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HS-XX-Stim Users Manual

1. Figure 5 10 HS 18 Stim HS 18 MM Stim Sequence Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 15 The sequence will run indefinitely A Stop Command can be sent to stop the sequence in its current configuration Select Remove All to remove the Run Command from the Command List In the Stop Section of the HS XX Stim Control Software select Add Command The Command List should now contain a single Stop Command Command List gt Command 0281 00000000000000000000 Figure 5 11 Command List Containing a Stop Command Select Output to Headstage and the Stop Command in the Command List will be sent to the HS XX Stim Once the HS XX Stim receives the Stop Command it will immediately stop the sequence in its current state meaning some of the LEDs on the HS XX Stim Test Board will still be illuminated A Reset Command can be sent to turn Off all the LEDs In the Reset Section of the HS XX Stim Control Software select Add Command The Command List should now contain a single Reset Command Command List Command O20000000000000000000000 Reset Command Figure 5 12 Command List Containing a Reset Command Select Output to Headstage and the Reset Command in the Command List will be sent to the HS XX Stim Once the HS XX Stim receives the Reset Command it will immediately stop the sequence and turn Off all the LEDs on the HS XX Stim Test Board More information on the Hardware and Software is provided in the follo
2. The HS XX Stim Control Software is separated into three columns It is designed to be used starting in the top left region and ending in the bottom right region Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 20 2 Neuralynx WS XA 5tm Control Fla Carnaction Software Speed Help Configuration Panel Pa kirac Fause Time 7 7 zaten Configuration be paid Land gina Daviea Type H S 36 Sum Y rari Aima RENA 9 Thre Link A A o 101 A E T Add Command Add Ci mand Stop Sytch Configuration Add Cofimand Ard Col mand Switch rebori Save SABC Configuration Th gt Conti S figurat Pattern Configuration Change Address Paien Save Palen Sie 2 Contac Mew Aodross 01 Select Firat S Configuration gt Brtn NG Select Corresponding Pause 1 el f zeri acond Swi 2 Sni ect ora SE o H Seed hrd Sl Hi Select Ora 50 Seed Fourth Svi SESESESSESSESESESSSSESSESSESHSE ee EEE arpa fs a eck Craco EAL ee pon E Figure 7 2 Software Workflow 7 1 Device Address Device Address Headstage Device Type HS 36 Stim Device Ladress 0x00 0x0F y 01 e Figure 7 3 Device Address This Section of the HS XX Stim Control Software allows you to configure what type of headstage you are using and the device s address 7 1 1 Instructions 1 Select the HS XX Stim Type you are using by clicking the arrow on the Headstage Device Type pull down menu There are three options HS 36
3. Connection Softw Load Configuration Save Configuration Figure 7 17 File gt Load Configuration This Load Operation in the HS XX Stim Control Software allows you to load a Command List from a Configuration File Once the Load Operation is completed the Command List will be populated 7 11 Connection gt Connect to Cheetah Heuralynx_H5_XXx_5tin Aa Software Spe Connect to Cheetah COM Port Mode k S Figure 7 18 Connect to Cheetah This Operation allows the HS XX Stim Control Software to connect to Cheetah using the Server Name described in Section 8 1 Direct Control Once a connection has been established the name of the drop down will change to Disconnect from Cheetah COM Port Mode is not available at this time and not discussed in this document 7 12Software Speed gt Fast Slow Mode Software Speed w Fast Mode Slow Mode Figure 7 19 Fast Mode This Operation changes the speed at which the commands in the Command List are sent to the HS XX Stim Fast Mode sends all the commands to the HX XX Stim without expecting individual responses to each command The following figure 1s an example of the HS Stim Log File Output using Fast Mode E HS Stim Log File txt Notepad File Edit Format View Help Neuralynx HS Stim Log File Generated on March 26 2013 1 28 42 PM A HS xxX Stim Control software Version 1 0 1 Alpha2 Build Number 2013032640715 z operating system Microsoft windows xP version 5 1 Buil
4. 14 Select Connect to connect to Cheetah From this point forward and event matching the Event configured in Event Responder will send the Run Command to the HS XX Stim Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 37 9 Glossary Configuration File File containing HS XX Stim Commands Can be loaded in the Software or opened directly in Cheetah Device Address YC Address of the HS XX Stim If you are using multiple HS XX Stims a unique Device Address can be assigned to each HS XX Stim to communicate with each one individually EIB Neuralynx acronym for Electrode Interface Board HS XX Stim Command Packet sent to the HS XX Stim instructing it Ce Inter Integrated Circuit Communication Protocol Pattern Configuration Combination of Switch Configurations and Pause Times run sequentially Pattern Style 2 Configuration Combination of two Switch Configurations and two Pause Times run sequentially Pattern Style 3 Configuration Combination of three Switch Configurations and three Pause Times run sequentially Pattern Style 4 Configuration Combination of four Switch Configurations and four Pause Times run sequentially Pause Time Time specified between 0 0004 seconds and 6 5535 seconds for a Pattern Configuration Switch Configuration Definition of which switches on the HS XX Stim are On and which are Off Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 38
5. i Neuralynx High Density Electrophysiology J Recording Systems HS XX Stim Headstage which allows simultaneous stimulation and recording on electrodes Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 Fax 866 585 1743 www Neuralynx com Revision 1 5 support Neuralynx com 6 6 2013 Table of Contents L Document CIVIC Wissen sstite a eee as 5 2 HOAX SU OV as T TTT 5 3 What parts are needed to use the HS XX Stim sse 6 OR lt o Es 1 c 110 EPIA E nieces 6 Sa ro PO raanshuctheca acne 6 S E lt K E HU E 6 T A O II nn se errrenent eee nranney terre 7 d Blectrostatic Sensitive Equipment idilio ardilla 7 4 Installing the Digital Lynx SX Firmware Update occcccccooonnccnnnncnononcnnnnnnnnnnnnananonnos 8 S e A A 8 E PP II II II a tad etees 8 A A A 11 OE TSAA SSe UP tidad ea de 11 A A E AE I TOE EA EETA EA 12 6 2 1 Boot Cheetah and HS XX Stim Control Software sss 12 022 Connec 0 e E heet ieee aaa ole 12 0 243 Load Provided Configuration Piles cli 13 0 24 Contioure HS Xs SUM iria 14 To Hardwire Da ssns PUN A 17 Tal HSA SUMS WII s Te S een e e a a E E E E 17 L2 HS AX SUN COMMUNIC AMON cris 17 o xtra Hardware Connections midi 17 TE FHSAA AAA IA 18 7 5 Overdriving the HS XX Stim Stimulation Voltage occccooonnnnononocnncnononnnnnnnos 19 S OL Ware OVETVIS Wisin 20 SL Device Addres S srl dio 21 Slds MSTRUCUONS a bet taladidos 21 Dz ROS iaa li 22 S L FM SUR
