User Manual
Contents
1. 3 System Setting 3 1 In the Main menu interface move cursor or directly press the number key 7 to select System icon press Enter to enter System parameter setting interface Seem T ido ere 1 System date and time 3 Sort the list of files Time BR 51 37 Chronological order Date 11 01 01 O Alphabetical 2 Sound settings 4 Keyboard lock setting Keyboard sound Disable keyboard lock v Alarm sound O Enable keyboard lock v End alert sound Password 000000 Temp Reach sound 5 System diagnosis Press F1 savethe diagnosis to Udisk Module 0 2 amp 0 5ml Diagnose Default System Parameter Setting Interface 3 2 In the interface press cursor moving keys to select date and time of the system bip tone and ordering mode of file list etc press Enter to confirm the current item and press the number key to input specific values 3 3 Press Esc to close windows after the setting is over and the system will show the saving boxes after the user closes the window press Enter for saving or press Esc to give up 3 4 You can press F4 to choose default setting if has no specified requirements 4 Communication Settings 4 1 In the Main menu interface move cursor or directly press the numerical key 5 to select Communication press Enter to enter Communication setting interface Page 16 of 22 Com setting foot 11 01 01 12. 1 If the user inputs the value which is out of scope while setting a parameter the system will choose the default value and ignores the input value automatically 2 In the Main menu interface move the cursor to select File List icon select the file you want and press Enter to read the information Press ESC to exit Page 19 of 22 Chapter Four Maintaining and Trouble Shooting I Care and Maintenance of Instrument v The instrument shall be stored in the dry and ventilating environment keep away from direct sunlight or humidity or shadiness v Clean the wells of the module regularly else the residue left by the experiment will affect the accuracy of the experiment the corrosive solvent or cleaning agent is forbidden and the cotton swab stained with water free alcohol in appropriate amount is suggested to clean it and then use compressed air to clean each hole v Cotton cloth or non woven stained with appropriate pure water shall be used for cleaning if there is dirt or dust on the surface of the instrument organic solvent alcohol strong acid strong alkaline or other corrosive cleaning agent is not allowed in case of any damage to the instrument surface v After the experiment check the instrument and turn off the power and remove all the residues left by the experiment such as the water reaction solution and impurity in and out of the instrument to keep the instrument clean The protectiv
3. JKLMNOP QR STUVWXYZ Press Tab to switch small Press Enter to confirm input Press Esc to cancel input Module 0 280 5ml File Name Setting Interface 2 Edit 2 1 In the Main menu interface move cursor or directly press the number key 1 to select file icon press Enter to enter the interface of program file list 2 2 In the Program file list interface press lt or for Pg up Pg down press L or cursor to choose the file to be edited and move the 2 3 After selecting the file press F2 to enter the Unit segment amp step interface to edit the subsequent operation step is the same with 1 2 1 4 3 Copy 3 1 In the Main menu interface move cursor or directly press the number key 1 to select the file icon of File press Enter to enter the interface of Program file list 3 2 In the Program file list interface press lt or for Pg up Pg down press 0 or cursor to choose the file to be copied and move the 3 3 Press F4 after selecting the file name and the system will show Confirm press Enter to confirm copy or press Esc to cancel copy After confirming the system will enter File name setting interface and press F4 for saving after setting the file name 4 Delete 4 1 In the Main
4. Main menu interface move cursor or directly press the number key 4 to select the sub menu of Grads icon press Enter to enter Grads calculator interface Grads caculator foot 11 01 01 15 51 37 Center Temp EAIC Grads 10 C Temp 55 9 C 58 1 C 60 4 C 62 2 C 64 0C 65 1 C Grads Calculator Interface 99 ee 2 2 In the interface press O or to select items such as center temperature and Grads scope and press the number key to set specific values 2 3 The center temperature scope is 31 C 99 C the Grads scope is 1 C 15 C and the sum scope of the center temperature value and the Grade scope value is 30 C 100 C The user can check the temperature value of the 12 rows of the corresponding module after the setting is over 3 Help In the Main menu interface move cursor or directly press the number key 8 to select Help icon press Enter to enter Help interface And the ways to contact our company are listed in details in the interface IV 9700 Software 1 Introduction 1 1 9700 Software has a customer friendly interface 1 2 Any program in your computer may be copied to your machine via software You may save your time in edit the same program 1 3 Users may remote control and monitor machine s operating via a PC 2 You may see Details in 9700 Software user manual Page 18 of 22 V Others
