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1. Submersible Battery Pack Battery Lithium lon Weight in Air 0 75 kg 1 65 Ibs Weight in Water 0 22 kg 0 50 Ibs 13 46 cm 5 3 in Diameter 7 cm 2 75 in Material Delrin 0 600 meters Capacity 2 200 mAH Voltage Output 14 8V nominal See on SLES cir lal overcharge over discharge over heating short 998 2205 Rev K Page 26 amp ulti Sensor Platform Appendix B Recommended Practices Description The Turner Designs C6 Multi Sensor Platform can be used with Cyclops 7 sensors for many different applications It can also be integrated into a multi parameter system It is capable of logging and storing data and uses algorithms to compute direct concentrations of fluorophores Identification Each Cyclops 7 Sensor is identified by a letter stamped into the connector C Chlorophyll U CDOM O Crude Oil F Fluorescein B Optical Brighteners P Phycocyanin E Phycoerythrin R Rhodamine A PTSA T Turbidity and G Refined Oil Fuels Recommended Measurement Practices The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a dark plastic or glass container for your standards and or samples Note Some plastics may fluoresce and might interfere with measurements 2 If using a glass beaker place it on a non reflective surface preferably black 3 Ensure that
2. This cable is an optional accessory for the C6 Itis intended for deployments in stand alone mode Note When using this cable to connect to the battery the battery bracket is not required However use of the battery bracket is recommended when possible as it provides stability for the connection 998 2205 Rev K Page 29 Extender Cables 10 Meter P N 105 2595 shown 25 Meter P N 105 2596 requires P N 2200 900 50 Meter P N 105 2597 requires P N 2200 900 Custom lengths are available upon request These cables are optional accessories for the C6 They allow the C6 to be deployed at a variety of depths Boosters P N 2200 900 The Booster Kit is an optional accessory for the C6 and is required for cable lengths greater than 10 meters The Boosters supply a stable power source and maintain connection with the C6 for deployment depths greater than 10 meters The simple and rugged design allows the Boosters to be used in any aquatic environment 998 2205 Rev K Multi Sensor Platform Connect to extender cable P N 105 2596 or 105 2597 Connects to power source cable P N 2200 150 or 2200 160 or 2200 165 or 2200 170 Connects to instrument Page 30 ulti Sensor Platform Appendix D Wiring Guide The C6 Multi Sensor Platform outputs digital data in a format that can be read by C Soft software or a terminal emulator program It can be integrated into larger multi parameter systems such as C
3. 998 2205 Rev K Page 37 Appendix Installing the Shade Cap Introduction The C6 Multi Sensor Platform s Shade Cap P N 2200 510 offers protection for the Cyclops 7 sensors from damage when deploying recovering or transporting the instrument in fast flowing environments and or from bottoming out in shallow environments during vertical profiling The Shade Cap also helps block other possible sources of light that may interfere with Cyclops 7 readings Installation 1 Use a screwdriver to remove the screws that fasten the C6 Multi Sensor Platform s support bracket P N 130 2210 2 Remove the C6 Multi Sensor Platform s support bracket T Support bracket screws gt 5 Support bracket Support bracket rods 3 Unscrew the 3 stainless steel rods attached to the C6 endcap 4 Position and mate the base of the Shade Cap to the C6 endcap Shade Cap Base C6 Endcap a 998 2205 Rev K Multi Sensor Platform By hand screw the 3 stainless steel rods onto the C6 endcap until they are fully tightened Support bracket rods lt i Reposition and mate the support bracket with the 3 stainless steel rods Refasten the support bracket to the stainless steel rods using the screws provided T lt Support bracket l i screws Support bracket If you have a wiper P N 2200 450 slide the wiper unit through the support bracket and mate the wiper unit with the C6 port aie
4. Computer Interface cable with 12V power supply Integration Interface Adapter Cable see Appendix M and N for more information about integration Sensor fasteners Sd 8 pin female plug CD including C Soft software user s manual cable guide and quick start guide Temperature sensor Sensor fastener Eyelift holes Muiti sensor piatom 8 pin female end plug lt i we 2200 009 URNER 1e Sal 9 2200 809 sic DE Pressure sensor Integration Interface Adapter Cable Computer Interface Cable 998 2205 Rev K Page 5 Sensor Platform Rechargeable Submersible Battery Pack including charger P N 2200 601 see Appendix K for more information 2 2 Optional Accessories Battery Bracket P N 2200 603 see Appendix J for more information Note Required for use with Battery Pack and Analog Output Adaptor C6 Mechanical Wiper P N 2200 450 and Replacement Brushes P N 2200 460 see Appendix G for more information 6 pin male plugs P N 105 2210 Flow Cap P N 2200 700 see Appendix H for more information Shade Cap P N 2200 510 see Appendix for more information gt Solid Secondary Standard SSS for in vivo Chlorophyll Phycocyanin Phycoerythrin Rhodamine and Fluorescein P N 2100 900 see Appendix E for more information Solid Secondary Standard SSS for UV Sensors CDOM Optical Brighteners Refined Fuels and Crude Oil P N 2100 90
5. Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Select Atmospheric and gravity offset Enter the latitude Step 3 Select Save Calibration button Future depth values will be scaled to the offset selected 3 4 Log Setup Screen The Log Setup screen allows the user to configure a logging session set the mechanical wiper to its home position and enable logging The C6 Multi Sensor Platform can log just over 64 000 lines of data Enter a log start date MM DD YYYY and time HH MM SS Enter the sampling interval HH MM SS The minimum sampling interval is 1 second 998 2205 Rev K Page 19 amp ulti Sensor Platform Ifthe mechanical wiper is installed click the Wiper box to enable Select the number of revolutions from the dropdown box 1 10 revolutions All sensors will be wiped at specified revolutions before each measurement Note If users select revolutions that exceed sampling interval a warning message will be displayed Note Do not click the Wiper Box to enable wiper if the wiper is not installed C Soft Manager x The sampling interval is too short for the number of wiper revolutions You must choose a longer sampling time less revolutions or turn the wiper off Sampling Maximum Interval of Revs 1 10 0 1 2 31
