UVD 170S/340S UV/VIS Detector Manual
Contents
1. Order No Description Quantity Fuse 1A 5 x 20mm 2 available as spare part under order no 6065 9002 Kit Fuses UVD see section k 2 1272 0003 l 1310 1501 l 1310 7031 or Power cable 220V 3 x 0 75mm 2m 1 1310 7032 Power cable 115V 3x AWGI18 2m depending on country 7146 1051 21462625 l 2261 0121 2 2261 0122 2 2309 1100 l 6007 9100 l 8005 9001A Connector cable remote input pressure output 1 The order number always refers to the packing unit For further information please contact your Dionex sales representative 8 2 Spare Parts Order No Description 1343 0511 Quartz lens UVD170S 340S standard flow cell 2101 0303 M3 knurled flow cell retaining screw 2200 5502 Single part hand tight fitting 2251 6002 PEEK capillary tube 1 16 x0 50mm Im 2261 0121 PEEK fitting screw 1 16 15 mm 2261 0122 PEEK double ferrule 5053 1204 D lamp 5065 1800P Flow cell UVD170 340 6 ul preparative complete 5065 1800M Micro glass capillary flow cell complete 5065 1810 Flow cell UVD170 340 standard biocomp 10u1 complete 5065 1811 Lens retaining platel 5065 1812 Lens retaining plate2 24 Order No 5065 1815 5065 1820 6065 1800 6065 9001 6065 9002 9365 0001B Operating Instructions UVD 1708 3408 Description Heat exchanger flow cell analyt confg including Heat exchanger PEEK fitting screw 1 16 15 mm Capillary tube 1 58x0 25 ID Single part hand t2. Lamp is still too hot CHROMELEON message Lamp too hot Lamp cannot be switched on CHROMELEON message Lamp On failed Connecting cable between UVD 170S 340S and PC not correctly installed Detector not connected to mains Detector not switched on Fuse blown Replaced fuse blows immediately Connecting cable between UVD 170S 340S and PC not correctly installed Flow cell contaminated Low transmission through mobile phase Incorrect spectral range Faulty injection valve Fault in diode array Lamp intensity too low M68 board defective Connecting cable between UVD 170S 340S and PC not correctly installed Remedial action Allow a delay of 5 minutes then switch the lamp on again Allow a delay of 5 minutes then switch the lamp on again If several attempts are unsuccessful check the lamp and the connecting cable see below Connectors on the detector and the PC must be inserted evenly and locked into place with latching devices on both sides Connect mains cable Switch on detector Replace fuse Call Dionex service Connectors on the detector and the PC must be inserted evenly and locked into place with latching devices on both sides Clean flow cell gt section 6 3 4 Select alternative wavelength Check solvent for contaminants Alter spectral range Check fluidics Call Dionex Service Change lamp Check M68 board replace Connectors on the detector
3. fig 6 The input capillary is marked by the black color Connect this capillary to the column output Li Please note In order to minimize peak broadening effects due to dead volume it should be ensured that all fluidic connections between sample entry and detector are of minimum possible length A Important The max pressure rating of the flow depends on the model gt Technical page 23 For the standard flow cell the pressure rating is 100 bar 10 000kPa A Important The flow cell is primed with 2 propanol 2 4 Rear Panel Connections The UVD 1708 3408 is controlled under the CHROMELEON data system via a specific PC interface board Data transfer is via a special cable order no 1310 1501 which is connected at the rear of the unit A Important Use only the original UVD 170S 340S connector cable and extension order no 1310 1502 from Dionex The Remote Input provides a digital interface that can be used in CHROMELEON e g to synchronize the Inject signal of an Autosampler or manual injection valve with the analysis start of the data system RER eee PC CONNECTOR VERBINDUNG PC wO wO z2wO VOLTAGE SELECTOR g WITH MEIN USE REMOTE INPUT es 1207 batt SPANNUNGSWAHLER MIT HAUPTSICHERUNG o an a POWER SUPPLY O DT NETZANSCHLUSS POWER SWITCH Eoin HAUPTSCHALTER NZ ru34 2 dwg NZ Fig 3 Rear
