3 - Labmate (Asia)
Contents
1. Negative and positive selections can be executed simultaneously Sample volume from 250 uL to 8 5 mL per sample please visit www robosep com STRESS FREE CELL SEPARATION FOR YOU AND YOUR CELLS Why Choose RoboSep Reduce Hands On Time In a busy lab with samples arriving daily technician time is at a premium RoboSep can process up to 32 samples in an average working day with only 2 5 minutes of hands on time per isolation Eliminate Sample Contamination Some cell separation systems feature multi use columns and tubing increasing the risk of cross contamination RoboSep uses disposable tips in a column free system to prevent sample cross contamination Minimize Sample Handling Researchers who handle biological samples risk exposure to dangerous pathogens RoboSep fully automates all sample processing steps reducing potential risk CUSTOMER TESTIMONIALS KIM HENDERSON WORKS AT A LARGE CLINICAL LAB IN THE MIDWESTERN UNITED STATES AND WAS ONE OF THE FIRST RESEARCHERS TO PURCHASE A ROBOSEP UNIT We get up to eight samples a day that we sort for CD138 cells These cells are for different studies at the clinic and have to be processed the same day Using our old system it would take up to 30 minutes per sample because of the volume and we d have to be there all the time to load unload and clean the machine Now we can run four samples at once and have our enriched cells in an hour RoboSep us2. TECHNOL OO L E PRODUCT LISTING Human Negative Selection TYPICAL EASYSEP ROBOSEP FILTER EASYSEP CELL TYPE PERFORMANCE DATA REAGENT KIT TIP REAGENT KIT STARTER KIT T Cells Cb4 T Cells CD40 127 T Cell PBMC 9 95 93 WeENR TE CD8 T Cells B Coll NK Cells Plasmacytoid Dendritic Cel Basophils PMNC 92 99 19069 19069RF 19039 Hematopoietic Progenitor Cells 36 53 19056 19056RF 19046 M Hematopoietic Progenitor Cells Human Custom Enrichment Kit Isolate any human cell type by negative selection 19309 19309RF a m Contains any 3 standard enrichment kits Human Positive Selection TYPICAL EASYSEP ROBOSEP FILTER EASYSEP CELL TYPE PERFORMANCE DATA REAGENT KIT TIP REAGENT KIT STARTER KIT TISSUE PURITY CATALOG CATALOG CATALOG PBMC 99 100 18051 18051RF 18041 PBMC 97 100 18052 18052RF 18042 18043 CD 18048 CD4 CD29 CD4 0D127 CD25 cDaoD127 cDaeacDas w s O 84 a Tope CorTcl 99 Wa JERRE Mobilized WB or PBMC 85 99 dd 84 99 Fresh CB 75 96 18096 18096RF IEEE e Dwswmecw a ee ww s mm pom A Wr Mamay 9 9 xe 99 PE Selection large LabeSden5dDCds 3833 Human Custom Selection Kt Isolate any human cell type by postive selection 1830 asor O B c M o oot me ee
3. 5 10 minutes The magnetic field pulls the cells to the walls of the tube No Columns 4 Collect cells accordingly Negative Selection Cells of interest are poured off untouched without having been labeled with antibodies or magnetic particles Positive Selection Desired cells are labeled with magnetic particles and thus remain in the magnet Unlike the magnetic particles used in other positive selection systems EasySep magnetic particles do not interfere with flow cytometry EasySep Magnet Catalog 18000 The Big Easy EasySep Magnet Catalog 18001 The EasySep Magnet is designed to hold one 12 x 75 mm 5 mL polystyrene tube to isolate up to 2 5 x 10 cells or up to 5 x 10 cells when isolating rare cells e g CD34 per separation The Big Easy EasySep Magnet is designed to hold one 17 x 100 mm 14 mL polystyrene tube to isolate up to 10 cells or up to 2 x 10 cells when isolating rare cells e g CD34 per separation STRESS FREE CELL SEPARATION FOR YOU AND YOUR CELLS Untouched Human Cells By Negative Selection EasySep is the ideal cell separation tool for the isolation of untouched human cells Our Human Cell Enrichment Kits have been proven to isolate highly purified cells that are immediately available for downstream assays EasySep Human CD4 T Cell Enrichment Kit Catalog 19052 Fast Easy and Gentle Isolate cells without columns or washes Hig h Pu rity
4. A D WP E Positive selection reagents are designed for use with commercially available magnetic positive cell selection columns Columns are available in five sizes optimal cell number column 0 1 diameter 10 cells 0 3 diameter 5 x 10 cells 0 5 diameter 108 cells 0 6 diameter 5 x 10 cells and 1 0 diameter 107 cells Optimum cell number column can be doubled when using ficolled cells Reagents Positive or Negative Selection e Human mouse rat non human primate amp more e Compatible with other immunomagnetic column based systems 0 5 Tesla magnet including manual and automated systems e Incorporate any mouse IgG monoclonal antibody to create a customized TAC cell separation reagent Use any biotinylated or PE conjugated antibody with anti biotin or anti PE TAC e FACS compatible magnetic nanoparticles e A wide selection of antibodies are available for custom separations Please contact us to discuss your needs See next pages for composition of human negative amp positive selection amp mouse negative selection standard cocktails as well as suggested combinations of antibodies for particular cell subsets Tetrameric Antibody Complexes E Y ZA StemS en Immunomagnetic Mouse Cell Selection Q StemSep Negative Selection Cocktails target unwanted cells em amp E Hematopoietic Stem Progenitor Cell Hematopoietic Progenitor Cocktail CD5 CD11b CD45R B220 TER119 Ly 6G
5. Cells cocktail plus cocktail plus one or more of IL 18ra CD124 IL 4R CCR4 T1 ST2 Memory Resting Memory Resting amp CD4 T Cell amp CD4 T Cell amp CD8 T Cell amp CD8 T Cell CD4 T cell CD4 T cell cocktail CD8 T cell cocktail CD8 T cell cocktail cocktail plus plus one or more of plus CD45RA plus one or more of CD45RA CD25 CD69 HLA DR CD25 CD69 HLA DR Antibodies in standard negative selection cocktails are shown in blue Antibodies in standard positive selection cocktails are shown in yellow Antibodies suggested for addition to a standard cocktail for the enrichment of a particular cell subset are shown in red custom cocktail Other Enrichment Cocktails Depletion Cocktails s Neutrophil Basophil MEI Suggested custom 2 xr je CD2 CD3 CD14 cocktail CD23 IgE CD15 CD16 Use polymorphonuclear CD19 CD24 PMN cell rich fraction CD34 CD36 CD45RA CD56 glycophorin A Epithelial Tumor Cell CD2 CD14 CD16 CD38 CD45 CD66b glycophorin A Human Cell Enrichment from Mouse Human Chimeras anti mouse CD45 TER119 CML De Bulking CD2 CD3 CD14 CD15 CD16 CD19 CD24 CD56 CD66b IgE glycophorin A e CD3 T Cell Depletion CD3 CD8 T Cell Depletion CD8 Breast Carcinoma Depletion 5E11 BRSTI Antibody Cocktails Q Li Non Human Negative Selection Columns Supplied in 10 column p
