Electroblotting Systems - Denville Scientific Inc.
Contents
1. Hinged 10 x 10 cm cassette with 2 fiber pads E4136 10 x 10 cm fiber pads pkg 4 E4137 Complete Electroblotting System for E4203 20 x 20 cm Standard Gels Replacement parts and accessories Hinged 20 x 20 cm cassette with 2 fiber pads E4236 20 x 20 cm fiber pads pkg 4 E4237 Warranty Denville warrants that the unit you have received has been thoroughly tested and meets its published specification This unit excluding all accessories is warranted against defective material and workmanship for a period of twelve 12 months from the date of shipment ex factory Denville will repair all defective equipment returned during the warranty period without charge provided the equipment has been used under normal laboratory conditions and in accordance with the operating limitations and maintenance procedures outlined in this instruction manual and when not having been subject to accident alteration misuse or abuse No liability is accepted for loss or damage arising from the incorrect use of this unit Denville s liability is limited to the repair or replacement of the unit or refund of the purchase price at Denville s option Denville is not liable for any consequential damages Denville reserves the right to alter the specification of its products without prior notice This will enable us to implement developments as soon as they arise Denville s products are for research use only A return authorization must be obtained from2. Package includes Cooled tank lid a black cable a red cable 4x electroblotting cassettes and 8x fiber pads System Details Usage guidance and restrictions Maximum altitude 2000 m e Temperature range between 4 C and 60 C Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Not for outdoor use This apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 664 POLLUTION DEGREE 2 states that Normally only non conductive pollution occurs Occasionally however a temporary conductivity caused by condensation must be expected e p3 e p4 Using the Denville Electroblotting Units A Attaching Electrode Leads to the Lid 1 Note the position of the lid on the unit This shows the correct polarity and the correct orientation of the cables black is negative and red is positive 2 Remove the lid from the unit Note if the lid is not removed fitting the cables may loosen the gold plug and cause damage to the electrode 3 Screw the cables into the tapped holes as fully as possible so that there is no gap between the lid and the leading edge of the cable fitting 4 Refit the lid B Care and Maintenance Units are best cleaned using warm water and a mild detergent Water at temperatures above 60 C can cause damage to the unit and components The units should not be left in detergents for more than 30 minutes The tank should be thorough
3. Denville before returning any product for warranty repair on a freight prepaid basis e p9 e p10 Disposal This equipment is marked with the crossed out wheeled bin symbol to indicate that it must not be disposed of with unsorted waste Instead it is your responsibility to dispose of your equipment correctly at lifecycle end by handing it over to an authorized facility for separate collection and recycling It is also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so that the persons involved in the disposal and recycling of the equipment are protected from any potential hazard For further information about where you can drop off your waste equipment please contact your local Denville representative By doing so you will help to conserve natural and environmental resources and you will ensure that your equipment is recycled in a manner that protects human health Thank you Denville Scientific Inc P O Box 4588 Metuchen NJ 08840 4588 Toll Free 1 800 453 0385 Phone 1 908 757 7577 Fax 1 908 757 7551 Email info densci com Web www densci com 2011 Denville Scientific Inc All rights reserved Printed in the USA DENVILLE SCIENTIFIC INC
4. eo manual Electroblotting Systems Complete Electroblotting System for 10 x 10 cm Mini Gels Cat No E4103 Complete Electroblotting System for 20 x 20 cm Standard Gels Cat No E4203 DENVILLE um DNTV100 EBK IM Rev A0 08 11 SCIENTIFIC INC Contents Safety Warnings and Precautions General Information Packing Lists System Details Using the Denville Electroblotting Units A Attaching Electrode Leads to the Lid B Care and Maintenance C Tank and Cassette Preparation D Final Assembly and Transfer Ordering Information Warranty Disposal O Oo ON DU BP BUN S e pii Safety Warnings and Precautions Important user information Please read this entire manual to fully understand the safe and effective use of this product The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument Should you have any comments on this manual we will be pleased to receive them at Denville Scientific Inc P O Box 4588 Metuchen NJ 08840 4588 Denville Scientific Inc reserves the right to make changes in the specifications without prior notice When used correctly these units pose no health risk However these units can deliver dangerous levels of electricity and are to be operated only by qualified personnel following the guidelines laid out in this instruction manual The u
