PIPETMAN® - Pipette Supplies
Contents
1. 5 VOLUME INDICATOR LCD WINDOW What you normally see D Volume setting Units mL or uL 3 Volume status locked or unlocked What could appear 4 Calibration status RECAL this means that your pipette has been readjusted 5 Battery status failing LOW BATT here or failed BATT in place of volume setting ESI PIPETMAN o E Cycle Counters You will see the counters at the right hand side of an otherwise blank screen a Since the last volume setting holding down the push button for 7 seconds displays this in formation maximum 199 ag for example 126 cycles b Since the pipette was made holding down the push button for 10 seconds displays this information maxi mum 999999 for example 285358 285396 cycles gt Refer to Troubleshooting for possible error messages 6 SETTING THE VOLUME You must perform the following steps 1 Switch on press the push button to the first stop LCD shows the volume currently set 2 Unlock push the thumbwheel upwards LCD shows UNLOCKED also visible on the thumb wheel 3 Set the volume rotate the thumbwheel or the push button clockwise to decrease the volume counterclockwise to increase when decreasing the volume reach the required setting slowly making sure not to overshoot the mark when increasing the volume pass the required value by 1 3 of aturn then decrease the volume slo2. distilled water Internal The following components only can be immersed in a cleaning solution tip ejector tip holder both parts connecting nut piston including holder return spring and spring guide 1 Disassemble the pipette as described in Main tenance 2 Set aside the upper part in a dry and secure location 3 Clean the individual components of the lower part of the pipette using an ultrasonic bath 22 20 minutes at 50 C or with a soft cloth and brushes Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder 4 Rinse the individual components with distilled water 5 Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue 6 Lubricate the piston and reassemble the pipette according to the instructions given earlier in this chapter Decontamination Autoclaving The body handle of the pipette is not autoclav able Only the following parts may be autoclaved tip ejector tip holder both parts connecting nut piston including holder return spring and spring guide The O ring is not autoclavable it should be replaced with the one specified in Spare Parts 1 Clean the parts to be autoclaved especially the tip holder 2 Put the parts in an autoclaving sack 3 Autoclave for20 minutes at 121 C and0 1 MPa 4 Check that the parts are dry before re assembling the pipette 5 Set the
3. film of lubricant is required over the entire piston Changing the Battery The battery is a silver oxide plated 1 5V generic code 386 size 11 6 x 4 2 mm gt 1 Remember that locking your pipette when not in use will maximize the life of the battery 2 Although changing the battery does not affect the calibration you may wish to take this opportunity to perform a user adjustment recalibration of the pipette If you need to change it proceed as follows 1 Ensure the pipette is in the locked position remove the tip ejector 2 Remove the battery compartment cover Tool the calibration key is equipped with a tool for opening the battery compartment cover Then perform the following steps with refer ence to the photographs maintain the tip ejector holder in the fully extended position by holding down the tip ejector button insert the tool side marked UP facing you into the space between the cover and the tip ejector holder 20 push firmly in the direction shown by the arrow then gently lever the cover upwards from the pipette 3 Pull outthe battery holder red plastic Battery negative terminal 4 Liftupandremovethecop per clip refer to Figure 5 Lever out the old battery and put it in a secure bin for recycling Copper clip lift here Cover Battery Compartment gt k gt 6 Insert a new battery see Spare Parts int