6. Each kit contains enough reagents to select desired cells from 10 total cells except for the CD34 Positive Selection Kit which can process up to 5 x 10 cells the CD25 rie t selection kit which can process up to 8 x 10 cells and the Plasmacytoid Dendritic Cell Kit which can process up to 2 x 10 cells Kits contain human Fc receptor blocker when necessary to prevent nonspecific binding 18056 18056RF 18046 1 RoboSep Filter Tip Reagent Kits contain an EasySep Selection Kit conveniently bundled 4 Combined purity data of EasySep and RoboSep kits when available with 1 2 boxes of RoboSep Filter Tip Racks and a 250 mL bottle of RoboSep Buffer 5 Recommended for use with CLL and other abnormal samples 2 EasySep Starter Kits contain 3 standard kits and an EasySep magnet Catalog 18000 6 Pre enrichment kit optimized for maximum cell recovery subsequent cell sorting may be required 3 BM bone marrow CB cord blood PBMC peripheral blood mononuclear cells 7 Combo Kit must contain at least 2 different enrichment selection kits PMNC polymorphonuclear cells WB whole blood 8 RosetteSep enriched Catalog 15062 pu E STEMCELL Kits for Whole Blood Samples TYPICAL PERFORMANCE DATA EASYSEP ROBOSEP FILTER TIP CELL TYPE REAGENT KIT REAGENT KIT Kits for HLA Analysis TYPICAL EASYSEP ROBOSEP FILTER TIPS CELL TYPE PERFORMANCE DATA REAGENT KIT REAGENT KIT TISSUE ve PURITY CATALOG CATALOG
7. Eosinophils Neutrophils Hematopoietic progenitors Mesenchymal progenitors Mammary cells Tumor cells and more SEPTEMBER 2010 SZ Fuge Advantages etic StemSep Fre ets Superior a eee Cell Labeling A high performance system in which cells are Lee Felis le amp bound to a magnetic column 200000 all species c A cell types e positive or negative selection NIANA AwSStemSep Immunomagnetic Negative or gt ALL SPECIES Cell Selection ALL CELL TYPES HUMAN RAT OR NON HUMAN PRIMATE Human rat or non human primate cells are crosslinked to magnetic dextran iron particles using TAC Tetrameric Antibody Complexes Figure 1 TAC are comprised of two mouse IgG monoclonal antibodies shown in red amp blue in Figure 1 held in tetrameric array by two rat anti mouse IgG monoclonal antibody molecules shown in yellow in Figure 1 One mouse antibody molecule recognizes the cell surface antigen and the other recognizes the dextran on the magnetic colloid nanoparticle The cell suspension is then passed through a high gradient column of stainless steel mesh which is placed in a magnet NEGATIVE SELECTION The magnetically labeled unwanted cells bind to the column while the unlabeled enriched cells pass through untouched leaving them ready for further study Figure 2 StemSep method for Negative Selection of cells Add cocktail of tetrameric antibody complexes incubate then add magnetic colloi
8. Gr 1 Neutrophils 7 4 Cece SO Double Negative T Cell y T Cell B Cell NK Cell Dendritic Cell Thymocyte CD11b CD45R B220 T Cell Cocktail D4 CD8 CD11b CD5 CD22 F4 80 CD2 CD3 CD19 T Cell Cocktail plus TER119 Ly 6G Gr 1 plus antibodies Ly 6G Gr 1 TER119 TERI19 Ly 6G Gr 1 nic antibodies against against eutrophils 7 4 NK Cells CD4 NK cells TCRaB CD8 CD25 CD4 T Cell CD8 CD11b CD45R B220 TER119 Ly 6G Gr 1 CD8 T Cell CD4 CD11b CD45R B220 NK cells DX5 TER119 Ly 6G Gr 1 Naive CD4 T Cell CD4 T Cell Cocktail plus antibodies against NK Cells CD44 Naive CD8 T Cell Q CD8 T Cell Cocktail plus antibodies against CD44 CD4 T Cell Cocktail plus antibodies against NK Cells CD62L CD8 T Cell Cocktail plus antibodies against CD62L Resting CD4 T Cell Resting CD8 T Cell CD4 T Cell Cocktail plus antibodies Q CD8 T Cell Cocktail plus antibodies against CD25 against CD25 amp Memory CD4 T Cell amp Memory CD8 T Cell Biotinylated antibodies in standard negative selection cocktails are shown in blue Biotinylated antibodies suggested for addition to a standard cocktail to enrich a particular cell subset are shown in red custom cocktail NAIN 7 StemS en Immunomagnetic W Ww Human Cell Selection StemSep Negative Selection Cocktails target unwanted cells Hematopoietic Stem Progenitor Cell Hematopoietic
9. POSITIVE NEGATIVE DEPLETION Glycophorin A Depletion PBMC 18352 18352RF Each kit contains enough reagents to process 60 mL of whole blood unless otherwise stated EasySep Reagent Kit contains a positive selection cocktail magnetic particles and a 10 mL bottle of EasySep RBC Lysis Buffer 10X concentrate RoboSep Filter Tip Reagent Kit contains an EasySep Selection Kit conveniently bundled with 1 2 boxes of RoboSep Filter Tip Racks and a 250 mL bottle of RoboSep Buffer Combined manual EasySep and RoboSep purity data For 30 mL of Buffy Coat For 75 mL of Whole Blood or 37 5 mL of Buffy Coat For up to 100 mL of Whole Blood Contains HetaSep in place of EasySep RBC Lysis Buffer 10X concentrate PBMC peripheral blood mononuclear cells WB whole blood EasySep RBC Lysis Buffer may be purchased separately 10X concentrate 100 mL Catalog 20120 DW UPS Or decns e rz PRODUCT LISTING Mouse Negative Selection TYPICAL EASYSEP ROBOSEP FILTER EASYSEP CELL TYPE PERFORMANCE DATA REAGENT KIT TIP REAGENT KIT STARTER KIT Onkos pen 78 8 S O T e S ae 30 o8 m m i 92 98 80 90 Neutrophils 19762 19762RF 19732 83 94 Hematopoietic Progenitor Cells 76 92 19756 19756HF 19746 me T 0 9 1 34 unwanted CD45 Mammary Stem Cells Mammary Gland 1 Stem Cell per 20 90 Sorted Cells 19757 SSS Sa SS Mouse Custom Enrichment Kit Isolate any mouse Cell type by negative selection 19
