Home

Eppendorf Xplorer® - Operating manual

1. Xplorer Additional volumes during aspiration Single channel and Ads Dis Reverse Pipetting multi channel 10 ul approx 0 6 uL approx 2 uL 100 ul approx 7 uL approx 20 uL 300 ul approx 20 uL approx 60 uL 1 000 UL approx 65 uL approx 200 uL 1 200 UL approx 140 uL approx 220 uL 5mL approx 0 3 mL approx 1 mL 10 mL approx 0 55 mL approx 1 8 mL only available as 8 channel The counter provides information on the performed dispensing operations in the Pip mode You can switch the counter on and off with the rocker After you select the Pip mode an activated counter shows You can change the value in the Pip mode When OM is activated the piston moves to the basic position when a filled pipette tip is ejected CAUTION Unintended dispensing when the Ejector reset option is activated When working with a removed ejector sleeve set the Ejector reset option to OFF to prevent unintended dispensing from the pipette tip in case the ejector is accidentally pressed 5 12 5 Sound level 5 12 6 Brightness You can change the sound volume of the acoustic signals If the volume has been set to the tone is switched off and no loudspeaker symbol appears in the header of the display The display brightness can be adjusted to meet your requirements Eppendorf Xplorer amp Operating manual 5 Operation 5 12 7 Adjustment d Changing the adjustment will cha
2. Operating manual 5 Operation 5 9 Pipetting The Pip mode is used to aspirate a liquid and to dispense it again The blovv out can be performed manually or automatically vvith the dispensing step 5 9 1 Standard pipetting VIE Aspiration Dispensing Blow out d 1 Prepare the Xplorer see Preparing the Xplorer on p 20 2 Press the rocker up to aspirate liquid 3 Press the rocker down to dispense the liquid Keep the rocker pressed down during dispensing if dispensing and the blow out blow are to be perfumed in a single step Executing the blow out blow immediately allows aqueous solutions to be processed faster If you keep the rocker pressed down after dispensing and blow out blow has been completed the piston remains in the lowest position The piston only moves to its basic position when you release the rocker As a result liquid flowing out at a slow rate can flow out completely Or if immersed into the liquid of the destination tube this liquid is not aspirated by accident 4 f the piston is in the basic position you can trigger the blow out blow at any time by pressing the rocker down If you keep the rocker pressed during the blow out blow the piston moves to the lowest position and remains there until the rocker is released It is possible to stop the aspiration or dispensing of liquids at any time by pressing the rocker in 0 the other direction The display shows
3. Mu ltkehannel 11 9 7 9 2 2 Delivery package 1 1 1 41 1 1 5001 10 2 3 NN INT 1 1 0 10 24 Waranty or 1 7 7 7 877 6 11111 1 1 1 1 10 20 IDISPIAVE sas cr REL 77 7 Ii be ILL RAE 11 2 6 MatorlalS i atu dha r r t mo 13 3 9 7 7 EB BDoRoaoeE HHHH HHHH ode XE 14 3 1 Intended HIE 3 2 12 gt 7 gt 3317 980 0 14 3 2 Warnings for intended use aaa ce ee tinia hesia b n 14 3 3 Information on product liability 34 15 4 Installation dd es deg 11 111 117 ewe wae ba ee EE EIN 16 4 1 Power supply assembly ass 111 eee 16 4 2 Inserting the Rechargeable lithium polymer baten 16 4 3 Charge the rechargeable baten 17 WE ei LEE 19 5 1 Principle of operation 2 44 9o a balk em A nea ar nek headed e n oS dinan dada pea RO Sba eya ue 19 5 2 Operating lil Od EE 19 5 3 Switching the pipette on and oft 19 5 4 JPreparing the Xplorer cn s sx sae iodo 20 5 4 1 Selecting the operating mode a
4. Turn the selection dial until the arrow points to Opt Press the rocker up or down to mark a parameter If required press the Help softkey to read information on the marked parameter Press the Select softkey to open the marked parameter e N gt Press the rocker up or down to change the parameter or to select a parameter in the new lower level 6 When the sublevel is opened press the Select softkey and follow the user prompts shown in the display 7 Press the End or Back softkey until the display with the name Option appears again in the status line second field from the top 8 Turn the selection dial to an operating mode to continue with dispensing 35 0 40 Maintenance 36 Eppendorf Xplorer amp Operating manual 6 Maintenance All single and multi channel lovver parts are wear parts Clean them after contamination use of aggressive chemicals and or heavy stress If the lower parts are worn or damaged replace the respective parts Faulty dispensing results are sometimes due to lack of maintenance 6 1 Cleaning gt Damage to device from unsuitable cleaning fluids or sharp or pointed objects Unsuitable cleaning fluids can damage the display surfaces and printing Never use corrosive cleaning fluids strong solvents or abrasive polishes NOTICE Check the compatibility with the materials used Do not use acetone to clean the Xplorer pipette
5. ep EE ee ce ce C C Ce 22339 Te C e C e C ces Hamburg e C TES C c c c ce c ermany c e f EE U Ee d G c 1 4 C qe Q c e e te E Gett ce C TA ce i e a Te LORS e C e LE GA e C c ge C eS c e C c E c Ce Eppendorf offices AUSTRALIA amp NEW ZEALAND AUSTRIA BRAZIL Eppendorf South Pacific Pty Ltd Phone 61 2 9889 5000 Fax 61 2 9889 5111 E mail Info eppendorf com au Internet www eppendorf com au Eppendorf Austria GmbH Phone 43 0 1 890 13 64 0 Fax 43 0 1 890 13 64 20 E mail office eppendorf at Internet www eppendorf at Eppendorf do Brasil Ltda Phone 55 11 30 95 93 44 Fax 55 11 30 95 93 40 E mail eppendorf eppendorf com br Internet www eppendorf com br CANADA CHINA CZECH REP amp SLOVAKIA Eppendorf Canada Ltd Phone 1 905 826 5525 Fax 41 905 826 5424 E mail canada eppendorf com Internet www eppendorfna com Eppendorf China Ltd Phone 86 21 38560500 Fax 86 21 38560555 E mail market info eppendorf cn Internet www eppendorf cn Eppendorf Czech amp Slovakia s r o Phone 420 323 605 454 Fax 420 323 605 454 E mail eppendorf eppendorf cz Internet www eppendorf cz www eppendorf sk FRANCE GERMANY INDIA Eppendorf France S A R L Phone 33 1 30 15 67 40 Fax 33 1
6. Do not use sharp objects to clean the Xplorer pipette Remove any contamination on the outside of the Xplorer pipette as follows gt Wet a cloth with a mild cleaning fluid and water and remove the contamination Toremove heavy contamination resulting from liquid penetration disassemble the lower part of the pipette see Xplorer pipette disassembly and assembly on p 38 and clean it with demineralized water Relubricate the piston sealing rings after contamination use of aggressive chemicals and or heavy stress Remove the old grease before relubricating 6 2 Sterilizing or disinfecting the pipette Damage to device from incorrect handling Only autoclave the lower part of the Xplorer pipette NOTICE Do not use any additional disinfectants decontamination agents or sodium hypochlorite when autoclaving the lower part When autoclaving the lower part make sure that the temperature does not exceed 121 C Before using disinfecting agents or decontaminating agents test for material compatibility and check the manufacturer s instructions about chemical resistance Consider also the material of the pipette 6 2 1 Autoclaving All Xplorer lower parts are steam autoclavable Before autoclaving 1 Remove any contamination from the exterior and in the lower part see Xplorer pipette disassembly and assembly on p 36 2 If you remove grease only relubricate the piston seal slightly with the grease specified in the o
7. Eppendorf Xplorer Operating manual eppendorf Copyright 2009 Eppendorf AG Hamburg No part of this publication may be reproduced without the prior permission of the copyright owner eppendorf epT I P S 9 and Eppendorf Xplorer are registered trademarks of Eppendorf AG Hamburg Germany Trademarks are not marked in all cases with TM or in this manual Xplorer pipettes are using U S Patents No 6 778 917 7 434 484 and U S Patent Applications No 200710276546 A1 200810006100 A1 200810011042 A1 200810034898 A1 Eppendorf Xplorer Operating Manual 01 0610 Eppendorf Xplorer amp Operating manual Table of contents 1 User instructions aset KSE kas la als c ki a lk ka ir aka a a aa ala EROR RU 6 5 1 1 Using this mandler 22322 de dri u LN at hie ed hee 11 headed eee 5 12 Warning signs and hazard icons kk kk kk kk kk kk kk KK kK m m 5 W221 iHazardcorns obese ee be oe icem 5 1 2 2 Dangerlevels 1111 S 5 1 3 SSYMDOISTU S OI 3 kd del ted al Eel eb de Pete dla de ed ed y k dw deh be ER ES 5 1 4 le NY E 6 2 Product description ei s ss kk sas sala i alla j aa ki ak al akla E a 8 2 1 Main lustration EE 8 21 11 Single chantiell 22 de 27 7 939 9 9090 0 7 7 311 11 9 8 25152
8. 1 User instructions 1 4 Glossary d Additional volumes Air cushion principle Biopur Free jet dispensing Increment ISO 8655 Nominal volume PCR clean PhysioCare Concept amp A The total of the remaining stroke and the reverse stroke An air cushion is located between the piston cylinder system of the pipette and the liquid in the pipette tip Upward movement of the piston produces a partial vacuum causing the liquid to be drawn into the pipette tip During the downward movement of the piston the air cushion is pressed downwards and the liquid in the pipette tip is dispensed Piston stroke optimization measures ensure that the stroke volume results in the liquid volume shown in the display Pipettes in which the piston is brought into contact with the liquid to be dispensed are defined as positive displacement pipettes B Eppendorf Biopur is an Eppendorf AG quality and purity standard for disposables In addition to the requirements for standard goods e g precision accuracy wetting behavior and tightness Biopur goods also meet the requirements for sterility absence of ATP human and bacterial DNA pyrogen and RNase Inspection and certification of Biopur goods is conducted by an external laboratory Batch specific Biopur certificates can be downloaded from our website at www eppendorf com F Dispensing steps which do not contact the wall of the tube If a drip forms on the pipette tip after
9. free jet dispensing this drip always belongs to the next dispensing step We recommend completing a dispensing series using only the free jet method or wall dispensing method Complete the reverse stroke in accordance with the dispensing series using either the free jet or wall dispensing method The measurement deviations listed in the technical data were determined using the wall dispensing method Step size or resolution For example with the volume selection on a 10 mL Xplorer pipette the volume can be increased or decreased in 0 01 mL steps The ISO 8655 standard defines among other things limiting values systematic error accuracy and random error precision and the testing procedures for dispensing devices N The maximum dispensing volume of a pipette The nominal volume is also used as the name of a pipette The term nominal volume comes from the ISO 8655 standard P PCR clean is an Eppendorf AG purity standard for disposables Products labeled with PCRclean are certified free of human DNA DNase RNase and PCR inhibitors A batch specific certificate can be downloaded from our homepage www eppendorf com Products that carry the Eppendorf PhysioCare Concept logo feature optimized ergonomics and make operation as simple as possible even with complex dispensing tasks PhysioCare products can be easily serviced and cleaned They are made of extremely light materials and require minimal operating force Eppendorf Xplor