6. 0F Ao0o00000000000000 Remove 7 920051615261536154610164 Remove All Server Mame localhost Output to Headstage Figure 7 15 Command List This Section of the HS X X Stim Control Software lists all of the commands that have been generated It allows you to move and remove commands from the list as well as configure the HS XX Stim directly with the software through a NetCom Connection with Cheetah specified by Server Name instructions for doing this are contained in Section 8 1 Direct Control 7 10File gt Load Save Configuration Heuralynx_H5_A Softw Connection Load Configuration Save Configuration Figure 7 16 File gt Save Configuration The Save Operation in the HS XX Stim Control Software allows you to save the Command List to a Configuration File This Configuration File can be loaded back into the software later or opened in Cheetah Saved Configuration Files are text files meaning they can be modified as long as the syntax remains the same For example more information specific to the experiment and each command in the file can be included This is done by adding a description after a sign The sign signifies a comment to the software meaning the entire line is ignored In addition certain parts of several Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 31 Configuration Files can be merger together This is done be Copying parts from one file and Pasting them in another Neuralynx_H5_
7. 2 Click Add Command and a single Run Command will be added to the Command List using the using the Device Address Selection described in Section 7 1 Device Address 7 6 2 Command Description This command executes its corresponding Saved Switch Configuration or Saved Pattern Configuration Once the command 19 received it takes about 0 0004 seconds 400us for the switches to set 7 7 Stop Stop Add Command Figure 7 13 Stop This Section of the HS XX Stim Control Software allows you to add a Stop Command to the Command List The command is generated using the Device Address Selection described in Section 7 1 Device Address 7 7 1 Instructions 1 Click Add Command and a single Stop Command will be added to the Command List using the using the Device Address Selection described in Section 7 1 Device Address 7 7 2 Command Description This command halts any executing Pattern Configuration immediately The HS XX Stim is left in its current configuration Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 29 7 8 Change Address Change Address Mev Address 01 had Force NO had Add Command Figure 7 14 Change Address This Section of the HS XX Stim Control Software allows you to add a Change Address Command to the Command List The command is generated using the Device Address Selection described in Section 7 1 Device Address or a Force Change Address Command can be used 7 8 1 Instructions 1 Select the New Address fo
8. 809 PM successful Reply Line 1 Hsstimcommand 0 OFAO0O0000000000000000 sending command sendLynxsxCcommand Acqsysteml Hsstimcommand 020C51615261536154610164 024C00000000000000000000 030000000000000000000000 sent 1 30 29 949 PM successful Reply Line 1 Hsstimcommand 0 51615261536154610164 sending command sendLynxsxcommand Acqsysteml HSstimcommand 025C00000000000000000000 Sent 1 30 30 059 PM successful Reply Line 1 Hsstimcommand 0 Completed on March 26 2013 1 30 30 PM lt Figure 7 22 Slow Mode HS Stim Log File Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 33 8 Software Operating Modes 8 1 Direct Control Operation 8 1 1 Description Direct Control Operation allows the HS XX Stim Control Software to communicate directly with the HS XX Stim The Output to Headstage button immediately sends each command from the Command List in Section 7 9 to the HS XX Stim s individually The software must be connected to Cheetah before clicking Output to Headstage for Direct Control to operate correctly The typical work flow of Direct Control Operation 1s illustrated in the figure below Build ammang Connect to Configure Boot Software gt List or Load gt Cheetah ed Headstage Configuration File Software NetCom Cheetah Fiber Digital Lynx SX 120 HS XX Stim Fi
9. Stim HS 18 Stim and HS 18 MM Stim 2 Select the Address of the HS XX Stim by clicking the arrow on the Device Address pull down menu There are 16 different address options Remember this 1s the actual Address of the HS XX Stim not what you want to the Address to be Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 21 Changing the HS XX Stim Address is described in Section 7 8 Change Address All HS XX Stims are shipped with 01 set as their default address 7 2 Reset Reset Add Command Figure 7 4 Reset This Section of the HS XX Stim Control Software allows you to add a Reset Command to the Command List The command is generated using the Device Address Selection described in Section 7 1 Device Address 7 2 1 Instructions 1 Click Add Command and a single Reset Command will be added to the Command List using the using the Device Address Selection described in Section 7 1 Device Address 7 2 2 Command Description The command turns OFF all switches on the HS XX Stim It can be sent at any time It can be used in two ways it can turn OFF all switches or stop a running Pattern Style Configuration and turn OFF all switches Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 22 7 3 Switch Configuration Swich Configuration Swich Configuration Save Switch Configuration 1 Y 7 3 1 l Ze Revision 1 5 6 6 2013 AD Channel U AD Channel 1 AD Channel 2 AD Channel 3 AD Channel 4 AD Channel 5 AD Channel 6 AD Channel
10. T AD Channel 2 AD Channel 9 AD Channel 10 AD Channel 11 AD Channel 12 AD Channel 13 AD Channel 14 AD Channel 15 AD Channel 16 AD Channel 17 AD Channel 13 AD Channel 19 AD Channel 20 AD Channel 21 AD Channel 22 AD Channel 23 AD Channel 24 AD Channel 25 AD Channel 26 AD Channel 27 AD Channel 28 AD Channel 29 AD Channel 30 AD Channel 31 Reference 1 Reference Reference 3 Reference 4 AD ChanelReference Switch Select OTT bad Stim Source 1 OTT bad Stim Source 2 Add Command Figure 7 5 Switch Configuration This Section of the HS X X Stim Control Software allows you to add a Save Switch Configuration Command to the Command List The command is generated using the Device Address Selection described in Section 7 1 Device Address Instructions Select the Switch Configuration number you want to save by clicking the arrow on the Switch Configuration pull down menu There are five options Select the Channel s Reference s in the list box you want connected to Stim HS XX Stim Users Manual Page 23 3 Connect these Channel s Reference s to Stim Source 1 by clicking the arrow next to the AD Channel Reference Switch Select pull down menu and selecting Stim Source 1 4 Select the Channel s Reference s in the list box you want connected to Stim Source 2 5 Connect these Channel s Reference s to Stim Source 2 by clicking the arrow next to the AD Channel Reference Switch Select pull down menu and sele
11. 010000 D Send Command Mode Slow Headstage 1 serial Number 0002 Headstage 1 Type 1810 Headstage 1 Date Code 11282012 Headstage 1 Software version 0000 Sending command sendLynxsxcommand Acqsysteml Hsstimcommand 020000000000000000000000 sent 1 30 29 184 PM successful Reply Line 1 Hsstimcommand 0 sending command sendLynxsxcommand Acqsysteml HSStimCommand 020100000000F00080700000 024100000000000000000000 030000000000000000000000 Sent 1 30 29 309 Pm successful Reply Line 1 HsstimcCommand 0 00000000FO0080700000 sending command sendLynxsxcommand Acqsysteml HSStimCommand 0202000000000000800000F0 024200000000000000000000 030000000000000000000000 Sent 1 30 29 434 PM Successful Reply Line 1 Hsstimcommand O 000000000000800000FO Sending command sendlynxsxcommand Acqsysteml HSStimCommand 02030000000000008000F000 024300000000000000000000 030000000000000000000000 sent 1 30 29 559 PM successful Reply Line 1 Hsstimcommand 0 0000000000008000F000 sending command sendLynxsxcommand Acqsysteml Hsstimcommand 02040000000000F080000000 024400000000000000000000 030000000000000000000000 sent 1 30 29 699 PM successful Reply Line 1 Hsstimcommand 0 0000000000FOSO0O000O sending command SendLynxsxcommand Acqsysteml Hsstimcommand 02110FA00000000000000000 02B100000000000000000000 030000000000000000000000 sent 1 30 29