10. Start 18 CD4 Cells Enriched 97 CD4 Cells Get purities of up to 99 Untouched Cells Use cells immediately in functional studies 8 E 8 8 s 0 Starting with frozen mononuclear cells the CD4 T cell content of the enriched fraction typically ranges from 92 97 Products for Research In Infectious Diseases Isolate CD4 T cells 19052 CD8 T cells 19053 and monocytes 19059 with minimal sample handling Cancer Immunology Get high recovery of highly purified NK cells 19055 to study tumor immunosurveillance Allergy and Inflammation Isolate basophils 19069 eosinophils 19256 and neutrophils 19257 without activation or degranulation Autoimmunity Isolate functional and viable regulatory T cells 15862 to study immune regulation and suppression For more products including our wide range of Human Positive Selection kits please see pages 13 15 Easy 50 EasySep Magnet Catalog 18002 EasySep Multistand Catalog 18010 The Easy 50 EasySep Magnet is designed to hold one 30 x 115 mm Separate up to 4 samples at once using the EasySep Multistand SSTEMCELE 50 mL conical tube to isolate up to 4 x 10 cells by negative selection from The multistand is designed to hold whole blood leukapheresis products or up to 4 EasySep Magnets or 4 EasySep mouse splenocytes or 2 x 10 cells from The Big Easy EasySep Magnets PBMC per separation Mouse Cell Sep
11. and APC Selection Kits Mouse Negative Selection Kits contain enough reagents to enrich desired cells from 10 total cells Kits contain mouse Fc receptor blocker when necessary to prevent nonspecific binding Other Species Positive Selection Kits contain enough reagents to select desired cells from 10 total cells 1 RoboSep Filter Tip Reagent Kits contain an EasySep Selection Kit conveniently bundled with 5 Kits contain pre enrichment cocktail and two fluorescently labeled antibodies for subsequent cell sorting 1 2 boxes of RoboSep Filter Tip Racks and a 250 mL bottle of RoboSep Buffer 6 Combo Kit must contain at least 2 different enrichment selection kits 2 EasySep Starter Kits contain 3 standard kits and an EasySep magnet 18000 7 Labels 4 x 10 total cells 3 BM bone marrow WB whole blood 8 EasySep enriched Catalog 19772 4 Combined purity data of EasySep and RoboSep kits when available THE CELL EXPERTS WWW STEMCELL COM TOLL FREE PHONE 1 800667 0322 PHONE 1 604 877 0713 TOLL FREE FAX 1800 567 2899 FAX 1 604 877 0704 ORDERS STEMCELL COM INFO STEMCELL COM FOR FULL CONTACT DETAILS WORLDWIDE VISIT OUR WEBSITE FOR RESEARCH USE ONLY NOT FOR THERAPEUTIC OR DIAGNOSTIC USE CATALOG 28960 VERSION 4 0 1 Total Lymphocytes T cells CD4 T cells CD8 T cells T cell subsets B cells Plasma cells NK cells Lymphoid cells Myeloid cells Monocytes Dendritic cells Granulocytes Basophils
12. any conjugated antibody with EasySep PE Biotin FITC or APC Selection Kits to select or deplete your cells of interest Use Your Own Mouse IgG Antibody Use any mouse IgG anti cell antibody with the EasySep Do It Yourself Selection Kit to select or deplete your cells of interest Design Your Own Custom Cocktail Contact us at info stemcell com to discuss your specific cell separation requirements We have a wide range of antibodies which can be easily incorporated into a Custom Selection Kit to address your unique applications For a complete listing of EasySep products see pages 13 15 ES STEMCELL HNOLOGIE The Fully Automated Cell Separator RoboSep is the first instrument to offer true walk away automation of immunomagnetic cell separation by performing all EasySep cell labeling and magnetic separation steps Just load your samples and reagents and return to separated cells in as little as 25 minutes High Throughput Labels and separates up to 4 samples simultaneously Versatile Positive or negative selection of virtually any cell type from any species or source including whole blood Fast and Efficient Cells are immediately available for flow cytometry functional studies or downstream analysis Capacity Positive selection processes up to 8 x 10 total cells 4 samples of up to 2 x 10 cells each Negative selection processes up to 4 x 10 total cells 4 samples of up to 1 x 10 cells each
13. 49 2644 9512 0 Belgium 0800 78 786 Czech Republic 420 56 730 26 81 Denmark 44 20 7537 7565 Finland 4 44 20 7537 7565 France 33 0 4 76 04 75 30 Germany 02644 9512 0 Greece 30 231 032 2525 Iceland 4 44 20 7537 7565 India 91 11 2622 7801 Ireland 4 44 0 20 7537 7565 Israel 4 972 3 934 9922 Italy 02 89139 545 Japan 03 3593 3211 Korea 02 3471 6500 Luxembourg 33 4 76 04 75 30 Netherlands 0800 023 2189 New Zealand 64 9 480 7775 Norway 44 20 7537 7565 Poland 71 349 12 46 Portugal 33 4 76 04 75 30 P R China 86 10 8261 2607 Singapore 65 9489 7967 Slovenia 33 4 76 04 75 30 South Africa 27 0 21 981 1560 Spain 093 446 4713 Sweden 44 20 7537 7565 Switzerland 61 712 11 15 Taiwan 02 2365 6266 Turkey 90 216 327 12 35 United Kingdom 44 0 20 7537 7565 6500 w HosetteSep PRODUCT LISTING Human Negative Selection CELL ENRICHMENT CATALOG TO LABEL 40 ML TO LABEL 200 ML COCKTAILS o Kejo p o hele gt CD4 CD25 Regulatory T Cells 1882 Total Lymphocytes 15263 Mesenchymal Stem Cells from Bone 15128 15168 Marrow Multiple Myeloma B and Plasma Cells 15129 15169 from Bone Marrow Circulating Epithelial Tumor Cells 15127 15167 Extensive Enrichment Circulating Epithelial Tumor Cells CD45 Depletion MONEE mite Any Cells Custom 15309 Le TO LABEL 40 ML TO LABEL 200 ML CELL DEPLETION COCKTAILS OSREE pede Peotone TO LABEL 250 ML TO LABEL 1000
14. 709 19709RF SS ss e iB Contains any 3 standard enrichment kits EasySep Combo Kit and an EasySep Magnet 18000 a a bit Mouse Positive Selection TYPICAL EASYSEP ROBOSEP FILTER EASYSEP CELE TYPE PERFORMANCE DATA REAGENT KIT TIP REAGENT KIT STARTER KIT On a MM CD4 CD25 Largo Spe 8 9 iw o Opa Tools oS MEE 0 0 oc CD90 Thy 1 2 Cere 8M 8 85 3EN iWSRF EA BM CD93 AA4 1 18762 18762RF 18732 SCA1 87 97 18756 18756RF 18746 i BM EOC Mmmm meer S PE Selection Larg Labes5x10 cels 864 Mouse Custom Selecion Kit Isolate any mouse cel ype by positive selection 18 00 BR o oO T Contains any 3 standard selection kits Eu au and an EasySep Magnet 18000 EIE E Other Species Positive Selection EASYSEP ROBOSEP FILTER EASYSEP PRODUCT NAME DESCRIPTION REAGENT KIT TIP REAGENT KIT STARTER KIT It U Do It Yourself Selection Kit se your own mouse IgG antibody 18098 18098RF 18498 Rat Custom Selection Kit Isolate any rat cell type by positive selection 19609 r j Rhesus Custom Selection Kit Isolate any non human primate cell type by positive selection 19809 Coo a ooo o s OE Mouse Positive Selection Kits contain enough reagents to select desired cells from 2 x 10 total cells with the exception of the CD25 gt s Kit CD4 CD25 Regulatory T cell Kits and PE FITC Biotin
