Hardware User`s Manual Passive avoidance box
Contents
1. There are 2 experimental cage models one for rats and one for mice The control unit can work with either one by simply changing a selector on the rear panel The position of the animal is detected using load cells installed below the platform Each tray that contains the grid has four legs that transmit weight to the load cell The system will not change side detection until the animal has moved completely to the other side The experiment can be controlled using e Computer The computer can control up to 8 cages using Shutavoid software and connecting the control units to serial port e Programmer The LE 2708 programmer can control 1 cage Passive avoidance box Panlab Sala 7 EQUIPMENT DESCRIPTION 7 1 CONTROL UNIT FRONT PANEL READY STATUS DOOR e Ready PASSIVE AVOIDANCE CONTROL LE 800 P Panlab Figure 6 Control unit front panel e READY Green led When the LE 800 control unit is turned on it will flash for 20 seconds indicating that system is auto calibrating After this period it will remain on continuously indicating that system is ready to work e STATUS Schematic diagram of the cage with four leds that show the status of the cage door open closed and animal position o Animal position left 1 and right 3 Two white leds o Door closed 2 Two small red leds that remain on when door is closed Figure 7 Box status leds e DOOR This button is used to test the door that separat2. 26 Shocker It sends the signal to activate the shocker e RAT MOUSE Selector to set the model of cage being used rat or mouse e POWER Power inlet main switch and fuse holder Passive avoidance box 10 Panlab pb de 7 3 EXPERIMENTATION CAGE WHITE SIDE DOOR MOTOR BLACK SIDE PLATFORM TRAY GRID TRAY GRID Figure 9 Experimentation cage front view e WHITE SIDE The left side is bigger and white It has a grid in the floor but shock can not be applied with it e BLACK SIDE The right side is smaller and black It has a grid in the floor which can apply shock to the animal e DOOR MOTOR There is a door between the white and black sides that can be opened and closed It is controlled by a motor e PLATFORM The platform is the base of the experimentation cage There are 2 load cells one for each side below the platform e TRAY Each side has a tray that holds the grid and collects animal excrements The trays can be removed to clean the cage They have 4 legs to transmit weight to the load cell e GRID Each side has a grid that is set in the tray Only the right side grid black side of cage can apply shock to the animal Passive avoidance box 11 Panlab H vara GRID DOOR BOX SHOCK Figure 10 Experimentation cage rear view There is a central box containing all the connections on the rear panel of the experimentation cage e GRID CONNECTOR DIN 6 female connector that transmits shock to
3. o Ambiental Ap s a implementac o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribui o para a do ambiente Passive avoidance box 27
4. ANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified type when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment check fuses according to the following procedure 1 Remove power cord from the power inlet Passive avoidance box Harvard Apparatus 2 Open fuse holder by pulling the flange with a regular screwdriver 3 Extract fuse holder using the screwdriver Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert again fuse holder both possible positions are correct because power supply is univ
5. Hardware User s Manual Passive avoidance box References L E870 76 0199 LE872 76 0200 Version V24 10 2014 T3 Panlab s l u International Calls 34 934 750 697 Pan a b H Horvord C Energia 112 Domestic Call 934 190 709 A isi 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info panlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab Sala 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be A exposed to a hazard Protection terminal ground connection Warning about a hot surface which temperature may exceed 65 C AN Warning about a metal surface that can supply electrical shock when it s A touched Decontamination of equipments prior to disposal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEEE A 2 GOOD LABORAT
6. ORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Passive avoidance box Panlab Sala 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original packing has been especially de
7. almente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de aparatos usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente lt y Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique 5 Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektris
8. before placing any animals on the grid period necessary for the system to reach a permanent thermal state 10 1 GRIDS CLEANING In the Passive Avoidance cage only the black side grid is prepared to supply electrical shock to the animal however you should clean both grids in order to keep the cage in good conditions to work When there is dirt in the grid the electrical shock flows through it This will produce erroneous data during the experiment because the animal behaviour to the shock negative reinforcement will not be correct it will not receive punishment In order to clean the grids you can use water and soap and then you must dry them Grids sest can be removed from the trays in order to clean them Be sure to dry well the DBg connector otherwise contacts may rust with the time Special care must be taken in cleaning the plastic between bars because urine is a good electrical conductor and current flow through it 10 2 TRAYS CLEANING Trays contains the grids and collet faeces and urine they should be cleaned after each experiment in order to clean them you should remove them from the cage and then you can use a soapy solution and finally dry them 10 3 WALLS CLEANING To clean the walls you can use a lightly wet cloth and then dry them with a dry cloth If they re too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth Passive avoida
9. che und elektronische Ger te durfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt p Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente 5 Nota Protecc
10. ed please read the LE 2708 Quick Procedure The necessary connections are described in the following table FROM CABLE LE 800 BOX CAGE BOX DB25 cable LE 2708 SHOCK CAGE SHOCK DIN6 cable MOTOR CAGE DBo cable GRID black CAGE DIN6 to DBa flat cable LE 2708 CAGE LE 800 LE 2708 DB15 cable Passive avoidance box 17 Panlab Sala 9 WORKING WITH THE EQUIPMENT 9 1 PROCEDURE The following procedure must be followed to work with equipment 1 Connect all the cables see Chapter 8 of this manual 2 If you work with the program Shutavoid and several control units choose patas a different ID for each cage a 3 Connect the control unit to the AC network and turn it on E Wait a few seconds until the O Ready flash during auto calibration led remains continuously on It will The door can be tested using the button labelled c on the front panel 6 If you re working with the rat cage select Rat with the selector naa Mouse on the rear panel If you re working with the mice cage select s Mouse with the same selector Z 7 Place the animal in the cage 8 Follow the Shutavoid program or LE 2708 indications for the experiment with the animal read related manual depending on the equipment purchased 9 Remove the animal form the cage once the experiment is ended and clean grids and trays 10 Return to point number 7 to begin work with the next animal 11 Once the experiment has finished turn o
11. ersal 6 Ifthe fuses blow again unplug the equipment and contact technical service For electrical safety never open the equipment The power supply has dangerous voltages WARNING Passive avoidance box Panlab 5 TABLE OF CONTENTS 7 2 7 3 10 10 1 10 2 10 3 11 12 13 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION EQUIPMENT DESCRIPTION CONTROL UNIT FRONT PANEL CONTROL UNIT REAR PANEL EXPERIMENTATION CAGE EQUIPMENT CONNECTION CONTROL WITH COMPUTER CONTROL WITH LE 2708 WORKING WITH THE EQUIPMENT PROCEDURE AUTOMATIC ERROR WARNING RECOMMENDATIONS GRIDS CLEANING TRAYS CLEANING WALLS CLEANING TROUBLESHOOTING PREVENTIVE MAINTENANCE TECHNICAL SPECIFICATIONS Passive avoidance box Harvard Apparatus 10 11 13 13 16 18 18 18 20 20 20 20 21 23 24 Panlab 6 INTRODUCTION The LE 800 Passive Avoidance Control is a system designed to conduct Passive Avoidance experiments The system is made up of a control unit and an experimentation cage The experimentation cage has 2 sides the left side is larger is white and does not have an electrical grid the right side is smaller is black and has an electrical grid to apply shock to the animal There is a door controlled by a motor between the two compartments Figure 5 Passive avoidance system
12. es the white and black sides of the experimentation cage Passive avoidance box Horvor Pa n la b on 7 2 CONTROL UNIT REAR PANEL BOX ID SHOCK RAT MOUSE POWER Main Remote 2708 Wo DEE TE Von Von C Rated Voltage 90 260 V AC 50 60 Hz 18VA Pain by Panlab s l MAIN REMOTE LE 2708 BOX Figure 8 Control unit rear panel e BOX ID Decimal selector used when the system is controlled by computer When several cages are connected to the same computer there can not be 2 cages with the same ID number e MAIN DBo female connector used to connect the LE 800 to the serial port of the computer When the computer controls several control units connected in cascade the serial port of the computer is connected to the MAIN port of the first control unit and the MAIN port of the next unit is connected to the REMOTE port of the previous control unit The REMOTE port of the last unit is left free e REMOTE DBo male connector used to connect the control unit to the MAIN port of the next control unit when several cages are controlled by a computer The REMOTE port of the last unit is left free e LE 2708 DB15 female connector used to connect the control unit to the LE 2708 programmer when control is not performed by computer e BOX DB25 female connector used to connect the control unit to the experimentation cage It transmits load cell signals and door activation signals e SHOCK Connects the LE with the LE 100