12. 053140932 H Digital Lynx sx Version GetHardwareversion 0 DLSXMB_RO_BIT 1 020000 DLS MB_RO_SREC 1 020000 DLS MB_R1_BIT 1 010000 D Send Command Mode Fast Sending command sendLynxsxcommand Acqsysteml Hsstimcommand 020000000000000000000000 O201L00000000F 00080700000 0202000000000000800000F0 sent 1 28 42 543 PM successful Reply Line 1 Hsstimcommand 0 Completed on March 26 2013 1 28 42 PM i Figure 5 7 Log File You will notice that none of the LEDs on the HS XX Stim Test Board have illuminated This is because the Configuration File only contained Save Commands To initiate the saved pattern a Run Command needs to be sent to the HS XX Stim Select Remove All to remove all the Save Commands from the Command List In the Run Section of the HS XX Stim Control Software select Run Pattern Style 4 Configuration 1 from the Switch Configuration of Pattern pull down menu and select Add Command The Command List should now contain a single Run Command Command List _ _ Command 025c00000000000000000000 Run Pattern Style 4 Configuration 1 Command Figure 5 8 Command List Containing a Run Command Select Output to Headstage and the Run Command in the Command List will be sent to the HS XX Stim Once the HS XX Stim receives the Run Command it will immediately Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 14 start illuminating the LEDs on the HS XX Stim Test Board The sequence is illustrated below
13. Com Router provided by Neuralynx 3 Boot the HS XX Stim Control Software 4 Build your unique Command List or load it from a Configuration File Command Lists typically contain the following a Save Switch Configuration Commands b Save Pause Time Commands c Save Pattern Configuration Commands 5 Enter the Server Name in the Server Name text box Refer to Section 7 9 Command List 6 Select Cheetah Connection gt Connect to Cheetah It may take up to 30 seconds to connect to Cheetah The pull down will change to Disconnect from Cheetah when a connection has been established Refer to Section 7 11 Connection gt Connect to Cheetah 7 Click Output to Headstage and each command in the Command List will be sent to the HS XX Stim 8 When the log file is opened verify each command was sent successfully 9 Create a second command list consisting only of the Run Command for the Switch Configuration and Pattern Configuration you wish to run Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 36 10 Select File gt Save Configuration Enter the desired name and location of the Configuration File and save it Refer to Section 7 10 File gt Load Save Configuration 11 Boot Event Responder provided by Neuralynx 12 Select Add Event and configure the event you want to trigger the HS XX Stim 13 Select Add Response double click New Event Response and copy paste the entire Run Command from the saved Configuration File to Event Responder
14. Configuration Command 3 10202000000FOS00000FO0000 Save Switch Configuration 2 Command 7 0203F000000080000000F000 Save Switch Configuration 3 Command 5 020400F000008050000000F0 Save Switch Configuration 4 Command 6 02110F Ao0000000000000000 Save Pause Time 1 Command 7 020C051615261536154610164 Save Pattern Style 4 Configuration 1 Command Figure 5 6 Command List Containing Save Commands Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 13 The Command List now contains one Reset Command four Save Switch Configuration Commands one Save Pause Time Command and one Save Pattern Configuration Command 5 2 4 Configure HS XX Stim Once the Hardware is setup the HS X X Stim Control Software is connected to Cheetah the Configuration File has been loaded and HS Power is Enabled Cheetah 19 Acquiring you can now configure the HS XX Stim Select Output to Headstage and each command in the Command List will be sent to the HS XX Stim Once all commands have been sent a log file called HS Stim Log File txt will open and display the results It is overwritten each time Output to Headstage 1s selected E HS Stim Log File txt Notepad File Edit Format View Help Neuralynx HS Stim Log File Generated on March 26 2013 1 28 42 Pm HS xx Stim Control software Version 1 0 1 Alpha2 Build Number 2013032640715 O system Microsoft windows xP Version 5 1 Build 2600 service Pack 3 eetah software version 5 6 2 Build Number 2012
15. ICLIOIS na tad 22 5 22 Command Descrip od 22 89 Wienh Contorno 23 Sil SNS UPUICLIONIS a ad 23 32 Command DescapioM iaa lid EN 24 SA O a 25 Sd MSCONS ssgsveveusharins Radivestoabets 25 S42 Command Des cri pon ic ac etese rnd ccveseree E E Dedo bs 25 S AAA A 26 e AN A apes a vanmteee ea I neces sete 26 S22 Command DES CH pi ON sis cuseucenthone EEEE EA EDEA 2 A E E E E E 28 Sok MUU ON e e E sats 29 302 Command DescHpUON sea e 29 A a e TTT 29 A A AA a alban yard vootolnea S nacevad ods 29 22 Command Desceu ses ies sendin toed enc aa ele liado eras 29 Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 2 So Chis Address ee 30 S0 t L s c 5 Le 61575 it ees eee ee 30 5 02 Command DESCripiiOiic sic ieca licens eoutianric e waereteeiadulecs 30 S9 Command Litera ai 31 8 10 Pile Load Save STER ida 31 8 11 Connection gt Comectito 1 ad 32 8 12 Software speed gt Fast Slow Moders iioi 32 C Sottware Operatino Modest da 34 DL Direct C Onttor OC PEO lata 34 OAL R lt e cT s La crits Senet iss tence E N acids 34 OZ ASCRUC ROMS ais ai odiada 34 92 Conheuration Pile Operation tal 35 OL DESCAPION sa art e eS Gadasdete 35 O22 MASUNOS a ds 35 993 Realtime Operation Example oi id 36 IL DEC pi ios ee ds 36 OZ SUCIOS ni ida 36 EO e eT as ini A 38 List of Figures and Tables Figure 3 1 HS XX Stim and COmpOnentS sss see eee eee 6 Fiure 2 5 5 O Mis 6 rai 525 HS S A ccs dousesesanndadeatenade doaese 6 Figure S U
16. S MM SMa io 7 Figure 5 1 MATLAB Compiler Run Time Installer Splash Screen cooccccccncnnnnooo 8 Figure 5 2 MATLAB Compiler Run Time License Agreement sees sees eee eee 9 Figure 5 3 MATLAB Compiler Run Time Confirmation Page sss sees 9 Figure 5 4 MATLAB Compiler Run Time Installing ccccccccnonnonnonnncnnnnnnnnnnnnnnnnnnnnos 10 Figure 5 5 HS XX Stim Control Software eee 10 Figure 6 1 Hardware Connections erre 11 Figure 6 2 HS XX Stim Control Software eee eee eee eee 12 Figure 6 5 Connect to CCE Ca aci da 13 Figure 6 4 Disconnect Trond Cheetan ics vessseh lt xervsce censors an EEEE naveboncdedeaucucetweses 13 Ficure 6 5 File gt Lodd Confis urai ohn diia cion 13 Figure 6 6 Command List Containing Save Commands sss sss sese eee eee eee 13 ai T LOS TTT 14 Figure 6 8 Command List Containing a Run Command sss sees esse eee 14 PIS ube 6 9 H S 30 SUii 9 COU CIC sn Rca 15 Figure 6 10 HS 18 Stim HS 18 MM Stim Sequence sees eee eee eee eee 15 Figure 6 11 Command List Containing a Stop Command sese sese ee eee eee 16 Figure 6 12 Command List Containing a Reset Command esse sees eee 16 Fistire O H lt X SUN SWINE S idad loci 17 Figure 7 2 External Stimulation Connections cccccccccccccecceceeeeseeeeeeeeeceeceeeeeaaeeeeeeees 18 Figure 7 3 2 Pin BNC Pinout Connections sss sese e eee eee 18 Figure 7 4 SetPersistentHSPowerEnabled added to Cheetah cfg8 oooococcccccccnnnnnmmmmo 18 Revision 1 5 HS XX St