15. Additional One Year Warranty Stores and reports user ID sample ID and reagent lot numbers MAEM eNeD IVO MNTSNSUNG e Software and procedures can be updated and usage data One Year Preventative Maintenance saved through the Ethernet or USB connections suse mess gaa e Laboratory automation can be controlled externally using WEN GEM i rautic ottle XML RPC for integration into automated processes sepe dli RoboSep Buffer 250 mL Technical Specifications RoboSep Buffer 5X Concentrate 250 mL Dimensions RoboSep Filter Tip Racks 1 box of 8 racks 1 1 L H 7 H Height with lid 56 cm 21 7 EasySep RBC Lysis Buffer 10X Concentrate 100 mL 2 Width 71 cm 27 74 Weight 26 kg 57 Ib Ethernet Cross Over Cable Power Requirements RoboSep Carousel e 90 60 Hz AC 100 240V The Big Easy EasySep Magnet e Connections RJ 45 10 100 Ethernet port 2 USB ports 1 1 2 boxes of RoboSep Tip Racks each containing 8 individual racks and 1 bottle of RoboSep Buffer are included with every purchase of a RoboSep Reagent Kit Filter Tip Racks can be obtained by ordering a RoboSep Filter Tip Reagent Kit see pages 13 and 15 Conditions for Operation e Temperature 10 30 C 50 86 F RoboSep is not specified for use in a cold room 4 C 39 F e Humidity 20 85 non condensing 2 10 mL of 10X RBC Lysis Buffer is included with every purchase of an EasySep or RoboSep Whole Blood Selection Kit see page 14 EISTEMCE
16. CELL SEPARATION Stress Free Cell Separation F r Yo and You r Cells Total Lymphocytes T cells CD4 T cells CD8 T cells T cell subsets B cells Plasma cells NK cells Lymphoid cells Myeloid cells Monocytes Dendritic cells Granulocytes Basophils Eosinophils Neutrophils Hematopoietic progenitors Mesenchymal progenitors Mammary cells S E MI C E Tumor cells and more Table of Contents The EasySep System How EasySep Works 3 4 5 Untouched Human Cells by Negative Selection 6 Mouse Cell Separation Customized Selection of Virtually Any Cell Type from Any Species 8 RoboSep the Fully Automated Cell Separator 9 Why Choose RoboSep 10 How RoboSep Works 11 Applications 13 Product Listings Fast and easy cell separation TECHNOLOGIES STRESS FREE CELL SEPARATION FOR YOU AND YOUR CELLS The EasySep System EasySep is a powerful immunomagnetic cell separation platform that combines the specificity of monoclonal antibodies with the simplicity of a column free magnetic system for the isolation of highly purified cells Fast and Easy Cells are ready in as little as 20 minutes with no columns or washes High Cell Purities and Recoveries Purities of up to 9926 with high recovery can be achieved Functional Viable Cells Gentle EasySep cell separations allow for functional and biological studies Extremely Versatile Perform positive or negative selection of virtually any ce
17. COM A S M F L IN NORTH AMERICA TOLL FREE T 1800667 0322 TOLL FREE F 1 800 567 2899 TECHNOLOGIES T 1604 877 0713 F 16048770704 E INFO STEMCELL COM IN EUROPE T 33 0 4 76047530 F 33 0 4 76 18 99 63 E IN AUSTRALIA TOLL FREE T F 1 800 060 350 T 61 0 7 5474 5042 INFO EUGSTEMCELL COM E INFO AUSGSTEMCELL COM 0500 gt WHAT IS i RosetteSep RosetteSep is a rapid cell separation procedure for the isolation of highly purified cells directly from human whole blood Figure 1 RosetteSep turns a simple density centrifugation step into a specific antibody mediated cell enrichment procedure with STEMCELL s tetrameric antibody complex TAC technology Figure 3 The RosetteSep cocktail crosslinks unwanted cells to multiple red blood cells already present in the sample forming immunorosettes Figure 2 amp 3 When centrifuged over the appropriate density medium e g Ficoll the unwanted rosetted cells pellet along with the red blood cells leaving the desired cells untouched and highly enriched at the density medium plasma interface Figure 1 Desired cells are never labeled with antibody and are immediately ready for any application FIGURE 2 Picture of a Blood Sample After Addition of the RosetteSep Cocktail and Prior to Centrifugation Over Ficoll Magnification 400X Label vete an cell FIGURE 1 RosetteSep Procedure 1 Add RosetteSep cocktail to whole blood Unwanted c
18. E TETRAMERIC ANTIBODY Eosinophils 14166 COMPLEXES TAC 14151 Catalog Numbers 13530 1 mL for labeling 5 x 10 cells Granulocytes 14161 13550 2 mL for labeling 1 x 10 cells aT 14157 13560 10 mL for labeling 5 x 10 cells pithelial Tumor Cells 14162 all sizes 12031 12032 5 x 10 cells 12051 12052 1x 10 cells 12061 12062 0 6 5 x 10 cells m m When using Ficolled cells optimum cell number per column can be doubled Note Not to be used for positive selection 0 1 0 3 green blue black green blue black Number of Simultaneous Separations Pump or Magnets and Other Equipment Gravity Description Feed 11030 red magnet gravity 1 and stand or pump 11050 green magnet gravity 1 and stand or pump 11060 blue magnet pump lto4 and stand 11070 black magnet pump 1 and stand T dq For a complete list of StemSep support products please see our catalog or website lt www stemcell com gt Catalog Number 0 1 0 3 0 5 0 6 0 3 0 5 0 6 0 3 0 5 0 6 1 0 0 1 0 3 05 0 6 1 0 VALVE temSep Advantage TACt Flexibility Unlike other immunomagnetic cell separation systems StemSep does not require coupling the anti cell antibodies directly to magnetic particles Instead StemSep utilizes flexible TAC technology The colloidal paramagnetic nanoparticles used in StemSep are coated with dextran allowing them to b