10. s In older literature the unit P or cP is frequently found 1 mPa s corresponds to 1 cP At room temperature a 50 glycerine solution has a viscosity of approx 6 mPa s As the glycerine concentration increases viscosity increases considerably Absolutely anhydrous glycerine has a viscosity of approx 1480 mPa s at room temperature W Dispensing from a pipette while touching the tube wall with the pipette tip We recommend completing a dispensing series using only the free jet method or wall dispensing method suononaJjsur Product description Eppendorf Xplorer amp Operating manual 2 Product description 2 1 Main Illustration 2 1 1 Single channel 1 Connector socket For connecting the charging plug of an Eppendorf Xplorer power supply 2 Hardware reset button Situated at the center of the selection dial Trigger the hardware reset with a sharp object 3 Selection dial For setting an operating mode 4 Backlit color display The backlit color display simultaneously shows the selected mode and parameter settings such as the volume aspiration and dispensing speed and provides feedback and information on the work steps as well as further information 5 Softkeys The assignment of the respective softkey is shown in the footer in the display 6 Rocker You can either press the rocker up or down The rocker can either be used to trigger the piston movement or to scroll through the m
11. 2 2 2 2 2 2 2 2 6 1 6 1 6 1 6 1 6 7 1 2 1 2 1 2 1 2 8 0 85 0 87 0 85 1 0 53 ejep jeoiuuaal 11 Ordering Information 54 11 Ordering Information Eppendorf Xplorer amp Operating manual f CD The ordering information can be found on the CD for the Xplorer pipette Eppendorf Xplorer amp Operating manual A AQJUSHMON EE 45 7 34 2 point adjustment 7 34 3 point adjustment eese 34 A HIL Q ao siya pete ie ed eere dee Cebu d 34 epTIPS long 1 333 313 33 Ethanol Ze b y ka nek b b b n k ide 33 Factory settings eese 33 Glycerol Oe 33 Assemble OAO X 37 Assembly 10 uL 100 uL and 300 pL multi channel lower part 42 1200 uL multi channel lower pari 43 5 mL and 10 mL single channel lower part 40 Single channel lower part up to 1 000 pL 39 Auto dispensing eese 25 C mL 36 D Decoritaminatilori s ic la nan Mena as ar seo eee ache ne 49 Disassemble EE 37 Disassembiy 10 uL 100 uL and 300 pL multi channel lower part 41 1200 uL multi channel lower pari 42 5 mL and 10 mL single channel lower part 40 Single channel lower part up to 1 000 pL 38 D
12. 5 Maintenance 2 7 7 1 7 111 44 General information on adiustment 45 Troubleshootlng a s sada akku bx Rn ka hound uk Rm EEN EER ed EELER ER ENEE RR 47 8 1 Hardware 16 1111 11 47 8 2 Error Search 3 111 1111 47 Transport storage and disposal l l kk kak kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk kk 49 9 1 Decontamination before shipment kk kk kk KK KK KK KK KK hm mm 49 ENEE 0 0 EE EE 49 9 3 DISPOSAll mc 49 Technical data i c sa egenen a tad ee eee ECKE GORGE EUR CUR 1 090 0 50 10 1 Eppendorf Xplorer single channel kk kk kk kk kk KK KK KK KK KK KK KK KK KK KK KK mmu 50 10 2 Eppendorf Xplorer multi channel kk kk kk kk kK KK KK KK kK KK KK KK KK KK KK KK KK KK kK kK kK kK kK k 51 10 3 Speed table for Xplorer single channel kk kk kk kk KK KK KK KK KI KK KK KK KK KK KK KK KK KK KK KK 53 10 4 Speed table for Xplorer multi channel kk kk kk kk KK KK KK KK KK KK KK KK KK mm 53 Ordering Information 54 De ENEE 55 Eppendorf Xplorer amp Operating manual 1 User instructions 11 this manual gt Read this operating manual completely before using the device for the first time gt Consider this operating manual as part of the product and
13. If the pipette is used at an extremely high location it must be adjusted to the ambient air pressure see Adjustment on p 33 You can find an SOP Standard Operation Procedure for the test on our website www eppendorf com or on the Xplorer CD Eppendorf Xplorer amp Operating manual 9 Transport storage and disposal 9 1 Decontamination before shipment Decontaminate the pipette before shipping it to the authorized Technical Service for repairs or to your authorized dealer for disposal Please note the following A Risk to health from contaminated device A 1 Follow the instructions in the decontamination certificate It is available in PDF format on our homepage www eppendorf com decontamination and on the enclosed CD WARNING 2 Decontaminate all the parts you want to dispatch 3 Enclose the fully completed decontamination certificate for returned goods including the serial number of the device with the dispatch 9 2 Storage Damage to device due to inserted rechargeable battery during storage Remove the rechargeable battery from the Xplorer pipette if you do not intended to use the Xplorer pipette for an extended period NOTICE Air temperature Rel humidity Air pressure In transport packaging 25 to 55 C 10 to 95 70 to 106 kPa Without transport 5 to 45 C 10 to 95 70 to 106 kPa packaging 9 3 Disposal 3 In case
14. blue dark green 1 250 uL long 103 mm 50 1 200 uL 1 200 uL green dark green 1 250 uL long 103 mm 50 1 200 uL 5 mL purple not available 0 25 5mL 10 mL turquoise turquoise 10 mL long 243 mm 0 5 10mL The epT I P S specified here are also available as epDualfilter T I P S Altitude At increased altitudes where there is a fall in air pressure the aspiration volume of a piston stroke pipette is reduced The average air pressure at the corresponding height is used to correct the stroke The altitude can be selected in 250 m 820 ft steps The maximum altitude that can be selected is 5 000 m The Xplorer pipette is set to an altitude of 0 m on delivery This is the factory setting The liquid type Ethanol 7596 or Glycerol 5096 epTIPS long and Altitude options can be 0 combined For the following adjustments you must know the exact density of the solution to be dispensed The recording of the weighing results requires the use of a fine balance with a high resolution Dispensing operations under 10 pL require a balance with a resolution of 0 001 mg An existing 1 3 point adjustment can be changed 1 point adjustment After you enter the density the selected volume and the corresponding weighing results the Xplorer pipette will determine a correction factor The factor is only correct for the selected volume and the selected work technique However the factor is used for the entire volume range of the Xplorer pip
15. damaged Replace the lower part or channel The O rings of the tip cones are damaged Replace the O rings only 100 uL 300 UL 1 200 uL multi channel Liquid flows unevenly into or The tip is damaged Use a new tip out of the tip The piston is soiled Clean the lower part The seal is soiled 5 mL and 10 mL sizes replace the The pipette is blocked protection filter The display shows a wrench The pipette has been adjusted for another Check whether the adjustment selected in the left of the header liquid in the Options is correct and change it if necessary 47 Bunoouso qnou Troubleshooting 48 Eppendorf Xplorer amp Operating manual 8 Troubleshooting Symptom Possible cause Solution No spring loading action of the tip cone when taking up pipette tips Spring loading action is blocked by a locking ring Remove the locking ring again Use a 5 mL 10 mL single channel pipette or a 1 200 uL multi channel pipette No remedy The tip cone of these sizes do not have spring loading action Battery state a Battery empty Confirm with OK Gonnect power supply immediately K The rechargeable battery is highly discharged Confirm the message with the Ok softkey Complete the dispensing operation as quickly as possible and charge the rechargeable battery for 3 hours You can continue dispensing with the power supply unit conn
16. data No adjustment changes are allowed unless you can answer all the questions with yes In all other cases the problems associated with the questions answered with no must be eliminated If the problem is remedied by exchanging a complete lower part or other parts that have an effect on the volume proper assembly must be verified by carrying out a gravimetric test Refer to the technical data for the systematic and random errors to be met If you want to use the Xplorer to perform a 1 3 point adjustment for a liquid other than water you must also refer to the points described above when determining the weighing results Note in particular that the density of the test liquid must be determined exactly at the test temperature The density is dependent on the temperature The use of an incorrect density will result in an incorrect volume calculation CD When determining the weighing results and the measurement deviations also refer to the instructions given in the document Standard instructions for pipettes on the Xplorer CD Eppendorf Xplorer amp Operating manual 8 Troubleshooting 8 1 Hardware reset This function resets the Xplorer pipette to its original state Your saved settings are not lost Press a pipette tip or another pointed object against the contact point in the center of the selection dial The display shows your personaliza
17. ep a D er volume Maximum aspiration and dispensing volume Edit like Set volume Mi 0 HL 0 changed prior to liquid Speed ve 1000UL Manual pipetting The set value appears after editing in the display next to Max Edit ep Help Eppendorf Xplorer amp Operating manual 5 Operation Display Parameter Available in Ads Dis Pip PIM Man Dispensing 4 dl Set volume step AL 123 u Set volume step Volume per dispensing step Can only be changed prior to liquid aspiration Aspiration speed Eight aspiration speed levels are available The higher the numerical value the higher the speed Can be changed during operation Aspiration speed is also automatically approximately used for the blow out blow Dispensing speed Eight dispensing speed levels are available The higher the numerical value the higher the speed Can be changed during operation Dispensing speed is also automatically approximately used for the blow out blow ep Set time Interval between the dispensing step 0 1 sto 10s Dispensing adi a Number of steps A Steps ep Humber of steps The available number of dispensing steps depends on the setting for Set volumefstep and is automatically set to the maximum number when Set volume step is edited The remaining dispensing steps are displayed during operation 2
18. filter tips whose aspiration volume is smaller than the nominal volume of the Xplorer pipette After setting a volume limitation a message will appear in the display when the volume limit has been reached when editing the Pip P M and Man operating modes In the Dis and Ads modes the number of possible dispensing steps is automatically limited ep Dualfilter T I P S Volume limit Ads Dis Reverse Pipetting 10 ul not required not required 100 ul not required not required 300 ul not required 260 uL 1 000 uL not required 950 uL 1 250 uL long not required 1130 uL 5mL 4 9 mL 4 6 mL 10 mL 9 75 mL 8 5 mL To increase the dispensing accuracy of these filter tips we recommend the use of the adjustment epTIPS long The specified volume limit applies to the changed adjustment We also recommend this volume limit for reverse pipetting when using the 5 mL and 10 mL epT I P S without filter When using filter tips of other manufacturers other volume limits may apply The volume limit is different depending on the tip geometry and the position of the filter in the tip The volume limit is dependent on the aspirated additional volumes of the Xplorer pipette With the Xplorer the individual nominal volumes result in different additional volumes 31 0 40 Operation 32 Eppendorf Xplorer amp Operating manual 5 Operation 5 12 3 Counter 5 12 4 Ejector reset
19. keep it in a place where it can be easily accessed gt Include this operating manual with the device when passing the device on to third parties In case of loss of the operating manual please request a replacement The latest version can be found on our website at www eppendorf com This operating manual applies to devices with software version 01 05 00 or higher Amore detailed operating manual adjustment instructions and further information can be found on the Xplorer CD or on our website at www eppendorf com 1 2 Warning signs and hazard icons D 1 2 1 Hazard icons Biohazard Explosion Electric shock Toxic substances Hazard point Material damage 1 2 2 Danger levels The danger level is part of a safety note and defines the potential consequences if the information contained therein is not observed DANGER Will lead to severe injuries or death WARNING May lead to severe injuries or death CAUTION May lead to light to moderate injuries NOTICE May lead to material damage 1 3 Symbols used Depiction Meaning gt You are requested to perform an action ik Perform these actions in the sequence described 2 List Text Terms and key names from the software e References useful information 0 Jas User instructions Eppendorf Xplorer amp Operating manual
