FasTrans Automatic Pipettor
Contents
1. General Safety Instructions for FasTrans Operation AN Shutdown in the Event of Emergency In the event of an emergency the power supply to the FasTrans and its components must be cut by pulling the power connector from the socket Make sure that the mains plug is easily accessible Caution The PC may lose data and the operating system may suffer damage from emergency shut down action Operating substances dangerous substances The operator is responsible for the selection of substances used in the process as well as for their safe handling This is particularly important for radioactive infectious poisonous corrosive combustible explosive and otherwise dangerous substances When handling dangerous substances local safety instructions and guidelines must be observed Warnings on the labels must be always observed Only use clearly marked containers as well as protective goggles and rubber gloves Biological samples have to be handled according to local guidelines regarding the handling of infectious material Cleaning and decontamination measures The operator is responsible for carrying out suitable decontamination should the device be contaminated externally or internally with dangerous substances Spots drops or larger spillages should be removed and cleaned using an absorbent material such as cotton wool laboratory wiping cloths or cellulose The affected areas are then to be wiped with an Incidin Plus soluti2. 11 5 FasTrans Setup 13 6 Installation amp Start Up 15 6 1 15 6 2 Turning the FasTrans On amp Off 16 7 Operations arenile 17 7 1 Loading the FasTrans 17 7 2 Changing T IpzLrays 5 aii i eo lia ee ner tede 17 7 3 Changing the Pipettor Head sse 18 8 CCESSOT S MR 19 8 1 1 iii iei totg te ex lion aan iaia 19 8 2 Pipettor Head Types nennen nnns 19 8 3 Used Adapters and Sample Blocks 20 9 Preventive Maintenance amp Care 23 10 DIS DOS Al eee ea e Te OEE 25 11 List of Illustrations ornare eere 27 Automatic Pipettor FasTrans Version 07 2009 1 Contents 2 Version 07 2009 Automatic Pipettor FasTrans Introduction Intended Purpose 1 Introduction 1 1 Intended Purpose The FasTrans is an automatic pipettor designed for routine laboratory jobs with small to medium sample throughput in automated procedures to prepare media plates notably PCR plates You should carefully read this Manual before you start working with the FasTrans Make sure that all safety instructions are properly followed The FasTrans may only be operated by duly trained personnel 1 2 Notes Regarding Use of thi
3. manual motion of the pipettor head or the bridge Do not use force This might cause damage to the FasTrans Turning automatic pipettor on Triggering program session 1 Use the main power switch to turn the FasTrans on 2 Trigger FasTrans control software session Communication between the FasTrans and the PC will be established Select pipetting program Fill base plate pockets of automatic pipettor with plates and tubes as required by your current program layout 5 Launch pipetting program refer to Trigger Program section of software user manual Note On failure to observe the prescribed sequence of powering on actions the software will initially start in demo mode You may then establish communi cation between the FasTrans and the PC by selecting the Options Settings menu command and unmarking the Demo check box in the Options dialog screen open Turning automatic pipettor off On completion of work with the automatic pipettor all plates and tubes must be removed from their retainer pockets There is no prescribed order of actions for turning power to the PC and the automatic pipettor off Version 07 2009 Automatic Pipettor FasTrans Operation Loading the FasTrans 7 Operation FasTrans operation runs under the control of an identically named software package FasTrans 7 1 Loading the FasTrans Caution Make sure that all plates or adapters are correctly mounted in t
4. uL 1 channel 30 1 02 250 uL 1 channel 250 1 03 30 uL 3 channel 30 3 04 250 uL 3 channel 250 3 05 30 uL 4 channel 30 4 06 250 uL 4 channel 250 4 07 30 uL 6 channel 30 6 08 250 uL 6 channel 250 6 cr Note Remember to set the installed pipettor head in your software Automatic Pipettor FasTrans Version 07 2009 19 Accessories Used Adapters and Sample Blocks 8 3 Used Adapters and Sample Blocks MTP adapter 12 mm gt MTP adapter 30 mm Waste box TipTray adapter Centrifugal adapter pipeeting station and cooling block metal for microplate 2x36 LP Centrifugal adapter pipeeting station and cooling block metal for microplate 96 LP Block 0 2 ml for 0 2 mL tubes and strips unskirted PCR plates 96 tubes Block 0 2 ml for skirted and semiskirted PCR plates 96 tubes Block 2 0 ml for 1 5 und 2 0 mL tubes 24 tubes 20 Version 07 2009 Automatic Pipettor FasTrans Accessories Used Adapters and Sample Blocks EN Adapter for 0 2 ml MTP Block 0 5 mL Block combi for 0 2 mL 0 5 mL 1 5 2 0 mL 8 of each in one block 24 zubes Block for SpeedCycler microplate 96 LP Adapter for LightCycler Adapter for Quiagen Loading Block 96 x 0 2 mL MTP adapter Automatic Pipettor FasTrans Version 07 2009 21 Accessories Used Adapters and Sample Blocks 22 Version 07 2009 Automatic Pipettor FasTrans Preventive Maintenance amp Care 9 Preventive Ma
