Agilent 7890A GC Operating Guide
Contents
1. Figure4 3 layer sandwich injection When loading vials for sandwich injections layer 1 L1 can be placed in any turret position 1 14 if using a standalone turret or in any tray position 1 150 if using a transfer turret with sample tray The vials for layer 2 L2 and layer 3 L3 must be placed in turret positions L2 and L3 Figure 5 respectively Standalone turret Transfer turret Figure5 Turret vial locations for sandwich injections 53 5 Operating the 7693A Automatic Sampler To Park the Injector To park an injector safely set it aside so it is not used simply place it on an installed parking post G4513 20562 Ne p Bo 54 Operating Guide Operating the 7693A Automatic Sampler 5 To Install a Syringe on the 7693A ALS Operating Guide To install a syringe Figure 6 Plunger carrier Slide Plunger screw Syringe carrier 1 Plunger Flange guide Flange Syringe latch Barrel Needle Needle support foot Figure 6 Installing a syringe Unplug the injector cable and if desired mount the injector on a parking post or lay the injector tower on a work bench Open the injector door Slide the syringe carriage to the top position Open the syringe latch by swinging it in a counterclockwise direction Lift the plunger carrier to the top position Carefully pass the syringe needle through the guide hole in the ne2. 7 Rotate the turret clockwise until it stops then verify the installation by manually sliding the syringe carriage down until the needle enters the inlet Operating Guide 79 6 Operating the 7683 Automatic Sampler To Install a 200 pm Column With the COC Inlet Agilent recommends using a 530 pm retention gap for injections directly onto 200 ym columns 1 Prepare the inlet See To Prepare for Inlet Maintenance Be careful The oven and or inlet may be hot enough to cause burns If either is hot wear heat resistant gloves to protect your hands 2 Install a short 530 um retention gap uncoated fused silica tubing into the inlet See To Install a Capillary Column with the COC Inlet 3 Attach the retention gap to the 200 um column using an Agilent Ultimate Union Kit part numbers G3182 61580 and G3182 61581 To Manually Inject Onto a 200 pm Column With the COC Inlet 80 CAUTION 1 If needed prepare the column See To Install a 200 um Column With the COC Inlet These steps should be done smoothly with minimal delay 2 Immerse the syringe needle in the sample pump the syringe plunger to expel air from the barrel and needle 3 Draw the sample into the syringe 4 Remove the needle from the sample and draw about 1 uL of air into the syringe 5 Wipe the needle dry if it is wet 6 Guide the needle straight into the septum nut pierce the septum and insert the needle fully into the inl
3. 63 5 Operating the 7693A Automatic Sampler 64 Operating Guide Agilent 7890A Gas Chromatograph Operating Guide 6 Operating the 7683 Automatic Sampler 7683 ALS Turret Types 66 To Change the 7683 ALS Turret 67 To Move the 7683 ALS to the Other GC Inlet 69 To Park the Injector 71 To Install a Syringe on the 7683 ALS 72 To Remove the Syringe on the 7683 ALS 74 To Configure Solvent Waste Vials 75 To Load Vials in the 7683 ALS Turret 77 To Load Vials in the 7683 ALS Sampler Tray 77 7683 ALS Prerun Checklist 78 To Adapt the 7683 ALS for the COC Inlet 79 To Install a 200 um Column With the COC Inlet 80 To Manually Inject Onto a 200 um Column With the COC Inlet 80 This section describes the basic operation of the 7683 automatic liquid sampler ALS The procedures here expand on the information provided in the Agilent 7683B Automatic Liquid Sampler Installation Operation and Maintenance manual ois Agilent Technologies 65 6 Operating the 7683 Automatic Sampler 7683 ALS Turret Types 66 The injector comes with two turrets a standard 1 sample transfer turret for use with or without a tray and an 8 sample turret to be used without a tray Hi density turret Transfer turret 1 sample Top views Labels are on sides The labeled positions are defined in Table 4 and Table 3 Table 3 The 8 sample vial turret Name Position Description 1 through 8 1 through 8 Sample vials WA 9 Waste bottle B 1
4. When performing cool on column injections the injector Slows the carriage speed so the overall injection time increases to 500 milliseconds Lowers the tip of the syringe needle an additional 19 mm into the column To adapt the injector and GC for cool on column use follow these steps 1 Operating Guide If necessary remove the current syringe from the injector See To Remove the Syringe on the 7693A ALS for more information If necessary remove the injector from the mounting post and unplug the injector cable If desired park it on a parking post Select the on column syringe needed for the column size See your Agilent catalog for consumables and supplies your GC operating documentation and the Agilent web site www agilent com chem for a list of parts Replace the needle support foot with the supplied on column foot See the Agilent 7693A Automatic Liquid Sampler Installation Operation and Maintenance manual for more information Install the on column syringe See To Install a Syringe on the 7693A ALS for more information Prepare the GC inlet See your GC operating documentation for instructions Check your needle to column size Verify that the insert matches the needle size If necessary replace the septum For a list of other consumables needed to perform these injections see your GC operating documentation Plug in the injector cable Install the injector on the mounting post
5. if you use only solvent A washes for the second sample the solvent B2 wash vial is skipped When running single samples only solvent wash vials A and B are used Syringe size Enter a syringe size at full plunger stroke between 1 and 100 uL 75 76 Operating the 7683 Automatic Sampler The G2913A injector always uses both WA waste bottles if a solvent A wash is used and both WB waste bottles if a solvent B wash is used Waste bottle mode G2613A This is only displayed when the G2613A injector has a 3 vial turret installed The turret waste bottle positions are controlled using the Mode Type key e Use both A and B alternates between the two waste bottles Use only A bottle uses waste bottle A only e Use only B bottle uses waste bottle B only Use B2 wash G2613A This is only displayed if you have the 3 vial turret installed in the G2613A injector You can enable this option to use two 4 mL vials of solvent B increasing the number of runs you can make before refilling solvent vials Press the On button to enable B2 wash e Use the same solvent in position B and in position B2 This option does not enable you to use a third solvent e When using solvent A and solvent B both waste bottles are required e Your maximum solvent capacity is 6 mL 2 mL each for solvent vials A B and B2 e Configure each injector separately Note that the number of solvent B washes for each injection does not change The
6. Operating Guide The GC Column and Oven Operating Guide Introduction 1 GC columns are located inside a temperature controlled oven Generally one end of the column is attached to the inlet while the other end is attached to the detector Columns vary in length diameter and internal coating Each column is designed for use with different compounds The purpose of the column and the oven is to separate the injected sample into individual compounds as it travels through the column To aid this process the GC oven can be programmed to speed the sample flow through the column 1 Introduction Capillary Flow Technology 16 Agilent capillary flow technology CFT devices are used for splitting heart cutting and reliable zero dead volume connections The features of the capillary flow technology make traditionally difficult connections simple reliable and leak free The optional CFT switches splitters and QuickSwap accessories are located on the inside of the oven wall These devices are primarily used when the analysis requires multiple sample paths between the inlet and detectors They allow the chromatographer to design very efficient sample paths using multiple columns or detectors In addition analysis time can be reduced with the benefit of backflushing Operating Guide Introduction 1 Detectors Detectors identify the presence of compounds as they exit the column As each compound enters the d
