Leica_DM750M_Manual_EN
Contents
1. 24 Installing the Condenser Condenser 1 Move the specimen stage upwards as far as 3 Unscrew the two setscrews or wingscrews H The Leica DM750 M is equipped with it will go using the coarse focusing knob for Koehler stands on the condenser an open holder for the specimen stage holder condenser in other words the condenser must be installed 2 Move the condenser holder into the lowest position using the condenser focusing The holder of the condenser is open knob Leica DM750 M Manual 25 Installing the condenser continued 4 Push the condenser under the specimen 5 Move the condenser into the highest posi Stage into the holder by aligning the loca tion using the condenser focusing knob ting pin on the bottom of the condenser with the slot on the rear side of the fork 6 Tighten the two setscrews Koehler stand wingscrews so that the upper lens of the condenser is centered under the objec 0 90 1 25 OIL S1 tive in working position and the specimen 10x 20x 40x 100x pharrell Min stage condenser is thus roughly centered How to center the condenser more Li precisely is described in the section entitled Complete Condenser Centering on page 28 Leica DM750 M Manual 26 Switching on the Microscope Precautionary measures The light of the Leica DM750 M can be A very bright Therefore do not look into the eyepieces until after you have switched on the illumination Le2. ler illumination center the condenser as described below knob on the left side of the stage mount 1 Adaptthe Koehler field diaphragm to the base of the microscope so that the illuminated field diaphragm is in the field of view when you look through the eyepieces Condenser focusing knob Illuminated field diaphragm is focused Close the Koehler field diaphragm Closed Koehler field diaphragm Leica DM750 M Manual 36 Koehler Configuration cont 3 Turn the condenser centering wingscrews simultaneously to center 4 Open the field diaphragm until the diaphragm leaves are just outside the image of the field diaphragm of the field of view Leica DM750 M Manual 37 Automatic Shutoff of Illumination The illumination of the Leica DM750 M H is shut off automatically if the user does not make any modifications for two hours This setting can be changed If the incident light illumination is disconnected from the power supply and then reconnected automatic shutoff is always activated factory setting Leica DM750 M Deactivating delayed shutoff 1 Switch the incident light axis off 2 Hold the button pressed while swit ching on the incident light axis OOo o o After the delayed shutoff is deactivated the LED flashes three times slowly and then stays steady Manual Activating delayed shutoff 1 Switch the incident light axis off 2 Hold the G button pressed while swit
3. Leica DM750 M Manual MICROSYSTEMS Table of Contents Safety instructions 3 Conversion and Use with Transmitted Light Safety Notes 4 Optional Koehler Illumination 24 Instructions on use 5 Installing the Condenser 25 Instructions on use continued 6 Installing the condenser continued 26 Switching on the Microscope 27 Complete Condenser Centering 28 Assembling the Incident Light Axis 8 Complete Condenser Centering cont 29 Assembling the Viewing Tubes 9 Using the Condenser 30 Leica EZ Viewing Tube with Integrated Eyepieces 10 Preparation for Viewing a Specimen Slide 31 Leica EZ Viewing Tube integrated eyepieces cont 11 Focusing 32 Leica Standard Tube with Separate Eyepieces 12 ViewingTube Adjustment 33 Eyecups 13 Viewing Tube Adjustment cont 34 Installing Objectives 14 Viewing Tube Adjustment cont 35 Conversion to Incident Light Polarization 15 Koehler Configuration 36 Koehler Configuration cont 37 eee Automatic Shutoff of Illumination 38 Switching on the Microscope Modifying the Aperture Diaphragm 7 Care and Repair Instructions Illumination Scenarios 19 Care Instructions 40 Illumination Scenarios continued 20 Accessories Maintenance and Repair 41 Using Specimen Holders 21 Automatic Shutoff of Illumination imeenspecifications and Dimensions Electrical Data and Ambient Conditions 43 Dimensions in m
4. tening the wingscrew again The eyepieces are integrated into the Leica EZ Viewing Tube and preset there fore there is no need to adjust or install the eyepieces Continue with the Eyecups section on page 13 Leica DM750 M Manual 11 Leica Standard Tube with Separate Eyepieces 1 Insert the eyepieces into the tubes 2 Secure the eyepieces in the tubes by tighte ning the silver screws at the bottom Standard tubes tubes do not include eyepieces yet The standard tube includes a rotatable dovetail Therefore you can now rotate the standard viewing tube freely in any orien tation The eyepieces can still be rotated but no longer detached from the tubes Leica DM750 M Manual 12 Eyecups If you wear eyeglasses for microscope H viewing keep the rubber eyeguards folded down If you do not wear eyeglasses you may find it useful to unfold the rubber eyegu ards in order to help block out ambient room light Leica DM750 M Manual If you have purchased a standard micro Li scope configuration the objectives are pre installed on the objective nosepiece and the specimen stage condenser is installed onto the stand In this case continue with the Use section starting on page 17 If you have purchased your Leica DM750 M in individual components and not according to the standard configuration continue with the Installing Objectives section on page 14 Installing Objectives Insta