5. ly rinsed with warm water and distilled water to prevent build up of salts but care should be taken not to damage the electrode Vigorous cleaning is not necessary or advised Air drying is recommended before use Caution The units should never come into contact with the following cleaning agents these will cause irreversible and cumulative damage Acetone Phenol Chloroform Carbon tetrachloride Methanol Ethanol Isopropyl alcohol Alkalis Caution DEPC is a suspected carcinogen Always wear gloves and safety glasses e p5 Note Use gloves when handling the compression cassette components e p6 C Tank and Cassette Preparation 1 10 12 Prepare the appropriate amount of transfer buffer see General Information page 1 Fill the tank halfway with transfer buffer Connect the cooling water supply to the tank base hose connectors Use hose clips to secure the tubing Add a magnetic stir bar to the tank to maintain uniform temperature and conductivity during electrophoretic transfer Pre wet nitrocellulose or nylon membranes with distilled water Pre wet PVDF membranes in methanol Then soak all membrane types in transfer buffer Familiarize yourself with the interlocking hinge of the compression cassette Place the open cassette in a tray filled with transfer buffer so that molecules will migrate toward the membrane see Figure 1 For negatively charged macromolecules nucleic acids and most proteins b
6. nit must never be used without the safety lid correctly in position The unit should not be used if there is any sign of damage to the external tank or lid These units comply with the statutory CE safety directives 73 23 EEC low voltage directive IEC 1010 1 1990 plus amendment 1 1992 EN 61010 1 1993 BS EN 61010 1 1993 General Information The Denville Electroblotting Systems provide a cost effective safe and easy to use system for the transfer of proteins and nucleic acids from 10 x 10 cm mini and 20 x 20 cm standard gels Both units are supplied with four asymmetric color coded cassettes as a unique design feature to prevent them from being inserted into the tank in the wrong orientation against the direction of transfer Operational Model Buffer Volume Maximum Voltage E4103 2L 100 V E4203 6 3L 200 V e pi ep Packing Lists The packing lists should be referred to as soon as the unit is received to ensure that all components have been included The unit should be checked for damage when received Be sure to keep all packaging material for damage claims for to use should it become necessary to return the unit Please contact your supplier if there are any problems or missing items Complete Electroblotting System for 10 x 10 cm Mini Gels Package includes Cooled tank lid a black cable a red cable 4x electroblotting cassettes and 8x fiber pads Complete Electroblotting System for 20 x 20 cm Standard Gels
7. uild the transfer stack on the red half of the cassette Place a fiber pad on the red half of the transfer cassette and press until all air is expelled Place a sheet of blotting paper on the fiber pad and then place the membrane on the blotting paper Place the gel which contains a sample that has been electrophoretically separated and equilibrated if required with transfer buffer on the membrane Use a clean glass rod soaked in transfer buffer to roll out any trapped bubbles between the membrane and gel Cover the gel with a sheet of blotting paper and then a second fiber pad again pressing to gently expel any trapped air Close the cassette and slide the clamping clip into place Figure 1 Cassette Assembly Compression cassette Compression cassette Fiber pad Fiber pad Blotting paper Blotting paper Gel Membrane Direction of transfer D Final Assembly and Transfer 1 Insert the cassette into one of the tank slots with the transfer membrane facing the positive side of the tank Repeat for all cassettes being used for transfer Add additional transfer buffer to the tank to reach the max fill line Place the lid onto the tank and connect the leads to a power supply Set the voltage and current to suit the transfer application ep e ps Ordering Information Product Cat No Complete Electroblotting System for E4103 10 x 10 cm Mini Gels Replacement parts and accessories
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