20. may be impaired The liability for the function of the device passes to the operator if The device is not used in accordance with the operating manual The device is used outside of the range of application described in the preceding chapters The owner has made unauthorized modifications to the device 15 Kjajes Installation Eppendorf Xplorer amp Operating manual 4 Installation 4 1 Power supply assembly J 1 Power plug adapters a EU b United Kingdom c USA d Australia 2 Power supply 3 Charging plug Fitthe power plug adapter required for your power supply into the opening of the power supply In the event of any doubt which power plug adapter should be used for the power supply unit you should ask an electrician for advice 4 2 Inserting the Rechargeable lithium polymer battery 3 Insert the rechargeable lithium polymer battery as follows 1 Open the rechargeable battery compartment cover 2 Insert the rechargeable lithium polymer battery 3 Connectthe plug of the rechargeable lithium polymer battery to the connector socket 4 Close the rechargeable battery compartment cover Eppendorf Xplorer amp Operating manual 4 Installation 4 3 Charge the rechargeable battery AN VVARNINGI Use of incorrect povver supplies may result in fatal injuries and damage to the device Use of incorrect power supplies may result in overheatin
21. the cylinder and the spring out of the upper rail Before installation slide the piston into the cylinder Install the channels in reverse order 41 Maintenance 42 Eppendorf Xplorer amp Operating manual 6 Maintenance Assembling the 10 300 pL multi channel pipette 1 2 3 Fit the cover plate with the integrated ejector Push the latches upwards To mount insert the lower part into the upper part until it audibly engages Make sure that the magnet on the piston actuator in the lower part is coupled with the magnet on the spindle in the upper part see p 43 Carry out a gravimetric test of the systematic and random error see p 50 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly Loosening and opening the 1 200 uL multi channel The method of loosening the 1 200 uL lower part matches that of loosening the 5 mL or 10 mL lower parts di 8 The piston must be in its basic position before loosening the lower part Press the rocker down to move the piston into its basic position With the ejector pressed down turn the lower part by approximately 30 and pull down to cancel the magnetic coupling This releases the lower part from the upper part View the detached lower part from above For this slide the housing case down Note the exact position of the red marked parts The red mar
22. the pipette moves down The liquid is aspirated into the pipette tip is dispensed Press the Edit softkey to open the edit mode of the selected operating mode You can change the parameter outlined and marked in red here with the rocker Special operating sequences with an electric pipette are possible with dispensing with the rocker For example you can abort a piston movement in the Dis Pip and P M modes at any time by pressing the rocker in the other direction In the Ads and Man modes you can abort the piston movement by releasing the pressed rocker After the piston has been stopped you can either continue the aspiration or the dispensing process by pressing the rocker in the corresponding direction If the piston is in the basic position you can repeat the blow out blow at any time by pressing the rocker down Controlling the piston movement according to how the rocker is actuated ensures that the piston only moves in the pressed direction 5 2 Operating modes You can set the desired operating mode using the selection dial The follovving operating modes are available Ads Automatic dispensing Dis Dispensing Pip Pipetting PIM Pipetting and Mixing Man Manual pipetting Opt Options 5 3 Svvitching the pipette on and off 3 d The Xplorer pipette automatically switches to standby mode after a certain period of inactivity Press any key to reactivate the display Switch on 0 10 1 Press any key
23. the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community Within the European Community the disposal of electrical devices is regulated by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE According to these regulations any devices supplied after August 13 2005 to the business to business area this product is assigned to may no longer be disposed of in municipal or domestic waste To document this they have been marked with the following symbol As disposal regulations may differ from country to country within the EU please contact your supplier if necessary X D A Risk of explosion and fire from overheated batteries AA gt Do not heat the battery above 80 C and do not throw it into the fire cC WARNING Disposal of batteries Do not dispose of batteries together with domestic waste Dispose of batteries in X accordance with local legal regulations 49 jesodsip pue 3Be40js 340dsueA J Eppendorf Xplorer amp Operating manual 10 Technical data W 10 1 Eppendorf Xplorer single channel Model Test tip Testing Eppendorf AG error limits Increment epT I P S color code Volume Error Av Volume tange Systematic Random Ed Length z jiL 96 pL E 0 5 10 uL medi
24. the volume present in the pipette tip after the respective operation has been stopped You can then continue with liquid aspiration or dispensing The number of dispensing steps performed can be counted with a counter Counter The counter can be activated Counter if desired see p 30 27 0 40 Operation 28 Eppendorf Xplorer amp Operating manual 5 Operation 5 9 2 Reverse pipetting d Reverse pipetting is useful for plasma serum and other liquids with a high protein content Reverse pipetting is not necessary when pipetting aqueous solutions Keep the rocker pressed down hl 4t E S E A Release the rocker Aspiration Dispensing Aspirate or empty Aspirate blow out With reverse pipetting more liquid is aspirated by using the blow out than with standard pipetting These additional volumes may result in volume limits when using filter tips see p 31 During dispensing the blow out blow does not belong to the dispensing volume Proceed as follows Keep the rocker pressed down Immerse the pipette tip into the liquid Release the rocker to aspirate the blow out Press the rocker up to aspirate the dispensing volume Place the pipette tip on the wall of the destination tube Press the rocker down to dispense the liquid Remove the pipette tip from the destination tube There is still residual liquid in the tip There are two courses o
25. to activate the pipette 2 Turn the selection dial so that the arrow points to an operating mode Turn the selection dial so that the arrow points to Off 19 0 40 Operation 20 Eppendorf Xplorer amp Operating manual 5 Operation 5 4 Preparing the Xplorer Before dispensing can commence you first need to select an operating mode set its dispensing parameters and attach a pipette tip s to the Xplorer pipette 5 4 1 Selecting the operating mode The arrow left next to the selection dial shows the current operating mode Set the desired operating mode using the selection dial 5 4 2 Set the parameter edit mode Change the parameters as follows Press the Edit softkey to open the edit mode Press the Mext softkey to go to the next dispensing parameter Change the dispensing parameter marked in the display with the rocker Press the End softkey to save the changes to exit the edit mode and to continue with dispensing SON ue If you want to cancel editing without saving the changes made briefly turn the selection dial to 0 another mode You can also open the edit mode during dispensing in order to change the dispensing parameters Speed Time Cycles and Counter Display Parameter Available in Ads Dis Pip P M Man Set volume e e Pipetting set volume A aspiration Aspiration and dispensing volume Can only be changed prior to liquid
26. to the lowest position and remains there until the rocker is released 5 12 Setting options In the Opt mode you can set the following options which are applicable to all operating modes Proceed as follows 1 Move the selection dial to Opt 2 Select the desired option with the rocker 3 Press the Select softkey to open the option Follow the user prompts in the display 4 Press the End or Back softkey to exit an option 5 Turn the selection dial to an operating mode to exit the options and to continue with dispensing operations If required you can press the Help softkey to access information on the marked parameter Eppendorf Xplorer amp Operating manual 5 Operation 5 12 1 General help 5 12 2 Volume limit This describes the elementary steps for all modes This option does not contain any adjustable parameters The help texts in the Xplorer are not a substitute for reading the operating manual This limits the aspiration volume in all dispensing modes Use the volume limit in the follovving cases For strongly foaming solutions in order to prevent contamination of the tip cone or the protection filter When using epDualfilter T I P S in the Ads Dis and Pip modes during reverse pipetting With these three versions there is the risk during aspiration that the liquid may come into contact with the Dualfilter because of the greater volumes for blow out and residual stroke For pipette tips or
27. with matching pipette tips In vivo applications applications in or on the human body are not permitted The Xplorer pipette may only be operated by trained specialist staff All users must have read the operating manual carefully and familiarized themselves with the device s mode of operation 3 2 Warnings for intended use Danger of explosion A gt Do not operate the device in areas where work is completed with explosive substances N jj DANGER Do not use this device to process any explosive or highly reactive substances Do not use this device to process any substances which could create an explosive atmosphere Damage to health due to handling infectious liquids and pathogenic germs N A Observe the national regulations for handling these substances the biological security level of your laboratory the material safety data sheets and the manufacturer s application notes WARNING Wear personal protective equipment PPE Follow the instructions regarding hygiene cleaning and decontamination For complete instructions regarding the handling of germs or biological material of risk group Il or higher please refer to the Laboratory Biosafety Manual source World Health Organization current edition of the Laboratory Biosafety Manual Damage to health due to toxic radioactive or aggressive chemicals gt Observe the national regulations for handling these subst
28. 0 0 2 Increment 2 200 uL 50 uL 1 0 0 5 0 8 0 4 la u 100 uL 0 8 0 8 0 25 0 25 15 300 uL orange 30 uL t2 5 0 75 t1 0 t 0 3 Increment 15 300 uL 150 uL 1 0 1 5 0 5 0 75 STE asm 300 uL 0 6 1 8 0 25 0 75 50 1 200 uL green 120 uL 6 0 7 2 0 9 1 08 only 8 channel 50 1 250 pL 600 uL 2 7 16 2 0 4 2 4 GER 1200uL 1 2 14 2 0 3 3 6 ejep 1 The errors specified apply only when the epT I P S pipette tips are used Test conditions and test evaluation in compliance with ISO 8655 Part 6 Test using a standardized fine balance with a moisture trap Check the volumes in the Pip mode keep the rocker pressed during the blow out blow Set speed level 5 Number of determinations 10 Use of water in accordance with ISO 3696 Test at 20 C 25 C 0 5 C Dispensing against the tube wall 51 Technical data 52 Eppendorf Xplorer Operating manual 10 Technical data Rechargeable battery Type Rechargeable Li polymer battery Capacity 750 mAh 3 7 V Charging time approx 3 hours Number of dispensing operations approx 3000 in the Pip mode with a fully charged rechargeable battery and medium dispensing speeds with a single channel version lt 1 000 LL Weight approx 20 g Power supply Type Power supply for Xplorer Multipette Repeater stream Xstream with