6. Tr Multi Sensor Platform 9 Turning clockwise fully hand tighten the Shade Cap housing onto the Shade Cap base connector Shade Cap s lt End Cap Shade Cap lt Housing 10 Turning clockwise thread the Shade Cap s end cap to the Shade Cap housing until it is fully hand tightened Specifications Dimensions Length 30cm 11 81 in Diameter 3 cm 5 118 in Volume Weight 1 042 kg 2 296 Ibs 1 7 L 0 4491 U S gallons 998 2205 Rev K Page 39 Multi Sensor Platform Appendix J Installing the Battery Bracket and Battery Introduction The C6 Battery Bracket P N 2200 603 was designed for easy self contained compact deployment of the C6 Multi Sensor Platform The C6 Multi Sensor Platform s Battery Bracket eliminates the need for a cable connection between the battery and the C6 Multi Sensor Platform The bracket provides balance and stability for the C6 Multi Sensor Platform and submersible battery Benefits Provides balance and stability e Strong durable delrin and stainless steel components Creates a single compact instrument Establishes a direct link between battery and C6 Multi Sensor Platform e Simple design facilitates cleaning of battery Specifications Material Delrin Plastic amp 316 Stainless steel Diameter 4 inches 10 2 cm Length 5 25 inches 13 3 cm Weight 0 62 Ibs 0 28 kg 998 2205 Rev K Installation 1 Remove the 2 eye li
7. end of life recycling program in an environmentally friendly way amp ulti Sensor Platform 1 1 The C6 Multi Sensor Platform was designed to integrate up to six Cyclops 7 submersible sensors for extended or short term deployments The C6 Multi Sensor Platform provides automatic gain control calibration data logging and digital data output for each Cyclops 7 sensor The C6 Multi Sensor Platform comes with factory installed temperature and pressure sensors and is rated to a depth of 600 meters The C Soft Windows based user interface allows for intuitive calibration data logging set up and file downloading Existing Cyclops 7 users can easily integrate their sensors into the C6 Multi Sensor Platform 1 Introduction 1 2 Important Note Regarding Use of Fluorescein Sensor For C7 s manufactured prior to 2 15 2011 the Fluorescein sensor should not be enabled for simultaneous data collection with Chlorophyll and Rhodamine sensors or it will produce unreliable results For C7 s manufactured after 2 15 2011 this interference was designed out If you would like to send your C7 back to Turner Designs to resolve this please contact sales turnerdesigns com 998 2205 Rev K Page 4 ensor Platform 2 Inspection and Setup 2 1 Instrument Checklist The C6 Multi Sensor Platform package P N 2200 000 includes C6 Multi Sensor Platform Factory installed pressure sensor Factory installed temperature sensor
8. The first uses only the atmospheric pressure as an offset This is suitable for any shallow application including most fresh water applications 2 The second compensates for gravity variations with latitude as designated from the UNESCO Technical Papers in Marine Science 44 http unesdoc unesco org images 0005 000598 059832EB pdf This method assumes 0 C and 35PSU salinity ini x Channel Calibration Unit Label Depth Current Pressure 0 00 PSI Step 1 Press Set Zero to set the Zero Reading atmospheric zero Set Zero Step 2 Select a pressure compenstion 3 Atmospheric offset only method Atmospheric and gravity offset Step 3 When you are done press Save Calibration or press Cancel at Save Calibration Cancel any time to abort this calibration Atmospheric Offset Method 1 Step 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero 998 2205 Rev K Page 18 amp ulti Sensor Platform Select Atmospheric offset only Step 2 Step 3 Select Save Calibration button Future depth values will be scaled to the offset selected Atmospheric and Gravity Offset Method 2 Step 1 Select the Calibrate button for depth in the Current Data screen
9. Using your Cyclops 7 Fluorometer measure a sample containing algae and record the response and the gain values for that measurement 2 Dry off the optical end of the Cyclops 7 attach the Solid Secondary Standard to the fluorometer and adjust the Solid Secondary Standard to produce the same response in the same gain as in step 1 3 Perform a chlorophyll extraction to determine the actual chlorophyll concentration of the sample NOTE EPA Method 445 0 in vitro determination of chlorophyll in algae can be found on Turner Designs website 4 The Solid Secondary Standard s signal is now equivalent to the concentration value determined from step 3 and can be used in place of a liquid primary standard for future calibration of that specific Cyclops 7 Fluorometer Use of the Solid Secondary Standard for Dye Tracing Applications The Solid Secondary Standard can also be used to check fluorometer stability when making dye concentration measurements If necessary the Solid Secondary Standard can be used to establish a new correlation voltage without using a calibration solution each time 998 2205 Rev K Multi Sensor Platform 1 Using your Cyclops 7 Fluorometer measure a dye solution with known concentration and record the response as well as the gain values for that measurement 2 Dry off the optical end of the Cyclops 7 attach the Solid Secondary Standard to the fluorometer and adjust the Solid Secondary Standa
10. 00 21 46 06 16 08 10 35 28 78 60 78 36 0 00 21 48 06 16 08 10 35 29 80 44 76 80 0 00 21 48 a i start Chelsea Donova O Manual and Inst W untitled Paint C Soft Manager W7 Microsoft Word E BO gam 13 YU Ze 10 35 am Waveform Screen Users can graphically view real time data for each sensor by clicking the Display Waveform box Sensor identification name and units will be displayed on the Y axis Time and date will be displayed on the X axis Clicking the Autoscale box will automatically scale graphs Users may also rescale graphs by unchecking the Autoscale box and manually entering desired values for either Y or X axis ranges Data can be saved during waveform mode by clicking Start Data Capture Data files will be saved as csv format Rev K Page 13 Multi Sensor Platform File Settings COM Port Help C6 Multi Sensor Platform Autoscale V Settings Current Data Log Setup Download Data j i APAA MAAA An Vf r ae s A My Py JV a 7 Wy ct y Ly at wi iv WW idl VN Current Data t amp Chan 1 Chlorophyll a 15 52 Chan 2 Brighteners 14 28 Calibrate Chan 3 Fluorescein 35 76 Calibrate Chan 4 CDOM 12 8 Chan 5 Phycocyanin 65 64 ls Chan 6 Of 23 6 Depth meters 0 Calbrate Temp c 23 1 View Calibration Data Start Data Capture 6 29 2007 6 29 2007 lt a Calibration Screen Us
11. 40 a 4 T 10 Cancel After log set up is complete select Enable Datalog See section 3 5 Enabling Data Output Ready State A prompt will appear asking if user is ready to enable logging select Yes Note If data logging is set to start for a time in the past there will be a minimum delay of 100 seconds before startup after power has been supplied Otherwise wait until the data logging start time has been reached for data logging to begin WARNING As soon as data logging is enabled users will be unable to conduct calibrations or view current data This should be the last step before the C6 Multi Sensor Platform is deployed 998 2205 Rev K Page 20 sensor Platform 3 5 Enabling Data Output Ready State The Data Output Ready State must be enabled for the C6 Multi Sensor Platform in order to log data capture real time data output and or integrate the C6 with other instrumentation see Appendix M and N for more information about integration There are five necessary steps to ensure the instrument is properly set up for data output 1 Set the instrument s clock to PC or local time in the Settings Tab 2 Select the sensors you expect to use during sampling 3 Calibrate selected sensors for either Direct Concentration Mode or Raw Fluorescence Units Blanked RFUB Mode see section 3 2 2 4 Set the start time sampling and wiping intervals in the Log Setup Tab Note Wiping interval is o
