automatically open a Save File Utility at the beginning of acquisition saving the step of clicking on the Save File button When you have finished entering the settings in the Startup page click on OK then the green Start Acquisition arrow or Acquisition on the Main menu bar to begin data acquisition The enabled display window s will appear and you will see the incoming signals When you start the STIM system or other stimulus presentation system with acceptable trigger codes you will see the stimulus and response type codes appear on the Single Window Display s In the Multiple Win dow Display the type codes will appear in the La
231. to the duration of a recording the number of minutes of EEG for which there is hard disk space and stimulus and response event mark ers are all displayed on the continuous screen Data can be replayed offline with the EDIT module Additonal information for controlling the continuous display and further considerations when recording in continuous mode are detailed in the View and Special configuration concerns for continuous acquisition sections below Epoched Inthe Epoched mode single sweep epochs of sample waveforms are stored to disk see Multiple Window Display above with an eeg extension The system checks to make sure there is enough space on the disk for the requested number of sweeps before acquisition begins Sweeps are initiated according to the trigger mode selected in the Trigger section described below Individual sweeps can be initiated internally INT or externally EXT Internally generated sweeps are paced according to the value in the interval field A synchronization pulse 1s sent out at time zero during the acquisition of the sweep Externally generated sweeps are initiated whenever a voltage value exceeds the predetermined values on a selected channel During acquisition the screen will display the current total number of sweeps This number will increment as sweeps are received The Status box will display the trigger type value of the last sweep The type value is the number read from the trigger port For example
232. uired to make this quality decision have been developed so far for the auditory brainstem response ABR only Elberling and Don 1987 However this method can be applied to other types of signals as long as the appropriate degrees of freedom have been determined and validation of the statistical independence of the single point have been met When this mode is selected a block by block estimate of the F is displayed in a window adjacent to each averaged waveform Averaging in this mode is performed in a blocked format In the blocked format two ongoing averages are maintained by the system the within block average and the between block average The within block average reflects the current ongoing activity as it is accu mulating The between block average 1s the grand average of all the Bayesian weighted within block averages For the ABR waveform 200 or more sweeps are typically aver aged together to construct a within block average The F statistic and background noise are estimated for each block and displayed at the completion of a within block average The current within block average is then weighted by a Bayesian function of the background noise and added to the between block grand average The Bayesian function is the recipro cal of the background noise for the current block divided by the sum of the reciprocal noise for all blocks This has the effect of minimizing the contribution of noisy blocks to the grand average Elberling a
233. umber of Channels Enter the number of 32 SEET Pasitions EEG channels that you will be recording Reset Positions This button is used to restore the electrode display positions to their default settings The default positions are a matrix of the display windows where the electrode channels are place in numerical order on the basis of how they appear in the Channel Assignment table The Reset Position button is also used when necessary where you increase the number of channels beyond banks of 32 For example if you begin witha 32 channel setup file then add another SynAmps to get 64 channels and make the change ACQUIRE4 2 97 Copyright 2001 Neurosoft Inc Document number 2202 Revision B in the Number of Channels field you may need to click the Reset button 1n order to see the additional 32 channels Acquisition Type Select the type of file that you wish to record Continuous Continuous Fap Average Continuous Epoched Average or Fsp Average described below ACQUIRE4 2 98 Continuous In continuous mode the data are displayed in a scrolling EEG paper like format in the Single Window Displays described above In this display relative amplitude is on the vertical axis and time 1s on the horizontal axis Data already on the screen are removed from the display as new data are recorded and displayed in their place Files created with the continuous mode will have a cnt extension Information related
234. understood more easily with a couple of examples ACQUIRE4 2 105 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Selecting a single channel for a particular attribute Click the pull down arrow at the end of the Multi Channel Set field to see the list of attributes that may be assigned Select the desired attribute e g Bad Unless previously modified all channels will be deselected red Double click a channel to select it and assign the attribute the corresponding field will show a check mark such as kg Bad L Selecting multiple channels for a particular attribute Select the Multi Channel Set attribute to be applied such as Auto Add to Waveboard Thenclick the SelectAll button A check mark will appear beside the attribute M Auto Add Double click any electrode labels to deselect them if you do not want the attribute applied to those channels Additional Features Now let s look at the additional modifications that can be made from this window Properties The Properties area displays many of the attributes that may be modified for each channel Selected Channel As demonstrated above the Selected Channel field will reflect the channel that has been selected when a chanel label has been clicked The upa nd down arrow buttons can also be used to select a channel An individual channel is selected when it appears in the Selected Channel window You can also Rename the electrode label by overtyping the e
