Intended Use
Contents
1. Abbreviations and Terminology Here you can find information on the abbreviations and terminology used in this manual High Pressure Gradient Operating mode of an HPLC sys tem The solvent is mixed on the high pressure side of the pump HPLC High Performance Liquid Chromatography Solvent Mobile phase eluent or carrier for liquid chromatography Remote External control with chromatography software or analog control signals P 2 1S P 4 15 User Manual V6870 Version 2 0 46 Index A Accessories 9 Additives 8 AVV marking 43 C Cap fitting 15 Care 36 Check valves Cleaning 39 Exchanging 38 Removing 39 Cleaning 36 Connectors on the Rear Side 16 Contact 36 Controlling the pump 22 D declaration of conformity 48 decontamination 11 Device safety 9 E Electrical connections 16 Eluent 8 Eluent line Connecting the pump head 15 Eluents 8 F Features 6 Flushing the pump 22 l Installation 13 Installation location see location 13 IQ 32 L LAN port 21 problems 33 router 20 settings 18 setup 19 leak 10 Line voltage 14 Location 13 M Maintenance 36 maintenance contract 37 Modifiers 8 o Operation Arrow keys 24 OQ 32 P port LAN 21 power strip 11 supply 11 Power supply 14 power supply cable 10 professional group 9 Pump control 22 Pump head exchanging 37 Installing 38 removing 37 Pumpensteuerung 23 R Remote terminal strip 16 router LAN 20 S Safety 9 safety equip2. 2 Use the power supply to connect the router to the mains power system Configuring the Router The router is preset at the factory You will find a label at the bottom side of the router on which IP address user name and password are printed These infor mation help to open the router configuration Procedure 1 To open the router configuration start your Internet browser and enter the IP address not for all routers 2 Enter user name and password Configure the router as DHCP server In the router configuration check the IP address range and make changes if necessary Result Once the router has assigned IP addresses to all devices the chromatography software can be used to remotely control the system hw Integrating the LAN into a Company Network A network administrator can integrate the LAN into your company network In this case you use the WAN port of the router P 2 1S P 4 1S User Manual V6870 Version 2 0 Unpacking and Setup 21 Prerequisite There is a patch cable for the connection Procedure 1 Check that the IP address range of the router and of the company network do not overlap 2 In case of an overlap change the IP address range of the router 3 Use the patch cable to connect the router WAN port to the company net work 4 Restart all devices including the computer Controlling Several Systems Separately in a LAN Devices connected to a LAN communicate through ports which are p
3. CAUTION Damage to the pump head caused by overtightened cap illary fittings Note the torque of the fittings 5 Nm for stainless steel fittings 0 5 Nm for PEEK fittings Insert the fastening screws and tighten alternately Tighten the outlet fitting to the pressure sensor Tigthen the inlet fitting to the eluent Tighten the inlet fitting and the outlet fitting of the piston Check Valves Dirty check valves do not open and close properly They cause pressure fluctua tions and irregular flow Note Insert the valves in the direction of flow P 2 1S P 4 15 User Manual V6870 Version 2 0 Maintenance and Care Fig 24 Check valve Removing the Check Valves The pump head is equipped with two check valves Prerequisite The pump head has been purged The capillary and tubing have been removed The pump head has been removed Tools Open end wrench size 13 Unscrew and remove the capillary connector Loosen the outlet fitting with the open end wrench 3 Remove the first check valve Loosen the inlet fitting with the open end wrench Remove the second check valve cs Fig 25 Check valve in pump head Next steps Clean the check valves Cleaning the Check Valves 1 Fill a beaker with solvent P 2 1S P 4 15 User Manual V6870 Version 2 0 39 40 Maintenance and Care 2 Place the valve in the beaker 3 Put the beaker in an ul
4. OOOO Stop flow To control the flow rate by an external control voltage you need to select ANA LOG control in the menu J o HERNAN Fig 22 Status display Legend Pressure in 0 1 MPa Flow rate in ml min Prerequisite The pump is connected to the power supply P 2 1S P 4 15 User Manual V6870 Version 2 0 Operation 31 Procedure 1 Apply control voltage 2 Press the Start Stop button so as to start the pump The star symbol on the display of the pump indicates that the pump is working with an externally controlled flow rate 3 To stop the pump press the start stop key again Starting with a Short Circut Connection for the short circuit or TTL low for interrupting and continuing the operation of the pump The operation of the pump is dependent on the STARTLEVEL setting STARTLEVEL 1 standard setting RS 232 LAN The pump does not operate during the time of short circuit STARTLEVEL 0 standard setting Analog The pump operates during the time of short circuit Fig 23 Status display Legend Pressure in 0 1 MPa Flow rate in ml min Enter STARTLEVEL 1 in the terminal program to stop the pump during short cir cuit Prerequisite The pump is connected to the power supply Note During the interruption the horizontal arrow remains in the display because the pump is still in operation status Procedure 1 Connect the pump to a suitable terminal program 2 Conne
5. Version 2 0 26 Operation e Exceeding or undercutting the values for the maximum or minimum power consumption leads to the pump being automatically shutdown e Set the maximum power consumption for the pump without pressure sensor to limit the pump pressure e Set the minimum power consumption so as to avoid a dry run of the pump at highly reduced maximum power consumption e g if leaking The pump is preset to a standard value for the maximum power consumption KNAUER recommends that with smaller flow rates the standard value for the maximum power consumption should be insignificantly decreased Legend Maximum value Minimum value Note If the minimum is set to 0 the minimum power consumption is not monitored Press both arrow keys simultane ously until the correct display appears Let go of the arrow keys When the cursor flashes use the arrow keys again to set the value I Ol5l0 for the maximum power consump tion Hold down left arrow key Press right arrow key once Fig 16 Minimum power consumption When the cursor flashes use the arrow keys again to set the value I 000 for the minimum power consump tion Fig 17 Maximum power consumption Press start stop key once to return to the start display P 2 1S P 4 15 User Manual V6870 Version 2 0 Operation 27 Result The setting is completed If the value for maximum power consumption is exceeded the pump switches off Fl
