Hardware User`s Manual Shocker without scrambler
Contents
1. 0 dw _ CLEANING EA CLEANING Smuw 0 M STERLIZING Sm M STERILIZING mmu M STERILIZING See chapter 9 5 warning Shocker without scrambler Harvard Apparatus 16 Panlab Acids 12 TECHNICAL SPECIFICATIONS POWER SUPPLY Input voltage Frequency Fuse Maximum Power Conducted Noise 115 230 VAC 50 60 Hz 2 fuses 5mm 20mm 100mA fast 20W EN55022 CISPR22 CISPR16 class B ENVIRONMENTAL CONDITIONS Operating temperature Operating relative humidity Storage temperature 10 to 40 C 0 to 85 RH non condensing o C to 50 C non condensing SHOCKER Range Selector omA to 1 9mA DC Steps of o 1mA DC LICK DETECTION Maximum impedance detection Lick detection current lt 0 55uA DIMENTIONS control unit Width x Height x Depth Weight 161mm x 67 mm x 188 mm 8509 Shocker without scrambler 17 Panlab jade ir DECLARACI N DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMIT Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornella de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto VOGEL SHOCKER Declares under his responsibility that the product D clare sous sa responsabilit que le produit2. 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Shocker without scrambler Panlab flicker ke 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original pac
3. Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt 1 lt Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o a
4. Marca Brand Marque PANLAB Modelo Model Modele LE 100 25 Cumple los requisitos esenciales establecidos por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mecanica Machinery directive Directive m canique Para su evaluacion se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class B FCC FCC47CFR 15B Class B Safety of machinery EN ISO 1
5. The interconnection between the cages and the computer is carried out through an RS 232 serial communication Such a configuration allows the test to be performed on a desktop Output is optically isolated from ground WARNING If you touch at the same time the bottle tetine and the grid while an experiment is being conducted you may receive electrical shock Shocker without scrambler Panlab jade ir 7 EQUIPMENT DESCRIPTION 7 1 LE100 25 FRONT PANEL POWER SHOCK TEST LICKS SHOCKER INTENSITY E 100 25 Vogel Shocker Power Intensity Shock Test Bye EIN mA Figure 7 LE100 25 Front Panel e POWER 3mm red led that remains ON while control unit is on e SHOCK TEST Shock can be tested by pressing this button In this case shock will be activated while the button is pressed e LICKS 3mm green led that comes on each time a lick is detected e SHOCK 3mm red led that comes on whenever the shocker is active manually by pressing the SHOCK TEST button or remotely activated through the software e INTENSITY Decimal selector used to select shock intensity from o 13mA DC to 1 9mA DC in steps of o 1mA DC Shocker without scrambler Panlab 7 2 LE100 25 REAR PANEL LINK BOX CAGE POWER Link Box Figure 8 LE100 25 Rear Panel e LINK BOX RJa1 telephonic female connector used to connect the LE100 25 with the Link Box o1 Each Link Box is able to control up to 8 LE100 25 s The
6. aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales a otras formas de reciclaje de a usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente 5 Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique D
7. 2100 2010 En consecuencia este producto puede incorporar el marcado CE y FCC Consequently this product can incorporate the CE marking and FCC C En consequence ce produit peut incorporer le marquage CE et FCC En representacion del fabricante Manufacturer s representative En representation du fabricant Carme Canal s 77e General Manager No Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 25 06 2014 Shocker without scrambler 18 Harvard Apparatus Panlab H Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment taf Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se
8. G THE BOTTLE STERILIZE THE CAGE THE FRID AND THE BOTTLE TROUBLESHOOTING PREVENTIVE MAINTENANCE TECHNICAL SPECIFICATIONS Shocker without scrambler Harvard Apparatus 10 11 13 13 13 13 13 14 15 16 17 Panlab H avala 6 INTRODUCTION The Vogel test is a conflict test that has become a standard for fast screening of the potential anxiolytic properties of drugs In this procedure drinking behaviour is punished by mild electrical shocks leading to a significant reduction of water consumption in depressed animals Drinking responses are then re established by drugs with anxiolytic properties Figure 6 LE100 25 Vogel Shocker Control Unit The Panlab Vogel test consists of a standard home cage associated with a grid floor An electronic unit associated with a special nipple ensures the detection and counting of the licks reflecting the animal s drinking behaviour An exclusive nipple design ensures that any casual or non specific contacts of the animal with the nipple will not be considered as a drinking response The multi cage configuration of the Vogel test associated with the Packwin software allows performance of the Vogel test for up to 32 cages The cages are associated with a link box 1 for every 8 cages ensuring the functional interaction between the lick sensor system the shock generator 1 per cage and the PackWin software for advanced protocol configuration and data acquisition
9. Hardware User s Manual Shocker without scrambler Vogel Test References LE100 25 76 0334 Version Vo3 11 2014 Panlab H Harvard Apparatus Panlab s l u C Energ a 112 08940 Cornella de Ll Barcelona Spain www panlab com International Calls 34 934 750 697 Domestic Call 934 190 709 Fax 34 934 750 699 Info panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab Ades ur 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be N exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C AN Warning about a metal surface that can supply electrical shock when it s A touched Decontamination of equipments prior to disposal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEEE A