19. ML HLA COCKTAILS gt Re B 0 gt ROO B omphodevchment TTA LI TMA ATALOG SUPPORT REAGENTS 2 100 ML 500 ML LC NE NN NEN LC NN NN NEN L NENNEN NNNM Can be added to a standard RosetteSep cocktail if not already present Kit contains the required density medium Monocytes RosetteSep CUSTOM COCKTAILS CATALOG 15309 In addition to our standard RosetteSep cocktails custom cocktails may be easily prepared to meet your unique cell separation needs RosetteSep custom cocktails have been used by researchers to enrich various cell subsets such as T cells naive memory or resting T cells dendritic cells etc Anti Human Antibodies Available For Use in Custom Cocktails CD2 CD3 CD4 CD5 CD8 CD10 CD11b CD14 CD15 CD16 CD19 CD20 CD24 CD25 CD27 CD29 CD33 CD34 CD36 CD38 CD41 CD45 CD45RA CD45RO CD56 CD66b CD66e CD69 CD124 HLA DR IgE breast carcinoma and TCR Other antibodies are available upon request RosetteSep is an extremely flexible system which can also be used with your own in house IgG mouse monoclonal antibodies Design your own custom cocktails or contact us for assistance in optimizing your cell separations SELECTED RosetteSep PUBLICATIONS WITH REPORTED PURITIES WHERE AVAILABLE 1 Vivier et al Blood 111 5008 5016 2008 NK cell enrichment for functional studies 2 Neilet al Nature 451 425 430 2008 CD4 T cell enrichment for functional HIV stu
20. Progenitor Cocktail Primitive Hematopoietic f Progenitor Cocktail CD2 CD3 CD14 CD16 CD19 CD24 CD36 CD38 CD45RA CD56 CD66b glycophorin A CD34 Lymphoid Progenitor amp T Cell amp B Cell B NK Cell Dendritic Cell CD14 CD16 CD2 CD3 CD14 tees CD3 CD4 CD14 CD3 CD14 CD16 CD19 CD56 CD16 CD56 CD19 CD66b CD19 CD34 CD56 glycophorin A glycophorin A glycophorin A CD66b glycophorin A CD3 CD19 CD56 CD2 CD3 CD19 StemSep Positive Selection Cocktails target desired cells CD2 CD3 CD14 CD16 CD19 CD24 CD56 CD66b glycophorin A Myeloid Progenitor Monocyte Granulocyte CD2 CD3 CD14 CD56 CD66b CD19 CD56 glycophorin A glycophorin A CD14 45 Eosinophil W cD2 CD14 CD16 amp y T Cell amp a T Cell n CD19 CD56 ST cell cocktail plus T cell cocktail plus glycophorin A TCRaB TCRy Use polymorphonuclear PMN cell rich fraction amp CD4 T Cell amp CD8 T Cell CD8 CD14 CD16 CD19 CD4 CD14 CD16 CD19 CD4 CD25 CD56 glycophorin A CD56 glycophorin A amp Refer to CD4 CD8 RosetteSep amp EasySep tocol n amp Naive CD4 T Cell amp Naive CD8 T Cell CD8 CD14 CD16 CD19 CD8 T cell CD45RO CD56 cocktail plus antigen stimulation Y glycophorin A antigen Y CD45RO Effector CD4 T Cells stimulation Thl Th2 CD4 T cell CD4 T cell Effector CD8 T
21. acks with the exception of the 1 0 column which is sold individually Catalog Number Will Fit Magnet Size Column Diameter Optimum Cell Number per Column Gravity 12021 red green Cocktail Human Human Mouse Rat Primate to Enrich Negative Positive Negative Negative Cell T Selecti Selecti Selecti Selecti Aegae ell Type election election election election Seeon 5 x 10 cells 0 1 mL 5x 105 cells 1 mL for labeling dd n a ae w Eam 1 x 10 cells 0 2 mt 1x 10 cells 2 mL Ex w eE amm 5 x 10 cells 10 mL 2 x 10 cells 1 mt BOO cells C Sb We cele aun 5 x 10 cells 10 mL 14051 16151 T cells 14061 14751 16161 14052 16152 CD4 T cells 14062 14752 16162 14155 n 14053 16153 CD8 T cells 14063 14753 16163 14054 B cells 14064 14754 NK cells 14055 14755 14065 S 14153 Hematopoietic 14056 14756 Progenitors 14066 Primitive Hematopoietic 14057 Progenitors 14067 14058 Custom 14309 14709 13309 16309 16109 leti 14651 16251 T Cell Depletion 14661 16261 CD4 Depletion o EL y LL a 14652 CD8 Depletion 1 Seas Care mema more OPTIONS FOR ALL SPECIES Cell Depletion 14663 14154 CML De Bulking 14164 ANTI BIOTIN TETRAMERIC ANTIBODY COMPLEXES TAC Catalog Numbers 13030 1 mL for labeling 5 x 10 cells Human Cells from 13058 13050 2 mL for labeling 1 x 10 cells HOTS SUITED CHISTES d 13060 10 mL for labeling 5 x 10 cells B hi 14059 asophils 14069 i 14156 ANTI R PHYCOERYTHRIN P
22. aration EasySep is available in a wide range of kits to isolate mouse cells from any source including bone marrow lymph nodes spleen and whole blood EasySep Mouse Negative Enrichment Kits give you untouched viable cells which are ideal for in vivo ex vivo and adoptive transfer studies Negative selection of mouse cells uses biotinylated antibodies to target unwanted cells while positive selection uses PE conjugated antibodies to select cells of interest Easy and Gentle Isolate cells without columns or washes Convenient Use isolated cells immediately in downstream applications Q 2 3 Add biotin negative Add EasySep Biotin Selection Add EasySep selection or PE positive Cocktail negative selection magnetic particles selection labeled antibodies or EasySep PE Selection to a single cell suspension Cocktail positive selection a o T d Incubate Cell Suspension Biotinylated Antibody Anti Anti Biotin Dextran gt 10 Tetrameric Antibody Complex TAC VET lat fe Particle Incubate Schematic Drawing of EasySep Magnetic Labeling of Mouse Cells for Negative Selection Unwanted cells are specifically labeled with magnetic particles using biotinylated antibodies against cell surface antigens expressed on the unwanted cells and bispecific Tetrameric Antibody Complexes TAC These complexes recognize both the surface of the magnetic p
23. aration with a StemSep 0 1 or 0 3 negative selection column Depending on the column size a range of 10 8 x 10 cells can be loaded per column Green Magnet with Stand This device is designed to perform one gravity or Wy rnm Blue Magnet with Stand and Pump This device is designed to perform up to four simultaneous separations with the following StemSep negative selection column sizes 0 3 0 5 and 0 6 Depending on the column size a range of 2 x 10 1 5 x 10 cells can be loaded per column This magnet and stand can accommodate only pump feed columns Black Magnet with Stand and Pump This device is designed to perform a single pump pump feed separation with the following StemSep feed separation with a 1 0 bulk column Optimally H H 1 JJ JJ JJ C LX E negative selection column sizes 0 1 0 3 0 5 and 808000 10 cells can be processed with this column This 0 6 Depending on the column size a range of 10 E c a magnet and stand can also accommodate the 1 5 x 10 cells can be loaded per column E Y e following pump feed columns 0 3 0 5 0 6 and 1 0 giving a range of 2 x 10 1 5 x 10 cells per separation i e High gradient magnetic columns comprised of stainless steel mesh e High capacity columns designed for negative selection JJ i A e Pump P or gravity G feed M e Cell range from 10 to 1 5 x 10 cells depending on the column
