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Frac950 User Manual

1. To computer UniNet 1 Figure 2 15 Connecting Frac 950 UniNet 1 to an AKTAexplorer system 3 Connect the UniNet 1 cable running to the computer to one of the free UniNet 1 connectors in Frac 950 Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 25 Installation 2 9 Connecting to a supply voltage 26 mains socket WARNING The unit must be connected to a grounded To locate the Mains connector in Frac 950 see Figure 2 13 Any mains voltage of 100 120 220 240 V AC 50 60 Hz can be used Connect the supplied mains cable between Frac 950 and a mains socket at the rear of the AKTAdesign chromatography system The example in Figure 2 16 shows connection to an AKTAexplorer system Frac 950 To mains socket To Frac 950 Figure 2 16 Connecting Frac 950 mains supply to an AKTAexplorer system Note Frac 950 contains no user replaceable fuse Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Installation 2 10 Inserting collection tubes Fraction Collector Frac 950 WARNING Be sure to fold down the safety bar whenever the rack holder is operated by hand This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced Insert sufficient collection tubes into the rack gently pushing each one down as far as it wil
2. M gt Retaining screw t 2 Dispenser arm shaft Figure 2 26 Removing the dispenser arm shaft 1 Unscrew and remove the retaining screw 1 2 Remove the dispenser arm shaft 2 Fraction Collector Frac 950 38 User Manual 18 1139 56 Edition AE Rack holder Fraction Collector Frac 950 Installation Installing standard mode components Install the standard mode components as shown in Figure 2 27 Delivery arm lid Rack holder lid Rack holder retaining screw Figure 2 27 Installing standard mode components 1 Fitthe rack holder using the keying guide to position it correctly 1 Insert and tighten the rack holder retaining screw 2 Insert the rack holder lid 3 Remove the delivery arm lid 4 Loosen the Phillips screw 5 o O A Q N Place the delivery arm in standard mode position 6 Lift approx 0 5 cm and turn the delivery arm forward until it snaps firmly in position 7 Tighten the Phillips screw and refit the lid For installing standard mode racks see Assembling standard mode racks on page 16 User Manual 18 1139 56 Edition AE 39 Operation 3 Operation 3 1 On off A Pinch Hazard 40 WARNING Pinch hazard Do not have any part of your body within the unit base area when Frac 950 is switched on An automatic calibration process starts when Frac 950 is connected to UNICORN During calibration the dispenser arm
3. Figure 2 13 Frac 950 rear panel with connectors DropSync connector To locate the DropSync connector see Figure 2 13 Run the cable from the DropSync unit in the cable recess in the delivery arm and down the rear of the circular stand as shown in Figure 2 14 and connect it to the DropSync connector Both cables are run in the cable recess DropSync cable Accumulator cable Figure 2 14 Cable runs for the DropSync unit and accumulator cables Accumulator connector To locate the UniNet 3 connector see Figure 2 13 Run the cable from the Accumulator unit in the cable recess in the delivery arm as shown in Figure 2 14 and connect it to one of the UniNet 3 connectors Fraction Collector Frac 950 24 User Manual 18 1139 56 Edition AE Installation 2 8 Connecting to a UniNet 1 communication link Frac 950 is controlled from a PC running UNICORN version 3 2 or higher using UniNet 1 cables Prep Mode requires version 4 0 CAUTION The mains power to the AKTAdesign chromatography system must be switched OFF before connecting Frac 950 to the UniNet 1 link Disconnect the UniNet 1 cable running from your AKTAdesign system to the computer Run a new UniNet 1 cable included from the AKTAdesign system to one of the UniNet 1 connectors in Frac 950 The example in Figure 2 15 shows connection to an AKTAexplorer system e ENS Frac 950 e E
4. as follows 1 2 N A AW Bs In UNICORN start the pump manually In System Control select Manual Frac Select Man_Fractionation for the new mode See also section 3 2 Operating Frac 950 Stop the run immediately when the first tube shift has been reported by clicking on the END button in the toolbar Disconnect the system in UNICORN Switch off Frac 950 at the mains switch on the rear panel Switch on Frac 950 Connect the system in UNICORN When the system has connected to the Frac 950 again it should be positioned at the waste position of the new mode Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Installation Removing prep mode components Remove the dispenser arm and the rack as shown in Figure 2 25 Finger tight D connector spenser arm 3 Knurled screw 1 5 A 7 gt Waste arm l NSS z Figure 2 25 Removing the dispenser arm and the rack 1 Fold down the safety bar Loosen the finger tight connector and pull out the capillary 1 Loosen the knurled screw 2 Remove the dispenser arm 3 Push the waste arm to the right 4 N Ua A Q N Lift up and remove the rack 5 Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 37 Installation 7 Remove the dispenser arm shaft as shown in Figure 2 26
5. changed to an optional configuration Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 41 Operation 3 3 Collecting fixed fractions Details about collecting fixed fraction volumes using Frac 950 in a method are described in the AKTAdesign Optional Configuration User Manual 3 4 Collecting peak fractions Details about collecting peaks only using Frac 950 in a method are described in the AKTAdesign Optional Configuration User Manual 3 5 Feed tube During fractionation the tube rack can be moved forward one tube with the instruction FeedTube 1 Select menu System Control Manual Frac in UNICORN 2 Select the instruction FeedTube in the Frac list WINCORN LAB14NT Frac Instructions x r Instructions p Parameters l EA C Pump Man Fractionation 1 FractionationSto Mie Man_Peak_Fracti E an Peak Fractionation Alams Mon Peak FracStop Frac Peak_FracParameters Accumulatorw ash Close Other Ignore LastTube L Help 3 Click on the Execute button The tube rack moves on to the next tube after the set delay volume has been collected Fraction Collector Frac 950 42 User Manual 18 1139 56 Edition AE 3 6 Operation Ignore LastTube The tube position in the rack which is defined in the start protocol to have the last tube can be ignored with the instruction Ignore LastTube When the last tube is reached and there are mo