4. not attempt to remove the flow cell body from its housing Clean the flow cell body together with the housing Preparative Flow Cell Order no 5065 1800P The preparative flow cell for the UVD 170S 340S has a volume of 6ul and a path length of 2mm The cell body is composed of PEEK and is thus 100 biocompatible The maximum pressure rating of the flow cell is 100bar 10 000kPa A Important When cleaning the flow cell gt section a4 please note that only the lens retaining plates and the lenses may be removed Do not attempt to remove the flow cell body from its housing Clean the flow cell body together with the housing Micro Glass Capillary Flow Cell Order no 5065 1800M The micro flow cell has a volume of 0 14 ul at a path length of 8 mm The flow cell body is 100 biocompatible The maximum pressure rating of the micro flow cell is 400bar 40 000kPa A Important Please note that it is not possible to disassemble the micro flow cell for cleaning Clean the micro flow cell by flushing with 0 2M nitric acid followed by water and methanol 20 Operating Instructions UVD 170S 340S 6 3 2 Flow Cell Removal e If the UVD 1708 3408 is still connected to the data system first terminate communication using the Disconnect command in CHROMELEON gt CHROMELEON manual Switch off the detector Disconnect the power cord Open and remove the side panel Do not bend the Teflon capillary Undo the 4 retaining screws a
5. panel UVD 1708 3408 Operating Instructions UVD 1708 3408 2 5 Installation on the PC Interface Board amp i Htr D a U nH I U En as lo GS PAL Ta D Fi TJ O k t EE 5 Fig 4 Address selection on the M68 Interface board For selection of the I O address the board is fitted with a DIP switch block Dual Inline Package as shown in fig 4 The board occupies 4 addresses starting at the selected base value which is factory set at 318H Under normal circumstances this value need not be changed unless operating in combination with network and or other interface boards the addresses of which should be confirmed before installation Warning Ensure that the PC is off and the power cable disconnected before attempting installation A Important The interface board must be protected against static charge at all times If difficulty is encountered fitting the board into the PC please contact the Dionex Service Li Please note The board can be inserted in an 8 bit or 16 bit slot on 5 IT on S yy al hte on a ee An open switch OFF indicates a binary 1 while a closed
6. resulting from inappropriate use service or the implementation of non specified spare parts Similarly the warranty coverage shall be invalidated in the event of inappropriate shipment packaging or failure to remove aggressive or damaging solvent residues All user serviceable parts i e lamp and flow cell are accessible via a removable panel located on the left side of the unit fig 6 To open press in and turn the knurled screw 90 anti clockwise The panel may now be opened and removed The lamp and flow cell are now clearly visible Warning During operation the lamp becomes extremely hot and remains so for some time after the unit is switched off Therefore to avoid possible injury always allow sufficient time for lamp cooling before attempting any maintenance 15 Operating Instructions UVD 170S 340S Deuterium lamp Deuteriumlampe eGo Flow cell cover Me zellenverschlu KL Flow cell cover removed Me zellenverschlu ge ffnet Flow_cell Me zelle o Knurled retaining screw Markin R ndelmutter Markierung 6 Inlet EinlaB Outlet Ausla LD TI TI ay seitansi Fig 6 Side view UVD 170S 340S side panel open 16 Operating Instructions UVD 170S 340S 6 2 Lamp A Important Use only the original Dionex deuterium lamp Using third party parts may damage the instrument and t
7. sides Shorten or replace for narrower capillaries Rinse or replace column Replace with fresh solvent Use fresh sample or alter conditions Change gradient program check pump Ensure constant temperature and humidity Rinse or replace column CHROMELEON Error Messages A list of CHROMELEON error messages is included in th OMELEON Installation program on the tab dialog box Error Levels See also section 14 Operating Instructions UVD 170S 340S 6 Maintenance 6 1 General Information The UVD 1708 3408 is constructed only from the highest quality components thus keeping maintenance requirements to a minimum In general the unit should be kept clean The painted surfaces are resistant to solvents as well as weak acid or alkaline solutions If solvents or other liquids should be spilled on the surface these should be cleaned off immediately using a lint free cloth or tissue avoid rubbing The following sections describe all maintenance work which can be carried out by the user Any other service or maintenance work should be carried out only by a qualified Dionex personnel In the event that the UVD 170S 340S should require shipment for the purpose of service this should be only in the original packing If no original packing is available this can be acquired from Dionex or your local Dionex representative Li Please note The warranty coverage shall become invalid in any case identified as
