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Adam-SCC USER`S MANUAL

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1. 23 4 Press the P Run button on the main device Automatic Focus will be carried out the first time the device is booted Shut down the device properly and the following time the device is turned on the Auto Focus process will be skipped gt HE 12 00 gt 12 00 TE aes N 345 T23 N 345 5 After acquiring the image the chip will be ejected automatically Then chip can be removed 6 The calculated cell number per 1ml will be displayed and printed automatically f 12 00 E 12 00 x1024 mL x1024 mL 1024 T Check sample 512 N Over range If the density of sample is over range you may see Check Sample or Over Range message at display monitor If you see those messages please check below list first lt In case of Check sample gt l Check sample contamination or Chip with dust or other materials 2 Check mixture of sample and reagent It has to be mixed well 3 Check test tip whether stained with something If you have problems that mentioned above you will get a result sheet like below 2009 11 03 12 12 03 2009 11 03 12 12 03 2009 11 03 14 38 12 2009 11 03 14 38 12 2010 08 06 10 18 09 2010 08 06 10 18 09 2006 11 06 15 33 38 2006 11 06 15 33 38 2006 11 06 15 37 42 2 A2 2 sa 24 lt In case of Over Range gt 1 In case of the result of cell counting is over 4000 X1024mL 2 Check mixture of sample and agent It has to be mixed well If you hav
2. In order to check whether the device is working properly turn on the instrument and press Nm Lock The LED lamp should be flickering If the lamp is not flickering or the keypad does not work properly this might be a result of a connection error In this case turn off the power check the connection and reboot The available keys are number keys Nm Lock Enter and only And please ignore others 000650000 Fan lt Power switch Power plug Key pad port RS 232C port BNC port USB port Figure 4 The rear side of the main device Power switch Main power ON OFF control Power plug Connects the Adam power cord to wall outlet Fan Adam s cooling fan Keypad port Key pad connector RS 232C port Serial cable connector USB port USB serial port BNC port CCD camera cable connector 2 3 Paper Roll Refill Method To insert or replace the paper roll of thermal printer see figure shown below Ensure that the paper roll is installed in right direction Figure 5 The method of paper roll refill CAUTION In order to print the results using the thermal printer check that the printer cover closed and that the paper roll installed properly If the printer cover is open or there is not enough paper roll the print function would not work Paper roll will rotate and come out once the FEED button is pressed 2 4 Menu setting You can set the menu as you press
3. OSB Transfer Sizes Cancel Select lower settings to correct performance problems at low baud rates Select higher settings for faster performance Receive Bytes 4096 hal Transmit Bytes 4096 ag EM Options Defaults Hee E Miscellaneous Options Select lower settings to correct response problems senal Enumerator z Serial Frinter Cancel lf Power OFf Latency Timer meec 16 Timeouts Set ATS On Close Minimum Read Timeout msec lo Disable Modem Ctrl At Startup Minimum Write Timeout msec fo r r Event On Surprise Removal F r r 7 Click the OK button 14 3 3 USB Serial Driver Installation 1 To install the Adam USB serial driver follow the directions below First insert the installation CD ROM into the computer Then open the file CDM_ Setup exe 2 Click Ok to finish the installation FTDI Driver Installation x a LA FTOI COM Drivers have been successfully installed 3 4 USB to serial driver installation If experiencing trouble using the serial port COM1 use the USB to Serial device If comport in your PC is not working or does not have a serial port This is an example in how to use the pl 2303 model If you use another USB to Serial Device you should install suitable device driver file To find the device driver file visit the company s web site to find additional support Install pl 2303 model device dri
