User Manual
Contents
1. User selectable at 200 400 800 and 1600 mN 0 01 mN Semi automatic Built in Ambient temp to 50 C 0 1 C External Force mN 1 0 V full scale calibrated Direct transducer output uncalibrated 0 26 mV mN 10 RS232 RS485 2 Hz 100 to 240 VAC auto 50 60 Hz The rectangle chamber allows a maximum segment length of 15 mm and can also be used for study of various organs Internal chamber dimensions Length 45 mm Depth 13 5 mm Width 11 mm Supports The clamp supports have a width of 3 mm Another option is vertical hook supports where the muscle strip ends are mounted with suture wire DMT Muscle strip myograph system Model 800MS DANISH M10 T aHnNo P G DMT A S Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Everwin Gardens Rm 502 Block B 521 Wanping Nan Lu Shanghai 200030 China Tel 86 0 21 64869685 Fax 86 0 21 64280591 www dmt asia com sales dmt asia com support dmt asia com DMT USA Inc 1201 Peachtree Street 400 Colony Square Suite 200 630 Atlanta GA 30361 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com2. 15 Chapter 3 The Myo Interface rrnnnnnnnnnnnvrnnnnnnnvevnnnnnnnnnnvnnnnnnnnnnvennnnnnnvvennnnnnnnnnnr 16 3 1 Turning on the Myo Interface sese enne E nnne nnne nnne nennt 16 3 2 0 Main menus and SUDMENUS inicia a iii 16 Chapter 4 The muscle strip myograph unit e 20 4 1 Calibration of force transducer 222 ES ERE REEL E nane nnne nan naar ES 20 4 1 1 Principles of weight callDr tion retten neret eren 20 4 1 2 Weight calibration procedure mios unt rnnt ni ena us anne been 21 4 2 Checking or replacing force transducer essen 22 4 21 Simple transducer check een rri iaa 22 4 2 2 Force transducer replace Me n s ssccccecsesscceecsesssesecesessnaeecesesscansessasseanseseassesneeseeesessesees 23 4 3 Myograph MAINTENANCE sissviecssccsccsscavcisctescasieeasesiuedeccsstachessotaneesetaseessmencesdetaeeesstaeeesce 23 43 1 Myograph chamber PIPES semana 23 43 2 ROrce trans d Eas ses ecc ves orien vase rr Erro Pen dr ET 24 Z 3 3 ScreWADIGad meca 24 4 3 4 Myograph Cleaning ii toi coro uttter sas caste iieri rni aces ipe idae dnb 25 4 3 5 Mounting a muscle strip on the 800MS ic narrar 27 Appendix 1 Terms of warranty crece REEL SEERE anna 28 Appendix 2 Service cheCk r rernnrrnnnvrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnernnevennnnnnnnnnnnnnnnnnnnnnn 29 Appendix 3 Shipping instructions rrrvssrrnnnnnnnnnnnnnnnnnunnnnnv
3. EC must further enclose a pro forma invoice stating that the box contains goods being returned for repair or service If arranging transportation through a courier please keep in mind the high value of the myograph system and that a standard insurance provided by the courier in most cases is insufficient to cover damage or loss of the myograph system In most cases an additional insurance coverage is needed DMT Muscle strip myograph system Model 800MS Appendix 4 Myograph accessories and spare parts This appendix contains a complete register of equipment needed to set up a Muscle Strip Myograph System In addition the chapter contains a list of special 800MS myo graph accessories and spare parts Besides the main focus on development and manufacturing DMT has specialised in offering our customers first class laboratory equipment needed for a Muscle Strip Myograph setup at very competitive prices Please contact the DMT Sales Depart ment for further product information and prices A4 1 General myograph equipment This section contains a complete checklist of laboratory equipment needed when set ting up a Muscle Strip Myograph system Dissection stereo microscope Including ocular micrometer and stage microm eter DMT recommends the Zeiss Stemi 2000 Stereo Microscope Mounting forceps DMT Item DF 3000 DMT recommends Dumont Medical No 5 tip 0 10 mm x 0 06 mm Dissection scissors DMT Item DS 1000 DMT recommends Geude
4. Range 200mN Serial port submenu For digital storage of data the interface has an integrated RS232 port and two RS485 serial ports The RS232 serial port is used when a single system is connected to a computer and the RS485 serial ports are used for multiple connections up to four systems linked together allowing 16 force channels and four temperature readings By default the system is set to use RS232 and functions for the serial settings are found in Setup submenu 10 To switch between the two outputs use the and buttons Using the enclosed serial cable with the mounted adapter the 9 pin plug is connected to the RS232 plug of the interface and the 25 pin plug connected to the serial port COM port on the computer RS232 Serial Port This serial communication protocol must be used when only one system is connected to one PC gt Serial port RS232 RS485 Serial Port This serial communication protocol must be used when mul tiple systems are linked together In order to use this feature an external or internal signal converter that is half duplex and toggles RTS signals are required The converter if external must be placed between the computer and the first myograph Note For further information please contact DMT gt Serial port RS485 Linking multiple systems Connect a serial cable to the computer and to the signal con verter Connect a second serial cable to the other end of the er signal conver
