* Bio-Link – BLX user manual
Contents
1. A WARNING A UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 3 8 Indice 1 16 11 09 Setting up and descriptions Operating instructions Place the BIO LINK on a level working surface Be sure there is enough place in front of the door to open it easily Plug your BIO LINK into a properly grounded electrical outlet Put the ON OFF switch to the ON position R The display will show the value in Joules of the last Ga irradiation the green light is on for the first start up the L E D will display the factory setting Open the door and place your sample requiring exposure in the middle of the chamber Close the door Joules Preset Minutes I Different irradiation settings are available A Irradiation in Joules with manual setting B Irradiation in Time with manual setting C Irradiation in Joules by preset setting D Irradiation in Time by preset setting gt A Irradiation in Joules with manual setting when activated the display shows the value in Joules of the last irradiation the green light is on fi 0 120 J cm if this setting suits you press START the display will begin to countdown at the end of the ir2. Bio Link BLX user manual 1 8 Indice I 16 11 09 The BIO LINK crosslinker BLX is a complete microprocessor controlled UV irradiation system mainly dedicated to the linking of nucleic acid to membranes and elimination of PCR contamination Its innovative design ensures unique features Microprocessor controlled The programmable microprocessor constantly monitor the UV light emission The irradiation stops automatically when the energy received matches the programmed energy Reproducibility Thanks to its UV sensors irradiation cycles are perfectly reproducible regardless of intensity fluctuation of the UV source Just programme your energy and BIO LINK delivers it Durability Bio Link combines the latest technology with a very high quality of components UV exposure chamber in stainless steel protective quartz disk on the UV sensor cell highly resistant tactile membrane keypad Ease of use The readout display and the large number of presets in either energy unit Joules cm or time unit seconds makes the BIO LINK a very simple instrument to use while very powerful Consistent measure The UV light intensity is captured in a well of light positioned above the irradiation chamber The UV cell measure is then collected from all the UV tubes and not jut one This also protect the UV cell from any dirt which can enter the chamber Specifications Microprocessor control Large L E D readout Precise irrad
3. NEVER USE SOLVENTS OR ABRASIVE PADS Changing Tubes Obtain from your distributor e 5 x 8 Watt tubes in the same wavelength and 5 Starters as your unit see table of spare parts Open the door of your BIO LINK Remove the old tubes A quarter turn will release the tubes Replace with the new tubes ATTENTION Check the unit functions correctly only after completely reassembling To reassemble follow the above instructions in reverse order Changing the Wavelength You have chosen a BIO LINK equipped with one of the 3 available wavelengths It is always possible to change this wavelength and use your BIO LINK for other applications as follows Before any servicing unplug the unit Open the door of your BIO LINK Remove the 5 tubes a quarter turn will release them Replace with the 5 tubes in the new wavelength Close the door of your BIO LINK Plug the unit Press ENTER during 5 seconds and keep holding it put the ON OFF switch to the ON position The BIO LINK will display the previous wavelength example L 254 Release the ENTER the display will flash To select the new wavelength display one after another the 3 possibilities e L 254 L 312 L 365 with the cursor Y key 8 A key 2 Once the wavelength corresponding to the tubes is displayed press ENTER A WARNING UV radiation can be dangerous for unprotected ey
4. e UV radiation Protect the eyes and skin from the UV radiation gt Climatic conditions Altitude lt 2000 meters Operating humidity 20 to 80 no condensation allowed Operating temperature 5 to 33 C Type of fuse Type FST Time lag T 5x20 gt Power See the power values for each item in the spare parts and characteristics board on the page 6 Indice I 16 11 09 2 8 Characteristics and dimensions Operating Instructions e EXTERNAL DIMENSIONS height 30 5cm depth 36cm width 35cm e INTERNAL CHAMBER DIMENSIONS height 14 5cm Depth 33cm width 26cm e WEIGHT 10 kg e ULTRAVIOLET WAVELENGTH 254nm 312nm or 365nm e ULTRAVIOLET SOURCE 5 x 8 watt tubes e POWER 80 W e MULTIPLE SET FUNCTIONS 9 preset UV Energy exposures 9 preset UV exposure Time manual setting of UV Energy exposure Min 0 025 Joules Max 99 99 Joules manual setting of UV exposure Time Min 10 Seconds Max 599 Minutes e ENERGY DISPLAY 2 measurement ranges from 0 025 to 9 999 Joules from 00 25 to 99 99 Joules e EXPOSURE TIME DISPLAY 2 measurement ranges from 00 10 to 99 59 Minutes Seconds from 000 1 to 599 5 Minutes Seconds Touch panel Membrane keypad ON OFF Switch Outer Casing p ri 5 ane J 7 P 7 p i J p Viewing Hinges window Handle
5. ertificate of decontamination The Buyer must bear all costs and risks incurred during the transportation of the goods from their collection at Vilber Lourmat warehouse In the case VILBER LOURMAT incorporates some devices or equipment from another supplier in the manufacture of its products the extent and the duration of the warranty will be those conceded by the suppliers or sellers Manufacturer cannot be held responsible for any loss bodily injury or material accident incurred by any failure of this supply whatever the origin of this failure may be The responsibility of Manufacturer is strictly limited to its staff and to its own supplies In the case of dispute only the commercial court of Meaux France shall be competent even in third party claims proceedings or when there are several co defendants NOTE Vilber Lourmat is not responsible for any injury or damage caused by use of this instrument for purposes other than those for which it is intended or by modifications of the instrument not performed by Vilber Lourmat Decontamination collection and elimination of waste for France only The buyer ensures and finances the decontamination the collection and the disposal of waste electrical and electronic equipment WEEE under the conditions provided in the Articles 21 and 22 of the Decree No 2005 829 dated of 20 July 2005 In France for tubes recycling contact the company Recylum www recylum com Improper disposal may be har
