User`s Manual
Contents
1. Petri Dish Electrode USER S MANUAL HARVARD APPARATUS The Electroporation Experts INTRODUCTION The Petri Dish Electrode is designed to be used with a 100 mm Petri dish which functions as the electroporation chamber To perform electroporation or electrofusion the samples are placed in the Petri dish and the electrode assembly is lowered into the dish The electrodes are 2 mm apart and are made of stainless steel They are parallel and thus create a homogeneous field They are designed to electroporate volumes ranging from 10 to 50 ml The 2mm gap Petri Dish Electrode can be used for electroporation of tissues grown right in the petri dish Simply add the exogenous molecule of interest mix and electroporate When electroporating adherent cells the distance between the electrodes and the petri dish can be adjusted by placing plastic foil underneath the petri dish The electrodes need to be in contact with the cells but not cutting through IMPORTANT Read all Instructions Warnings and Precautions prior to use FOR RESEARCH PURPOSES ONLY Order No Model Description 45 0100 366 Petri Dish Electrode 2 mm Gap Stainless Steel tollfree 800 272 2775 ioca 508 893 8999 www btxonline com Petri Dish Electrode GENERAL INFORMATION Warranty BTX Harvard Apparatus warranties this BTX Petri Dish Electrode for a period of 90 days from date of purchase At its option BTX Harvard Apparatus will repair or replace2. 800 272 2775 s local 508 893 GENERAL SAFETY INFORMATION Review the following safety precautions to avoid injury and prevent damage to this product or any products connected to it To avoid potential hazard use this product only as specified Only qualified BTX personnel should perform service procedures To Prevent Hazard or Injury ARCING CAN OCCUR AT HIGH VOLTAGES An unfavorable combination of parameters such as high voltage settings and a small sample volume with a highly conductive medium might lead to flashover between the electrodes ARC and or explosive evaporation of the medium Reduce voltage or pulse length to avoid repeating this condition DO NOT OPERATE WITH SUSPECTED FAILURES If you suspect there is damage to the product have it inspected by qualified BTX service personnel DO NOT CONTACT ELECTRODES To avoid fire or shock hazard observe all ratings and markings on the product or in this manual before using the device AVOID EXPOSURE TO CONTACT Do not insert fingers or try to remove electrode or sample during pulsing sequence WEAR PROPER EYE PROTECTION DURING ELECTROPORATION DO NOT OPERATE IN AN EXPLOSIVE ENVIRONMENT DO NOT OPERATE IN WET DAMP CONDITIONS Safety Terms and Symbols Terms that appear in this manual WARNING Warning statements identify conditions or practices that could result in injury or loss of life CAUTION Caution statements identify A conditions or practices that could result in Symb
3. Gap Stainless Steel 45 0059 VIP3000SC_ The Enhancer 3000 Monitoring System APPENDIX C TROUBLESHOOTING Please contact BTX Technical Service at any of the numbers listed below in the event of any failure BTX A Division of Harvard Apparatus 84 October Hill Road Holliston MA 01746 1388 U S A Toll Free 1 800 272 2775 US only Phone 1 508 893 8999 Fax 1 508 429 5732 E mail techsupport btx harvardapparatus com Internet www btxonline com click on customer service APPENDIX D MAINTENANCE A WARNING Do not attempt maintenance while the Petri Dish Electrode is plugged into a pulse generator Clean the Petri Dish Electrode body with a soft cloth or tissue If necessary moisten the cloth or tissue with a dilute detergent solution Clean the electrodes between each experiment by washing with a mild detergent using a cloth or soft bristled brush tollfreee 800 272 2775 loca 508 893 8999 www btxonline com WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directi
4. ols that may appear on the products damage to these products or other property A A Danger Attention Protective Functional High Refer to Earth Ground Voltage Manual Terminal Terminal 8999 www btxonline com Petri Dish Electrode OPERATION GETTING STARTED A WARNING HIGH VOLTAGE Make sure the BTX electroporator is switched off before continuing 1 Attach the banana cable into the voltage output of the BTX electroporator If using the Enhancer 3000 Monitoring System attach the cables to the Enhancer 3000 and then attach the cables from the Enhancer 3000 to the electroporator Refer to the Enhancer 3000 for instructions for use 2 Following instructions for the BTX electroporator set the appropriate parameters 3 Prepare tissue and sample for electroporation 4 Deliver the electroporation pulse s to the sample 5 Remove the electrodes carefully and prepare for the next experiment 6 The Petri Dish Electrode is easy to clean Wash with soap and water and sterilize with alcohol APPENDIX A SPECIFICATIONS Petri Dish Electrode Electrical amp Technical Specifications Standard Capabilities Voltage Range 0 to 2000 VDC Do not use AC current Pulse Length Range 1 psec to 40 msec Pulse Number Range 1 to 99 depending on voltage Operating Temperature 5 to 40 C Intended Use Relative Humidity Indoor use only 20 to 80 2 000 m 6 562 ft Pollution Degree II Maximum Altitude Insulati