5. you do not moisten affixed inscription labels or lettering if you use a spray disinfectant for decontamination Adapters may be cleaned with ethanol or a disinfectant if necessary They are not autoclavable Likewise UV decontamination may be removed from the FasTrans its pipettor head or from adapters Use only suitable UV decontamination lamps for this purpose Before reshipment of a FasTrans which has been used to process infectious material to Analytik Jena AG for necessary service work it must be decontaminated and related documentary proof provided Automatic Pipettor FasTrans Version 07 2009 23 Preventive Maintenance amp Care 24 Version 07 2009 Automatic Pipettor FasTrans Disposal 10 Disposal The owner operator is required to dispose of any waste material sample material resulting from FasTrans operation in accordance with binding law and local regulations On expiry of its service life the FasTrans including electronic components must be disposed as electronic waste under the latest binding provisions of law Automatic Pipettor FasTrans Version 07 2009 25 Disposal 26 Version 07 2009 Automatic Pipettor FasTrans List of Illustrations 11 List of Illustrations Fig 1 Components of the FasTrans front 13 Fig 2 Connections on rear panel of 13 Fig 3
6. 0 1 e DIN EN 61010 2 101 EMC compatibility The FasTrans has been tested for radio interference elimination and interference immunity and fulfills the requirements stipulated by e DIN EN 61326 2 6 e DIN EN 61326 1 8 Version 07 2009 Automatic Pipettor FasTrans FasTrans Technical Data FasTrans Technical Data Number of retainer pockets 9 x MTP Standard Interfaces 1x RS 232 for communication with PC Line voltage 100 V to 240 V Power consumption 80 VA Frequency 50 60 Hz Fusing None Phys dimensions 595 mm x 495 mm x 462 mm WxDXH Weight 23 kg Temperatures Working range Transportation 15 C to 35 C 20 C to 65 Tips 20 50 200 LL no filter Tecan Genesis compatible 10 50 175 uL with filter Tecan Genesis compatible Working volume 0 5 to 30 pl or 0 5 to 200 uL respectively Automatic Pipettor FasTrans Version 07 2009 FasTrans Technical Data 10 Version 07 2009 Automatic Pipettor FasTrans Transportation amp Installation Requirements Transportation amp Storage 4 Transportation amp Installation Requirements 4 1 Transportation amp Storage A Danger The weight of the FasTrans is 23 kg Therefore the device must be transported by at least 2 persons Removing all samples sample adapter tips and trays before the transport Do not leave any dangerous substances at the transport in the device For tran
7. Adapter with tip tray 0 m 17 Fig 4 Pipettor h6ad u si Wied diene iii 18 Automatic Pipettor FasTrans Version 07 2009 27
8. Pipettor FasTrans FasTrans Setup 5 FasTrans Setup Components 1 Tiltable front view panel tilted up 2 Pipettor head 3 Base plate with 9 positions 4 Status light for voltage supply analytikjena Fig 1 Components of the FasTrans front view Rear connections The terminals of the FasTrans are located on its rear panel The FasTrans must be linked up with the COM port of the PC Nameplate Main power switch Power inlet Terminal for PC COM interface A ON Fig 2 Connections on rear panel of FasTrans Automatic Pipettor FasTrans Version 07 2009 13 FasTrans Setup 14 Version 07 2009 Automatic Pipettor FasTrans Installation amp Start Up Installation 6 Installation amp Start Up 6 1 Installation A Watch Pipettor Position The FasTrans may only be transported in an upright position use markers on shipping case for guidance Do not allow the FasTrans to topple over whilst unpacking Electrical Shock Check that the pipettor s nameplate specifications for operating voltage match your actual power supply values Operation at any voltage other than the specified operating voltage level may result in destruction of the pipettor Place the FasTrans onto a desktop base with adequate spacing from other systems A minimum clearance of 10 cm must be provided around the FasTrans Use the COM interface port for FasTrans connection to the PC Us