4. lt 6 60 lt 30 F21028 5mL 40 lt 10 60 lt 30 Max 10mL 60 lt 16 60 lt 30 3 The data given in the table conform to the ISO 8655 2 Standard 27 EI PIPETMAN o E Pipetman Ultra is a high quality pipette that offers excellent accuracy and precision The figures given in the Gilson Maximum Permissible Errors table were obtained using Gilson Diamond Tips These figures are only guaranteed using Genuine Gilson Diamond Tips Each pipette is inspected and validated by quali fied technicians according to the Gilson Quality System Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 Standard by type testing The adjustement is carried out under strictly defined and monitored conditions ISO 8655 6 Basis of adjustment Ex Reference temperature 20 C Relative humidity 50 Barometric pressure 101 kPa Use of distilled water grade 3 ISO 3696 Ten measurements for each test volume which are Nominal Volume 50 of Nominal Volume and the minimum or 10 of Nominal Volume 28 17 SPARE PARTS U2 F21021 and U10 F21022 mooo uv gt h ZEZFIILZoNI hk Description Push button Spring guide Connecting nut Seal x 5 Piston assembly Return spring Tip holder upper Tip holder lower Tip ejector Battery Holder Battery Recalibration Tool Cover battery compartment Plastic Window Tip ejector extension U2 F207015
5. of the collar is different Also tip ejector extensions are supplied for U2 and U10 pipettes 12 Tip ejector Extension for Use with Ultra U2 and U10 In order to eject D10 tips tip ejector extensions are supplied with U2 and U10 pipettes ges The tip ejector extension which is made of PVDF polyvinylidene Fluoride is autoclavable To fit a tip ejector extension hold the pipette with the LCD window upper most hold the extension with the slot uppermost slide the extension over the tip holder push the extension firmly onto the end of the tip ejector until it clicks into place See opposite To remove a tip ejector extension see opposite hold the pipette in one hand and grip the extension with the other gently twist the extension either direction and pull it away from the pipette Personal Label Name Tag You ean identify Place Name Tag Here your pipette with a name tag Pe N 1 Pry out the window by A Access Slot i s driver H inserting a 7 7 Tsen crewdriver Here Plastic Window small screw driver in the access slot 2 Position the name tag next to the LCD 3 Clip the window back into place ESI PIPETMAN o E 10 GLP FEATURES These are as follows e Locked volume e Serial Number is engraved on the body of the pipette and encoded in the bar code e Bar Code on the box and with the certificate can be transferred e Name Tag Application
6. rod gi 3 Pull the tip ejector away from the body of the pipette 4 Clean or autoclave the tip ejector and refit it or a new one by reversing the procedure Changing the Tip holder lower part After removing the tip ejector you may remove 16 the lower part of the tip holder which is more likely to become contaminated or damaged than the upper part Removal of the lower part is shown below for the upper part see Removing the Piston special precautions are necessary 1 Gently rotate the lower part Tip holder upper of the tip holder y amp counterclock wise to unscrew itfrom the upper part Tip holder lower 2 Separate the parts and remove the O ring see Changing the O ring 3 Clean and if required autoclave the lower part of the tip holder 4 Ifrequired lubri cate the piston see How to Lubricate the Piston and fit a new O ring 5 Screw the two parts together making sure that the two parts are fully tightened by hand 6 Refit the tip ejector Changing the O ring or seal for U2 and U10 The O ring is contained by the two halves of the tip holder it must not be autoclaved if worn or damaged in any way it must be replaced To access the O ring remove the tip ejector and unscrew the lower part of the tip holder if the O ring is not immediately visible on the piston set the pipette to its maximum volume then press the push button t