4. Ports COM amp LPT If the USB serial port 1s not set to COMS change it to COMS 4 Click the right mouse button on USB Serial Port COM6 and click on Properties 12 E Device Manager EA CRP H Y Computer Hs Disk drives fa E Display adapters aS Floppy disk controllers 0 3 Human Interface Devices IDE ATAJATAPI controllers Intel AIM 3 0 Codec EH Keyboards Matrox Imaging Adapter E Mice and other pointing devices E Monitors Network adapters El 4 Ports COM amp LPT 28 Communications Port COM1 e vee A Communications Pork COME vee A Communications Pork COMS vee A Communications Pork COM oo Printer Port LPT1 po USB Serial Pork COME H Processors 0 8 sound video and game H System devices E Universal Serial Bus co 6 Change the COM Port Number from COM6 to COM 5 13 COM Port Number COME USB Transfer Sizes Select lower settings to dorrect performance problems at low baud rates Select higher settings for faster performance Receive Bytes Transmit Bytes BM Options Miscellaneous Options Serial Enumerator a Serial Printer Cancel lf Power OFf Latency Timer meec Timeouts Minimum Read Timeout imsec lo Minimum Write Timeout msec fo Set ATS On Close r r Event On Surprise Removal F r Disable Modern Ctrl At Startup T Advanced Settings for COMIG COM Port Number COM
5. ADAM is not working Check the connection cable and power Turn on ADAM and check working Check the COMPORT settings in Section and Section 2 At low temperatures lt 10 C please warm up the system for 10 minutes before use 066006 If you experience any further problems contact us 2 USB to Serial Port is not COMS in my PC D Check COMPORT as detailed in Section 1 and Section 2 2 If you experience any further problems contact us 3 My PC does not have a serial port D Use USB to Serial Device as explained in Section 2 2 If you experience any further problems contact us 4 Please let us know if you see an error message or the following step does not operate properly ADAM SCC System State Digital Bio coin Error 0x00030000 28 7 Technical Specifications e Hardware Objective lens 4 x Laser 532 nm CCD 1 3 inch B W CCD Display 128 x 64 LCD Monitor Printer Thermal printer 57 5 W mm paper roll Stage controller e Dimensions 220 L x 350 W x 250 H mm e Weight Approximately 10 kg e Power AC 115 230 V 6 3A 50 60 Hz e Environment Condition 5 lt T lt 40C rH lt 80 Altitude lt 2000 m e Loading Volume Prior to analysis the sample is mixed with a stain solution 20 ul is recommended for each channel in Two Channel Chip e Measuring Time About 1 min test e Measurement Range Operational 5 x 10 4 x 10 cells ml 29 e Accessor
6. SCC Kit Test kit Reagent amp Two or four channel chip SCC Kit i 3 3 l C Pi Y A i Adam SCC Main device SCC Kit HF Power cord Tf Keypad Fuse AS x 5 E n d j ar Paper roll USB cable RS 232C serial cable Installation CD Figurel The components of the Adam SCC The excitation source is a green laser The emission filter removes all wavelengths except red fluorescent light The red fluorescent light from the particles in the sample is focused onto the detector CCD camera Then the image analysis program counts the red particles to represent the cell number in the sample The Adam s SCC Kit 1s intended for easy use in farming or milk truck 100 ul of stain solutions are prepared separately in small tubes CRS K01 100 test pack or CRS K02 400 test pack One kit is capable of 100 tests Each tube has 100 ul reagent of somatic stain solution The disposable chip 1s a significant plastic disposable for loading and detecting of the sample The chip loads the volume of 20 ul for SCC kit CRS KO1 2 channel chip and 12 ul for SCC kit CRS K02 4 channel chip and with the height of 100 um The chip is treated by gas plasma to get a hydrophilic surface of channels which drives the sample flow through the channel 2 Hardware Section 2 1 Front side of Adam LCD 2 Door LED lamp Printer LED
7. 10 3 1 Installation Of SOPTWAME cccc ccc ccccccceccccccceccccccccecececcaccececceauuecsceuauaunenenenenass 10 3 2 Port setting With AdAmM oooocccccconconccccconccnnccnnonnnnonoonancncononcnnnnnncnnonnnrnnononens 12 3 3 Installation of USB serial driver ocoococoocococcococconocconnncnnnnononanonnnnnnons 15 3 4 USB to serial driver installation 0ocoococcococncconccconoconnnnnncnnnonnnnnnnns 15 3 5 Communication port Check oooccccccccccccooonccnnnccncnnononcnnnnnnnnononanenononenonnnns 17 3 6 Description of the control software occccccccoccconccccooccnncocnancnononnnanonnnnos 18 4 Measurements Section ccccccccccecccceccececcececcececcecucccuceceucucuecseauceuaeaeceueceuaeees 20 4 1 Materials 8 Metho0d 000o0ocococococococococononononononononononononononononnnnnnnnnonons 21 0 A 1 eh ete eet ee ne ne ee ee eee 22 O Tro DleSROOUNG secs cscs as analisis io ate 28 7 Technical specificationsS cccoonccococonconccoocnncononcncononcnnnnnnnnnonnnncnnnnancnnnnncnnnnnos 29 1 System Components When opening the Adam SCC you will find the following components in your package Instrument parts Adam SCC main device 1 unit Power cord 1 5 m 1 ea Paper roll 57 5 mm 2 roll Installation CD 1 ea USB cable 1 ea RS 232C serial cable 1 ea Keypad ea Consumable parts