7. This is only displayed when the injector has a transfer turret installed The turret solvent bottle positions are selected using the Mode Type key 6 A and 4 B Washes Uses all 6 solvent A bottles if injector uses solvent A washes Uses all 4 solvent B bottles if injector uses solvent B washes The injector alternates between all bottles 2 A and 2 B Washes Uses solvent bottles A and A2 if injector uses solvent A washes and solvent bottles B and B2 if injector uses solvent B washes The injector alternates between all bottles 1 A and 1 B Washes Uses solvent bottle A if injector uses solvent A washes and solvent bottle B if injector uses solvent B washes The injector always uses both WA waste bottles if a solvent A wash is used and both WB waste bottles if a solvent B wash is used Syringe size Enter a syringe size between 0 5 and 500 uL The GC assumes the syringe volume represents full plunger stroke If the maximum volume marked on the syringe barrel is halfway up the barrel you will need to enter double that volume the label in this case is half of the full plunger stroke Operating Guide 59 5 Operating the 7693A Automatic Sampler 7693A ALS Prerun Checklist Use this checklist before running analyses to make sure that the sample vials and sampler are ready o u o o u u o u o m u u o u 0 O 60 Sample vials are at least half full Vial cap is centered with no wrinkles and the
8. is the active line in the display has a lt next to it Info will display the valid range of oven temperatures In other cases Info will display definitions or actions that need to be performed SETPOINT SETPOINT Operating Guide 41 4 Keypad Operation The General Data Entry Keys Mode Type Accesses a list of possible parameters associated with a component s nonnumeric settings For example if the GC is configured with a split splitless inlet and the Mode Type key is pressed the options listed will be split splitless pulsed split or pulsed splitless Clear Removes a misentered setpoint before pressing Enter It can also be used to return to the top line of a multiline display return to a previous display cancel a function during a sequence or method or cancel loading or storing sequences and methods Enter Accepts changes you enter or selects an alternate mode Scrolls up and down through the display one line at a time The lt in the display indicates the active line Ged Gee Numeric Keys Are used to enter settings for the method parameters Press Enter when you are finished to accept the changes On Yes Are used when you are setting up parameters 0ff No such as the warning beep method modification beep and key click or for turning on or off a device like a detector Front Back Are mostly used during configuration operations For example when config
9. or resumes a sequence or views the status of a sequence See the Advanced User Guide for details Sample Tray Displays whether the tray and or bar code Prog User Key 1 User Key 2 reader is enabled Allows you to program a series of keystrokes commonly used for specific operations See the Advanced User Guide Operating Guide Keypad Operation 4 Keypad Functionality When the GC Is Controlled by an Agilent Data System Operating Guide When an Agilent data system controls the GC the data system defines the setpoints and runs the samples If configured to lock the keypad the data system can prevent the changing of setpoints The Remote LED is lit when a data system is controlling the GC Lit LEDs on the status board show the current progress of a run When an Agilent data system controls the GC the keypad can be used To view run status by selecting Status To view the method settings by selecting the GC component key To display the last and next run times the run time remaining and the post run time remaining by repeatedly selecting Time To abort a run by selecting Stop To find which computer is controlling the GC by pressing Options gt Communication then scrolling The name of the computer controlling the GC is listed under the Enable DHCP setting along with the number of hosts connected to the GC 45 4 Keypad Operation About GC Status Status Board Alert tones 46 When
10. require user intervention Depending on the type of error the GC will beep once or not at all Blinking setpoint If a gas flow multiposition valve or the oven is shut down by the system Off or On Off will blink on the appropriate line of the components parameter listing Operating Guide Introduction 1 The keypad All of the parameters required to operate the Agilent 7890A GC can be entered through the GC s keypad Normally however most of these parameters are controlled using an attached data system such as the Agilent ChemStation When the Agilent ChemStation is controlling your Agilent 7890A GC it is possible for the ChemStation to disable editing of the GC s current method from the keypad The Run Key oven Cor GC Component ji J Det Keys a a eee SS Valve Col 2 ac Analog pa 4 d Servico wa General Data Entry Keys Supporting Keys Method Storage and Automation Keys Operating Guide 21 1 22 Introduction Operating Guide Agilent 7890A Gas Chromatograph Operating Guide 2 Operating Basics Overview 24 Instrument Control 25 Correcting Problems 26 To Start Up the GC 27 To Shut Down the GC for Less Thana Week 28 To Shut Down the GC for More Thana Week 29 This section describes the tasks that an operator performs when using the Agilent 7890A GC Phe Agilent Technologies 23 2 Operating Basics Overview 24 Operating the GC involves the
11. septum is flat Sample vial positions match the run parameters Each solvent bottle contains 4 5 mL of fresh solvent Waste bottles are empty Syringe door is closed Sufficient solvent and waste capacity is available for sample vials Syringe is new or clean Syringe design and size are correct Plunger is secure in plunger carrier loop Needle is aligned with septum retainer nut Syringe is rinsed with solvent GC inlet liner is clean and deactivated GC inlet liner or insert is correct type for injection technique GC inlet septum type is correct GC inlet septum has sufficient life remaining Correct septum nut is installed in GC inlet Operating Guide Operating the 7693A Automatic Sampler 5 To Move the 7693A ALS to the Other GC Inlet 1 When no runs are scheduled lift the injector tower off the mounting post 2 If a tray is installed disconnect the communications cable from the injector tower 3 Set the injector tower aside Place on a parking post or lay it flat on its back CAUTION Use a flathead screwdriver that snugly fits the slot in the top of the mounting post An undersized blade can damage the post top and prevent the injector from mounting properly 4 Remove the mounting post from the inlet cover Install it at the other location The post must be tightened completely 5 If a sample tray is installed route the front injector cable through the mounting bracket support structure If in
12. tS 01150234 Agilent 7890A Gas Chromatograph Operating Guide ee Agilent Technologies Notices Agilent Technologies Inc 2007 2010 No part of this manual may be reproduced in any form or by any means including electronic storage and retrieval or transla tion into a foreign language without prior agreement and written consent from Agi lent Technologies Inc as governed by United States and international copyright laws Manual Part Number 63430 90011 Edition Third edition June 2010 Second edition September 2008 First edition March 2007 Printed in USA or China Agilent Technologies Inc 2850 Centerville Road Wilmington DE 19808 1610 USA Agilent Technologies Inc 412 Ying Lun Road Waigoagiao Freed Trade Zone Shanghai 200131 P R China Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user ha
13. the GC is ready to begin a run the display screen shows STATUS Ready for Injection Alternatively when a component of the GC is not ready to begin a run the Not Ready LED is lit on the status board Press Status to see a message explaining why the GC is not ready N a Ragay Fun Oven Ramp Final Temp Remote n Post num L Clock Run Pre w Table Log Run Initial Temp Gas Service Saver Due A lit LED on the status board indicates The current progress of a run Pre Run Post Run and Run Items that may require attention Rate Not Ready Service Due and Run Log The GC is controlled by an Agilent data system Remote The GC is programmed for events to occur at specified times Clock Table The GC is in gas saver mode Gas Saver A series of warning beeps sounds before a shutdown occurs After a short time the component with the problem shuts down the GC emits one beep and a brief message is displayed For example a series of beeps sounds if the front inlet gas flow cannot reach setpoint The message Front inlet flow shutdown is briefly displayed The flow shuts down after 2 minutes Press Clear to stop the beep Operating Guide Keypad Operation 4 A continuous tone sounds if a hydrogen flow is shut down or a thermal shutdown occurs WARNING Before resuming GC operations investigate and resolve the cause of the hydrogen shutdown See Hydrogen Shutdown in the Troubles