8. Configuration Close the CHROMELEON Installation Tool 10 Operating Instructions UVD 170S 340S 4 3 4 Operation within CHROMELEON In CHROMELEON there are two principal modes of instrument operation e direct control via the toolbar and menu bar or via controls in the Control Panel e control via time programs PGM file or command buttons The following signal specific and global commands are available Signal specific commands Signal type Parameters UV channel Step Average Wavelength Bandwidth Ref Wavelength Ref Bandwidth Signal type Parameters 3D Field only UVD Min Max Wavelength 340S Bunch Width Step Ref Wavelength Ref Bandwidth Global commands Lamp on off Autozero Connect Disconnect For detailed information on controlling the detector via CHROMELEON refer to the CHROMELEON User Manual and context sensitive online Help in CHROMELEON 11 Operating Instructions UVD 170S 340S 4 3 5 Spectra Calibration under CHROMELEON Under CHROMELEON spectra calibration is performed automatically after each Lamp on or Connect command detector calibration The command CheckWavelength allows you to include the largest wavelength deviation of this calibration in the Audit Trail A calibration is possible only when certain conditions are met e During calibration the baseline must be sufficiently stable This
9. EZ DIONEX UV VIS Detector UVD 170S 340S Operating Instructions ia Version 3 01 Date July 14 2000 Now sold under the Thermo Scientific brand Dionex Softron 2000 Il hermo Doc UVD170SUVD340_OI_E_V301 doc SCIENTIFIC Ey DIONEX CE Declaration of Conformity Product UV VIS Detector Type UVD 170S 340S Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations e EN 50081 1 1992 Electromagnetic Compatibility EMC Generic emissions standard Part 1 Residential commercial and light industry e EN 50082 1 1992 Electromagnetic Compatibility EMC Generic immunity standard Part 1 Residential commercial and light industry e EN 61000 3 2 1998 Electromagnetic Compatibility EMC Part 3 Section 2 Limits for harmonic current emissions This declaration is issued for the manufacturer Dionex Softron GmbH Dornierstrasse 4 D 82110 Germering by the President Dr Peter Jochum January 16 2001 Operating Instructions UVD 170S 340S Contents Operating Instructions UVD 170S 340S 1 Introduction 1 1 Unpacking To unpack the unit proceed as follows e Place the box on the floor and remove the accessories pack e Pull out the unit slowly and carefully using the two foam inserts e Remove the polythene packaging by placing the unit on a firm base and lifting at one side then the other while
10. and the PC must be inserted evenly and locked into place with latching devices on both sides 13 Problem Strong baseline drift High noise level Peak broadening Analysis is not reproducible Insufficient warm up time Column contaminated System not equilibrated Unstable environment Solvent contaminated Flow cell contaminated Lamp defective Solvent reservoir is placed too low Solvent contaminated Lamp aged Gas bubbles in system Pressure pulsation from pump Solvent reservoir is placed on the floor Wrong wavelength Connecting cable between UVD 170S 340S and PC not correctly installed Capillaries too long or has excessive internal diameter Column overloaded contaminated or aged Solvent aging Sample is unstable Irreproducible gradient Unstable environment Column overloaded contaminated or aged Operating Instructions UVD 170S 340S Probable cause Remedial action Allow min 30 min warm up Rinse or replace column Continue to rinse system until equilibrated Ensure constant temperature and humidity Change solvent Clean flow cell gt sec Replace lamp Place reservoir on the same level as flow cell Replace solvent Replace lamp Prime system Check and prime pump Place reservoir on the same level as flow cell Select suitable wavelength Connectors on the detector and the PC must be inserted evenly and locked into place with latching devices on both