6. es and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 gt Warranty Indice 1 16 11 09 718 bes and filters are warranted against faulty construction or defective 3 date of supply Our products are not warranted for damage due to i Tett on an led ian Vilber Lourmat or an authorised agent ples an Vilber Lourmat J in any way Modification or alteration of this instrument will 3 Create a potential safety hazard The Compact Flash the tubes and the filters are not covered by our warranty The use of consumable products or non original spare parts not recommended by our service department is at the user s own risk and therefore automatically invalidates the warranty Tubes filters batteries and consumable products are not included in the warranty We reserve the right to decide where the faulty goods will be repaired in our workshop or elsewhere and whether or not the faulty part is to be replaced all other freight charges incurred being at the cost of the purchaser Returned goods will not be accepted for repair unless previous written authorisation is obtained from our service department A request for authorisation must be accompanied by an itemised list of products model numbers and the corresponding invoice numbers under which they were originally shipped All returned goods should have a c
7. etc The BIO LINK BLX is also available in 312nm and 365nm References Khandjian E W Biotechnology 5 February 1987 Khandjian E W Mol Biol Rep 11 107 115 1986 Church GM amp Gilbert W Proc Nath Acad Sci 81 1991 1995 1984 Vollrath D amp Davis R W Nucl Acids Res 15 7865 7876 1987 Whittaker P A Southern E M Gene 41 129 134 1986 J Sambrook E F Fritsch amp Maniatis T Molecular Cloning A Laboratory Manual Cold Spring Habor Laboratory L OV Is Ne es Wilson K H Biotechniques Vol 2 n 2 1992 UV Absorption Complicates OCR Decontamination gt Spare parts Tubes Starters Fuse 5 x 20 Ref Article Qty Ref Wavelength W ST 151 230 V 100 V Power FG7 P 100V 240 V 115 V BLX 254 5 T 8 C 254nm SW 5 2A 2A 80 W BLX 365 5 T 8 L 365nm SW 5 2A 2A 80 W BLX 312 5 T 8 M 312nm SW 5 2A 2A 80 W WARNING UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 6 8 Indice I 16 11 09 Maintenance Cleaninc Before cleaning unplug your unit Clean the outside of the BIO LINK with a slightly wet sponge and wipe with a soft cloth The viewing window must be cleaned with a soft cloth and alcohol The stainless steel chamber must be cleaned with a soft cloth and alcohol ATTENTION
8. etting on the touch panel membrane keypad in minutes and tenth of minute then press ENTER press PRESET the blue light PRESET is on press ENTER the display will show P Select a code number from 1 to 9 for your setting on the touch panel membrane keypad fi 5 Your setting is stored under code 5 NOTE In PRESET mode with blue light PRESET on if no value is set within 20 seconds the unit will return to initial Joules or Time mode to start an irradiation using a preset Time setting press PROGRAM to select Time mode the blue light MINUTES will be on press PRESET the blue light PRESET is on and enter on the touch panel membrane keypad the code number of the required preset setting fi 5 the display will show the value of the preprogrammed code 5 press START the display will begin to countdown at the end of the irradiation the unit will automatically stop the setting of the programmed code 5 is stored and will be displayed at each start up or each change of setting mode to delete a displayed setting press STOP the display will show 000 0 CAUTION In Joules or Time mode it is possible to preset a new setting under a code already used The old value will be deleted for the new one at the end of the irradiation cycle open the door and remove your sample NOTE During an irradiation cycle you can stop by pressing STOP The irradiation is stopped and t
9. he display will show the total value of the setting In that case it is not possible to restart the irradiation Opening the door during an exposure cycle will immediately stop the irradiation thus protecting the user from the Ultraviolet radiation WARNING A UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 Indice I 16 11 09 5 8 Applications The BIO LINK BLX 254 is a polyvalent UV 254nm irradiating system mainly dedicated for the following applications in the laboratory e Crosslinking of DNA and RNA by covalently binding nucleic acids to nitrocellulose or nylon membranes for Southern Northern dot or slot blots procedures 1 2 3 To operate the BIO LINK BLX you preset the UV Energy required in Joules cm 10 preset UV Energy exposures and manual setting A factory preset UV dosage of 0 120 Joules cm has been stored preset 0 for the linking of nucleic acids This setting has been found to be the optimal dosage for DNA attachment and hybridization signal sensitivity Nicking of Ethidium Bromide stained DNA in agarose gel 4 e Partial restriction endonuclease digestion by formation of thymine dimers 5 RecA mutation screening 6 Elimination of PCR contamination 7 The BIO LINK BLX is useful for other applications such as UV sterilisation UV curing of polymers