6. direct sunlight The unit may be operated at temperatures in the range 4 to 40 C The unit should be installed on a stable laboratory bench The recommended position for Frac 950 is immediately to the right of the AKTAdesign chromatography system as shown in the example below Figure 2 1 Frac 950 placed together with an AKTAdesign system Fraction Collector Frac 950 10 User Manual 18 1139 56 Edition AE Installation 2 3 Assembling tube racks There are four standard mode racks and two prep mode racks available as shown in the following table Each rack type has its own logical rack definition in UNICORN teen ation Pack type Color Tube combination whe heni S Standard mode A 18 and 30 mm Yellow 12x10 pos 18 mm tubes 120 115 mm tubes 2x4 pos 30 mm tubes 8 130 mm 12 mm tubes Violet 16x15 pos 12 mm tubes 240 100 mm C Microplates 96 and Blue 4x96 well microplates 384 50 mm 30 mm tubes 2x4 pos 30 mm tubes 8 136 mm 30 mm tubes Red 45 pos 30 mm tubes 45 115 mm Prep mode E 30 mm tubes Yellow 80 pos 30 mm tubes 80 115 mm 250 ml botiles Green 20 pos 250 ml bottles 20 180mm G 30 mm funnels Yellow 30 pos 30 mm funnels 30 funnel to flask 1 The following manufacturers microplates are tested and approved by Amersham Biosciences for use with this rack type Greiner low 655101 651101 650101 Greiner high 780201 Nunc low 143761 168055 156545 163320 The difference between these microplates is the b
7. moves rapidly Several beeps are heard before the calibration procedure starts WARNING Be sure to fold down the safety bar whenever the rack holder is operated by hand This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced CAUTION The DropSync unit will be damaged if it is positioned below the tube rims Switch on Frac 950 at the mains switch on the rear panel At switch on Frac 950 performs a self test There are green and yellow indicators on the front of Frac 950 The green indicator shows e when blinking power is on e when continuously lit power is on and connection with UNICORN is established When lit the yellow indicator shows that Frac 950 is running When connected to UNICORN several beeps are heard after which Frac 950 starts to perform an automatic calibration process During this process the tube holder moves rapidly in several directions When calibration is completed the tube holder stops in the home position Note The safety bar must be folded up for Frac 950 to start up Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Operation 3 2 Operating Frac 950 Frac 950 is controlled from a PC running UNICORN version 3 21 for standard mode or version 4 0 or higher for prep mode Control of Frac 950 can be achieved automatically from a method or manually via the functions available in UNICORN Using Frac 950 in a method
8. replaced follow the instructions in steps 3 and 4 Figure 2 11 Connecting a capillary to the upper port Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 21 22 Installation Use the standard AKTA connector on the DropSync unit to connect the free end of the capillary as shown in Figure 2 9 Loosen the knurled screw to lower the DropSync unit to improve access Thread the capillary end through the DropSync unit and the finger tight connector Allow the capillary end to stick out approximately 2 mm and tighten with your fingers only The capillary end should be straight and cleanly cut Adjust the height of the DropSync unit to suit the height of the tubes to be used Fasten the knurled screw Set operation parameters as follows e Go to System Settings Specials in UNICORN WINCORN_LAB2_NT Specials Instructions x m Instructions m FracParameters Parameters E Dele FracParameters DelayVol 0 10 DelayVol 0 375 ml 0 375 12 o TubeChange DropSync E E ml Specials Peak FracParameters StartSlope 100 000 m U min TubeChange e P EndSlope 75 000 m U min A lat Monitors Miriwidth 0 150 min Accumulator E Mode Open DropSync Accumulator C Curves Le Set Selected Parameter To Strategy Default Value Cancel Help e In FracParameters change the DelayVol parameter The AKTAdesign Optional Configurations User Manual for each KTAdesign system describes how to calculat
9. the chromatographic system outlet Use a standard AKTA connector Tighten with your fingers only Note For AKTAexplorer and AKTApurifier this is port 2 on the outlet valve For AKTAbasic and AKTAFPLC in standard config uration this is the outlet from the Flow restrictor FR 904 Use the standard AKTA connector on the DropSync unit to connect the free end of the capillary to Frac 950 as shown in Figure 2 9 Loosen the knurled screw to lower the DropSync unit to improve access Thread the capillary end through the DropSync unit and the finger tight connector Allow the capillary end to stick out approximately 2 mm indicated on the DropSync unit plastic housing and tighten with your fingers only The capillary end should be straight and cleanly cut Adjust the height of the DropSync unit to suit the height of the tubes to be used Fasten the knurled screw 19 Installation Capillary Knurled screw Finger tight connector Capillary end projection 2mm Capillary projection indication on DropSync unit Figure 2 9 Installing a capillary with DropSync CAUTION The DropSync unit will be damaged if it is positioned below the tube rims 6 Set operation parameters as follows e Go to System Settings Specials in UNICORN m Instructions m FracParameters Parameters lame FracParameters DelayVol 0 10 Delayvol 0 375 ml 0 375 E f TubeChange DropSync ml Specials Peak
10. the keying guide on the holder to position it correctly see Figure 2 8 Turn the rack on the holder until it snaps firmly in position Note The rack is kept in place on the holder with magnetic force Figure 2 8 Fitting the rack to the rack holder 3 Check that the tube numbering text on the tube holder is readable from left down 4 Insert tubes gently in the tube holder as desired making sure the tubes are inserted completely 5 Fold up the safety bar Installing a prep mode tube rack Note Installing the prep mode racks requires a Prep mode conversion kit See 2 11 Changing from standard mode to prep mode Fraction Collector Frac 950 18 User Manual 18 1139 56 Edition AE 2 5 Installation Installing capillaries General Frac 950 is delivered with capillaries to suit all AKTAdesign systems Select the capillaries to suit your AKTAdesign system Tubing i d mm 0 25 1 00 AKTAdesign system AKTAexplorer 10 AKTApurifier 10 AKTAbasic 10 AKTAexplorer 10 100 AKTApurifier 10 100 AKTAbasic 10 100 AKTAFPLC AKTAexplorer 100 AKTApurifier 100 KTAbasic 100 AKTAFPLC AKTAexplorer 100 Material color PEEK Blue PEEK Orange PEEK Green PEEK Beige The capillaries are fitted with standard AKTA finger tight connectors code no 18 1112 55 Using Frac 950 with DropSync To install the capillary 1 Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Connect a capillary from