6. pump head CORRS50 RD WR 0 300 Correction parameter for 50 ml pump head FLOW RD WR 0 50000 Writing reading the flow in ul min PRESSURE RD 0 400 Pressure readout in 0 1 MPa PMIN10 RD WR 1 400 Minimum pressure for 10 ml pump head in 0 1 MPa PMIN50 RD WR 150 Minimum pressure for 50 ml pump head in 0 1 MPa PMAX10 RD WR 0 400 Maximum pressure for 10 ml pump head in 0 1 MPa PMAX50 RD WR 0 150 Maximum pressure for 50 ml pump head in 0 1 MPa IMIN10 RD WR 0 100 Minimum motor current for 10 ml pump head IMIN50 RD WR 0 100 Minimum motor current for 50 ml pump head IMAX10 RD WR 0 100 Maximum motor current for 10 ml pump head P 2 1S P 4 1S User Manual V6870 Version 2 0 Operation 30 Control com Range and specifi Description mand cation IMAX50 RD WR 0 100 Maximum motor current for 50 ml pump head HEADTYPE RD WR 10 50 STARTLEVEL RD WR 0 1 ERRIO RD WR 0 1 STARTMODE RD WR 0 1 EXTCONTR WR 0 1 MOTOR RD0 100 oa WR REMOTE WR Controlling the Flow Rate WR WR Writing reading the pump head type Sets logical level of the START IN input to start the flow delivery Writing reading the ERROR input output OUT 0 or IN 1 0 Pump pauses after switch on 1 Pump starts with last used flow rate at switch on 0 Prevents external flow control 1 Allows flow control through ana log input 1 V 1 5 ml min
7. solvent enters the piston backflushing inform the technical support of KNAUER P 2 1S P 4 15 User Manual V6870 Version 2 0 36 Maintenance and Care Flow rate is not cor Check the following options rect Inspect the inlet and outlet screw fittings of the pump head Clean the check valves Exchange the check valves Replacing the pump head Pump without pressure sensor Pay attention to the influence of the pressure on the flow rate will not be compensated Inform the Technical Support of KNAUER Pump will not turn Power cable needs to be connected to power supply on and power adapter has to be turned on e Inspect the power cable to ensure that it is plugged into the power supply Maintenance and Care Proper maintenance of your HPLC device will ensure successful analyses and reproducible results CAUTION Intruding liquids can cause damage to the device Place solvent bottles next to the device or in a solvent tray Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol Users may perform the following maintenance tasks themselves Replacing the pump head Exchanging the check valves Contacting the Technical Support If you have any technical questions regarding the hardware or software of the manufacturer please use one of the contact options below European Technical Support
8. Hastelloy C inlays for aggressive media different pump head sizes 10 ml or 50 ml The front of the pump head is labeled with the specifications for the maximum pumping capacity 10 ml or 50 ml Pump heads with inlays carry additional material labels SST for stainless steel C for ceramics HC for Hastelloy C Labeling on the pump heads Legend flow rate performance material P 2 1S P 4 15 User Manual V6870 Version 2 0 8 Intended Use Eluents Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials Note The list of selected solvents was compiled based on research in the pertinent literature and is only a recommendation If there is any doubt contact the Technical Support of the manufac turer Not suitable eluents Halogenated hydrocarbons e g Freon Concentrated mineral and organic acids Concentrated bases Eluents containing particles Perfluorinated eluents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Less suitable eluents Dimethyl sulfoxide DMSO Slightly volatile eluents Methylene chloride Tetrahydrofuran THF Dilute phosphoric acid Suitable eluents Acetone at 4 25 C 39 2 77 0 F Acetonitrile Benzene Chloroform Ethyl acetate Ethanol Hexane heptane at 4 25 C 39 2 77 0 F Isopropanol Carbon dioxide liquid 99 999 CO2 Methan
9. Prerequisite Interface RS 232 is connected Status display is active Process Press both arrow keys simultane ously until the correct display HOL appears RS 2 32 2 Let go of the arrow keys Using the arrow keys scroll until Fig 20 Display control RS 232 is displayed Results When setting is finished the status display appears Changing to Analog Control Prerequisite Interfaces ANALOG IN and ANALOG OUT on the pin header are connected Status display is active Process Press both arrow keys simultane ously until the correct display PIC appears ANALOG Let go of the arrow keys Using the arrow keys scroll until ANALOG is displayed Fig 21 Display control P 2 1S P 4 1S User Manual V6870 Version 2 0 Operation 29 Results When setting is finished the status display appears Control commands The control commands listed below are considered for the communication with RS 232 and LAN When entering a parameter you must place a colon or space between command and parameter value e g PMIN10 100 Consider the following specifications for data transfer with RS 232 interface 9600 baud 8 bit 1 stop bit no parity check Control com Range and specifi Description mand cation ADJ10 RD WR 100 2000 Adjust parameter for 10 ml pump hea ADJ50 RD WR 100 2000 Adjust parameter for 50 ml pump hea CORR10 RD WR 0 300 Correction parameter for 10 ml
10. be established Has this been successful 8 Make sure that the IP port of the device matches the port in the chromatography software Error List and Solutions Problem Pump will not turn Power cable needs to be connected to power supply on and power adapter has to be turned on e Inspect the power cable to ensure that it is plugged into the power supply P 2 1S P 4 1S User Manual V6870 Version 2 0 Troubleshooting 35 When purging the The venting screw on the pressure sensor must be pump switches off turned up e Check if the venting screw on the pressure sensor is turned open Pump does not Check the following options transport solvent e Purge the pump head so as to remove the air bub bles Inspect the eluent inlet and filter of the HPLC column and change when blocked Replacing the pump head Clean the check valves Exchange the check valves If the pump head seals are defective solvent enters the piston backflushing inform the technical support of KNAUER Pressure or flow rate Check the following options fluctuations e Purge the pump head so as to remove the air bub bles Always tighten the inlet screw and outlet screw on the pump head with a torque wrench and 7 5 Nm Clean the check valves Exchange the check valves Pump head leaks Check the following options Inspect the inlet and outlet screw fittings of the pump head Replacing the pump head If the pump head seals are defective