29. 1 0 40 Operation 22 Eppendorf Xplorer amp Operating manual 5 Operation Display Parameter Available in Ads Dis Pip PIM Man Pipetting Set counter A Gounter ep Set counter The counter counts the number of dispensing operations performed The numerical value of the counter is increased by 1 when the piston reaches the basic position after dispensing You can also change this value during operation by going to the edit mode with the rocker If the piston is in the basic position you can alternatively set the counter to 0 by turning the selection dial forward or backward You can switch the counter on and off in the Options 4 H Pipetting Mix Mixing volume A Mix 1000uUL ep Mixing volume The mixing volume used after pipetting If you change the pipetting volume under Set volume the mixing volume is also changed accordingly You can edit mixing volume again afterwards when the Mix line has been reached 4 m Pipetting Mix Mixing cycles A Cycles ep Mixing cycles 1 to 88 mixing cycles can be set for the mixing volume The mixing cycles are counted down to D If you keep the rocker pressed down during mixing after has been reached mixing is continued until the rocker is released If you press the rocker up during mixing the mixing process is stopped The mixing speed matches the selected sp
30. 12 The multi channel version has a piston for each channel so that less than 8 or 12 tips can also be mounted You can open the multi channel lower part in order to exchange or remove individual channels The 1 200 uL multi channel lower part is only available as a 8 channel version 3 Right and left latches Unlocks the cover plate 6 with interior ejector rail The 1 200 uL multi channel lower part can be opened with a pin see p 42 4 Spring loaded tip cones The spring loading action optimizes the force required for attaching and ejecting tips It is available for the volumes 10 uL 100 ul and 300 LL 5 Pipette tips It is recommended to use epT I P S 6 Cover plate for 10 uL 100 uL and 300 uL Detachable cover plate with an interior ejector rail The cover plate is opposite the side with the lever 1 uonduosap j3onpo4d Product description 10 Eppendorf Xplorer amp Operating manual 2 Product description 2 2 Delivery package Number Description 1 Eppendorf Xplorer pipette Rechargeable lithium polymer battery 1 Power supply with power plug adapters 1 Operating manual English German 1 CD Eppendorf Xplorer with extended operating manual in several languages ordering information and additional documents 1 Eppendorf Xplorer Short Instructions English Additional languages on the Eppendorf Xplorer CD 1 Locking ring for deactivating the spring loading action in the case of
31. 30 15 67 45 E mail eppendorf eppendort fr Internet www eppendorf fr Eppendorf Vertrieb Deutschland GmbH Phone 49 2232 418 0 Fax 49 2232 418 155 E mail vertrieb eppendorf de Internet www eppendorf de Eppendorf India Limited Phone 91 44 42 11 13 14 Fax 91 44 42 18 74 05 E mail info eppendorf co in Internet www eppendorf co in ITALY JAPAN NORDIC Eppendorf s r l Phone 390 2 55 404 1 Fax 390 2 58 013 438 E mail eppendorf eppendort it Internet www eppendorf it Eppendorf Co Lid Phone 81 3 5825 2363 Fax 81 3 5825 2365 E mail info eppendorf jp Internet www eppendorf jp Eppendorf Nordic Aps Phone 45 70 22 2970 Fax 45 45 76 7370 E mail nordic eppendorf dk Internet www eppendorf dk SOUTH amp SOUTHEAST ASIA SPAIN SWITZERLAND 3 Eppendorf Asia Pacific Sdn Bhd Phone 60 3 8023 2769 Fax 60 3 8023 3720 E mail eppendorf eppendorf com my Internet www eppendorf com my Eppendorf Ib rica S L U Phone 34 91 651 76 94 Fax 434 91 651 81 44 E mail iberica eppendorf es Internet www eppendorf es Vaudaux Eppendorf AG Phone 41 61 482 1414 Fax 41 61 482 1419 E mail vaudaux vaudaux ch Internet www eppendorf ch C UNITED KINGDOM USA OTHER COUNTRIES j Eppendorf UK Limited Phone 44 1223 200 440 Fax 444 1223 200 441 E mail sales eppendorf co uk Internet www eppendorf co uk Epp
32. ISINIECHON d si fate PRA eis i 36 DISPENSING EE 26 Ne 11 Disposal 33 0 49 E ide v rrr 20 Error limits Multi hanin c s vivo iiem eec 51 Single channel uk 50 F AN T ND 0 10 7 7 7 7 7 12 H Hardware 1 1 1 47 ET 11 L EEE ec 38 M Main field Ecc 12 Main Illustration multi channel inn ma ardene Ee i ee tee ient 9 Single Channel sese 8 Malintenanice 4 tE 44 Manual 1 30 0 Operating Mode EE 19 Manual pipetti nG aaa aaaaaa ana aaanaenen eee vonese vere re vene eee ene 30 a A E 27 Pipetting and mtsimg aaa nnna ene ne sene nes vee eee eee re 29 Reverse pipetting u keke 28 Standard keke 27 Option AGJUSUMENE EEN 33 Eet 32 all TEE 32 Ejector FOS aa mm em 32 General help u u 0 31 LANGUAQO 01 0 35 Personalization keke 35 SOOT VIGO ER 35 Sound 1 11 1 11 32 Volume limit ah aaa neneve nene nese eken k kek kk kk setet 31 P Pipeltin O 307 7 7 30 3 1 27 Pipetting and 00 keke 29 Power supply assembly sseseeeeee 16 R Rechargeable lithium polymer battery Ghatge 2 17 Inserting fe 16 Replace channel 10 uL up to 300 uL multi channel lower part 41 1 200 uL mu
33. Ie h Ee t grundlegend ce C ce C qe C carted nt the re ung t uns abgesti Anfora xs ce E e C 6 Produkt ed use th sid iet diese erunge 6 6 ce C bezei his de of Una ndame Erkl r A der a3 c ce C E ichnung EE ad ung 0 ce ce e Wi ce cE npend Product k of ltigkeit ce c e c orf Xpl name ri e C c c ce C oren product ce ge c ccc E C ee e K c EGE e C c 2 e C 6 ce C C ce 6 e C e C n EGE e ce c ge C C e C E c 6 ce C e C cc f SCH e C Mi C ee C e C E C e C C 6 C e C ce ce C TA GU reer C e C C e C E C e e C ce C Kon Kor e C t qe C T3 Ce e e e e C cc C E C e C ge C Vorsta e C ce e C c 17 Board of M ce C T3 6 c 9 2009 anageme c e C 6 c C SSES n e LE ce C ce C ce dog SC ce C C Wek GEG c ce C Projektma e LE 6 C C ef nagement e C ce ce C Pect Mana a KO ce C dos ec e E e c C YES e cet C ce C K ee ea t6 e C 6 015 ces e K e C qe C c press ces DP e C c o t ce C o C e Ce Ti Eppendor endo C cc c e ce C L ELE E U AG Barkh rf ce C 6 g C e C ce C Es
34. a 20 5 4 2 Set the parameter edit model 20 55 Using pibell EE 23 5 6 TIpsiorcorteci Pipen EES 23 bios 37 e do 1 Ee EE 23 5 6 2 Aspirating NGUT 226 2904 e hea hu t dhi gees s 24 5 6 3 Dispensing KUKA iz ad ineo in e EE t OU RI ME EY 24 5 7 Automatic dispernis Mo 1 EE 25 5 9 IDISPEMSHM ON sisse 3 3 RE es Bele IRR oe Be h e ev t ES 26 5 9 aeui 27 5 9 1 Standard pipetting nex n er RI cR beer AIR l a esta P d ee eve 27 5 9 2 Reverse pipetting 7 1 11111 21 1 9 28 5 10 Pib tlifig and MIXING 7 11 113 7 7 7 NE Wn VETE 29 5 11 11 30 5 12 Setting options a ee 30 5 12 1 Generalihelp lt 277 7 7 7 7 87 7 31 70 7 787 1 31 512 3 1 1 32 5512 4 EjectorTeset ves 777 7 1 n da W
35. after the completion of the language selection 0 When selecting a language the softkeys and the language selection list are always in English This makes it easier to return to the desired language should you select an unknown language by mistake 5 12 9 Personalization 5 12 10 Service You can use the rocker to select letters and numbers to personalize the pipette Your personal settings are displayed when you activate the pipette from sleep mode or after a hardware reset When the Xplorer pipette is delivered the personalization is My Kplorer The options listed here are not needed during normal routine use A selection list offers the following service options Software version Display of the software which is valid here Parameter version Display of the applicable data record for this Xplorer The data record is different for the various volumes nitial reset After a confirmation request appears all adjustment settings are reset to the factory settings and all entries are reset to the default values This type of reset may be useful when passing on the Xplorer pipette to another workstation Check procedure For service personnel only Not released for general use Software update For service personnel only Not released for general use If the IRDA does not receive a signal within 30 seconds the opened option is automatically closed IRDA For service personnel only Not released for general use
36. alling the locking ring For the following dispensing tasks it can be helpful to deactivate the spring loading action of the tip cone A pipette tip is to be used for a long period The pipette tip is slightly bended during dispensing Deactivating this action can also be helpful for pipette tips which are not designed for the Eppendorf pipette tip cone The spring loading action of the tip cone can be deactivated by installing the locking ring The locking ring is included in the delivery package 1 Push the black locking ring onto the lower part from the top Slightly squeeze the clamps on the lower part when doing so 2 Insert the lower part into the upper part until it engages audibly 3 Keep the ejector pressed The ejector rod protrudes from the upper part 4 Fitthe ejector sleeve onto the ejector rod It is fitted correctly if it engages slightly Remove the locking ring to reactivate the spring loading action Eppendorf Xplorer amp Operating manual 6 Maintenance Opening and assembling the lower part lt 1 000 pL To avoid confusion of parts only disassemble and assemble one pipette at a time 1 2 3 i 4 S PUSH UP TO RELEASE PUSH UP TO RELEASE PUSH UP TO RELEASE The piston must be in its basic position before separating the lower and upper part If necess
37. ances as well as the material safety 4 X data sheets and manufacturer s application notes WARNING gt Wear personal protective equipment PPE A Personal injury caused by the incorrect handling of the rechargeable battery d Donotdisassemble or modify the rechargeable battery WARNING Never pierce crush or throw the rechargeable battery Only use the rechargeable battery in the Xplorer pipette Do not touch leaky rechargeable batteries Do not use any damaged rechargeable batteries gt Dispose of damaged rechargeable batteries in accordance with legal regulations A Danger to persons from careless use A Never point the opening of a Xplorer pipette fitted with pipette tip at yourself or anyone else CAUTION Only initiate dispensing if it is safe to do so For all dispensing tasks make sure that you are not endangering yourself or anyone else A Poor safety due to incorrect accessories and spare parts Z The use of accessories and spare parts other than those recommended by Eppendorf may d b impair the safety functioning and precision of the device Eppendorf cannot be held liable or CAUTION accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment Only use accessories and original spare parts recommended by Eppendorf 14 Eppendorf Xplorer amp Operating manual NOTICE NOTICE Damage to dev
38. ary press the rocker down to move the piston to its basic position Opening the lower part 1 2 3 On the piston mount 1 push the locking mechanisms together slightly Remove the piston mount Remove the piston 2 and the piston spring 3 The piston is under spring tension Pistons and piston springs will look different for the individual volume versions Assembling the lower part 1 Carefully guide the piston and the piston spring into the cylinder Make sure that the piston is guided correctly in the piston spring and in the cylinder There must not be any perceptible resistance Stop pushing as Soon as any resistance is felt This may indicate that the piston is not positioned correctly in the cylinder There is a risk of bending the piston if too much force is applied Carefully pull out the piston and repeat the process correctly In the case of piston springs with double windings 4 these windings must point downwards Keep the piston and the piston spring pressed Keep the locking mechanisms on the piston mount pressed with the other hand Mount the piston mount in such a way that the two locking mechanisms engage in their retainers Gently press a pipette tip against the inserted piston The piston must move down in the cylinder without any noticeable resistance Installing the lower part and ejector sleeve Insert the lower part into the upper part until it engages audibly Set the maximum volume and op