17. Style 3 Pattern Configuration 7 5 2 3 Save Pattern Style 4 Configuration 1 3 This command saves a unique Style 4 Pattern Configuration to the volatile memory in the HS XX Stim Up to three unique Style 4 Pattern Configurations can be saved Style 4 Pattern Configurations consists of a combination of four Switch Configurations four Pause Times a repeat value and a number of repeats value This is illustrated in the figure below Keep in mind this command only saves the Style 4 Pattern Configuration to n refer to Section 7 6 Run the HS XX Stim it does not actually start the patter k Q Q y y Y Z S S S Y 5 5 5 5 So So 20 Z g E S S E Q amp Z Z Z Z S Z O C o C E s o o Q 2 2 2 SOs E z 2 o a S OS 5 5 5 Z SZ G K K Ae Sw a Q Lime P Pausen P ause Time p ause Time E Use Time q Se Time y Figure 7 11 Style 4 Pattern Configuration 7 6 Run Run Swich Configuration or Pattern Run Switch Configuration 1 Add Command Figure 7 12 Run Revision 1 5 HS X X Stim Users Manual Page 28 6 6 2013 This Section of the HS XX Stim Control Software allows you to add a Run Command to the Command List The command is generated using the Device Address Selection described in Section 7 1 Device Address 7 6 1 Instructions 1 Select the Run Switch Configuration number Run Pattern Configuration number you want to save by clicking the arrow on the Switch Configuration or Pattern pull down menu There are fourteen options
18. Time Command will be added to the Command List using the using the Device Address Selection described in Section 7 1 Device Address 7 4 2 Command Description This command saves a unique Pause Time to the volatile memory in the HS XX Stim Up to five unique Pause Times can be saved Pause Times are used to configure Pattern Configurations Values range between 0 0004seconds 400us and 6 5535seconds 6553500us Keep in mind this command only saves a Pause Time to the HS XX Stim it does not actually pause the device Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 25 7 5 Pattern Configuration Pattern Configuration Pattern Save Pattern style 2 Configuration 1 a select First Switch Configuration Select Corresponding Pause Time select second Switch configuration 2 mul K Select Corresponding Pause Time Select Third Switch Configuration Select Corresponding Pause Time Select Fourth Switch Configuration Select Corresponding Pause Time Repeat Indefinth Mo Repeats 100 Add Command Figure 7 8 Pattern Configuration This Section of the HS XX Stim Control Software allows you to add a Save Pattern Configuration Command to the Command List The command is generated using the Device Address Selection described in Section 7 1 Device Address 7 5 1 Instructions 1 Select the Pattern Configuration Number you want to save by clicking the arrow on the Pattern pull down menu There are nine opti
19. Users Manual 6 6 2013 Page 11 4 Connect the Ribbon Cable to the 10 pin connector on the HS XX Stim Interface Board 5 Connect the other end of the Ribbon Cable to the 10 pin connector on the Global Reference Module Note The Ribbon Cable is a Daisy chain of up to nine connectors If you are using more than one HS XX Stim connect the ribbon cable to each Global Reference Module 6 Connect the HS XX Stim to HS XX Stim Test Board 5 2 Software 5 2 1 Boot Cheetah and HS XX Stim Control Software Power On the Digital Lynx SX and wait for boot cycle to complete On the Desktop select the Run Cheetah shortcut to open the Cheetah Welcome Screen Boot Cheetah with a configuration file for your setup Browse to the Directory the HS X X Stim Control Software was installed in and select Neuralynx_HS_XX_Stim Control exe Neuralynx_HS XX Stim Control File Connection Software Speed Help Configuration Panel Device Address Pause Time Run o Ne Switch Configuration or Pattern Headstage Device Type HS 36 Stim Pause Time Save Pause Time 1 Time Units l Run Switch Configuration 1 Device 4ddress 0x00 0x0F T 01 shes L _ Reset Maximum 6553500us Add Command 10000 us AS A Minimum 400us Add Command Switch Configuration Add Command Add Command Switch Configuration Save Switch Configuration 1 Y Pattern Configuration Pattern Save Pattern Style 2 Configurati
20. XX Stim The HS XX Stim requires the following items for use with the Digital Lynx SX and external stimulus device 1 HS XX Stim various models described in following sections HS XX Stim Interface Board Ribbon Cable Specific to SX HS XX Stim Test Board Two 2 Pin BNCs Blue Jumper a E Figure 2 1 If any item is missing or appears to be damaged please contact Neuralynx Support 2 1 HS 36 Stim e 32 AD Channels Unity Gain Amplification e 4 References Unity Gain Amplification e 2 Switches connected to each AD Channel and Reference e Master Control Switch for each Stimulation Source e Compatible with HS 36 Series EIBs e 4 0 grams 2 2 HS 18 Stim e 16 AD Channels Unity Gain Amplification e Reference Unity Gain Amplification e 2 Switches connected to each AD Channel and Reference e Master Control Switch for each Stimulation Source e Compatible with HS 18 Series EIBs Figure 2 3 HS 18 Stim e 4 3 grams Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 6 2 3 HS 18 MM Stim e 16 AD Channels Unity Gain Amplification e 1 Reference Unity Gain Amplification e 2 Switches connected to each AD Channel and Reference e Master Control Switch for each Stimulation Source e Compatible with Versa Drives e 4 3 grams Figure 2 4 HS 18 MM Stim 2 4 Electrostatic Sensitive Equipment All Neuralynx Equipment is Electrostatic Sensitive and should be handled with appropriate measures Alw
21. al connections that need to be made A HS XX Stim Interface Board with Ribbon Cable is provided for connecting the Digital Lynx SX Serial Device Expansion Port to the Global Reference Module on the DRS 36 Board This HS XX Stim Interface Board also provides access to Stim 2 Source Stim 2 Return and Panel Ground for connecting external Stimulation Sources using 2 Pin BNC Cables also provided by Neuralynx The connections on the HS XX Stim Interface Board for Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 17 connecting external Stimulation Sources are outlined in the figure below Notice the labels match those in the HS XX Stim Control Software J6 Figure 6 2 External Stimulation Connections The Pinout of a 2 Pin BNC Cable is shown in the figure below lt Signal lt Shield Figure 6 3 2 Pin BNC Pinout Connections 6 4 HS XX Stim Power All commands sent to the HS XX Stim are stored in Volatile Memory meaning that whenever the HS Power is disabled the HS XX Stim memory is reset A command can be added to the Cheetah cfg Configuration File to force HS Power to remain On The Command called SetPersistentHSPowerEnabled can be added below the ProcessConfigurationFile Command in Cheetah cfg as shown in the figure below P Cheetah cfg Notepad File Edit Format View Help eee eee ACQUISITION SYSTEM SETUP Do the basic setup for your acquisition hardware uncomment one only ProcesscontigurationFile Digitallynxsx