7. 07 F2072107 N Plastic Window F2072102 F2072102 ES For U1000 the piston assembly is an assembly part composed of spring guide return spring and piston assembly 31 ESI PIPETMAN o E U5000 F21027 and U10ml F21028 Zg agrgzonmor h 32 Description Push button O ring x 5 Piston assembly Tip holder Tip ejector Battery Holder Battery Recalibration Tool Lubricant Cover battery compartment Plastic Window U5000 F2070752 F2070701 F2070760 F2070718 F2070759 F2070158 F20710 F20751 F2070902 F2072107 F2072102 U10ml F2070852 F2070801 F2070860 F2070818 F2070859 F2070158 F20710 F20751 F2070902 F2072107 F2072102 NOTES 33 ESI PIPETMAN o B NOTES OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOODOOOOOOOOOOOOOOOOOOOOOOOOO EC DECLARATION OF CONFORMITY iu GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 www gilson com Hereby certifies on its sole responsibility that the products listed below Pipetman Ultra U2 U10 U20 U100 U200 U1000 U5000 U10ml 98 79 EC comply with the requirements on In Vitro Diagnostic of the following European Medical Devices Directives 89 336 EEC Electromagnetic Compatibility EMC Annex III self declared Villiers le Bel October 5th 2004 S Solotareff R Pascal General Manager Quality Director D000000000000000000000O0O0O0
8. 10 only Certificate of conformity including bar code sticker ESI PIPETMAN o E 3 DESCRIPTION Legend to figures D Push button for switching on setting the volume aspirating and dispensing Button is color coded with the volume range engraved on the top 2 Ergonomic stabilizer to make the pipette more comfortable to use and to reduce fatigue 3 Thumbwheel to set and lock the volume 4 Tip ejector button can be positioned for left or right handed operation 5 aLiquid Crystal Display LCD window for volume setting and status indicators S bldentity tag window see GLP features 6 Connecting nut for upper part contains piston of removable tip holder D Tip ejector stroke adjustment wheel Tip ejector 9 Tip holder removable for cleaning and or autoclaving Table Operating Ranges Model Range Color Code Reference U2 0 2 2 uL Orange F21021 U10 1 10uL Red F21022 U20 2 20 UL Clear yellow F21023 U100 20 100 uL Peach F21024 U200 20 200 uL Yellow F21025 U1000 200 1000uL Blue F21026 U5000 1 4 mL Purple F21027 U10ml 1 10mL Clear blue F21028 4 SWITCHING ON Press the push button to the first stop to switch on the pipette The information displayed in the LCD window depends on the status of the pipette and the volume currently set See below gt Pipetman Ultra turns itself off after 3 min of inactivity just press the push button to reactivate the pipette
9. 2 F20701031 F2072111 F161901 F2070160 F2070125 F2070117 F2070118 F2070159 F2070158 F20710 F20751 F2072107 F2072102 F2070903 U10 F2070252 F20701031 F2072111 F161902 F2070260 F2070125 F2070117 F2070218 F2070159 F2070158 F20710 F20751 F2072107 F2072102 F2070903 29 EI PIPETMAN o E U20 F21023 and U100 F21024 30 Description Push button Spring guide Connecting nut O ring x 5 Piston assembly Return spring Tip holder upper Tip holder lower Tip ejector Battery Holder Battery Recalibration Tool Lubricant Cover battery compartment Plastic Window U20 F2070352 F20701031 F2072111 F161903 F2070360 F2070125 F2070117 F2070318 F2070359 F2070158 F20710 F20751 F2070902 F2072107 F2072102 U100 F2070452 F20701031 F2072111 F2070401 F2070460 F2070125 F2070417 F2070418 F2070459 F2070158 F20710 F20751 F2070902 F2072107 F2072102 U200 F21025 and U1000 F21026 Description U200 U1000 A Push button F2070552 F2070652 B Spring guide F20701031 j C Connecting nut F2072111 F2072111 D O ring x 5 F2070501 F2070601 E1 Piston assembly F2070560 F2070664 E2 Return spring F2070125 j Fi Tip holder upper F2070517 F2070617 F2 Tip holder lower F2070518 F2070618 G __ Tip ejector F2070559 F2070659 H1 Battery Holder F2070158 F2070158 H2 Battery F20710 F20710 K Recalibration Tool F20751 F20751 L Lubricant F2070902 F2070902 M Cover battery compartment F20721