11. the rack holder 7 29 Installation 30 Installing the Prep Mode Conversion kit ee 1 3 Retaining screw PAR rotary shaft Keying guide Positioning arm Figure 2 18 nstalling the dispenser shaft Install the dispenser shaft as shown in Figure 2 18 1 Fit the dispenser shaft to the guide pin on the positioning arm 1 using the keying guide to position it correctly 2 Turn the shaft on the guide pin until it snaps firmly in position 2 Insert and tighten the shaft retaining screw 3 See Assembling prep mode racks on page 17 for details of assembling the racks Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Installation Install a prep mode rack as shown in Figure 2 19 2 Make sure that the g guide pin fits Figure 2 19 nstalling a prep mode rack 1 Push the waste arm to the right 1 2 Place the rack on the Frac 950 2 Use the guide pins on the rack legs to position the rack Check that the guide pin fits 3 WARNING Moving parts The dispenser arm can make sudden rapid movements Do not have any part of your body within the unit base area when Frac 950 is in operation Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 31 Installation Fit the dispenser arm to t
12. 5 18 1109 74 18 1109 75 18 1117 74 gt DP gt DP D gt 1 A accessory C consumable Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Reference information C Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 57 58 C Reference information Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE June 2002 Short instructions The following short instructions are checklists for users who are fully familiar with the safety precautions and operating instructions described in this manual The instructions assume that optional equipment is installed according to the installation instructions Frac 950 is controlled from UNICORN For manual control use the instructions found in System Control Manual Frac 1 For collection of fixed fraction volumes select the instruction Man_Fractionation and enter the fraction size to start collection 2 For collection of peaks select the instruction Man_PeakFractionation and enter the peak size to start collection The liquid between the peaks will be diverted to waste if the instruction Man_Fractionation is not used at the same time 3 To stop collection select the instruction FractionationStop and or Peak_FracStop Amersham e Biosciences TC information ab Uppsala Printed in Sweden by T K i Uppsala AB
13. 8 1139 56 Edition AE Reference information A Reference information A Description A 1 Instrument Standard mode Prep mode Fraction Collector Frac 950 is an automated fraction collector for use in liquid chromatography as part of an AKTAdesign chromatography system The fractionation base can be selected as time or volume Collection vessel positions in a rack can be pre programmed using standard racks or occupy any position within the fractionation area new rack definition In standard mode which is the normal operational mode the capillary delivering the liquid is fixed and the rack holding the collecting vessels moves in an x y coordinate system to position the vessels under the capillary tip for collection In prep mode the opposite applies the rack holding the collecting vessels is fixed and the capillary delivering the liquid moves Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 51 A Reference information The moving pattern can be selected DE Fraction order to collect serpentine row row by row serpentine column or column Eid E by column c Bowby TD When Last tube is Defined and there row Sa are more fractions to collect an alarm is generated and the system is cc Serpentine paused column New empty tubes can then be o vray by inserted By using the instruction Ignore LastTube collecting ee f
14. E 43 Operation 3 8 AccumulatorWash 44 The accumulator used to eliminate spillage at tube change can be manually washed with the instruction AccumulatorWash 1 Start a flow of 10 ml min manually using the system pump 2 If the fraction collector is connected to any other port than port 1 in the Outlet valve you must manually switch Outlet Valve to the other port Select menu System Control Manual Flowpath in UNICORN 3 Select the instruction OutletValve and select the desired port 4 Click on the Execute button 5 Select menu System Control Manual Frac in UNICORN 6 Select the instruction AccumulatorWash in the Frac list r Instructi P ti 1 e ae IE en 1 10 Insert C Flowpath Man Peak Fractionation 5 M OFF Delete Alams Mon Peak Perdi G Frac Accumulator ash C ote TRE cones Help 7 Select the number of strokes to be used for washing with the parameter Strokes 8 Click on the Execute button Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Operation 3 9 Setting delay volume The delay volume between the UV flow cell in the chromatographic system and Frac 950 must be known to UNICORN The delay volume is used to adapt the collected fractions to the event marks generated by UNICORN 1 In UNICORN select menu System Control System Settings 2 Click the Specials radio button and select instruction FracParameters The DelayVol instruction becomes highlighted 3 To c
15. O AKTA design Fraction Collector Frac 950 User Manual Amersham 18 1139 56 R Biosciences Important user information All users must read this entire manual to fully understand the safe use of Frac 950 Amersham Biosciences AB which was previously known as Amersham Pharmacia Biotech AB changed its name to the current name on 17th October 2001 WARNING The WARNING sign highlights instructions A that must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood Caution The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets all requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked Amersham Biosciences instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from Amersham Biosciences except for alterations described in