5. 03 Transport shock unpacked 100 g 6 ms Transport shock packed 800 mm free fall Transport vibrations unpacked 5 200 Hz 1 5g Electrical data Air pressure during use and storage 500 1060 mbar Input 100 240 V 50 60 Hz 5 W 3 W LED i Humidity during use and storage 20 90 Installation Category Il Overvoltage Category Pollution degree 2 Leica DM750 M Manual 43 Dimensions in mm 382
6. atic Shutoff of Illumination The illumination of the Leica DM750 M H is shut off automatically if the user does not make any modifications for two hours This setting can be changed If the incident light illumination is disconnected from the power supply and then reconnected automatic shutoff is always activated factory setting Leica DM750 M Deactivating delayed shutoff 1 Switch the incident light axis off 2 Hold the button pressed while swit ching on the incident light axis OOo o o After the delayed shutoff is deactivated the LED flashes three times slowly and then stays steady Manual Activating delayed shutoff 1 Switch the incident light axis off 2 Hold the G button pressed while swit ching on the incident light axis o o o o After the delayed shutoff is activated the LED flashes three times quickly and then stays steady Conversion and Use with Transmitted Light Optional Koehler Illumination Transmitted illumination For the Leica DM750 M there are two Li condensers available for optimum trans mitted illumination Determine which type of illumination is used for your instrument since this will be important later Leica DM750 M Type 1 Standard condenser Adjustable condenser centering with tool kept at the ready Manual Type 2 Koehler condenser Adjustable condenser centering with wingscrews and adjustable Koehler field diaphragm 4 A 4 i i rA
7. b while looking through only one of the eyepieces Cover your other eye A If you are using one focusable and one non focusable eyepiece look through the non focusable eyepiece Manual 3 Now look with the other eye just through the other eyepiece focusing eyepiece Bring the specimen into focus using the focus function of the eyepiece When doing so do not change the height of the specimen stage 4 Grip the knurled ring on the focusing eyepiece with one hand and rotate the top of the eyepiece with the other hand until the specimen is in focus for this eye and this focusing eyepiece This corrects for any vision differences between your right eye and left eye Viewing Tube Adjustment cont 5 Now switch to an objective with a high magnification level and bring the micro scope into focus while looking through the eyepieces with both eyes The depth of field is lower for higher magnification levels Thus you will notice that if you switch to low magnification after bringing the microscope into focus with great magnification a minor adjustment of the fine focus may be required e If your DM750 M is equipped with a Koeh ler configuration continue with the next section entitled Koehler Configuration on page 36 Leica DM750 M Manual 35 Koehler Configuration i If your Leica DM750 M is equipped with a field diaphragm for Koeh 2 Focus the illuminated field diaphragm using the condenser focusing
8. ce and Repair Accessories Only the following accessories may be used with the Leica DM750 M e The Leica Microsystems accessories descri bed in this manual e Other accessories provided that these have been expressly approved for use by Leica Microsystems with regard to safety tech nology Leica DM750 M Maintenance e The Leica DM750 M is basically mainte nance free To ensure that it always opera tes safely and reliably we recommend that you take the precaution of contacting the responsible service organization H You can arrange for periodic inspections or if appropriate conclude a mainte nance contract with them Itis advisable to enter a service agreement with Leica Service e For maintenance and repair only OEM Spare parts may be used Manual Service address In case of problems please contact us as follows Leica Microsystems Schweiz AG Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Phone 41 71 726 33 28 41 Specifications and Dimensions Electrical Data and Ambient Conditions Fuse Replacement Environment Unplug the instrument before changing any fuses The Temperature for use 10 C 40 C Leica DM750 M includes two fuses that are located behind Storage temperature 20 C 52 C the power cable compartment 50 F 104 F Only use the following fuse types 5x20 mm 1 A 250V KOMPATE PO O rO fast acting fuse 13RFAG300