39. as reached its basic position 4 After the mixing process press the rocker down to trigger the blow out blow If you keep the rocker pressed during the blow out the piston moves to the lowest position and remains there until the rocker is released 29 0 40 Operation 30 Eppendorf Xplorer amp Operating manual 5 Operation 5 11 Manual pipetting d i EA ES ES mcam E YI Aspiration Dispensing Blow out In the Man mode the Xplorer pipette is used like a manual pipette The maximum aspiration volume can be limited if required Aspiration can be stopped or continued or the direction changed as often as required The amount of liquid aspirated is limited to the volume selected in editing mode The volume selected in editing mode appears in the main field of the display to the right next to Max 1 Prepare the Xplorer pipette see Preparing the Xplorer on p 20 2 Keep the rocker pressed up or down to aspirate or dispense liquid The piston movement stops when you release the rocker The display shows the liquid volume in the pipette tip You can switch between liquid aspiration and dispensing as often as required 3 Ifthe piston is in the basic position you can trigger the blow out blow at any time by pressing the rocker down If you keep the rocker pressed during the blow out the piston moves
40. de red 32 5425 Sound leveli 1 0 32 0 JO ajqel Table of contents Eppendorf Xplorer amp Operating manual Table of contents 6 7 8 9 10 11 12 5 12 6 Brightness crasas penat 1 ETS RR tege AE d Na 32 542 7 Adjustment A Vs kont E EN x wd Eeer Aa ed aed deg e ee Dal eos E ee 33 EEN 35 5 12 9 Personalizatiori id qdo 97 7 7 7 7 7 7 21 35 51210 SENICE pepe 35 Malnt nance 71 36 6 1 Cleaning ea eos ne orte RUE Se aeg ROLE Reit d onde EE e 36 6 2 Sterilizing or disinfecting the pipette III III 36 6 2 4 Autoclavings 7 1 5 36 6 2 2 0 7 11 37 6 3 Replacing Osfiln08 1 33117 3539 37 6 3 1 Removing the O ring kk kk kk kk kk KK KK KK KK KK KK KK KI KK KK KK KK KK KK KK KK KK KK KK kk 37 6 3 2 Mounting a new O ring kk kk kk kk kk kK KK KK KK KK m mr 37 6 4 Xplorer pipette disassembly and assembly eee 38 6 4 1 Single channel uptoto0o0u KI KK RR Im 38 6 4 2 5 mL and 10 mL single channel kk kk kk kk kK kK kK kK KK KK KK KK KK KI KK KK KK KK KK KK kK kk e 40 6 4 3 M lti chan n l lt 1111 111 1 1111110 0 41 6
41. de whether the residual liquid should be dispensed or liquid should be aspirated again Liquid aspiration Press the rocker 1x up Liquid dispensing Press the rocker 2x down 25 0 40 Eppendorf Xplorer amp Operating manual 5 Operation 5 8 Dispensing Operation 26 ERB A 1 2 3 4 a ES ES JS Aspiration Reverse Dispensing Dispensing Dispensing Aspirate or stroke empiy The Dis mode is used to aspirate a liquid and dispense it individually in partial volumes of equal size The reverse stroke and remaining stroke are triggered before or after the dispensing steps These additional volumes may result in volume limits when using filter tips see p 31 1 Prepare the Xplorer pipette see Preparing the Xplorer on p 20 2 Press the rocker up to aspirate liquid To abort dispensing you can press the Empty softkey The tip is completely emptied 3 Press the rocker down to trigger the reverse stroke 4 Press the rocker down again for each dispensing operation The display shows the remaining dispensing steps under Steps After dispensing decide whether the residual liquid should be dispensed or liquid should be aspirated again An ongoing large dispensing volume operation can be stopped by pressing the rocker up Liquid aspiration Press the rocker 1x up gt Liquid dispensing Press the rocker 2x down Eppendorf Xplorer amp
42. e following 1 Upper part of the lower part Z 2 Piston with seal E Piston rod with magnet covered here by 1 Upper part of the lower part 3 Oylinder and tip cone The assembly is performed in reverse order Make sure that the magnet on the piston in the lower part is coupled with the magnet on the spindle in the upper part After the assembly gt Carry out a gravimetric test of the systematic and random error see Technical data on p 50 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly For the replacement of an ejector sleeve the sizes 5 mL and 10 mL are delivered with an ejector sleeve and ejector carrier The lower part has to be removed for the installation of a new ejector carrier Eppendorf Xplorer amp Operating manual 6 Maintenance 6 4 3 Multi channel Loosen and open the 10 pL 100 uL and 300 pL multi channel lower part SE 4 and slide the lever on the lower part to the left or right Pull the lower part down to cancel the magnetic coupling of the lower part to the upper part This separates the lower part from the upper part so that it can be removed Put down the lower part with the lever facing downwards slide the two latches right and left at the side down Use a coin to do this The lower part is still lying on the table wi
43. e highest precision and accuracy we recommend that you follow the following hints 5 6 1 Preparation Reagent reservoir Tip Tub Always place the liquid in a suitable tube For multi channel pipettes we recommend the reagent reservoir Tip Tub as a liquid reservoir The liquid and the pipette should have approximately the same temperature gt Use epT I P S LoRetention for solutions containing surfactants to minimize residual moisture in the tip after dispensing Use ep Dualfilter T I P S to prevent contamination by aerosols in the tip Use the pipette tips only once Check whether the set aspiration and dispensing speeds are suitable for the liquid The blow out blow is carried out at approximately the same speed 23 0 40 Operation 24 Eppendorf Xplorer amp Operating manual 5 Operation 5 6 2 Aspirating liquid 5 6 3 Dispensing liquid on Wet the new pipette tip by aspirating and dispensing the liquid to be pipetted one to three times Immerse the pipette tip into the liquid vertically approx 4 mm during aspiration Press the rocker up to aspirate liquid Maintain the immersion depth so that no air is aspirated accidentally After aspiration keep the pipette tips in the liquid for approx 3 seconds in the case of large volumes ddd After aspiration pull the pipette tip slowly out of the liquid Wipe the pipette tip slowly against the tube wall to ensure that no o
44. ected Then charge for an additional 3 hours Battery state a Battery empty Usage not possible 3 hours charging time dl The rechargeable battery is almost completely discharged The remaining rechargeable battery capacity is only sufficient for displaying the information to the left Dispensing is not possible The rechargeable battery must be charged for at least 15 minutes before further use A charging time of 3 hours is recommended Prior to aspiration the following appears temporarily Selected volume not possible with stored adjustment A factor which was determined during the adjustment of the Xplorer leads to a very high stroke increase ddd This high stroke increase limits the dispensing volume Confirm the error message with the OK softkey The only remedy is to change the adjustment Make sure that you are authorized to carry out the adjustment For the selected volume the following appears temporarily Volume limit RS uL dml The Volume limit option was used to limit the volume to the displayed value Make sure that your are allowed to change the Volume limit option 2 Move the selection dial to Opt 3 Select the Volume limit option Regularly check the precision and accuracy of the Xplorer pipette to avoid dispensing errors You can use the PICASO software from version 2 3 18 for determining the maximum permissible Systematic and random error
45. eed for aspiration and dispensing Eppendorf Xplorer amp Operating manual 5 Operation 5 5 Using pipette tips d The liquid to be dosed is aspirated into pipette tips It is recommended to use epT I P S The epT I P S pipette tips are available in different purities as tips with and without a filter and as special tips When using liquids whose surface tension is lower than that of water e g due to the presence of a wetting agent in the liquid the use of the special tips epT I P S LoRetention is recommended The pipette is only functional if a pipette tip has been attached You can either attach the pipette tip by hand or directly insert the end of the pipette into a tip held in the tip storage box If attaching a pipette tip by hand it must be handled in such a way to avoid contamination and heating of the pipette tip The color of the rocker of the Xplorer pipette matches the color of the epT I P S tray Fitthe suitable pipette tip s on the tip cone applying light pressure The pipette tip is securely fitted to the tip cone when it responds with spring loaded action exceptions no spring loading action with 5 mL and 10 mL single channel pipettes and with 1 200 uL multi channel pipettes You can deactivate the spring loading action of the tip cone in the case of single channel pipettes see p 38 5 6 Tips for correct pipetting To achieve th
46. endorf North America Inc Phone 1 516 334 7500 Fax 1 516 334 7506 E mail info eppendorf com Internet www eppendorfna com Internet www eppendorf com worldwide d Operating Manual Evaluate your operating manual www eppendorf com manualfeedback eppendorf In touch with life Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 538 01 0 Fax 49 40 538 01 556 E Mail eppendorf eppendorf com Eppendorf North America Inc 102 Motor Parkway Suite 410 Hauppauge NY 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E Mail info eppendorf com Application Support Europe International Tel 49 1803 666 789 E Mail support eppendorf com North America Tel 1 800 645 3050 menu option 2 E Mail techserv eppendorf com Asia Pacific Tel 60 3 8023 6869 E Mail support asiapacificOeppendort com
47. enu and change the values of parameters The color of the rocker is different for the various volumes and matches the color of the trays for the suitable epT I P S pipette tips 7 Ejector The ejector moves the ejector sleeve and ejects the pipette tip After a filled pipette tip has been ejected the piston returns to the basic position again after a slight delay This option can be switched off see p 30 8 Serial number The serial number is located at the lower end of the upper part 9 Ejector sleeve The ejector sleeve is marked with the nominal volume of the pipette 10 Spring loaded tip cone The spring loading action optimizes the force required for attaching and ejecting tips The spring loading action is available for all Xplorer pipettes up to 1 000 uL 11 Pipette tip The Xplorer pipettes must only be used with the suitable pipette tip fitted It is recommended to use epT I P S Eppendorf Xplorer amp Operating manual 2 Product description 2 1 2 Multi channel eppendorf For a description of the upper part please refer to the total illustration of the single channel pipette see p 8 1 Lever The lever is used to release the multi channel lower part 2 Multi channel lower part The multi channel lower part is freely rotatable It is not detached when rotated The outer channels are marked with the numbers 1 and 8 or
