Array View Troubleshooting
Contents
1. Run_s700 063_2002 06 06_25 gel Sf 00 063 Run_s700 063_2002 06 06_26 ge Sf 00 063 Run_s700 063 2002 06 06 _ 27 gel 3700 063 Run 3700 063 2002 08 06 28 qel ad File nare OK l l This window will display the entire run Files of type Entire Fun Display geh Cancel Spatial Troubleshooting Module Page 12 of 30 Applied Biosystems Entire Aun Display for 00RA Aundi 07 20_16 21 24 gel Two different oligomers one labeled with dROX Red and the other labeled with dR110 Blue are loaded to all 104 capillaries in a special pattern Caps 1 4 receive red dye Caps 5 100 receive blue and red alternating by capillary Caps 101 104 receive blue dye Scan umber 30 Capilar umber 107 Spatial Troubleshooting Module Page 13 of 30 BS App e ms Left to Right Variation Optimized spatial Note the uniformity across the array Although a peak is missing the software will extrapolate the position of this capillary Spatial Troubleshooting Module Page 14 of 30 Applied Biosystems Severe Left to Right Signal Variation Optimize the cuvette temperature in 3 C increments Temperature gradients in the cuvette cause deviation of the laser beam If a bubble is not present in the cuvette try modifying the method by changing the cuvette temperature in 3 C increments From the default temperature of 40 C initially try 37 C and 43 C cuvette temperature when running the2. can range from 35 to 50 C Refer to page 4 16 of the ABI Prism 3700 DNA Analyzer User Guide for instructions on optimizing the temperature A large group of capillaries have a very low signal Possible error messages Array is not seated properly Verify that the array loading end header is seated correctly not under Red Profile Error tension and the capillary tips are positioned in their injection wells Blue Profile Error Blue and Red Profile Error End Caps Blocked No Alignment Found Sample loading issue Verify that the fittings on the ports above the sample transfer syringes are Bubbles in the sample finger tight transfer lines Verify that the sample transfer syringes are tightened to the pump Finger tight should be sufficient Water lines feeding the Verify that the fluid line sinkers are at the bottom of the reservoir sample transfer pumps are not underwater in the water Verify water levels in the water reservoir Replenish if necessary reservoir Run the Change tips wizard This will prime the syringes and flush water through the tubing going to the autoloader tips During the wizard monitor both sample transfer syringes for bubbles If bubbles are present in the syringe but not the tubing from the water reservoir change the syringe Clogged autoloader tips Replace the autoloader tips After installation verify that the tips are tightened until finger tight Spatial Troubleshooting Module Page 9 of 3
3. 0 BN Biosystems A non functioning sample Refer to the Verifying Syringe Pump Module transfer pump Wide peaks No sheath flow the most Check the sheath flow syringe for leakage replace syringe if necessary common cause Possible software messages Check for leakage around the sheath flow syringe fittingsT Clean the area No Alignment Found with a lint free tissue moistened with DI water and tighten the fittings until Non Unique Alignment finger tight Refer to the Checking for Leaks Module Cap Spacing Out of Range The syringe pump may not be moving Refer to the Verifying Syringe Pump Module to troubleshoot If problem persists please contact technical support Contacting Technical Support By phone 1 800 831 6844 option 5 By email ABTechnicalSupport appliedbiosystems com Spatial Troubleshooting Module Page 10 of 30 Applied Biosystems The Array View The array view is an option in the Data Collection software which allows monitoring of the data during or after a run It is provides a quick overview of a run and is useful in identifying hardware related issues that can affect the samples Troubleshooting Module for more information See the Array View Colors How is the array view accessed in Data Collection V2 0 During a run 1 Select the Run Status tab gt select Array View Fie Caution Always exit the Array View when you are finished viewing Do not leave the window open for extended periods during a r