5. menu interface move cursor or directly press the number key 1 to select the File icon press Enter to enter the interface of Program file list 4 2 In the Program file list interface press lt or for Pg up Pg down press O or cursor to choose the file to be deleted and move the 4 3 Press F3 after selecting the file and the system will show Confirm press Enter to confirm delete or press Esc to cancel delete II Parameter Setting 1 Running Parameter Setting 1 1 In the Main menu interface move cursor or directly press the number key 2 to select Set icon press Enter to enter Parameter setting interface Page 14 of 22 Parameter setting oor 11 01 01 15 51 37 1 Temp up down rate 3 Temp control mode Up rate Mc s Sample plate control Down rate 0 0 C S Test tube simulating 2 Low Temp preserve setting Disable Enable Preserve T 04 0 Module 0 2 amp 0 5ml Default Parameter Setting Interface 1 2 In the interface press or to select the temperature up down rate temperature control mode and to confirm if to select the low temperature preserve setting or not press Enter to confirm the current item and press the number key to input specific values The up down rate scope is 0 0 C S 3 0 C S and the low temperature scope of the low temperature preserv
6. windows Unit Segment amp Step Interface 1 3 In the Cycle parameter interface press F1 for PgUp and press F for PgDn Press cursor moving keys to choose the setting of temperature point and time scope and press number key to set the specific value of the selected setting and then press F4 then the system will enter File name interface New file gt Cycle Par oo1 11 01 01 15 51 37 C lt cyc 34 51 82 100 75 50 25 Temp 94 0 95 0 55 0 72 0 72 0 04 0 Time 02 00 00 30 00 30 00 30 05 00 05 00 Module 0 280 5ml Cycle Parameter Interface 1 4 The file name can only be composed by letters and numbers in the form of letters numbers or letters and numbers The setting of file name is to input the letters or numbers of the file name in order into the file name display area In the File name setting interface press Tab to switch letters press Cursor moving keys to select the letter and then press Enter to input the selected letter into the file name display area The user can press number key to input the number directly into the file name display area Press Esc to delete the wrong input information Press F4 for saving after the file name setting is over Page 13 of 22 New file gt Cycle Par oo1 11 01 01 15 51 37 e 4 CYC 34 4 iS au 100 AFile name PCROO1 a m m am 75 ABCDEFGHI
7. 6 51 37 Local Add USB CONFIGURATION DESCRIPTOR TYPE 0X02 CONFIG DESCRIPTOR LENGTH 0X002E USB INTERFACE DESCRIPTOR TYPE 0X04 NUM ENDPOINTS 0X04 USB CLASS CODE TEST CLASS DEVICE OXDC USB ENDPOINT 01 ACK OUT USB ENDPOINT 02 COMMAND IN USB ENDPOINT 03 DATA OUT USB ENDPOINT 04 DATA IN 4 2 In the interface press or V to set machine address or press numerical key directly The number you choose should be between 001 255 4 3 Press Esc to quit the communication setting interface after the setting and then the system will show the saving boxes press Enter for saving or press Esc to give up III Information Inquiry 1 Log Inquiry 1 1 In the Main menu interface move cursor or directly press the number key 6 to select the log icon press Enter to enter Log check interface If the number of the current files is lt 100 all the current file information will show in the interface If the number of the current files is gt 100 then information of the latest 100 files will show in the interface The information include file name running time and created date etc 1 2 Press lt or F3 or gt or F4 to page up or down saa om etr desta File name Running time Date saved PCR 81 11 01 01 PCR 82 11 01 02 Page 1 of 1 2 records Total times 2 Module 0 2 amp 0 5ml PgUp PgDn Log Inquiry Interface Page 17 of 22 2 Grads Query 2 1 In the