48. er amp Operating manual 1 User instructions Rechargeable lithium polymer battery Remaining stroke Reverse stroke Vapor pressure Viscosity Wall dispensing R This rechargeable battery is a special version of the rechargeable lithium ion battery Like rechargeable lithium ion batteries rechargeable lithium polymer batteries have a very high charging capacity and a long service life The special encapsulation of the rechargeable lithium polymer battery makes it lighter than rechargeable lithium ion batteries After completion of the dispensing steps there is still a small amount of liquid in the pipette tip as a safety reserve This is referred to as the remaining stroke The liquid of the remaining stroke can be discarded or reused by pressing the rocker in the desired direction After aspiration the piston is moved into a defined position The reverse stroke is not a dispensing step V This term refers to the pressure exerted by the vapor of a material solid or liquid in an enclosed container The vapor is in equilibrium with the solid or liquid phase of the same substance The vapor pressure increases when the temperature increases Each pure liquid has a vapor pressure of 1013 hPa mbar at boiling point Volume errors caused by high vapor pressure can be reduced by prewetting the tip Viscosity describes the viscidity of liquids and suspensions Dynamic or absolute viscosity is now usually given in Pa sor mPa
49. erate the rocker up and down several times There should not be any unusual running noises Keep the ejector pressed The ejector rod protrudes from the upper part Fit the ejector sleeve onto the ejector rod It is fitted correctly if it engages slightly Carry out a gravimetric test of the systematic and random error see Technical data on p 50 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 39 Eppendorf Xplorer amp Operating manual 6 Maintenance 6 4 2 5 mL and 10 ml single channel Maintenance 40 The piston must be in its basic position before separating the lovver and upper part Press the rocker down to move the piston to its basic position 1 unscrew the ejector sleeve 2 keep the ejector pressed and turn the lower part to the left or right by approximately 30 Pull the lower part down to cancel the magnetic coupling of the lower part The lower part is separated from the upper part 3 open the lower part fit the pipette key included in the delivery package onto the lower part Hold the cylinder and unscrew it from the lower part The cylinder with the tip cone is separated from the upper part of the lower part The upper part of the lower part and the piston cannot be disassembled any further See the next figure The lower part consists of th
50. ette You must carry out a gravimetric test to check the validity of the factor e 2 point adjustment After you enter the density two different volumes and the corresponding weighing result the Xplorer pipette will determine a correction factor The factor is only correct for the selected volume range and the selected work technique However the factor is used for the entire volume range of the Xplorer pipette i e also below and above the two measuring points You must carry out a gravimetric test to check the validity of the factor S point adjustment After you enter the density three different volumes and the corresponding weighing results the Xplorer pipette determines two correction factors The factors are correct from measuring point to measuring point in the selected volume sections and for the selected work technique The respective factor is however also used below and above the first or third measuring point You must carry out a gravimetric test to check the validity of the factors Eppendorf Xplorer amp Operating manual 5 Operation d Each Xplorer pipette whose factory setting has been changed by selection or by performing the above mentioned adjustments must be gravimetrically tested by you This is the only way of ensuring that the selected adjustment meets the requirements regarding errors 5 12 8 Language Mark a language in the selection list with the rocker The selected language is only activated
51. f further action QU ome rb com c Aspirate the same liquid from the same reservoir tube gt Press the rocker up again to aspirate the same liquid The residual liquid in the tip is used for further processing Dispense a different liquid 1 Press the rocker down to discard the residual liquid in the tip 2 Eject the pipette tip Use a new pipette tip for the next dispensing operation Eppendorf Xplorer amp Operating manual 5 Operation 5 10 Pipetting and mixing Aspiration Dispensing Release the rocker Blovv out Automatic mixing The P M mode is used to aspirate a liquid and to dispense it again Afterwards an adjustable number of mixing cycles is automatically triggered The mixing cycles can be continued as often as required 1 Prepare the Xplorer pipette see Preparing the Xplorer on p 20 2 Press the rocker up to aspirate liquid Stopping dispensing Press the rocker down to stop aspiration Press the rocker up to stop dispensing or the current mixing cycles 3 Press the rocker down to dispense the liquid and to start the selected mixing cycles The mixing cycles shown in the main field of the display under Cycles are reduced with each cycle by 1 If required you can keep the rocker pressed to perform an unlimited number of mixing cycles When you release the rocker the ongoing mixing cycle is ended after the piston h
52. g burning melting short circuiting of the Xplorer pipette or similar damage Only use the supplied power supply for charging the device You can recognize the correct power supply unit by the Eppendorf logo and the device name on the power supply NOTICE Loss of full rechargeable battery charging capacity if charged incorrectly The supplied rechargeable battery is not charged completely If the rechargeable battery is not charged completely before it is used for the first time its charging capacity will remain permanently greatly reduced The rechargeable battery will only achieve its full capacity after a few discharge charge cycles Charge the rechargeable battery for at least three hours before it is used for the first time gt Do not charge the rechargeable battery in a hot location 260 C Only charge the rechargeable battery using the supplied power supply To identify the power supply unit each unit is provided with a label with the names Multipette stream Xstream Repeater stream Xstream and Xplorer The power supply unit is designed for the range from 100 240 V eppendorf 4986 603 005 Multipette stream Xstream Repeater stream Xstream Xplorer Recharging the rechargeable battery 1 Insert the assembled power supply unit order number 4986 603 005 into a power outlet 2 In the Xplorer pipette check whether a rechargeable battery is inserted and connected 3 Insert the charging plu
53. g of the power supply unit into the connector socket of the Xplorer pipette The display briefly shows the connection with the power supply unit The charging process is indicated in the display by a flashing rechargeable battery symbol If the rechargeable battery is almost completely discharged the display will only switch on after a slight delay 4 Charge the rechargeable battery for at least three hours The rechargeable battery will only achieve its full capacity after a few discharge charge cycles 17 uorjejjejsuj Installation Eppendorf Xplorer amp Operating manual 4 Installation f CD For storing and charging the single channel and multi channel Xplorer pipettes you can alternatively use a series 4880 charger stand or a charging carousel see ordering information on the Xplorer CD Charging stand 4880 Charging carousel 4880 Eppendorf Xplorer amp Operating manual 5 Operation 5 1 Principle of operation Each Xplorer pipette has 5 different operation modes and a higher level mode for configuring the Xplorer pipette The adjustable parameters and the operational procedures differ in the operating modes During operation the piston in the pipette is moved according to how the rocker is actuated When you press the rocker up the piston in the pipette moves up Liquid can be aspirated into the attached pipette tip When you press the rocker down the piston in
54. ice from missing pipette tips Only use the Xplorer pipette with fitted pipette tips Carry over contamination and incorrect dispensing results due to the incorrect use of pipette tips The pipette tips are for single use only Prolonged use can have a negative impact on dispensing tasks Use the pipette tips only once gt Do not autoclave the epT I P S Dualfilter NOTICE Incorrect dispensing volume with special liquids and from temperature differences Solutions which differ greatly from water in terms of their physical data or temperature differences between the pipette pipette tip and liquid can result in incorrect dispensing volumes Avoid temperature differences between pipette pipette tip and liquid Make sure that the temperature is constant between 20 and 25 C and at 0 5 C Check the dispensing volume and make sure that you can affirm all the questions listed in the general information Damage to device from penetration of liquids Do not deposit the Xplorer pipette with a filled pipette tip gt Do not allow any liquids to penetrate the inside of the housing NOTICE Ifliquid has penetrated the inside of the housing have the upper part of the Xplorer pipette repaired only by service partners of Eppendorf AG Contact your responsible sales office before returning a product 3 3 Information on product liability In the following cases the protection provided by the device
55. ip cone of the channels is acentric to the cylinder The channels on the opposite side are therefore positioned mirror inverted Ensure that the channels are inserted so that they are aligned in the center again during subsequent assembly Completely press down the guiding rail for the piston rods Press the piston rod lightly out of the guiding rail and remove the channels 43 Maintenance 44 Eppendorf Xplorer amp Operating manual 6 Maintenance 6 Before installation slide the piston into the cylinder Install the channels in reverse order in accordance with the previously mentioned instructions After installing the channels carefully push the guiding rail with the piston rods upwards to ensure that the magnetic coupling of the lower and upper part can be reestablished safely 7 Insert the reassembled lower part into the upper part The lower part automatically snaps into the upper part on insertion Make sure that the magnet on the piston actuator in the lower part is coupled with the magnet on the spindle in the upper part 8 Carry out a gravimetric test of the systematic and random error see p 50 This test ensures that no parts were confused during assembly and that the pipette has been assembled correctly 6 5 Maintenance We recommend that you have all maintenance and servicing carried out by Eppendorf AG or Eppendorf service partners No warranty is
56. ked stops on the housing case and the red marked teeth on the inner part are adjacent If the stops and teeth are on top of each other after assembly the housing case has been attached incorrectly Eppendorf Xplorer amp Operating manual 6 Maintenance Removing and installing 1 200 uL channels 1 Housing case 2 Piston actuator 3 Inner part 4 Piston rod in the guiding rail of the piston 1 actuator 5 Channel consisting of a piston a cylinder and a tip cone 6 Fixing strip 7 Base plate 8 Unlocking tool for 1 200 uL lower part 2 3 4 A LJ 5 E SS 6 1 8 7 L 8 Only disassemble the channels when the upper part is detached from the lower part 1 Press the unlocking tool as far as it will go into the base plate The base plate can now be detached by pulling gently at the side at the side with the unlocking tool inserted This loosens the base plate on the other side Remove the base plate Take the inner part out of the housing case Loosen the fixing strip from the inner part To do this push the fixing strip away from the inner part in the area near to the three catches of the fixing strip on the inner part Have a close look at the channel arrangement in the inner part Four channels are accessible from each side The t
