SoftGrip Multi-Channel - English
Contents
1. A 5 place balance should be accurate to within 0 01 mg and a 6 place balance should be accurate to within a 40 001 mg All items used to perform the calibration must be allowed to equilibrate to room temperature 20 25 C constant to 0 5 C Each time you recalibrate the pipette initial and date a new calibration label and replace the current label Also in accordance with your organization s instrument operation practices and standards we recommend following 2063 01 Rev E www hamiltonpipettes com 5 of 12 good laboratory practices by calibrating your pipette on a regular basis i e three month intervals using the following procedure or a known standard method such as DIN 12650 Depending on the frequency of use and the liquids measured other time intervals may be more appropriate To Calibrate 1 Attach a disposable tip to a chosen channel a middle channel is recommend and pre rinse the tip 2 Set the desired or the lowest volume at which the pipette will be used 3 Make 10 measurements of distilled water per tip recording the weights Adjust for environmental factors e g temperature humidity and altitude 4 Calculate the mean and standard deviation 5 If necessary adjust the volume However adjust the volume only after confirming the error is not due to other causes Refer to the sections on Optimizing Performance and Troubleshooting Adjusting the volume will affect all channels a Remove the calibra2. At 10 of At 100 of Pipette Volume Pipette Volume Accuracy Precision Accuracy Precision Volume Color within within within within Part No Range Coding 55019 50 5 50 uLx8 Sandstone 2 00 2 00 0 80 0 35 55019 52 30 300 uLx8 Brick Red 1 20 0 70 0 50 0 30 55019 54 5 50 12 Sandstone 5 00 2 00 1 00 0 50 55019 56 30 300 12 BrickRed 2 40 0 70 1 00 0 50 Optimizing Performance Topic Action Consistent Technique Pipette with a consistent rhythm pressure and speed Tip Selection Use Hamilton pipette tips for guaranteed accuracy For information visit www hamiltonpipettes com Tip Size and Fit Use proper size tip firmly placed on pipette shaft Pre Rinse Tips Pre rinse pipette tips for improved precision Sample Aspiration Keep the disposable tips immersed in fluid during aspira ion Do not let the plunger snap back to starting position mmersion Depth aintain an immersion depth of 2 to 4 mm mmersion Angle aintain an angle of 20 from vertical Viscous Samples Aspirate slowly If bubbles are observed resample Volume errors may still occur Refer to the Calibration section Acid Samples Pipetting strong acids and corrosive solutions is not recommended These liquids may damage the pistons and seals To protect the pipette use filter barrier pipette tips and inspect piston frequently For more information visit www hamiltonpip
3. by U S Patent Number 5 983 733 with other patents pending 20f 12 888 525 2123 2063 01 Rev E Hamilton SoftGrip Pipettes Hamilton Company is the leader in precision fluid measurement products We extended our world renowned syringe expertise to the SoftGrip Manual Pipettes specifically designed to deliver maximum accuracy and minimize hand injuries The results set the standard for precision comfort and value SoftGrip multi channel pipettes are continuously adjustable volume pipettes and available as 8 or 12 channel models for pipetting into 96 well plates The pipettes feature a soft nonslip handle for a relaxed grip lighter plunger action for more comfort and a separate tip ejector to reduce hand motion The curved hilt supports the pipette for finer control while pipetting The easily activated tip ejector lever releases all tips with a single stroke The ejector lever rotates to a comfortable setting for left or right handed use with a simple turn of the lower body adjustment ring and tip ejector Each channel has a separate piston and seal The hardened stainless steel pistons are precision ground and polished to ensure accurate performance and provide exceptional piston and seal life SoftGrip pipettes operate on the air displacement principle and use disposable tips Every pipette is calibrated at the factory with standards traceable to N I S T and shipped with a Certificate of Calibration documenting the pipet