10. 200 fit a Gilson Diamond Tip set the pipette to the nominal volume given in the specifications pre rinse the tip then aspirate the set volume from a beaker of distilled water maintain the pipette in the vertical position and wait for 20 seconds 24 if a water droplet appears at the end of the tip there is a leak see Troubleshooting if you see no droplet re immerse the tip below the surface of water the water level inside the tip should remain constant if the level goes down there is a leak see Troubleshooting U1000 to U10 ml fit a Gilson Diamond Tip set the pipette to the nominal volume given in the specifications aspirate the set volume from a beaker of water maintain the pipette in the vertical position and wait for 20 seconds if a water droplet appears at the end of the tip there is a leak see Troubleshooting 15 USER ADJUSTMENT The following procedure is primarily used to check the accuracy of a pipette following servicing for example after replacing the piston assembly Recalibration must be carried out by trained people under the correct conditions and using the equipment described in Verification Procedure for Accuracy and Precision For more details you can download the procedure on the Gilson website www gilson com or contact your Gilson distributor who can help you to implement these pipette checking procedures or to build up y
11. EI PIPETMAN v E Other languages available on our website www gilson com ESI PIPETMAN o E CONTENTS page 1 INTRODUCTION 2 2 PARTS CHECK LIST 3 3 DESCRIPTION 4 4 SWITCHING ON 5 5 VOLUME INDICATOR LCD WINDOW 5 6 SETTING THE VOLUME 6 7 GILSON DIAMOND TIPS f 8 PIPETTING 9 9 PERSONALIZING YOUR PIPETTE 12 10 GLP FEATURES 14 11 TROUBLESHOOTING 14 12 MAINTENANCE 16 13 CLEANING AND DECONTAMINATION 21 14 LEAK TEST 24 15 USER ADJUSTMENT 25 16 SPECIFICATIONS ZT 17 SPARE PARTS 29 Notes 33 EC DECLARATION OF CONFORMITY 35 1 INTRODUCTION Congratulations on acquiring your new Pipetman Ultra Enjoy the following features Ease of use continuously adjustable parallax free electronic display for easy volume setting flashing indicators if volume set is out of range ergonomic design maximum comfort for left or right handed users light balanced low ejection force simple to maintain and adjust other GLP features Performance accurate and precise unbeatable results with Gilson Diamond tips Applications for any type of laboratory suitable for most liquids 2 PARTS CHECK LIST Just take a moment to verify that the following items are present Pipetman Ultra User s Guide Sample pack of tips Safety bag Calibration tool Tube of lubricant except for U2 and U10 Name tags 5 different colors Tip ejector extension U2 and U
12. O0O0O0O0O0O0OO0O00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000O0O00O0O0O0O00000OOOOON CE rasg VOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO OPOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOQ 35 LT801441 F 92005 Gilson SAS All right reserved March 2005 English Printed in France SOLUTIONS AT WORK FOR YC World Headquarters Gilson Inc 3000 W Beltline Hwy P O Box 620027 Middleton WI 53562 0027 USA Telephone 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Gilson SAS 19 avenue des Entrepreneurs BP 145 95400 Villiers le Bel France Telephone 33 1 34 29 50 00 Fax 33 1 34 29 50 20
13. Spare Parts 5 Clean and autoclave if required the piston and holder together with any other parts that may need to be treated in the same way see Cleaning and Decontamination 6 Lubricate the piston see next page N Reassemble the piston spring and spring guide then carefully insert the assembly into the body of the pipette The spring guide should hold the piston assembly inside the body of the pipette Take care to position the small diameter end of the spring as shown innermost and that the flanged end of the spring guide is outermost Spring small diameter end Spring guide flanged M l 8 Reassemble the upper part of the tip holder and the connecting nut then refit to the body of the pipette by rotating the connecting nut clockwise until it is finger tight Fitthe O ring or for U2 and U10 the seal and reassemble the lower part of the tip holder Refit the tip ejector Connecting Nut i 3 The seals for U2 and U10 are fragile and can only be used once So after unscrewing the lower part of the tip holder you must fit a new seal EI PIPETMAN o E Howto Lubricate the Piston except U2 and U10 Use only Gilson lubricant ref F2070902 as supplied Squeeze a small quantity from the tube onto a clean nonabrasive cloth Use the cloth to transfer the lubricant to the piston Ensure that the piston is evenly lubricated and that you wipe away any excess remember only a fine