11. for the flow rate 2 Check if the right value appears on the display Fig 13 Display control Result The setting is completed and the pump runs at the set flow rate P 2 1S P 4 15 User Manual V6870 Version 2 0 Operation 25 Setting the Pressure Switch Off e Set maximum pressure to avoid damaging the pump or pump head e Set minimum pressure to avoid running the pump dry Legend Maximum value Minimum value Note If the minimum is set to 0 the minimum pump pressure is not monitored Press both arrow keys simultane ously until the correct display appears Let go of the arrow keys When the cursor flashes use the arrow keys again to set the value for the makimum pressure Hold down left arrow key Press right arrow key once Fig 14 Maximum pressure When the cursor flashes use the Q arrow keys again to set the value E for the minimum pressure Hold down left arrow key Press right arrow key once to return to the start display Fig 15 Minimum pressure Result The setting is completed If the maximum pressure is exceeded the pump switches off If the minimum pressure is undercut the pump switches off after 30 s The display shows an error message in both cases Setting the Power Consumption The power consumption is dependent on the flow rate and the counter pres sure It increases with higher flow rates and stronger counter pressure P 2 1S P 4 15 User Manual V6870
12. oe Baers ag 14 UNPackING feces ols os wee Bak abah let dae todo eda ee eles 14 Connecting the Eluent Line to the Pump Head 15 Controls sioaren NG aa NG eee be RSE Se wk ee eee oa tells 16 Pin Header for Remote Control 02 0 2 e ee ee eee 16 Controlling with a Computer in a Local Area Network LAN 18 Configuring the LAN Settings 02 0 eee eee eee 18 Connecting the Cables 2 0 0 0 eee ee ee ee eee 19 Configuring the Router 0 eee eee ee eee 20 Integrating the LAN into a Company Network 20 Controlling Several Systems Separately in a LAN 21 GEO UNG sits chore ean cule Slush een SEP Een Mined ae ing PA a a eaten 21 Operation sci ses snd ba Anang a ht Sct KA A Baba as at ws Wes ata ga aay ware afiddar aan ote 22 Switching on the Pump 0 eee eee eens 22 Selecting the Pump Head 0 eee eee ee nee 22 Flushing the Pump 2 eee eee a a eee ences 22 Controle aca Maho eee a ahs Be amp eave ey eee ees 23 Keypad sasana ara NT a BN Ga EN EN Nee Swern aa ties 24 SOftWate ragnarna a wan a wae mites gala aing gala EEO na anana 27 P 2 1S P 4 1S User Manual V6870 Version 2 0 4 Table of Contents Switching Off the Pump 0 eee eee ee eee nee 32 Functionality Tests ss 0 0 ai ANAA AA ag aa Tan eee eee eee eee 32 Troubleshooting eee eee ee eee eee ee eee 33 LANG 4 oven mat Amanat ties Na
13. range from 0 001 9 999 ml min and a pressure of up to 400 bar Semi preparative pump head with a flow rate range from 0 01 50 ml min and a pressure of up to 150 bar Dual piston technology for constant flow rates Setting a limit for minimum and maximum pressure to protect the columns and to avoid a dry run of the pump only P 4 15 Emergency stop independent from control with chromatography software The pump can be controlled with the keypad in standalone mode or with the chromatography software The pump heads can be easily removed and replaced via four front accessible screws by the user Unlike the pump P 2 1S pump P 4 15S is equipped with a pressure sensor Features Liquid transport with stable flow rate and high flow accuracy Long service life P 2 1S P 4 1S User Manual V6870 Version 2 0 Intended Use 7 Pump heads completely made of stainless steel or with Hastelloy C or ceramic inlays Piston backflushing High physical and chemical stability Flexible control with LAN connection RS 232 interface and analog control signals Control with chromatography software Options A pump in combination with another pump can be used to set up a binary high pressure gradient system Pump Heads Pump heads for use in analytical applications Stainless steel with stainless steel inlays for standard applications Stainless steel with ceramics inlays for biocompatibility Stainless steel with
14. 3 Hazardous substances A device or system marked with CE fulfills the product specific requirements of European directives This is con firmed in a Declaration of Conformity Testing seals in Canada and the USA at nationally recog nized testing centers NRTL The certified device or sys tem has successfully passed the quality and security tests Unpacking and Setup Contacting the Technical Support You have various options to contact the technical support Phone 49 30 809727 111 Fax 49 30 8015010 E mail support knauer net Location Requirements Requirements The location for the device must meet the following requirements CAUTION Defect of the device due to overheating possible Set up the device in such a way that it is protected against exposure to direct sunlight Keep at least 15 cm clear at the rear and 5 10 cm at each side for air circulation Weight 1 5 kg Dimensions 105 x 100 x 185 mm Width x Height x Depth Power supply 24V DC P 2 1S P 4 1S User Manual V6870 Version 2 0 14 Unpacking and Setup Air humidity lt 90 Temperature 4 40 C 39 2 104 F Space requirements Side clearance to other devices If there is a device on one side minimum clearance of 5 cm If there are devices on both sides minimum clearance of 10 cm At least 30 cm gap to the fan on the rear of the device Power supply The device is intended for use with AC power network
15. Explanations DANGER Lethal or very serious injuries can occur WARNING Serious or moderate injuries can occur CAUTION Moderate injuries can occur Device defects can occur Decontamination Contamination of devices with toxic infectious or radioactive substances poses a hazard for all persons during operation repair sale and disposal of a device P 2 1S P 4 1S User Manual V6870 Version 2 0 12 Symbols and Signs DANGER Health danger if getting in contact with toxic infectious or radio active substances Before disposing of the device or sending it away for repair you are required to decontaminate the device adequately All contaminated devices must be properly decontaminated by a specialist com pany or the operating company before they can be recommissioned repaired sold or disposed of All materials or fluids used for decontamination must be collected separately and disposed of properly Symbols and Signs The following symbols and signs can be found on the device in the chromatog raphy software or in the user manual High voltage hazard Electric shock hazard Electrostatic discharge hazard damages to system device or components can occur General warning sign moderate injuries can occur and also damages to system device or components UV light hazard eye injuries can occur Leak hazard damages to device can occur P 2 1S P 4 15 User Manual V6870 Version 2 0 Unpacking and Setup 1