4. by recalibrating your pipette after replacing any parts Pipette Recertification and Repairs Recertification Hamilton Company offers a program to recertify the accuracy and precision of your Hamilton pipette Testing is performed at a Hamilton facility under stringent conditions on state of the art equipment The calibration process is performed with an unbroken chain of calibrations traceable to N I S T Your pipette will be returned to you with a new Certificate of Performance If a pipette must be repaired to bring it into conformance you will be contacted with a cost estimate Repairs Hamilton Company offers a pipette repair program in addition to its general 12 month warranty You may return your Hamilton pipette for evaluation A series of basic tests will be performed to evaluate the pipette s performance If repairs are required to assure and confirm that your pipette is functioning to original Hamilton specifications you will be contacted with a cost estimate and the additional repairs and recalibration will be performed upon your approval Your pipette will be returned to you with a new Certificate of Performance For pipette recertification and repairs in the U S A call 1 888 525 2123 For authorized service outside of the U S A contact your local Hamilton Company office or representative using the contact information on the back cover 2063 01 Rev E www hamiltonpipettes com 9 of 12 Return of Goods Hamilton ret
5. SoftGrip Multi Channel Pipettes User Manual JL HAMILTON THE MEASURE OF EXCELLENCE 1 of 12 2063 01 Rev E Welcome Thank you for choosing a SoftGrip pipette from the Hamilton Company Before using your new pipette please review the important information in this manual on pipette features basic operation calibration optimizing performance maintenance and troubleshooting For French German and Spanish translations of this manual please visit www hamiltonpipettes com For sales and support in countries outside the U S please contact your local dealer For additional information in the U S call Hamilton Sales and Support at 1 888 525 2123 and visit our website at www hamiltonpipettes com www hamiltonpipettes com Sales Support USA 1 888 525 2123 Pipette Warranty Hamilton Company unconditionally guarantees its products to be free of defects in materials and workmanship for one year from date of delivery Any product that fails due to such defects will be repaired or replaced at our discretion without cost provided the device is returned with an explanation It is the responsibility of the purchaser to determine the suitability of application and material compatibility of the products based on the published specifications of the products The specified accuracy and precision performance of Hamilton pipettes is guaranteed only when used with Hamilton Precision Pipette Tips SoftGrip pipettes are protected
6. arts Table lists all pipette replacement parts identified by Hamilton part numbers To replace parts refer to the Pipette Parts Diagram 1 Snap the front and back covers off of the pipette If necessary insert a flathead screwdriver into slot on the side of the lower body and rotate gently 2 Unhook the two spring clips securing the ejector plate 3 Remove the ejector plate 4 Using a Phillips head screwdriver remove the screw that secures the piston chamber assembly and gently remove piston chamber assembly 5 Lightly lubricate entire circumference of pistons using a soft lint free cloth Lubricant Part No 5763 01 6 Insert and slide the pistons into the new piston chamber assembly Make sure that the seal flanges are oriented towards the plunger with the O rings below the flanges towards the tip 7 Adjust the position of the piston chamber assembly so that the screw on the back goes into the large end of the opening on the opposite side With the screw side facing you slide the piston chamber down so the screw goes to the very bottom of the opening and tighten the screw 8 Reinstall the ejector plate with the longer lip facing the front the side without the screws and secure the ejector plate with the spring clips 9 Snap the front and back covers back together To assure that your instrument meets the original Hamilton Company standards and specifications we strongly suggest that you follow Good Laboratory Practices
7. ettes com High Vapor Pressure Samples Pipetting solutions with high vapor pressure is not recom mended These liquids may damage the pistons and seals Sample Temperature Fluids at a temperature other than that for which the pipette and pipette tips have been calibrated may result in volume measurement errors Storage Store upright in stand Do not lay the pipette on its side with fluid in the tips Fluid reaching the pistons causes contamination and possible corrosion Cleaning Piston Wipe piston with alcohol and a soft lint free cloth Dry and lightly lubricate the piston Lubricant Part No 5763 01 Seal Replacement Replace the PTFE seals every 3 6 months depending on use and the samples aspirated Performance Checks Check the accuracy and precision of your pipette every 3 6 months depending on use and the samples aspirated 2063 01 Rev E www hamiltonpipettes com 70412 Troubleshooting Problem Possible Cause Recommended Remedy Inaccurate Results Pipette tip not attached Check for tip fit and check for correctly proper tip size replacing tip as needed Aspiration stoke too rapid Aspirate slowly by controlling viscous fluids rate of plunger release Calibration required Calibrate the pipette Seal leak dripping fluid Replace the seal Imprecise Results Inconsistent sampling See section on Optimizing method Performance Seal lea