14. ealing thin walls and fine point making them easier to mount more flexible with no vortexing and improved precision Diamond Tips are free from defects especially at the orifice All surfaces are smooth and hydro phobic thereby avoiding the excessive retention of liquids that causes poor accuracy and a lack of precision e Mold and cavity references are marked on the collar ensuring the traceability for quality as surance purposes batch numbers appear on all packages bags and TipacksTM They form an air tight seal with the tip holder preventing the leaks that cause poor accuracy and a lack of precision They may be autoclaved at 121 C for 20 minutes at 0 1 MPa Diamond Filter Tips Filter tips are used when sample to sample sam ple to pipette orsample to operator contamination must be avoided Because of there unique construction Diamond Filter DF Tips may be autoclaved without risk of damage to the filters Gilson s sterilized Diamond Filter Tips are certified free of detectable RNases DNases DNA RNA and proteases 8 Table Tips to Use for Best Results Pipette Diamond Tips Volume Range U2 D10 DL10 DF10 DFL10 0 2 uL to 2 uL U10 D10 DL10 DF10 DFL10 1 uL to 10 pL U20 D200 DF30 2 uL to 20 pL DF30 20 uL to 30 uL U100 D200 DF100 20 uL to 100 pL DF100 20 uL to 100 pL U200 D200 DF200 20 uL to 200 pL U1000 D1000 DF1000 200 uL to 1 mL U5000 D5000 1 mL to 5 mL U10 ml D10 ml 1 mL t
15. etting not locked 6 7 Incorrect operator technique 9 12 Worn O ring 17 18 Connecting nut is loose 18 19 Damaged or corroded piston 18 19 Damaged tip holder 16 17 Tips fall off or don t fit Low quality tips 7 9 Damaged tip holder 16 17 Damaged tip ejector 16 17 No LCD display Pipette is not switched on 5 Battery has failed 5 20 21 ERR1 RECAL flashing Battery was changed when the 20 21 on display pipette was in the unlocked position Volume was not set to the correct 25 27 calibration volume when the calibration tool was plugged in Other Error Messages If any of the following occur contact your Gilson distributor Message Cause ERR2 Setting problems ERR3 Electronic problems microchip 15 EI PIPETMAN o E 12 MAINTENANCE You may perform the following operations clean or autoclave the parts specified under Cleaning and Decontamination replace the parts specified under Spare Parts lubricate the piston change the battery recalibrate the pipette Tip holder and Tip ejector These parts must be changed if they are ac cidentally damaged or attacked chemically You should also remove these parts for cleaning or decontamination purposes Changing the Tip ejector 1 Keep the tip ejector button depressed and grip the top of the tip ejector with the other hand 2 Gently rotate the tip ejector souinieiener Wise and separate its connector from the activating
16. o 10 mL 8 PIPETTING 1 Fit a new tip Gilson Diamond Tips give the best results see Specifications Plastic tips are for a single application they must not be cleaned for reuse Push the tip holder into the tip using a slight twisting motion to ensure a firm and airtight seal Pre rinse the tip Some liquids e g protein containing solutions and organic solvents can leave a film of liquid on the inside the wall of the tip pre rinse the tip to minimize any errors that may be related to this phenomenon Pre rinsing consists of aspirating the first vol ume of liquid and then dispensing it back into the same vessel or to waste Subsequent volumes that you pipette will have levels of accuracy and precision within specifications ESI PIPETMAN o E 3 Aspirate Press the push button to the first stop this corresponds to the set volume of liquid Hold the pipette vertically and immerse the tip in the liquid see table for immersion depth Release the push button slowly and smoothly to top position to aspirate the set volume of liquid Wait one second time depends on model see table then with draw the pipette tip from the liquid You may wipe any droplets away from the out side of the tip using a medical wipe however if you do so take care to avoid touching the tip s orifice 4 Dispense Place the end of the tip against the inside wall of the recipient vessel atanangle of 10 to40 Press