4. 15 24 47 on 113 Spatial Troubleshooting Module Page 17 of 30 Applied BS Biosystems Missing Peaks The spatial passed despite missing many red plumes because the spacing was correct and the spatial algorithm could guess the missing plume positions Although it passed this profile is indicative of a problem and the spatial should be rerun gt Spatial Calibration Profile bor Auni 38 08 04_ 10 49 23 on 013 Spatial Troubleshooting Module Page 18 of 30 Applied BS Biosystems Running service modules within Data Collection V2 0 ae Aun Service Modules Step 1 Go to the Instrument menu gt Select Utilities gt Select Run Service Module gt Step 2 Click the Select Module button z Er Data Collection Software Vertion 2 0 all b memari Hi j siiri Shp ore meen RISTE Spatial Troubleshooting Module Page 19 of 30 Service Module Location EJ Service Modules ea s L Look ir ea racleDE D _ AppliedBio _ Support Files Data Collection Support Service Modules ge Programs E amp IR Files of type All Files ee Cancel kl Ed c PopT ov aterE xchange 8 PostBatchInternall se Up One Level a PreBatchinternalll se0 nly mod a STA SynngeP rine mod Ej Ww aterF lush mod a Water oPopE change mod Service Module Location Look ir 3 AuboPlateD eck Calibration mod a BubbleRemove mod l CuvetteF l
5. Applied Biosystems 3700 Instrument Spatial Troubleshooting Module The purpose of this module is to guide the user through troubleshooting a spatial The software instructions are for Data Collection Software V2 0 BEFORE PERFORMING ANY TROUBLESHOOTING WORK ON YOUR 3700 INSTRUMENT PLEASE READ THE ABI PRISM 3700 DNA ANALYZER USER S MANUAL FOR SAFETY AND WARRANTY INFORMATION AND FURTHER DETAILS ON USE OF THE SYSTEM Please contact technical support if you have any questions regarding the document The use of Service Tools is advised only under the recommendation of an Applied Biosystems representative Checking the array for broken capillaries Before installing a new array it is recommended to check the array for broken capillaries the loading end the length of the array and the detection end Refer to the array diagram for specific areas If the array is broken please report the problem to technical support Preparing for a Spatial Before starting a spatial it is important to ensure that the instrument is running properly and the reagents are fresh Follow the checklist below Perform system maintenance Refer to the 3700 Instrument Maintenance Module Check for bubbles in the cuvette if present run the cuvette flush module Refer to the Checking CCD view module Spatial reagents should be fresh If reagent age is unknown or was stored improperly recommended storage is 2 8 C order a new Spatial calibration standard part
6. Spatial Calibration Running at both temperatures will help to determine if it is necessary to increase or decrease the temperature incrementally Continue to change the temperature incrementally to get an optimal spatial The temperature range should be from 35 C to 50 C do not go lower than 34 C as the instrument may error A high ambient temperature can prevent the instrument from reaching temperatures below 34 C Once the temperature is optimized run the spatial and all subsequent runs using this array with the optimized temperature Temperature gradient problems may show other possible profiles such as higher signal on the left than on the right or a smile pattern Spatial Calibration Profile for Run1999 11 18_10 10 06 on O1d13 24000 20000 16000 italy sat pay Aada ata uy Lill lil wih 1357911 14 17 20 23 26 29 32 35 38 41 44 47 50 53 56 59 62 65 68 71 7477 80 83 86 89 92 9598 102 Intensity vs Capillary Number o Example of a left right failure Spatial Troubleshooting Module Page 15 of 30 Applied BS Biosystems Set of Four Spatial Calibration Profile for Runt 939 07 12_15 02 053 on C63 ul Wi 0 i i Ml Set of Eight Irofile from SpatielCal SN3d6 Run 9 05 24 14 39 20 ecl ni ALA AAA ALL alt me Spatial Troubleshooting Module Page 16 of 30 Applied BS Biosystems Broad Peaks Spatial Calibeation Profile for Aun 999 406 272
7. aks Module if the bubbles in the cuvette persist Bright events are present in the Particles in the cuvette Inspect the tubing around the inline filter for leakage indicated by dried cuvette which are detected as false polymer If leaking is observed replace the inline filter peaks Run the CuvetteFlush mod service module Possible error messages Non Unique Alignment Capillary array may be Verify that the thumbscrews on the detection end base are not too tight and Cap Spacing Out of Range misaligned are screwed on with equal tightness Too Many Caps A few peaks have greater intensity Bubbles in the cuvette Run the Cuvette Flush module Refer to the Checking for Bubbles Module than the others and the Checking for Leaks Module if the bubbles in the cuvette persist Spatial Troubleshooting Module Page 8 of 30 Applied Biosystems Possible Causes Recommended Actions Inspect the tubing around the inline filter for leakage indicated by dried polymer If leaking is observed replace the inline filter Run the CuvetteFlush mod service module Capillaries on one side of the array Bubbles in the cuvette Run the Cuvette Flush module Refer to the Checking for Bubbles Module have no fluorescence and the Checking for Leaks Module if the bubbles in the cuvette persist or Presence of reflective particles in the cuvette Cuvette temperature needs optimization Optimize cuvette temperature in 3 C increments The temperature