12. Current Data Sensor labels are displayed but cannot be changed in the Current Data screen Real time values are displayed next to each channel and in the adjacent Data Table Acalibration button is available for all Cyclops 7 sensors and the depth sensor Real time data can be saved by selecting the Start Data Capture button Users will be prompted to enter a file name and file location Data files will be saved in csv format 998 2205 Rev K Page 12 998 2205 Multi Sensor Platform Tabular Screen Real time data including date time units and temperature are continuously scrolling on the tabular screen for enabled sensors Each sensor identification name will appear in the associated column headings If units are not selected during calibration a relative fluorescence units RFU label will appear C Soft Manager 18 x File Settings COMPort Window Help Connected s Data Table Display Waveform _ Date Time Rhodamine Phycocyanin Depth Tem Settings Current Data Log Setup Download Data l ae Re ate 4 p ERES 06 16 08 10 34 57 69 08 78 72 0 00 21 47 06 16 08 10 34 58 69 72 76 64 0 00 21 47 06 16 08 10 34 59 70 24 72 20 0 00 21 47 Chan 1 Rhodamine 80 44 __ Calibrate 06 16 08 10 35 00 70
13. 4 see Appendix E for more information Note Solid Secondary Standard not available for turbidity Booster Kit P N 2200 900 l Note Required for cable lengths greater than 10 meters 6 pin male plug Extender Cables 10 meters P N 105 2595 25 meters P N 105 2596 50 meters P N 105 2597 Locking Custom lengths available upon request Booster 1 gt gt Booster 7 24 Pigtail Interface Cable with Locking Sleeve P N 2200 170 Flow Cap z gt fa Submersible battery 998 2205 Rev K Page 6 amp ensor Platform Shade Cap Mechanical wiper Battery bracket 2 3 Installing Cyclops 7 Sensors Up to six Turner Designs Cyclops 7 sensors can be installed onto the C6 Multi Sensor Platform The center port is intended for the optional mechanical wiper only Follow the instructions below to successfully integrate your Cyclops 7 sensor 1 Place the fastener on one of the Cyclops 7 ports of the C6 Multi Sensor Platform and rotate to tighten Make sure not to over tighten 2 Align the 6 pin male end of the Cyclops 7 sensor with the C6 Multi Sensor Platform port and fastener and press in the sensor 3 Hold the Cyclops 7 in place and rotate the remaining ring on the fastener to tighten This will lock the Cyclops 7 into position 4 Follow the same installation instructions for the remaining Cyclops 7 Sensors 5 Install a 6 pin male plug onto any unused port on the C6 Multi Se
14. 40 74 72 0 00 21 48 06 16 08 10 35 01 68 92 74 28 000 21 48 Calibrate 06 16 08 10 35 02 67 92 78 28 0 00 21 47 06 16 08 10 35 03 71 28 73 20 0 00 21 47 Chan 3 Phycocyanin 76 8 Calibrate 06 16 08 10 35 04 71 76 77 04 0 00 21 48 06 16 08 10 35 05 73 88 77 00 0 00 21 47 Calibrate 06 16 08 10 35 06 73 24 76 24 0 00 21 47 06 16 08 10 35 07 72 48 74 00 0 00 21 47 06 16 08 10 35 08 73 76 73 28 0 00 21 47 alibra 06 16 08 10 35 09 74 48 79 60 0 00 21 48 F 06 16 08 10 35 10 76 28 76 64 0 00 21 47 pes 06 16 08 10 35 11 75 68 75 92 0 00 21 47 06 16 08 10 35 12 72 12 72 36 0 00 21 48 Depth meters 0 Calibrate 06 16 08 10 35 13 73 60 74 40 0 00 21 47 06 16 08 10 35 14 72 64 72 64 0 00 21 48 Temp c IE 06 16 08 10 35 15 75 40 71 24 0 00 21 47 06 16 08 10 35 16 74 00 70 80 0 00 21 48 06 16 08 10 35 17 76 44 73 20 0 00 21 48 06 16 08 10 35 18 74 64 78 16 9 00 21 48 06 16 08 10 35 19 76 32 74 24 0 00 21 48 nee 06 16 08 10 35 20 78 08 75 12 0 00 21 48 View Calibration Data 06 16 08 10 35 21 79 04 2 28 0 00 21 48 06 16 08 10 35 22 81 48 74 76 0 00 21 48 06 16 08 10 35 23 70 84 78 52 0 00 21 48 06 16 08 10 35 24 76 16 74 60 0 00 21 48 06 16 08 10 35 25 81 36 73 28 9 00 21 46 Start Data Capture 06 16 08 10 35 26 80 52 72 92 0 00 21 48 06 16 08 10 35 27 80 56 75 68 0
15. 72 6 16 08 15 07 26 01 61 130 83 0 00 23 72 6 16 08 15 07 27 91 23 130 95 0 00 23 72 6 16 08 15 07 28 01 09 130 95 0 00 23 72 6 16 08 15 07 29 01 01 138 92 0 00 23 72 6 16 08 15 07 30 01 23 131 10 0 00 23 72 998 2205 Rev K Page 42 amp ulti Sensor Platform Most applications will integrate streaming data Below are instructions on how to save streaming data on a PC To save data 1 On the HyperTerminal window toolbar choose Transfer and then Capture Text File Edit View Call Transfer Help Pla ele a E EF Send File 3 Receive File Capture Text Send Text File em Capture to Printer 2 When you click on Capture Text a window will appear asking you to name and save the txt file to the location of interest Folder C Documents and File SEA TURNERDESIGNS CAPTURE TT Browse aea 3 Once you are finished you will need to return to the HyperTerminal window toolbar choose Transfer and then Capture Text and select Stop This will stop data capture to the txt file File Edit View Call Transfer Help Send File Receive File mma Send Text File Pause Resume Capture to Printer T 998 2205 Rev K Page 43 amp ulti Sensor Platform Turner Designs offers Integrator Firmware This firmware is designed for use by customers that are integrating their C6 with an external datalogger CTD or similar system The Integrator Firmware is different
16. OBSOLETE Computer Integration Cable P N 2200 160 and 2200 165 This can be identified by the yellow band of heat shrink The integration cable is used to connect the C6 to a PC or data logger for continuous data capture This cable is recommended for serial data output as it prevents interruptions in the C6 data transmission to the PC Replaced by P N 2300 115 yellow heat shrink 12 VDC Power Supply P N 159 0191 This is included as a standard accessory with the C6 The power supply connects to the power connector on the interface or integration cable which will supply power to the C6 998 2205 Rev K Page 28 Multi Sensor Platform 12 VDC Power Supply Car Adaptor P N 2900 151 This is an optional accessory for the C6 This cable connects to the power connector on the interface or integration cable and a car cigarette lighter receptacle or other similar receptacle 24 Pigtail Interface Cable with Locking Sleeve P N 2200 170 This cable is an optional accessory for the C6 It can be used to connect the C6 to a datalogger and or external power source that the user must wire themselves replacing the standard interface and integration cables Extra care should be taken with this cable to make sure all wiring specifications are strictly followed e w oe WR Power ground and V BATT are not common ee RX TX N A N A N A l ME Battery Interface Cable with Locking Sleeves P N 105 2590