17. o the battery holder 7 Refit the copper clip 8 Reinsertthe battery holder into the battery compart ment cover 9 Refit the battery compartment cover Take care that the negative terminal of the battery is uppermost i e facing you 13 CLEANING AND DECONTAMINATION Pipetman Ultra is designed so that the parts normally in contact with liquid contaminants can easily be cleaned and decontaminated 21 ESI PIPETMAN o E Liquid must not enter the body handle of the pipette If you use chemical solutions for decontamination or detergents for cleaning other than specified below you should check with your supplier that the solution or detergent used does not attack any of the following materials stainless steel POM Polyoxymethylene PVDF Polyvinylidene Fluoride and PC Polycarbonate Cleaning The pipette must be cleaned as described below before itis decontaminated Soap solution is recom mended for cleaning Pipetman Ultra External 1 Remove the tip ejector see Changing the Tip ejector 2 Wipe the tip ejector with a soft cloth or lint free tissue impregnated with soap solution 3 Wipe the entire pipette with a soft cloth or lint free tissue impregnated with soap solution to remove all dirty marks Ifthe pipette is very dirty a brush with soft plastic bristles may be used 4 Wipe the entire pipette and the tip ejector with a soft cloth or lint free tissue impregnated with
18. o the second stop You should now be able to remove the O ring from the piston Some times the O ring may be found in the recess at the top end of the lower part of the tip holder EI PIPETMAN o E If required lubricate the piston see How to Lubri cate the Piston then fit a new O ring by sliding it onto the piston Reassemble the pipette The dimensions of the O ring vary according to the pipette model refer to Spare Parts for details Servicing the Piston You may remove the piston assembly to clean lubricate or change the piston The specifications of the pipette must be checked after changing the piston see Chapter 15 Removing the Piston 1 18 Set the volume to zero and lock the pipette Do not unlock the pipette or try to reset the volume while changing the piston assembly Remove the tip ejector and optionally the lower part of the tip holder I f you remove the lower part take care to remove the O ring as described in Changing the O ring Unscrew the connecting nut turn by hand counter clockwise Pull on the spring guide to remove the piston assembly from the body of the pipette separate the parts see below Tip holder Lower Tip holder Connecting Nut Upper oS Dm AAAA Piston holder Piston Spring Tapered Spring Guide gt For U5000 and U10 mi the connecting nut is combined with the upper part of the tip holder see
19. or User e Cycle counters see Chapter 5 from last volume setting to count the number of cycles in the current run from manufacture to count the number of cycles for servicing purposes e Flashing display when volume set is out of specifications e Indicator when pipette has been readjusted see Chapter 15 e Useful volume rangeis printed onthe push button 11 TROUBLESHOOTING gt fyou see RECAL flashing in the top right of the LCD you must readjust the pipette After user adjustment you will see RECAL but not flashing unless there is a problem with the pipette in which case you should contact your Gilson supplier Before returning any pipette ensure thatit is completely free of chemical biological or radioactive contamination You may be able to identify and to correct the problem by reference to the following table Symptom Possible Cause Refer to page Pipette is leaking sample Worn O ring 17 Pipette won t aspirate Worn O ring 17 Unscrewed lower part of tip holder 16 17 Damaged or corroded piston 18 19 Damaged tip holder 16 17 Improper repair or assembly 16 21 Connecting nut is loose 18 19 Noisy operation Piston needs lubricating 20 Pipette is inaccurate Improper repair or assembly 16 21 Unscrewed lower part of tip holder 16 17 Pipette is out of adjustment 25 27 Connecting nut is loose 18 19 Pipette is not precise Unscrewed lower part of tip holder 16 17 Volume s