11. creased noise 17 Operating Instructions UVD 170S 340S 6 3 Flow Cell A Important The flow cells of the detectors UVD 170S 340S and UVD 160S 320S are not interchangeable A Important The resistance of the flow cell depends on the solvents This applies particularly to solvents which can form active radicals or peroxides 6 3 1 Flow Cell Versions The following flow cells are available for the UVD 1708 3408 detector e standard flow cell order no 5065 1810 e standard flow cell with PEEK capillaries order no 5065 1820 e preparative flow cell order no 5065 1800P e micro glass capillary flow cell order no 5065 1800M Standard Flow Cell Order no 5065 1810 The standard flow cell of the UVD 170S 340S has a volume of 10ul and a path length of 9mm The flow cell body is composed of PEEK with quartz windows and is thus 100 biocompatible The inlet capillary is factory fitted with a single part handtight fitting The outlet is a PEEK capillary Optionally a PEEK capillary can be used for the inlet The maximum pressure rating of the standard flow cell is 100bar 10 000kPa A Important When cleaning the flow cell gt section Sa please note that only the lens retaining plates and the lenses may be removed Do not attempt to remove the flow cell body from its housing Clean the flow cell body together with the housing 18 Fig 7 Standard Flow Cell Operating Instructions UVD 170S 340S uvd_messzel
12. drawing the packaging out from underneath it i Please note Retain all original packing materials in a safe place It is the optimum packaging for shipping the unit e g for repair Shipping of the unit in any other packaging automatically nullifies the warranty e Check off the contents of the accessory pack against the list in section Standard Accessories UVD 170S 340S page 1 2 Warranty The standard warranty coverage for this unit is in accordance with the conditions of sale The warranty has a duration of one year from invoice date and covers materials and labour ex works Please note that wear parts i e needle needle seat syringe and valve seals cannot be covered by the warranty The warranty period for the deutertum lamp is six months from delivery date see the label at the lamp cable Removing this label automatically invalidates the warranty coverage For lamps with timer without the above label the warranty period is 1000 operating hours The warranty does not extend to the flow cell The warranty coverage shall become invalid in any case identified as resulting from inappropriate use service or the implementation of non specified spare parts Similarly the warranty coverage shall be invalidated in the event of inappropriate shipment packaging or failure to remove aggressive or damaging solvent residues 1 3 How to Use This Manual The layout of this manual is designed to provide quick reference to the app
13. h or tissue e Clean the lenses using a soft lint free cloth or tissue and an optical cleaning solution e Scratched or damaged lenses must be replaced A Important Do not attempt to remove the flow cell body 8 from its housing 10 e Cleaning of the flow cell body together with its housing may be carried out by rinsing with water iso propanol or methanol or by placing the entire assembly into an ultrasonic bath 21 Operating Instructions UVD 170S 340S A Important Never attempt to remove dirt from the flow cell body using a spatula tweezers or any other objects as this may lead to irreparable damage e Replace the lenses onto either side of the flow cell body so that the flat faces are on the inside e Replace the retaining plates 6 and 9 taking care to tighten the screws 5 evenly and without excessive force e Check the flow cell for leakage before replacement into the unit tightening screws 5 if necessary 22 Operating Instructions UVD 170S 340S 7 Technical Specifications Optics Light source Wavelength range Pixel bandwidth Wavelength accuracy Noise Drift Flow cell volume Flow cell pressure rating Measuring range Time constant PC connection Data channels UVD 3408S Data channels UVD 170S Wetted parts Power requirements Power consumption Dimensions Weight Operating conditions Single beam principle with simultaneous measurement across