14. the syringe into place keeping the needle in the hole of the needle support foot Close the syringe latch by swinging it clockwise Plunger screw me Hr 8 Plunger a Flange guide ay Flange Syringe carrier Nr T DEST Syringe latch open c ae Barrel Syringe clip AIN Needle Needle support foot _ 7 Loosen the plunger screw until the stop is reached 10 Move the plunger carrier loop down and tighten the plunger screw Move the plunger carrier loop up and down If the syringe plunger does not move along with the plunger carrier loop repeat steps 4 through 8 Be sure the plunger screw is tight Check that the needle is aligned with the needle guide in the needle support foot by moving the slide up and down The needle should slide smoothly in the needle guide Operating Guide Operating the 7683 Automatic Sampler 6 q ij je l i Syringe latch closed S oe mi mat Needle support jij CAUTION Do not operate the injector without a syringe in place The syringe latch may interfere with the motor if the latch is allowed to
15. three major steps involved with separating and identifying components of a mixture using a GC They are 1 Injecting a sample into the GC This takes place at the inlet 2 Separating the sample into individual components This takes place inside the column in the oven 3 Detecting what compounds were in the sample This is done in the detector During this process status messages from the Agilent 7890A GC are displayed and user changes to parameter settings can be made through the operating panel Inlet Oven Detector Operating panel Each part of this process is described in brief on the following pages of this document Refer to the Advanced User Guide for more details 10 Operating Guide Introduction The Front View of the Agilent 7890A GC Detector cover Detectors Inlets Valves not shown Display Keypad Power switch Oven Latch The Back View of the Agilent 7890A GC Operating Guide Oven exhaust vent Inlet and detector vent Gas supply connections Electronic cable connections Oven cooling inlet Power connection 1 1 Introduction The Inlets Inlets are where samples are injected into the GC The Agilent 7890A GC can have a maximum of two inlets identified as Front Inlet and Back Inlet A complete selection of inlets split splitless 0 100 psi and 0 150 psi multimode purged packed cool
16. 0 Solvent bottle B A 11 Solvent bottle A Table4 The 1 sample vial transfer turret Name Position Description SAMPLE 1 Sample vial transfer position WB 2 and 3 Waste bottle B WA 4and5 Waste bottle A B3 6 Solvent bottle B3 B2 7 Solvent bottle B2 B 8 Solvent bottle B A3 9 Solvent bottle A3 A2 10 Solvent bottle A2 A 11 Solvent bottle A Operating Guide Operating the 7683 Automatic Sampler 6 To Change the 7683 ALS Turret 1 If the GC does not have a tray proceed to step 2 Otherwise do the following If changing from the 1 sample transfer turret to the 8 sample turret press Sample Tray Off No If changing from the 8 sample turret to the 1 sample turret for tray use press Sample Tray On Yes 2 Open the injector door Unscrew and remove the knurled nut from the top of the turret 3 Rotate the turret so that the open section faces and the arrow on top of the turret points to the back of the injector Push the stripper arm toward the back then lift the front of the turret so that it clears the center shaft Remove the turret Knurled nut Motor hub Stripper arm 4 Push the stripper arm to the back of the tower Insert the turret with the open section facing and the arrow pointing to the back of the injector 5 Seat the turret on the hub rotating it gently until it drops partially into position then push it down The top of the threaded shaft will be almost level with the top of the t
17. Agilent provides several learning products that document how to install operate maintain and troubleshoot the Agilent 7890A GC Before operating your GC be sure to read the safety and regulatory information included on the Agilent GC and GC MS Hardware User Information amp Utilities DVD The most common safety hazards when working on the GC are e Burns caused by touching heated areas on or in the GC e Release of pressurized gas containing hazardous chemical compounds caused by opening inlets e Glass cuts or puncture wounds caused by sharp capillary column ends e Use of hydrogen as a GC carrier gas Online User Documentation Now your Agilent instrument documentation is in one place at your fingertips The Agilent GC and GC MS Hardware User Information amp Utilities DVD that ships with your instrument provides an extensive collection of online help videos and books for current Agilent gas chromatographs mass selective detectors and GC samplers Included are localized versions of the information you need most such as e Getting Familiar documentation e Safety and Regulatory guide e Site Preparation information e Installation information Operating Guide Introduction 1 e Operating guides e Maintenance information e Troubleshooting details Operating Guide 9 1 Introduction Chromatography Using a GC Chromatography is the separation of a mixture of compounds into individual components There are
18. Keypad Loading Storing and Running Methods from the Keypad 32 Toloadamethod 32 To store a method 32 To manually inject a sample with a syringe and starta run 32 To run a method to process a single ALS sample 32 To aborta method 33 Loading Storing and Running Sequences from the Keypad 34 To load a sequence 34 To store a sequence 34 Operating Guide To start running a sequence 34 To pause a running sequence 35 To resume a paused sequence 35 To stop a running sequence 35 To resume a stopped sequence 35 Aborting a sequence 35 To resume an aborted sequence 36 4 Keypad Operation The Run Keys 38 The Service Mode Key 38 The GC Component Keys 39 The Status Key 40 The Info Key 41 The General Data Entry Keys 42 The Supporting Keys 43 Method Storage and Automation Keys 44 Keypad Functionality When the GC Is Controlled by an Agilent Data System 45 About GC Status 46 Status Board 46 Alerttones 46 Error conditions 47 Blinking setpoint 47 About Logs 48 Maintenance log 48 5 Operating the 7693A Automatic Sampler Placing Vials in the 7693A ALS 50 Placing vials in the sample tray 51 Placing vials in the turret no sample tray 52 Placing vials for sandwich injections 52 To Park the Injector 54 To Install a Syringe on the 7693A ALS 55 To Remove the Syringe on the 7693A ALS 58 To Configure Solvent Waste Vials 59 7693A ALS Prerun Checklist 60 Operating Guide To Move the 7693A ALS to the Other GC Inlet 61 Check your w
19. Sample vial positions match the run parameters Each solvent bottle contains 4 5 mL of fresh solvent Waste bottles are empty Syringe door is closed Sufficient solvent and waste capacity is available for sample vials Syringe is new or clean Syringe design and size are correct Plunger is secure in plunger carrier loop Needle is aligned with septum retainer nut Syringe is rinsed with solvent GC inlet liner is clean and deactivated GC inlet liner or insert is correct type for injection technique GC inlet septum type is correct GC inlet septum has sufficient life remaining Correct septum nut is installed in GC inlet Operating Guide Operating the 7683 Automatic Sampler 6 To Adapt the 7683 ALS for the COC Inlet 1 Select the on column syringe needed for the column size See Consumables and Parts for the COC Inlet 2 Check the needle to column size See To Check the Needle to Column Size with the COC Inlet 3 Verify that the insert matches the needle size See To Install an Insert on the COC Inlet 4 If necessary replace the septum See To Change a Septum on the COC Inlet 5 Verify that the correct needle support assembly is installed in the injector See To Replace the Needle Support Assembly in a 7683 Injector 250 pm 320 pm 530 pm G2913 60978 standard G2913 60977 6 Install the syringe See To Install a Syringe on the 7683 ALS