8. al Troubleshooting Module Page 23 of 30 Applied Biosystems Array Diagram Load header Loading end Detection and Capillaries Sheath tip corer Loading end of array Broken capillaries along the length of the array can be problematic if the array is not changed Polymer will still flow through the capillary causing buildup of polymer in the area and affecting other capillaries Exposing the Detection end of the array Step 1 While holding the detection end base in front of you a Pull the tip cover clips laterally b Carefully slide the tip cover downwards to expose the tips and O ring IMPORTANT With the cover retracted the fragile ends of the capillaries are exposed Any trauma to the tips such as pressure from fingers during handling will cause the tips to break and make the array unusable The tips should appear uniform in height Before installing the array on the instrument return the tip cover to its original position Spatial Troubleshooting Module Page 24 of 30 NS Applied Ap Biosystems The capillary tips Do not touch the capillary tips as they are delicate and may break Thumbscrews MOON Apiary Arrow GENEN Thumbscrews These should be tightened until finger tight Spatial Troubleshooting Module Page 25 of 30 Applied Biosystems Sheath Flow syringe and pump The red arrows identify areas of possible leaks Port 8 From polymer bottle to syringe Port 9 From syringe to c
9. ble in one of the red sample Inspect the tubes with the red standard for bubbles in the bottom if oe tubes necessary centrifuge the sample tube yal illu l ile il Nh Array is not seated properly Verify that the array loading end header is seated correctly not under merrier tension and the capillary tips are positioned in their injection wells click to enlarge Sample loading issue Verify that the fittings on the ports above the sample transfer syringes are Bubbles in the lines of one of finger tight the sample transfer tips Verify that the sample transfer syringes are tightened to the pump Finger tight should be sufficient Verify that the fluid line sinkers are at the bottom of the reservoir Water lines feeding the sample transfer pumps are Verify water levels in the water reservoir Replenish if necessary not underwater in the water reservoir Run the Change tips wizard This will prime the syringes and flush water through the tubing going to the autoloader tips During the wizard monitor both sample transfer syringes for bubbles If bubbles are present in the syringe but not the tubing from the water reservoir change the syringe A clogged autoloader ti Replace the autoloader tips After installation verify that the tips are tightened until finger tight A non functioning sample Refer to the Verifying Syringe Pumps Module for more details transfer pump Part of the profile has spiky peaks Bubbles or particles in cu
10. ed before placing on the instrument Bubbles in the sample Verify that the fittings on the ports above the sample transfer syringes are transfer lines finger tight Verify that the sample transfer syringes are tightened to the pump Finger tight should be sufficient Water lines feeding the Verify that the fluid line sinkers are at the bottom of the reservoir sample transfer pumps are not underwater in the water Verify water levels in the water reservoir Replenish if necessary reservoir Run the Change tips wizard This will prime the syringes and flush water through the tubing going to the loading tips During the wizard monitor both sample transfer syringes for bubbles If bubbles are present in the syringe but not the tubing from the water reservoir change the syringe Clogged autoloader tips Replace the autoloader tips After installation verify that the tips are tightened until finger tight Electrophoresis Issues Refer to the Electrophoresis Troubleshooting Module A non functioning sample Refer to the Verifying Syringe Pumps Module for more details transfer pump Spatial Troubleshooting Module Page 6 of 30 Applied Biosystems Possible Causes Recommended Actions Prepare the 200uL of each color standard in a single tube vortex or pipette mix and then pipette 100uL of standard into 2 tubes for running This ensures that each tube is identical Spin down samples before placing on the instrument I