8. 9700 Thermo Cycler Index Chapter 1 Important Notice k Unpackins INS DECHON coss octo ED Tx coacceecceuateteascusanreeaaeinentcaseaetaatecusevinietewacauseter sete 1 EL Salet War min RI EU IIT 1 T Op ration Salely 24 ess sivewve Peers eu tuer OP ea A N cums ape cos deseteesawes 1 De Maintenance Safety ETT I 1 Chapter2 Brief of instrument I Introduction to PCR Teelinology eee eoa Senior eo ou ERPENEE USO a sever QR SeR UD tad bx sie ve Eva ONU RBERE enews 3 IE Instrument Features oi iiie ioi eia oa pie in etae detuvo ee duc cU ds vemaeta en aues Ea oit eL E ous 3 I Iurn on the Instrument dei eee neta aera doe Er UnEE TEE a Cue vET DEN dex Ru PPR CHER RI a 3 Di Din Elle pue RE 4 A iilud qe cicsaa setae siatsesiaw siod dalvaanicralacniueie eG ai adnan R T 4 M Wagiuclit a8 sia ona ee esl nins is ae eeu wae ae Chenier aula hee we aeae E OTSA 9 5 Working Conditions and Technical FeatureS ccccccccccccccccccccccccccccsccessseccccccssssseeerssees 11 Chapter3 Menu L File Qperati l sesione TT LUTTE 12 IE I rr i A EE T E OS 12 PAM Edil sia snore nie dive Seiad n a a aE E E n 14 Ds CODY scddasstewie T a a T a a a 14 Rr E T A A E EEA 14 H Parameter SetU lie faced ces ose vaidentcanausanaateeniadchewacie a teiaeeroatceereewteonesiont 14 I Running Parameter Sermo sesno E aat 14 PM EUIS PV n ese 15 Dat DV SUCHIN S CULLING ie e Cs Cy
9. amaged after receiving the instrument please keep the original package and contact your distributor as soon as possible so we can change a new one for you II Safety Warnings Please read the manual carefully before operating the instrument the wrong operation may be hazardous to the operator or the instruments itself CAUTION 1 Operation Safety The power cord for the instrument should be grounded reliably The instrument adopts three core grounding plug and should be connected with appropriate power socket Before connecting to the power you must make sure that the voltage and power load of the socket is in accordance with the capacity on the instrument s nameplate Please change the power cord with the same type if the skin of the cord is found to be broken Nothing is allowed to be place on the power cord and the cord shall not be placed in the area that may be easy approached by people Be sure to hold the plug tightly while inserting pulling of the cord directly is forbidden While the instrument is in operation the sample plate module and the heat lid will produce the high temperature so no part of human body is allowed to touch the metal module or the heat lid while the instrument is in operation in case of any scalding For the good ventilation no articles are allowed to be placed within 30cm around the instrument 2 Maintenance and Safety Page 1 of 22 The module and the heat lid of the in
10. ature in each of the PCR operation procedures even the most critical application Since the semiconductor application technology guarantees the best and constant temperature up and down rate the results of the gradient experiment can apply to the normal applications conveniently and accurately and reduce the working intensity of the trial experiments and thus increase the efficiency and quality of the experiments II Features 1 Turn on the Instrument After connecting related power turn on the power switch of the instrument and it will produce the sound of tick tack which means the instrument is in operation and now the screen will display the turn on interface See Figure 1 then the instrument will start automatic systematic inspection and then enter the stand by interface See Figure 2 if no fault 1s found If so this means the instrument is free for experimental operations now If the screen is dark or any other abnormal phenomena occur while turn on the instrument please cut off the power immediately and refer to Chapter 5 of the specifications for maintenance or contact the distribute or supplier for settlement Page 3 of 22 Twain name Tw sar Fextrabene Vw a Lid Grads 9700 Thermo Cycler 4 e C 2010 06 Ver 1 0 9 ar i m e Com Log System Help Module 0 2 amp 0 5ml Figure 1 Figure 2 2 Software A The system provides a sample plate for users to choose programming It not only can be set at a
11. certain fixed temperature point but also increase or decrease temperature among several set temperature points by a certain mode The changeable module design enables users to choose different experiment modules such as 96 0 2 ml 54 0 5 ml 96 0 2mL 77 0 5 ml and 384well according to their respective requirements and thus provides adequate experimental sample batches A The operation under low temperature is the basic characteristic of the advanced gene amplification instrument Its capacity to treat samples under low temperature and the PCR technology itself enable the sample treatment before PCR amplification and the sample reservation after PCR amplification to realize the automation of the whole operation process The system adopts advanced reliable semiconductor refrigeration scheme and thus provides high efficient temperature up and down speed and super wide temperature control scope A The 320 240 super large LCD can provide real time information of the instrument The key pad makes programming quick and convenient The system abandons the LINK mode of the traditional programming methods of the PCR instrument Instead it adopts its unique natural programming method The LINK mode makes the newcomers of the PCR easily to be confused during edit their own user files A The 30 C 100 C super wide gradient scope make user easily to design thier own test and thus obtain the experimental results of different temperature points in one expe