9. analytikjena FasTrans Automatic Pipettor 4 s Analytik Jena AG analytikjena Kundendienst Konrad Zuse Str 1 07745 Jena Phone Hotline 4 49 3641 77 7407 Fax 49 3641 77 7449 e mail service analytik jena de General information about Analytik Jena AG on the internet http www analytik jena de Copyrights and Trademarks Microsoft Windows 2000 Windows XP Windows NT MS Excel are registered trademarks of Microsoft Corp Publication No 9331 006 23 Edition July 2009 Technical documentation made by Analytik Jena AG This documentation describes the state of this product at the time of publishing It need not necessarily agree with future versions of the product Subject to change Copyright 2009 Analytik Jena AG Contents Contents 1 Introduction 3 1 1 Intended Purpose nii e timen asi 3 1 2 Notes Regarding Use of this 3 2 Safety Instructions ee eine einen 5 2 1 Warning Labels on the FasTrans i 5 2 2 General Safety Instructions for FasTrans Operation 5 2 3 Standards amp Guidelines 8 3 FasTrans Technical Data 9 4 Transportation amp Installation Requirements 11 4 1 Transportation amp Storage i 11 4 2 Installation Requirements
10. e the power cord enclosed to connect the FasTrans to power supply Tum power supply on using the FasTrans main power switch Start the FasTrans program software Select Process Initialize pipettor menu command Once communication between the PC and the FasTrans has been successfully established the pipettor head will move into the rear far right corner of the FasTrans If the pipettor head already was in this position prior to initialization homing motion may be almost unperceivable in some cases In the event of failure to establish FasTrans PC communication you should check for correct interface settings e Select Options Interface menu command in FasTrans program e Setthe connected PC interface port once the dialog screen has opened e Select Options Settings menu command e Disable the Demo check box the Options dialog screen then click on OK e Repeat the test procedure for pipettor initialization On successful completion thereof the FasTrans is operational Automatic Pipettor FasTrans Version 07 2009 15 Installation amp Start Up Turning the FasTrans On amp Off 6 2 Turning the FasTrans On amp Off Never trigger a motion sequence of the pipettor head unless you have placed the appropriate storage container tip tray with tips into the specified retainer pocket Failure to comply might cause damage to the FasTrans Refrain from any kind of unsolicited not prompted by software
11. e there is danger of explosion Water Take adequate precautions to prevent water from penetrating into the inner FasTrans space The pipettor might suffer damage Danger of Corrosion Do not install the FasTrans in the direct vicinity of areas where aggressive vapours are present for example strongly etching acidic or caustic fumes Fumes of this type may corrode terminals or mechanical parts of the pipettor Do not place objects or containers with liquid onto the FasTrans for storage Close the front view panel before triggering any kind of FasTrans action While a pipetting job is being performed or during other required motion of the pipettor head the tiltable front panel must always be closed tilted down This is necessary to ensure the safety of personnel and the proper and precise function of the FasTrans gt gt b DB Keep clear of mechanically moving parts Do not place your hand or fingers into the working range of the pipettor head once you have triggered a motion sequence via the software command Automatic Pipettor FasTrans Version 07 2009 7 Safety Instructions Standards amp Guidelines 2 3 Standards amp Guidelines The FasTrans has been designed built and tested to comply with the following standards and guidelines Safety class and safety type The FasTrans belongs to safety class II The casing has safety type IP 20 Device safety The FasTrans conforms to the safety standards e DIN EN 6101