20. When the run log contains entries the Run Log LED lights Maintenance log The maintenance log contains entries made by the system when any of the user defined component counters reach a monitored limit The log entry contains a description of the counter its current value the monitored limits and which of its limits has been reached In addition each user task related to the counter is recorded in the log including resetting enabling or disabling monitoring and changing limits or units cycles or duration System event log The system event log records significant events during the GC s operation Some of the events also appear in the run log if they are in effect during a run Operating Guide Agilent 7890A Gas Chromatograph Operating Guide 5 Operating the 7693A Automatic Sampler Placing Vials in the 7693A ALS 50 To Park the Injector 54 To Install a Syringe on the 7693A ALS 55 To Remove the Syringe on the 7693A ALS 58 To Configure Solvent Waste Vials 59 7693A ALS Prerun Checklist 60 To Move the 7693A ALS to the Other GC Inlet 61 To Adapt the 7693A ALS for the COC Inlet 63 This section describes the basic operation of the 7693A automatic liquid sampler ALS For complete information refer to the Agilent 7693A Automatic Liquid Sampler Installation Operation and Maintenance manual Phe Agilent Technologies 49 5 Operating the 7693A Automatic Sampler Placing Vials in the 7693A ALS 50 Two t
21. d Enter the number of the method to store 1 through 9 Press On Yes to store the method and replace the active method Alternatively press Off No to return to the stored methods list without storing the method To manually inject a sample with a syringe and start a run oa A w N Prepare the sample syringe for injection Load the desired method See To load a method Press Prep Run Wait for STATUS Ready for Injection to be displayed Insert the syringe needle into the inlet and puncture the septum Simultaneously depress the syringe plunger to inject the sample and press Start To run a method to process a single ALS sample Prepare the sample for injection Load the sample vial into the assigned location in the ALS tray or turret Load the desired method See To load a method Press Start on the GC keypad to initiate the ALS syringe cleaning sample loading and sample injection method Operating Guide Running a Method or a Sequence from the Keypad 3 After the sample is loaded into the syringe the sample is automatically injected when the GC reaches the ready state To abort a method 1 Press Stop 2 When you are ready to resume running analyses load the appropriate sequence or method See To load a method or To load a sequence Operating Guide 33 3 Running a Method or a Sequence from the Keypad Loading Storing and Running Sequences from the Keypad A sequence can s
22. d Sequence Development Operating Guide Data compiled from samples the output of the detectors is digitized and can be sent to an automated data analysis system such as the Agilent ChemStation where it is analyzed and the results summarized in reports The Agilent ChemStation also can be used to create and store methods and sequences that are sent to the GC through a network 67 7 About Methods Sequences and Data Analysis 68 Operating Guide
23. e Key 38 The GC Component Keys 39 The Status Key 40 The Info Key 41 The General Data Entry Keys 42 The Supporting Keys 43 Method Storage and Automation Keys 44 Keypad Functionality When the GC Is Controlled by an Agilent Data System 45 About GC Status 46 About Logs 48 This section describes the basic operation of the Agilent 7890A GC keypad For additional information on keypad functionality see the Advanced User Guide Phe Agilent Technologies 37 4 Keypad Operation The Run Keys These keys are used to start stop and prepare the GC to run a sample Prep Run Start Stop The Service Mode Key 38 Activates processes required to bring the GC to the starting condition dictated by the method such as turning off the inlet purge flow for a splitless injection or restoring normal flow from gas saver mode See the Advanced User Guide for details Starts a run after manually injecting a sample When you are using an automatic liquid sampler or gas sampling valve the run is automatically activated at the appropriate time Immediately terminates the run If the GC is in the middle of a run the data from that run may be lost Refer to the Advanced User Guide for information on how to restart the GC after pressing Stop Service Mode Is used to access maintenance functions and settings service counters and diagnostics for the GC See the Advanced User Guide for details Operat
24. e displayed information Operating Guide Operating Guide Status lights Introduction 1 The status lights provide a basic look at what is currently happening inside the Agilent 7890A GC Not e Haaay a Run Oven Ramp Remote L o Clock e Run Table Log Gas Service Saver e Due A lit LED on the status board indicates The current progress of a run Pre Run Post Run and Run Items that may require attention Rate Not Ready Service Due and Run Log The GC is controlled by an Agilent data system Remote The GC is programmed for events to occur at specified times Clock Table The GC is in gas saver mode Gas Saver Beeping instrument A series of warning beeps sound if the GC encounters a problem For example a series of beeps sound if the front inlet gas flow cannot reach setpoint The message Front inlet flow shutdown is briefly displayed The flow shuts down after 2 minutes Press Clear to stop the beeping A continuous beep sounds if a hydrogen flow is shut down or a thermal shutdown occurs Press Clear to stop the beep One beep sounds for other types of faults warnings and shutdowns A single beep means that a problem exists but the problem will not prevent the GC from executing the run The GC will emit one beep and display a message The GC can start the run and the warning disappears when a run starts 1 20 Introduction Fault messages indicate hardware problems that
25. eat resistant gloves to protect your hands When the GC is cool remove the column from the oven and cap both ends to keep out contaminants Cap the inlet and detector column fittings and all GC external fittings 29 2 Operating Basics 30 Operating Guide Agilent 7890A Gas Chromatograph Operating Guide 3 Running a Method or a Sequence from the Keypad Loading Storing and Running Methods from the Keypad 32 Loading Storing and Running Sequences from the Keypad 34 This section explains how to load store and run a method or sequence using the GC keypad without the use of an Agilent data system The keypad can be used to select and run a method or automated sequence stored in the GC and run it In this case the data generated from the run is normally sent to an integrator for the data analysis report For information on creating a method or sequence using keypad entry see the Advanced User Guide ot Agilent Technologies bay 3 Running a Method or a Sequence from the Keypad Loading Storing and Running Methods from the Keypad 32 To load a method To store a method oa A w N Press Load Press Method Enter the number of the method to be loaded 1 through 9 Press On Yes to load the method and replace the active method Alternatively press Off No to return to the stored methods list without loading the method Ensure that the proper parameters are set Press Store Press Metho
26. edle support foot Align the syringe flange with the flange guide and press the syringe into place keeping the needle end in the guide hole of the needle support foot Close the syringe latch by swinging it clockwise until it snaps in place Loosen the plunger screw entirely by turning it counterclockwise until the stop is reached 55 5 56 Operating the 7693A Automatic Sampler CAUTION 10 Slide the plunger carrier down until it is completely over the syringe plunger and tighten the plunger thumb screw until finger tight 11 Manually move the plunger carrier up and down If the syringe plunger does not move along with the carrier repeat the previous steps until installed correctly Be sure the plunger thumb screw is secure and tight If the carrier is not completely attached to the syringe plunger it may become detached after a few injections Repeating this movement can damage the syringe 12 Verify that the needle is inside the guide hole of the needle support foot The needle should be straight and pass freely through the needle guide hole If the needle is bent or is outside the guide hole remove the syringe and reinstall See Figure 7 for a properly installed syringe Syringe latch closed Needle support foot Figure 7 Syringe carriage and needle support with syringe installed Operating Guide Operating the 7693A Automatic Sampler 5 13 Close the injector door 14 Do the following only i