12. e cover shall be used to cover the instrument if it will not be used for a long time II Analysis for Common Faults and Solutions 1 Poor Experimental Results With the use of this instrument if the experimental result is poor then the following problems maybe exist Only for reference A The reaction article is wrong or the quantity is inadequate or the purity is not enough or the slight mononuclear chain area is incorrect B The denatured temperature is too high or too low so the user can add or reduce time according to the reaction amount C The annealing temperature is too high or too low D The reactant concentration is too high or too low E The preparation process is not specially treated F The time and temperature value in the programming is not suitable G The temperature of the sample or sample electrode is a little low whereas the base temperature is a little high H The user shall check if the reaction tube is placed well and if not little mineral oil can be used to coat the cone hole to increase heat conduction Page 20 of 22 I Deviation may occur to temperature control if it is not used for a long time if so the user may require the manufacturer to readjust the temperature control any personal testing or readjustment of the temperature control is not allowed 2 Common Defaults Analysis and Solutions The fuse is burnout Change for a new fuse 250V The screen is dark and there is n
13. e setting is 0 C 40 C 1 3 Press Esc to close windows after the setting is over and the system will show the saving boxes after the user closes the window press Enter for saving or press Esc to give up 1 4 You can press F4 to choose default setting 1f has no specified requirements 2 Parameter Setting 2 1 In the Main menu interface move cursor or directly press the number key 3 to select the lid icon for Heat lid setting press Enter to enter Heat lid setting interface Heat lid setting foot 11 01 01 15 51 37 8S Heat lid off Heatlid on when power on Q Heatlid on when file running File start run when heat lid reached set point Heatlid Temp 105 C Module 0 2 amp 0 5ml Default Heat Lid Setting Interface 2 2 In the interface press O orl to select items such as heat lid on or heat lid off heat lid on when power on file start run when heat lid reached set point etc Then press Enter to confirm the current item and press the number key to input specific values The heat lid temperature scope is 20 C 110 C Default value is 105 C 2 3 Press Esc to close windows after the setting is over and the system will show the saving boxes after the user closes the window press Enter for saving or press Esc to give up Page 15 of 22 2 4 You can press F4 to choose default setting if has specified requirements
14. eds to hold the module s handle vertically and insert the module in position then put the handle down to complete the operation Figure 9 i Figure 10 Step 1 Move the handle to the state vertical to the shell to make the module parts unlocked See Figure 9 Step 2 Move the module parts upward slightly and lay it in a safe place after the module parts are completely off the shell See Figure 10 Figure 11 Step 3 Choose the module parts of specifications needed and gently put them into the shell vertically Page 8 of 22 Press the handle downward Parallel to the shell SSS SS5 SSS Sees Se Figure 12 Figure 13 Step 4 After the module parts completely contact the shell press back the handle downward to make the magnetic steel on the handle and the shell mutually attract See Figure 12 Step 5 When the magnetic steel sticks and the handle is parallel to the shell the module parts and the shell will lock tight automatically Till now the replacement of module parts is over See Figure 13 The integrated module parts coupled with the intelligent module auto lock mechanism enable the user to conduct replacement in a faster and safer way In addition the option of module parts of various specifications enables the user to conduct experiments with tubes of different specifications in one set of instrument Notice Since a
15. g mode amp Help Module 0 2 amp 0 5ml Main Menu Interface 1 New 1 1 In the Main menu interface move the cursor or directly press the number key 1 to select File icon press Enter to enter the File list interface Ce eo POON aeaa File name Date created Page 01 of 01 Page 000 Files lt PageUp PageDown gt Press Tab to look U disk or Local U disk Module 0 2 amp 0 5ml New Edit Delete Copy File List Interface 1 2 Press F1 to enter the Segment amp step interface Press cursor moving keys to select the unit number unit segment number and cycle times of the file Press number key to input the specific value after selecting the setting If to set the extension function please press Tab to move the cursor to the 99 ee extension function area press or to move the cursor to choose Disable or Enable of grads temperature increment and time increment press Enter to confirm setting After the setting is over press F and the system will enter the Cycle parameter interface for the next step Page 12 of 22 New file gt Seg amp step foot 11 01 01 15 51 37 FileName Unnamed Grads 9 Disable Seg O Enable Seg 1 Step 1 Seg 2 Step 3 Cycle 34 Seg 3 Step 1 Seg 4 Step Time Inc Disable O Enable Temp Inc Disable O Enable Press Tab to switch