17. TDs or buoys refer to Appendix M and N for additional information An 8 pin impulse cable provides two end connections 1 a 9 pin RS232 serial port for connection to a PC or laptop computer and 2 a 12V port for supplying power to the unit C6 Multi Sensor Platform bulkhead and serial port connectors Sub D Connector OOO O Wire Guide ee ee ees eee T Yellow RTS amp CTS PIN 7 8 998 2205 Rev K Page 31 Multi Sensor Platform Appendix E Using the Solid Secondary Standard Introduction The following information describes how to use the Cyclops 7 Solid Secondary Standards P N 2100 900 P N 2100 904 In Vivo Chlorophyll Rhodamine WT Fluorescein Phycocyanin and Phycoerythrin CDOM Crude Oil Refined Fuels and Optical Brighteners Features Can be used in place of a primary liquid standard once a correlation between a primary standard and the solid secondary standard is established Can be used to check fluorometer stability and or check for loss in sensitivity Provides a broad range of very stable fluorescent responses Has an adjustment screw allowing users to set to a desired signal Installation 1 998 2205 Before installing the Solid Secondary Standard ensure that the optical surface of the Cyclops 7 is completely clean and dry Fully mate the Solid Secondary Standard with the optical end of the Cyclops 7 Rotate the Solid Secondary Standard in either d
18. TURNER TS DESIGNS Reliable Instruments for an Unreliable World Multi Sensor Platform User s Manual April 23 2012 P N 998 2205 Revision K TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 ulti Sengor Platform Table of Contents 1 introduction 1 1 Introduction 1 2 Important Note Regarding the Use of the Fluorescein Sensor 2 Inspection and Setup 2 1 Instrument Checklist 2 2 Optional Accessories 2 3 Installing the Cyclops 7 Sensors 3 C Soft Windows User Interface 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 3 1 2 Software Installation 3 1 3 Integration Interface Adapter Cable Installation 3 1 4 PC Software Interface 3 2 Software Operation 3 2 1 Settings Screen 3 2 2 Current Data and Calibration Screens 3 3 Calibration Procedures 3 3 1 Direct Concentration Calibration 3 3 2 Raw Fluorescence Calibration Blank Subtracted 3 3 3 Raw Fluorescence Calibration 3 3 4 Depth Calibration 3 4 Log Setup Screen 3 5 Enabling Data Output Ready State 3 6 Downloading and File Management 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing 4 1 2 Care for the Bulkhead Connector 4 1 3 Care for the Sensor Ports 4 2 Terms 4 3 Warranty Service 4 4 Out of Warranty Service Appendices A Specifications B Recommended Practices C Cable Guide D Wiring Guide Using the Solid Secondary Standard F Linear Range Quenching and Temperatu
19. ace in the cache memory on your computer We recommend you do a Disk Cleanup to see if that solves the problem and allows you to download files Disk Cleanup can be found in the following location on most Windows versions Start Menu gt Programs gt Accessories gt System Tools gt Disk Cleanup After the initial scan of the computer completes customers should select all the boxes and select OK to perform the cleanup 998 2205 Rev K Page 22 ulti Sensor Platform 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing The C6 Multi Sensor Platform should be rinsed or soaked in fresh water following each deployment Care for the bulkhead connector Install the 8 pin female end plug to the bulkhead between uses A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do NOT use WD 40 it will destroy the connectors Care for the sensor ports Install 6 pin male plugs onto any vacant port A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do not use WD 40 It will destroy the connectors 4 2 Warranty Terms Turner Designs warrants the C6 and acc
20. ck the My Computer Icon Note For Windows Vista or 7 users open the start menu and right click on Computer Left click properties to open the properties window Rev K Page 9 998 2205 6 10 ulti Sensor Platform If running Windows XP or older versions of Windows click on the Hardware tab and then click Device Manager to open the list of devices installed on your computer Note For Windows Vista or 7 users the Device Manager link is located in the upper left part of the properties window you will not see a Hardware tab Scroll down and open the Ports heading from the list The newly installed Adapter will be titled USB Serial Port followed by a COM port designation in parentheses for example COM 5 If the USB Serial Ports COM designation is labeled as any number from 1 6 your installation is complete Close all windows and make sure to set C Soft to that specified COM designation when trying to connect via USB Adapter Cable connection If the USB Serial Ports COM designation is greater than 6 right click on the USB Serial Port and click properties which will open up the properties menu From that menu click on the Port Setting tab and then click Advanced which will open the advanced settings menu for that USB Serial Port Choose any COM port 1 6 from the COM port pull down menu and click OK Note Ideally you want to start with any port that i
21. e thread the Flow Cap s endcap to the Flow Cap housing until it is fully hand tightened lt Outflow Valve a i 1 NOTE Turner Designs L recommends the configuration for the Flow Cap It is recommended to position the instrument vertically with the sensor head facing upward to expel any air from the system that might cause skewed readings In Flow Valve Page 36 Multi Sensor Platform Installation with Mechanical Wiper 6 Turning clockwise thread the Flow Cap s endcap 1 Follow the Flow Cap Installation Instructions 1 5 to the Flow Cap housing until it is fully hand located on page 1 tightened 2 Position and mate the wiper bracket P N 130 B l Sumow vave 2215 with the 3 stainless steel rods 5 3 Fasten the wiper bracket to the stainless steel S rods using the screws provided 4 Slide the wiper unit through the wiper bracket and mate the wiper unit with the C6 port Wiper Unit In Flow Valve Bracket Fastening k Screws Calibration Cup Use The In Flow Valve can be unscrewed and replaced with a cap or screw plug when using the Flow Cap as a calibration cup for calibrating using solutions standards Wiper Bracket 5 Turning clockwise fully hand tighten the Flow Cap housing onto the Flow Cap base connector Specifications Black ABS Diameter 3 cm 5 118 in Volume 1 7 L 0 4491 U S gallons Weight 1 042 kg 2 296 Ibs
