User manual - Techne Calibration
Contents
1. C C ABILITY C DRIFT C 0to100 0 025 0 010 0 020 20 to 130 0 050 0 010 0 020 TIME CONSTANT 0 200 0 05 Seconds 63 of instantaneous ambient to 37 C shift Ambient temperature between 18 C and 28 C Traceable to NIST At or above 100 C for less than 50 hours year See CHK CAL operation on page 4 20 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS SPECIFICATIONS A MODEL 4500 WITH AN SP64X SmartProbe SP640 4 85 SP642 6 5 SP646 12 THERMISTOR 30k Ohms 25 C PROBE Wide range high accuracy general purpose stainless steel probe MEASUREMENT RANGE 40 00 C to 150 00 C 40 00 F to 299 00 F RESOLUTION 0 01 0 001 CALIBRATION PERIOD 12 months RANGE ACCURACY REPEAT ONE YEAR C C ABILITY C DRIFT C Oto 100 0 025 0 010 0 020 25 to 130 0 050 0 010 0 020 30to 150 0 100 0 020 0 030 40 to 150 0 150 0 020 0 030 TIME CONSTANT 3 0 5 Seconds 63 of instantaneous ambient to 37 C shift Ambient temperature between 18 C and 28 C Traceable to NIST At or above 100 C for less than 50 hours year See CHK CAL operation on page 4 21 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS SPECIFICATIONS A MODEL 4500 WITH AN SP212 SmartProbe THERMISTOR 10k Ohms 25 C PROBE 20 uL THERMAL CYCLER PROBE MEASUREMENT RANGE 20 00 C to 105 00 C 4 002. Press the F2 key F2 is now the DELTA T function Press F2 two more times to get back to normal operation Now F1 is STORE and F2 is DELTA T TAKING THE DELTA T READING Put your probe into beaker A Allow the reading to stabilize Press F1 the STORE function The STORE icon and the temperature numbers are flashing on and off This lets you know that you are about to put a value into memory erasing any previous value which had been stored When F1 is pressed a second time the value on the display is put into memory and the thermom 9 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS OPERATING INSTRUCTIONS 5 KEY PAD OPERATION Put the temperature probe into beaker B Press F2 DELTA T The DELTA T icon will appear and the numerical reading will show the difference between the stored value beaker A and the temperature of the present reading beaker B This is a real time delta measure ment As the temperature of beaker B changes you will see the difference between beaker B and the earlier stored value of beaker A Press F2 again The stored value is displayed beaker A Press F2 again and the thermom eter returns to normal operation Press F2 again and you will see the delta measurement again and so on 6 AUTO SHUTOFF The thermometer is factory set to automatically turn itself off 10 minutes after being turned on or 10 minutes after the last function key entry You may turn the
3. RS 232 INTERFACE The 4500 will display H232 on the LCD when properly connected to an active RS 232 port In a terminal program you will see a prompt gt _ indicating that the 4500 and PC are communi cating properly You may need to hit enter a few times to synchronize the communication link To see a list of the available commands type H on the keyboard followed by ENTER carriage return Follow the prompts given by the 4500 on the screen The T command will continuously send temperature readings about every 1 2 second to the P C in the format of SXXX XXX always in degrees Celsius S sign a minus sign is shown if a negative number is being displayed and no sign is shown if a positive number is being displayed XXX XXX up to three significant whole digits with always three significant decimal places i e 102 248 or 12 315 The 4500 will continue to send the temperature to the P C until the thermometer is turned off 12 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS BATTERY REPLACEMENT When replacing the battery place the unit onto it s face and remove the battery door at the bottom of the case Remove the battery by prying it out of the mounting terminals by using your thumb or forefinger Replace with a 9 Volt alkaline battery Duracell MN1604 or equiva lent The old battery may be pried out from the mounting terminals by using your thumb or fore finger When putting in the new batter
4. C 299 98 F Flashing battery Battery needs symbol replacement Some versions of 4500 Series SmartProbes will have different temperature ranges See the specifications in the back of this manual or those packed separately with the unit To ensure that erroneous results are not displayed the numerical segments are disabled all turned on or all off one hour of running time after the battery symbol starts flashing 4 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS OPERATING INSTRUCTIONS 4 FUNCTIONS TE i IT PBS ee h 4 LCD Readout HOLD Freezes the display at the present temperature when the HOLD function is acti vated MIN MAX Displays the lowest MIN and highest MAX temperature from the time of turning on the meter STORE Records a temperature when the STORE function is activated The STORE d value can be retrieved and displayed in the DELTA T function The value is kept in memory until it is replaced or the unit is turned off DELTA T Calculates and displays the present temperature minus the STOREd temperature C F Selects either degrees Celcius or Fahren heit as the units to be displayed CHK CAL CHECK CAL allows the user to display three significant decimal places on the LCD screen i e 001 degree resolution Tens and hundred units are not displayed in 001degree LCD readout mode The CHK CAL icon stays on while in this mode 5 ALPHA TECHNICS