57. led water check the following s there any liquid dripping from the tip s the pipette tip fitted leak proof Adequate leak tightness is ensured when no drop is formed at the pipette tip after aspiration of the nominal volume with distilled water and a waiting time of approx 15 s Hold the pipette vertically making sure not to touch the pipette tip Prewet the pipette tip several times s the tip cone undamaged Are the piston and the cylinder leak proof Does the temperature of the pipetted liquid correspond to the temperature of the device the ambient air temperature s the weighing location free from drafts 45 uo uorjeuuojur General information on adjustment 46 Eppendorf Xplorer amp Operating manual 7 General information on adjustment Does the work method and pipetting speed permit complete aspiration and dispensing of the liquid Has the correct numerical value for Density liquids at weighing temperature been used for the calculation of the actual volume s the set volume correct For very small volumes 10 uL is the fine balance sufficiently sensitive balance resolution 0 001 mg Were original epT I P S pipette tips used for testing Refer to the chapter Technical data in the operating manual for information on which pipette tip is to be used as the test tip for checking the technical
58. lti channel lower pari 43 Reverse pipettilg ikk kk kaka KAKA 28 S Select Auto dispensing uui kk kek kek 25 DISPENSING 455a 26 operating mode ii 1 1 1 20 Set 1 33 ed ndie n z akama 20 Speed table kek keke 53 Single channel seen 53 Spring Mechanism sese 38 Standard pipetting kk eee 27 Status me 12 SterilizatiOH EE 36 SIOFAGO EE 49 E en E EE 19 1 333 0 19 Using pipette tips auana aaa annnaa nane ee vene ee vere ee ver re ven n ee eee rete 23 VV Waranty AES 10 55 xapul Index Eppendorf Xplorer Operating manual 56 QY H V C ce C v e C e C Ce BC Kou ka kak e KK E C ce qe C E EEE n iu C n e C ve Ce XWA C C ce K t cec KS E C ce C EG te CE ce qe C ce E ce C 66 Kon ot e ce C ce SE e C TR EC formi qe C ce C e C Ce YY e C Con mit qe C ce K e C ces ERR T x formi tserkl eS ce C ce c ce c YES E ity l ru CE ce E e C e C LEN e C 6 RE an hnete Produ De ng ze C ze C SEA ce ce C reiner non und No clara a e C ce C c c c SS Ach produ nen und omen Bel ene t 0n c ce cf ce c ce AS id regius Sieg m i S gru E ce ce y e C ce oF faa
59. nge the dispensing volumes and will affect the accuracy of the Xplorer pipette Carry out a gravimetric test of the adjustment d Each Xplorer pipette whose adjustment has been changed must also be marked with a clearly visible label indicating the type of change made This way you can ensure that other users will be informed of the changes to the adjustment in addition to the information provided in the header of the display You can overwrite a selected adjustment by selecting another adjustment The symbols which then apply to the new adjustment are displayed in the header of the display f CD Instructions on performing the adjustment can be found in the document Adjustment on the Xplorer CD You can adapt the adjustment of the Xplorer pipette to meet your requirements If you have changed the factory setting the header of the display will show the wrench symbol indicating the change made by the user to the adjustment followed by at least one other symbol Before changing an adjustment make sure to read the chapters Display see p 11 and General information about adjustment see p 45 The following adjustments can be selected Factory settings Resets the adjustment to the factory setting Liquid type Ethanol 7596 The factory setting is changed by a factor in order to allow 75 ethanol to be dispensed with higher accuracy The internally used factor takes into account the density and the foll
60. or pressure etc If the density of an aqueous solution changes by approximately 10 for example because of the salt concentration the volume will change by approximately 0 2 This statement does not apply if other relevant properties of the liquid also change If the pipette is used at extremely high altitudes it must be calibrated to the ambient air pressure At 1 000 m above sea level the volume error of a 100 uL pipette is about 0 396 When using tips which are very different to standard tips with regard to their geometry changing the adjustment can improve the dispensing accuracy systematic error You can reset a changed adjustment by means of a few simple steps Changes to the adjustment will not affect the dispensing precision random error Precision random error can be improved by replacing worn parts Precision random error is also strongly influenced by handling Before making changes to the adjustment you must check the existing adjustment in the factory setting with distilled water The actual volume can be checked by weighing Mean value of the weighings Actual volume Density liquids at weighing temperature The density of distilled water is approx 0 9982 mgjul at 20 C and 0 9965 mg uL at 27 C If the set volume corresponds to the actual volume no correction is necessary If there is a difference between the actual volume and the set volume of distil
61. owing work technique in the Pip mode 1 Wet the tip with liquid three times at room temperature Hold the Xplorer pipette in an upright position Avoid outer wetting during dispensing 2 Use speed level 5 standard setting for aspiration and dispensing 3 Dispense liquid against the tube wall of the destination tube 4 Trigger a blow out blow approx 2 seconds after dispensing Then eject the tip Liquid type Glycerol 5096 The factory setting is changed by a factor in order to allow 5096 glycerol to be dispensed with higher accuracy The internally used factor takes into account the density of the 5096 glycerol and the same work technique as described previously for Ethanol 7596 in the Pip mode epTIPS long In addition to the standard tips a second longer tip is available for most Xplorer pipettes When epTIPS long is selected the tip geometry of this longer tip is considered in the internal volume calculation This reduces the systematic error of dispensing when using these tips 33 0 40 Operation 34 Eppendorf Xplorer amp Operating manual 5 Operation Xplorer color code The adjustment for epTIPS long applies to Nominal volume Xplorer rocker color code Model Length Volume range epT I P S ept I P S epT I P S 10 uL medium gray light gray 20 uL long 46 mm 0 5 10 uL 100 uL yellow orange 300 ul 55 mm 5 100 uL 300 UL orange not available 15 300 uL 1 000 uL
62. performed Optional setting in the Pip mode An explanation of all dispensing parameters can be found in the chapter Setting parameters see p 20 Eppendorf Xplorer amp Operating manual 2 Product description 2 6 Materials A Aggressive substances can damage the device dispensing unit and accessories AN Check for material compatibility before using organic solvents and aggressive chemicals d b CAUTION Follow the cleaning instructions see Cleaning on p 36 The user accessible components of the Xplorer pipette are made of the following materials Component Material External surfaces of the upper part Highly purified polypropylene PP Polycarbonate PC PC coated PC tinted Gold plating Exterior and interior of lower parts Highly purified polypropylene PP Polyvinylidene fluoride PVDF Polyetherimide PEI Polyphenylene sulfide PPS Polyetheretherketone PEEK Polytetrafluorethylene PTFE Ethylene propylene diene M class rubber EPDM Silicone Steel stainless steel and spring steel Pipette tip Material epT I P S Polypropylene PP epDualfilter T I P S filter Polyethylene PE 13 uonduosap jpanpold Safety Eppendorf Xplorer amp Operating manual 8 1 Intended use The Xplorer pipette is a lab device intended for dispensing liquids in the volume range from 0 5 uL to 10 mL in combination
63. plug in power plug adapters Input voltage 100 V to 240 V 10 50 60 Hz Output voltage 5V 1A Xplorer Operating temperature 5 to 40 C Air humidity during operation 10 to 95 rel humidity Storage temperature 5 to 45 C Air humidity during storage 10 to 95 rel humidity Weight without rechargeable battery without pipette tip Single channel 100 uL approx 135 g Eight channel 100 uL approx 213 g Eppendorf Xplorer amp Operating manual 10 3 Speed table for Xplorer single channel 10 Technical data The following speed table is valid for Xplorer pipettes with the software version 01 05 00 Speed 10 ul 100 ul 300 ul 1 000 pl 5mL 10 mL level Speed Time sec Time sec Time sec Time sec Time sec Time sec 1 12 01 12 01 12 01 12 01 12 01 12 01 2 8 0 8 0 8 0 8 0 8 0 8 0 3 4 0 4 0 4 0 4 0 6 01 6 01 4 2 8 2 8 2 8 2 8 4 8 4 8 5 2 2 2 2 2 2 2 2 3 6 3 6 6 1 6 1 6 1 6 1 6 3 2 3 2 7 1 2 1 2 1 2 1 2 2 8 2 8 8 0 86 0 87 0 87 0 85 2 6 2 6 10 4 Speed table for Xplorer multi channel The following speed table is valid for Xplorer pipettes with the software version 01 05 00 Speed level 10 pl 100 ul 300 ul 1200 uL Speed Time sec Time sec Time sec Time sec 1 12 01 12 01 12 01 12 01 2 8 0 8 0 8 0 8 0 3 4 0 4 0 4 0 4 0 4 2 8 2 8 2 8 2 8 5
64. provided for any damage due to misuse or if the device has been opened by unauthorized persons Eppendorf Xplorer amp Operating manual 7 General information on adjustment NOTICE Incorrect dispensing volume with special liquids and from temperature differences Solutions which differ greatly from water in terms of their physical data or temperature differences between the pipette pipette tip and liquid can result in incorrect dispensing volumes Avoid temperature differences between pipette pipette tip and liquid Make sure that the temperature is constant between 20 and 25 C and at 0 5 C gt Check the dispensing volume and make sure that you can affirm all the questions listed in the general information Information on the random and systematic errors determined before delivery can be found in the enclosed Eppendorf Certificate If the factory setting has been replaced by a different adjustment this renders the validity of the Eppendorf Certificate invalid If you reset the adjustment to the factory setting the random and systematic errors determined before delivery and the enclosed Eppendorf Certificate will apply again The Xplorer pipette was adjusted before delivery and tested with aqua bidest according to ISO 8655 Changing the adjustment of the Xplorer pipette is sometimes recommended for solutions which are very different from water with regard to their density viscosity surface tension and or vap
65. rdering information see the Xplorer CD Procedure Follow the operating manual of the autoclave manufacturer 1 Autoclave at 121 C 20 minutes 1 bar pressure 2 Putthe lower part into the autoclave in such a way to ensure that the temperature at the lower part does not exceed 121 C 3 You can put the lower part into the autoclave as a whole unit or as individual parts Make sure that no parts of disassembled lower parts are confused during subsequent assembly recommendation use a plastic beaker for each lower part Eppendorf Xplorer amp Operating manual 6 Maintenance After autoclaving 6 2 2 1 Disinfection Cool the lovver part down to room temperature and leave to dry The piston does not need to be greased follovving autoclaving Carry out a gravimetric test to check that the Xplorer pipette is functioning Carefully wipe off the outer surfaces with disinfectant DNA RNA decontamination agents or 70 isopropanol You can wipe the outside of the tip cone and the ejector sleeve with a sodium hypochlorite solution of 4 After the exposure time of the sodium hypochlorite solution has elapsed thoroughly remove it using demineralized water 6 3 Replacing O rings 6 3 1 6 3 2 The 100 uL 300 uL and 1 200 pL multi channel lower parts are equipped with O rings These are wear parts Replace old worn or damaged O rings Defective O rings result in the incorrect positioning of the tips and in di