11. firmware and software memory problems we recommend that you restart the instrument and the software once a week To shut down and restart the instrument Step 1 The instrument should not be running or extracting data Step 2 Close the 3700 Data Collection software by selecting Shutdown from the File menu Note You cannot use the Close button to exit the software Step 3 Close the OrbixWeb Daemon software by right clicking on its button in the taskbar and selecting Close from the pop up menu If you get a run time message click OK to close the message IMPORTANT Do not shut down the OrbixWeb Daemon until after you have shut down the Data Collection program Step 4 Restart the computer After logging in OrbixWeb Daemon should have automatically launched Do not start Data Collection at this time Step 5 a Turn off the instrument using the On Off button The following diagram shows the left front grill Reset button Greer Yellow Status lights Red OWO button b Wait 30 seconds c Turn on the instrument Step 6 When the green status light is steady wait 1 minute Step 7 Restart the 3700 Data Collection software Spatial Troubleshooting Module Page 30 of 30
12. h mod service module Refer to the Checking the CCD Possible error messages detected Window Module to determine if bubbles are still in the cuvette End Caps Blocked No Alignment Found Refer to the Checking for Bubbles Module if bubbles remain in system Spatial Troubleshooting Module Page 7 of 30 Applied Biosystems Possible Causes Recommended Actions Non Unique Alignment Cap Spacing Out of Range Replace the inline filter Array is not seated properly Verify that the array loading end header is seated correctly not under tension and the capillary tips are positioned in their injection wells Broken capillaries on the array Check the array for broken capillaries at the loading end the length of the array and the detection end of the array If breakage is observed replace the array Capillary Array has dried out and Run the Regenerate part of the Change Array wizard to clear the blockage it capillaries are blocked with may not work depending on the severity of the block polymer Replace with a new capillary array Leaking sheath flow syringe Inspect the sheath flow syringe for leakage at ports and within the barrel Replace sheath flow syringe if leakage is found Leaking inline filter Inspect the tubing around the inline filter for leakage indicated by dried polymer If leaking is observed replace the inline filter and run the Cuvette Flush module Refer to the Checking for Bubbles Module and the Checking for Le
13. inute i Opening cuvette to purge bubbles J Flush polymer through cuvette with polymer pump out valve 11 then out valve 12 Repeat flushing bubbles from cuvette Pressurize to 60 psi for 1 minute Flush polymer through cuvette with polymer pump out valve 11 then out valve 12 Third time flushing bubbles trom cuvette Pressurize to 60 psi for 1 minute Flush polymer through cuvette with polymer pump out valve 11 then out valve 12 Flush 1 5m polymer out valve 12 with sheath pump Flush 2ml polymer out valve 12 with polymer pump while sheath pump is running Filing load trough with water Ls Select Module Select Run Method Spatial Troubleshooting Module Page 21 of 30 Applied BS Biosystems ee Information When this window appears the module has completed if there is an error during the module please contact technical support Spatial Troubleshooting Module Page 22 of 30 Applied Biosystems ARRAY Loading End of Array The loading end header is the unit that holds the capillaries at the sample loading end of the capillary array and aligns them with the injection wells of the loading bar The diagram below shows how the loading end header is held in the loading block fo Loa ding end ae header Loading block D Clip Handle Loading bar Injection well S E apillaries foe A P Ae Spati
14. mproperly prepared spatial standard Entire run display has only one color blue or red Possible error messages Red Profile Error Blue Profile Error Blue and Red Profile Error End Caps Blocked No Alignment Found Sample loading issue Verify that the fittings on the ports above the sample transfer syringes are Bubbles in the sample finger tight transfer lines Verify that the sample transfer syringes are tightened to the pump Finger tight should be sufficient Water lines feeding the sample transfer pumps are Verify that the fluid line sinkers are at the bottom of the reservoir not underwater in the water reservoir Verify water levels in the water reservoir Replenish if necessary Run the Change tips wizard This will prime the syringes and flush water through the tubing going to the autoloader tips During the wizard monitor both sample transfer syringes for bubbles If bubbles are present in the syringe but not the tubing from the water reservoir change the syringe Clogged autoloader tips Replace the autoloader tips After installation verify that the tips are tightened until finger tight A non functioning sample Refer to the Verifying Syringe Pump Module transfer pump Random capillaries do not have Bubbles in the cuvette Verify that there is enough polymer in the polymer bottle for the run signal present prevented fluorescence from some capillaries from being Run the CuvetteFlus