22. attern Save Pattern Style 2 Configuration New Address o S Select First Switch Configuration 4 Forces No Figure 4 5 HS XX Stim Control Software Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 10 5 Quick Start The following instructions are provided to quickly setup and test your HS XX Stim setup 5 1 HS XX Stim Setup Although the HS XX Stim connects to a Digital Lynx SX just like a standard Neuralynx Headstage there are some additional connections that need to be made A Control Cable is provided for connecting the Digital Lynx SX Serial Device Expansion Port to the Global Reference Module on the DRS 36 Board This cable also provides access to Stim 2 Source Stim 2 Return and Panel Ground for connecting to external Stimulation Sources The HS X X Stim connections are illustrated and described below C OO O0000 XS XUAT Jens Figure 5 1 Hardware Connections Connections 1 Connect the HS XX Stim to a DRS Board on the Digital Lynx SX a HS 36 Stim This requires a TETH HS 36 and an ADPT HS 36 DRS b HS 18 Stim HS 18 MM Stim This requires an ADPT HS 27 ERP 27 2 Connect the HS XX Stim Interface Board to the Serial Device Expansion Port on the Digital Lynx SX 3 Connect the Blue Jumper provided by Neuralynx to J6 on the HS XX Stim Interface Board This shorts 5 V to Stim Source 2 and should be removed when finished with the Quick Start Revision 1 5 HS XX Stim
23. ays wear a Static strap and use all appropriate ESD measures when handling any electronics Please contact Neuralynx for detailed information if you have questions Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 7 3 Installing the Digital Lynx SX Firmware Update Digital Lynx SX Firmware Update Version 1 20 is required to use the HS XX Stim The Firmware package can be downloaded from the Neuralynx Website www Neuralynx com gt Software gt Drivers and Firmware gt Digital Lynx SX Firmware Update Version 1 20 Instructions for installing the Firmware Update are contained in the download package 4 Installing the Software The HS XX Stim Control Software is compiled using the MATLAB Compiler Run Time Because of this the MATLAB Compiler Run Time must be installed on your system once to use the software A Package is provided by Neuralynx Package contains the MATLAB Compiler Run Time the Executable and some additional files The HS XX Stim Control Software can be installed and run on the same PC as Cheetah or on another network PC 4 1 Instructions 1 Save the HS_XX_Stim_Control_pkg exe file to a folder named HS XX Stim Control Software on the Desktop 2 Open HS_XX_Stim_Control_pkg exe if the Operating System asks for permission to open the file allow it Files will be extracted and saved in the folder This may take several minutes 3 The MATLAB Compiler Run Time Installer Splash Screen will appear MATLAB Compile
24. cfg ProcesscontigurationFile Digital yns cfg ProcessconfigurationFile SU p ProcessconfigurationFfile CcCheetahLled cfg ProcessconfiqurationF1le Sparrow cfg Processcontigurationri le RawDatafileFlayhack cfg 2 TPS Siti HS Pus YOXSXCOMMand Acaqsysteml SetPersistentHsPowerEnabledq i Figure 6 4 SetPersistentHSPowerEnabled added to Cheetah cfg The SetPersistentHSPowerEnabled command syntax is shown in the figure below It must be used with SendLynxSXCommand SendL ynxSX Command lt Hardware Sub System Name gt SetPersistentHSPowerEnabled lt Value gt Enables or Disables Persistent HS Power When enabled Persistent HS Power forces HS Power to remain On through events like stopping Cheetah Acquisition Example SendLynxSXCommand AcgSysteml SetPersistentHSPowerEnabled 1 Default Persistent HS Power is by default disabled Usage This Command can be used after the Digital Lynx SX Hardware Sub System has been created Arguments Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 18 Hardware Subsystem Name Name of sub system whose HS Power needs to be persistent This value can be one of the following keywords 1 Enables Persistent HS Power 0 Disables Persistent HS Power Figure 6 5 SetPersistentHSPowerEnabled Command Syntax 6 5 Overdriving the HS XX Stim Stimulation Voltage Warning Do not connect an external Stimulation Source to HS XX Stim Interface Board before HS Power has been enabled subj
25. cting Stim Source 2 6 All other unselected Channel s Reference s default to Off 7 Select what you want connected to Stim Source 1 by clicking the arrow on the Stim Source 1 pull down menu There are three options Off Stim 2 Source or Panel Ground 8 Select what you want connected to Stim Source 2 by clicking the arrow on the Stim Source 2 pull down menu There are three options Off Stim 2 Return or Panel Ground 9 Click Add Command and a single Save Switch Configuration Command will be added to the Command List using the using the Device Address Selection described in Section 7 1 Device Address 7 3 2 Command Description This command saves a unique Switch Configuration to the volatile memory in the HS XX Stim Up to five unique Switch Configurations can be saved A Switch Configuration specifies which switches on the HS X X Stim should be ON and which should be OFF Keep in mind this command only saves a Switch Configuration to the HS XX Stim it does not actually set the switches An illustration of what each major selection in this Section physically corresponds to on the HS X X Stim is shown in the figure below Switch Configuration Switch Configuration Seve Switch Configuration 1 7 AD ChannelReterence Switch Select AD Channel 1 AD Channel 2 AD Channel 3 AD Channel 4 AD Channel 5 AD Channel 6 AD Channel 26 AD Channel 27 AD Channel 28 AD Channel 29 AD Channel 30 AD Channel 31 Reference 1 R
26. d 2600 service Pack 3 eetah Software Version 5 6 2 Build Number 2012053140932 H Digital Lynx sx Version GetHardwareversion 0 DLSXMB_RO_BIT 1 020000 DLS MB_RO_SREC 1 020000 DLS MB_R1_BIT 1 010000 D Send Command Mode Fast Sending command sendLynxsxcommand Acqsysteml Hsstimcommand 020000000000000000000000 020100000000F00080700000 0202000000000000800000F0 sent 1 28 42 543 PM successful Reply Line 1 Hsstimcommand 0 Completed on March 26 2013 1 28 42 PM Figure 7 20 Fast Mode HS Stim Log File Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 32 Software Speed Fast Mode w Slow Mode Figure 7 21 Slow Mode Slow Mode sends each command to the HX XX Stim one at a time and queries the HS XX Stim to send each command back to the Software The following figure is an example of the HS Stim Log File Output using Slow Mode Notice the response to each command sent This is a good way to verify the communication with the HS XX Stim E HS Stim Log File txt Notepad SEE File Edit Format View Help Neuralynx Hs stim Log File Generated on March 26 2013 1 30 28 PM Hs xx stim Control software version 1 0 1 Alpha2 Build Number 2013032640715 operating system Microsoft windows xP Version 5 1 Build 2600 Service Pack 3 Cheetah Software Version 5 6 2 Build Number 2012053140932 Digital Lynx sx Version GetHardwareversion O DLSXMB_RO_BIT 1 020000 DLSXMB_RO_SREC 1 020000 DLSxMB_R1_BIT 1