27. er 2 Place up to 150 samples in the three vial racks according to the programmed sequence Figure 2 shows tray loading for a sequence that uses tray positions 6 through 21 D o emo 110 100 First vial in position 6 Last vial in position 21 Figure 2 Loading tray positions 3 When finished loading the tray press P on the tray front panel to move the gantry from the Park position to the Home position To use the sample tray the gantry must be out of the Park position Placing vials in the turret no sample tray You can place up to sixteen vials a solvent A a solvent B and a waste bottle in the standalone turret If you use the transfer turret you can load three sample vials and the solvent and waste bottle capacity increases to 15 Figure 1 Placing vials for sandwich injections The ALS system has the ability to draw liquid from multiple vials to create multilayered or sandwich injections Each layer of sample internal standard or solvent can be separated by a layer of air from 0 to 10 of the syringe size Figure 3 and Figure 4 show examples of 2 and 3 layer sandwich injections Operating Guide Operating Guide Operating the 7693A Automatic Sampler 5 Air gap 1 Air gap 2 I 7 T LAAG HAAA VT LL Layer 1 L1 Layer 2 L2 Figure 3 2 layer sandwich injection Air gap 1 Air gap 2 Air gap 3 Layer 1 L1 Layer 2 L2 Layer 3 L3
28. et until it bottoms 7 Press Start to start the run depress the syringe plunger as quickly as possible and withdraw the needle from the inlet Operating Guide Agilent 7890A Gas Chromatograph Operating Guide 7 About Methods Sequences and Data Analysis What Is a Method 66 What Is Saved ina Method 66 What Happens When You Load a Method 67 What Is a Sequence 67 Automating Data Analysis Method Development and Sequence Development 67 Phe Agilent Technologies 65 7 About Methods Sequences and Data Analysis What Is a Method A method is the group of settings required to accurately analyze a specific sample Since every type of sample reacts differently in the GC some samples require a higher oven temperature others require a lower gas pressure or a different detector a unique method must be created for each specific type of analysis What Is Saved in a Method 66 Some of the settings saved in a method define how the sample will be processed when the method is used Examples of method settings include e The oven temperature program required e The type of carrier gas required e The type of detector to be used e The type of inlet to be used e The type of column to be used e The length of time to process a sample Data analysis and reporting parameters are also stored in a method when it is created on an Agilent data system for example ChemStation These parameters describe how to inte
29. etector an electrical signal proportional to the amount of compound detected is generated This signal is generally sent to a data analysis system such as Agilent ChemStation where it shows up as a peak on a chromatogram The Agilent 7890A GC can accommodate up to three detectors identified as Front Det Back Det and Aux Det A complete selection of detectors FID TCD NPD FPD UECD MSD Triple Quadrupole MS and ICP MS is available The type of detector chosen is based on the type of analysis required Operating Guide 17 1 Introduction The Operating Panel The display The operating panel consists of the display status lights and keypad See Keypad Operation and the Advanced User Guide along with the complete suite of documentation included on the Agilent GC and GC MS Hardware User Information amp Utilities DVD that shipped with your instrument for more detailed information The display shows details of what is currently happening in the Agilent 7890A GC and allows you to make changes to parameters as necessary ACTUAL SETPOINT KAKA Use the scroll keys to view additional lines in the display 18 A blinking asterisk prompts you to press Enter to store a value or Clear to abort the entry You cannot perform any other task until this is done Refer to The keypad and to Keypad Operation in this document or to the Advanced User Guide for more details on how to interact with th
30. f the injector tower was removed from the mounting post during installation a If necessary plug in the injector cable b Install the injector on the mounting post c If you have a sample tray calibrate the ALS system Operating Guide 57 5 Operating the 7693A Automatic Sampler To Remove the Syringe on the 7693A ALS To remove a syringe 1 Unplug the injector cable and if desired mount the injector on a parking post 2 Open the injector door 3 Slide the syringe carriage to the top position 4 Completely loosen the plunger thumb screw until it reaches the stop and lift the plunger carrier off of the syringe plunger 5 Open the syringe latch by swinging it in a counterclockwise direction CAUTION Be careful not to bend the syringe needle Only pull the syringe out of the carriage until clear The needle bends easily when still seated in the needle support guide 6 Carefully pull the top of the syringe out of the flange guide then lift the needle out of the needle support foot To install a syringe see To Install a Syringe on the 7693A ALS for details 58 Operating Guide Operating the 7693A Automatic Sampler 5 To Configure Solvent Waste Vials Press Config Front Injector or Config Back Injector on the GC keypad to display the front or back injector configuration parameters CONFIGURE FRONT INJECTOR Wash Mode Bottles 1A 1B Syringe Size 10 0 Wash Mode Bottles
31. following tasks Setting up the GC hardware for an analytical method Starting up the GC See To Start Up the GC Preparing the automatic liquid sampler Install the method defined syringe configure solvent and waste bottle usage and syringe size and prepare and load solvent waste and sample vials For the 7693A ALS see its Installation Operation and Maintenance manual For the 7683 ALS see Operating the 7683 Automatic Sampler Loading the analytical method or sequence into the GC control system See the Agilent data system documentation For standalone GC operation see To load a method and To load a sequence Running the method or sequence See the Agilent data system documentation For standalone GC operation see To manually inject a sample with a syringe and start a run To run a method to process a single ALS sample and To start running a sequence Monitoring sample runs from the GC control panel or the Agilent data system program See About GC Status or the Agilent data system documentation Shutting down the GC See To Shut Down the GC for Less Than a Week or To Shut Down the GC for More Than a Week Operating Guide Instrument Control Operating Guide Operating Basics 2 The Agilent 7890A GC is typically controlled by an attached data system such as Agilent ChemStation Alternately the GC can be controlled entirely from its keypad with outp
32. hooting manual for details One beep sounds when a problem exists but the problem will not prevent the GC from executing the run The GC will emit one beep and display a message The GC can start the run and the warning will disappear when a run starts Fault messages indicate hardware problems that require user intervention Depending on the type of error the GC emits no beep or a single beep Error conditions If a problem occurs a status message appears If the message indicates broken hardware more information may be available Press the applicable component key for example Front Det Oven or Front Inlet Blinking setpoint If the system shuts down a gas flow multiposition valve or the oven Off will blink on the appropriate line of the component s parameter listing If there is a detector pneumatics shutdown or failure in another part of the detector the detector On Off line of the detector s parameter list blinks Operating Guide 47 4 Keypad Operation About Logs 48 Three logs are accessible from the keypad the run log the maintenance log and the system event log To access the logs press Logs to toggle to the desired log The display will indicate the number of entries the log contains Scroll through the list Run log The run log is cleared at the start of each new run During the run any deviations from the planned method including keypad intervention are listed in the run log table
33. ing Guide The GC Component Keys Operating Guide Keypad Operation 4 These keys are used to set the temperature pressure flow velocity and other method operating parameters To display the current settings press any one of these keys More than three lines of information may be available Use the scroll keys to view additional lines if necessary To change settings scroll to the line of interest enter the change and press Enter For context sensitive help press Info For example if you press Info on a setpoint entry the help provided would be similar to Enter a value between 0 and 350 Oven Front Inlet Back Inlet Col 1 Col 2 Aux Col Front Det Back Det Aux Det Analog Out 1 Analog Out 2 Front Injector Back Injector Valve Aux Temp Aux EPC Column Comp Sets oven temperatures both isothermal and temperature programmed Controls inlet operating parameters Controls column pressure flow or velocity Can set pressure or flow ramps Controls detector operating parameters Assigns a signal to the analog output The analog output is located on the back of the GC Edits injector control parameters such as injection volumes and sample and solvent washes Allows for configuration or control of the gas sampling valve GSV and or switching valves 1 to 8 on or off Sets multiposition valve position Controls extra temperature zone