10. e when necessary OPENING FLANGE SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT OR VOLTAGE SETTING CHANGE In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment or if the equipment voltage setting is incorrect check fuses according to the following procedure 1 Remove power cord from the power inlet Shocker without scrambler Harvard Apparatus Panlab H 2 Open fuse holder by pulling the flange with a regular screwdriver Figure 2 Open fuse holder door 3 Extract fuse holder using the screwdriver CORRECT INCORRECT Figure 4 Fuses position 5 Insert the fuse holder again positioning it according to the voltage in the AC net 115V POSITON 230V POSITION Figure 5 Fuse holder position 6 Ifthe fuses blow again unplug the equipment and contact technical service N For electrical safety reasons never open the equipment The power waRNING SUpply has dangerous voltage levels Shocker without scrambler Panlab 5 TABLE OF CONTENTS 9 3 9 4 9 5 10 11 12 SYMBOLS TABLE GOOD LABORATORY PRACTICE UNPACKING AND EQUIPMENT INSTALATION MAINTENANCE TABLE OF CONTENTS INTRODUCTION EQUIPMENT DESCRIPTION LE100 25 FRONT PANEL LE100 25 REAR PANEL EQUIPMENT CONNECTION WORKING WITH THE UNIT CONDUCTING AN EXPERIMENT CLEANING THE CAGE CLEANING THE GRID CLEANIN
11. ected to the Computer Com Port e Remote Port of each Link Box is connected to the Main Port of the next Link Box e Remote Port of the last Link Box is left free e Allthe ID numbers must be different Shocker without scrambler 12 Panlab 9 WORKING WITH THE UNIT 9 1 CONDUCTING AN EXPERIMENT To conduct an experiment with the unit proceed as follows L 7 Connect all the cables as explained in Chapter 8 of this manual Place the animals in the experimentation cages Turn on all the units Within the PackWin software load an existing Protocol or edit a new one See Vogel Test working procedure for further information Run the protocol to proceed with the experiment Once the experiment has ended remove the animals from the experimentation cages Clean the experimentation cages to prepare for a new experiment 8 Turn off all control units 9 2 CLEANING THE CAGE To clean the cage you can use a slightly wet cloth and then dry them with a dry cloth If it is too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth 9 3 CLEANING THE GRID To clean the grid you can use a slightly wet cloth and then dry them with a dry cloth If it is too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth 9 4 CLEANING THE BOTTLE To clean t
12. esigned be controlled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Shocker without scrambler Panlab H avala 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e The user is only authorised to replace fuses with the specified typ
13. he bottle you can use a slightly wet cloth and then dry them with a dry cloth If it is too dirty you can wet the cloth with a soapy solution to clean them then remove foam with a wet cloth and finally dry them with a dry cloth Shocker without scrambler 13 Harvard Pa n la b 9 5 STERILIZE THE CAGE THE FRID AND THE BOTTLE Either cage or grid are autoclavable Before sterilizing them you should disconnect the tweezers that are not ready to resist the heat autoclavable Plastic parts of the tetine must not be autoclavable otherwise q WARNING Only the body of bottle and metal parts of the tetine are they will be damaged Shocker without scrambler 14 Panlab Ades ur 10 TROUBLESHOOTING This table features instructions to solve the most frequent problems PROBLEM SOLUTION Ensure that the voltage of mains is the same as that selected in the The equipment does not start up fuse holder Check the condition of the fuses Verify that all cables are connected see Chapter 8 Check that both control units LE100 25 Vogel Shocker and Link Box o1 are powered Verify that the current decimal The animal does not receive shock selector is not set to o omA Check the protocol you are using in Packwin Verify that the selected serial port in Packwin match the computer serial port Shocker without scrambler 15 Panlab 11 PREVENTIVE MAINTENANCE EXPERIMENT MONTHLY
14. king has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the Original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits N For electrical safety reasons you only can connect equipment to WARNING power outlets provided with earth connections E This equipment can be used in installations with category ll over voltage according to the General Safety Rules The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Shocker without scrambler Panlab Control Some of these instruments d
15. ltre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente Nota em Protecc o Ambiental Ap s a implementac o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este s mbolo no produto o manual de instruc es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuic o para a protecc o do ambiente 5 Shocker without scrambler 19
16. maximum number of cages to be connected to one computer is 32 This is done by using 4 Link Box 01 s connected in series e CAGE A stereo jack is used to connect the LE100 25 with the grid and the nipple It supplies the shock to the animal whenever it drinks e POWER Power inlet main switch and fuse holder Shocker without scrambler 10 Panlab notara 8 EQUIPMENT CONNECTION The following illustration features an example of the connection of 2 Vogel Test Cages with one Link Box 01 and one computer LINK BOX 01 On TS La S8 amp T 86 Figure 9 Example of connection of 2 cages The necessary cables and connections are listed in the following table FROM CABLE LE100 25 Cage Nipple and Grid Stereo jack to 2 bananas LE100 25 Link Box Link Box Port r RJ11 telephonic cable Link Box o1 Main Computer Com Port RS 232 cable This connection is repeated for each LE100 25 This connection is repeated for each LE100 25 3 N is the number of port among 1 and 8 Shocker without scrambler 11 Panlab B 90 One computer is able to control up to 32 LE100 25 s In this case it will be necessary to connect 4 Link Box 01 s to the computer in series 8 LE100 25 s will be connected to each Link Box 01 Figure 10 Series connection of the Link Boxes The following rules must be met e Main port of the first Link Box is conn
Download Pdf Manuals
Related Search