27. ect safety will be lost Warning Do not allow the external Stimulation Sources to drive above the 5V Stimulation Voltage Range The HS XX Stim contains parts that protect it from damage if such an event occurs but subject safety and data integrity will be lost The following figure illustrates the crosstalk between an external Stimulation Source Yellow Trace and an Input Channel Blue Trace when the external Stimulation Source Voltage rises above 5V Fun Trig d E Moise Filter Off h 431137 j Figure 6 6 Overdriving the HS XX Stim Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 19 7 Software Overview The HS XX Stim Control Software user interface is shown in the figure below Descriptions of each section fellow 8 10 EE b Bortigueatione Sue SwetchContigurmicn 1 w Tare Palen Shee 7 CSS irs H al 3 Select First Sebch Configaraliorc 1 L HEH 3 Seleel Zed E Beh Cantiga Sead Comespordhg alee me HEHEHEH 3 Select Third Seteh Congar Select Conrespordngy aaa ime Sele Fourth Sua tch Coripe Lh Ch Ch Ch Eh Ch Ch Ch Eh Eh Ch Ch th Eh Ch Ch Ch th Ch Ch hi Eh th ch Ch En Eh Lh Ch th Stim Source 1 Select Comespondng TALEE ime FEE Ee ERUSENGVURY Pr eo ee 6666 LAT EF Stim Saee E _ J I Papes ndefinihi Ya A Repase 100 HL al if 1 SACO Ona SLE ME j Add Corrmand crit ka Boric Mi T les Figure 7 1 Software Sections
28. eference Reference 3 Reference 4 Stim Source 1 Off Stim Source 2 oft Add Command Electrode Connection Unity Gain Amplifier G 1 0 Buffered Signal at Digital Lynx SX Individual Channel Switch Stim 2 Source at Digital Lynx SX Stim Source 1 Panel Ground Master Control Switches Stim 2 Return at Digital Lynx SX Stim Source 2 Panel Ground Figure 7 6 Switch Configuration Illustration Revision 1 5 6 6 2013 HS XX Stim Users Manual Page 24 7 4 Pause Time Pause Tite o AnoOa aaa e Pause Time Save Pause Time 1 ha Time Units US ho Maximum 6553500us 10000 EE wlinimum 400s Add Command Figure 7 7 Pause Time This Section of the HS XX Stim Control Software allows you to add a Save Pause Time Command to the Command List The command is generated using the Device Address Selection described in Section 7 1 Device Address 7 4 1 Instructions 1 Select the Pause Time number you want to save by clicking the arrow on the Pause Time pull down menu There are five options 2 Time Units is provided for readability Select the preferred Time Units you want by clicking the arrow on the Time Units pull down menu There are three options us ms and s The Minimum and Maximum Time will automatically update when Time Units is changed 3 Enter a time between the listed Minimum and Maximum time values in the Pause Time text box 4 Click Add Command and a single Save Pause
29. elect File gt Open Configuration File Browse to the Configuration File and select Open 6 Each command in the Configuration File will be sent to the HS XX Stim If the Command List contains a Run Command it will immediately be executed A single Run Command can also be sent at a later time with a separate Configuration File Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 35 8 3 Closed Loop Operation Example 8 3 1 Description Closed Loop Operation allows the user to trigger stimulation based on a triggered event The typical work flow of Closed Loop Operation is illustrated in the figure below Cheetah Fiber Digital Lynx SX JCE HS XX Stim A Boot Software H gt 3 gt List or Load gt Build Command Conh ctto Gonna Event Responder J gt Trigger Run Cheetah Headstage Command Configuration File Software NetCom Cheetah Fiber Digital Lynx SX JCE HS XX Stim Figure 8 3 Closed Loop Operation Work Flow 8 3 2 Instructions Step by Step Instructions for using Closed Loop Operation using Event Responder and the NetCom Router are provided below It is important to remember that these are provided as a guideline l Prepare your recording session be configuring your hardware and Cheetah Start Acquisition in Cheetah 2 Boot the Net
30. gure 8 1 Direct Control Operation Work Flow 8 1 2 Instructions Step by Step Instructions for using Direction Control Operation are provided below It is important to remember that these are provided as a guideline 1 Prepare your recording session be configuring your hardware and Cheetah Start Acquisition in Cheetah 2 Boot the HS XX Stim Control Software 3 Build your unique Command List or load it from a Configuration File Command Lists typically contain the following a Save Switch Configuration Commands b Save Pause Time Commands c Save Pattern Configuration Commands d A single Run Command 4 Enter the Server Name in the Server Name text box Refer to Section 7 9 Command List 5 Select Cheetah Connection gt Connect to Cheetah It may take up to 30 seconds to connect to Cheetah The pull down will change to Disconnect from Cheetah when a connection has been established Refer to Section 7 11 Connection gt Connect to Cheetah 6 Click Output to Headstage and each command in the Command List will be sent to the HS XX Stim If the Command List contains a Run Command it will immediately be executed A single Run Command can also be sent at a later time 7 When the log file is opened verify each command was sent successfully Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 34 8 2 Configuration File Operation 8 2 1 Description Configuration File Operation allows the user to bypass the NetCom connection and co
31. im Users Manual 6 6 2013 Page 3 Figure 7 5 SetPersistentHSPowerEnabled Command Syntax sss sees 19 Figure 7 6 Overdriving the HS XX Stim apap eE RSE LES 19 Freure S d Software ECON Sist il a 20 Figure 2 SOW are W OL KEIO Wes E E esa eee 21 SE ET ICV ICC Addres dida 21 PT OUTC SARE E leri ida 22 PISUre 8 5 Witch C onn oura ON eia oia 23 Figure 8 6 Switch Configuration IllustratiON ooooooononnnnnnnnnnnnononononnnoncnnnnnnnnnnnnnonnnnnnos 24 SER s A Pause Ti oia 25 Figure TRR STS TT Te dde 26 Pioure o 9 Style 2 Pattern onfi gura ON ida 21 Figure LO Style 3 Pattern Conte Se e 28 Pioure B Style 4 Pattern Conti Te 28 Figure e RUN dd ea 28 BT OUT CeO SLOP da 29 Figure S 14 Mam Se Add a 30 Fiore s 15 C Omnia Sid 31 Freure 5 16 File gt Save CONS Ur AU OM cados 31 Froure 6 17 Pile gt Load Contisura On siii 32 Froure 5 13 Comnectto T E doi 32 Fours LVFS t Mod soii a2 Freure 5 20 Fast Mode HS Sm Los Pile cra aeon 32 Foures L VSO W MOE socia 33 Froure 5 22 Slow Mode HS S0m Los Ple doi 33 Figure 9 1 Direct Control Operation Work Flow ooooocccccccccccnnnnnnnnooncnnnnnnnnnnncnononnnnns 34 Figure 9 2 Configuration File Operation Work FloW ooccccccccccncnnnnnnooncnnccnnnnnnnonononannns 35 Figure 9 3 Real Time Operation Work PlOW coi a 36 Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 4 Document Overview This document describes the specifications and features of the HS XX Stim It also explains h
32. nel Device adios ELL Run E Switch Configuration or Pattern t v ao Dna TYEE HS 36 Stim Pause Time Save Pause Time 1 Time Units de E Run Switch Configuration 1 v Device Address 0x00 0x0F ot gt A Maximum 6553500us Add Command 10000 jus Minimum 400us Add Command Switch Configuration Switch Configuration Save Switch Configuration 1 Y COGE A AD ChannelReference dda Switch Select AD Channel 3 AD Channel 4 Off AD Channel 5 AD Channel 6 gt 3 AD Channel 7 Select Corresponding Pause Time AD Channel 9 q Add Command AD Channel 10 s S eed Select Second Switch Configuration AD Channel 13 2 Command List AD Channel 14 Select Corresponding Pause Time AD Channel 15 p 3 Command Move Up AD Channel 16 S AD Channel 17 Ch AD Channel 19 Select Third Switch Configuration AD Channel 20 3 on AD Channel 21 A TOAN ove Down AD Channel 22 Select Corresponding Pause Time AD Channel 23 AD Channel 24 AD Channel 25 AD Channel 26 Select Fourth Switch Configuration Remove AD Channel 27 4 AD Channel 28 Stim Source 1 l AD Channel 29 Select Corresponding Pause Time AD Channel 30 Off AD Channel 31 r s Remove All Reference 1 Reference 2 Stim Source 2 Repeat Indefinitly No Repeats 100 Referens 4 nd oft gt NS i Server Mame localhost Add Command Add Command Output to Headstage Add Command Add Command Pattern Configuration Change Address P