5. critical life care use and should never be used for these purposes nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap Do not open the unit the internal electronics pose a risk of electric shock Do not use this apparatus near water e To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus Do not block any ventilation openings Install in accordance with the manufacturer s instructions Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time This apparatus must be grounded e Useathree wire grounding type cord similar to the one supplied with the product e Do not defeat the safety purpose of the polarized or grounding type plug A polarized plug has two flat blades one being wider than the other A grounding type plug has two blades and a third round grounding pin The wide blade or the third prong is provided for your safety If the provided plug does not fit into your outlet consult an electrician for replacement of the obsolete outlet e Be advised that different operating voltages require the use of different types of line cord and attachment plu
6. when applying a certain amount of weight The magnitude of the torque T about a point of rotation P is defined by Tot t r F sing where r is the distance from the point of rotation to the point on the object where the force F is acting with the angle of 0 Applying the weight on the pan arm creates a net torque acting at the center of grav ity resulting in a force F acting on the force transducer The following two equations describe the forces working in the weight calibration system LPer snb e og 8 sind weigt i 2 T I F sin where r is the length of the pan arm F is the force acting on the pan arm when applying the weight F is equal acceleration of gravity times the mass of the weight r IS the length of the transducer arm and F is the force acting on the force trans ducer The net torque acting at center of gravity is constant for the weight calibration setup which makes equation 1 and 2 equal making it possible to calculate the force acting on the force transducer DMT Muscle strip myograph system Model 800MS Fa Es SINO Fes Ma B SING F r m T PA sind weigt i 8 ra SING As the length of the pan arm is 2 cm the length of the transducer arm is 4 cm the weight is 2 g both angles are 90 and the acceleration of gravity is 9 81 ms the force acting on the force transducer is F 2cm 2 g 9 81 ms sin90 4 cm sin90 F 9
7. 5 Electrical equipment for measurement control and laboratory use EMC requirements Part 2 6 Particular requirements In vitro diagnostic IVD medical equipment With reference to regulations in the following direc tives 2006 95 EC 89 336 EEC User manual 800MS Certificate of Conformity Contents Trademarks ECC EEN 1 3 AAA iaarAkesiA kasa sns aA Ases AnA SENAN sir A EEs RAS Esas Seaan Esain ia 4 A E 5 Jj udA lp 6 e ER 6 Certificate of Conformity r sssvrrrnnnnnnvnnnennnnnnnvnnnnnnnnnnvnnnnnnnnnevnnennnnnnnvnennnnnnnneennnne 7 honlpAnILXurgrlm Ge 9 Unpacking the myograph system eee eeeeseeeee eee ee eene nnne nnn nnn 10 Chapter 1 System overview ssrrrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnvevevenr 11 1 1 Myo Interface front panel iecore re ozi s treo ciere cnn rea av eraot e reap o corona RRES ENED E NE 11 1 2 Myo interface rear paneleran REEE EE ARRE 11 1 3 Muscle Strip Myograph Unit uannvveernnvvernennvvenennvvernenvveenennvnenensvvensennveesensnveesennneeenn 12 Chapter 2 Setting up wssscscccicscccciccecsscsnicssnecnsscctnecescesseedcseccseeccnscccnecsneesstenaresss 13 2 1 The complete muscle strip 800MS myograph system esses 13 2 2 Settirig up Step by Step corio ocn eta x aoo cua cu cn oen e Lacu RR Roco cu Rogues 13 2 3 The first weight calibration
8. 6127800 40 mm funnels DMT Item F 2000 DMT Muscle strip myograph system Model 800MS Appendix 5 Fuse replacement The main fuse of the myograph system is placed inside the power inlet on the Myo Interface When a fuse blows and needs to be changed it is imperative that the replacement fuse is equal to the one blown The 610M system uses T1 6A 250 V 6 3 x 32 mm DMT recommends that both fuses in the fuse block are changed at the same time as it can be difficult to determine which fuse is blown To replace the fuses Use a small screwdriver to open the voltage selector block Remove the red fuse block Remove the existing fuses Insert the new fuses Replace the fuse block back into the voltage selector block Note ensure that the correct voltage for your country is displayed p dem p Voltage selector block User manual 800MS Appendix 5 33 34 Appendix 6 System specifications Muscle strips Chamber Chamber material Chamber volume Chamber suction Chamber gassing Chamber cover Force range Force resolution Weight calibration Heating Temp range Temp resolution Temp probe Output reading Recorder output Recorder output Standard Serial output Voltage Muscle unit gt 3 mm 15 mm Four individual chambers Acid resistant stainless steel Max 4 mL Manual or automatic Individually controlled per chamber by needle valves With electrodes optional
9. 81 gram m s As 1N is equal 1 kg m s F is equal to F 9 81 mN 4 1 2 Weight calibration procedure The section contains a complete step by step description of how to weight calibrate the force transducer 1 Place the units on the interface plug in the cables and fill the chambers with double distilled water Place the calibration bridge balance and weight on the first myograph unit allow ing it to be pre warmed together with the myograph unit it is important that the calibration kit is warm Turn on the heating in the Heat menu of the Myo Inter face The system will typically reach the target set temperature by default 379C after 20 minutes Place the calibration bridge balance and weight on myograph unit 1 as illus trated in fig 4 1 Make sure that the tip of the transducer arm on the balance is positioned protruding behind the clamp support Carefully move the calibration bridge until the tip of the transducer arm is hanging free which means it does not touch the side of the chamber or the support inset enlargement in fig 4 1 Note The weight should not be placed on the balance yet Go to the Calibration menu on the Myo Interface Ensure Calibrate 1 is active the symbol is displayed to the left use the F1 button to scroll through the list When the relative force reading in the display is stable press F2 to proceed with calibration of force transducer 1 Carefully place the 2 g weight on the pan as p