16. ic vas un 03A a ciara cress ois Siow a vin alain atnto aia bis MR EE RA PUPVMDUKRENI TAS Essen L CL LUDPE DESEE PRIN e sD MS 16 42 Communication SCNT corresse eo ow uds e ES RER EIDEM a Rad IO Dea 16 HT Information Ingy serisini EEE A EAE 17 LEM Pr PM in TE EE E E E E A 17 2 Grades InquiEy visiscieaie seic aina a A E E ea twweatiaes 18 SI dA nre T O E E aT 18 V 9700 SOLEWATC ansus a a E A 18 LAN SD PL 19 Chapter 4 Maintance and Trouble Shooting I Care and Maintenance of Instrument Leeececeeeeeeeeeee seen eene he restes setee reso 20 II Analysis for Common Faults and Solutions ccscccccscescsccecccscescscescscessesscscesces 20 1 Poor Experimental Results tino vue ER ANE Pere prr bePt sedentes visive tee ven eas tuu addu poU MUS 20 2 Common Defaults Analysis and Solutions sssssscsssscsscsccscccccccccccccccccccccccccccccsssssssseees 21 Chapter 5 Guarantee I Guarantee Content occ sors d codi rada paw ciii DIM PUN PU aasiw iene OU E OPRU TN Te ES 22 INE Goarantee SCODE ep iene eos esata as ewan es deen snieeods N eticcetane 22 Chapter One Important Notice I Unpacking Inspection Upon receiving 9700 gradient thermal cycler please open the packing box and check the packing list If the contents do not comply with the packing list please contact the distributor as soon as possible Notice If you find the package is seriously broken or the instrument is obviousl d
17. ll the lines of the instrument are connected by connectors the user shall use proportional strength while taking out or installing module parts and use skilled strength to shake them left and right No tough strength is allowed in case of any damage to connectors 4 Key Pad Operation panel in on the front side of the instrument Key pad is on the right and the screen is on the left Page 9 of 22 3 T a TE A 5 6 m Cycler 7 8 9 Run COM M ur On C Stop ve 9 Log System Help S M 2 Enter U X4 KIM J 7 ExtraGene f Key pad Screen Figure 14 MEME numerical keys for number setting and time or date input etc e arrow keys for moving cursor or turning page Tab Tab key for shifting windows letters and opening the grads o Rm Running key press the key to run the user file for data input Function keys having different functions in different menu interfaces such as back F1 F2 F3 F4 new delete cancel and save The user can operate according to the indications in the interface Cancel key for exiting from menus clearing dialogue box and canceling input Stop key press the key to stop the running file Enter key for confirming all dialogue box contents option and input Page 10 of 22 5 Working Conditions and Technical Features 5 1 Working Conditions of Instrument gt Environmen
18. nd practical characteristics and guarantees the safety and convenient operation Notice According to the instrument characteristics the open angle scope between upper lid and the shell is 0 90 please keep it within the scope to prevent any damage to the instrument 3 3 Heat Lid Position selector Frequently experiments need different reaction tubes that 1s use different module to match the tubes The design of the heat lid gear positions realizes the heat lid to match different sample plate modules just by turning the knob to the appropriate position Figure 5 Page 6 of 22 As the above figures indicate that the mark of 0 5 0 2 0 2 384 clockwise on the panel respectively corresponds to the gear position of tube 0 5ml 0 2ml round top 0 2ml flat top and 384sample plate modules If you need the 0 2mL tube flat top for experiment you only need change for the module with 0 2mL module See 3 4 Easily changeable module parts for details then switch the gear to the mark of 0 2 flat top and then you can start your experiment The design abandons the traditional practice of changing heat lid or even instrument Notice Refer to the structure of the instrument the turning range of the switch is between 0 5 to 384 do not try to turn the switch out of this range 3 4 Easily changeable Module Parts For the needs of users to use tubes of different specifications for experiment in the same instrument and fo