22. e the fastener on the center port of the C6 Multi Sensor Platform and rotate clockwise to tighten Make sure not to over tighten 2 After aligning the mechanical wiper s 6 pin male end with the C6 Multi Sensor Platform s center port rotate the other end of the fastener clockwise to secure the wiper to the C6 3 Connect the C6 to a computer that has C Soft software and supply power 4 Start the C Soft software 5 Click on the Log Data tab 6 Click the Test Wiper button to bring the motor shaft to its home position 7 Install O ring into motor shaft groove Note Do not lubricate O ring 8 Slide the wiper assembly onto the motor shaft with brushes facing towards the optical head and position the brushes between the Cyclops 7 optical heads 9 Hand tighten nut to secure wiper assembly Note Do not use any tools to tighten the tightening nut 998 2205 Rev K 10 Click test wiper 11 If the wiper does not come to rest in the desired position unscrew the tightening nut remove the wiper assembly and repeat steps 8 10 Wiper Nut n ED a Brush Assembly in O ring Motor Shaft Fastener Mechanical Wiper Specifications 316 Stainless Steel Polypropylene Page 35 Appendix H Installing the Flow Cap Introduction The C6 Multi Sensor Platform s Flow Cap P N 2200 700 was designed to enable flow through mode The Flow Cap will allow the C6 Multi Sensor Plat
23. ers can calibrate Cyclops 7 sensors in either the direct concentration mode raw fluorescence mode or blank subtracted raw fluorescence mode c sam anae O O U U U ETE Channel Calibration Link Label crum 1Rhakeria Grrrl APU 03 230 Stap 1 Press Sat Blank to sat the bank Bonk RAU Yaua to the carent REUL zi Sat ank nid LO Step 2 Enter the value of your standard Standard Value Units and eect the uk ol nen E Sima Pres Set Siaina toant the standard RFU standard value to the curent Sak Standard RPL Stapa Optional Chick the temperature Tame Comeaniation compensation bow to activate E Step I After calibration E complete press Save Calibration eve Callt a Cancel Chick tous uncalibrated Rae Mhucrestente Made 998 2205 Rev K Page 14 ulti Sensor Platform Direct Concentration Mode Values reported in the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted The equation used to calculate concentrations after calibrating to Direct Concentration Mode IS Standard Value units x sample RFU Concentration units Standard RFU Raw Fluorescence Mode Blank Subtracted Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB It is important to note that these values are blank subtracted but are not scaled to a standard they are relative values Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Relative Fluo
24. essories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions 998 2205 Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modification of the instrument is not covered This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for accidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state Damage incurred in shipping is not covered Rev K Page 23 amp ulti Sensor Platform 4 3 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com Carry out any adjustments or tests as suggested by the Technical Support Department If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write t
25. form to be configured with other instruments used in flow through systems The C6 Flow Cap e Eliminates other light sources that may interfere with sensor measurements e Protects sensors from damage during deployment or transport e Helps to limit bio fouling The Flow Cap can also be used as a calibration cup to calibrate Cyclops 7 units using standard calibrating solutions See page 2 for calibration cup use Installation 1 Use a screwdriver to remove the screws that fasten the C6 Multi Sensor Platform s support bracket P N 130 2210 2 Remove the C6 Multi Sensor Platform s support bracket 7 T Support bracket Screws S gt 9 Support bracket Support bracket rods 3 Unscrew the 3 stainless steel rods attached to the C6 endcap 4 Position and mate the base of the Flow Cap to the C6 endcap Flow Cap Base C6 Endcap s 998 2205 Rev K Multi Sensor Platform 5 By hand screw the 3 stainless steel rods onto the C6 endcap until they are fully tightened Support bracket rods O Turning clockwise fully hand tighten the Flow Cap housing onto the Flow Cap base connector NOTE The functions of the 3 stainless steel rods are to hold the Flow Cap Base to the C6 housing and to support the wiper unit If Wiper Unit has been purchased see next page for additional wiper unit and wiper unit bracket installation instructions N Turning clockwis
26. from the standard C6 firmware and has some features that users need to consider before installing Appendix M Integrator Firmware 1 Internal datalogging is NOT possible with this version of firmware The Integrator Firmware is intended for data output only 2 The data output rate defined by this Integrator Firmware is 1 second and cannot be changed to reflect longer or shorter data output rates 3 Once power is applied there is a 15 second delay before data output starts at a 1 second interval 4 Ifthe wiper is enabled it will rotate the set number of rotations upon powering the instrument and every 5 minutes as long as power is continuously supplied 5 Sleep mode is disabled therefore as long as power is continuously supplied the instrument will continue to stream data at a 1 second interval Download Firmware on the Turner Designs website http www turnerdesigns com customer care software a firmware 998 2205 Rev K Page 44 amp ulti Sensor Platform Appendix N Campbell Scientific Datalogger Integration Turner Designs and Campbell Scientific worked together to help our customers easily integrate the C6 Multi Sensor Platform with any of the CR800 or CR1000 series dataloggers Simply follow the steps below to quickly set up your datalogger and begin data logging 1 2 3 4 Download the txt file that pertains to your datalogger series see links below Upload that file to your Campbell Datalogger Run the pr
27. ft holes located on the back of the C6 Multi Sensor Platform s housing using a screwdriver 2 Apply the Loctite threading compound to threads on each screw Loctite and screws are included in bracket package 3 Align the bracket holes with the threaded holes on the back of the C6 Multi Sensor Platform 4 Using the screws included in the battery bracket package fasten the bracket to the back of the C6 Multi Sensor Platform 5 Slide the battery through the bracket towards the male end connector on the C6 Multi Sensor Platform 6 Mate the C6 Multi Sensor Platform 8 pin male connector to the female connector on the battery 7 Ensure that the battery is completely mated to the C6 Multi Sensor Platform 8 Secure the battery using the Allen Hex Key provided to tighten the bracket ring around the battery Page 40 Multi Sensor Platform Appendix K Charging the Submersible Battery Pack Introduction The Submersible Battery Pack P N 2200 601 contains a rechargeable lithium ion battery and battery pack charger The battery pack is used together with the Internal Datalogger to self contained use of the C6 Multi Sensor Platform The battery pack has a 600m depth rating and facilitates vertical profiling and moored applications Charging the Battery 1 Carefully align the battery s connector to the pins of the charger and push the battery straight onto the charger 2 Plug the charger into a standard wall out