20. our own procedures User should establish a routine for testing their pipettes at regular intervals taking into account the following factors frequency of use see cycle counters the nature and accuracy requirements of the liquids being pipetted the number of opera tors using the pipette and the number of cycles performed each time the pipette is used 25 ESI PIPETMAN o E Because Pipetman Ultra is designed with GLP in mind you may read the cycle counters after switching on the pipette Procedure Gravimetric Test Take 10 measurements using distilled water at the specified readjustment volume see below Use a balance which must be more accurate than the pipette to calculate the mean of the 10 measurements Model Readjustment Balance Volume pl sensitivity g U2 0 5 10 U10 1 10 U20 2 10 U100 20 10 U200 50 10 U1000 200 10 U5000 1000 10 U10 ml 1000 10 For example for an Ultra U200 the readjustment volume is 50 uL You set the LCD to show 50 uL but you calculate a mean volume of 48 uL a mean error of 2 uL Reset the pipette to show Readjust ment Volume minus Mean Error in this example 50 2 52 uL Open the battery compartment and plug in the user calibration tool as shown to reset the dis played volume to 50 uL I TheLCDwillshow RECAL in the volume setting window This indicates that the factory calibration has been modified To remove the RECAL sign and obtain a fac
21. pipette aside to stabilize at room tem perature Chemical Decontamination You may choose to decontaminate your pipette chemically in accordance with your own proce dures Whatever decontaminant you use check that it is compatible with the plastics used in the construction of the pipette see above Non immersible Parts 1 Wipe the body handle of the pipette with a soft cloth or lint free tissue impregnated with 23 ESI PIPETMAN o E the chosen decontaminant 2 Wipe the body of the pipette with a soft cloth or lint free tissue impregnated with distilled water or Sterile water Immersible Parts The following components only can be immersed in a decontaminant solution tip ejector tip holder both parts connecting nut piston including holder return spring and spring guide 1 Disassemble the pipette as described in Main tenance 2 Immerse the components in the de contaminant solution or wipe them according the instructions given by the manufacturer or supplier of the decontaminant 3 Rinse the individual components with distilled or sterile water 4 Leave the parts to dry by evaporation or wipe them with a clean lint free tissue or soft cloth 5 Lubricate the piston and reassemble the pipette according to the instructions given earlier in this chapter 14 LEAK TEST After servicing or repair and before calibration you are advised to perform a leak test as follows U2 to U
22. quids with temperatures dif ferent to the ambient temperature pre rinse the tip several times before use 8 For volatile solvents you should saturate the air cushion of your pipette by aspirating and dispensing the solvent repeatedly before as pirating the sample 9 Avoid pipetting acids or other corrosive liquids that emit vapors Extensive contact with corro sive fumes may corrode the pistons or damage the seal and tip holders ESI PIPETMAN o E 10 The pipette can be used between 4 C and 40 C but the specifications may vary see Chapter 16 for controlled conditions of use 11 Do not pipette liquids having temperatures above 70 C or below 4 C Extreme temperatures can affect accu racy and precision 9 PERSONALIZING YOUR PIPETTE Comfort of Tip ejector Before you start to pipette you can adjust the tip ejector button according to your preferences 1 Position the tip ejector button Simply rotate the tip ejector button to the most comfortable position left right or middle Tip ejector Button Top view Set for right handed user 7 S 2 Set the stroke by rotating the adjustment wheel until you find the position where itis most easy to activate the tip ejector You may wantto reset the stroke after fitting a different type or Stroke size of tip Adjustment Wheel I The tip ejector is adjustable in height to allow for different types of tip where for example the length