19. o tick 10A 05x20 sound after opening the power The switch is damaged Change the switch others Contact the supplier or manufacturer 1 The open circuit or short circuit of the cooling module sensor 2 The open circuit or short circuit of the The screen displays wrong box with the P Contact the supplier or ees W heat lid sensor sound of Tick manufacturer 3 The open circuit or short circuit of the radiator 4 others Block of vent Remove the clogs The slow down speed of the module or s a r The cryogenic chip is damaged or the Contact the supplier or failure of down i ventilator stop working manufacturer The slow up speed of the module or failure Contact the supplier or Damage to cryogenic chip of up manufacturer Damage to temperature sensor l l Contact the supplier or Failure of heating or cooling of the module 7 Damage to cryogenic chip manufacturer Damage to heating film l Contact the supplier or manufacturer The link line is loose Bad contact of the chip Contact th li The screen displays abnormal character aoe eee ee Damage to the chip manufacturer Contact the supplier or The press key of the panel does not function Damage to the press re manufacturer Page 21 of 22 Chapter Five Instrument Guarantee I Guarantee Contents 1 The instrument a common B type instrument for indoor uses is in accordance with the I type of the standards of GB9706 1 and has passed CE securi
20. r the convenience of users to change relevant parts directly and effectively the instrument adopts the portable design with auto lock function to make the instrument meet the experiments of different tubes on the condition of changing least parts which fully reflects the humanistic design of the instrument Module Components 1 Handle 2 Magnetic Steel 3 Frame 4 Module 5 Heat Sink Figure 6 The above figure indicates that the integrated module parts design of the instrument greatly improves operational convenience no matter in installation or replacement and the overall dimensions assembly process and installation method of all the modules of all specifications are the same The module parts of different specifications differ with modules corresponding to tubes of different specifications And this guarantees that users can change or install the module in a fast and high efficient way only by choosing the module with specification needed Handle Handle Shell Shell Figure 7 Figure 8 Page 7 of 22 Figure 7 shows how to replace the module unit When changing the module the user shall pull the handle upward and when the handle and the shell are in a vertical state the module will be unlocked and then the user can easily take out the module parts by pull the handle vertically Figure 8 shows how to insert the module when the user confirm the module parts of certain specifications needed the user only ne
21. riment This greatly reduces the experimental cost and intensity The gradient temperature control technique can have very flexible temperature control point to guarantee the realization of the experiment purpose in a high efficient way especially for the experiments which requires accurate but uncertain temperature 3 Structure 3 1 Shape View Page 4 of 22 1 Ventilation Window 5 Upper Lid 9 Power Switch 13 USB Interface 17 LOGO 2 Base 6 Control Panel 10 Power Socket 14 JTEG Interface 18 Lid Wrench 3 Foot Rest 7 Screen 11 Fuse Socket 15 Shell 19 Module selecting knob Figure 3 3 2 Upper Lid may Stop at Any Angle when Open It 4 Ventilation Window 8 Key Pad 12 Network Interface 16 Ventilation Window 20 Module Selecting Indicator This type of instrument takes a leading role in adopting the hinge joint technology enabling the upper lid of the instrument to stay at any angle according to requirement and solving such problems brought by the traditional method of relying on spring elasticity to open the lid as unreliability instability large inertia and short life etc Page 5 of 22 Figure 4 As indicated in the above two figures when the wrench is raised the buckle will be released and then the user can set the upper lid at any angle according to their requirement Humanistic structure endows the product more beautiful a
22. strument need regular cleaning to guarantee the precision of the experiment Before cleaning the sample plate module is suggested to be taken out for soft scrubbing by the clean soft cloth stained with the pure alcohol in appropriate amount No corrosive cleaning agent is allowed for use and dripping the cleaning agent into the taper hole is forbidden in case of any damage to the instrument And the sample plate module should be put back into the instrument after cleaning Please turn off the power at once and stop the experiment and then immediately contact the distributor or the professional maintenance personnel in case of any occurrence of the following situations Any reagent rain water or other liquid enters into the instrument The instrument falls from a high place or the instrument shell is damaged The operating instrument produces the abnormal noise or the pungent odor The features of the instrument changes apparently such as failures of startup or shutdown or failure to run user program etc Notice For your personal safety and the normal operation of the instrument please do not disassemble the instrument by yourself Any such behavior may endanger your safety and deprive your guarantee right of the instrument Statement This user manual may not includ all information of the machine We may improve or change package performance structure appearance of product at any time without notice If you ha