16. Hotline Languages German and English Available by telephone 8 am to 5 pm CET P 2 1S P 4 1S User Manual V6870 Version 2 0 Maintenance and Care 37 Phone 49 30 809727 111 Fax 49 30 8015010 E mail support knauer net manufacturer Proper maintenance of your HPLC device will ensure successful analyses and reproducible results Maintenance Contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract e Opening the device or removing housing parts Pump Head Removing the Pump Head Requirements The pump head has been flushed with suitable solvent WARNING A Aggressive or toxic solvent residue can irritate the skin Wear protective gloves Flush the pump head before exchanging it CAUTION Piston rods can break Before disassembling the pump head remove the two piston rods and deposit in the correct orientation When assembling the pump head the piston rods must be inserted on the same side they have been removed from P 2 1S P 4 1S User Manual V6870 Version 2 0 38 Maintenance and Care Unscrew inlet fitting and outlet fitting Unscrew the inlet fitting to the eluent Unscrew the outlet fitting to the pressure sensor Alternately unscrew the 4 fastening screws Hold the pump head and consecu tively pull out all fastening screws Installing the Pump Head
17. Ih KNAUER feura Pump P 2 1S P 4 15 User Manual V6870 Akung siaran er eS gt as pE AURE mvs nom 01 ax mum BAE KNAUER MB ae MO AO L Note The contents of this user manual apply for all BlueShadow products Please submit a request on any article numbers for BlueShadow Table of Contents 3 Table of Contents Note For your own safety read the manual and always observe the warnings and safety information on the device and in the manual INtENdER Use nice ee GAS a eae eae ws es 5 DEVICE OVERVIEW cis ih el ls a a a a ale ALAA ahaa at Bee KN GG age ay echt Ua Rta NENG 5 FOACUL ES aa a KN A KN NA NG NE ea Je se Se a ARNEE NE COE ae dines 6 PUMP HEads 5 552 024 20 etcacivscocebriuth twits eat a ah aly tls ang ana aana 7 EUERE ne see thie ae tae ed wt a ey ieee Ag a thes 8 Scope of Delivery aas a as ee RAO ee A Sie ie eee ee a 9 Safety 366 Se RGR a Kagan se gga a EN lutea ts a EA sata te ts ANG aa E val sual sues Gaga aga eg 9 Definition of Personal and Material Damage 11 DECOMPAMINATIONE saa sada gg lel ele KR REIS SUS AS AE St hd 11 Symbols and Signs 0 cece ee ee eee eee eens 12 Unpacking and Setup 0 cee cee eee eee eee eee 13 Contacting the Technical SUPPOrt 0 00 13 Location Requirements 0 ne a cee kan as eee 13 Requirements ii asa eee SNS a a eek ban doce ee ee a 13 Power SUDDIY a s saneh ani iea Woe ae Bee ee
18. LAN ENR TORR ee ee all 33 Error List and Solutions cece eee eee ee eens 34 Maintenance and Care cee cee eee eee eee eee 36 Contacting the Technical SUPPOrt 0 2 0 eee eee eee 36 Maintenance Contract a a gg ccc ee eee 37 Pump Head 0 anni tebuds i ee Ta seat tote dade dade tee es 37 Removing the Pump Head 0 2 eee eee ee ee eee 37 Installing the Pump Head 20 eee eee eee eee 38 Check ValVes sene nann janin Aan AeA SI LOE te ata E de da 38 Removing the Check Valves 0 0 e eee eee eee 39 Cleaning the Check Valves 2 2 0 0 eee eee eee eee 39 Installing the Check Valves 2 0 0 0 eee eee ee eee eee 40 Technical Datas sesser E ET Eaa a Kaga Ta sa a ng ae ag na E a Tan Nia 41 Accessories and Spare Parts 0 0 cece ee ea eaaa eee 42 Device and Accessories saksa agan ag E eee eee eee nee 42 Device Variations seese o tisi ee eka thee ce eee eee ee ee eee als 43 Available Pump Heads 0 0 cee eee eee eee eee 43 Disposal 5 55 aini saga aroi Poa Hea Se Hane nadie we ERG RS TE Saree ek 43 Legal information sosse ee acs sca eis eis ach at saa va toa er eee ese NE a a le ayers 44 Warranty Conditions saie 0 0 aa Ka eee eee eee 44 Transport Damage rta 2044 5 A eee eee Seen oOo EOE ES 45 Abbreviations and Terminology 0 00 cece e eee eee eee 45 dex Si a ac eee ea a eam BREER A ag Talia Sale al to a Ta ga AB BG a a KA gg nebo a
19. a 46 Declaration of Conformity eee eee eee aaa 48 P 2 1S P 4 1S User Manual V6870 Version 2 0 Intended Use 5 Intended Use Note Only use the device for applications that fall within the range of the intended use Otherwise the protective and safety equipment of the device could fail Device Overview The HPLC pumps P 4 1S P 2 1S with pump heads can be used as feed pumps or dosing pumps in analytical or preparative applications Pumps transport sol vents or dissolved samples through the HPLC system Fig 2 P4 1S with 10 ml pump head Pump head Pressure sensor GROUND START IN ERROR INOUT ANALOG IN ANALOG OUT Electrostatic discharge Made in Germany Fig 3 Pump rear view P 2 1S P 4 15 User Manual V6870 Version 2 0 6 Intended Use Legend LAN port Opening of the fan Pin header for remote control CE mark Power connection bushing Serial number Hole for the ground connection RS 232 port External devices like computers fraction collectors etc can be connected in 3 different ways Connected to LAN within a network Connected to RS232 alternately to LAN connection Control with pin header Location In laboratories the device can be used in the following areas Biochemistry analyses Food analyses Pharmaceutical analyses Environmental analyses Chemical analyses Dosing applications Features Analytical pump head with a flow rate
20. ad with stainless steel inlay AHB40 10 ml pump head with ceramic inlay AHB32 10 ml pump head with Hastelloy C inlay AHB43 50 ml pump head with ceramic inlay AHC22 50 ml pump head with stainless steel inlay AHC20 Disposal Hand in old devices or disassembled old components at a certified waste facility where they will be disposed of properly AVV Marking in Germany According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactured by KNAUER are marked as waste electrical and elec tronic equipment 160214 WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German Elektroaltgerateregister EAR The number belongs to category 8 and 9 which among others comprise laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End users can send their old devices manufac P 2 1S P 4 1S User Manual V6870 Version 2 0 44 Legal Information tured by KNAUER back to the distributor the importer or the company free of charge but would be charged for the disposal Solvents and Other Operating Materials All solvents and other operating materials must be collected separately and dis posed of properly All wetted components of a device e g flow cells of detectors or pump heads and pressure sensors for pumps have to be flushed first with isopropanol and then with water before being m