10. A China Tel 45 87411100 Tel 86 0 21 64869685 Tel 1 770 612 8014 Fax 45 87 411101 Fax 86 0 21 64280591 Fax 1 678 302 7013 www dmt dk www dmt asia com www dmt usa com sales dmt dk sales dmt asia com sales dmt usa com support dmt dk support dmt asia com support dmt usa com User manual 800MS Trademarks Introduction The 800MS muscle strip myograph system represents the first state of the art four channel myograph system for muscle strips of up to 15 mm in length DMT developed this system for simultaneous measurement of up to four isolated muscle preparations mounted as strips between clamp supports Several types of mounting supports exist depending on the user s preferences and customized Mounting supports can be made upon request Moreover because of the rectangular chamber the muscle unit can also be used for the study of larger strips of various other organs including larger pieces of smooth Muscle Stimulation electrodes are available built into the chamber cover optional and can be used to activate the Muscle via field stimulation from a pulse train stimulator such as the DMT CS200 The muscle bath unit is constructed of aluminium while the centrally located rectangular chamber is stainless steel for easy cleaning The segment clamp supports are positioned in the chamber with one side attached to the force transducer and the other side to a micrometer The micrometers permit the accurate setting of muscle l
11. Muscle Strip Myograph System Model 800MS I DANISH MYO TECHNOL OG User Manual Version 2 2 DANISA M10 TECHNOL 0DG DMT Muscle strip myograph system Model 800MS Muscle Strip Myograph System Model 800MS User manual Trademarks PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 25 are trademarks of ADInstruments Pty Ltd Pentium is a registered trademark of the Intel Corporation Windows Windows 95 Windows 98 Windows ME Windows NT Windows 2000 and Windows XP are registered trademarks of Microsoft Corporation All other trademarks are the properties of their respective owners DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the DMT muscle strip myograph system Model 800MS v 2 2 Document Number 800MS UG2 2A No part of this document may be reproduced by any means without the prior written permission of DMT Copyright O 2008 DMT A S MI DANISH MYO TECHNOLOGY DMT A S DMT Asia DMT USA Inc Skejbyparken 152 Everwin Gardens 1201 Peachtree Street DK 8200 Aarhus N Rm 502 Block B 400 Colony Square Suite 200 630 Denmark 521 Wanping Nan Lu Atlanta GA 30361 Shanghai 200030 US
12. able in spite of a recent weight calibration then repeat the weight calibration and note down the relative force reading values shown in the Calibration menu on the Myo Interface e fthe value is O or above 6500 then the force transducer is broken and needs to DMT Muscle strip myograph system Model 800MS be changed e If the value is between 1 499 or 3001 6250 then contact DMT for further instructions Important If at any time the message OFF is displayed in the Force menu on the Myo Inter face this indicates that the force transducer is broken and must be replaced In this instance or in case of other problems related to the force transducer please contact DMT for further instruction and advice 4 2 2 Force transducer replacement If the force transducer is broken and needs to be changed please follow this step by step replacement procedure carefully 1 Disconnect the myograph unit from the Myo Interface grey cable 2 Remove the myograph clamp support connected to the transducer pin 3 Turn the myograph unit upside down and remove the bottom plate by loosen ing the two screws A B as illustrated in fig 4 2 Carefully disconnect the force transducer plug and remove the old transducer Note how the plug is connected to the old force transducer to prevent incorrect connection of the new force transducer 4 Remove any remaining grease from the transducer pin left inside of the trans ducer compartment of t
13. acquisition and analysis system optional I Direct PC connection Connect the Myo Interface to one of the COM ports on the PC using a serial cable PowerLab optional Connect the Myo Interface to the PowerLab unit using BNC cables Connect Rec 1 on the Myo Interface to Input 1 on the PowerLab Rec 2 to Input 2 etc Connect the PowerLab unit to one of the USB ports on the PC using the USB cable delivered with the PowerLab system Follow the DMT Quick Guide in structions delivered with the PowerLab system to install the PowerLab driver and Chart software on the PC User manual 800MS Chapter 2 13 Note there are two types of analog recorder outputs on the rear panel of the 800MS system The top four provide a direct raw voltage signal from the transducer uncalibrated and unfiltered The bottom four BNC connections provide a calibrated signal with a fixed 1 V output where the voltage output corresponds to the mN setting in the Myo Interface Recorder menu described in Chapter 3 2 Gas supply The gas supply tubes must be connected in order to have the solution aerated fig 2 2 Connect the main gas supply on the back of the interface Each myo graph unit has a small diameter silicone tube which should be connected to the gas tube from the rear panel of the interface it has a stainless steel tube The needle valves are used to regulate the level of bubbling to each chamber Each needle valve has a lock device attached Note T