14. he warranty coverage shall become invalid 6 2 1 Lamp Removal e If the UVD 170S 340S is still connected to CHROMELEON first terminate communication using the Disconnect command in CHROMELEON gt CHROMELEON manual e Switch off power to detector and remove mains connection e Open and remove the side panel see above Do not bend the teflon capillary e Wait until lamp has cooled down e Disconnect the lamp by gently pressing either side of the locking plug and pulling out e Undo and remove the two lamp retaining screws using a 2 5mm Allen key and carefully remove the lamp The Allen key is contained in the Accessories Warning During operation the lamp becomes extremely hot and remains so for some time after the unit is switched off Therefore to avoid possible injury always allow sufficient time for lamp cooling before attempting any maintenance 6 2 2 Lamp Replacement e Insert the new lamp aligning the notch on its base with the alignment pin of the mounting and replace the retaining screws No additional alignment is required A Important Avoid touching the glass tube of the lamp Reconnect cable Replace the side panel e The detector may now be switched on and communication with CHROMELEON restored using the Connect command Li Please note A new lamp must be run in for at least 24 hours prior to the first analysis During this period there may be strong baseline fluctuations and in
15. ight fitting Screw M3x12 DIN965 2 pcs Standard flow cell UVD170 340 10 ul PEEK capillaries complete Repair kit flow cell analyt including Screw M3x23 DIN 965 3 pcs Quartz lens 2 pcs Lens retaining platel 1 pc Lens retaining plate2 1 pc UVD 340S 170S Repair Kit Quartz lenses 2 pcs PEEK capillary tubing 1 16 0 50 mm 2 m PEEK fitting screws 1 16 15 mm 2 pcs PEEK double ferrule 2 pcs deuterium lamp D 2 pcs Kit Fuses UVD including Fuse 0 80A medium slow 5x20 mm 1pc Overload fuse 1A slow 5x20 mm 2 pcs Overload fuse 2A slow 5x20 mm 2 pcs M68 PC Interface board The order number always refers to the packing unit For further information please contact your Dionex sales representative 25
16. imum length to avoid peak broadening due to excessive dead volume A Important Do not place any objects or stack additional instruments on top of the detector The ventilation slits on top of the unit must remain free at all times 2 2 Mains Connection All units are generally factory set for operation at 230V In the event that the mains supply varies from this it is necessary to alter the setting of the voltage selector located on the rear of the instrument immediately above the mains socket gt figs 1 and 3 The alteration procedure is as follows e Switch off the unit and disconnect the power cable e Draw out the fuse cartridge using a small screwdriver gt fig 1 e Using small pliers tweezers to pull out the small voltage selection board from the power supply module e Place the board writing facing up under the required voltage selection as shown in fig 2 e Turn the board so that the voltage selection can be seen as shown in fig 2 e Adjust the plastic clip as shown in fig 2 without turning the board Ensure that the clip locates in the notch on the board e Now replace the voltage selection board all the way back into the power supply module gt fig 2 e Finally replace the fuse cartridge A Important Upon installation of the instrument for the first time the earth connection and fuses should be checked A Please note For minimum interference effects all components of the analysis system sho