5. F to 221 00 F RESOLUTION 0 01 0 001 CALIBRATION PERIOD Probe 12 months lt 50 hours at 100 C yr RANGE ACCURACY REPEAT ONE YEAR C C ABILITY C DRIFT C 4to99 0 100 0 010 0 100 Offset at cal points In circulating water bath with the following offsets 4 C 0 500 C 37 C 0 320 C 65 C 0 200 C 95 C 0 050 C TIME CONSTANT 1 0 5 Seconds 63 of instantaneous ambient to 95 C shift Ambient temperature between 18 C and 28 C Traceable to NIST At or above 100 C for less than 50 hours year See CHK CAL operation on page 4 22 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS SPECIFICATIONS A MODEL 4500 WITH AN SP230 SmartProbe THERMISTOR 10k Ohms 25 C PROBE 200 uL THERMAL CYCLER PROBE MEASUREMENT RANGE 20 00 C to 105 00 C 4 00 F to 221 00 F RESOLUTION 0 01 0 001 CALIBRATION PERIOD Probe 12 months lt 50 hours at 100 C yr RANGE ACCURACY REPEAT ONE YEAR C C ABILITY C DRIFT C 4to99 0 100 0 010 0 100 TIME CONSTANT 1 0 5 Seconds 63 of instantaneous ambient to 95 C shift Ambient temperature between 18 C and 28 C Traceable to NIST At or above 100 C for less than 50 hours year See CHK CAL operation on page 4 23 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS SPECIFICATIONS A MODEL 4500 WITH AN SP252 Smart
6. MIN MAX F C selection STORE for recording readings Delta T for measuring the difference between a stored value and the current reading Normal High resolution Big Display Large 6 LCD numbers allows for viewing All functions such as HOLD STORE Delta T F C etc are easily readable and logically arranged Low Noise Circuitry Patented circuitry allows for very clean low noise signals Even background electronic noise is substantially reduced Self heating lead resis tance thermistor anomalies and other factors are taken into account and corrected in the calcula tions Battery Door The 9 Volt Alkaline battery is easily accessable 2 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS RS232 d c input Connector connector 12V 20mA Probe input Figure 1 Rear Panel connections 3 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS OPERATING INSTRUCTIONS 1 Determine that a Alpha Technics SmartProbe is connected to the unit 2 Press the green colored rubber key marked ON OFF to turn the unit ON Pressing this key again will turn the unit OFF 3 READOUT Display Explanation C F symbols Reading is displayed in degrees C or F 40 00 to 150 00 40 00 to 299 98 Actual temperature in degrees Celsius Fahrenheit LO Temperature is below 40 00 C 40 00 F or a probe is not inserted HI Temperature is above 150 00
7. PRECISION TEMPERATURE 4500 SERIES THERMOMETERS OPERATING INSTRUCTIONS 5 KEY PAD OPERATION MODE F1 F2 Bat J Key Pad All functions can be accessed through the MODE and FUNCTION F1 amp F2 keys The user can assign any of the aforementioned functions to either the F1 or F2 keys by scrolling through the functions using the MODE key then pressing either F1 or F2 LCD returns to normal 0 FANS HOLD Lj 001 resolution we ZN Shows Max s ARN eo ra ooo 7 The LCD Ny temperature CHK CAL is frozen MIN MAX Le A __7 01 resolution Shows eee of N temp MODE Flashing pis A resent kmp fe N Present temp minus the vA STORE S TOREJ y Ei emp lt Dez A Store the presen lemperature Ra A ees Shows STOREd temp Key pad functional flow chart 6 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS OPERATING INSTRUCTIONS 5 KEY PAD OPERATION When the MODE key is pressed the active function icon flashes and the other function icons turn on solid Pressing MODE again scrolls the display to the next function When the desired function icon is flashing pressing F1 or F2 assigns that function to the selected key and enters the thermometer into that selected function mode If a function has not been selected the MODE operation will automatically end after four flashes of the display about 4 secs or if MOD