16. Tube patterns as shown in UNICORN Method editor Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 15 Installation Assembling standard mode racks A complete standard mode rack model assembly consists of a bowl a tube support and a tube holder as shown in Figure 2 6 Tube holder with Tube support Bowl Figure 2 6 Rack model 18 and 30 mm tubes parts 1 Push the tube holder and tube support combination onto the bowl noting the keying guides on the bowl The surface of the holder should be level 2 All other standard mode rack models are assembled in a similar way Fraction Collector Frac 950 16 User Manual 18 1139 56 Edition AE Fraction Collector Frac 950 Installation Assembling prep mode racks A complete prep mode rack model assembly consists of a rack 4 rack legs and a bar Plug 3 2 N Phillips screw Rack legs Guide pi Note Place the legs with guide pins towards the side with the bulge Figure 2 7 Assembling Rack model 30 mm tubes Assemble the rack as shown in Figure 2 7 1 Fasten the two rack legs with guide pins to the bar using the supplied screws 1 2 Fasten all rack legs to the rack using the Phillips screws 2 Put the plugs in place 3 User Manual 18 1139 56 Edition AE 17 Installation 2 4 Installing a tube rack Installing a standard mode tube rack 1 Make sure the safety bar is folded down 2 Fit the rack to the rack holder using
17. _FracParameters StartSlope 100 000 m min TubeChange e P EndSlope 75 000 m4 amp U min A lat Monitors Miriwidth 0 150 min Accumulator Keyboard Mode Open C Curves k Set Selected Parameter To Strategy Default Value Cancel Help e In FracParameters change the DelayVol parameter The AKTAdesign Optional Configurations User Manual for each AKTAdesign system describes how to calculate the parameter values e Select Tube or DropSync as TubeChange parameter Fraction Collector Frac 950 20 User Manual 18 1139 56 Edition AE Installation Using Frac 950 with the accumulator 1 Connect a capillary from the chromatographic system outlet Use a standard AKTA connector Tighten with your fingers only Note For AKTAexplorer and AKTApurifier this is port 2 on the outlet valve For AKTAbasic and AKTAFPLC in standard configu ration this is the outlet from the Flow restrictor FR 904 2 Connect the free end of the capillary to the lower port on the valve manifold in the delivery unit on Frac 950 see Figure 2 10 Figure 2 10 Connecting a capillary to the lower port 3 Connect another capillary to the upper port on the valve manifold in the delivery unit on Frac 950 see Figure 2 11 Note A capillary running from the upper connector on the accumulator manifold to the DropSync unit is fitted at the factory This is a PEEK i d 0 75 mm green 250 mm long capillary If this capillary needs to be
18. consumables 56 Item Fraction collector Frac 950 complete with Rack A 18 mm 30 mm tube rack Rack A 18 mm 30 mm tubes complete with bowl tube support and tube holder Rack B 12 mm tubes complete with bowl tube support and tube holder Rack C microplates 30 mm tubes complete with bowl tube support and tube holder Rack D 30 mm tubes standard mode complete with bowl tube support and tube holder Rack E 30 mm tubes prep mode complete Rack F 250 ml bottles complete Rack G Funnel to flask kit complete with tubing funnels tubing guide and extension legs Prep mode conversion kit complete with shaft Safety bar with screws DropSync assembly complete PEEK tubing i d 0 25 mm o d 1 16 PEEK tubing i d 0 50 mm o d 1 16 PEEK tubing i d 0 75 mm o d 1 16 PEEK tubing i d 1 00 mm o d 1 16 Finger tight connector 1 16 Mains distribution cable for connection to AKTAdesign system Mains cable EU standard Mains cable US standard UniNet cable UniNet cable UniNet cable UniNet cable Quant pack 1 1 2m 2m 2m 10 tm 1 5m 0 7m 3m 15m A C Code no A 18 6083 00 A 18 6083 11 A 18 6083 12 A 18 6083 13 A 18 6083 14 A 18 6083 15 gt 18 6083 16 A 18 6083 17 gt 18 6083 18 18 6083 22 18 6083 23 18 1121 36 18 1113 68 18 1112 53 18 1115 83 18 1112 55 18 1032 08 gt O0O 00 0 0 S gt 19 2448 01 19 2447 01 18 1117 7
19. e the parameter value e Select Accumulator as TubeChange parameter Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Installation 2 6 Waste handling Fraction Collector Frac 950 1 Connect the supplied waste tubing to the waste outlet as shown in Figure 2 12 SS SH Connection adapter for waste tube Figure 2 12 Waste tubing connection 2 Place the free end of the waste tubing in a suitable container Note For AKTAbasic systems with no outlet valve and for AKTAFPLC this waste outlet is used for all liquid waste after the column All other system configurations use port F1 on the outlet valve for liquid waste after the column except for peak fractionation without ordinary fractionation during which the Frac 950 waste is used between peaks To empty the waste tube completely loosen the waste tube from the cable recess and lower it User Manual 18 1139 56 Edition AE 23 Installation 2 7 Connecting electrical signal cables At delivery the DropSync and the Accumulator cables are packed in a plastic bag See Figure 2 13 to locate the connectors on the rear panel of Frac 950 UniNet 1 connectors UniNet 3 connectors Mains switch Voltage Frequency 100 1207 50 60 Hz ol 9 220 240 v o Power max 300 VA DropSync Mains connector connector
20. ents 1 Introduction LL Generale niet nent nn vee sh Te aa aoa ES oE ounce Ida aaiae 7 1527 Safety sr es nee ese cede mn Reel ten EEL E eta rss ts DES 9 2 Installation QA Unpacking sms ovtivesitevestaciccs ensasscnsdesaacaueavausicatacvaresesavehacsianoed 10 2 2 General precautions unten nr szexeattasdavige decir e es inerte at 10 2 3 Assembling tube racks sisi ans denscsvsee ceive suas deoueesdeseesscneeseveecesedeens 11 Assembling standard mode racks cscccssccssccessesenseessesessceseceseceseeesseceecenseeeseees 16 Assembling prep mode racks ccecccccsssccsssscsessseeessessescecsseeesssecssseecsssaeessaeseees 17 DA Inistalling a tuberack Hesse tement st henri terre 18 Installing a standard mode tube rack vecccccccesssccecssceessccsesseesesscessseceseseeeessseeenss 18 Installing a prep mode tube rack viccccccccsscccssssceesssesessceseseessscesssseeeeseesessaeeeees 18 2 5 Installing capillaries ss me Mens aceoveoeud cb cattel sa entente min a es 19 General ss lesions aient else hae teste 19 Using Frac 950 with DropSync rennes 19 Using Frac 950 with the accumulator ss 21 2 6 Waste handling metier entier Sad nas ile dass 23 2 7 Connecting electrical signal cables eniste iai O 24 DropSynic connector ist EEE E E led lues 24 Accumulator connector serres 24 2 8 Connecting to a UniNet 1 communication link ceccccccesscesssseceeeeceeeseeeeeeees 25 2 9 Connecting to Supply Voltage reine r ee ER nn 26 2 10 Inserting collecti