13. ff all equipment and clean the cage 9 2 AUTOMATIC ERROR WARNING The system checks if the cable between the rear panel connector labelled Box and the cage is connected Passive avoidance box 18 Harvard Pa n la b Apparatus If the system detects this error it will warn the user with the 2 flashing white position leds in the Status box on the front panel see Figure 7 The system will be awaiting the solution of the problem connect the cable Turn the system on again after solving the problem Once the cable is connected if the system detects that a load cell is damaged it will warn the user by flashing the white position led for this load cell see Figure 7 Passive avoidance box 19 Panlab Sala 10 RECOMMENDATIONS The experimentation cage must be periodically cleaned Trays and grids can be removed for cleaning Turn off the control unit and disconnect the flat cable that joins the cage with the black side shock grid prior to removing it 3 Once grids and trays are returned to the cage make sure that they are correctly installed the four legs of the trays must be in the load cell supports to correctly transmit weight to them 4 System auto calibration must be done without animals on the grid during auto calibration the system detects the grid and tray weight and balances them to detect animal weight 5 Although it is not strictly necessary it is recommended to wait 10 minutes after turning on the equipment
14. ity CONNECTOR LE2708 Pin Function 1 Door input 4 Shock Input 5 Right Output 6 Left Output 7 Change Output 9 5V 15 Gnd CONNECTOR BOX Pin Function 1 Door 2 Door GND 10 Transducer left S 11 Transducer left S 12 Transducer Right S 13 Transducer Right S 23 Transducer Exc 10V 24 Transducer Exc 25 Gnd Passive avoidance box 24 Panlab Als e DIMENSIONS Control Unit Width x Height x Depth 285mm x 7omm x 250mm Weight 1 84 kg DIMENSIONS Cage door C o al mm ET Passive avoidance box 25 Panlab Medis DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornella de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto Passive Avoidance Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 870 LE 872 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon le
15. nce box 20 Panlab Sala 11 TROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION e Check the condition of the fuses Ensure that the cable DB25 connects the cage and control unit Check that trays and grids are correctly placed If the cable is connected and all is correctly placed this means both load cells are damaged The two white position leds are blinking The load cell is damaged or One of the white position leds is blinking blocked Check that door cable is connected Check that DB25 cable is connected Check that Ready led is on if it s blinking system still is auto balancing and door will not work The door does not open Check that Ready led is on Check that grids and trays are correctly placed Check that the RAT MOUSE switch The control unit does not detect animal is in the correct position Rat position weight threshold detection is 3ogr if you work with mice the equipment will not be able to detect them Check that DIN6 cable is connected between the shock generator and cage Check that the DBg to DIN6 cable is connected between the grid and the cage Check that jack mono to green and black bananas is connected The animal does not receive shock Passive avoidance box 21 Panlab tower between control unit and LE100 26 Shocker Check that SHOCK CALIBRATION switch in the
16. orate the CE marking En cons quence ce produit peut incorporer le marquage CE En representaci n del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canalis _K General Manager Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 30 04 2014 Passive avoidance box 26 na Harvard Pa n la b H Apparatus Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment E P Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est leg
17. reserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Class A equipment is intended for use in an industrial environment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference N when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if WARNING not installed and used in accordance with these instructions may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Passive avoidance box Panlab Sala 4 MAINTEN