34. injector simply alternates use between the two solvent B vials This mode allows the number of solvent B washes to be twice that of the solvent A washes Operating Guide Operating the 7683 Automatic Sampler 6 To Load Vials in the 7683 ALS Turret 1 Load waste and solvent bottles in the appropriate positions Be sure that there is a waste bottle in each position of the turret regardless of which ones are configured in the method 2 Load the sample vial s in the appropriate locations Positions 1 through 8 for an 8 sample turret and Position 1 for a I sample turret when not using a tray To Load Vials in the 7683 ALS Sampler Tray 1 Install the 1 sample turret if not already installed See To Change the 7683 ALS Turret 2 Place up to 100 sample vials in the four tray quadrants according to the programmed sequence Do not place a sample in the turret Tray loading for a sequence that uses tray positions 6 through 21 is shown below First vial in position 6 Last vial in position 21 3 Review the 7683 ALS Prerun Checklist before starting a run Operating Guide 77 6 Operating the 7683 Automatic Sampler 7683 ALS Prerun Checklist Use this checklist before running analyses to make sure that the sample vials and sampler are ready o u o o u u o u o m u u o u UO OD 78 Sample vials are at least half full Vial cap is centered with no wrinkles and the septum is flat
35. l need to do both Operating Guide Operating Basics 2 To Start Up the GC Successful operation begins with a properly installed and maintained GC The utility requirements for gases power supply venting of hazardous chemicals and required operational clearances around the GC are detailed in the Site Preparation Checklist 1 Check gas source pressures For required pressures see the Agilent GC MSD and ALS Site Preparation Checklist 2 Turn on the carrier and detector gases at their sources and open the local shutoff valves 3 Turn on the cryo coolant at its source if used 4 Turn on the GC power Wait for Power on successful to be displayed 5 If removed install the column 6 Check that the column fittings are leak free 7 Load the analytical method 8 Wait for the detector s to stabilize before acquiring data The time required for the detector to reach a stable condition depends on whether the detector was turned off or its temperature was reduced while the detector remained powered Detector type Stabilization time starting Stabilization time starting from a reduced temperature from detector off hours hours FID 2 4 TCD 2 4 ECD 4 18 to 24 FPD 2 12 NPD 4 18 to 24 Operating Guide 21 2 Operating Basics To Shut Down the GC for Less Than a Week 1 Wait for the current run to finish 2 If the active method has been modified save the changes WARNING Never leave flammable gas flows o
36. matically detectable by the GC but are essential to running a method such as column dimensions carrier and detector gas types makeup gas configurations sample tray settings and column plumbing to inlets and detectors These settings are part of and are stored with the method To view the current configuration for a component such as the inlet or detector press Config then the component key of interest For example Config Front Det opens front detector configuration parameters 43 4 Keypad Operation Method Storage and Automation Keys Load Method Store Seq Run Table Clock Table Seq Control These keys are for loading and storing methods and sequences locally on your GC They cannot be used to access methods and sequences stored by your Agilent ChemStation Are used to load and store methods and sequences on your GC To load a method press Load Method and select one from the list of methods stored in the GC See the Advanced User Guide for more details on these operations Is used to program special events you require during a run A special event could be switching a valve for example See the Advanced User Guide for details Is used to program events to occur at a time of day as opposed to during a specific run This could for example be used to start a shutdown run at 5 00 p m every day See the Advanced User Guide for details on this function Starts stops pauses
37. n if the GC will be unmonitored If a leak develops the gas could create a fire or explosion hazard 3 Turn off all gases except the carrier gas at their sources Leave the carrier gas on to protect the column from atmospheric contamination 4 If you are using cryogenic cooling turn off the cryo coolant at the gas source 5 Reduce detector inlet and column temperatures to between 150 and 200 C If desired the detector can be turned off See the following table to determine if it is advantageous to shut down the detector for a short time period The time required to return the detector to a stable condition is a factor Detector type Stabilization time starting Stabilization time starting from a reduced temperature from detector off hours hours FID 2 4 TCD 2 4 ECD 4 18 to 24 FPD 2 12 NPD 4 18 to 24 28 Operating Guide Operating Basics 2 To Shut Down the GC for More Than a Week Operating Guide 1 2 3 4 Load a GC maintenance method and wait for the GC to become ready For more information about creating maintenance methods see the Maintaining Your GC manual If a maintenance method is not available set all heated zones to 40 C Turn off the main power switch Shut off all gas valves at the gas source If you are using cryogenic cooling shut off the cryo coolant valve at the source Be careful The oven inlet and or detector may be hot enough to cause burns If they are hot wear h
38. on column programmed temperature vaporization and volatiles interface are available The type of inlet chosen is based on the type of analysis being done the type of sample being analyzed and the column being used MULULULUNAN Samples can be injected into the inlets by hand using a syringe or an automatic sampling device such as an Agilent Automatic Liquid Sampler or Agilent Headspace Sampler 12 Operating Guide Introduction 1 Automatic injectors The optional Agilent 7693A automatic liquid sampler ALS with a sample tray and bar code reader automates liquid sample processing The modular design allows the autoinjector to be easily moved from one inlet to another or from one GC to another The modular design also permits easy inlet maintenance The Agilent 7890A GC can accommodate up to two autoinjectors identified as Front Injector and Back Injector Autoinjector LE Sample Tray Bar code reader mixer heater not visible Automatic gas sampling valves The sampling valves are simple mechanical devices that introduce a sample of fixed size into the carrier gas stream Valves are most frequently used to sample gases or liquids in constantly flowing streams The Agilent 7890A GC can accommodate up to two gas sampling valves identified as Valve 1 and Valve 2 Operating Guide 13 1 Introduction The valves are located inside the gas sampling valve box Gas Sampling Valve box 14
39. ork 62 Impact on your datasystem 62 To Adapt the 7693A ALS for the COC Inlet 63 6 Operating the 7683 Automatic Sampler 7683 ALS Turret Types 66 To Change the 7683 ALS Turret 67 To Move the 7683 ALS to the Other GC Inlet 69 To Park the Injector 71 To Install a Syringe on the 7683 ALS 72 To Remove the Syringe on the 7683 ALS 74 To Configure Solvent Waste Vials 75 To Load Vials in the 7683 ALS Turret 77 To Load Vials in the 7683 ALS Sampler Tray 77 7683 ALS Prerun Checklist 78 To Adapt the 7683 ALS for the COC Inlet 79 To Install a 200 um Column With the COC Inlet 80 To Manually Inject Onto a 200 um Column With the COC Inlet 80 7 About Methods Sequences and Data Analysis What Is a Method 82 What Is Saved ina Method 82 What Happens When You Load a Method 83 What Is a Sequence 83 Automating Data Analysis Method Development and Sequence Development 83 Operating Guide Operating Guide Agilent 7890A Gas Chromatograph Operating Guide 1 Introduction Where to Find Information 8 Chromatography UsingaGC 10 The Front View of the Agilent 7890A GC 11 The Back View of the Agilent 7890A GC 11 The Inlets 12 The GC Column and Oven 15 Capillary Flow Technology 16 Detectors 17 The Operating Panel 18 This document provides an overview of the individual components that make up the Agilent 7890A Gas Chromatograph GC Phe Agilent Technologies 1 Introduction Where to Find Information In addition to this document