21. er arm to suit the height of the tubes or the bottles to be used a distance between dispenser arm and the tip of the fractionation vessels of 0 5 1 cm is appropriate Tighten the knurled screw 4 Fold up the safety bar 33 Installation 2 12 Installing the Funnel to flask kit 1 Attach the extension legs to the rack model E See Assembling prep mode racks on page 17 2 Attach the Tubing guide to the rack as shown in Figure 2 22 Tubing guide Extended leg Tubing holder Figure 2 22 Attaching the Tubing guide 3 Install the rack Referring to Figure 2 19 on page 31 4 Cutthe tubing to appropriate lengths Fit the funnels to the tubings see Figure 2 23 Funnel Figure 2 23 Fitting a funnel to a tubing Fraction Collector Frac 950 34 User Manual 18 1139 56 Edition AE Installation 5 Feed the tubings with the funnels through the rack holes Organize the tubings at the tubing holders Make sure that the tubings are not squeezed Figure 2 24 Frac 950 with the Funnel to flask kit Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 35 Installation 2 13 Changing from prep mode to standard mode 36 Before changing the components CAUTION Before changing fractionation mode components the new waste position must be initialized in UNICORN Before changing the fractionation mode components initialize the new waste position of Frac 950
22. fold down the safety bar whenever the rack holder is operated by hand This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced CAUTION Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining the unit 5 1 Faults and actions If the suggested actions do not correct the fault call Amersham Biosciences Fault No tube change Tubes skipped DropSync is not functioning No fractions are collected Liquid misses the tubes Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Action 2 Start a flow and start fractionation Select FeedTube from the menu System Control Manual Flowpath If the motor does not start and an error appears call Amersham Biosciences Check the delay volume A large delay volume at a low flow generates a long delay time Faulty parameters in UNICORN may be the cause The drop sensor photocell is dirty Clean the photocell with a damp cloth Check that the capillary end projection is not too long 2 mm Check that the flow is not too high a continuous flow Check that the safety bar is folded up Check that the DropSync unit is close enough over the tubes Check that the rack is correctly fitted to the rack holder Check that the capillary end is cut cleanly and straight Check that the correct rack type is selected 49 Trouble shooting 50 Fraction Collector Frac 950 User Manual 1
23. hange the setting click on the up and down arrows for the DelayVol parameter or type a new value directly in the parameter window WINCORN LAB2 NT Specials Instructions x Instructions FracParameters Parameters Alama FracParameters DelayVol 0 10 TubeChange DropSync ml f Specials Peak FracParameters StartSlope 100 000 m amp U min TubeChange lei il EndSlope 75 000 m U min WEG ES Miriwidth 0 150 min DropSyme Keyboard Mode Open C Curves Set Selected Parameter To Strategy Default Value Cancel Help 4 The appropriate value for your AKTAdesign system is found in the AKTAdesign Optional Configuration User Manual 5 Click on the OK button The value entered will be used until a further change is made Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 45 Operation 3 10 Flow control during tube change The liquid flow during tube change can be handled in three different ways 1 In UNICORN select menu System Control System Settings 2 Click the Specials radio button and select instruction FracParameters Highlight the TubeChange instruction WINCORN_LAB2_NT Specials Instructions x m Instructions r FracParameters Parameters v FracParameters Delayvol 0 10 DelayVol 0 375 ml 0 375 4 TubeChange DropSyne Er ml Specials Peak FracParameters StartSlope 100 000 m amp U min TubeChange C EndSlope 75 000 mAU min E lat WEED MirWidth 0 150 min ov H Keyboard T
24. he prep mode 30 mm tube rack but only 30 of the 80 holes are filled with funnels 54 Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Reference information B B 3 Physical data Degree of protection IP 21 Wetted materials PEEK polyetheretherketone UHMW PE ultra high molecular weight polyethylene Glass Elgiloy HT Chemical resistance The wetted parts of the instrument are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 ethylacetate 100 hexane and 100 tetrahydrofuran THF Power requirement 100 120 220 240 V AC 50 60 Hz autorange switching Power consumption 300 VA Dimensions HxWxD 480 x 380 x 550 mm Weight 16 5 kg EMC Standards This product meets the requirement of the EMC Directive 89 336 EEC through the harmonized standard IEC EN 61326 1 emission and immunity Note The declaration of conformity is valid for the instrument when it is used in laboratory locations used in the same state as it was delivered from Amersham Biosciences except for alterations described in the user manual connected to other CE labelled Amersham Biosciences instruments or other products as recommended Safety Standards This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the harmonized standard EN 61010 1 Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 55 C Reference information C Accessories and
25. he shaft as shown in Figure 2 20 LE FZ shaft M View from above NJ Whee Figure 2 20 Mounting the dispenser arm 1 Loosen the knurled screw 1 2 Fit the tip of the knurled screw to the shaft recess 2 Tighten the knurled screw WARNING Moving parts The dispenser arm can make sudden rapid movements Do not have any part of your body within the unit base area when Frac 950 is in operation Fraction Collector Frac 950 32 User Manual 18 1139 56 Edition AE Installation Install the capillary as shown in Figure 2 21 Knurled screw 4 Finger tight ee connector 2 Swivel Capillary end projection 2mm Figure 2 21 Installing the capillary 1 Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Make sure that the capillary from the chromatographic system is connected to the lower port on the accumulator Connect the dispenser arm capillary i d 1 0 mm 800 mm to the upper port at the accumulator using the finger tight connector 1 Fit a finger tight connector to the swivel 2 Thread the free end of the capillary through the finger tight connector and the swivel Allow the capillary end to stick out approximately 1 2 mm 3 Hold the swivel with the 8 mm key and tighten the connector Adjust the height of the dispens