8. into a safe receptacle depress the tip ejector to dispose of the tips Adjusting for Left Handed Operation The pipette is delivered ready for right handed operation Both the tip ejector and lower body can be easily rotated for left handed operation 1 The tip ejector lever can be rotated a full 360 relative to the handle by gently pushing the lever away from the handle While the handle assembly is lifted rotate the lever to the desired position and then release the lever 2 The entire lower body assembly can be rotated a full 360 relative to the handle by loosening the lower body adjustment ring Rotate the lower body to the desired position and retighten the lower body adjustment ring Pre Rinsing Tips Pre rinsing pipette tips is recommended for all pipetting operations To pre rinse aspirate and dispense the sample one to three cycles On the last rinse press the plunger past the blowout stop to clear the tips of any sample Make sure there are no micro droplets in the tips before aspirating the first sample If micro droplets remain in any tips replace the tips and pre rinse Pipette Calibration Hamilton SoftGrip pipettes can be gravimetrically calibrated by the user A universal calibration key is available so that the pipette can be adjusted to accommodate specific fluid viscosities and environmental factors such as temperature humidity altitude etc Use a 5 or 6 place balance depending on the volume being measured
9. k Replace the seal Cleaning Wipe the outer surface of the pipette with a mild soap and water daily In case of accidental contamination of the piston chamber shaft immediately disassemble the piston chamber Refer to the Replacement Parts section for instructions In addition rinse the ejector plate and piston assembly with mild soap and water followed by distilled water Dry all components completely Lightly lubricate the pistons Lubricant Part No 5763 0 I Autoclaving The entire pipette is autoclavable at 121 C for 15 minutes at 15 psi 103 5 kPa or 1 02 atm pressure without the need for any disassembly Although autoclaving should not affect pipette accuracy practice good laboratory procedures by checking your pipette and recalibrating as needed Pipette Parts Diagrams 5 Ejector Plate 2 Cover d B Header z 2 3 Spring Clip not replaceable Replacement Parts Table Part Description 8x50 pL 12x50 pL 8x300uL 12x300 pL 1 Plunger Cap 54190 06 54190 06 54190 10 54190 10 2 Cover 4917 01 4916 01 4917 01 4916 01 3 Spring Clip 34354 34354 34354 34354 4 Piston Chamber Assembly 6385 01 6385 01 6385 02 6385 02 5 Ejector Plate 4923 01 4922 01 4923 01 4922 01 8of 12 888 525 2123 2063 01 Rev E Replacement Parts Hamilton pipettes have been uniquely designed so simple repairs and part replacements can be performed by the user The Replacement P
10. t obtained from the graduated scale is to the right of the decimal point For the best accuracy and precision use your pipette only within the recommended volume range of 10 100 of the specific pipette s total volume range Recommended Volume Ranges Pipette Volume 50 uL 300 uL Pipette Color Sandstone Brick Red Recommended Volume Range 5 50 uL 30 300 uL 100 Volume Setting 50 00 uL 300 0 uL Reading at 100 Volume O 0 10 Volume Setting 5 00 uL 30 0 uL Reading at 10 Setting Smallest Increment of the Scale 0 02 uL 0 2 uL 4 of 12 888 525 2123 2063 01 Rev E Pipette Operation 1 Press disposable tips firmly onto the pipette s lower body assembly To assure an airtight seal gently rock the handle from side to side before lifting the tips from the rack 2 Depress the plunger button to the first stop Hold the pipette vertically and immerse the tips 2 to 4 mm into the sample below the meniscus 3 Slowly release the plunger button to aspirate sample 4 Withdraw the tips from the sample touching off on the side of the container 5 To dispense place the tips against the inside wall of the receiving vessel and depress the plunger button to the first stop Pause and then depress the plunger button to the second or blowout stop Hold the plunger button down sliding the tips out along the inside of the receiving vessel 6 Release the plunger button With the disposable tips directed