14. alibration of the transducers gt Calibrate 1 using the calibration kit provided with the 610M system The Calibrate 2 F1 button changes which transducer is active in the list Slct Se stands for select Pressing the F2 button will begin the calibra tion process for the force transducer selected indicated by gt Calibration of the transducers is described in full in Chapter 4 Setup menu The Setup menu provides access to submenus which are used to change the gain setting serial port connection and link if multiple systems are connected To enter the desired submenu use the or buttons and when the appropriate number is shown the submenu is activated by pressing F2 Whenever any changes have been made one must return to the Setup menu using 1 for the changes to take effect User manual 800MS Chapter 3 17 18 Valve delay submenu The valve delay submenu is found under Setup submenu 3 By default the valve delay is set to 1 second the available delay range is 1 to 99 seconds The valve delay is the time the valve is open once you have released the valve button on the front panel The delay time is changed using the and buttons Valve delay 1sec Force range submenu The force range submenu is found in Setup submenu 7 By default the force range is set at 200 mN The available rang es are 200 400 800 and 1600 mN The force range is changed using the and buttons Force
15. ast 15 minutes of the incubation period 3 Wash the myograph chamber and supports several times with double distilled water 4 Incubate the myograph chamber with 9696 ethanol for 10 minutes while continu ing the mechanical cleaning with a swab stick 5 Remove the ethanol solution and wash a few times with double distilled water Incubate the myograph chamber and supports with an 8 acetic acid solution for 10 minutes and continue the mechanical cleaning with a swab stick 6 Wash the myograph chamber and supports several times with double distilled water User manual 800MS Chapter 4 25 26 Important Notes n exceptional cases to make sure that all surfaces are cleaned it may be neces sary to remove the supports so that they are cleaned separately from the myo graph chamber DMT Muscle strip myograph system Model 800MS 4 3 5 Mounting a muscle strip on the 800MS 3 E gt O 1 Starting position 2 Loosen the screws on the clamp support 3 Turn the upper part of the support to expose the lower part 4 Place the muscle strip onto the lower part of the clamp support 5 Turn the top part back into position so that the muscle strip is clamped between the upper and lower parts 6 Secure the screws User manual 800MS Chapter 4 27 28 Appendix 1 Terms of warranty Warranty DMT A S warrants to the original user that myograph systems manufactured by DMT A S will be free fro
16. display is depicted to the right where myograph unit 1 is unplugged LEGER Force 3 1 87 mN To replace the unit firstly press the arrow displayed in the active A line The display now changes to Now mount myo No 1 Put the chamber unit in place on the interface always place chamber NOW MOUNT MYO NO 1 unit one at No 1 on the interface unit two on No 2 and so forth ms Tem and plug the chamber cable into the corresponding input on the p c interface s rear panel If the unit is not plugged into the interface within 10 seconds then the arrow key should be pressed again the interface will display the previous message Once the unit is connected the active line will again display a force reading Zero menu This menu is used to zero the output of the transducers The F1 button changes which transducer is active in the list Slct stands FASE for select Pressing the F2 button will zero the selected trans ducer indicated by gt F2 Zero DMT Muscle strip myograph system Model 800MS After selecting F2 the selected transducer will be zeroed and the display will automatically return to the force menu Re en ter the zero menu using the arrow down button choose the next transducer with the F1 button and zero with F2 Repeat this procedure until all transducers have been zeroed To simultaneously zero all channels enter the zero menu and press the right arrow and F2 at same time Note there shou
17. e be handled with the utmost care One of the jaws in each myograph is connected to the transducer pin The transducer pin is attached to the myograph mounting supports and is located outside of the chamber fig 4 3 Despite there being no direct contact to the salt solution in the chamber there is a risk for evaporating solution to deposit calcium in the pinhole The hole running into the transducer house the hole is therefore filled with high vacuum grease Figure 4 3 Transducer pin holes to be sealed up with high vacuum grease 3 x As part of daily maintenance it is very important to inspect the greasing of the trans ducer hole before starting any experiment Insufficient greasing permits salts and fluid to enter thereby causing damage and malfunction of the force transducer 4 3 3 Screw thread Check the screw thread for grease at least once a week In case of insufficient lubri cation grease the thread with the original enclosed grease for screw thread at the places marked by the arrow in fig 4 4 Figure 4 4 Greasing point for the screw thread DMT Muscle strip myograph system Model 800MS 4 3 4 Myograph cleaning DMT strongly recommends that the myograph chamber and surroundings should be cleaned after each experiment After an experiment use the following procedure to clean the myograph chamber and supports 1 Fillthe myograph chamber to the edge with an 8 acetic acid solution and allow itto work f