26. illage during fraction collection when the rack holder is moved by hand and when the rack is replaced WARNING The unit must not be opened by the user It contains high voltage circuits that can deliver a lethal electric shock CAUTION Always lift Frac 950 by the base unit NEVER by the safety bar delivery arm or rack holder as this may damage the unit CAUTION Only spare parts approved or supplied by Amersham Biosciences may be used for maintaining and servicing the unit CAUTION The DropSync unit will be damaged if it is positioned below the tube rims User Manual 18 1139 56 Edition AE 9 Installation 2 Installation 2 1 Unpacking Unpack the unit and check the items against the supplied packing list Inspect the items for obvious damage which may have occurred during transportation Retain all packing materials if onward transport of the unit is expected CAUTION Always lift Frac 950 by the base unit NEVER by the safety bar delivery arm or rack holder as this may damage the unit To make it easier to move Frac 950 on the laboratory bench first lift the front approx 30 and tilt the unit until the rubber feet clear the bench Then move the unit to the desired location 2 2 General precautions The unit should not be installed in a corrosive atmosphere The unit should be located in a place of low temperature variations away from heat sources draughts and
27. is described in the AKTAdesign Optional Configuration User Manual The following functions are available for operating Frac 950 from UNICORN Manual Method Man Fractionation Fractionation Fractionation Stop Fractionation Stop Feed Tube Feed Tube Man Peak Fractionation Peak Fractionation Peak Frac Stop Peak Frac Stop AccumulatorWash AccumulatorWash Ignore LastTube E Reset Frac Position Reset Frac Position PeakFrac Parameters PeakFrac Parameters Pa FractionationStop Pack nes Select rack types foret 18430 mmTubes x DEEE Man_Peak_Fractionation AlermskMon Peak FracStop Frac order Select fractionation order Peak Fact sameters Serpentinerow sa Ignore LastTube o Tube type Select tube type and siz mm H Frac size 0 000 99999 999 Select fractionation size D 0 000 Hm WINCORN_LABTANT Frac Instructions ix Instructions Parameters Pump Man Fractionation Start at ci Lima ji FractionationS toy Select starting tubes f RR AR fristtube Delete e Man Peak Fractionation F AlarmskMon Peak FracStop Base Select fractionation bases C Eac a E Time Volume Close Other Ignore_LastT ube zl Help It is also possible to set the delay volume i e the added volume of tubing and components between the UV flow cell in the AKTAdesign system and Frac 950 This value must be changed when the AKTAdesign standard configuration system is
28. l go The starting position can be freely selected This is stated in the method editor or manually in instruction Fractionation using parameter Start at Parameters Tube type rset 18mm E Peak_Fractionation Frac size 0 000 100000 000 Peak FracStop 0 000 E ml Change Peak_FracParameters Jef Accumulator Wash Stat at OutletFractions Fe g Replace Base O Time Volume Delete Ready I User Manual 18 1139 56 Edition AE 27 Installation 2 11 Changing from standard mode to prep mode General Using the Frac 950 in prep mode means that the rack is fixed and that the dispenser arm moves The prep mode is suitable for collecting extra large fraction volumes There are several accessories available for prep mode e The Prep mode conversion kit includes a rotary shaft with a dispenser arm This kit is necessary for using prep mode e The Rack E kit includes a 80 position rack for 30 mm tubes and 4 rack legs e The Rack F kit includes a 20 position rack for 250 ml bottles and 4 rack legs e The Rack G Funnel to flask kit includes 50 m silicone tubing 30 funnels 1 tubing guide and 4 extended rack legs Note Standard mode components must be removed before prep mode assembly Before changing the components CAUTION Before changing fractionation mode components the new waste position must be initialized in UNICORN Before changing the fractionation mode components initiali
29. n size Volume mode Time mode No spillage range Standard mode u titer plates DropSync 12 mm rack DropSync 18 and 30 mm racks DropSync Accumulator Prep mode 30 mm rack Accumulator Funnel to flask Accumulator 250 ml bottles Accumulator Environment Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 100 ml min 1 to 13 1 to 14 lt 1 day exposure 0 1 99999 99 ml 0 1 99999 99 min 0 1 0 ml min 0 1 5 ml min 0 2 0 ml min 15 100 ml min 15 100 ml min 15 100 ml min 15 100 ml min 4 to 40 20 95 relative humidity 84 106 kPa 840 1060 mbar atmospheric pressure 53 B Reference information B 2 Tube racks Rack designation and color Tube combination Laces Standard mode Rack A yellow 12x10 pos 18 mm tubes 120 2x4 pos 30 mm tubes 8 Rack B violet 16x15 pos 12 mm tubes 240 Rack C blue 4x96 well microplates 384 2x4 pos 30 mm tubes 8 Rack D red 45 pos 30 mm tubes 45 Prep mode Rack E yellow 80 pos 30 mm tubes 80 Rack F yellow 20 pos 250 ml bottles 20 Rack G green 30 pos 30 mm funnels 30 1 The following manufacturer s microplates are tested and approved by Amersham Biosciences for use with this rack type Greiner low 655101 651101 650101 Greiner high 780201 Nunc low 143761 168055 156545 163320 The difference between these microplates is the bottom shape and that a lid is included in some cases All have 96 wells 2 The funnel to flask rack is actually t
30. ng the instrument Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Introduction 1 Introduction 1 1 General Fraction Collector Frac 950 Fraction Collector Frac 950 is an automated fraction collector for use in AKTA design chromatography systems It is intended to be operated as an integrated part of an AKTAdesign chromatography system running UNICORN software Version 3 21 or higher is needed for standard mode 4 0 or higher for prep mode Frac 950 is equipped with an accumulator to eliminate spillage at high flows A drop sensor that can be used to control tube change at low flows is also included Frac 950 is delivered with one standard mode rack Different types of racks accommodating different sizes and types of tubes are available as options see Section 2 3 for details Accessories for collecting extra large fraction volumes in prep mode are also available see Section 2 3 for details Figure 1 1 Fraction collector Frac 950 standard mode User Manual 18 1139 56 Edition AE 7 Introduction Figure 1 2 Fraction collector Frac 950 prep mode Frac 950 features e Collection of up to 392 fractions time or volume base fixed volume and or peak fractionation fixed capillary tip moving rack standard mode fixed rack moving capillary tip prep mode fractionation order row by row column by col