28. he C6 Multi Sensor Platform s output is in digital ASCII format 3 6 Downloading and File Management Users can download new and existing files saved in the C6 Multi Sensor Platform Select Download Data Note This step may take several minutes depending on file size 998 2205 Rev K Page 21 Multi Sensor Platform When download has completed choose the files to be saved by clicking the Save box Users will be prompted to choose a folder location where data will be saved Files will be saved in a CSV format C Soft Manager File Settings COMPort Window Help Connected 2 C Soft Manager x Settings Current Data Log Setup Download Data Select files to save Total Files 0 Used Data Log Start Date Time End Date Time Records Save l Coo Peeeepererpereepererpererepeeenrpeereperenrpererpernngy r y r wa 0 10 20 30 40 50 60 70 80 930 100 Start DateTime End Date Time Records Save 0 Start Date Time End Date Time Records Save 0 Start Date Time End Date Time Records Save 0 Start Date Time End Date Time Records Save D Start Date Time End Date Time Records Save 0 Start Date Time End Date Time Records Save 0 Download Data Clear Datalog xl Select Clear Datalog to erase existing data from memory Users are able to upload data and view in the Current Data window if desired Note If you have problems downloading data in C Soft you may have limited sp
29. he RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who purchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 4 4 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2205 Rev K Page 24 amp ulti Sensor Platform 998 2205 Rev K Page 25 Appendix A Specifications C6 Multi Sensor Platform Weight in Air 2 74 kg 6 0 Ibs without sensors submersible battery or wiper Weight in Water 1 6 kg 3 4 Ibs Length 25 8 cm 10 2 in
30. ions the signal will decrease even though the sample concentrations are continuing to increase This effect is known as signal quenching Linearity can be checked by diluting a sample 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range O O O Fluorometer Response Curve a Graph showing Linear and Quenching Regions of the sample s response Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll please refer to the application section of Turner Designs website 998 2205 Rev K Page 34 Multi Sensor Platform Appendix G Installing the Mechanical Wiper Introduction The Mechanical Wiper P N 2200 450 for the C6 Multi Sensor Platform is designed to reduce bio fouling during extended deployments Benefits e Helps maintain consistent readings by reducing bio fouling e Replacement brushes available P N 2200 460 Installation 1 Plac
31. irection until you feel the indexing ball click into the indexed position Cyclops 7 Solid Standard indexing mark Solid Standard indexing ball Align the index mark and indexing ball when mating the Cyclops 7 and the Solid Secondary Standard Rev K Use a flathead screwdriver to unscrew the locking nut as far as it will go To change the signal level use the green screwdriver provided and insert the blade through the hole in the locking nut Rotate the screwdriver until it engages with the adjustment screw that is beneath the locking nut Rotate the screw to adjust the signal level as necessary Turning clockwise increases the signal and counterclockwise decreases the signal Lock nut Adjustment screw is located under the locking nut Insert the supplied green screwdriver through the hole in the locking nut to reach the adjustment screw Once the desired reading is obtained tighten the locking nut so the adjustment screw is held firmly In place Finish by noting the output voltage and gain setting used x1 x10 or x100 in the Value space on the Secondary Standard label Note that the response of every Solid Secondary Standard is unique Hence a new correlation must be determined for every sensor For future identification use the ID space on the label for a unique identifier for the Secondary Standard Page 32 Use of the Solid Secondary Standard for in vivo Chlorophyll Applications 1
32. let and allow the battery to charge for 6 hours for a complete battery charge 3 When finished unplug the charger and remove the battery by pulling the battery straight out from the charger s connector Battery s Connector Charger pin housing General Precautions Under no circumstances should you attempt to open the battery housing Please contact Turner Designs if you have any problems or questions related to the battery pack 998 2205 Rev K Battery Life The battery is designed when fully charged to exceed the Internal Data Logging capacity at the maximum sampling rate For longer deployments at shorter sampling intervals we recommend recharging the battery after the Internal Datalogger is full or every 3 4 months whichever comes first Note Use the Battery Life Calculator available on the Turner Designs website C3 C6 product pages to calculate a conservative estimation of how many days you can expect to log data Factors such as battery charge and water temperature will affect logging days Specifications Dimensions eS 13 46cm x 7 0cm Weight in Air 1 65lbs 0 75kg Weight in Water 0 50Ibs 0 22kg Rated Depth 600m 1969 feet Voltage Output 14 8V nominal Capacity 2 200MAH 8 pin connector Charge Time Connector Protects against Overcharge Over Discharge Overheating Short Circuiting Protection Features Charge with Turner Designs charger only Page 41 a
33. mp ulti Sensor Platform Appendix L How to set up Hyperterminal for Digital ASCII Data Capture 1 Ensure C Soft program is not running in the background On an MS Windows computer open the HyperTerminal program Start Programs Accessories Communications gt HyperTerminal The Connection Description screen will appear Name the connection description This file will save the communication parameters for the C6 Multi Sensor Platform and can be used in the future to establish communication with the instrument quickly The Connect To screen will appear Choose the appropriate communications port in the Connect Using window Click OK The Port Settings screen will appear Choose the following port settings Bits per second 9600 2i xl Data bits 8 Port Settings Parity None Stop bits 1 Bits per second ESI Flow control Hardware ae i ata bits Click Apply and OK Parity None Stop bits fi Flow control Hardware v Restore Defaults Cancel Apply 5 Next the HyperTerminal window will appear You are now ready to connect the C6 Multi Sensor Platform to a power source Data will be displayed at set intervals in the HyperTerminal window after 100 seconds or at the specified log start time C Soft HyperTerminal i loj x File Edit View Call Transfer Help Date Time RhodaminePhycocyani Depth Temp C 6 16 08 15 07 24 98 65 129 70 0 00 23 71 6 16 08 15 07 25 51 09 130 71 0 00 23