40. pecify up to five subsequences to be run as well as priority ALS only and post run sequences if defined Each sequence is stored as a number from 1 to 9 To load a sequence 1 Press Load Seq 2 Enter the number of the sequence to be loaded 3 Press On Yes to load the sequence or 0ff No to cancel the load An error message is displayed if the specified sequence number has not been stored To store a sequence 1 Press Store Seq 2 Enter the number of the sequence to be stored 3 Press On Yes to store the sequence or Off No to cancel the store To start running a sequence 1 Load the sequence See To load a sequence 2 Press Seq Control 3 Verify the status of the sequence Running the sequence is running Ready wait the instrument is not ready due to oven temperature equilibration times etc Paused the sequence is paused Stopped proceed to step 4 Aborted the sequence stopped without waiting for the run to finish See Aborting a sequence No sequence the sequence is off or not defined 4 Scroll to the Start sequence line and press Enter to change the status to Running 34 Operating Guide Running a Method or a Sequence fromthe Keypad 3 The Run LED will light and stay lit until the sequence is completed The sequence continues to run until all subsequences are executed or until the sequence is aborted To pause a running sequence 1 Press Seq Control 2 Scroll to Pa
41. rpret the chromatogram generated by the sample and what type of report to print See the Advanced User Guide for more details on what can be included in a method Operating Guide About Methods Sequences and Data Analysis 7 What Happens When You Load a Method What Is a Sequence There are two kinds of methods e The active method This is sometimes referred to as the current method The settings defined in this method are the settings the GC is currently maintaining e User stored methods Up to 20 methods can be stored in the GC When a method is loaded from the GC or Agilent data system the setpoints of the active method are immediately replaced with the setpoints of the method loaded e The method loaded becomes the active current method e The Not Ready light will stay lit until the GC reaches all of the settings specified by the method that was just loaded Refer to Running a Method or a Sequence from the Keypad for details on using the keypad to load modify and save methods A sequence is a list of samples to be analyzed along with the method to be used for each analysis Once defined the sequence may run unattended automatically processing the samples defined in the sequence Refer to Running a Method or a Sequence from the Keypad and the Advanced User Guide for details on how to create load modify and save sequences using the keypad Automating Data Analysis Method Development an
42. s such as a heated valve box a mass selective detector an atomic emission detector transfer line or an unknown device Can be used for temperature programming Provides auxiliary pneumatics to an inlet detector or other device Can be used for pressure programming Creates a column compensation profile 39 4 Keypad Operation The Status Key 40 Status Toggles between setpoint actual values for most commonly reviewed parameters and displays ready D not ready and fault information When the Not Ready status light is blinking a fault has occurred Press Status to see which parameters are not ready and what fault has occurred The order in which items appear in the scrolling display window for Status can be modified You may for example want to display the things you most frequently check in the top three lines so that you do not need to scroll to see them To change the order of the Status display 1 2 Press Config Status Scroll to the setpoint you want to appear first and press Enter This setpoint will now appear at the top of the list Scroll to the setpoint you want to appear second and press Enter This setpoint will now be the second item on the list Continue as above until the list is in the order you require Operating Guide Keypad Operation 4 The Info Key Info Provides help for the currently shown parameter For example if Oven Temp
43. stalling a back injector make sure the cable is available Front injector cable Front injector cable routed through Back injector cable mounting bracket support Figure 8 Front injector cable routed through mounting bracket support Operating Guide 61 5 62 Operating the 7693A Automatic Sampler 6 If the injector cable is disconnected reconnect it to the injector cable port Injector cable port 7 Position the injector on the desired mounting post and inlet cover support foot Check your work The injector must be vertical and stable If the injector will not sit upright on the GC check that the plumbing and cabling under the inlet cover are properly routed in their channels If a tray is installed also verify that the front injector cable is routed correctly through the GC mounting bracket support as shown in Figure 8 If using a tray you should calibrate the system Refer to the 7693A ALS Installation Operation and Maintenance manual Impact on your data system After moving the injector an Agilent data system will detect the configuration change and may prompt you to verify the loaded method Verify the data system will use the new injection source for any runs Operating Guide Operating the 7693A Automatic Sampler 5 To Adapt the 7693A ALS for the COC Inlet The 7693A injectors can inject samples directly into 250 um 320 um and 530 um columns in GCs with a cool on column inlet
44. swing freely 11 Pull down the syringe carriage until the needle support guide tip is near the top of the inlet septum nut The needle support guide should be centered over the hole in the septum retainer nut Make sure that the needle will hit the septum without rubbing on the nut 12 From the GC keypad configure the injector with the installed syringe size a Press Config Front Injector or Config Back Injector b Scroll to Syringe Size and enter the size of the installed syringe Operating Guide 73 6 Operating the 7683 Automatic Sampler To Remove the Syringe on the 7683 ALS CAUTION Open the injector door loop off the syringe plunger Open the syringe latch Plunger carrier loop Plunger screw Flange guide Syringe carrier Syringe latch open Syringe clip Needle support foot TIN 2 Loosen the plunger screw and raise the plunger carrier Be careful not to bend the syringe needle Only pull the syringe out of the carriage until clear The needle bends easily when still seated in the needle support guide CAUTION To limit potential needle puncture hazards proper laboratory safety and correct personal protective equipment PPE should be used 74 4 Carefully lift the syringe flange out of the flange guide until clear then lift the syringe needle out of the needle support guide Operating Guide Operating the 7683 Automatic Sampler 6 To Configure Solvent Was
45. te Vials Operating Guide 1 Press Config Front Injector or Config Back Injector to display the parameters 2 Scroll to the desired parameter detailed below T 1 CONFIGURE FRONT INJECTOR Front Tower INJ1 lt Wash Mode 2 A 2 B syringe size 10 0 ee eae nS a J Front Tower or Back Tower Press Mode Type to select the correct injector connection The example above shows that the front injector tower is connected to the SAMPLER1 INJ1 connector on the back of the GC Wash Mode Press Mode Type to set the wash mode when using a sampling tray The wash modes allow you to use extra solvent A and solvent B wash vials to extend the number of samples you can run before refilling the vials The choices are 3 A 3 B Use 3 wash A and 3 wash B solvent vials alternating between them 1 A 1 B Use 1 wash A and 1 wash B solvent vial 2 A 2 B Use 2 wash A and 2 wash B solvent vials alternating between them When running a sequence with wash mode 3 A 3 B for example the injector will use wash vial positions A and B for the first sample wash vial positions A2 and B2 for the second sample and wash vial positions A3 and B3 for the third sample For the fourth sample the injector returns to use wash vials A and B See 7683 ALS Turret Types for a diagram that explains the wash vial positions in the turret If a solvent wash is not used for the current sample it is skipped For example