31. on tubes iscsssssseiitensesn ensen eeesnecneeidienmeneunsss 27 2 11 Changing from standard mode to prep mode oo eessecesseeneeeseceteceetecetecceseeeseees 28 G n ral squeuasstsma isiresaissa te tasas resida sai aan aa RaNTa caca S TERA panda TS 28 Before changing the components sssesssessesssessersessssessessessressessressersresseseessees 28 Removing standard mode components ccsccccssssccsesssceessecsessecsesseecssseesssasesssaes 29 Installing the Prep Mode Conversion kit ccsccccssssccsssccssssscsssscsesscessssceesseeeeees 30 2 12 Installing the Funnel to flask kit wo cccccecsssccssscceessecceescecesseccseaeeceseesesseeseeeees 34 2 13 Changing from prep mode to standard mode eeesecesseenceeteceeeceeteceteeeeseeesaees 36 Before changing the components ss 36 Removing prep mode components o ccesscccesscccssssccesscceesseccsssesessscecesseecssaeeeseaes 37 Installing standard mode components icscccccssscecssscesssceeesscesssssessesessesessneeeees 39 Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE v Contents Operation 31 Onloff sccceaiseccatiataniccessestacceiivesdesccawetavccecastasscasuasesscasuasovaceceunssavecesbaaubidcnsaaneaccts 40 9 27 Operating Frar Is searre earra EAA en tn ln anse 41 3 3 Collecting fixed fractions ns 42 3 4 Collecting peak fractions sise mie ivresse dent 42 BES Feed tube seasicccsssssccscaievscccssiocatccosvenneg A 42 3 6 Ignore Last ber nn nine nt A et dti 43 327 o Reset Prac Po
32. ottom shape and that a lid is included in some cases All have 96 wells 2 The funnel to flask rack is actually the prep mode 30 mm tube rack but only 30 of the 80 holes are filled with funnels Frac 950 is delivered with rack type 18 and 30 mm tubes The other rack models are available as accessories Note Installing prep mode racks requires a Prep mode conversion Rit see 2 11 Changing from standard mode to prep mode Figures 2 2 2 3 2 4 and 2 5 show the tube patterns for the seven different logical rack definitions available in UNICORN Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 11 Installation Rack A 18 and 30 mm tubes yellow mmTubes Rack B 12 mm tubes violet H 2mm Tubes Figure 2 2 Tube patterns as shown in UNICORN Method editor Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Installation Rack C Microplates 96 and 30 mm tubes blue Rack D 30 mm tubes red Figure 2 3 Tube patterns as shown in UNICORN Method editor Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Installation Rack E 30 mm tubes yellow The only available option for this rack type Figure 2 4 Tube patterns as shown in UNICORN Method editor Fraction Collector Frac 950 14 User Manual 18 1139 56 Edition AE Installation Rack G 30 mm funnels funnel to flask yellow FurneHo fiask P Figure 2 5
33. ractions can resume F Define Set default Frac 950 can be set up to handle Tube type spillage during tube change in three Ee E different ways Number of tubes Tube 20 20 e DropSync Accumulator Tube means changing tubes without taking spillage into account i e spillage will occur Accumulator means that liquid is stored in an accumulating reservoir during tube change The accumulator reservoir consists of a syringe with a controlled plunger and a manifold When tube change is ordered liquid is drawn into the syringe by the moving plunger to store the required amount of liquid during tube change depends on the actual flow rate in the chromatography system When a new tube is positioned under the capillary tip the accumulated volume is quickly emptied in the new tube by the plunger and the remaining fraction volume is delivered to the capillary tip This is repeated for every tube change during fractionation DropSync means that liquid is controlled on a drop by drop basis A drop sensor is positioned at the delivery unit outlet It senses the drops falling from the capillary tip Tube change is carried out directly when the last drop in a fraction has fallen This method is suitable for small fractions Fraction Collector Frac 950 52 User Manual 18 1139 56 Edition AE B Technical specifications Reference information B B 1 Operating data Maximum flow rate pH stability range Fractio
34. re fractions to collect an alarm is generated and the system is paused You can then fill up with new tubes and use the instruction Ignore LastTube 1 Select menu System Control Manual Frac in UNICORN 2 Select the instruction Ignore LastTube in the Frac list WINCORN LAB14NT Frac Instructions x p Instructions Parameters SE Man Fractionation Insert ractionationStop C Flowpath FeedTube Dea Man Peak Fractionation AlarmstMon Peak_FracStop Frac Peak_FracParameters Accumulatorwash Close Other deve Help 3 Click on the Execute button 4 Fill up the rack with new tubes 5 Click on the Continue button to restart the fractionation in the next tube 3 7 Reset_Frac_Position Fraction Collector Frac 950 The instruction Reset_Frac_Position resets the fraction collector This means that fractionation set to start at next position will start in the first position The instruction will reset next position for all tube types 1 Select menu System Control Manual Frac in UNICORN 2 Select the instruction Reset Frac Position in the Frac list WINCORN LAB14NT Frac Instructions x r Instructions Parameters ER FractionationStop Pump FeedTube C Flowpath Man Peak Fractionation Delete Peak_FracStop Alarms amp Mon Peak_FracParameters Frac Accumulatorwash Ignore LastT ube Close C Other Help 3 Click on the Execute button User Manual 18 1139 56 Edition A
35. sition sr rien ns M Met ie OOE e ASE un tetes es ee lea RL 43 3 8 Accumulator Wash seule nine stresse Teena 44 3 9 Setting delay volume sescsvicocsccsscccsaseaacssesscascsaasvecenscesbassnscdsaasenccessabeabceesbaceseccets 45 3 10 Flow control during tube change ss 46 3 11 Define rack and tube parameters ss 47 Maintenance 41 Periodic maintenance seria nine cedar ceeds tobe da oE Dea Deca cused Ea TENE aa 48 4 2 Gleaning and Checking si eseiessara sine nids cvveciesodeiesbeleveoasccessecnebend s 48 4 3 Changing Capillaries anses nement ENEKE 48 4A Changing waste tubing lt 5 ccsssccesecccssscstscsntesvescassvscssesheescescesesncevessctvesans sdentevssets 48 Trouble shooting S i Faults and Actions esmas cestesstesevsasestvcsees tintin tete ll 49 Reference information Vi WA D SCrIPHON inner net me chee ee nt na one ends cube ne Tha E Otan N re 51 Instruments es dd nbs stele Palas eso ua aiid a oi a EE EER 51 B Technical specifications i ccccccccsesaccsccccsacsvacccssestacccsssseeaccasavvsasccdansecascaeoveesvecent 53 Operating data seeds dada Asia ish as CA Guia peca 53 TUDE TACRS is lt nm Us Tala oe ask ia ada ne ir etes 54 Physical datation tnt trees oie s s dia costs deteste tente 55 C Accessories and consumables ss 56 Short instructions About this manual This manual comprises two parts a practical part sections 1 5 anda reference part sections A C Sections 1 5 contain the necessary information for operati