66. s provided for any damage due to misuse or if the upper part has been opened by unauthorized persons The Rechargeable lithium polymer battery and all other wear parts are excluded from the warranty Eppendorf Xplorer amp Operating manual 2 Product description 2 5 Display d You can change the brightness of the display in the Options The display dims during usage intervals and switches off after a prolonged usage interval Touch any key to return the dimmed or switched off display back to the defined brightness If the selection dial is set to Off you first have to set the selection dial to an operating mode 1 Pipetting A 5004 Speed Counter _ 3 0x Edit ep Help 4 1 Header 2 Status line 3 Main field 4 Footer Header Adjustment If you changed the factory setting a wrench symbol will appear in the left part of the header If the factory setting is active no symbol will appear in the header Additional symbols on the right indicate which adjustment has been set in the Options 1 point adjustment Single point adjustment by the user 2 point adjustment Two point adjustment by the user 3 point adjustment Three point adjustment by the user Glycerol 5096 Adjustment to liquid type 50 glycerol Ethanol 7596 Adjustment to liquid type 75 ethanol Altitude Adjustment to an altitude other than 0 m above sea level epTIPS long Adju
67. spensing errors Removing the O ring 1 Push the opening of the O ring tool included in the delivery package against the tip cone from the side so that the sharp edge in the opening of the O ring tool is positioned on top of the o ring Do not slide the O ring tool onto the cone The figure shows the O ring tool for 100 uL and 300 uL A separate O ring tool is available for 1 200 uL Supporting the O ring tool with your thumb push it firmly against the tip cone This O ring is cut in one place 3 Remove the O ring tool and the O ring from the tip cone Mounting a new O ring ila Fit the mounting aid shortened pipette tip included in the delivery package of the O rings on the tip cone Push the new O ring over the tip onto the tip cone Check that the pipette tips are correctly positioned Ensure that the tips are tightly sealed and properly aligned 37 Maintenance 38 Eppendorf Xplorer amp Operating manual 6 Maintenance 6 4 Xplorer pipette disassembly and assembly 6 4 1 Single channel up to 1 000 pL Removing the lower part 1 Keep the ejector pressed and remove the ejector sleeve 2 and G on the lower part slide the ring marked PUSH UP TO RELEASE up by about 5 mm until the lower part comes off 3 take the lower part out of the upper part Deactivating the spring loading action inst
68. stment to an epT I P S long Sound volume You can change the sound volume setting for the acoustic signals ER in the Options see p 30 If the volume has been set to D no loudspeaker symbol is displayed Rechargeable battery Information on the charging capacity of the rechargeable lithium polymer battery El Rechargeable battery fully charged E Rechargeable battery partially discharged mJ Rechargeable battery almost discharged Rechargeable battery must be charged see p 17 11 uonduosap jpanpold Product description 12 Eppendorf Xplorer amp Operating manual 2 Product description Status line Selected operating mode The selected operating mode is displayed here during operation In edit mode the name of the parameter to be edited is displayed In the Edit mode the status line shows the name of the parameter to be edited The parameter to be edited is also displayed outlined and marked in red in the main field Footer Assignment of the softkeys with variable functions Edit Open the edit mode Next Select next parameter Help Show help End Exit the edit mode or help Main field All the dispensing parameters of an operating mode are displayed at the same time In edit mode the parameter to be edited is marked in red Some of the dispensing parameters in the main field are The dispensing volume Speed 8 speed levels for aspiration and dispensing Counter Number of dispensing operations
69. th the lever facing downwards Remove the cover panel facing upwards with the integrated ejector rail Do not remove the ejector rail from the cover plate The spring for the ejector rail could accidentally come off and get lost in this step Removing and installing channels Channels must only be removed and installed if the multi channel lower part is separated from the upper part The channels in the lower parts consist of a piston a cylinder and a spring The channels for 100 uL and 300 pL are equipped with an O ring at the tip cone see Replacing O rings on p 37 Gja Ss d U i ES Te i Te B z to o B o and position a pipette tip under the piston and carefully take the piston off the upper rail Carefully pull the piston out in an upward direction Do not bend the piston Hold the tip cone at the lower end and push it slightly upwards This compresses the spring lift the tip cone slightly and release it from the lower rail G relax the spring by letting the tip cone slide back again above the lower rail G take the tip cone with
70. the Ads mode an ongoing dispensing step is completed after the rocker is released The next dispensing step is only performed when the rocker is pressed accordingly Eppendorf Xplorer amp Operating manual 5 Operation 5 7 Automatic dispensing Aspiration The Ads mode is used to aspirate a liquid and dispense it in partial volumes of equal size in a selected interval The reverse stroke and remaining stroke are triggered before or after the dispensing steps These additional volumes may result in volume limits when using filter tips see p 31 P Sa E gt ce Reverse stroke Automatic dispensing Aspirate or empty 1 Prepare the Xplorer pipette see Preparing the Xplorer on p 20 2 Press the rocker up to aspirate liquid Then follow the instructions in the display To abort dispensing you can press the Empty softkey The tip is completely emptied 3 Press the rocker down to trigger the reverse stroke 4 Keep the rocker pressed for the duration of dispensing The following dispensing steps will be triggered after the first dispensing step with the time interval that has been specified in the Time parameter When you release the rocker an ongoing dispensing operation is still ended The display shows the remaining dispensing operations under Steps Press the rocker again to continue dispensing After dispensing deci
71. tion settings and the software version during a hardware reset If the piston is not in its basic position during the hardware reset you will be prompted in the 0 display to press the rocker down Make sure that any liquid in the pipette tip is dispensed correctly 8 2 Error search Jj Symptom Possible cause Solution The display is dark The pipette is in sleep mode Connect the rechargeable battery The rechargeable battery is not Press a softkey or the rocker connected gt Charge the Xplorer pipette The rechargeable battery is Note If the rechargeable battery is almost discharged completely discharged the display will only switch on after a short charging time Liquid is dripping from the tip The pipette tip is loose or does not fit gt Attach the tip firmly and or the dispensed volume correctly gt Use epT I P S is incorrect Liquid with high vapor pressure and or Wet the tip several times and adjust the different density pipette for the liquid used Pipetted too quickly gt Set a lower speed Tip withdrawn from the liquid too early or Withdraw the tip from the liquid slowly too quickly and with a time delay of about 3 seconds for large volumes after aspiration Liquid dispensed without blow out in Pip Trigger blow out after dispensing has mode been completed during standard pipetting applications The piston is soiled or damaged gt Clean the piston relubricate slightly and or replace The tip cone is
72. um gray 1uL t2 5 0 025 1 8 0 018 E Increment 0 1 20 uL 5 uL 1 5 0 075 0 8 0 04 GO KE 10 uL 1 0 0 1 0 4 0 04 5 100 uL yellow 10 uL 2 0 0 2 1 0 0 1 Increment 2 200 uL 50 uL 1 0 0 5 0 3 0 15 Bat ue PUR 100uL 140 8 0 8 0 2 0 2 15 300 uL orange 30 uL 2 5 0 75 0 7 0 21 Increment 15 300 uL 150 uL 1 0 1 5 0 3 0 45 la sp mmi 300uL 0 6 1 8 0 2 0 6 50 1 000 pL blue 100 uL 3 0 3 0 0 6 0 6 Increment 50 1 000 HL 500 uL 1 0 5 0 0 2 1 Li a CR 1000uL np 6 0 0 2 2 0 25 5 mL purple 0 5 mL 3 0 15 0 0 6 3 Increment 0 1 5 mL 2 5 mL 1 2 30 0 0 25 6 25 SEH TERRE 5mL 0 6 30 0 0 15 7 5 0 5 10 mL turquoise 1mL 3 0 30 0 t 0 60 6 0 Increment 1 10 ml 5mL 0 8 40 0 0 20 10 0 SE Homa 10 mL 0 6 60 0 0 15 15 0 50 10 Technical data Eppendorf Xplorer amp Operating manual 10 2 Eppendorf Xplorer multi channel d The technical data applies to both the 8 channel and the 12 channel pipette Model Test tip Testing Eppendorf AG error limits Increment epT P S colorcode Volume Error volume range Systematic Random Length 96 pL pL 0 5 10 uL medium gray 1 uL 5 0 0 05 3 0 0 03 Increment 0 1 20 uL 5 uL 3 0 0 15 1 5 0 075 OJ SM 10 pL 2 0 0 2 0 8 0 08 5 100 uL yellow 10 uL 2 0 0 2 2
73. uter wetting remains on the pipette tip When dispensing liquid place the pipette tip at a slight angle against the wall of the tube Press the rocker down to dispense the liquid After dispensing wait until no more liquid is dispensed Press the rocker down again to trigger the blow out blow The following information applies especially to high viscous solutions gt During the blow out blow keep the rocker pressed down if the liquid is flowing out at a slow rate so that the liquid can flow out safely If you keep the rocker pressed during the blow out blow the piston in the pipette will remain at its lowest position gt If present wipe any outer wetting against the wall of the tube while holding the rocker pressed down Leave the dispensing vessel with the rocker pressed down Only release the rocker outside of the dispensing vessel The piston only moves to its basic position when you release the rocker Torepeat the blow out blow if necessary press the rocker down again Stopping the piston movement Pressing the rocker in the other direction when the piston is still moving will stop the piston movement You can then move the piston either up or down by pressing the rocker in the corresponding direction A mixing process is aborted by pressing the rocker up Operating modes with the rocker pressed down In the Man mode the ongoing piston movement is immediately stopped by releasing the rocker In
74. x 1 000 pL single channel Eppendorf Xplorer pipettes 1 For multi channel pipettes from 100 UL Multi channel tool for cutting the O rings on the tip cones 1 Unlocking tool for opening the multi channel lower part of 1 200 uL Eppendorf Xplorer pipettes 1 Pipette wrench for opening the lower part of 5 mL and 10 mL Eppendorf Xplorer pipettes 2 3 Features The Xplorer pipettes are motorized and electronically controlled piston stroke pipettes designed to aspirate and dispense liquid volumes according to the air displacement principle The Xplorer pipette family includes single channel and multi channel pipettes 8 or 12 channels Depending on the Xplorer pipette you can dispense volumes ranging from 0 5 uL to 10 mL The liquid is aspirated into a pipette tip which is attached to the Xplorer pipette The main control element of the Xplorer pipette is a rocker The color of the rocker differs depending on the volume range of the Xplorer pipette It has different functions during dispensing operations and in the dispensing parameter editing mode The rocker can either be pressed up or down The lower parts of the Xplorer pipettes are autoclavable For all Xplorer pipettes from 10 uL to 1 000 uL nominal volumes the tip cone responds with spring loading action when a pipette tip is attached 24 Warranty d In case of warranty claims contact your local Eppendorf contractual partner No warranty i

Eppendorf Xplorer® - Operating manual

Contents

Download Pdf Manuals

Related Search

Related Contents

Eppendorf Xplorer&reg; - Operating manual

Contents

Download Pdf Manuals

Related Search

Related Contents

Eppendorf Xplorer® - Operating manual