15. number 4325775 The spatial buffer can be made by combining 100uL of 3700 10X buffer and 900uL of fresh HiDi formamide Ensure that the correct run module is being used for the polymer and run type SpatSQ2_ POP5DefaultModule for sequencing applications using POP5 polymer SpatSQ2_ POP6DefaultModule for sequencing applications using POP6 polymer SpatGS2 POP6DefaultModule for fragment analysis applications using POP6 polymer SpatSNP2 POP5DefaultModule for the SNPShot applications using POP5 polymer Monitoring a spatial run During a spatial run it is possible to monitor the run through the array view page This page provides a quick overview of a run and is useful in identifying hardware related issues that can affect the spatial If a spatial run has failed the spatial information can only be viewed through the entire run display window in Data Collection Software v2 0 Spatial Troubleshooting Module Page 1 of 30 Applied Biosystems Spatial Passes but quality is questionable Another spatial will have to be performed after performing the recommended actions Possible Causes Recommended Actions Left to Right Variation has a ratio Bubble in the cuvette Run the Cuvette Flush module Refer to the Checking for Bubbles Module greater than 1 3 and the Checking for Leaks Module if the bubbles in the cuvette persist Cuvette temperature needs Optimize cuvette temperature in 3 C increments The temperature can optimization range from 35 t
16. o 50 C Refer to page 4 16 of the ABI Prism 3700 DNA Analyzer User Guide for instructions on optimizing the temperature Pea bie ge ol pph psala willl Nn a 2 fe Capillary array alignment Verify that the array loading end header is seated correctly not under problem tension and the capillary tips are positioned in their injection wells Verify that the thumbscrews on the detection end base are not too tight finger tight is sufficient and are screwed on with equal tightness click to enlarge Capillary array damage Check the loading end of the array for damage and if necessary remove the array and check the detection end for broken capillaries If observed install a new array Set of four pattern Improperly preparing the spatial Prepare the 200uL of each color standard in a single tube vortex or pipette a ne standard mix and then pipette 100uL of standard into 2 tubes for running This I ensures that each tube is identical Spin down samples before placing on the instrument F AHSAN AAA II A a Sample loading issue Bubbles in the sample wells Spin down samples before placing on the instrument Inspect the tubes for bubbles in the bottom of the well click to enlarge Or Bubbles in the sample Verify that the fittings on the ports above the sample transfer syringes are transfer lines finger tight Spatial Troubleshooting Module Page 2 of 30 Applied Biosys
17. tems Pattern of highs and lows in this case eight high signals and Verify that the sample transfer syringes are tightened to the pump Finger eight low signals E tight should be sufficient Verify water levels in the water reservoir Replenish if necessary Verify that the fluid line sinkers are at the bottom of the reservoir Run the Change tips wizard This will prime the syringes and flush water through the tubing going to the loading tips During the wizard monitor both sample transfer syringes for bubbles If bubbles are present in the syringe but not the tubing from the water reservoir change the syringe click to enlarge Clogged autoloader tips Replace the autoloader tips Refer to the change tips wizard for instructions A non functioning sample Refer to the Verifying Syringe Pumps Module for more details transfer pump Broad peaks _ No Sheath Flow Check for leakage around the sheath flow syringe fittings Clean the area with a lint free tissue moistened with DI water and tighten the fittings until finger tight Refer to the Checking for Leaks Module Improperly mixed red standard Prepare the 200uL of each color standard in a single tube vortex or pipette mix and then pipette 100uL of standard into 2 tubes for running This ensures that each tube is identical Spin down samples before placing on the instrument Spatial Troubleshooting Module Page 3 of 30 Applied Biosystems Bub