18. e myograph system Please take a few Minutes to carefully inspect your new Muscle strip myograph system for damage which may have occurred during handling and shipping If you suspect any kind of damage please contact DMT immediately and the matter will be pursued soon as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the muscle strip myograph system In case of transport and the original packing material is unavailable please contact DMT Sales Department for advice and packing instructions After unpacking your new system please use the following list to check that the system is complete e 1 interface unit 4chamber units with mounted clamp supports Achamber covers 1 external temperature probe 1 power cord e 1calibration kit including bridge balance and 2 g weight e 1 tube of high vacuum grease 1 tube of grease for screw thread e 2 Allen keys e 1 small screwdriver 1 user manual The shape of the AC plug varies by country be sure that the plug has the right shape for your location DMT Muscle strip myograph system Model 800MS Chapter 1 System overview 1 1 Myo Interface front panel Sameie i EE um 2 Power indicator d Valve keys Myo Interface display Function keys 1 2 Myo Interface rear panel 4 Recorder outputs uncalibrated e Reg
19. ength and the sensitive force transducers measure the resulting isometric muscle tension Each unit has individually controlled gas inflow and suction The system is automatically heated to a given set temperature above ambient Following mounting and equilibration the optimal length tension relationship for the muscle can be determined and the muscle length set to this value with the micrometer The length of the muscle is kept constant during the experiment i e the muscle is examined under isometric conditions Compounds are added directly to the chamber and muscle tension is monitored for chronotropic or intropic effects of the compound The bath fluid can be sampled for metabolic factors This myograph is well suited for investigations on muscle reactivity and multiple units can be arranged conveniently side by side The force readings are available for external data acquisition via either calibrated filtered or uncalibrated unfiltered analog outputs or in digital format via a serial connection Furthermore due to its four channel design the 800MS is ideal for work requiring high throughput screening HTS such as drug testing or in experiments where comparison of muscle preparations from control and treated or gene modified animals is required DMT Muscle Strip Myograph System Model 800MS Safety The muscle strip myograph system has been designed for use only in teaching and research applications It is not intended for clinical or
20. ennvevevevennnennnnnnnnnnnn 30 Appendix 4 Myograph accessories and spare parts 31 Appendix 5 Fuse replacement rrrrrnrrnnnnnnnnnnnnnnnnnnnnnnnnnnnunevevevennennnnnnnnnnnnnnnnnnnn 33 Appendix 6 System specifications eeeeeer ee eeeen nene 34 DMT Muscle strip myograph system Model 800MS About this Manual gt gt This manual contains a complete list of procedures describing how to install maintain and get started using the muscle strip myograph system Model 800MS version 2 2 Chapter 1 provides an overview of the construction and basic features of the Myo In terface and the muscle strip myograph unit Chapter 2 describes step by step how to set up a complete muscle strip myograph system Model 800MS including accessories Chapter 3 is a complete manual to the Myo Interface The chapter describes in detail the construction of the menu system and how to use all the features of the muscle Strip Myograph System Model 800MS Chapter 4 contains procedures describing general as well as daily maintenance of the myograph unit e g adjustment of supports weight calibration of the force trans ducer and cleaning instructions Appendices contain various additional information about myograph service shipping instructions system accessories and spare parts system specifications and fuse replacement User manual 800MS Contents 10 Unpacking th
21. graph Service check includes a complete separation for inspection of all mechanical and electronic parts The myograph is then reassembled adjusted and finally all electronic and mechanical parts are tested Please note that the service does not include replacement of transducers or any other required spare parts Please contact DMT for information about prices User manual 800MS Appendix 2 29 30 Appendix 3 Shipping instructions If the myograph system needs to be sending back for service or repair please read the following shipping instructions very carefully Before you start packing the myograph system please remember that you are dealing with very delicate equipment and therefore care must be taken DMT recommends that each part of the myograph system be wrapped individually i e with bubble wrap and placed together in a large box preferable the box you once received the myo graph system in Place the wrapped items in the middle of the box and fill out the surroundings with chips of expanded polystyrene Important Before closing the box make sure that no enclosed items can be shaken around as transport by road or air from time to time can be quite roughly Address the box to DMT A S Skejbyparken 152 DK 8200 Aarhus N Denmark Make sure that all four sides of the box are marked fragile or similar Make an indi cation on the top of the box that it contains goods returned for repair service Customers outside the