8. Es Communications Port COn LY USB Serial Port CONS E Sound video and game controllers H Storage volumes a3 System devices l Universal Serial Bus controllers 3 6 Description of the Control Software Report program Interface to manage and report all results from the main device All measurement results are automatically saved on the memory of the main device excluding experimental images The user can also download and delete the individual data from the memory and export it to Excel xls format AN CAUTION Before running the program check the connection of two cables RS 232C amp USB between the Adam and the PC or desktop Then check the communication port on the PC or desktop and refer to Section 3 5 a Report Program DBT DER s o o A O ka www digital bio corn The Art of Micro In Vitro Diagnosis ee 2009 11 03 2009 11 03 2009 11 03 2009 11 03 2010 08 06 2010 08 06 2006 1 1 06 2006 11 06 2006 11 06 2006 11 06 2006 11 06 2006 11 06 2006 11 06 2006 11 06 2006 11 06 2006 11 06 2006 11 07 2006 11 07 Count SCC 1000 mly 12112 05 12 12 05 14 50 12 14 50 12 10 18 09 10 18 09 15 3335 15 33 35 last te 15 3742 16 10 44 16 10 44 17 0729 170729 172057 17 20 57 10 48 50 10 45 50 T M T N T M T M T M T K T M T M T Fi Figure 6 The main report program Type Ti A Total Count TrA Read Data Delete Export Eo Excel 18 1 Por
9. be installed to following folder Click lt Install gt to begin installation to following folder To install to a different folder Either enter install path Or click lt Browse gt and select another folder space needed 1 KBytes 3 Click Ok to finish the installation A Installation of Repor Operation Software y1 0 0 0 Installation of Report Operation software 41 0 00 completed 4 Ifthe installation was successful the report program can be found at Start gt All Program gt Report Operation Software gt Report 3 2 Port setting with Adam The fist time using the ADAM monitor or report program you must confirm the comport settings In the desktop window follow the steps below 1 Start gt Control Panel gt System 2 Click on Device Manager System Properties x System Restore Automatic Updates Remote General Computer Hame Hardware Advanced Device Manager The Device Manager lists all the hardware devices installed on your computer Use the Device Manager to change the properties of any device Driver Signing lets you make sure that installed drivers are compatible with Windows Windows Update lets you set up how Windows connects to Windows Update for drivers Driver Signing windows least Hardware Profiles Hardware profiles provide a way for you to set up and store different hardware configurations Hardware Profiles coca 10 3 Click on
10. lamp amp Control buttons Control buttons Key pad Figure 2 The front side of the main device LCD Displays the process and the results Door Chip holder comes out here Ow O Printer Print the experiment information using the thermal printer An example of a printout is shown below 4 LED lamp amp Control buttons e Parking Protect the alignment of stage from external shock when the main device is moved to the other places e Start Run Performs all procedures of automatic counting or changing the mode for inputting cow numbers e Eject Ejects the chip holder from the Adam MIR MEH ME ADAM SCC Model name WME ADAM SCC HF 2007 11 20 15 57 33 Prints date amp ti e ET 2007 11 20 15 57 33 Farm s name a A E O E A li e Cow s name T 1130 SCC X1000 m Ea T channel MAD T1 1115 SCC X1000 me Count result H 1142 SCC X1000 me N channel lt 1413 1115 SCC X1000 me Oy is Result of 2 channel chip CRS KO1 TZ 1149 SCC X1000 me MPG N2 1065 SCC X1000 me EE Ei e cm e E DE ES Cr FS pr o CS E ee ES FS SE E E Result of 4 channel chip CRS K02 Figure 3 The example of the result Used after inputting the sample numbers or setting the menu is complete CAUTION The keypad should be connected to the device before the power is turned on Otherwise the instrument will not work properly