17. le dwg No Description Order No 1 Screw M3x12 DIN965 included in 5065 1815 2 Heat exchanger included in 5065 1815 3 PEEK tubing ID 0 5mm 2251 6002 4 PEEK fitting screw 1 16 15 mm included in 5065 1815 5 Screw M3x23 DIN 965 included in 6065 1800 6 Lens retaining platel 5065 1811 7 Quartz lens 1343 0511 9 Lens retaining plate2 5065 1812 11 Single part hand tight fitting included in 5065 1815 12 Capillary tube 1 58x0 25 ID included in 5065 1815 13 PEEK double ferrule 2261 0122 Order No Description 5065 1815 Heat exchanger flow cell analyt confg including Heat exchanger PEEK fitting screw 1 16 15 mm Capillary tube 1 58x0 25 ID Single part hand tight fitting Screw M3x12 DIN965 2 pcs 6065 1800 Repair kit flow cell analyt including Screw M3x23 DIN 965 3 pcs Quartz lens 2 pcs Lens retaining platel 1 pc Lens retaining plate 2 1 pc 19 Operating Instructions UVD 170S 340S Standard Flow Cell with PEEK Capillaries Order no 5065 1820 The standard flow cell with PEEK capillaries has a volume of 10ul and a path length of 9mm Inlet and outlet are PEEK capillaries The flow cell is 100 biocompatible The maximum pressure rating of the PEEK flow cell is 100bar 10 000kPa A Important When cleaning the flow cell gt section kaa please note that only the lens retaining plates and the lenses may be removed Do
18. may not be the case e g when the solvent composition was modified or when there were air bubbles in the solvent e The solvent in the cell must not be fully absorbing in the wavelength range which shall be calibrated This will be the case e g if the cell is filled with 96 hexane 4 ethyl acetate e Ensure for the calibration that the deuterium lamp is already warm because its spectrum changes much during the first minutes after switching on the lamp If these conditions are not met the process will be interrupted with the corresponding error message When the problem is solved repeat the calibration using the command Disconnect first followed by Connect To calibrate the detector the transmission spectrum of the deuterium lamp is compared with that of the holmium oxide filter With the resulting spectrum the maxima are determined and compared with the holmium oxide values stated in the literature If for a maximum a difference is detected between the measured and the known value an interpolation is performed between this maximum and the two adjacent maxima to correct the wavelength allocation of the affected photodiodes The spectra calibration can take up to 2 minutes During this time data acquisition is not possible 12 5 Troubleshooting Operating Instructions UVD 170S 340S Problem Lamp is not switched on No function No signal irregular signal No signal irregular signal Probable cause
19. nd remove the flow cell cover Undo the knurled flow cell retaining screw and carefully draw out the flow cell assembly Warning During operation the lamp becomes extremely hot and remains so for some time after the unit is switched off Therefore to avoid possible injury always allow sufficient time for lamp cooling before attempting any maintenance 6 3 3 Flow Cell Replacement A Important The flow cells of the detectors UVD 170S 340S and UVD 160S 320S are not interchangeable e Insert the new cleaned flow cell aligning the notch with the alignment pin of the mounting No further alignment is necessary Replace the flow cell cover e Replace the side panel e The detector may now be switched on and communication with CHROMELEON restored using the Connect command 6 3 4 Flow Cell Cleaning A Important In contrast to the other flow cell versions it is not possible to disassemble the micro flow cell for cleaning Clean the micro flow cell by flushing with 0 2M nitric acid followed by water and methanol To clean the flow cell except the micro flow cell proceed as described below The numbers in parentheses refer to the corresponding parts as illustrated in fig 7 e Undo and remove screws 1 and remove the heat exchanger 2 e Undo and remove screws 5 and carefully remove the lens retaining plates 6 and 9 and lenses 7 e To avoid scratching the lenses these should be placed on a clean lint free clot
20. ng Gitter 5 152 schema34 Fig 5 Principle of operation UVD 1708 3405 Operating Instructions UVD 170S 340S 4 Operation The UVD 170S 340S detector is intended only for operation with the CHROMELEON data system Stand alone operation is not possible Data transfer is performed digitally via a special interface cable section b 4 4 1 Switching the Unit On Off Having ensured all cable connections to the UVD 1708 3408 the unit can be switched on via the power switch on the rear of the unit Li Please note Li Please note A Important The deuterium lamp is not automatically switched on Lamp on off is performed by CHROMELEON For optimum results the lamp should be switched on at least 30 minutes prior to analysis After the Lamp off command allow 5 minutes before switching the deuterium lamp on again Otherwise the lamp becomes too hot and could be damaged message Lamp too hot In the event that the PC is switched off or the connection cable to the UVD 170S 340S is disconnected the deuterium lamp is automatically switched off Prior to connecting or disconnecting the UVD 170S 340S cable to the PC the unit must be switched off Ensure that the connectors on the UVD 170S 340S and on the PC are inserted evenly and that they are correctly locked into place via the latching devices on both sides 4 2 Status LED on the Front Panel When the detector is s