8. unit off at any time by pressing the ON OFF switch Using a terminal program on a PC with an RS 232 interface this auto shut off can be disabled or set to any time between 3 and 998 minutes See pages 11 and 12 RS 232 INTERFACE When using the RS 232 interface or the optional external power supply the auto shutoff feature is automatically disabled 10 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS RS 232 INTERFACE The RS 232 interface allows temperature data from the 4500 Series thermometers to be sent to a standard Personal Computer An optional software package known as ThermalView allows for easy data capture and manipulation in a Windows environment Other data capture programs may be used When using non ThermalView data capture programs the following information may be necessary The 4500 Series thermometers enter the HOST mode when the RTS line becomes active true When the RTS line becomes FALSE the 4500 returns to the normal mode of operation Baud Rate 9600 Data Length 8 Bits Parity None Stop Bits 1 Hardware None The 4500 has a standard DB9 female connector with the following pins me z DIRECTION SIGNAL NAME No Connection to Host RX Receive Data From Host TX Transmit Data No Connection GND No Connection From Host RTS Request to Send No Connection No Connection OONDUBRWNH 11 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS
9. 4500 SERIES THERMOMETERS INTRODUCTION vvcssssssssssssssessssssessessseenssee 1 OPERATING INSTRUCTIONS vsssssssseeeseeee 4 RS 232 INTERFACE 11 BATTERY REPLACEMENT sssssssesssssseesssseee 13 CARE AND CLEANING sssssssssesssssseesesseee 14 RECALIBRATION sssini 15 REPAIR isasagad LIMITED WARRANTY ccsssssssssessessseessssseee 17 SPECIFICATIONS 4500 cssssssssevsesssssssseesenss 18 SMARTPROBE SYSTEM SPECIFICATIONS SP110 MICRO PROBE sessssssssssssssssessseesssee 19 SP130 MICRO PROBE sissien 20 SP64X GENERAL PURPOSE PROBE 21 SP212 THERMAL CYCLER PROBE 22 SP230 THERMAL CYCLER PROBE 23 SP252 THERMAL CYCLER PROBE 24 TES veces turf iedce eect cence 25 ACCESSORIES st xasuoracsamianieensionitenc 26 GLOSSARY ssisscici cusasssisagansctennemnetuaes 27 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS INTRODUCTION FUNCTIONS amp FEATURES o Ultra High Instrument Accuracy up to 0 005 C o High Resolution up to 0 001 C o Wide Range 40 C to 150 C o Accepts a variety of thermistor probes o Hold Min Max Delta T Store F C functions o Selectable Auto Shut Off Period Manual Shut Off o Over Under Range Indication o Low Battery Indication o RS 232 Output o User Friendly Key Pad o User Assigned Special Functions o NIST Traceable The 4500 series thermometers are multipurpose ultrahigh accuracy high resolution hand held digital thermometers T
10. E is scrolled past CHK CAL 7 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS OPERATING INSTRUCTIONS 5 KEY PAD OPERATION Example 1 You want F1 to be the F C function and F2 to be the HOLD function Turn on the unit After the unit has initialized press the MODE key down 5 times C or F will be flashing Press F1 F1 is now the degrees C F function key The thermometer has also changed from C to F or from F to C Now assign HOLD to the F2 key Press MODE once HOLD is flashing Press F2 F2 is now the HOLD function The thermometer has also been put into the HOLD mode Press F2 again to go back to normal mode Example 2 You want to take Delta T measure ments The difference between a value you have stored using the STORE function and the meter s present reading In this example let us assume that you are taking the temperature difference between two beakers of water beaker A and beaker B 8 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS OPERATING INSTRUCTIONS 5 KEY PAD OPERATION First you need to assign the STORE function to the F1 key and the DELTA T function to the F2 key You can assign them differently if you wish Press the mode key 3 times The STORE icon is flashing Press the F1 key F1 is now the STORE func tion Press F1 again to return to normal operation Press the MODE key 4 times The DELTA T icon is flashing
11. Probe THERMISTOR 10k Ohms 25 C PROBE 500 uL THERMAL CYCLER PROBE MEASUREMENT RANGE 20 00 C to 105 00 C 4 00 F to 221 00 F RESOLUTION 0 01 0 001 CALIBRATION PERIOD Probe 12 months lt 50 hours at 100 C yr RANGE ACCURACY REPEAT ONE YEAR C 4 C ABILITY C DRIFT C 4to99 0 100 0 010 0 100 TIME CONSTANT 1 0 5 Seconds 63 of instantaneous ambient to 95 C shift Ambient temperature between 18 C and 28 C Traceable to NIST At or above 100 C for less than 50 hours year See CHK CAL operation on page 4 24 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS NOTES 25 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS ACCESSORIES 145600 ThermalView Data Acquisition Software 140621 RS 232 Cable 10 cable to connect the 4500 to a PC 139221 Carrying Case Space for the 4500 and a probe 138724 Deluxe Carrying Case Space for the 4500 several probes power supply ThermalView software Cal plugs etc 26 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS GLOSSARY ACCURACY The degree of absolute conformity to a standard NIST National Institute of Standards and Technology PRECISION The accuracy with which a number can be represented sharply defined conforming to a pattern tolerance REPEATABILITY The ability of a probe or instrument to give the same output or reading und