10. iation in either energy Joules cm or Protective quartz disk on the UV sensor cell time seconds Spacious UV exposure chamber in stainless steel Preset programme for dosage of 0 120 J cm to Safety interlock door with UV blocking observation optimised nucleic acid immobilisation window 9 preset programmes for UV energy exposure Automatic restart with no loss of information if 9 preset programmes for time exposure breaking off of circuit Manual setting of UV energy or time exposure Dual safety fuses Storage of the last UV setting UV wavelength interchangeability Tactile membrane keypad All our BIO LINK come equipped with the following Touch panel membrane keypad Internal safety interlock Large led readout UV blocking viewing window No loss of information if circuit interrupted Large stainless steel internal UV exposure chamber Storage of the last UV setting energy and time Removable power cable Warning On the safety a This unit must be connected to a wall outlet having protective earth terminal Connecting to ground is an obligatory protection b To prevent fire or shock hazard do not expose the unit to rain or moisture c Unplug the unit from the wall outlet if you do not use it for a long time Disconnect the power cord by grasping the plug Never pull the cord itself d Never obstruct the air admission grids of the unit A This symbol is warning against a possibl
11. mful to the environment and human health Declaration of conformity The materials complies with the requirements of the EC Directive 89 336 EEC 73 23 EEC and EN 61010 1 C electro magnetic compatibility and low voltage The electro magnetic susceptibility has been chosen at a level that gains proper operation in residential areas on business and light industrial premises and on small scale enterprises inside as well as outside of the buildings All places of operation are characterized by their connection to the public low voltage power supply system 8 8 Indice 1 16 11 09
12. n press PRESET the blue light PRESET is on and enter on the touch panel membrane keypad the code number of the required preset setting fi 3 the display will show the value of the preprogrammed code 3 press START the display will begin to countdown at the end of the irradiation the unit will automatically stop the setting of the programmed code 3 is stored and will be displayed at each start up or each change of setting mode to delete a displayed setting press STOP the display will show 0 000 CAUTION In Joules or Time mode it is possible to preset a new setting under a code already used The old value will be deleted for the new one at the end of the irradiation cycle open the door and remove your sample NOTE During an irradiation cycle you can stop by pressing STOP The irradiation is stopped and the display will show the total value of the setting In that case it is not possible to restart the irradiation Opening the door during an exposure cycle will immediately stop the irradiation thus protecting the user from the Ultraviolet radiation Re closing the door will restart the irradiation where it stopped D irradiation in Time by preset setting in Time mode your BIO LINK can accept 9 preset settings These preset settings are numbered from 1 to 9 to enter a preset setting press PROGRAM to select Time mode the blue light MINUTES is on enter the value of the s
13. radiation the unit will automatically stop if the displayed value does not suit you enter directly on the touch panel membrane keypad another value then press ENTER the setting is stored only if an irradiation cycle is started to start the irradiation press START the display will begin to countdown this new setting is now stored and will be displayed at each start up or each change of setting mode gt B Irradiation in Time with manual setting when activated the display shows the value of the last irradiation in Joules mode the blue light JOULES is on press PROGRAM to change to Time mode blue light MINUTES is on the display shows the value of the last irradiation in minutes and tenth of minute if this setting suits you press START the display will begin to countdown at the end of the irradiation the unit will automatically stop You can change the UV exposure time directly by pressing the touch panel membrane keypad note in minutes and tenth of minute then press ENTER the setting is stored only if an irradiation cycle is started to start the irradiation press START the display will begin to countdown at the end of the irradiation the BIO LINK will automatically stop and this new setting is stored and will be displayed at each return in Time mode C Irradiation in Joules by preset setting in Joules mode your BIO LINK can receive 10 p
14. reset settings the code 0 being factory set at 0 120 J cm this setting is fixed and cannot be deleted the 9 others are available numbered from 1 to 9 to enter a preset setting select the Joules mode by pressing PROGRAM the green light is on enter the value of the setting on the touch panel membrane keypad then press ENTER A WARNING A UV radiation can be dangerous for unprotected eyes and skin therefore we recommend the user to wear UV protective goggles LP 70 or face shield MP 80 or MP 800 4 8 Indice 1 16 11 09 in Joules mode your BIO LINK can receive 10 preset settings the code 0 being factory set at 0 120 J cm this setting is fixed and cannot be deleted the 9 others are available numbered from 1 to 9 to enter a preset setting select the Joules mode by pressing PROGRAM the green light is on enter the value of the setting on the touch panel membrane keypad then press ENTER press PRESET the blue light PRESET is on press ENTER the display will show P Select a code number from 1 to 9 for your setting on the touch panel membrane keypad fi 3 Your setting is stored under code 3 NOTE In PRESET mode with blue light PRESET on if no value is set within 20 seconds the unit will return to initial Joules or Time mode to start an irradiation using a Joules preset setting press PROGRAM to select Joules mode the blue light JOULES will be o
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