22. gs Check the voltage in your area and use the correct type See the table below Line plug according to standard 110 125V UL817 and CSA C22 2 No 42 220 230V CEE 7 page VII SR section 107 2 D1 IEC 83 page C4 240 V BS 1363 of 1984 Specification for 13A fused plugs and switched and unswitched socket outlets Protect the power cord from being walked on or pinched particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped User manual 800MS Safety EMC EMI This equipment has been tested and found to comply with the limits for a Class B Digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in residential installations This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception w
23. he myograph unit Also clean the hole leading from the transducer compartment to the myograph chamber Plugthe replacement transducer in Replace the bottom plate and tighten the two Allen screws Place some high vacuum grease supplied with the system around the transduc er pin in the myograph chamber Make sure that the hole is completely sealed so that absolutely no buffer solution is able to enter the transducer compartment and damage the force transducer NO Figure 4 2 The Allen screws to be loos ened when replacing the transducer Important The new force transducer must be weight calibrated prior to running an experiment 4 3 Myograph maintenance The Muscle Strip Myograph System Model 800MS is a delicate and sophisticated piece of research equipment DMT recommend that the following sections are read carefully and that the instructions are followed at all times 4 3 1 Myograph chamber pipes To prevent the pipes from being blocked by buffer salt deposits after an experiment remove the chamber cover from the myograph and turn on the vacuum pump and User manual 800MS Chapter 4 23 vacuum valve for about 10 seconds continuously press the valve button down Turn off the vacuum pump and then the oxygen supply Remove any buffer remaining on the outside of the pipes with absorbent paper 4 3 2 Force transducer The force transducer is the most delicate and fragile component of the myograph system and it should therefor
24. hich can be determined by monitoring the interference while turning the equipment off and on the user is encouraged to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different to that which the receiver is connected to e Consult the dealer or an experienced radio TV technician for help Approvals Complies with the EMC standards EMC 89 336 EEC EN 61326 2 6 2005 EN 61000 3 2 Certified with the safety standards Directive 2006 95 EC EN 61010 1 2001 EN 61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 EN 61010 2 101 2003 DMT Muscle strip myograph system Model 800MS Certificate of Conformity DMT A S Skejbyparken 152 8200 Aarhus N Denmark hereby declares its responsibility that the following product Muscle Strip Myograph System Model 800MS version 2 2 is covered by this certificate and marked with CE label conforms with the following standards EN 61010 1 2001 Safety requirements for electrical equipment for EN61010 1 Corr 1 2003 measurement control and laboratory use EN 61010 1 Corr 1 2003 Part 1 General requirements EN 61010 2 101 2003 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment EN 61326 2 6 200
25. l weight calibration However in order to ensure that the myograph is working at highest performance DMT recommends that a new weight calibration is performed before starting to use the myograph system The weight calibration procedure is described in detail in Chapter 4 User manual 800MS Chapter 2 15 Chapter 3 The Myo Interface 3 1 Turning on the Myo Interface When the 800MS Myo Interface is switched on the start up Muscle Strip Myograph System Model 800MS message depicted to the right is shown Version 2 2 After a few seconds during which the system autocalibrates the A D converters the force menu will be displayed automatically 3 2 Main menus and submenus General navigation The various menus are selected the data changed and values entered with the F1 F2 and the arrow buttons The F1 and F2 button functions differ according to the current menu while the arrow buttons typically have the following functions Increase and decrease the data in the active line respectively 1 Scroll up and down in the menus The active line in the menu is indicated by a symbol displayed in the left side of the display Force menu The force menu provides an on line reading of the force values in BT ale millinewtons mN Bonos Force 3 Force 4 Removing and replacing a chamber unit When a chamber unit is unplugged from the Myo Interface the force display does not show a force value An example