33. nfigure the HS XX Stim through a Configuration File s in Cheetah This is useful for setting up and performing consistent recording sessions Loading and Saving of Configuration Files is described in Section 7 10 File gt Load Save Configuration The typical work flow of Configuration File Operation is illustrated in the figure below Build Command Save Configure Boot Software gt m Configuration gt g List i Headstage File s Load Configuration l pi l L E Cheetah Fiber Digital Lynx SX 12C gt HS XX Stim File s in Cheetah Figure 8 2 Configuration File Operation Work Flow 8 2 2 Instructions Step by Step Instructions for using Configuration File Operation are provided below It is important to remember that these are provided as a guideline 1 Boot the HS XX Stim Control Software 2 Build your unique Command List Command Lists typically contain the following a Save Switch Configuration Commands b Save Pause Time Commands c Save Pattern Configuration Commands d A single Run Command 3 Select File gt Save Configuration Enter the desired name and location of the Configuration File and save it Refer to Section 7 10 File gt Load Save Configuration 4 Prepare your recording session be configuring your hardware and Cheetah Start Acquisition in Cheetah 5 In Cheetah s
34. on 1 y Change Address New Address 101 T Al AD ChannelReference AD Channel 2 Switch Select AD Channel 3 Off Seve J AD Channel 4 gt Select First Switch Configuration 1 Y l AD Channel 5 1 g Force No AD Channel 6 l l PS AD Channel 7 Select Corresponding Pause Time 1 Y AD Channel 8 AD Channel 9 ee Add Command AD Channel 10 AD Channel 11 E R P AD Channel 12 Select Second Switch Configuration AD Channel 13 2 C Command List AD Channel 14 Select Corresponding Pause Time AD Channel 15 p 9 M Command AD Channel 16 AD Channel 17 AD Channel 18 n 2 AD Channel 19 Select Third Switch Configuration AD Channel 20 a das A Select Corresponding Pause Time AD Channel 23 AD Channel 24 AD Channel 25 AD Channel 26 Select Fourth Switch Configuration AD Channel 27 3 AD Channel 28 Stim Source 1 AD Channel 29 P mn Select Corresponding Pause Time AD Channel 30 Off Y AD Channel 31 Reference 1 Reference 2 Remove All Stim Source 2 l Reference 3 a Repeat Indefinitly No Y Repeats 100 Reference 4 mM Off kd L 1 Add Command Add Command Output to Headstage Server Name localhost Figure 5 2 HS XX Stim Control Software 5 2 2 Connect to Cheetah The HS XX Stim Control Software must be connected to Cheetah through NetCom before configuring the Headstage To connect to Cheetah select Cheetah Connection gt Revision 1 5 HS XX S
35. ons The options are further described in Section 7 5 2 Command Description 2 Select the First Switch Configuration and Pause Time by clicking their pull down menu arrows These correspond to the Switch Configurations and Pause Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 26 Times you saved in Section 7 3 Switch Configuration and Section 7 4 Pause Time 3 Depending on the Pattern Style you selected select up to three more Switch Configurations and Pause Times by clicking their pull down menu arrows These correspond to the Switch Configurations and Pause Times you saved in Section 7 3 Switch Configuration and Section 7 4 Pause Time The software will automatically disable pull down menus that do not correspond to the selected Style 4 Select whether or not you want the Pattern to repeat indefinitely by clicking the arrow on the Repeat Indefinitely pull down menu The options are Yes or No 5 If you set the Repeat Indefinitely pull down menu to No enter the number of repeats in the Repeats text box Allowable value range from 1 to 255 6 Click Add Command and a single Save Pattern Configuration Command will be added to the Command List using the using the Device Address Selection described in Section 7 1 Device Address 7 5 2 Command Description 7 5 2 1 Save Pattern Style 2 Configuration 1 3 This command saves a unique Style 2 Pattern Configuration to the volatile memory in the HS XX Stim Up to three unique Style 2 Pa
36. ow to setup your headstage test it and use it during normal operation There 1s a glossary at the end of the document 1 HS XX Stim Overview This HS XX Stim is a headstage that not only records from electrodes but also allows stimulation of electrodes Each input on the HS X X Stim up to 36 electrode connections has two individual switches attached to it This allows for monopolar and bipolar stimulation protocols ie stimulation can be delivered from any electrode to a reference Panel Ground or between any number of electrodes Features Versions include HS 36 Stim HS 18 Stim and HS 18 MM Stim Exact same Low Noise Unity Gain Amplifiers G 1 0 as pervious headstages High Common Mode Rejection Ratio CMRR Two Individual Switches connected to each electrode One Master Control Switch for each stimulation source Two Way Communication between HS XX Stim Processor and Digital Lynx SX Command Builder Software provided Download Custom Switch Configurations and Patterns Stimulation times down to 400us with 100us resolution Up to 50mA per channel 5V Max Subject Safety and Data Integrity will be lost if exceeded Refer to Section 6 5 Overdriving the HS XX Stim Stimulation Voltage 21Q Typical Switch On Resistance NetCom Controllable Compatible with Neuralynx EIBs and Versa Drives Compatible with Digital Lynx SX systems Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 5 2 What parts are needed to use the HS
37. r Runtime Installer To install MATLAB Compiler Runtime 7 17 on your computer click Next a LA MATLAB MATLAB and Simulink are registered trademarks of The Mathworks Inc Please see R HUNTIN ms mathworks com trademarks For a list of additional trademarks Other product or brand R2012a names may be trademarks or registered trademarks of their respective holders WARNING This program is protected by copyright law and international treaties Copyright 1984 2012 The Mathworks Inc Protected by 11 5 and other patents See Mathworks com patents P MathWorks Figure 4 1 MATLAB Compiler Run Time Installer Splash Screen 4 Select Next gt to proceed to the License Agreement Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 8 Z License Agreement The Mathworks Inc MCR MATLAB COMPILER RUNTIME LIBRARIES LICENSE IMPORTANT NOTICE BY CLICKING THE YES BUTTON BELOW YOU ACCEPT THE TERMS OF THIS LICENSE IF YOU ARE NOT WILLING TO DO 50 SELECT THE NO BUTTON AND THE INSTALLATION WILL BE ABORTED 1 LICENSE GRANT Subject to the restrictions below The MathWorks Inc MathWorks hereby grants to you whether you are an individual or an entity a license to install and use the MATLAB Compiler Runtime Libraries MCR solely and expressly for the purpose of running software created with the MATLAB Compiler the Application Software and for no other purpose This license is personal nonexclusive and nontransPerable