34. n Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial seawater or filtered seawater Note Ensure the Cyclops 7 sensors are free of bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Selecting Units Select RFUB from the units dropdown menu Note Do not enter a value in the standard value box Step 3 Skip and go to Step 4 Step 4 Optional Temperature Compensation Follow same steps under the Direct Calibration procedure in section 3 3 1 Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as blank corrected relative fluorescence units Rev K Page 17 amp ulti Sensor Platform 3 3 3 Raw Fluorescence Calibration In the Current Data screen select the calibrate button next to a specific channel At the bottom of the Calibration Screen select Click to use uncalibrated Raw Fluorescence Mode Data for that channel will be uncalibrated and reported as Relative Fluorescence Units RFU 3 3 4 Depth Calibration Note Make sure your instrument is calibrated for depth and there is a value set even if you do not have a depth sensor Depth is displayed in meters only There are two calibration options for depth calibration 1
35. nsor Platform 998 2205 Rev K Page 7 G Platform Cyclops 7 Fastener 998 2205 Rev K Page 8 ulti Sensor Platform 3 C Soft Windows User Interface The C Soft Windows based user interface allows for intuitive calibration data logging set up and download functions 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 4 4 133MHz microprocessor 600 X 800 VGA resolution Microsoft Windows 98 32 MB RAM 3 1 2 Software Installation 1 2 Exit all Windows programs Insert the C Soft CD also located on the Turner Designs Software Firmware webpage under Customer Care Open the C Soft software folder and double click on the setup icon The software will automatically be installed in the PC s C Program Files C6 folder unless user selects alternate location After restarting the C Soft icon is located on the desktop Cote Soft Note You may receive a warning message asking if you want to unblock messages select Unblock 3 1 3 Integration Interface Adapter Cable Installation 1 998 2205 Insert the C6 software CD and install the Adapter Cable driver Integration Interface_Adapter__Drivers exe onto your PC Make sure nothing is plugged into the Adapter Cable s connectors Plug the USB connector into a USB port on your computer and wait for hardware to automatically install Once USB is installed and ready to use right cli
36. ogram using your Campbell Datalogger Enable the Data Output Ready Mode on your C6 this will allow streaming of ASCII data at the set sample interval Plug your C6 into the datalogger and watch data stream or capture data using your datalogger Download Firmware on the Turner Designs website http www turnerdesigns com customer care software a firmware NOTE Be sure to use the Integration Cable or Integration Interface Adapter Cable to plug the C6 Multi Sensor Platform into your datalogger using the Standard Interface Cable may result in the loss of data or inability to communicate with your datalogger Be sure to refer to the power requirements in Appendix A under or over power to the instrument may result in damage and loss of data 998 2205 Rev K Page 45 Ge PN Application MDL Dynamic Range Appendix O Optical Specification Guide LED CWL Excitation Emission Sol Std XXXX 000 O 0 2 ppb 0 2700 ppb 2100 904 905 XXXX 000 G Oil Fine ae a ee 285nm lt 290 nm 350 55 nm 2100 904 905 2 ppm gt 100 ppm Soan Ceeet eaae ter 0 6ppb 0 15 000 ppb 365nm 325 120nm_ 445 15nm 2100 904 905 Wastewater Treatment Xxxx 000 P Phycocyanin Freshwater 5 Pc 0 40 000 ppb 590nm lt 595nm 2630nm _ 2100 900 908 Cyanobacteria eee Varna 1 5 Naphthalene Disulfonic Disodium Salt Quinine Sulfate PTSA 1 3 6 8 Pyrenetetrasulfonic Acid Tetrasodium Salt BTEX Benzene Tol
37. ptional and depends on the sampling interval see section 3 4 5 Click the Enable Datalog button followed by the Yes button to enable the Data Output Ready State Note Use the Battery Life Calculator available on the Turner Designs website C6 product page to calculate a conservative estimation of how many days you can expect to log data Factors such as battery charge and water temperature will affect logging days A few seconds after the Yes button has been clicked to enable the Data Output Ready State the C Soft program will automatically shut down and users must disconnect power and serial connections The instrument is now in the Data Output Ready State and will remain in that state so long as C Soft is not used to communicate with the instrument Note As soon as C Soft is used to communicate with the instrument users will need to re set the parameter settings in the Log Setup section 3 4 and again enable datalog to put the instrument into the Data Output Ready State When power is supplied to the instrument while it is in the Data Output Ready State it will begin streaming data after a 100 second delay If power is cycled i e disconnected and reconnected please allow 100 seconds for the data stream to begin Note Check the optical face when power is supplied to make sure the sensors light The sensor will turn on then off and then turn back on in 100 seconds If this sequence does not happen cycle power T
38. rd to produce the same response in the same gain as in step 1 3 The Solid Secondary Standard s signal is now equivalent to the concentration value of the dye solution used in step 1and can be used in place of a liquid primary standard for future calibration of that specific Cyclops 7 Note Comprehensive information on dye trace measurements can be found at the following Turner Designs website http www turnerdesigns com fluoresce nt dye tracing Care and Storage The Solid Secondary Standard should be stored at room temperature 20 degree C in the case when not in use and kept free of dust and moisture Special care must be taken with the UV Solid Secondary Standard P N 2100 904 to ensure that it is not exposed to UV light for prolonged periods of time This can result in degradation of the standard Page 33 Platform Appendix F Linear Range Quenching and Temperature Considerations The linear range is the concentration range in which the Cyclops 7 output is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrat
39. re Considerations G Installing the Mechanical Wiper H Installing the Flow Cap Installing the Shade Cap J Installing the Battery Bracket and Battery K Charging the Submersible Battery Pack L How to Set up Hyperterminal for Digital ASCII Data Capture M Integrator Firmware N Campbell Scientific Datalogger Integration O Optical Specification Guide WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT WEEE DIRECTIVE Turner Designs is in the business of designing and selling products that benefit the well being of our environment Accordingly we are concerned with preserving the surroundings wherever our instruments are used and happy to work with customers by complying with the WEEE Directive to reduce the environmental impact resulting from the use of our products WEEE Return Process To arrange the return of an end of life product proceed as follows If you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end of life product If you purchased your instrument directly from Turner Designs please contact Turner Designs Customer Service By Phone 1 408 212 4041 or Toll Free 877 316 8049 By Email Customer Service at support turnerdesigns com Turner Designs will provide a WEEE RMA Number a Shipping Account Number and a Ship to Address Package and ship the product back to Turner Designs The product will be dealt with per Turner Designs