23. t temperature 10 C 30 C Relative humidity lt 70 Working voltage Please properly use the voltage to meet local charge 5 2 Storage Conditions of the Instrument gt Environment temperature 20 C 55 C gt Relative humidity lt 80 Notice In order to guarantee the normal uses of the instrument and the accuracy of experimental results please run or store the instrument according to prescribed working conditions and storage conditions Or the wrong operation will damage the instrument and even personal safety 5 3 Technical Features Type 9700 Sample size 96 0 2mL A 54 0 5mL B 96 0 2mL 77 0 5mL C 384well D Temperature scope 0 C 99 9 C Rt lt 30 C Max temperature up rate gt 3 C s Max temperature down rate 22 8 C s Temperature uniformity lt 0 4 C Temperature accuracy lt 0 1 C Grads 30 99 C Grads width 2C 30 C Lid temperature 20 C 110 C Temperature control mode Block calculated Program storage 200 Maximum cyclic number 99 Graphic interface 5 7inch 320 240pels LCD Communication interface USB2 0 Overall dimension 380mm L 270mm W 250mm H Net weight 7 2kg WON NONO NON VV VV VV NON VV WV Page 11 of 22 Chapter Three Menu I File Operation The instrument enter the main menu interface after being turned on the interface is divided into the information bar main display area and status bar And the cursor displays in the main display area in reversin
24. ty authentication 2 Within one month since the date of the delivery for any damage caused by materials or manufacturing happening to the instrument we will change it 3 Within twenty four months since the date of the delivery for any damage caused by materials or manufacturing happening to the instrument our company will provide the guarantee service Within the warranty period the company will repair or change the instrument proved to be defective 4 The user shall send the product to the maintenance department confirmed by our company The user shall pay the expense occurred by transferring the instrument to the maintenance department And the company shall pay the expense occurred by transferring the instrument back to the user 5 As to the repair beyond the warranty period the company will charge the relevant maintenance fees II Guarantee Scope 1 Under normal working conditions if any damage or default to the instrument is caused by materials installation or other flaws of the instrument itself our company shall provide the maintenance services according to the above mentioned maintenance contents 2 If the user fails to operate according to the standard specified or uses it under the inappropriate conditions or arbitrarily dismantles or repairs the instrument without authorization then any damage or default thus occurred shall not be contained in the maintenance scope of our company Page 22 of 22
25. ve any problem please contact with us Page 2 of 22 Chapter Two Brief of Instrument I Introduction to PCR Technology o Polymerase Chain Reaction PCR is the external body nucleic acid amplification technology which has been developing since middle 1980s This technology has advantages such as high specificity sensitivity high yield swiftness convenience good repetition and easy automation etc the technology can amplify the target gene to be studied or a certain DNA segment in a tube by ten thousand even millions of times within a few hours for the direct observation and judgment by naked eyes it can amplify the adequate DNA from a piece of hair a drop of blood even a cell for analysis and detection which needs days or weeks to be completed in the past can be completed within hours with the PCR technology now The PCR technology is the innovation and milestone in the biomedical field The PCR technology applies to such research fields as immunology human genome engineering forensic science oncology tissue amp group biology paleontology zoology and botany etc and the PCR detection in the clinical diagnosis fields such as virus tumor and genetic diseases etc o Becoming the first choice of the optimal reaction condition the gradient PCR has been the standard of the modern molecular biological lab A random remote controlled gradient temperature can optimize not only the annealing temperature but also all the temper
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