11. NESMU ASC 001E V 0 Microchip type Automatic Cell Counter ADAM Adam SCC USER S MANUAL A Digital Bio Adam series User s Manual All the materials in this manual are protected by Korean and international copyright laws This manual may not be reproduced translated published or distributed by any means without the permission of the copyright owner ADAM Adam SCC User s Manual Homepage www digital bio com E mail Sales digital bio com bus Developed and Manufactured by NanoEnTek Inc 12F Ace High end Tower 235 2 Guro 3dong Guro gu Seoul 152 711 Korea Tel 82 2 6220 7911 Fax 82 2 6220 7721 E Mail sales digital bio com The information in this manual is described as correctly as possible but 1t may be changed without prior consent or notification Copyright 2007 by NanoEnTek Inc All rights reserved Published in Korea Documentation NESMU ASC 001E V 0 Oct 10 2008 10 17 Contents 1 System Components ocooooocccccccccccnonocccnnnnononnnnnnnnnnnnononnnnnnnnnnnnonnnnnncnnnnnnnnonnnnnennnnnnnnnnos 1 2 Hardware Section oooccoccccococcocnococcnnoncnnnnno nono nn non nn rr nn nn nn nnnnnnnns 3 2 1 Front side Of Adam ccccccccceccccccecccececcececucccuccucecscueauaesecscauaeuecscauauaenerseanaenes 3 2 4 Menu SCI O siria nr itdr 8 3 Software Section 2 2 0 0 cc cc ccccccccccceccccecccceccccucccecccecuccuceeuucecuecucusucueaesscueaecsuaeceuaeceeaeees
12. e problems that mentioned above you will get a result sheet like below 12 12 03 2009 11 03 12 12 03 2009 11 03 14 38 12 2009 11 03 14 38 12 2010 08 06 10 18 09 2010 08 06 10 18 09 2006 11 06 15 33 38 2006 11 06 15 33 38 2006 11 06 15 37 42 SH FAFA Fazal 7 For another experiment repeat the process from steps 4 7 5 4 Channel Chip CRC T02 Cell Counting 1 Screen for inputting the sample numbers will appear and it requires the inputting of three digits Press Enter key once the digits have been inputted ry HE 12 00 gt gt Input cow number MANDA T2 N2 2 Press A EJECT button on the main device to eject the chip holder 3 Insert the Chip loaded with the sample onto the chip holder Please be careful not to make bubbles Sample Preparation Please refer to the 4 1 Material amp method section for the detailed operation a Insert Direction 4 Press the P Run button on the main device Automatic Focus will be carried out the first time the device is booted Shut down the device 25 properly and the following time the device is turned on the Auto Focus process will be skipped p ES E gt ES E T1 45 Tie 45 T2 N2 T2 N2 5 After acquiring the image the chip will be ejected automatically Then chip can be removed 6 The calculated cell number per 1ml will be displayed and printed automatically HEEE HEEE x1024 mL x1024 mL 1024 Check samp
13. fter pressing the Enter key the screen will return to the Menu screen automatically System Info 1 Version SCC V1 0c 2 Date Nov 19 07 CORE1 0 20 CORE2 0 20 CORES 0 20 Press the key after menu setting Once inputted the screen will return to the counting mode automatically 3 Software Section 3 1 Software Installation To install the Adam SCC Report Operation software follow the directions below First insert the installation CD ROM into the computer Then open the file Setup _Report_v1 0 exe The start up dialogue of the software will appear It is recommended that the Digital Bio folder gt Report is located in the default directory of the local hard disk When the location has been selected click Install to proceed with the installation The computer activates the Installation of the Software 1 Click Next to continue A Installation oft Repor Operation Software 1 0 0 0 Saks This will install Report Operation software 1 000 on your computer lt is recommended that you exit all other programs before proceed with installation Click lt Next gt to continue installation Or click Cancel to cancel installation Cancel 10 2 Click Install to begin installation to C Program Files Digital Bio Report folder 2 Installation of Report Operation Software 1 0 0 0 Seles Report Operation Software 41 0 0 0 will
14. ies Power cord 1 5 m Fuse 250 V AC 3A Paper roll 57 5 W mm e Related products CRS KO1 SCC Kit 50 pcs Soma Chip CRC T01 100 ul Stain solution 100 ea Max 100 tests kit CRS K02 SCC Kit 100 pcs Soma Chip CRC T02 100 ul Stain solution 400 ea Max 400 tests kit 30 ye A Digital Bio Contact Information NanoEnTek Inc Tel 82 2 6220 7911 Fax 82 2 6220 7721 E Mail sales digital bio com