21. aintained disassembled or disposed Legal Information Warranty Conditions The factory warranty for the device is valid for 12 months after the date of dis patch All warranty claims shall expire in the event that any unauthorized changes are made to the device During the warranty period any components with material or design related defects will be replaced or repaired by the manufacturer free of charge This warranty excludes the following accidental or willful damage damage or errors caused by third parties that are not contractually related to the manufacturer at the time the damage occurs wear parts fuses glass parts columns light sources cuvettes and other opti cal components damage caused by negligence or improper operation of the device and dam age caused by clogged capillary packaging and transport damage In the event of device malfunctions directly contact the manufacturer KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 111 Telefax 49 30 8015010 e mail info knauer net Internet www knauer net P 2 1S P 4 1S User Manual V6870 Version 2 0 Abbreviations and Terminology 45 Transport Damage The packaging of our devices provides the best possible protection against transport damage Check the devices for signs of transport damage In case you notice any damage contact the Technical Support and the forwarder company within three workdays
22. anual V6870 Version 2 0 10 Safety Protective gloves Lab coat What must be taken into account All safety instructions in the user manual The environmental installation and connection specifications in the user manual National and international regulations pertaining to laboratory work Original spare parts tools and solvents made or recommended by KNAUER Good Laboratory Practice GLP Accident prevention regulations published by the accident insurance compa nies for laboratory work Filtration of substances under analysis Use of inline filters Once they have been used never re use capillaries in other areas of the HPLC system Only use a given PEEK fitting for one specific port and never re use it for other ports Always install new PEEK fittings on each separate port Follow KNAUER or manufacturer s instructions on caring for the columns More safety relevant information is listed in alphabetical order in the following table Explanations flammability Organic solvents are highly flammable Since capillaries can detach from their screw fittings and allow solvent to escape it is prohibited to have any open flames near the analytical sys tem solvent tray Risk of electrical shock or short circuit if liquids get into the device s interior For this reason place all bottles in a solvent tray solvent lines Install capillaries and tubing in such a way that liquids cannot get into the interi
23. art of the IP address If more than one HPLC system is connected to the same LAN and you plan on controlling them separately you can use different ports to avoid interference Therefore the port number for each device must be changed and this same number must be entered into the device configuration of the chroma tography software We recommend to use the same port number for all devices in the same system Note The port is set to 10001 at the factory You must use the same numbers in the device configuration of the chromatography software as in the device other wise the connection fails Procedure 1 Find out port number and change it on the device 2 Enter the port number in the chromatography software Result The connection is established Ground CAUTION Electronic hazard when using an identically constructed power adapter from another manufacturer Before establishing a power connection call the Techni cal Support The ground connection for the pump has a designated hole with a thread M3 on the back of the device If the supplied power adapter is used then the ground connection remains unused P 2 1S P 4 1S User Manual V6870 Version 2 0 22 Operation Please contact the technical support of KNAUER if the pump along with other devices should be connected to the power supply with a 6 prong power adapter a pump needs to be grounded exclusively Operation Switching on the Pump CAUTION Damage to t
24. ct the pump using a LAN or RS 232 3 Enter STARTLEVEL 1 Starting Directly after Connecting to Power Supply By default the pump is stopped and started using the start stop button The STARTMODE setting allows you to start the device right after connecting it to power supply P 2 1S P 4 1S User Manual V6870 Version 2 0 32 Functionality Tests STARTMODE 0 default setting RS 232 LAN The pump does not start operat ing right after being connected to power supply STARTMODE 1 default setting Analog The pump starts operating right after being connected to power supply Enter in the terminal program using RS 232 STARTMODE 1 so that the pump is in operation directly after being connected to the power supply Prerequisite The pump is connected to the power supply Procedure 1 Connect the pump to a suitable terminal program 2 Connect the pump using a LAN or RS 232 3 Enter STARTMODE 1 Switching Off the Pump If you want to switch off the pump for a longer term flush the pump head with isopropanol Functionality Tests Installation Qualification IQ The customer may request the Installation Qualification which is free of charge In case of a request the Technical Support of KNAUER or from a provider autho rized by KNAUER performs this functionality test during the installation The Installation Qualification is a standardized document that comes as part of the delivery and includes the following confirma
25. e for latest updates and changes Translation of the original German edition of this manual version 2 0 Last manual update 2015 07 03 Printed in Germany on environmentally friendly paper from sustainable forests AZURA are registered trademarks of KNAUER Wissenschaftliche Ger te GmbH See up to date manuals online www knauer net downloads HPLC SMB Osmometry KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Telefax 49 308015010 E Mail L KNAUER info knauer net Internet www knauer net KNAUER 2015 V6870 0 01 10 12 Westkreuz
