Manual & Automatic Syringe Selector Manual
Contents
1. IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSE QUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or con sequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts_01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials author ization number has been issued by our customer service department The customer is respon sible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer2. a complete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human patients CAUTION NOT FOR CLINICAL USE ON HUMAN PATIENTS Harvard Apparatus Syringe Selector l Specifications Syringe Selector Manual Specifications Number of Syringes that can be switched Port Diameter Dead Volume Selected Side Waste Side Connection for the Port Four 0 020 inch 10 ul 30 ul Valco type ZDV connectors Harvard Apparatus Syringe Selector l Introduction Harvard Syringe Selectors are designed to switch the perfusion medium from one of up to four syringes The selected stream flows through a port connected to the animal while all non select ed syringes flow to a common waste port Up to 4 syringes may be connected to the Syringe Selector If less than 4 ports are required the unused ports may be plugged The Automatic Syringe Selector is available in 110 VAC and 220 VAC models Please check to insure that both power supply and the model match The Automatic Syringe Selector is supplied with a computer interface external control remote control and with a manual event control capacity Using the computer interface it can be used in a fully automated manner Theory of Operation Manual Use of the Automatic Syringe Selector 1 Plug the Syringe Selector into an appropriate power outlet and turn the power switch on The LED indicator light on t
3. 0 pl Therefore it should be ensured that all the reducing adapters are fully inserted Precautions should be taken to insure proper flow switching that the flow should equilibriate before switching Usually a 5 to 10 minute wait is sufficient after connecting the new syringe for flow equilibration A higher than expected time may be observed for fluid to reach the animal after switching the syringe if there is a pressure buildup in the system After the initial setup this can be tested by using carbachol in the switched perfusion medium which induces a behavioral seizure as soon as it reaches the probe If the time interval for the fluid is more than expected pressurizing the waste port connecting an equal length of the same connecting tube as the selected outlet side on the waste port would eliminate this problem Controlling the Syringe Selector with a Computer The easiest way of achieving a completely automatic operation is by using a Serial Interfacing Device optional which allows control of up to two fluid selectors and up to four Valco type electrically actuated HPLC valves using a PC However there are alternatives to the PC control of the device The actuator part can be oper ated with many kind of relays open collector transistors BCD or logic level signal outputs or an RS 232 connector If you wish to control the Syringe Selector with other means than manual or the serial interfac ing device please request the technical no
4. Manual amp Automatic Syringe Selector User s Manual Manual Syringe Selector 59 7357 Automatic Syringe Selector 59 7358 Automatic Syringe Selector 59 7359 Harvard Apparatus Syringe Selector l Table of Contents SUBJECT PAGE NO TVEN 1 General Information Warranty and Repairs Lugagnsadnmsdemnjanneiuje 2 NSC UCR OTIS NER SS SN aa 3 TM OCU CUON earar anna eE E E E E E E ESEE 4 NNN 4 COMMECUIONS Gediteeniseicesscvalcndsvelnesiateaieein enn eas Seen T aE ha Saeed MeN dan 5 TTS sosisini indinin inaa a ideae NEEESE 6 GE EE EE pind siaiemsbcxeseaaheetee papers 7 MA aoee E E E E E 7 ks Dead N N raide 8 Controlling the Syringe Selector with a Computer s 8 Harvard Apparatus Syringe Selector l General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial num bers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay cal ibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser
5. he front panel will come on indicating the position of the valve Press the control toggle switch momentarily toward HOME If the valve is not in position 1 it will realign to the position 1 If it is already aligned it will not move As the valve moves towards HOME position 1 it may sequence through each position allowing the possibility that sample may flow briefly through each syringe into the per fusion outlet Care must be taken to prevent possible contamination The best way to avoid this is to always perform operation 2 before before making any connections Make connections as required See Connections Section To switch from one perfusion medium to the next move the toggle switch momentarily toward STEP and the valve will advance from position 1 to position 2 Pressing this switch again will advance it one more step Port 1 is located at the 12 O Clock position The valve rotates counter clockwise Therefore the 9 O Clock position is the port 2 where syringe 2 must be connected Ports 1 2 3 and 4 are 90 apart The drain port is nearest to the actuator handle motor The outlet is nearest to the spring Harvard Apparatus Syringe Selector l Connections In order to insure flexibility the Syringe Selector is designed with ports which accommodate the standard 1 16 inch tubing Any 0 300 inch or longer Valco type nut and ferrule assembly can be used to attach 1 16 inch tubing in the port see Fitting Instructions T