46. the injector cable to the right through the channel in the front of the tray and under the cable holder 8 Verify the following for proper installation The injector is vertical If the injector does not sit upright on the GC check that the plumbing and cabling under the inlet cover are properly routed in their channels The alignment pin is properly seated in the alignment hole The injector feet touch the inlet cover 9 Plug the injector into the GC back panel For the front inlet injector location use the connector labeled sampler 1 For the back inlet injector location use the connector labeled sampler 2 Operating Guide Operating the 7683 Automatic Sampler 6 To Park the Injector The parking post for the injector has been replaced by a new design which is compatible with former designs e Former parking post 05890 20795 not compatible with 7890A e New parking post G4513 20562 compatible with 7890A and previous GCs To park the injector simply place it on an installed post Operating Guide 71 Operating the 7683 Automatic Sampler To Install a Syringe on the 7683 ALS 72 Plunger carrier loop j f Open the injector door Remove the syringe if necessary See To Remove the Syringe on the 7683 ALS Slide the syringe carriage up or down Pass the syringe needle through the hole in the needle support foot Align the syringe barrel with the flange guide and syringe clip and press
47. uring a column use these keys to identify the inlet and detector to which the column is attached Delete Removes methods sequences run table entries and clock table entries Delete also aborts the adjust offset process for nitrogen phosphorus detectors NPD without interrupting other detector parameters See the Advanced User Guide for more details 42 Operating Guide The Supporting Keys Operating Guide Time Post Run Logs Options Config Keypad Operation 4 Displays the current date and time on the first line The two middle lines show the time between runs the elapsed time and time remaining during a run and the last run time and post time during a post run The last line always displays a stopwatch While on the stopwatch line press Clear to set the clock to zero and Enter to start or stop the stopwatch Is used to program the GC to do something after a run such as bakeout or backflush a column See the Advanced User Guide for details Toggles between three logs the Run Log the Maintenance Log and the System Event Log The information in these logs can be used to support Good Laboratory Practices GLP standards Accesses the instrument parameters setup option such as keypad and display and diagnostics Scroll to the desired line and press Enter to access the associated entries See the Advanced User Guide for details Is used to set up components that are not auto
48. urret 6 Replace the knurled nut and firmly finger tighten Operating Guide 67 6 Operating the 7683 Automatic Sampler 7 Close the injector door The injector will verify the turret type if the injector is on If the Fault light comes on the turret is not installed correctly 68 Operating Guide Operating the 7683 Automatic Sampler 6 To Move the 7683 ALS to the Other GC Inlet 1 When no runs are scheduled unplug the injector from the back of the GC 2 Lift the injector tower off the mounting post and set it aside CAUTION Use a flathead screwdriver that snugly fits the slot in the top of the mounting post An undersized blade can damage the post top and prevent the injector from mounting properly 3 Remove the mounting post from the inlet cover Install it at the other location The post must be tightened completely Back Front 4 Line up the hole in the base of the injector with the mounting post Lower the injector about 1 inch 2 5 cm onto the post Operating Guide 69 70 Operating the 7683 Automatic Sampler Alignment pin Hole Mounting post Alignment hole Inlet cover 5 Turn the injector so that the turret faces either The front of the GC for front location The left side of the GC for back location 6 Lower the injector until the alignment pin in the base enters the alignment hole in the inlet cover 7 If using a tray with the turret in the front location route
49. urrets are provided with the G4513A injector Figure 1 The 16 sample standalone turret and the 3 sample transfer turret Standalone turret Transfer turret 16 samples 3 samples Top views Figure 1 Injector turrets The labeled positions are defined in Table 1 and Table 2 Table 1 Standalone turret labels Position Label Bottle Vial 1 through 14 1 through 14 Sample vials 15 15 Sample vial L2 Layer 2 bottle 16 16 Sample vial L3 Layer 3 bottle 17 W Waste bottle 18 B Solvent B bottle 19 A Solvent A bottle Operating Guide Table 2 Transfer turret labels Operating the 7693A Automatic Sampler 5 Position Label Bottle Vial 1 L1 Dedicated vial transfer position A Layer 1 bottle 2 L2 Configurable vial transfer position ie 2 bottle 3 L3 Configurable vial transfer position m 3 bottle 4and5 WB Waste B1 B2 bottles 6 through 8 WA Waste A1 A3 bottles 9 through 12 B B4 Solvent B1 B4 bottles 13 through 18 A A6 Solvent A1 A6 bottles Placing vials in the sample tray You must use the transfer turret 1 Park the sample tray by pushing the P button on the sample tray front panel Fault Align Ready The gantry will move to the far left position away from the tray bracket and gripper jaw will move to the far back position away from the tray front panel This allows for clear access to the tray base Operating Guide 51 5 52 Operating the 7693A Automatic Sampl
50. use sequence and press Enter The sequence stops when the current sample run is complete To resume a paused sequence 1 Press Seq Control 2 Scroll to Resume sequence and press Enter The sequence resumes with the next sample To stop a running sequence 1 Press Seq Control 2 Scroll to Stop sequence and press Enter The sequence stops at the end of the currently running subsequence unless Seq gt Repeat sequence is On The sampler tray halts immediately To resume a stopped sequence 1 Press Seq Control 2 Scroll to Resume sequence and press Enter The sequence restarts from the beginning of the sequence Aborting a sequence When a sequence is aborted it stops immediately without waiting for the current run to finish The following will cause a sequence to abort The Stop key is pressed e A sampler error occurs producing an error message e The GC detects a configuration mismatch during a method load Operating Guide 35 3 Running a Method or a Sequence from the Keypad e A running sequence tries to load a method that doesn t exist e The sampler is turned off To resume an aborted sequence 1 Correct the problem See Aborting a sequence 2 Press Seq Control 3 Scroll to Resume sequence and press Enter The aborted sample run will be repeated 36 Operating Guide Agilent 7890A Gas Chromatograph Operating Guide 4 Keypad Operation The Run Keys 38 The Service Mod
51. ut data being sent to an attached integrator for report generation Agilent Data System Users Please refer to the online help included in the Agilent Data System for details on how to load run or create methods and sequences using the data system Standalone GC Users If you are running your GC without an attached data system for details on loading methods and sequences from the keypad see e To load a method e To load a sequence For details on running methods and sequences from the keypad see e To manually inject a sample with a syringe and start a run e To run a method to process a single ALS sample e To start running a sequence Refer to the Advanced User Guide for details on how to create methods and sequences using the GC keypad 25 2 Operating Basics Correcting Problems 26 If the GC stops operation because of a fault for example a flow module shutdown after running out of carrier gas do the following 1 Use the keyboard or data system to stop the alert tone Click Clear on the keyboard or turn off the offending component in the data system 2 Resolve the problem for example by changing gas cylinders or fixing the leak See the Troubleshooting Guide for details 3 Once the problem is fixed you may need to either power cycle the instrument or use the software keyboard or data system to turn the problem component off then on again For shutdown errors you wil
52. ve a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met Contents 1 Introduction Where to Find Information 8 Online User Documentation 8 Chromatography UsingaGC 10 The Front View of the Agilent 7890AGC 11 The Back View of the Agilent 7890A GC 11 The Inlets 12 The GC Column and Oven 15 Capillary Flow Technology 16 Detectors 17 The Operating Panel 18 The display 18 Status lights 19 Beeping instrument 19 Blinking setpoint 20 The keypad 21 2 Operating Basics Overview 24 Instrument Control 25 Correcting Problems 26 To Start Up the GC 27 To Shut Down the GC for Less ThanaWeek 28 To Shut Down the GC for More ThanaWeek 29 3 Running a Method or a Sequence from the
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