26. g purged The change takes effect immediately 1 Prime liquid with the syringe 2 Hold down start stop key until flushing starts Control The pump can be operated in two ways Using the buttons at the device Using the software ClarityChrom or ChromGate Note Operator errors and clogged capillaries can cause high pres sure spikes The status display shows the flow rate and also the pressure for pumps with pressure sensor P 2 1S P 4 15 User Manual V6870 Version 2 0 24 Operation Fig 10 Status display Legend Pressure in 0 1 MPa Flow rate in ml min Keypad The keypad consists of 3 keys which allow monitoring the device or changing the settings Figure Funetion O O Press both keys simultaneously to scroll lt gt Ee Press any of the arrow keys to set values and to change settings Fig 11 Arrow keys Starting and stopping the pump Ol gt Flushing the pump Fig 12 Start stop key Setting the Flow Rate In case of the pump without pressure sensor the actual required flow rate is dependent on the resulting counter pressure The absolute deviation is depen dent on the compressibility and the viscosity of the used solvent and on the pump Therefore it must be determined individually for each pump The flow rate can be altered while the pump is in operation Practical Tip Hold down both arrow keys to expedite changing the values 1 Use the arrow keys to set a value
27. he pump head in case it runs dry Ensure that liquids runs through pump head and piston backflushing Procedure 1 Connect the power adapter to the power supply 2 Connect the pump with plug from the external power adapter 3 Switch on the power adapter 4 Wait until the pump has completed the self test Result After the device is switched on the display shows Pump and the Firmware ver sion The device performs a self test After all tests have been successfully com pleted the status of the pump with its current flow rate is displayed The pump is ready for operation Selecting the Pump Head Procedure Press both arrow keys simultane ously until the correct display HEAD HEAD appears 10m1 50m1 Let go of the arrow keys Using the arrow keys scroll until 10 ml 50 ml Result When setting is finished the status display appears Fig 9 Pump head selection Flushing the Pump The display shows vertical arrows while the pump is flushing P 2 1S P 4 1S User Manual V6870 Version 2 0 Operation 23 Legend Flushing symbol Pressure Flow rate Prerequisites Pump with pressure sensor Venting screw of the pressure sensor is open Syringe is connected to venting capillary Pump without pressure sensor Blind fitting in the outlet to the pressure sensor is removed Syringe is connected to the capillary on the pump head outlet Note The flow rate can be changed while bein
28. le input min 10 mA Low active After receiving a signal e g short circuit to ground from an external device an error message appears and the device stops ANALOG IN Flow rate is controlled through external control voltage 0 10 V ANALOG OUT Analog output signal for reproducing the measured sys tem pressure Connecting the Pin Header To control one device through another you use the multi pin connec tor To use remote control you have to connect cables to the terminal strip everything comes included with delivery The single ports are used to exchange control signals Prerequisites The device has been turned off The power plug has been pulled Tools Depressor tool CAUTION Short circuit hazard Turn off the device before connecting it to the multi pin con nector Pull the power plug CAUTION Electrostatic discharge can destroy the electronics Wear a protective bracelet against electrostatic discharge and ground P 2 1S P 4 1S User Manual V6870 Version 2 0 18 Unpacking and Setup Place the terminal strip ona suitable surface Push the depressor tool into the opening on the upper side Continue pushing the depressor tool down and lead the cable o into the front end of the termi nal strip Remove the depressor tool Check whether the cables are a 3 tightly attached eee Plug the terminal strip onto the multi pin connector Fig 7 termi
29. ment 9 Salts 8 see Power supply 14 Self test 22 Setting the Flow Rate 24 Setting the power consumption 25 Software 23 Local Area Network 27 solvent P 2 1S P 4 1S User Manual V6870 Version 2 0 Index flammability 10 line 10 self ignition point 11 toxicity 11 tray 10 Solvents 8 Spare Parts 9 Steuerung der Pumpe 23 Switch on 22 T Technical Data 45 Technical data 41 Technical support 13 36 test Installation Qualification 32 Operation Qualification 32 Torque 40 transport damage 45 Ww Warning instructions 9 warranty 44 P 2 1S P 4 15 User Manual V6870 Version 2 0 47 lh KNALIER Declaration of Conformity Producer KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Deutschland Model Type Pump 1 P 2 15 Product No EPG90 Reference 2 P4 1S Product No EPG20 The product complies with the following standards Machinery Machinery Directive 2006 42 EC IEC 60799 1998 EMC EMC Directive 2004 108 EC IEC 61000 3 2 2009 IEC 61326 10 2006 Disposal RoHS Directive 2011 65 EU WEEE Directive 2012 19 EU Safety Low Voltage Directive 2006 95 EC IEC 61010 1 2001 The product was tested with a typical configuration C The mark of conformity has been applied to the rear panel Date Berlin 01 10 2014 Alexanda Knauer CEO and owner Revision 02 Part of V6870 KNAUER Wissenschaftliche Gerate GmbH All rights reserved Technical data are subject to change wit hout notice Please check our websit
30. nal strip Next steps Finish the installation and perform the initial startup Controlling with a Computer in a Local Area Network LAN This section describes how to set up an HPLC system in a local area network LAN and how a network administrator can integrate this LAN into your com pany network The description applies to the operating system Windows and all conventional routers Note To set up a LAN we recommend to use a router That means the following steps are required Process 1 On the computer go to the control panel and check the LAN properties Hook up the router to the devices and the computer On the computer configure the router to set up the network Install the chromatography software from the data storage device Switch on the device and run the chromatography software aA BW N Configuring the LAN Settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address P 2 1S P 4 1S User Manual V6870 Version 2 0 Unpacking and Setup 19 Prerequisite ajn Windows power saving hibernation standby and screen saver must be deactived In case you use an USB to COM box the option Allow the computer to turn off ths device to save power in the devicemanager must be deactivated for all USB hosts Only for Windows 7 For the network adapter the option Allow the com puter to turn off this device to save power in the De