24. articles and biotin Incubate High Purity Get purities of up to 99 4 Place tube in magnet for 2 5 10 minutes Collect cells accordingly Negative Selection desired cells are poured off into a new tube Positive Selection desired cells remain in tube Kits are available for the positive selection of mouse cells which provide highly purified labeled cells that are immediately available for flow cytometry Please see page 15 STRESS FREE CELL SEPARATION FOR YOU AND YOUR CELLS Customized Selection Of Virtually Any Cell Type from Any Species Highly purified cells from any species can be obtained by targeting the cells of interest with monoclonal antibodies to specific cell surface antigens Use your own biotin PE APC or FITC conjugated antibody or any mouse IgG antibody directed against a cell surface antigen on your cell of interest The labeled cell suspension is then mixed with the appropriate EasySep selection reagent and the standard EasySep procedure is followed 9 2 3 O Add your own antibody to Add EasySep Add EasySep Place tube in magnet a single cell suspension selection cocktail magnetic particles for 5 minutes e e m 2 Incubate Incubate Incubate Collect cells accordingly Cell Suspension f Negative Selection desired cells are poured off into a new tube Positive Selection desired cells remain in tube Use Your Own Labeled Antibody Use
25. d and incubate Unwanted cells are labeled Desired cells are unlabeled Pass cell suspension through column placed in magnet A Unwanted cells labeled bind to column Collect enriched cells Recovered cells have never been labeled with antibody and are ready for future studies Figure 1 Schematic drawing of StemSep TAC magnetic labeling of human rat or non human primate cells ns i Tetrameric Antibody Complex Magnetic Colloid POSITIVE SELECTION The magnetically labeled cells of interest bind to the column while the unlabeled cells pass through Cells of interest are collected from the positive selection column according to manufacturer s instructions The extremely tiny magnetic nanoparticles are com pletely FACS compatible and thus do not interfere with further studies Figure 3 StemSep method for Positive Selection of cells Add StemSep positive selection N cocktail incubate then add magnetic colloid and incubate directions recommended by positive Cells are ready for separation Follow selection column manufacturer x Cells of interest bind to column Unwanted cells pass through D FOR ANY SPECIES TRY A CUSTOM COCKTAIL OR USE THE STEMSEP PE OR BIOTIN TAC WITH YOUR OWN ANTIBODIES MouSE Mouse cells are magnetically labeled indirectly using a cocktail of biotinylated antibodies direct ed against cell surface markers on the unwant ed ce
26. dies Purity gt 90 3 Cobb et al Proc Natl Acad Sci USA 102 4801 4806 2005 T cell and monocyte enrichment for microarray analysis Purity 80 95 4 Rouhianinen et al Blood 104 1174 1182 2004 Monocyte enrichment for cell migration and RT PCR studies 5 Sasaki et a Blood 105 1204 1213 2005 CD4 T cell enrichment for microarray analysis Purity gt 90 6 Kohler et al J Leuko Biol 73 407 416 2003 Monocyte enrichment for culture and differentiation into macrophages 7 Dyugovskaya et al Am J Respir Crit Care Med 168 242 249 2003 yd T cell enrichment for functional and molecular studies 8 Poggi et a J Immunol 174 2653 2660 2005 NK cell enrichment for functional and molecular studies 9 Frelin et a Blood 105 804 811 2005 Hematopoietic progenitor cell enrichment for cytotoxicity studies 10 Castriconi et a Proc Natl Acad Sci USA 101 12640 12645 2004 Neuroblastoma cell enrichment from bone marrow for molecular characterization studies NK cell enrichment for functional studies 11 Parsons et al Blood 104 2736 2738 2004 Mesenchymal stem cell enrichment from fetal bone marrow for culture and virus susceptibility studies Purity gt 99 after 4 days culture 12 Vasir et al J Immunol 174 2376 2386 2005 T cell and B cell enrichment for cytokine activation studies Purity 95 9796 T cell gt 90 B cells SELECTED ASHI ABSTRACTS FOR HLA ANALYSIS 1 Dunn DM Thurmon MN Sypert CL Cel
27. e bound by the anti dextran end of any StemSep TAC reagent Since the anti cell end of a bi specific TAC reagent can be varied TAC can be easily customized by incorporating any cell specific mouse IgG monoclonal antibody instantly creating a novel cell separation reagent If a mouse IgG monoclonal is not available for a particular cell surface antigen we provide anti biotin or anti PE TAC allowing the use of any biotinylated or PE conjugated cell specific antibodies in your separations This flexibility of StemSep and our custom cocktail program have allowed many researchers to perform unique cell separations that would otherwise not have been possible Tetrameric Antibody Complexes This product is available only as a custom order Please contact us for more information StemCell Technologies TECHNOLOGIES In North America Tel 1 604 877 0713 Fax 1 604 877 0704 Toll Free Tel 1 800 667 0322 Toll Free Fax 1 800 567 2899 e mail info stemcell com e mail orders stemcell com In the United Kingdom Tel 44 0 20 7537 7565 Fax 44 0 20 7515 5408 Toll Free within United Kingdom Tel 0800 731 27 14 Fax 0800 731 27 13 e mail info stemcellgb com www stemcellgb com In Europe Tel 33 0 4 76 04 75 30 Fax 33 0 4 76 18 99 63 e mail info stemcellfrance com www stemcell com Please contact us directly or one of our international distributors at Australia 61 3 9790 4100 Austria