23. the push button slowly and smoothly to the first stop Wait for at least one second then press the push button to the second stop to expel any residual liquid from the tip Keep the push button pressed fully down and while removing the pipette draw the tip along the inside surface of the vessel Release the push button smoothly Table Immersion Depth and Wait Time Model Immersion Depth mm Wait Time seconds U2 1 1 U10 1 U20 2 3 1 U100 2 4 1 U200 2 4 1 U1000 2 4 2 3 U5000 3 6 4 5 U10 ml 5 7 4 5 5 Ejectthe tip by pressing firmly on the tip ejector button General Guidelines for Good Pipetting 1 Make sure that you operate the push button slowly and smoothly 2 When aspirating keep the tip at a constant depth below the surface of the liquid refer to the table Immersion Depth and Wait Time 3 Change the tip before aspirating a different liquid sample or reagent 4 Changethetip if a droplet remains atthe end of the tip from the previous pipetting operation 5 Each new tip should be pre rinsed with the liquid to be pipetted 6 Liquid should never enter the tip holder To prevent this press and release the push button slowly and smoothly never turn the pipette upside down never lay the pipette on its side when there is liquid in the tip ISS The use of a Gilson Carousel or Trio is recommended to store pipettes in the vertical position 7 When pipetting li
24. tory calibration send the pipette back to your Gilson distributor Remove the user calibration tool close the bat tery compartment and calculate the accuracy as 26 described in Procedure for Evaluating Accuracy and Precision of Gilson Pipettes whichis a more exhaustive gravimetric test for determining ac curacy and precision 16 SPECIFICATIONS Gilson Maximum Permissible Errors Maximum Permissible Errors Model Volume Gilson ISO 8655 Reference uL Systematic Random Systematic Random error uL error uL error uL error uL Min 0 2 0 024 lt 0012 008 lt 0 04 U2 0 5 0 025 lt 0012 0 08 lt 0 04 F21021 Max 2 0 030 lt 0014 0 08 lt 0 04 Min 1 00 0012 z012 0106 U10 5 20075 0030 012 0108 F21022 Max 10 0100 lt 0 040 012 lt 0 08 Min 2 0 10 lt 003 0 20 lt 0 10 U20 5 0 10 lt 004 020 lt 0 10 F21023 10 0 10 lt 005 2020 0 10 Max 20 020 lt 006 020 lt 0 10 Min 20 szos 010 2080 lt 00 U100 50 040 lt 012 080 lt 0 30 F21024 Max 100 080 lt 0 15 080 lt 0 30 Min 20 050 lt 020 160 lt 0 60 U200 50 050 lt 020 160 lt 0 60 F21025 100 080 lt 025 160 lt 0 60 Max 200 160 lt 030 160 lt 0 60 Min 200 3 lt 06 8 eu U1000 500 4 40 26 lt 3 0 F21026 Max 1000 8 lt 15 8 lt 3 0 Min 1000 12 lt 3 40 lt 15 U5000 2000 12 lt 5 40 lt 15 F21027 Max 5000 30 lt 8 40 lt 15 Min 1mL 30 lt 6 60 lt 30 U10ml 2mL 30
25. wly making sure not to overshoot the mark 4 Lock the volume push the thumbwheel down wards LCD shows LOCKED To unlock To reduce battery consumption lock the volume before storing Pipetman Ultra I Youare strongly advised to lock the volume to avoid accidentally changing the setting when pipetting e Theunits indicator flashes if you set a volume below 90 of the minimum recommended you can dispense an aspirated volume below the minimum recommended However in this case the specifications cannot be guaranteed e The volume indicator flashes if you try to set a volume above the maximum permitted you cannot pipette a volume that is greater than the maximum permitted 7 GILSON DIAMOND TIPS Gilson Diamond Tips are made to the highest specifications strict quality control is maintained throughout the manufacturing process Diamond Tips are used to calibrate Pipetman Ultra therefore for optimum performance you are strongly advised to use Gilson s Diamond Tips with your Pipetman Ultra ESI PIPETMAN o E Diamond Tips have the Gilson emblem engraved on their col lar ensuring that you have a genuine Gilson product To ensure accuracy and precision Gilson s Quality Assurance System focuses on the following critical parameters e Diamond Tips are made from pure polypropylene virgin metal free to avoid the possibility of contamination Optimized shape revised collar for optimum s
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