26. ld be no force applied to the transducers when zeroing Heat menu In this menu the heating and temperature settings are con trolled The F1 button turns the heat ON and F2 switches it ip E OFF The temperature the myograph heats to Set temp PANES s is decreased with the button and increased with Act Temp is the actual temperature measured by the external temperature probe when connected to the interface If there is no probe connected Act Temp OFF will be displayed Note Whenever the system is turned on by the power switch on the rear panel the heating is automatically off until turned on by the user When the heating is on the set temperature will be reached in 20 minutes Recorder menu calibrated outputs The 800MS Myo Interface has four 1 V full scale analog out gt Force 1 OUT puts which provide the force signal in volts for connection to Force 2 OUT DNO Force 3 OUT an external data acquisition system By default the interface is Force 4 OUT set at 20 mN where 20 mN corresponds to 1 V As the force value will vary according to the maximum response from the preparation used the maximum force value can be changed by firstly making the channel of interest active and then increas ing or decreasing the value using the or buttons Irre spective of which maximum force value is entered the analog output is fixed to 1 V at max Calibration menu The calibration menu permits c
27. m defects in materials and workmanship for a period of three years after the date of delivery DMT A S will repair or replace any defective part subject to the conditions limitations and exclusions Exclusions Force and pressure transducers separately or part of myograph systems manufac tured by DMT A S are disclaimed from any warranty Limitations This warranty shall not apply to equipment subjected to accidental damage improper use alteration or deterioration Warranty on third party products will be as determined by their respective manufac turer DMT A S shall not be liable for consequential incidental special or other direct or indirect damages resulting from economic loss or property damage sustained by you or any end user from the use of the products sold or services rendered hereunder Warranty returns A Return Material Authorisation RMA number is required for all returns This number should be clearly indicated on all returned myograph systems Products damaged due to improper or inadequate packaging when returned for RMA purposes are not granted warranty coverage DMT Muscle strip myograph system Model 800MS Appendix 2 Service check A myograph working at optimal performance is extremely important for success when studying isometric force recordings To make sure that our customers always are deal ing with first class myographs DMT offers a Myograph Service Check at a very favor able price The Myo
28. o ensure longevity the needle valves should be turned regularly complete ly in and out a few times and greased to prevent them from becoming stiff use the linear grease provided with the system 3 Suction connection The system has an inbuilt aeration manifold with separate valves that allow each chamber to be drained individually After connecting your suction source to the interface the appropriate chamber will be drained by pressing the associated numeric button Note When draining the chambers using the automatic suction function continu ing to press the button for an additional 3 5 seconds after the chamber appears to be empty will ensure no residual solution is retained in the tubing and valves 4 Chamber covers The chamber covers are used to keep the temperature and other conditions gas tension pH of the solution surrounding the mounted segment as accurate as possible fig 2 3 Optional stimulation electrodes are available built into the chamber cover and can be used to activate the muscle via field stimulation fig 2 4 gt AAA e Oxygen supply ms OI e Suction connection Figure 2 2 Oxygen supply and suction connection 14 DMT Muscle strip myograph system Model 800MS Figure 2 3 Chamber cover Figure 2 4 Chamber cover with in built electrodes optional 2 3 The first weight calibration Prior to the shipment of the Muscle Strip Myograph 800MS it has gone through two days of continuous testing including a fina
29. ointed out in fig 4 1 The force applied on the force transducer should mimic the stretch created by the contrac tion of the mounted preparation Wait until the relative force reading is stable Press F2 to finish the calibration User manual 800MS Chapter 4 22 The 2 g weight is placed on this pan The tip of the transducer arm on the balance is positioned behind the clamp support Figure 4 1 Weight calibration setup T Force transducer 1 is now weight calibrated to an output at 9 81 mN The display will automatically return to the Force menu Note If the force reading is unstable or 20 1 mN different from 9 81 mN repeat the weight calibration If the instability continues refer to section 4 2 Repeat the calibration procedure for units 2 3 and 4 After calibrating all the myograph transducers carefully remove weight balance and calibration bridge and store The myograph system is now ready for use 4 2 Checking or replacing force transducer 4 2 1 Simple transducer check The myograph force transducer is a strain gauge connected in a Wheatstone bridge The force transducer for each myograph unit is located in a separate compartment While this provides some mechanical protection the force transducers are still very vulnerable to applied forces exceeding 1 newton 100 g or fluid running into the transducer compartment due to insufficient greasing of the transducer pinhole If the force reading continues to be unst