12. er repeated identical conditions within an 8 hour period RESOLUTION The least significant digits shown on a digital display RS 232 A standard computer interface protocol SENSOR SELF HEATING The internal heat gener ated in the sensor by passing a current through the sensor to measure its resistance TEMPERATURE STANDARD An instrument who s calibration is directly traceable to the NIST STABILITY The ability of a device to maintain a constant output with the application of a constant input SYSTEM ACCURACY The total accuracy of the probe plus the accuracy of the thermometer SYSTEM CALIBRATION Calibration of a thermom eter and probe combination THERMISTOR A temperature sensing element composed of semiconductor material whose resistance varies greatly in a known manner with the change of temperature The 4500 uses negative temperature coefficient thermistors as temperature increases resistance decreases PN 139908 Rev A 27 ALPHA TECHNICS PRECISION TEMPERATURE pn139908 RevA
13. he 4500 series thermometers accept a wide range of plug in probes equipped with Al pha Technics proprietary SmartProbe system Each SmartProbe has it s own calibration data stored in the SmartProbe adapter Ultrahigh system accuracy is achieved because the thermometer and probe function as if they were calibrated together as a system Along with HOLD degrees F C selection MIN MAX STORE DELTA T Normal High resolution and low battery indication features the RS 232 port can also download data from the thermometer to a standard PC Data can be manipulated and sent to other programs using the optional software package ThermalView Thoughtful design has resulted in a user friendly enclosure and key pad format All functions can be easily accessed through the MODE and FUNCTION keys Functions are user assigned to desired keys 1 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS INTRODUCTION Large Probe Selection The Model 4500 accepts Alpha Technics high accuracy thermistor probes and a variety of other thermistor probes with SmartProbe termination Computer Interface An RS 232 computer interface allows the user to download Information from the Model 4500 to their own PC to create reports and graphs PC software called ThermalView is available Friendly Key Pad A user friendly KEY PAD allows the operator to program the most frequently used functions into the unit The functions include HOLD
14. ied by Alpha AN Technics then the protection pro vided by the instrument may be impaired 14 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS RECALIBRATION The Model 4500 Thermometer should be recalibrated at least once every two years A SmartProbe should be recalibrated at least once a year to maintain its accuracy Some SmartProbes may require a 6 month recalibration cycle Check the Report of Calibration which came with the thermometer or the recalibration label on the unit to deter mine the next recalibration due date SmartProbes will display the recalibration date on the LCD screen of a Model 4500 when powered up Recalibration should be per formed at Alpha Technics When the Thermom eter requires recalibration forward it postage paid to Alpha Technics Att Service Repair 1560 Orangethorpe Way Anaheim CA 92801 Phone 714 578 9205 Fax 714 773 9327 Contact Alpha Technics for current prices and include with the unit a purchase order a ship to bill to address a phone number and a contact name Ship the unit in its original carrying case or box enclosed in a suitable shipping con tainer 15 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS REPAIR If the Thermometer fails forward it postage paid to Alpha Technics Include a description of the difficulty place the Thermometer in its carrying case or box and pack the unit securely in a suitable sh
15. ipping container Alpha Technics shall assume NO responsibility for damage in transit IN WARRANTY Instruments covered by the limited warranty will be promptly repaired or replaced at Alpha Technics option and returned at no charge SEE LIMITED WARRANTY PAGE 17 FOR ITEMS COVERED AND COMPLETE WAR RANTY TERMS Other manufacturers probes are not included under Alpha Technics warranty unless otherwise stated OUT OF WARRANTY The Thermometer will be repaired and returned for a fixed fee Repairs needed because of abuse or accidental damage will be quoted Contact Alpha Technics for current prices Include a purchase order with the unit 16 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS WARRANTY ALPHA TECHNICS THERMOMETER 1 YEAR LIMITED WARRANTY The thermometer manufacturer Alpha Technics warrants your thermometer to be free from defects in material and workmanship under normal use and service The warranty period for the Thermometer is 1 YEAR from the date of purchase and DOES NOT apply to batteries or when the instrument has been misused altered or damaged by accident or abnor mal conditions of operation For warranty service send the instrument with a description of the difficulty postage prepaid to Alpha Technics Alpha Technics assumes no risk for damage in transit Alpha Technics will at its option repair or replace the defective instrument free of charge However if we dete