31. nts Isocratic HPLC pump Expandable to a binary high pres sure gradient system controlled by software System protection Pump with pressure sensor Prin and Pmax adjustable Imin and Imax adjustable Pump with pressure sensor Imin and Imax adjustable P 2 1S P 4 15 User Manual V6870 Version 2 0 42 Accessories and Spare Parts Communication Operation LAN RS 232 Analog Buttons on the device Supply frequency 50 60 Hz Active power con maximum 40 W sumption P20 Technical parame Temperature range 4 40 C 39 2 104 F ters 3 i below 90 non condensing General Power supply 100 240 V 50 60 Hz Dimensions Pump without pressure sensor 220 x 121 x 138 1 mm Pump with pressure sensor 121 x 138 1 x 227 8 mm width x height x depth Weight Pump without pressure sensor 2 3 kg Pump with pressure sensor 2 4 kg Height above sea maximum 2000 m level Accessories and Spare Parts For repeat orders of spare parts use the enclosed packing list Contact the Tech nical Support in case there are any questions on spare parts or accessories Device and Accessories Name Order number S Pump APG Accessories kit AZURA FZAO1 Accessories kit P 2 1S P 4 15 FPGA 6870 P 2 1S P 4 15 User Manual V6870 Version 2 0 Disposal 43 Device Variations Available Pump Heads Pump P 2 1S with 10 ml pump head stainless steel APG90EA Name Order namber 10 ml pump he
32. olPhosphate buffer solutions 0 5 M Toluol Dilute ammonia solution P 2 1S P 4 15 User Manual V6870 Version 2 0 Scope of Delivery 9 Suitable eluents Dilute acetic acid 10 50 at 25 C 77 0 F Dilute sodium hydroxide 1M Water 1 valid for the specified temperature range Scope of Delivery Note Only use original parts and accessories made by KNAUER or a company authorized by KNAUER Pump P 2 1S P 4 1S Power adapter 24 V Installation Qualification Document Accessories Kit Pump Accessories Kit AZURA Safety Professional Group The user manual addresses persons who are qualified as chemical laboratory technicians or have completed comparable vocational training The following knowledge is required Fundamental knowledge of liquid chromatography Knowledge regarding substances that are suitable only to a limited extent for use in liquid chromatography Knowledge regarding the health risks of chemicals Participation during an installation of a device or a training by the company KNAUER or an authorized company If you do not belong to this or a comparable professional group you may not perform the work described in this user manual under any circumstances In this case please contact your superior Safety Equipment When working with the device take measures according to lab regulations and wear protective clothing Safety glasses with side protection P 2 1S P 4 15 User M
33. or in case of a leak leaks Regularly check if any system components are leaking power cable Defective power cables are not to be used to connect the device and the power supply sys tem P 2 1S P 4 1S User Manual V6870 Version 2 0 Safety 11 Explanations self ignition point Only use eluents that have a self ignition point higher than 150 C under normal ambient conditions power strip If several devices are connected to one power strip always consider the maximum power consumption of each device power supply Only connect devices to voltage sources whose voltage equals the device s voltage Organic eluents are toxic above a certain con centration Ensure that work areas are always well ventilated Wear protective gloves and safety glasses when working on the device Where is use of the device prohibited Never use the system in potentially explosive atmospheres without appropriate protective equipment For further information contact the Technical Support of KNAUER Decommissioning the Device Securely At any time take the device completely out of operation by either switching off the power switch or by pulling the power plug Opening the Device The device may be opened by the KNAUER Technical Support or any company authorized by KNAUER only Definition of Personal and Material Damage Possible dangers related to the device are divided into personal and material damage in this user manual Category
34. s of 100 240 V Power cable Only the supplied power cable is to be used to connect the device to the mains supply Replace defective power cables only with accessories from KANUER Only use power cables with a permission for use from your country Power plug Make sure that the power plug on rear of the device is always accessible so that the device can be disconnected from the power supply Unpacking Store all packing material Retain included packing list carefully for repeat orders CAUTION Damage to the device by carrying or lifting it on protruding housing parts Lift the device on the side of the housing only 1 Setup the delivery so you are able to read the label Using the utility knife cut the adhesive tape Open the delivery 2 Remove the foam insert Take out the accessories kit and the manual 3 Open the accessories kit and take out all accessories In case any parts are missing contact the technical support Grip the device at its side panels and lift it out of the packaging 4 Remove the foam inserts from the device Pull off the antistatic bag if neces sary 5 Check for damages caused during transportation In case you notice any damage contact the technical support P 2 1S P 4 1S User Manual V6870 Version 2 0 Unpacking and Setup 15 6 Set up the device in its location 7 Check the scope of delivery 8 Remove the protective foil Result The device is complete and ready for use Next
35. steps Connect the device to power supply to prepare the initial startup Connecting the Eluent Line to the Pump Head Prerequisites The device has been switched off The power plug has been pulled Material Flangeless fitting CAUTION Damage to the pump head possible Remove the cap fittings from the inlet and outlet of the pump head prior to use Slide the flangeless fitting onto the tubing Insert the tubing into the free inlet on the bottom of the pump head Tighten the fitting by hand Fig 5 Solvent line on the pump head Next steps Integrate the pump into the HPLC flow system P 2 1S P 4 1S User Manual V6870 Version 2 0 16 Unpacking and Setup Control GROUND START IN ERROR INOUT ANALOG IN ANALOG OUT Electrostatic discharge Fig 6 Pump rear view Legend LAN port CE mark Pin header for remote control Opening of the fan Power connection bushing Serial number Warning 1 RS 232 port Hole for the ground connection Pin Header for Remote Control Contact data Explanation GROUND Reference point of the voltage at the signal inputs START IN TTL compatible input min 10 mA Low active After receiving a signal from an external device the device starts If controlled with software an electronic trigger is send through the LAN P 2 1S P 4 15 User Manual V6870 Version 2 0 Unpacking and Setup 17 Contact data Explanation ERROR IN OUT TTL compatib