18. un as this may cause unrecoverable screen update problems F700 Data Collection Software Version 1 1 Edi iew instrument Help Dala Acquistion d H lel Pinte Setup Run Setup Run Status Run Log Simus Aray Wiew Capilary view intensity we Time mira 0 i a 100 m ana aaa 264 Capillary Nurnber 74 Running EI Collecting Data Spatial Troubleshooting Module Page 11 of 30 Applied Biosystems Entire Run Display To view the Entire Run Display select the Data Acquisition menu gt select Display Entire Run E 4 1 Data Collechon Sollware Versin 2 0 Fie Echt View instrument Helo QRS Rei Override Spectral Calibration Plate Setup Run Setup Pun Status Set Color Status array views Capillary View Force Run Statue to Complete p Instrument Condition Display Reduced Data for Run instrument ID cemod 700 Display EPT Data for Run Number of Runs 0 Displey Spetel calibration C None Display Spectral Calibration The RunDisplayData folder should be open Highlight your run of interest and select OK E Choose File Look in RunDisplayData 3700 063 Run 3700 063 2002 08 05 149 gel Sf 00 063 Run_s 00 063_2002 06 05_20 ge Sf 00 063 Run_s700 063_2002 06 05_ 21 gel SF OO O63_ Run_s700 063 2002 08 05 22 gel Sf 00 063 Run_s 00 063_2002 06 06_23 9e Sf 00 063 Run_s700 063_2002 06 06_24 gel Sf 00 063
19. ush mad 3 Pipet estso4 mod E PipetT est96 mod a FlenumPurge mod Files of type All Files bi Cancel Applied Biosystems The service module utility should automatically search the Service Modules Folder if not the folder can be found in the D AppliedBio Support Files Data Collection Support Files Service Modules Double click on the module in this example CuvetteFlush mod Spatial Troubleshooting Module Page 20 of 30 Applied Biosystems After selecting run method the steps of the service module will be listed El Run Service Modules INSTRUM LAMAMA J eRe ons You must choose 4 file to run You selected module file CuvetteFlush mod Begin Cuyvette Flush Module Assumes cuvette and lines are filled with polymer The module selected should be listed Initializing sensors Flushing tubing to cuvette with POP Filling load trough with water Flushing tubing to cuvette with POP fe Run Service Modubes x Flush twice with polymer pump while the sheath pump is flushing J Waiting tor sheath pump J ee Flush polymer through cuvette out valve 11 You selected module fle Cuvetter kiah mod Waiting for sheath pump Flush 2 5 ml out valve 11 with sheath pump 1 Flush 4ml polymer out valve 11 with polymer pump while sheath pump is running Finished Sm Aaiting tor sheath pump q Allow pressure to return to zerg Flushing bubbles trom cuvette Pressurize to 60 psi for 1 m
20. uvette Minimal leakage Syringe may still be used but should be cleaned regulary and monitored If problems persists change syringe Severe leakage Replace syringe Dried polymer appears as a white residue inside of the barrel If present the dried polymer acts as an abrasive against the plunger and can damage the seal to the glass barrel If the seal is damaged bubbles can be introduced at this stage Replace the syringe using the Change syringe wizard Spatial Troubleshooting Module Page 26 of 30 Applied Biosystems Sample Transfer Syringe Port from water reservoir to syringe Port for syringe to autoloader tips Spatial Troubleshooting Module Page 27 of 30 Applied Biosystems Running the wizard Step 1 Select Instrument Step 2 Select Wizards Step 3 Select the appropriate wizard Instrument RUT Ctritk SKIH cte stap trit Wizards Change Array Utilities FO change Syringe Manual Control Change Polymer change Tips Longterm Autoloader Tips Autoloader tips Tips should be wiped in a downward direction to prevent clogging during cleaning Spatial Troubleshooting Module Page 28 of 30 Applied BS Biosystems Fluid Line Sinkers Fluid line sinkers Water Inline Filter Dried polymer will appear in these areas Inline filter Spatial Troubleshooting Module Page 29 of 30 Applied Biosystems Restarting the 3700 DNA Analyzer IMPORTANT To prevent
21. vette Run the Cuvette Flush module Refer to the Checking for Bubbles Module are causing bright events and the Checking for Leaks Module if the bubbles in the cuvette persist Spatial Troubleshooting Module Page 4 of 30 Applied Biosystems The next set of observations is for spatials that generate the following message E W aming IA Motenough good capillaries to schedule runs The message can be accompanied by the following errors Red Profile Error Blue Profile Error Blue and Red Profile Error End Caps Blocked No Alignment Found Non Unique Alignment Cap Spacing Out of Range Too Many Caps No Caps Found In these cases the spatial fails and does not display a profile in Data Collection V2 0 software It will be necessary to use the Entire Run Display feature to assist with troubleshooting Another spatial will have to be performed after performing the recommended actions Spatial Troubleshooting Module Page 5 of 30 Applied Biosystems Possible Causes Recommended Actions Entire run display is blank Restart the system Possible error messages Optics Check the CCD window for raman lines Refer to the Checking CCD No Caps Found Window module If raman lines are not present contact technical support Red Profile Error Blue Profile Error Blue and Red Profile Error Sample loading issue The four spatial tubes are Place the four tubes into wells 1 4 of the left 8 bar and spin down samples incorrectly position
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