16. rmine that the failure was caused by misuse alteration accident or abnormal condition of operation you will be billed for the repair The repaired instrument will be returned postage prepaid ALPHA TECHNICS MAKES NO WARRANTY IN CLUDING IMPLIED WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE OTHER THAN THE WARRANTY STATED ABOVE ALL WARRANTIES ARE LIMITED TO A PERIOD OF 1 YEAR FROM THE DATE OF PUR CHASE ALPHA TECHNICS SHALL NOT BE LIABLE FOR ANY SPECIAL INCIDENTAL OR CONSEQUEN TIAL DAMAGES WHETHER IN CONTRACT TORT OR OTHERWISE Note U S A only Some states do not allow limitations of implied warranties or exclusion of incidental or consequen tial damages so the above limitations or exclusions may not apply to you This warranty gives you specific legal rights and you may have other rights which vary from state to state 17 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS SPECIFICATIONS GENERAL INSTRUMENT SPECIFICATIONS ENVIRONMENTAL CONDITIONS Operating Temperature 0 40 C 32 to 104 F Humidity 0 85 Storage Temperature 0 60 C 32 to 140 F Humidity 0 70 READING RATE 2 sec one reading every 524msec DISPLAY 4 1 2 digit LCD POWER 9 VDC battery alkaline Type PP3 or equivalent Auto shut off time 10 minutes Auto shut off can be disabled or shut off time changed via the RS 232 port Optional 110VAC adapter is available External uni
17. versal PSU is available as an option 12V 20Ma 79 BATTERY LIFE 20 hours typical 15 hours using RS 232 battery symbol flashes when less than one hour running time remains All LCD segments turn on or off after the one hour of running time DIMENSIONS 210H x 108W x 44D mm 8 25 H x 4 25 W max x 1 75 D max WEIGHT 405g 140z 18 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS SPECIFICATIONS A MODEL 4500 WITH AN SP110 SmartProbe THERMISTOR 10k Ohms 25 C PROBE Mid range high accuracy micro probe MEASUREMENT RANGE 20 00 C to 55 00 C 4 00 F to 131 00 F RESOLUTION 0 01 0 001 CALIBRATION PERIOD 12 months RANGE ACCURACY REPEAT ONE YEAR C C ABILITY C DRIFT C 0to50 0 025 0 010 0 020 20to55 0 060 0 010 0 030 TIME CONSTANT 0 200 0 05 Seconds 63 of instantaneous ambient to 37 C shift Ambient temperature between 18 C and 28 C Traceable to NIST Probe not exposed to temperatures above 50 C See CHK CAL operation on page 4 19 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS SPECIFICATIONS A MODEL 4500 WITH AN SP130 SmartProbe THERMISTOR 22k Ohms 25 C PROBE Extended range high accuracy micro probe MEASUREMENT RANGE 20 00 C to 130 00 C 4 00 F to 266 00 F RESOLUTION 0 01 0 001 CALIBRATION PERIOD 12 months RANGE ACCURACY REPEAT ONE YEAR
18. y be sure that the terminals line up and snap firmly into the terminal on the circuit board The unit will not be damaged if you accidentally try to put the battery in backwards Replace the battery door 13 ALPHA TECHNICS PRECISION TEMPERATURE 4500 SERIES THERMOMETERS CARE AND CLEANING Extensive testing has proven this case to be rugged and reliable Itis splash resistant but not water proof The case may not be sub merged Liquid will flow in and damage or ruin the thermometer DO NOT AUTOCLAVE THE UNIT If it is necessary to decontaminate the thermometer a weak chlorine solution may be gently wiped on to the case by a well rung out soft cloth Do not let any liquid go into the jacks Your readings will be affected The clear plastic LCD lens can be easily scratched and or fogged by inappropriate cleaning products Use only plastic approved lens cleaners Alcohol acetone lacquer thinners and other harsh chemicals will fog the lens and damage the case and rubber key pad Keep the 8 pin DIN receptacle on the Model 4500 and the pins on the SmartProbe clean Periodically wipe off and clean the probe plug contacts Be sure to ground yourself before touching the pins on the SmartProbe adapter There are active electronic components inside Dirty contacts will cause reading errors Warning Never use Alcohol or AN thinners as these will damage the instrument Warning If Model 4500 is used in a manner not specif
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