36. ter and the devices can be established Check after each step if the problem is solved If the problem cannot be located call the Technical Support 1 Check the status of the LAN connection in the Windows task bar fal Connected kal Connection not established If no connection was established test the following Is the router switched on Is the patch cable connected correctly to the router and the computer P 2 1S P 4 15 User Manual V6870 Version 2 0 34 Troubleshooting 2 Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connections between devices and router cor rect Are the cables plugged in tightly 4 If the router is integrated into a company network pull out the patch cable from the WAN port Can the devices communicate with the computer even though the router is disconnected from the company net work 5 In case you own a Control Unit check the settings in the menu Setup gt Network Is LAN DHCP set for controlling Did the device receive an IP address 6 Turn off all devices router and computer Firstly turn on the router and secondly turn on the devices and the com puter Has this been successful 7 Replace the patch cable to the device with that no connec tion could
37. tion of flawless condition at delivery check if the delivery is complete certification on the functionality of the device Operation Qualification OQ The Operation Qualification includes an extensive functionality test and must be purchased from the manufacturer Contact the KNAUER Sales Department to request an offer The Operation Qualification is a standardized KNAUER docu ment and includes the following definition of customer requirements and acceptance terms documentation on device specifications device functionality check at installation site P 2 1S P 4 1S User Manual V6870 Version 2 0 Troubleshooting 33 Test Intervals To make sure that the device operates within the specified range you should test the device using the Operation Qualification at following intervals Average Useful Life 1 to 5 days week Every 6 months More than 5 days week or 24 hours day Every 3 months Operation with buffer solutions or other salt Every 3 months solutions Execution The test can be carried out either by the Technical Support of KNAUER or from a provider authorized by KNAUER Troubleshooting First measures for troubleshooting Check all screw fittings Check whether air has gotten into the supply lines Check device for leaks Further measures Check errors against error list Contact the technical support of KNAUER LAN Go through the following steps in case no connection between the compu
38. trasonic bath for at least 10 minutes Installing the Check Valves Insert the check valves in the direction of the flow The notch of the check valve points downward Insert the NP check valves in the direction of the flow The arrow on the NP check valve points upward Prerequisite The check valves have dried CAUTION Damage to components caused by overtightened fittings Note the torque of the fittings For stainless steel fittings 5 Nm For PEEK fittings maximum 1 Nm or finger tight and an addi tional quarter of a turn with appropriate pliers Insert the check valves in such a way that the notch of the check valve points downward Screw in inlet and outlet fittings and tighten them with a torque wrench and the respective torque Fig 26 Check valve Next steps Re install the pump head P 2 1S P 4 15 User Manual V6870 Version 2 0 Technical Data 41 Technical Data Solvent delivery Pump type Dual piston pump with main and auxiliary piston Flow rate range 10 ml pump head 0 001 10 ml min 50 ml pump head 0 01 50 ml min Maximum pressure 10 ml pump head 40 MPa to 10 ml min Imax 70 50 ml pump head 15 MPa to 50 ml min Imax 80 Flow rate accuracy 1 measured at 5 50 of flow range using ethanol water 10 90 For pumps without a pressure sen sor dependent on pressure Flow rate precision Relative standard deviation RSD lt 0 5 1 ml min Gradie
39. ushing the Pistons When you flush the pistons regularly the service life of the seals and pistons increases While flushing contaminants are washed from the rear piston area The following solvents are recommended for flushing the columns Water Mixture of 80 water and 20 ethanol lsopropanol Connect the outlet to the waste container with a hose Connect the inlet to the syringe with a hose Fill up flushing solution with the syringe through the pump head until there are no more air bubbles running through the waste bottle Afterwards remove the hoses and connect inlet and outlet with a hose Fig 18 Flushing rear piston area Software To be able to control the pump using chromatography software the computer must be connected to the device either with a RS232 cable or a LAN cable Via Local Area Network LAN A device connected to a LAN is recognized by the software and automatically receives an IP address because it is set to Dynamic Host Configuration Protocol DHCP at the factory Prerequisite Device has been connected to LAN Status display is active P 2 1S P 4 1S User Manual V6870 Version 2 0 28 Operation Press both arrow keys simultane ously until the correct display appears LAN Let go of the arrow keys Using the arrow keys scroll until Fig 19 Display control LAN Results When setting is finished the status display appears Via RS 232 Port
40. vice Manager must be deactivated Procedure In Windows 7 choose Start Control Panel Network and Sharing Center Double click on LAN Connection Click on the button Properties Select Internet Protocol version 4 TCP IPv4 Click on the button Properties Check the settings in the tab General The correct settings for the DHCP cli ent are a Obtain IP address automatically b Obtain DNS server address automatically 7 Click on the button OK NU BW N Connecting the Cables A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network WAN e g a company network or the Inter net In contrast the LAN ports serve to set up a network from devices and a computer To avoid interference we recommend operating the HPLC system separately from the company network You will find patch cables for each device and the router in the accessories kit To connect the router to a WAN an additional patch cable is required which is not supplied within the scope of delivery Modules WAN port Router Workstation LAN ports P 2 1S P 4 1S User Manual V6870 Version 2 0 20 Unpacking and Setup Fig 8 Cabling system LAN Prerequisite The computer has been switched off There is a patch cable for each device and the computer Procedure 1 Use the patch cable to connect the router and the computer Repeat this step to connect all devices
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