RUO Data Sheet for International
Contents
1. EDTA Decloaker 5X Pretreatment Reagent Control Number 902 917 013015 Catalog Number BRR 917 L M Description 100 500 ml concentrate Intended Use For Research Use Only Not for use in diagnostic procedures Summary amp Explanation EDTA Decloaker is a heat retrieval solution pH 8 4 8 7 It is recommended for use with antibodies with low antigen expression cyclin Dl CDla CD3 CD4 CD8 CD23 bcl 6 CD61 CD79a and TdT EDTA Decloaker is a potent agent for epitope retrieval and tissues may be 5 10 times more sensitive to immunohistochemical staining EDTA Decloaker incorporates Assure technology a color coded high temperature pH indicator solution The end user is assured by visual inspection that the solution is at the correct dilution and pH This product is specially formulated for superior pH stability at high temperatures and will help prevent the possiblity of losing pH sensitive antigens EDTA Decloaker is non toxic non flammable odorless and sodium azide and thimerosal free Known Applications Immunohistochemistry formalin fixed paraffin embedded tissues Supplied As 100ml EDTA Decloaker 5X concentrate BRR917L 500ml EDTA Decloaker 5X concentrate BRR917M Materials and Reagents Needed But Not Provided Microscope slides positively charged Desert Chamber Drying oven Positive and negative tissue controls Xylene Could be substituted with xylene substitute Ethanol or reagent alcohol De2. cloaking Chamber Pressure cooker Deionized or distilled water Wash buffer TBS PBS Avidin Biotin Blocking Kit Labeled Streptavidin Kits Only Peroxidase block Protein block Primary antibody Negative control reagents Detection kits Detection components Chromogens Hematoxylin Bluing reagent Mounting medium Biocare Medical Products Refer to a Biocare Medical catalog for further information regarding catalog numbers and ordering information Certain reagents listed above are based on specific application and detection system used Storage and Stability Store at room temperature Do not use after expiration date printed on vial If reagents are stored under conditions other than those specified in the package insert they must be verified by the user Diluted reagents should be used promptly any remaining reagent should be stored at room temperature Biocare Medical LLC 4040 Pike Lane Concord CA 94520 saal Tel 800 799 9499 www biocare net Staining Protocol Recommendations 1 Deparaffinize tissues and hydrate to water If necessary block for endogenous peroxidase and wash in DI water 2 Dilute concentrate EDTA at a ratio of 1 5 1 ml EDTA to 4 ml deionized water 3 Place slides into 1X retrieval solution in a slide container e g Coplin jar Tissue Tek staining dish or metal slide canister 4 Retrieve sections under pressure using Biocare s Decloaking Chamber Follow the recomme
3. egulations for disposal of any toxic substances Proclin is a trademark of Rohm and Haas Company or of its subsidiaries or affiliates Technical Support Contact Biocare s Technical Support at 1 800 542 2002 for questions regarding this product Page 1 of 1 Fax 925 603 8080
4. ndations in the antibody data sheet and Decloaking Chamber User Manual 5 Check solution for appropriate color change See Technical Note 1 6 Gently rinse by gradually adding DI water to the solution then remove slides and rinse with DI water Technical Notes 1 Concentrated EDTA Decloaker is a blue color RTU or 1X solution is a clear blue color When heating if the solution turns greenish blue green or yellow the pH of the solution is not optimum and tissue staining may be significantly reduced The pH of the 1X solution should range from 8 0 to 8 7 for optimum staining The grade of deionized water is very important If high grade water is difficult to obtain sterile water may be used 2 If using Biocare s Desert Chamber Pro a programmable turbo action drying oven dry sections at 25 C overnight or at 37 C for 30 60 minutes and then dry slides at 60 C for 30 minutes 3 Use positive charged slides use Biocare s Kling On HIER Slides and cut tissues at 4 5 microns Do not use any adhesives in the water bath Poor fixation and processing of tissues will cause tissue sections to fall off the slides especially fatty tissues such as breast Tissues should be fixed a minimum of 6 12 hours 4 Protocol time and temperatures for HIER can vary depending on the Decloaking Chamber model used Please refer to the relevant Decloaking Chamber manual for appropriate protocol times and temperatures Limitations This product i
5. s provided for Research Use Only RUO and is not for use in diagnostic procedures Suitability for specific applications may vary and it is the responsibility of the end user to determine the appropriate application for its use Precautions 1 This product is not classified as hazardous The preservative used in this reagent is Proclin 300 and the concentration is less than 0 25 Overexposure to Proclin 300 can cause skin and eye irritation and irritation to mucous membranes and upper respiratory tract The concentration of Proclin 300 in this product does not meet the OSHA criteria for a hazardous substance Wear disposable gloves when handling reagents 2 Specimens before and after fixation and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens If reagents or specimens come in contact with sensitive areas wash with copious amounts of water 3 Microbial contamination of reagents may result in an increase in nonspecific Staining 4 Incubation times or temperatures other than those specified may give erroneous results The user must validate any such change 5 Do not use reagent after the expiration date printed on the vial 6 The SDS is available upon request and is located at http biocare net 7 Consult OSHA federal state or local r
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