6. his offers endless con nection possibilities using various off the shelf hardware and flexibility in connection materials The following are some suggestions how the Syringe Selector may be connected in a micro dialysis system Using the Standard 23 Gauge Connectors Connections can be made using the supplied 1 16 inch 23 gauge Reducing Adapters The 1 16 inch side is inserted in the port with the ferrule and the nut and using a wrench the ferrule is permanently clamped to the 1 16 inch side A finger tight nut can be used as an alternative to the supplied steel nut and ferrule assembly This makes a removable connector adapter Otherwise the other end of the adapter 23 gauge side can be connected to the CMA blue con nectors to accommodate the Teflon connecting tubes When using the Harvard Fused Silica Tubes a 23 gauge adapter can be used to make a connection Using the PEEK or Stainless Steel 1 16 inch Tubing A 1 16 inch PEEK or Stainless Steel tube may be used for connection The smallest inside diam eter available of these tubes is generally 0 005 inch or 125 um PEEK tubing connections offer chemically inert contact to the passing fluid Further reduction in the inner diameter of these tubes can be achieved by using fused silica connections A 1 16 inch Fused Silica Connector Adaptor can be used to connect to a fused silica connecting tube Other Alternitives Fused Silica Connecting Tubes can be connected directly to the port us
7. ing a Valco fused silica connection adapter However if the switch flow path is not rinsed properly after each used the port may be permanently damaged due to a clogged or broken fused silica adapter in the port Harvard Apparatus Syringe Selector l Fitting Instructions The Valco type Zero Dead Volume Fitting is comprised of four parts a female zero volume fit ting detail a male nut a ferrule and a length of tubing see Figure 1 Since the leak tightness and integrity of the fitting is dependent upon tubing preparation and proper assembly this sec tion addresses those two topics Tube ii TT fi Detail n a ai Cutting and Polishing Since the tubing is an integral part of the fitting it must be properly prepared if the entire fit ting is to function as designed To insure trouble free service Valco suggests using their elec trolytically pre cut and polished tubing in stainless steel electroformed nickel Nickel 200 Hastalloy C or other materials If other tubing is to be used make certain that all tubing ends are cut square with the tube axis and that both the ID and the OD are thoroughly deburred Will Leak Will Not Leak Next inspect the end of the tubing where the ferrule will seat for scratches along its length Figure 2 Visible scratches along the tubing where the ferrule will seat are not acceptable but those behind the front edge of the ferrule will not interfere with the integrity or the fitting M
8. inor scratches can often be eliminated by folding a small piece of fine emery cloth or wet or dry sandpaper 200 to 400 grit around the end of the tubing and rolling the tubing between two fingers This leaves concentric axial lines in the area where the ferrule seats which while not ideal are less likely to cause a leak than longitudinal scratches NOTE Electropolishing is generally not successful as a repair for bad tubing as it ofien simply rounds off the edges of a scratch without removing it Harvard Apparatus Syringe Selector l Fitting Instructions Cleaning After it has been polished the tubing must be cleaned to remove residual metal shavings and grit from the sandpaper This is best accomplished by using a syringe or pipette to force a sol vent such as methyl or isopropyl alcohol or acetone through the tubing and then drying it with clean dry compressed air or carrier gas Fitting Assembly 1 Slide the nut and ferrule onto the tubing in the order shown in Figure 1 2 Insert the assembly into the fitting detail screwing the nut in two or three times by hand 3 Push the tubing all the way forward into the detail so that it seats firmly This is essential for a proper zero dead volume connection 4 Manually turn the nut into the detail until it is finger tight 5 Using the appropriate open end wrench turn the nut 1 4 turn 90 past the point where the ferrule first starts to grab the tubing Fittings larger
9. tes on electronic circuitry of the switch
10. than 1 8 inch will require more than 1 4 turn as much as 120 The amount of force required can vary considerably due to the friction between the nut and the threads and the composition and wall thickness of the tub ing being used Because of these variables a torque specification is unreliable 6 Remove the fitting and inspect it When made up properly the ferrule may be free to spin axially on the tubing but should have no lateral movement along the tubing If the ferrule moves laterally reinstall the fitting into the detail and tighten it another 1 8 turn past finger tight 7 Remove reinspect and repeat if necessary Once made up correctly this Zero Dead Volume detail can be remade many times without loss of integrity Approximately 1 8 tun 45 past finger tight is adequate for most fittings If addi tional torque is required increase in increments of 1 16 turn The switching time time required for motor to move one step forward is approximately 1 sec ond No matter what type of connecting tubes are used it is advisable that the dead volume should be kept to a minimum Minimizing the dead volume especially at all the joints would reduce the time it takes for alternative perfusion medium to reach the probe tip Harvard Apparatus Syringe Selector l Calculating Dead Volume The dead volume can be calculated by adding volumes of all the tubes in a flow path A syringe selector has a dead volume of approximately 1
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