40. rescence Units RFU It is important to note that these values are not blank subtracted or scaled to a standard they are relative values 3 3 Calibration Procedures 3 3 1 998 2205 Direct Concentration Calibration Following steps 1 5 will ensure values are reported as Blank Subtracted Concentrations in units of choice See Appendix B for recommended practices Step 1 Blanking Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a blank solution Note A blank solution is a solution without the fluorophore of interest i e deionized water artificial seawater or filtered seawater Note Ensure the Cyclops 7 sensors are free of air bubbles Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Rev K Page 15 ulti Sensor Platform Step 2 Standard Value and Units Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a standard solution for the specific sensor being calibrated or use a solid secondary standard See Appendix E for more information on solid secondary standards Note Turbidity sensors do not have solid secondary standards Enter the known concentration value of the standard solution or solid standard in the Standard Value box If the concentration is not known enter an arbitrary value Enter manually or select desired unit
41. s from the dropdown menu Note Do not choose or enter RFUB if Direct Concentration Mode is used Step 3 Set Standard When readings in Current RFU box have stabilized select the Set Standard button Step 4 Optional Temperature Compensation Temperature compensation is optional it enables the C6 to compensate for changes in fluorescence due to varying temperatures as detected by the C6 s temperature sensor Temperature compensation is available for in vivo Chlorophyll and Rhodamine sensors only Temperature is displayed in degree Celsius Click on the Temperature Compensation box Select either in vivo Chlorophyll or Rhodamine from the dropdown menu Data will now be corrected for temperature fluctuations The temperature compensation coefficients for each fluorophore are listed below Fluorophore Coefficient 0 026 C Exponential in vivo Chlorophyll 1 4 C Linear 998 2205 Rev K Page 16 3 3 2 998 2205 sensor Platform Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as concentration estimates Raw Fluorescence Calibration Blank Subtracted Following steps 1 5 will ensure values are reported as Blank subtracted Relative Fluorescence Units RFUB See Appendix B for recommended practices Step 1 Blanking Place the C6 Multi Sensor Platform and Cyclops 7 sensors in a blank solutio
42. s not in use if all are in use then choose any port 1 6 Click OK to the warning box if it appears and Click OK to close any other open menus Your installation is complete Make sure to set C Soft to that specified COM designation when trying to connect via USB Adapter Cable connection PC Software Interface Note It is important that the steps be followed INORDER Any variance from the procedure may result in the connection NOT being established 1 Plug either the USB or serial connector of the adapter cable into your computer Note Either connection can be used for communicating and configuring the instrument Plug the interface cable onto the 8 pin bulkhead connector of the instrument Plug the interface cable s serial plug into the adapter cable s male serial plug Begin C Soft by double clicking on the C Soft icon and choose the appropriate COM port from the pull down COM menu Plug the 12V power supply into a power source Wait for initialization process to complete Rev K Page 10 amp ulti Sensor Platform 7 The connection icon located on the upper right of the screen will turn green if connected C Soft Manager File Settings COM Pork Window Help Connected Note If the connection icon does not turn green disconnect and reconnect power 3 2 Software Operation 3 2 1 Settings Screen The Settings Screen allows users to set the time and date identify each Cyclops 7 upda
43. te serial numbers and select sensors to be enabled Users can manually set the date and time or choose to synchronize the C6 Multi Sensor Platform to the PC date and time Each channel 1 6 in the Settings Screen has a port associated with it on the C6 Multi Sensor Platform that is numerically labeled 1 6 respectively Click on the box adjacent to the channel to enable channel operation If there is no sensor installed users will be unable to select that channel Channels can be renamed using the dropdown selections or by manually entering a name Channels can also be named using Cyclops 7 serial numbers Settings will automatically be saved after exiting the Settings screen 998 2205 Rev K Page 11 Multi Sensor Platform File Settings COM Port Window Help Settings Current Data Log Setup Download Data C Soft Manager Channel Settings Enable Mame Serial Chani M Rhodamine Unit Current Time rhan F 10 32 20 j it Ti Chan 2 Chlorophyll a RE Retrieve Unik Time Chan 3 v Phycocyanin Chan 4 Current Time i i 10 32 27 Set Unit To PC Date amp Time Chan 5 6 16 2008 Chan 6 Depth fv Manually Set Unit Dake amp Time Temp Set Unit Clack 3 2 2 Current Data and Calibration Screen The Current Data Screen allows users to view real time data acquisition in both tabular and waveform formats as well as conduct calibrations for each channel and view calibration information
44. the sensor is more than 3 inches above the bottom of the container 4 Ensure that the sensor is in the center of the container and has more than 2 inches clearance between the sensor and the inside surface of the container 5 If using a glass beaker check if the optical surface of the sensor is free of air bubbles Remove air bubbles if any are observed If using a dark plastic container run the wiper or your finger over the optical sensors to ensure the sensor heads are free of air bubbles TW ANER s f Calibrated Sensor gt 2 inches all round Glass Beaker gt inches No Air Bubbles On Optical Surface Dark Black Non Reflective Surface 998 2205 Rev K Page 27 Multi Sensor Platform Appendix C Cable Guide Computer Interface Cable P N 2200 150 This cable is included as a standard accessory with the C6 It is used to program and communicate with the C6 using C Soft and provide real time data view This cable is NOT recommended for serial data output The cable length is approximately 2 5 feet Integration Interface Adapter Cable P N 2300 115 This cable is included as a standard accessory included with the C6 This cable works with the Computer Interface Cable P N 2200 150 It allows users to upload firmware to the instrument using the USB or stream digital data continuously using the RS232 plug Either plug can be used to communicate and configure the instrument
45. uene Ethylbenzene Xylenes a Phycocyanin pigment from Prozyme diluted in Deionized water htip prozyme com PE Phycoerythrin pigment from Prozyme diluted in Deionized water http prozyme com 998 2205 Rev K Page 46

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