28. ells are crosslinked to red blood cells rosetted with tetrameric antibody complexes Incubate 20 minutes at room temperature 2 Layer over density medium e g Ficoll FIGURE 3 Illustration Showing Rosette of Unwanted Cell and RBCs Ficoll Formed by RosetteSep Tetrameric Antibody Complexes nwanted b gt JA argeted for y lt Ps LT Plasma A f N Enriched cells Ma AF q Ficoll Red blood cells and unwanted cells rosetted ep 3 Collect Ask us about a St RosetteSep demonstration in your lab Enriched cells are left untouched unrosetted Ficoll and Ficoll Paque PLUS are trademarks of GE HealthCare Ltd POWERFUL CELL SEPARATION WIIH A y eee EXAMPLES OF i RosetteSep APPLICATIONS COCKTAILS CAN BE USED DIRECTLY WITH BD VACUTAINER CPT CELL PREPARATION TUBES BD Vacutainer CPT Cell Preparation Tubes are evacuated blood collection tubes containing a density gradient liquid below a gel insert and an anticoagulant above The RosetteSep procedure can be performed directly in the CPT minimizing sample manipulation The gel insert in the CPT keeps the sample and the density gradient layer apart before and after centrifugation avoiding the layering step and facilitating removal of the enriched cells To perform a RosetteSep separation in a CPT the RosetteS
29. ep cocktail is added to the blood sample directly in the CPT After incubation and centrifugation the enriched cells are recovered by pipetting or pouring from the CPT This approach may be advantageous when minimal sample handling is preferred such as when processing infectious samples Available from BD www vmdp com BD Vacutainer and CPT are trademarks of Becton Dickinson and Company ISOLATE SPECIFIC T CELL SUBSETS EXAMPLE REGULATORY CD4 CD25 T CELLS CATALOG 15862 To isolate CD4 CD25 T cells first enrich CD4 T cells with RosetteSep then select CDA CD25 T cells using EasySep CD25 Selection Cocktail immunomagnetic cell selection without columns Activated non CD4 T cells are removed during the RosetteSep procedure prior to CD25 positive selection For more information on EasySep please visit http www stemcell com product catalog easysep aspx WORKS WITH ANY RED BLOOD CELL CONTAINING SAMPLE RosetteSep can be used with samples other than whole blood With the appropriate procedure cells can be enriched from buffy coat bone marrow cord blood leukapheresis and spleen QUICK AND EASY GRANULOCYTE DEPLETION CATALOG 15624 HosetteSep9 Granulocyte Depletion reagent can be used to eliminate granulocytes from older blood samples SAFE HANDLING FOR HIV RESEARCH HIV research often requires cell isolation procedures that minimize sample handling RosetteSep is a safe and easy to use tool for t
30. es dedicated pipette tips for each sample so there s no issue with cross contamination Because the process is all automated we can get other things done in the meantime It makes our job a lot easier when we don t have to check the timer every ten minutes We re a busy lab and RoboSep really saves us a lot of time WENDY LEONG LAB MANAGER AT A PATHOLOGY BLOOD CENTER LABORATORY We like the reliability of the RoboSep the minimization elimination of specimen handling by the tech during subset separation and the low maintenance of the instrument These factors are important to us with such a high throughput of samples processed E sreMcELL TECHNO How RoboSep Works Select protocol Load carousel with e samples e EasySep Selection Cocktail e magnetic particles e buffer and tips 2 Press Run 3 Return in 25 to 60 minutes to collect your separated cells Applications STRESS FREE CELL SEPARATION FOR YOU AND YOUR CELLS RoboSep is the only fully automated cell separator that provides researchers with an efficient and easy to use option for the enrichment of cell populations for various downstream applications Whole Blood Processing Label and separate up to 4 5 mL of blood per sample HIV Minimize sample handling to reduce risk of exposure to dangerous pathogens HLA Labs Cells are immediately available for any downstream HLA application including serology flow c
31. he separation of whole blood with minimal sample handling that avoids cross contamination of samples and minimizes exposure to virus ISOLATE CELLS FOR MACROPHAGE RESEARCH CATALOG 15624 Cell separation with RosetteSep produces unlabelled cells that can be used in any application Monocytes isolated with RosetteSep Catalog 15028 are untouched and immediately ready to be differentiated into macrophages ISOLATE CELLS FOR HLA ANALYSIS After RosetteSep enrichment cells are immediately usable for serological HLA testing chimerism and flow crossmatch analysis Because RosetteSep does not interfere with subsequent analysis and is gentle on cells this procedure can reduce the risk of false positive data in HLA applications KITS FOR SPECIALIZED ROSETTESEP APPLICATIONS LYMPHOID amp MYELOID ENRICHMENT KITS FOR LINEAGE SPECIFIC CHIMERISM ANALYSIS CATALOG 15271HLA Lymphoid CATALOG 15272HLA Myeloid These kits for monitoring engraftment after transplants provide sufficient enrichment cocktail and specially designed density medium to perform 20 tests CORD BLOOD PROGENITOR ENRICHMENT KIT CATALOG 15276 This kit allows the routine enrichment of hematopoietic progenitors from large volumes of cord blood samples One kit can process up to 500 mL of cord blood
32. ired to load the instrument with samples and reagents and to select one of the pre programmed protocols Instrument Time is the length of time that RoboSep requires to complete the cell separation s 3 Some negative selection protocols require two quadrants of the RoboSep carousel only two such protocols can be performed simultaneously is Supplied With Mechanical Components e RoboSep Carousel Automated tip loading and ejection Hydraulic Fluid Bottle Robotic Dual tip head uses 1 mL and 5 mL tips One Year Warranty Arm Accurate pipetting range 12 5 uL to 10 mL RoboSep Accessory Kit Air displacement pipetting e 4 The Big Easy EasySep Magnets 16 Tip Racks in total e RoboSep Buffer 250 mL Can accommodate 4 The Big Easy EasySep magnets e RoboSep Service Rack Can accommodate up to 4 different samples e USB Keyboard e USB Mouse Reliable pump requires no user servicing e USB Memory Stick Uses distilled water as hydraulic fluid avoiding problems y Hydraulics associated with salt crystal formation Magnet Shields Easily accessible hydraulic fluid bottle with sufficient volume for e Tip Head Cleaners several days of operation e User Manual e RoboSep Timer Quick Start Sheet RoboSep amp Accessories User Interface M PRODUCTNAME CATALOG A RoboSep 20000 e Intuitive graphical user interface Integrated 8 color touch screen AAO S One Weak WATAY e Pre programmed protocols for EasySep selection kits