11. te s accuracy and precision Sloped Plunger Color Coded Soft Nonslip Handle Volume Display Color Coded Tip Ejector Lower Body Lever Adjustment Ring Diposable Pipette Tips 9 HD 2063 01 Rev E www hamiltonpipettes com 3 of 12 Volume Setting The isolated volume adjustment ring prevents accidental volume changes To set the volume hold the pipette vertical with the volume display in front of you and turn the volume adjustment ring to the desired volume Set the desired volume to within the pipette s volume range as given below For the greatest attainable accuracy always turn the adjustment ring down to the desired volume If you are starting at a setting lower than the one desired turn the adjustment ring past that volume and then turn back to the desired volume When setting volumes start your settings from the top digit on the volume setting display Full units are expressed in black digits Incremental units the values to right of the decimal point are expressed in red digits and appear below the full units The graduated scale identifies the least significant digit Notes For the 50 uL pipettes the red digits on the scale reading are to the right of the decimal point For the 300 pipettes only the last digi
12. tion label and locate the calibration reference mark b Remove the plunger cap by pulling the plunger button off the pipette c Inset the calibration key turning the calibration key clockwise to increase volume and counter clockwise to decrease volume Each vertical line on the calibration key is approximately 0 296 of the pipette volume d Remove the calibration key and replace the plunger cap Repeat the gravimetric procedure until results are satisfactory 6 Initial and date a new calibration label placing the label on the pipette hilt The pipette can be recalibrated for liquids with a density other than water Follow the calibration procedure above with the pipette set at the required volume Use the liquid to be sampled With this type of calibration adjustment the pipette is calibrated only for the liquid used and the volume tested The pipette should be labeled as such and should not be used for other sampling applications unless it is recalibrated Calibration Label and Key Calibration Calibration Plunger Calibration Key Label Reference Cap Showing Mark Vertical lines Test Conditions Liquid Deionized degassed distilled water Reference Temperature 20 25 constant to 0 5 C Number of Measurements 10 per channel per volume setting referencing ASTM 1154 Guidelines Pipette Tips Hamilton pipette tips 6 of 12 888 525 2123 2063 01 Rev E Performance Specifications
13. urn and repair policy is written to protect our employees from potentially hazardous materials e g infectious agents radioactive materials and carcinogenic chemicals or any substance that may cause them partial or permanent disability during the inspection or repair process When returning products please ensure the items are decontaminated and marked accordingly Furthermore the customer assumes responsibility should the returned product be determined to be hazardous All returns must be accompanied by a pre approved Returned Goods Authorization Number RGA Any return not received without a RGA may be disposed Contact Hamilton Customer Service at 1 888 525 2123 for an RGA Hamilton Precision Pipette Tips Hamilton delivers performance value and choice with two high quality tip families plus multiple formats and packaging options The tip families are low retention low binding tips for maximum sample recovery and standard tips for routine high throughput work Formats include standard non filter extended length hydrophobic filter barrier non sterile pre sterilized and macrovolume Packaging options include bulk bags hinged and lift off racks and stackable racks Hamilton tips are made from chemically resistant virgin polypropylene for excellent clarity and smooth surfaces All Hamilton pipette tips are certified to be free of RNase DNase and pyrogens Hamilton pipette tips are universal fit For more information visit ww
14. w hamiltonpipettes com 10 of 12 888 525 2123 2063 01 Rev E 2063 01 Rev E www hamiltonpipettes com 11 of 12 www hamiltonpipettes com Sales Support USA 1 888 525 2123 TRADEMARKS The following are trademarks of Hamilton Company AdvanTip Pipette Safe SoftGrip SofTouch Soffop SoftAide CE Ivo 12 of 12 Document No 2063 01 Rev E Hamilton Company 10 07 Printed in U S A
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