15. le 512 45 512 1024 eo 1024 512 Over range If the density of sample is over range you may see Check Sample or Over Range message at display monitor If you see those messages please check below list first lt In case of Check sample gt 1 Check sample contamination or Chip with dust or other materials 2 Check mixture of sample and agent It has to be mixed well 3 Check test tip whether stained with something If you have problems that mentioned above you will get a result sheet like below Number Date Time Cow Number _ _Count scc 1000 ml 2000 2009 11 03 12 12 03 2009 11 03 12 12 03 2009 11 03 14 38 12 2009 11 03 14 38 12 2010 08 06 10 18 09 2010 08 06 10 18 09 2006 11 06 15 33 38 2006 11 06 15 33 38 2006 11 06 15 37 42 22 2 2 2 510 lt In case of Over Range gt 26 1 In case of the result of cell counting is over 4000 X1024mL 2 Check mixture of sample and agent It has to be mixed well If you have problems that mentioned above you will get a result sheet like below 2009 11 03 12 12 03 2009 11 03 12 12 03 2009 11 03 14 38 12 2009 11 03 14 38 12 2010 08 06 10 18 09 2010 08 06 10 18 09 2006 11 06 15 33 38 2006 11 06 15 33 38 2006 11 06 15 37 42 N T N T N T N T 7 For another experiment repeat the process from steps 4 7 caution At low temperature lt 10 C please warming up the system for 10 min 6 Trouble shootings 1
16. nto the T N ADAM SCC such as eject run parking or insert Status of the Chip Holder 22 Displays the status for Cell Counting After you press the run button Displays the status of the Chip Holder is being ejected After you press the eject button Displays the status of Chip Holder is being parked After you press the park button Displays the status of Chip Holder is being inserted Displays the status when you use the 2 Channel Chip CRC TO01 refer to the 2 Chip Selection of Menu setting on the page 13 Displays the status when you use the 4 Channel Chip refer to the 2 Chip Selection of Menu O 0800 u setting on the page 13 4 2 Channel Chip CRC T01 Cell Counting 1 If no system error is found Adam is ready to receive the sample numbers Screen for inputting the sample numbers will appear and it requires the inputting of three digits Press Enter key once the digits have been inputted AN CAUTION Inserting numbers is not possible with a PC or desktop keyboard Please use the provided keypad only c E 12 00 gt gt Input cow number 1 123 N 345 2 Press A EJECT button on the main device to eject the chip holder 3 Insert the Chip loaded with the sample onto the chip holder Please be careful not to make bubbles Sample Preparation Please refer to the 4 1 Material amp method section for the detailed operation j Insert Direction
17. ounting using the Adam A raw milk sample SCC Kit Pipette and tips 1 The prepared materials ready to be used Raw Milk ee oo SCC Kit lt o 2 Add 100 ul of the raw milk sample in tube DN 4 e 3 Mix the sample by turning the vial upside down 3 5 times S i 4 4 Load 20 ul for SCC kit CRS K01 2 channel chip or 12 ul for SCC kit CRS K02 4 channel chip of the cell sample onto the Chip Ensure that no bubbles enter the channel Insert the chip into the chip holder and push the run button 21 ae Operation of the Adam 1 Check the connection of the main device power cord 2 Make sure that the main power switch is in the 1 ON position On the rear side of the main device 3 Check initial LCD display of the READY state cy 12 00 ADAM SCC l Digital Bio gt gt Input cow number p T N Booting 7 The screen above is the first display of the ADAM when you turn on the ADAM MC It starts to run system check automatically And the next screen follows continually 1f there 1s no system error ADAM SCC System State S Digital Bio Booting Error 0x00030000 Please let us know if the first screen does not run to the next display automatically or the error message comes on the screen Status of setting menu ADA li 5 12 00 gt gt Input cow number ds gt Displays the order status of the performances o
18. ransfers the report to Excel format and saves it Saved location Default C Report Excel File name Default Report Date Time xls A Measurement Section SCC Kit CRS K01 is composed of Propidium iodide PI for counting somatic cells CRS KO1 can be used without diluting raw milk Measuring range of cell density is 0 to 4 x 10 cells ml Appropriate range of the sample mixture is 5x 10 to 1 x 10 cells ml One kit is capable of 100 tests Each tube has 100 ul reagent of somatic stain solution Simply add the same volume of the raw milk sample in the tube then every preparing for experiment end Once the experiment is complete the results can be printed through the thermal print Printed number indicates cell concentration x 1000 ml in each channel T The cell concentration in the T channel of the chip N The cell concentration in the N channel of the chip Store kit box upright and at room temperature Expiration date of stain solution is written on the bottom of the kit box yy mm dd Be sure to check the expiration date before using Follow the exact steps detailed in the Instructions for Use section 1 Add 100 ul of the raw milk sample in the tube 2 Mix the sample thoroughly by turning the vial upside down 3 5 times 3 Load 20 ul of the cell sample onto the Chip Ensure that no bubbles enter the channel 20 4 1 Material amp Method The following list contains the items needed for somatic cell c