36. this manual WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Fraction Collector Frac 950 User Manual 18 1139 56 AE Terms and Conditions of Sale All goods and services are sold subject to the terms and conditions of sale of the company within the Amersham Biosciences group which supplies them A copy of these terms and conditions is available on request Should you have any comments on this product we will be pleased to receive them at Amersham Biosciences AB SE 751 84 Uppsala Sweden Trademarks Drop Design FPLC UNICORN and AKTA are trademarks of Amersham Biosciences Limited Amersham and Amersham Biosciences are trademarks of Amersham plc Office Addresses Amersham Biosciences AB SE 751 84 Uppsala Sweden Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany Amersham Biosciences K K Sanken Building 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Amersham Biosciences China Limited 13 F Tower I Ever Gain Plaza 88 Container Port Road Kwai Chung New Territories Hong Kong Copyright Amersham Biosciences AB 2002 All rights reserved Contents Cont
37. to be used must be set This is described in detail in the AKTAdesign Optional Configuration User Manual Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 47 Maintenance 4 Maintenance 4 1 Periodic maintenance Fraction collector Frac 950 requires no periodic maintenance 4 2 Cleaning and checking The fraction collector should be kept clean and spilled liquid should be wiped off before it dries The rack holder should be positioned over the centre and the safety bar should be folded down when the fraction collector is not in use When your AKTAdesign system is cleaned also clean the capillaries and the accumulator in Frac 950 with distilled water The instrument should be wiped regularly with a damp cloth Remember to wipe the DropSync unit photocell as well Allow the instrument to dry completely before use 4 3 Changing capillaries Change the capillaries when they show signs of leakage or wear sharp bending for example 4 4 Changing waste tubing Change the waste tubing when it shows signs of wear Fraction Collector Frac 950 48 User Manual 18 1139 56 Edition AE Trouble shooting 5 Trouble shooting WARNING Always disconnect the power supply before attempting to replace any item on the instrument during maintenance WARNING The instrument must not be opened by the user It contains high voltage circuits that can deliver a lethal electric shock WARNING Be sure to
38. ube Mode Open DropSyne C Curves ey Set Selected Parameter To Strategy Default Value Cancel Help 3 For the parameter TubeChange select one of the following options e Tube No synchronization of collection Spillage will occur between tubes e DropSync Tube change is synchronized to drop release to minimize spill age Use i d 0 75 mm tubing between the DropSync and the ac cumulator e Accumulator During tube change the flow is diverted to the accumulator which stores the liquid When the new tube is in position the liquid is pressed out rapidly for collection Fraction Collector Frac 950 46 User Manual 18 1139 56 Edition AE Operation 4 Flow limit recommendations for the different methods are given in the following tables Standard mode Rack type Method Ed ad E for u titer DropSync 0 1 0 12 mm DropSync 0 1 5 18and30mm DropSync 0 2 0 18and30mm Accumulator 15 100 Note 18 and 30 mm racks At 2 15 ml min the liquid spillage is reduced by using the accumulator Prep mode Flow limit recommendation for Rack type Method no spillage ml min 30 mm Accumulator 15 100 Funnel to flask Accumulator 15 100 250 ml bottles Accumulator 15 100 Note At 0 5 15 ml min the liquid spillage is reduced by using the accumulator 5 After selection click on the OK button 3 11 Define rack and tube parameters When running a method the rack and tube parameters for the rack
39. umn serpentine row serpentine column e Choice of six different collecting racks four for standard mode collection and two for preparative prep mode One 18 30 mm tube rack for standard mode collection is supplied with Frac 950 The others are optional accessories e Two different methods for reducing spillage during tube change DropSync or Accumulator Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE 1 2 Safety Introduction A Pinch Hazard Fraction Collector Frac 950 The unit is designed for indoor use only e Do not use in a dusty atmosphere or close to spraying water Operate in accordance with local safety instructions WARNING Pinch hazard Do not have any part of your body within the unit base area when Frac 950 is switched on An automatic calibration process starts when Frac 950 is connected to UNICORN During calibration the dispenser arm moves rapidly Several beeps are heard before the calibration procedure starts WARNING Be sure to fold down the safety bar whenever the rack holder is operated by hand This blocks the rack holder from accidentally moving while the rack is moved by hand or replaced WARNING The unit must be connected to a grounded mains socket WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken WARNING When using hazardous chemicals take care to avoid sp
40. ze the new waste position of Frac 950 as follows 1 In UNICORN start the pump manually 2 In System Control select Manual Frac Select Man_Fractionation for the new mode See also Section 3 2 Operating Frac 950 3 Stop the run immediately when the first tube shift has been reported by clicking on the END button in the toolbar 4 Disconnect the system in UNICORN 5 Switch off Frac 950 at the mains switch on the rear panel 6 Switch on Frac 950 7 Connect the system in UNICORN When the system has connected to the Frac 950 again it should be positioned at the waste position of the new mode Fraction Collector Frac 950 28 User Manual 18 1139 56 Edition AE Installation Removing standard mode components Rack holder Safety bar Delivery arm Rack holder lid 5 i lt E 1 Rack holder retaining screw 7 Figure 2 17 Removing standard mode components Remove standard mode components as shown in Figure 2 17 1 2 N A a Fraction Collector Frac 950 User Manual 18 1139 56 Edition AE Fold down the safety bar 1 Remove the delivery arm lid 2 Loosen the Phillips screw 3 Place the delivery arm in prep mode position 4 Lift approx 0 5 cm and turn the delivery arm backwards until it snaps firmly in position Tighten the Phillips screw Fit the delivery arm lid Remove the rack holder lid 5 Loosen and remove the rack holder retaining screw 6 Remove

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