38. r the HS XX Stim by clicking the arrow on the New Address pull down menu There 16 are different address options This 1s the New Address of the HS XX Stim not the current Address The Device Address Selection in Section 7 1 Device Address must be correct for this command to work properly If you do not know the current Address of the HS XX Stim refer to the next instruction step 2 If you do not know the current Address of the HS XX Stim set Force to Yes This will ignore the HS XX Stim s current Address and force it to the New Address Only one HS XX Stim should be connected to the system when using this feature 3 Click Add Command and a single Change Address Command will be added to the Command List using the using the Device Address Selection described in Section 7 1 Device Address 7 8 2 Command Description This command changes the HS XX Stim s Address This allows multiple HS XX Stims to operate on the same bus Please note that in order to change the address of the HS XX Stim you need to know its current Address If you do not know its current Address a Force can be used to update it Only one HS XX Stim should be connected to the system when using this feature Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 30 7 9 Command List Command List Command 020000000000000000000000 Z 02010000FOO0S0A0FODODODO 3 0202000000F0800000F00000 d 0203F000000080000000F000 Move Up Move Doyen 5 020400F000008050000000F0 6 0244
39. tim Users Manual 6 6 2013 Page 12 Connect to Cheetah also described in Section 7 11 Connection gt Connect to Cheetah The HS XX Stim Control Software will connect to Cheetah using the Server Name listed in the Command List Section of the software described in Section 7 9 Command List Heuralynx_H5_Xx_5tin Aa Software Spe Ci Connect to Cheetah or ode E COM Port Mod b Figure 5 3 Connect to Cheetah Once a connection has been established the pull down menu will change to Disconnect from Cheetah Neuralynx_H _XX_Stim_Cc Seem Software Speed C Disconnect From Cheetah COM Port Mode k L Figure 5 4 Disconnect from Cheetah 5 2 3 Load Provided Configuration File A Configuration File is provided with the HS XX Stim Control Software for testing the Hardware To load this Configuration File into the HS X X Stim Control Software select File gt Load Configuration File also described in Section 7 10 File gt Load Save Configuration Heuralynx_H5_X Connection SoFti Load Configuration Save Configuration Figure 5 5 File gt Load Configuration Depending on the HS XX Stim you are using browse to HS 36 Stim Quickstart cfg or HS 18 Stim Quickstart cfg in the HS XX Stim Control Software Folder on the Desktop and select Open The Command List will populate with commands from the Configuration File Command List Command o20000000000000000000000 Reset Command 2 02010000F0005040FODOO000 Save Switch
40. ttern Configurations can be saved Style 2 Pattern Configurations consists of a combination of two Switch Configurations two Pause Times a repeat value and a number of repeats value This is illustrated in the figure below Keep in mind this command only saves the Style 2 Pattern Configuration to the HS XX Stim it does not actually start the pattern refer to Section 7 6 Run 5 Ly S 5 E Q0 OD E E ES S S 5 Z S g 9 S Seg y D Ow 5 S se O 2 2 SOs E a a S e 8 5 lt ZE 5 0 hy Ar GC G d G 0 lt lt E Pause Tine E Pause Time F Figure 7 9 Style 2 Pattern Configuration 7 5 2 2 Save Pattern Style 3 Configuration 1 3 This command saves a unique Style 3 Pattern Configuration to the volatile memory in the HS XX Stim Up to three unique Style 3 Pattern Configurations can be saved Style 3 Pattern Configurations consists of a combination of three Switch Configurations three Pause Times a repeat value and a number of repeats value This is illustrated in the Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 27 figure below Keep in mind this command only saves the Style 3 Pattern Configuration to n refer to Section 7 6 Run the HS XX Stim it does not actually start the patter O Ka Q S y S S Ss Ss x E 2 amp y Ss S SS So So So 0 E S E y 2 z S Z Z S 2 O O O SA o Q Q O e 5 S S S S 30 2 2 OM amp L Q Q 5 G N y y ww O a Re Re Sn Q Time gt ause Time p ause Time G Figure 7 10
41. wing sections of this document Be sure to remove the Blue Jumper from the HS XX Stim Interface Board before continuing Revision 1 5 HS XX Stim Users Manual 6 6 2013 Page 16 6 Hardware Overview 6 1 HS XX Stim Switches Each AD Channel and Reference on the HS XX Stim is connected to two individual switches These switches allow any channel to be connected to up to two Stimulation Sources or Panel Ground Each switch is individually controllable Two master control switches control the stimulation sources This concept is illustrated in the figure below Buffered Signal at Digital Lynx SX Electrode Connection Unity Gain Amplifier G 1 0 Individual Channel o Switch o Stim 2 Source at Digital Lynx SX o Stim Source 1 C Panel Ground q _ __ a Master Control Switches o Stim 2 Return at Digital Lynx SX o Stim Source 2 gt Panel Ground o T Figure 6 1 HS XX Stim Switches 6 2 HS XX Stim Communication The HS XX Stim communicates with the Digital Lynx SX via an IC Bus The Digital Lynx SX is the Master and the HS XX Stim is the slave in the setup Communicating via PC allows multiple HS XX Stims on the same bus Packets sent to the HS XX Stim must follow a strict 12 byte format Neuralynx provides HS X X Stim Control Software for building and sending packets 6 3 Extra Hardware Connections Although the HS XX Stim connects to a Digital Lynx SX just like a standard Neuralynx Headstage there are some addition
42. z LICENSE RESTRICTIONS You shall not modify or adapt the MCR for any reason You shall not disassemble decompile or reverse engineer the MCR You shall not alter or remove any proprietary or other legal notices on or in copies of the Do you accept the terms of the license agreement No om MathWorks Figure 4 2 MATLAB Compiler Run Time License Agreement 5 When you have finished reading the License Agreement select Yes then Next gt 6 The next page is the Confirmation Page Select Install gt Confirmation E ES Confirm your installation settings Installation folder CA Program Files MATLAB MATLAB Compiler Runtimety717 One or more products are already installed in this Folder and will be overwritten Products MATLAB Compiler Runtime 7 17 MathWorks i K Figure 4 3 MATLAB Compiler Run Time Confirmation Page 7 The MATLAB Compiler Run Time will now begin installing This may take up to 15 minutes to complete Revision 1 5 HS X X Stim Users Manual 6 6 2013 Page 9 26 Complete Installing MATLAB Compiler Runtime 7 17 About 15 minutes remaining MathWorks Figure 4 4 MATLAB Compiler Run Time Installing 8 Once the MATLAB Compiler Run Time is finished installing select Finish 9 Installation is now complete Open HS_XX_Stim_Control exe The HS XX Stim Control Software will boot and 1s ready to be used Neuralynx_HS XX Stim Control File Connection Software Speed Help Configuration Pa

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