33. l preparation that can be used for serological HLA typing crossmatching and flow crossmatching ASHI Abstract 79 2003 2 Gautreaux MD Haywood MJ James CM White CD Evaluation of RosetteSep with modification of test protocol to increase yield and reduce red blood cell contamination ASHI Abstract 120 2003 3 Martin C McKie J Hazy L Shumway W LeFor W Purified T and B cell preparations obtained using RosetteSep cell isolation reagents suitability for use in cytotoxic assays ASHI Abstract 96 2002 CATALOG 28435 VERSION 3 0 0 AUGUST 2008 ONE STEP CELL ENRICHMENT DIRECTLY FROM WHOLE BLOOD ROSETTESEP FEATURES HIGH RECOVERY No post Ficoll cell losses NEGATIVE SELECTION Cells are unlabeled and ready for immediate use UNTOUCHED CELLS Yield high quality RNA and DNA for molecular studies FLEXIBLE Process large or small volumes and multiple samples simultaneously SAFE Minimal sample handling KITS AVAILABLE FOR T cells CD4 T cells CD8 T cells B cells Total lymphocytes Multiple myeloma cells Monocytes Progenitors Circulating epithelial cells Granulocytes Mesenchymal stem cells and more SIMPLE No Magnets No Columns FAST No Special Equipment Just a 20 minute EASY incubation at room temperature prior to a standard Ficoll M spin PLUS DEPLETION OF Granulocytes Monocytes IgE bearing cells T cells amp subsets WWW STEMCELL
34. ll type from any single cell suspension or species Any Sample Source Isolate cells directly from any sample source including whole blood Flow Cytometry Compatible Isolated cells are immediately available for downstream analysis Isolate Virtually Any Cell Type with Purities of up to 99 Including Total lymphocytes NK cells Hematopoietic progenitors T cells Lymphoid cells Mesenchymal progenitors CD4 T cells Myeloid cells Mammary cells CD8 T cells Monocytes Tumor cells T cell subsets Dendritic cells and more Regulatory T cells Granulocytes B cells Basophils B cell subsets Eosinophils Plasma cells Neutrophils EXSTEMCELL To see EasySep in action a 7 57 74 7 For a complete list of EasySep Products see page 13 15 TECHNOLOGIES How EasySep Works Add EasySep Add EasySep selection cocktail magnetic particles The EasySep cocktail targets cells for selection EasySep magnetic particles bind to or depletion positive or negative selection labeled cells using Tetrameric Antibody Complexes TAC The TAC contain monoclonal antibodies directed against specific cell surface antigens TEasySep D ami ar Incubate 10 15 minutes Incubate 5 minutes Cell Suspension y Ru Dextran EasySep kits contain MN a EasySep selection cocktail amp D Tetrameric Antibody EasySep magnetic particles 4 Complex TAC 3E Place tube in magnet for 2
35. lls Figure 4 Cells are then crosslinked to magnetic dextran iron particles using TAC Tetrameric Antibody Complexes TAC are com prised of two mouse IgG monoclonal antibodies held in tetrameric array by two rat anti mouse IgG monoclonal antibody molecules One mouse antibody molecule recognizes biotin and the other recognizes dextran on the magnetic nanoparticle The cell suspension is then passed through a high gradient column of stainless steel mesh Figure 5 The magnetically labeled unwanted cells bind to the column while the unlabeled enriched cells pass through Figure 4 Schematic drawing of StemSep TAC magnetic labeling of mouse cells Biotinylated Anti Anti D eI i Dextran 4 AND Cel en NEN ind Tetrameric Antibody otl Complex Magnetic Colloid NEGATIVE SELECTION Figure 5 StemSep method for Negative Selection of mouse cells RON 3 Add cocktail of oS biotinylated antibodies e p Unwanted cells are labeled 2 Desired cells are unlabeled 4 Addtetrameric antibody complexes incubate then add magnetic colloid and incubate Pass cell suspension through column placed in magnet Collect enriched cells Recovered cells have never been labeled with antibody and are ready for future studies B VY TX StemS5er Equipment amp Magnets Red Magnet with Stand This device is designed to perform one gravity or pump feed sep
36. ytometry crossmatch and chimerism analysis Chimerism Analysis Numerous selection strategies are available for sequential separation of multiple cell populations from a single patient sample Multiple Myeloma Isolate CD138 cells with high purity from bone marrow whole blood and PBMC for accurate and reliable diagnostics RoboSep fits into a standard biocontainment hood for sterile operation Molecular Diagnostic Studies Get highly purified cells for the extraction of high quality RNA and DNA Flow Cytometry Core Centers Reduce time required on the flow cytometer when sorting for rare populations by pre enriching cells Hematopoietic Stem and Progenitor Cells Enrich rare stem and progenitor cell populations using a CD34 positive selection or lineage depletion strategy Custom Protocols Use over 150 protocols specifically designed to select different cell populations or customize your own protocols for unique applications TECHNOLOGIES Fully Automated Cell Separation For Significant Time Savings dosis seem human 2 min 48 60 min 50 62 min P mouse 2 min 69 77 min 71 79 min coc mec cc human 2 min 20 48 min 27 50 min 9 mouse 2 min 30 74 min 32 76 min EE TE R human 5 min 64 78 min 69 83 min P mouse 5 min 86 103 min 91 108 min Dinan E human 5 min 20 51 min 36 56 min g mouse 5 min 41 77 min 46 82 min Hands On Time refers to the average length of set up time requ
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