12. he adapter 4 Exert vertical pressure from above until you hear the locking pads on either side snap into locked position 7 3 Changing the Pipettor Head The FasTrans is equipped with replaceable pipettor heads pF gt E 1 Locking lever 5 Upper guide cone 2 Bridge 6 Lower guide cone 3 Pipettor head 4 Tipholder Fig 4 Pipettor head 1 Select Process Head Change software command for conversion to other pipettor head The bridge will move to the front 2 Pushlocking lever up with one hand At the same time remove pipettor head from the front with the other hand Place new pipettor head onto the lower guide cones Push pipettor head backwards until the locking lever snaps into place 18 Version 07 2009 Automatic Pipettor FasTrans Accessories Tip Types 8 Accessories 8 1 Tip Types Use only recommended types of tips The FasTrans only accepts Tecan Genesis compatible tips The following tip types may be used for operation Volume Material Type 20 uL Pure polypropylene sterile non sterile 10 uL Pure polypropylene ART filter 50 uL Pure polypropylene sterile non sterile 50 uL Pure polypropylene ART filter 200 uL Pure polypropylene sterile non sterile 175 uL Pure polypropylene ART filter 8 2 Pipettor Head Types Different types of pipettor heads are available for FasTrans operation Type Volume Channel s 01 30
13. heir respective positions Any kind of misplacement may result in damage to the FasTrans or to plates The FasTrans provides nine pockets for the mounting of plates or adapters in standard micro plate format tip trays and dump boxes onto its base plate To insert a selected plate with adapters or tubes into a given retainer pocket proceed as follows e Install adapters and tubes only in the pocket positions which are specified by your software program e Insert an adapter with tip tray so that the guide pin 4 Fig 3 is located on the left hand side e Place the dump box as centrally as possible so used tips need not be unnecessarily handled over plates e Plates which underwent software calibration together with an adapter must be installed with this adapter Note variances in height 7 2 Changing Tip Trays Adapter Locking pad Tip tray Ron Guide pin Fig 3 Adapter with tip tray Automatic Pipettor FasTrans Version 07 2009 17 Operation Changing the Pipettor Head A tip tray must be inserted into and firmly clamped in an adapter for tip trays 1 To retrieve a tip tray 3 from an adapter 1 press both thumbs on one of the two long sides against the tray from below until the tray is mechanically unlocked 2 Then release the tray on the other long side in the same manner Having done this place a new tray onto the adapter Make sure that the tray s groove is located around the guide pin of t
14. intenance amp Care The FasTrans is largely maintenance free Required maintenance and care actions are limited to the cleaning of shielding and cover panel surfaces and of the base plate Use only spare parts from Analytik Jena AG Consumable materials and wear amp tear parts can be ordered from our Customer Service department Cleaning 1 Use absorbent material to immediately wipe shielding cover panels or the desktop surface clean of pockets of spilt liquid Follow safety instructions issued by the manufacturers of such liquids when doing this 2 Usea soft clean piece of cloth which may be slightly moistened with a commercially available neutral cleaning agent to wipe away contamination from the pipettor Cleaning in medical laboratory Where the FasTrans is required to process infectious material special care must be taken because the FasTrans cannot be decontaminated as a whole unit Contamination as a consequence of normal operation is limited to the base plate Clearly visible pockets of contamination must be promptly removed using adequate means and substances Care must be taken to prevent solvent from penetrating into the inner pipettor space during cleaning procedures For cleaning recommended disinfectants are Decosept AF spray disinfectant from Dr Schuhmacher GmbH Meliseptol HBV wipes from B Braun For decontamination wipe the FasTrans and the adapter surfaces down with a non shedding piece of cloth Make sure
15. on Before another cleaning or decontamination procedure is used as that prescribed by the manufacturer the user shall check with the manufacturer that the intended procedure will not damage the device Danger The weight of the FasTrans is 23 kg Therefore the device must be transported by at least 2 persons Removing all samples sample adapter tips and trays before the transport Do not leave any dangerous substances at the transport in the device Electrical Shock The FasTrans requires an electric power supply for operation There is life threatening electric voltage present at several points inside the system The power connector may only be plugged into a socket with PE contact in order to maintain class 1 protection level protective conductor terminal of the pipettor This protective function must not be rendered ineffective by an extension without PE capability Version 07 2009 Automatic Pipettor FasTrans Safety Instructions General Safety Instructions for FasTrans Operation Remember to turn power off and pull the power connector before you open the FasTrans or remove any of its cover panels Check that the nameplate specification for operating voltage on the pipettor s back wall complies with the line voltage level that is actually provided by the selected power socket Operation at any voltage other than the specified operating voltage may result in destruction of the pipettor No operation in rooms wher