9. ching on the incident light axis o o o o After the delayed shutoff is activated the LED flashes three times quickly and then stays steady Care and Repair Instructions Care Instructions General instructions e Protect the Leica DM750 M from moisture vapors acids alkalis and corrosive substan ces Do not store chemicals in the vicinity Protect the Leica DM750 M from oil and grease Never grease or oil mechanical parts or sliding surfaces Follow the instructions of the disinfectant manufacturer It is advisable to enter a service agreement with the Leica Service Leica DM750 M Cleaning coated parts and plastic parts Dust or dirt particles should be removed with a soft brush or a lint free cotton cloth Remove coarse debris with a moistened disposable cloth Clinging dirt can be removed using petro leum ether or alcohol Acetone xylene or nitro containing thin ners must NOT be used Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abra ded particles Manual Cleaning glass surfaces Remove dust using a dry and grease free brush made from hair by blowing with bellows or by using a vacuum Remove dirt using a clean cloth that has been moistened with distilled water Remove tough dirt using alcohol chloro form or petroleum ether 40 Accessories Maintenan
10. ectrically operated accessories are construc ted based on the state of the art of technology and are provided with an EC Declaration of Conformity Contact address Leica Microsystems Schweiz AG Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Instructions on use Intended use The DM series microscopes by Leica are opti cal instruments for improving the visibility of objects through magnification and illumina tion They are used for observing and docu menting Place of use Use the instrument in enclosed dust free rooms at 10 C to 40 C only Protect it from oil chemicals and extreme humidity It must be at least 10 cm from the wall and away from flammable subs tances Leica DM750 M Interference Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Use in clean rooms Leica DM series microscopes can be used in clean rooms without any problems Manual Handling electrical components Never install any other plug NEMA 5 15P or unscrew any mechanical components unless expressly instructed to do so in the instructions The microscope must be connected to a grounded socket Always position the microscope so that A you can disconnect it from the power Su
11. ica DM750 M Connecting and switching on the micro scope 1 Plug the power cable of the microscope into a corresponding grounded socket 2 Reduce the brightness to the minimum Manual 3 Switch on the microscope using the switch at the bottom right of the microscope stand 4 Look through the eyepieces and adjust the brightness to the desired level Complete Condenser Centering Complete Condenser Centering 2 Write an X on a piece of paper in the size 1 Open the condenser aperture by rotating of a business card and place it onto the the knurled ring on the condenser to the light output of the microscope stand in right such a way that the X is centered over the illumination Make sure that the condenser is in the 3 Look through the eyepieces and center the highest position X in the field of view by tightening the setscrews Leica DM750 M with Koehler Centered illumination tighten the knurled screws Leica DM750 M Manual 28 Complete Condenser Centering cont 4 ForaLeicaDM750M with standard conden ser without Koehler field diaphragm you can now lock the condenser into its posi tion by tightening the setscrews on both sides of the condenser fork using the tool included in the delivery package Setscrew Leica DM750 M For a Leica DM750 M with Koehler field diaphragm center and focus the conden ser more precisely The procedure is described in the Koehler Configurat
12. ight field Illumination Scenarios continued Adding light fields Press a button only briefly to switch an additio nal light field on or off Switching to bright field Press the middle button briefly to toggle between bright field illumination and the previously selected oblique light configuration O 09 O O 4 09 O Or Switching between bright field illumination and the most recent setting Any arrangement Leica DM750 M Manual Automatically calling up oblique light illumination scenarios Hold the middle button pressed longer than two seconds as long as you do not release the button the next light field is activated every two seconds via ol 4 a Switching automatically between the light fields Using Specimen Holders Additional specimen holders Inserting the specimen holder 4 Set the specimen holder into the stage Specimen holders are available in two 1 Unscrew the ring from the specimen holder aperture and push it to the rear as far as it different versions with a diameter of 25 will go mm and 30 mm 2 Insert the specimen e Order number for 25 mm version 13 613 3 Screw the ring onto the specimen holder 167 e Order number for 30 mm version 13 613 168 5 Position the specimen by moving the stage and looking through the eyepieces simulta neously The maximum height of the specimen is 30 mm Leica DM750 M Manual 21 Autom