18. s directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mec nica Machinery directive Directive m canique Para su evaluaci n se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2013 Class A Safety of machinery EN ISO 12100 2010 This equipment complies with the limits for class A equipment in accordance with CISPR 11 definition and is classed as a Class A digital device pursuant to CFR Title 47 part 15 of the FCC Rules and is intended to be used in an industrial environment En consecuencia este producto puede incorporar el marcado CE Consequently this product can incorp
19. shock generator is in the SHOCK position Check that the INTENSITY knob in the shock generator is set to a value higher than omA Check that the grid is clean urine and excrements can conduct current Make sure your equipment is connected to PC via RS 232 Check that Shuitavoid settings so that the serial port is correct Restart the equipment and the PC The equipment does not communicate to do a RESET in communications with Shutavoid Check in the rear panel of control unit that the ID number is the same that the one set in Shutavoid Passive avoidance box 22 Panlab H 12 PREVENTIVE MAINTENANCE EXPERIMENT MONTHLY CHECK GRID AND TRAY PLACING Passive avoidance box Harvard Apparatus 23 Panlab H Sala 13 TECHNICAL SPECIFICATIONS POWER SUPPLY Input voltage Frequency Fuse Maximum Power Conducted Noise Universal input 100 Vac to 240 Vac 50 60 Hz 2 fuses 5x20mm 2 250V Fast 18 W EN55022 CISPR22 CISPR16 class ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 C to 40 C 0 to 85 RH non condensing o C to 50 C non condensing POSITION DETECTION Rat 30 gr Mouse 7 gr CONNECTOR MAIN REMOTE Pin Function 2 Rxd 3 Txd 5 Gnd 7 Rts 9 Cts COMUNICATIONS OUTPUT Standard Interface RS232C Connector Transmission speed Delta 9 contacts connector 19200 bauds 8 bits no par
20. signed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void ifimproper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits A For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category Il over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Passive avoidance box Panlab H PC Control Some of these instruments are designed to be controlled from a PC To p
21. ted vohage 90 260 Y AC 50 80 Hz 18VA Made in Spain by Pan ab s Figure 12 Example Control of 3 cages with same computer Passive avoidance box 14 Harvard Pa n la b Apparatus Some important points must be followed when working with more than one cage e Allthe units must have different IDs so that the computer can identify them e It is not necessary for all ID numbers to be correlative For example it is as correct to work with cages 1 2 and 3 as it is to work with cages 1 5 and 7 e It is not necessary for cages to be physically located in the order of their ID numbers For example it will be correct to work with cages 1 2 3 1 3 2 2 1 3 2 3 1 3 1 2 Or 3 2 1 e The computer serial port is always connected to the MAIN port of the first control unit e The REMOTE port of each control unit is always connected to the MAIN port of the next control unit e The REMOTE port of the last control unit is left free Passive avoidance box 15 Panlab 8 2 CONTROL WITH LE 2708 The LE 2708 Programmer can only control one passive avoidance cage Th necessary connections are shown in the following schematic End pulse Y O m c 3 Res pulse LJ Output 0 Figure 13 Control with LE 2708 Passive avoidance box 16 Harvard Pa n la b Apparatus The connections necessary to program the LE 2708 are not described in this manual If further information is requir
22. the right side grid black side e MOTOR CONECTOR DBg female connector at top side of the central box to control the door e BOX CONNECTOR DB25 male connector used to connect the experimentation cage with the box connector on the rear panel of the control unit e SHOCK CONNECTOR DIN 6 female connector used to connect experimentation cage to the LE 100 26 Shocker or the LE 2708 Programmer Passive avoidance box 12 Panlab Saro 8 EQUIPMENT CONNECTION 8 1 CONTROL WITH COMPUTER Box Id man Remote esse mm Made in Spain by Pandab s t na Figure 11 Connections schematic for control with computer Passive avoidance box 13 Panlab Harvard Apparatus The necessary connections when controlling passive avoidance with a computer are the following FROM TO CABLE GRID black CAGE DIN6 to DBg flat cable LE 800 MAIN PC SERIAL POIRT RS 232 cable LE 800 Shock LE 100 26 External 3 5mm mono jack to green Time and GND and black banana cable A computer can control up to 8 Passive Avoidance cages When working with several cages connection between control units and computer is the following an example of connection of 3 cages is shown in the Figure 12 Shock O Rat O Mouse Box LE 2708 Shock Mouse Box LE 2708 Shock O Rat Mouse LE 2708 Box ap B o gp j Ra
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