235. using the F option you can simultaneously acquire a simple online average acquired outside of the Fp program To do this assume that the trigger type code coming from the STIM or other system is a 1 In the Sorting screen click the lt lt Add Average button and enter a file name for the online average file the avg exten sion is added automatically Under Criteria set the Stimulus field to 1 and make any changes in the Parameters Artifact Rejection Display and Baseline Correction fields Then in the Sort file area click the Export button enter a file name and click the Save button Notice also that if you wish you could have two identical averages that are accumulating differing only in that one has for example Artifact Rejection enabled while the other has the feature disabled not shown in the Figure Do this by creating a second average file using the lt lt Add Average button and changing only the Artifact Rejection Enable field Then Export the Sort file again Step 9 It would be a good idea at this point to save the settings that you have made thus far Click on File Edit View Ac Save Setup under File __ Load zehun and label the Setup file ABRSET the ast extension will automatically be added Then click on OK Step 10 Click on Edit again at the Main Menu bar and click Channel Layout You should have one EEG channel labeled CZ For the one channel ABR recording the only input at this screen is t
236. w to change display colors and to select different aspects of the window display Hint use the Tile Horizontally option under Win dow on the Main menu bar to arrange the multiple windows automatically Refer to the View section in the Operating ACQUIRE details below for description of the Toolbar options for controlling acquisition Continuous recording and online frequency analysis and mapping spontaneous EEG The spontaneous or ongoing EEG can be recorded in a continuous format and then played back and processed with the EDIT module This mode could be used for example to average around visually recognized events such as motor responses back averaging spikes or alpha onset Other uses might be spectral event related desynchronization or coherence analyses The setup for a spontaneous EEG recording 1s similar to the N400 example given above except that the epoch size and number of points will ACQUIRE4 2 54 Copyright 2001 Neurosoft Inc Document number 2202 Revision B be preconfigured for frequency analyses no sorting 1s involved and we will also configure acquisition for online frequency analyses and online mapping Follow these steps to configure for spontaneous EEG recording Step I Start ACQUIRE as described above and you will see the ACQUIRE main screen Aelia Zb channel A ISelpeiumgngtlitzzlftpr Jmipmaolsgzrl eisskeltiesr Fox Heip zen F1 ini D ss every BEERS Sa Step 2
237. ynAmps systems are limited to 65k points per bank of 32 channels Thus a 128 channel system 4 SynAmps can still allocate up to 1024 points for each channel Note the number of points restrictions apply only to averaged and epoched modes during acquisition Continuous recordings are not limited to a specific number of points only by the amount of available disk space Likewise epochs created offline by the EDIT module from continuous recordings are not constrained by the 65k point limitation Epoch size is limited here only by the amount of extended RAM Avg Update The number entered in this field specifies how often the online average file 1s updated If example you wish to have the accumulat ing average waveform recomputed after every 5 sweeps enter 5 in this field This field is only accessible when you have selected Average for the Acquisition Type under the Amplifiers section described above under Edit Overall Parameters Baseline Correction Baseline correction 1s performed by estimating an averaged value D C offset for part or all of the sample epoch The average value Baseline Correction M Enable Start me E DN End me jo is subtracted from each point in the sampling epoch for each electrode The portion of the epoch to be used in estimating the D C offset is specified by the Start and Stop points see below Enable The Enable option is used to turn on off the baseline correction option while making an
238. zed by grabbing one of the small boxes at a corner and dragging the box to a new size The box can be rotated by grabbing the box coming from the line in the middle of the overlay and dragging it to a new orienta tion The entire box may be moved by clicking inside the box and dragging it to anow location Next we need to set the Mapping tab to map the desired activity using the appropriate map file Go to Edit Overall Parameters Mapping ACQUIRE4 2 149 Copyright 2001 Neurosoft Inc Document number 2202 Revision B Label Min Max Domain Start Stop LDA File Name Map File Name Data Source Video Hone In this example we are mapping alpha activity using the supplied Lhead map file The map file that you use must match the labels used in the setup file r LS 0 E Now start acquisition in this case we used Continuous mode and a Single Window display and you will see the map displayed in its usual window as well as the same map superimposed on the camera display You will likely need to position and size the map so it lies meaningfully on the camera display You may also need to scale the map display play gs Real Time Video As the EEG scrolls you will see the map change on the camera display With that as a basic demonstration of the capabilities we will now look at the remaining options Many of these are accessed using the Toolbar icons while others use ACQUIRE4 2 150 Copyright 200

ACQUIRE 4.2

Contents

Download Pdf Manuals

Related Search

Related Contents