13. ion section on page 36 Manual Using the Condenser Using the Condenser The condenser is furnished with an iris diaphragm which can be adjusted to match the effective numerical aperture of the objective Leica DM750 M 1 To open and close this diaphragm simply turn the knurled ring on the condenser to the rightorto the left so that the line on the ring is aligned with the objective magnifi cation used The line on the ring is aligned with the objective magnification used Manual 2 Open the iris diaphragm of the condenser completely by turning the condenser ring all the way to the right Preparation for Viewing a Specimen Slide 1 Position a specimen slide on the specimen 2 Position the specimen slide such that a Stage by sliding it under the slide grips part of the specimen is under the objective used H Slide grips hold the slide in place Slide grips Leica DM750 M Manual Focusing 1 Rotate the objective nosepiece in such 2 Move the specimen stage upwards by a way that the objective with the lowest turning the coarse focusing knob as far as magnification level is in the working posi it will go to the maximum position tion Leica DM750 M Manual 3 Look into the eyepieces and adjust the illu mination intensity to the desired level The stand of the Leica DM750 M has been H calibrated at the factory so that the focus can be found from this position within 1 5 rota tions of
14. lling Objectives When rotating the objective nosepi A ece always use the knurled ring on the objective nosepiece While rotating the objective nosepiece clock wise screw the objectives into the objective nosepiece Start with the lowest magnification Leica DM750 M Manual Conversion to Incident Light Polarization Installing the polarizing adapter 3 Insert the polarizer into the polarizing 5 Push the analyzer into the opening as far as 1 Unscrew the screw and remove the cover adapter it will go The opening for the polarizing adapter is now accessible 4 Remove the cover of the analyzer 2 Insert the polarizing adapter into the opening and tighten the screw Leica DM750 M Manual 15 Use Leica DM750 M Manual 16 Switching on the Microscope Precautionary measures The light of the Leica DM750 M can be A very bright Therefore only look into the eyepieces after you have switched on and reduced the illumination see item 3 Connecting and switching on the micro scope 1 Connect the power supply unit and the power cable with the incident light axis of the Leica DM750 M and secure the plug using the retaining ring on the incident light axis Leica DM750 M Switch on the illumination by briefly pres H The brightness can be adjusted in 15 sing the power switch increments Look through the eyepieces and adjust the brightness to the desired level Manual 17 Modifyi
15. m 44 Leica DM750 M Manual 2 Safety instructions Safety Notes A Before installing operating or using the instrument it is mandatory to read this user manual In particular please observe all safety instructions The Safety Concept brochure contains additi onal safety information regarding service work requirements and the handling of the micro scope the electrical and other accessories as well as general safety instructions You can combine individual system articles with articles from external suppliers Please read the user manual and the safety instructions of the supplier To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals Leica DM750 M Warning of a danger This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to follow these inst ructions may pose hazards to personnel cause instrument malfunctions or damage the instru ment Hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to follow these inst ructions may pose hazards to personnel cause instrument malfunctions or damage the instru ment Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Manual EC Declaration of Conformity El