30. or a few minutes to dissolve calcium deposits and other salt build up Use a swab stick to mechanically clean all chamber surfaces 2 Remove the acetic acid and wash the myograph chamber and supports several times with double distilled water 3 If any kind of hydrophobic reagent have been used which might be difficult to remove using step 1 and 2 then try incubating the chamber and supports with 96 ethanol or a weak detergent solution 4 To remove more resistant or toxic chemicals incubate the myograph chamber and supports with 1M HCI for up to 1 hour In exceptional cases incubate the chamber and supports with an up to 3M HNO solution for about 15 minutes 5 Wash the myograph chamber and supports several times with double distilled water Important Notes Be very careful using step 3 and 4 repeatedly times as strong reagents may cause extreme damage to the myograph unit After cleaning ALWAYS check that the greasing around the transducer pin is suf ficient to keep the buffer solution away from the transducer compartment In cases of red or brown discolorations appearing on the chamber sides or on the supports the following cleaning procedure will work in most cases 1 Incubate the myograph chamber and supports for 30 minutes with 20 uL of a 2mM T 1210 Tetrakis 2 pyridylmethyl ethylenediamine solution dissolved in double distilled water 2 Use a swab stick to mechanically clean all the affected surfaces during the l
31. r G 19745 8 cm straight trabeculum Pipettes DMT recommends CappAero upipettes Light source DMT recommends Schott Cold Light Source either Model KL 200 or Model KL 1500 LCD Water bath including heater DMT recommends Julabo 5 L open bath circulator with plexiglass bath tank Glass bottle 2 L Vacuum pump DMT recommends a membrane vacuum pump with a volume of at least 6 L min Dissection petri dish DMT Item PD 2000 DMT recommends a 9 cm glass Petri dish coated with a 5mm Sylgard polymer layer A4 2 Myograph 800MS system accessories This section contains a list of special accessories available for the Muscle Strip Myo graph System Model 800MS ADI PowerLab data acquisition system Including Chart data acquisition and analysis software User manual 800MS Appendix 4 31 32 pH meter Including pH electrode and pH meter Standard PC system DMT Item 80150 DMT CS 200 4 Channel Current Stimulators Combined pulse and train generator Vertical hooks another possibility as supports A4 3 Myograph 800MS system spare parts This section contains a complete list of standard spare parts available for the myo graph 800MS For parts not listed in this appendix or for special parts which may need to be custom made please contact DMT for further information Force transducer DMT Item FT 61780 High vacuum grease and grease for screw thread DMT Item HVG 1000 Calibration kit DMT Item CK
32. ter and the other end of the cable to the RS485 plug on the myograph system that will be 1 Connect a serial cable to the other RS485 plug on the inter face Other end of cable must be connected to system 2 Repeat this if 3 or 4 systems are linked together Important When using the serial connection the systems must always be linked as follows system 1 to 2 2 to 3 and 3 to 4 DMT Muscle strip myograph system Model 800MS When all systems are linked together in the above order enter submenu 10 Press to change serial port to RS485 Use F1 to move the active line marker gt down and select the system number equivalent to the way in which the serial cables have been connected Exit the serial port menu by pressing 1 User manual 800MS Chapter 3 19 20 Chapter 4 The muscle strip myograph unit Chapter 4 contains a complete explanation of how to calibrate and maintain the 800MS myograph units to ensure the equipment is always performing to the highest standard 4 1 Calibration of force transducer As a part of the general maintenance of the myograph DMT recommends that the myograph is weight calibrated at least once every month DMT also recommends that the myograph is weight calibrated every time the system has been moved or has not been used for a long period of time 4 1 1 Principles of weight calibration Weight calibrating the force transducer is based on simple physics the net torque acting on a balance
33. ulator e D il switch Gas Power connector Suction i H RS 485 Port for serial connection to PC 4 Recorder outputs calibratedje bna eRS 282 Port for serial connection to PC Temperature probe E User manual 800MS Chapter 1 11 1 3 Muscle strip myograph unit Connection to Myo Interface bd Micrometer Force transducer pin Myograph clamp support con i nected to micrometer Myograph clamp support connected DMT Muscle strip myograph system Model 800MS Chapter 2 Setting up 2 1 The complete muscle strip 800MS myograph system Myo Interface front panel Power supply PowerLab data acquisition system optional DMT CS200 Pulse Train Stimulator optional PC USB connection Myo Interface rear panel PC data acquisition and analysis software optional Y Power supply Connection to oxygen supply BNC cables Vacuum pump optional RS serial connection Suction bottle optional o en Optional Figure 2 1 The complete muscle strip myograph system Model 800MS 2 2 Setting up step by step The chapter contains a complete step by step description of how to set up a complete Myograph 800MS System as illustrated in fig 2 1 1 Myo Interface PC connection Data acquisition is possible either by connecting the Myo Interface directly to a PC or through a PowerLab data
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