21. ropriate sections according to the operation required However it is recommended that before operating the UVD 1708 3408 the manual should be read thoroughly and completely in order to obtain a full understanding of the instrument Operating Instructions UVD 170S 340S At various points throughout the manual messages of particular importance are indicated by the following symbols whose relevance is as follows A Please note Indicates general information to assist optimum performance to be obtained A Important Indicates that failure to take note of the accompanying information may result in damage to the instrument Warning Indicates that failure to take note of the accompanying information may result in personal injury 1 4 Intended Use of This Instrument The UVD 1708 3408 is a high sensitivity UV VIS diode array detector and is specifically intended for use only for HPLC analysis and only under the control of the CHROMELEON Data System Dionex shall not be liable for any damages material or otherwise which are caused by inappropriate or improper use of this device Operating Instructions UVD 170S 340S 2 Installation 2 1 Positioning and Environment The location of the instrument should conform to the following e stable and free of vibration e free of large temperature variation and draughts e away from direct sunlight capillary connections between the column and the flow cell should be of min
22. ssary change settings Tab Dialog Box General Option Description Instrument name under which the detector is listed in the installation environment default UV Port Address V O Base address of the PC interface board default 318H Demo Mode Deactivate the demo mode Device Name Operating Instructions UVD 170S 340S Tab Dialog Box Signals Option Description UV VIS 1 UVD 170S UVD 340S UV VIS 2 UVD 170S UVD 340S UV VIS 3 UVD 170S from CHROMELEON v 4 10 UVD 340S UV VIS 4 UVD 1708S from CHROMELEON v 4 10 UVD 3408 3DFIELD only UVD 3408 A Important Ensure that the check boxes of the required channels are activated If not these channels will not be available in CHROMELEON Select the required signal and press Change to view the following signal parameters Signal parameter Description Sal Nand Signal unit of the recorded raw data Factor current amplification factor Tab Dialog Box Error Levels In this box you can view the classification of errors in certain error levels The default settings must not be altered Tab Dialog Box Inputs Option Description INPUT170 only UVD 170S INPUT340 only UVD 340S A Important Ensure that the check boxes corresponding to the remote inputs are activated If not these inputs will not be available in CHROMELEON Finally save the modified Server
23. switch ON indicates a binary 0 On the right the values are converted into hexadecimal values Determine the address by adding the values The default setting of the UVD 170S 340S interface board therefore has the following base address 8 10 100 200 318H hexadecimal Operating Instructions UVD 170S 340S 3 Overview The UVD 170S 340S is a high performance diode array detector for HPLC detection in the UV VIS wavelength range The unit has been developed exclusively for operation under the CHROMELEON chromatography data system The UVD 170S 340S features Powerful optics combined with extremely low noise electronics enable highest sensitivity Simultaneous detection on 4 wavelengths recording of 3D data fields and the comprehensive data analysis capabilities within the CHROMELEON data system provide maximum analysis efficiency Li Please note The model UVD 170S supports simultaneous detection on 4 wavelengths if operation is via CHROMELEON from version 4 10 Recording 3D fields is not possible with this model 3 1 Principle of Operation Deuterium lamp Deuterium Lampe Column Trenns ule Flow cell Me zelle Addresses Waste Adressen 7 Data System Abflu Il 7 FH l l l ESS E gt s 25 A D a Array Multiplexer Amplifier Converter Interface PC Verst rker Wandler Schnittstelle Grati