16. ng the Aperture Diaphragm Aperture diaphragm Opening closing the aperture diaphragm The aperture diaphragm of the incident 1 Close the aperture diaphragm by moving light axis can be opened or closed An the lever upwards open aperture diaphragm generates a higher resolution with a decreased depth of field A 2 Open the aperture diaphragm by moving closed aperture diaphragm on the other hand the lever downwards reduces the quantity of light and resolution capacity but the depth of field is increased A The aperture diaphragm must be completely open for all oblique light illu mination scenarios Leica DM750 M Manual Illumination Scenarios Illumination Scenarios You can adjust the incident light axis Li illumination to your needs and require ments using the membrane control panel A The aperture diaphragm must be completely open for all oblique light illu mination scenarios Leica DM750 M Changing the illumination mode Press the button in the middle once to switch between bright field illumination and oblique illumination oblique incident illumination Bright field illumination E O Oblique illumination Manual Individual light field oblique light Hold a button pressed longer than two seconds to activate only one of the four light fields for oblique illumination Combinations with other light fields are also possible OOO O O 4 RJE O O Individual l
17. pply at any time The power cable is provided as the power disconnect device Instructions on use continued Intervention from the user The microscopes of the DM series contain H no components that can be maintained or repaired by the user Exceptions are menti oned specifically in this manual Unless otherwise specified in this manual repair and service work may be carried out by authorized Leica technicians only Unauthorized alterations to the instru ment or noncompliant use shall void all rights to any warranty claims Leica DM750 M Maintenance Leica DM series microscopes are basi H cally maintenance free To ensure that it always operates safely and reliably we recom mend that you take the precaution of contac ting the responsible service organization Here you can arrange for periodic inspections and conclude a maintenance contract recommen ded Accessories Only those accessories may be used that are listed in this user manual or for which safe use has been confirmed by Leica Microsystems Manual Danger of infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups Assembling the Leica DM750 M Assembling the Incident Light Axis Assembly 3 Fasten the incident light axis into place by 1 Place the incident light a
18. the fine focus 4 Bring the specimen into focus using the fine focusing knob 32 Viewing Tube Adjustment Adjust the eyetubes 1 Adjust the tubes to your interpupillary distance Fold or unfold the eyetubes to decrease or increase the distance between the eyepieces until you see one illuminated circle Leica DM750 M Leica EZ Viewing Tube If you are using a Leica EZ Viewing Tube which has eyepieces that are integrated into the tubes no further adjustments are required Simply wear your glasses or contact lenses For Leica DM750 M with Koehler field diaphragm continue with the Koehler Configuration section on page 36 Manual Standard Viewing Tube with Two Fixed Eyepieces If you are using a standard tube with two fixed eyepieces no focusing eyepieces no further adjustments are required Simply wear your glasses or contact lenses For Leica DM750 M with Koehler field diaphragm continue with the Koehler Configuration section on page 36 33 Viewing Tube Adjustment cont Standard tube If you are using a standard viewing tube with one or two focusing eyepieces you need to make some adjustments 1 Set the focusing eyepieces to 0 If you are comfortable wearing your H corrective lenses contact lenses or eyeglasses for microscope viewing leave them on and your adjustments will be minimal Leica DM750 M 2 Bring the specimen into focus using the fine focus setting kno
19. xis with the heat tightening the setscrew sink facing backwards onto the tripod mount 2 Align the incident light axis according to the tripod geometry Leica DM750 M Manual 8 Assembling the Viewing Tubes There are two types of viewing tube Assembly 2 Set the dovetail into the tripod mount and Identify which viewing tube you have 1 Loosen the setscrew on top of the stand tighten the setscrew carefully This way using the Allen key provided the tube automatically shifts to the correct position on the optical axis of the micro scope Type 1 Leica EZ Viewing Tube with integrated eyepieces Type 2 Standard tube with separate eyepieces Leica DM750 M Manual 9 Leica EZ Viewing Tube with Integrated Eyepieces 1 In order to use the wingscrew remove the setscrew that was delivered with the stand Leica EZ Viewing Tube with integrated eyepieces Loosen the setscrew at the stand or H replace the setscrew with the optional wingscrew to rotate the Leica EZ Viewing Tube 2 Detach the wing screw part completely before the screw is used on the stand 3 Reinstall the Leica EZ Viewing Tube onto the stand 4 Tighten the wingscrew using the wrench included in the delivery package Leica DM750 M Manual Leica EZ Viewing Tube integrated eyepieces cont Now you can rotate the Leica EZ Viewing Tube by loosening the wingscrew brin ging the tube to the desired position and tigh
Download Pdf Manuals
Related Search
Leica_DM750M_Manual_EN