19. t Setting and Application Selection Using the right button on the mouse click Port Setting first and confirm as bellows Number Date Time _ Cow Number Count SCc 1000 ml _ 200000 2009 11 03 12 12 03 00000 00005 T E 200001 2009 11 03 125 01 00003 Soo00z 2009 1103 14 lop 00286 pul Peace Viability Port Setting To set the communication port Com port COM 1 USB port COM 5 Pont Setting Com Pork 1 com 1 Usb to Serial port coms SCC To be used by the database of the main device 2 Experiment list The experiment list window consists of the number date time cow number counting result and channel type 2009 11 03 12 12 03 5 00001 2009 11 03 12 12 03 00001 N 500002 2009 11 03 14 38 12 00002 00286 T 5 00003 2009 11 03 14 38 12 00003 00165 N 5 00004 2010 08 06 10 18 09 00004 00510 T 5 00005 2010 05 06 10 18 09 00005 00683 N 500006 2006 11 06 15 33 38 00006 00896 T 500007 2006 11 06 15 33 38 00007 00801 N 200008 2006 11 06 15 37 42 00008 se T After sample test if the sample result is Range over 1t describes result as R O and the result is Check Sample it describes result as S E 19 3 Function Buttons Total Count 4 Shows the number of experiment data AS Loads the experiment data from the memory of the main device Delete Hild Deletes all of the loaded data and memory of the main device Export to Excel T
20. the button on the keypad from the screen for inputting cow numbers 2 12 00 gt gt Input cow number Configuration e 1 LCD contrast 2 Chip selection 3 System info Tes N You can find the setting modes by selecting the number from the Menu 1 LCD contrast Press the number 2 key to adjust the brightness of the LCD screen from the MENU Configuration e LCD Contrast 1 LCD contrast 2 Chip selection 3 System info Value 660 Range 600 750 LCD Contrast Value 660 Value is rerecorded Range 600 750 Adjust LCD contrast if the letters do not appear clearly on the screen Press the Enter key after you input the three digits The range is from 600 to 750 After pressing the Enter key the screen will return to the Menu screen automatically 2 Chip Selection User can select the 2 kinds of chip type One is the two channel chip for SCC Kit CRS KO1 Another is the four channel chip for SCC Kit CRS K02 Configuration e Chip Selection 1 LCD contrast 1 2 channel chip 2 Chip selection 3 System info 2 4 channel chip Choice 1 Press the Enter key after you input the three digits After pressing the Enter key the screen will return to the Menu screen automatically 3 System Information The device versions and date which have been installed in the device can appear when the number 4 key is selected from the MENU A
21. ver PS d_pl2s03h hx _v20019v202 1 1 Double click on PL 2303 Driver Installer exe 2 Click Next to continue 15 InstallShield Wizard Welcome to the InstallShield Wizard for PL 2303 USB to Serial The InstallShield Wizard wall install PL 303 LISB to Serial on your computer To continue click Next 3 Click Finish to complete the installation InstallShield Wizard InstallShield Wizard Complete InstallShield already successfully to installed PL 2303 USB to Sernal diver Please press Finish button to close and out of InstallShield Fou have been plugged cable on PC before run this setup please unplug and then plug cable again for system detect this device Black Cancel 16 3 5 Communication Port Check 1 Check the connection between the main device and computer via the USB to serial line 2 Check the communication port and USB serial port START gt Control Panel gt System gt Hardware gt Device manager gt Port Com amp LPT s Fie Action View Help gt m faea E Computer Hss Disk drives E Display adapters Ae Floppy disk controllers H A IDE ATAJATAPI controllers 38 Intel AIM 3 0 Codec Matrox Imaging 4dapter H t Mice and other pointing devices FH Monitors H E Network adapters E 6 Ports COM amp LPT Bo bes Communications Pork COM1 4 von E Communications Port CORA e E Communications Port COM enn

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