16. r own safety and to ensure failsafe operation you should carefully read this chapter before you proceed to any kind of start up action Comply with all safety rules that you find described in this Manual as well as related control software messages and prompts displayed on the screen Intended Use The FasTrans with original accessory items may not be operated in any other way than described in this User Manual No claims for liability will be accepted by the manufacturer in the event of non conforming application of any kind including non complying use of individual assemblies or components The same shall apply to service or repair work performed by anyone other than duly authorized service personnel Claims for warranty and guarantee will be null and void in such cases Local Regulations You are required to observe local safety practices as may be appropriate for and applicable to pipettor operation e g job safety regulations accident prevention rules personal injury protection schedules Notes in this Manual providing a reference to potentially dangerous situations cannot be regarded as replacing compulsory industrial labour protection laws Personnel The FasTrans may not be operated by anyone other than fully trained and qualified personnel Knowledge of this Manual is a vital prerequisite and assumed to have been acquired before pipettor operation Automatic Pipettor FasTrans Version 07 2009 5 Safety Instructions
17. s Manual The following warning and information signs are used throughout this Manual Danger A warning of this kind must be followed in all circumstances This is necessary to prevent physical injury or harm to people Caution Instructions of this kind must be followed in order to prevent damage to the pipettor Risk of electric shock gt gt Note Provides useful advice that should not be disregarded if correct results are to be obtained For the purpose of this Manual the following rules will apply e Chapters and illustrations are consecutively numbered e Each illustration has its own image caption e Specified process steps are numbered e Cross references to other Manual sections are marked with an arrow example Introduction on page 3 Automatic Pipettor FasTrans Version 07 2009 3 Introduction Notes Regarding Use of this Manual 4 Version 07 2009 Automatic Pipettor FasTrans Safety Instructions Warning Labels on the FasTrans 2 2 1 2 2 Safety Instructions Warning Labels on the FasTrans Follow all warning labels on the FasTrans A special warning label is affixed to the baseplate of the FasTrans A Hand Injury Warning Do not place your hand or fingers into the inner FasTrans space while a pipetting sequence is in progress You might suffer physical injury from mechanically moving parts General Safety Instructions for FasTrans Operation AN For you
18. sportation and storage the following ambient conditions must be observed e Temperature range 40 70 C e Rel air humidity up to 95 4 2 Installation Requirements Caution The following rules must always be observed for Installation Do not place any objects onto the FasTrans for storage Do not operate the pipettor in rooms with an explosive atmosphere Make sure there are no aggressive vapours for example strongly etching acidic or caustic fumes in the direct surroundings of the pipettor The installation site must meet the following requirements e FasTrans operating site must be free from drafts dust etching vapours and vibration FasTrans installation in the vicinity of electromagnetic fields e g motors e Prevent the presence of drops of water splashes and spray near the FasTrans Donot expose the FasTrans to direct sunlight or radiator emissions e Operating temperature range 15 C to 4 35 C e Rel air humidity for operation up to 90 at 30 Footprint requirements With physical dimensions as specified above and an additional clearance of approximately 100 mm on all sides the FasTrans requires a minimum footprint of 800 x 700 mm approx plus a base areas for the PC and the display Screen Automatic Pipettor FasTrans Version 07 2009 11 Transportation amp Installation Requirements Installation Requirements 12 Version 07 2009 Automatic
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