5. on Category CAT Physical Characteristics Dimensions Lx Wx D 15 2 x 10 2 x 6 4 cm 6 x 4 x 2 5 in Volume Range 10 to 50 ml Electrode Gap 2mm Electrode Material Stainless Steel Compatibility Generators ECM 830 630 and 2001 Monitoring The Enhancer 3000 Monitoring System recommended CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS Petri Dish Electrode MA1 45 0100 Max volume and voltage settings Table A illustrates the maximum volume and the voltage settings that can be used in order to obtain the maximum pulse length in a 2mm gap Petri Dish Electrodes In the HV mode the maximum pulse length using a non conductive media H20 at 2000 volts and 50 ml volume is 4 3 msec Warning Do not set the voltage higher than 2000 volts since an arc may occur If the volume is decreased to 15 ml the maximum pulse length is 7 3 msec using a non conductive media H20 Table A Experiment 1 2 3 4 5 6 7 Media HO H O H O PBS PBS PBS PBS Volume ml 50 50 15 10 10 10 10 Voltage Mode HV HV HV LWW LW UON W Set Voltage V 500 2000 2000 100 150 250 500 Actual Voltage V 480 2000 2000 60 101 195 445 Set Capacitance uF 50 50 50 3175 3175 3175 3175 Set Resistance Ohms R10 R10 R10 R10 R10 R10 R10 Pulse Length msec 5 3 43 7 3 1 9 1 67 1 3 1 26 Chamber Resistance Ohms 160 153 400 0 4 0 37 0 37 0 38 APPENDIX B REPLACEMENT PARTS Order No Model 45 0100 366 Description Petri Dish Electrode 2 mm
6. rom state to state Note BTX electrodes are not recommended for use with power supplies or cables from other manufacturers Such use is completely at the customer s own risk as it may result in damage create unsafe conditions and will immediately void the 90 day warranty IMPORTANT Read all Instructions Warnings and Precautions prior to use Technical amp Customer Service BTX is the ultimate resource for technical information on the use of high voltage bacterial transformation and general electroporation of molecules and drugs into cells We constantly track and monitor scientific publications in this area Our Technical Service group extracts and enters pertinent information such as results and parameters from these papers into a Protocol database This database is available via the BTX website Please visit www btxonline com For technical assistance additional information or an inquiry request for repair service contact BTX Harvard Apparatus Technical Support Customer Service Group at BTX A Division of Harvard Apparatus 84 October Hill Road Holliston MA 01746 1388 U S A Toll Free 1 800 272 2775 U S only Phone 1 508 893 8999 Fax 1 508 429 5732 E mail techsupport btx harvardapparatus com Internet www btxonline com click on customer service If outside the United States and Canada call 1 508 893 8999 or contact your nearest BTX Distributor A complete list of distributors is on our website toll free
7. the item if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchase IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the 90 day warranty period promptly contact BTX Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 2775 Outside the U S call 1 508 893 8999 Goods will not be accepted for return unless an RMA Return Materials Authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs or replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary f
8. ves will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products Do Not Dispose Product with Municipal Waste and Category 9 monitoring and control instruments Most of our e Special Collection Disposal Required products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any changes as they apply HARVARD APPARATUS The Electroporation Experts 84 October Hill Road e e Holliston MA 01746 toll free 800 272 2775 local 508 893 8999 fax 508 429 5732 emai Te chsupport btx harvardapparatus com gt weowww btxonline com Publication 5502 005 REV CS
Download Pdf Manuals
Related Search