24. the photodiode array Deuterium lamp 200nm 344nm UV range 345nm 595nm VIS range 1 9nm UV range 3 3nm VIS range 0 75nm UV range 1 5nm VIS range lt 0 8 x 10 AU after 3h warm up at 254nm time step 1sec empty flow cell lt S5X 10 AU h after 3h warm up at 254nm empty flow cell Standard flow cell 10ul 9mm path Standard flow cell PEEK capillaries 10u1 9mm path Preparative flow cell 6ul 2mm path Micro capillary flow cell 0 14ul 8mm path Standard flow cell 100bar 10 000kPa Standard flow cell PEEK cap 100bar 10 000kPa Preparative flow cell 100bar 10 000kPa Micro capillary flow cell 400bar 40 000kPa OAU to 2AU 0 1sec Proprietary cable 4 3D field with or CHROMELEON control option 4 with CHROMELEON as of version 4 10 PEEK quartz 115 230V 10 50 60Hz SOVA 210 x 480 x 430mm w x h x d 20kg Temperature 10 C to 35 C Air humidity 40 to 85 Technical information October 1998 subject to alteration without notice 23 Operating Instructions UVD 170S 340S 8 Accessories and Spare Parts Spare parts and accessories are always maintained at the latest technical standard Therefore order numbers are subject to alteration However updated parts will always be compatible with the parts they replace 8 1 Standard Accessories UVD 170S 340S The following standard accessories order no 5065 9000 are included in the shipment
25. uld be connected to the same mains output D Warning Before attempting to alter the voltage selection or replace fuses always ensure that the power cable is disconnected 2 2 1 Fuse Replacement Operating Instructions UVD 170S 340S Switch off the unit and disconnect the power cable draw out the fuse cartridge using a small screwdriver gt fig 1 replace fuses as appropriate A Important Only use fuses as indicated in fig 1 min voltage 250 V or those listed in the original parts list US type fuse US Gr e If using US type fuses follow the procedure shown in fig 1 Replace the fuse cartridge Fuse Feinsicherung 5x20mm 250V FUSES USE ONLY WITH DIN 41662 2 pin fuse protection 2 polige Absicherung Insert direction Arrows aligned Einsteckposition Pfeil auf Pfeil Loosen screw and turn board Schraube l sen und Aufnahmeplatte wenden NO 120 2404 Insert in this direction Einschubrichtung spann3 dwg Fig 2 Voltage selection 6 3x32mm UL 198 G 1 pin fuse protection 1 polige Absicherung sichfas dwg Fig 1 Removing the fuse cartridge from the power supply module Operating Instructions UVD 170S 340S 2 3 Fluidic Connections The capillary connections of the UVD 170S 340S are located on the left side of the detector gt
26. witched on the status LED on the front panel indicates the current status of the deuterium lamp LED LED red LED green LED off Status D2 Lamp Instrument is switched on Deuterium lamp is switched on Instrument is switched off Operating Instructions UVD 170S 340S 4 3 Installation and Operation within CHROMELEON 4 3 1 Communication Port The UVD 1708 3408 is controlled via a special PC interface board section p a 4 3 2 Required CHROMELEON Modules To be able to control the detector UVD 170S 340S via CHROMELEON the corresponding CHROMELEON Control Option is required For questions please contact your Dionex sales representative 4 3 3 Installation with the CHROMELEON Installation Tool Proceed as follows to install the detector UVD170S 340S in the CHROMELEON Installation Tool e Start the CHROMELEON Server and then the CHROMELEON Installation Tool Server Configuration e Select the timebase to which you want to assign the detector UVD170S 340S e Via the Edit menu or the menu of the right mouse button choose Add Device e Choose Dionex UVD170S or Dionex UVD340S from the displayed list Confirm by pressing OK The now displayed tab dialog boxes show the current configuration of your detector Generally these settings must not be changed However please check whether the settings correspond to your current installation environment If nece
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