Bitterness System Method
Contents
1. in four days with different environmental conditions Analyzer 1 Analyzer 2 Analyzer 3 Zin 22 26 24 x Average Ths 7 6 7 1 sD 0 41 0 29 0 23 2 CV 5 66 3 81 3 33 Max Min 1 4 1 1 0 9 rin 19 24 25 x Average 7 4 7 2 7 1 8 SD 0 36 0 35 0 23 2 CV 4 91 4 78 3 28 Max Min 1 3 1 8 1 0 rin 2m 28 27 3 Average 7 5 7 1 TA SD 0 32 0 31 0 24 o cv 4 24 4 38 3 25 Max Min 1 5 1 2 0 9 in 28 26 25 2 Average 7 4 7 7 7 6 SD 0 31 0 42 0 27 o cv 4 19 5 52 3 50 Max Min 1 3 1 5 1 1 OTHER REMARKS The results obtained in individual laboratories may differ from the given performance data due to e g sample matrix concentrations or analysis environment Each laboratory is responsible to verify the method to prove the analysis performance WASTE MANAGEMENT Please refer to local legal requirements It is recommended to empty the analyzer cuvette waste bin and waste water daily Emptying should be done immediately after the analysis when using hazardous reagents solutions Note If using reagents solutions that react with each other cuvette waste bin and waste water should be emptied and washed between use of these reagents BIBLIOGRAPHY REFERENCE METHODS 1 EBC 9 8 Bitterness in beer 2 EBC 8 8 Bitter of wort 3 ASBC Beer 23 Beer Bitterness 4 MEBAK 2 18 1 ADDITIONAL MATERIAL Certific2. Page 1 D10902_03_Insert_Bitterness_System EN Bitterness system method REF 984353 984354 984355 984353 BC System Liquid 1 x 1 liter 984354 BC Diluent 4 x 9 ml 984355 BC Eluent 20 x 15 ml INTENDED USE Reagents for photometric determination of bittering compounds in beer and wort samples using automated Thermo Scientific Gallery Plus Beermaster analyzer BC System Liquid BC Diluent and BC Eluent solutions are intended for automated Thermo Scientific Gallery Plus Beermaster analyzer for system bitterness measurement using the bitterness column BC METHOD Column extraction of bittering components from beer and wort and subsequent photometric determination Method is performed at 37 C using 275 nm filter PRINCIPLE OF THE PROCEDURE Bittering compounds are extracted from beer or wort and measured at 275 nm REAGENT INFORMATION 984353 BC System Liquid 1 x 1 liter 984354 BC Diluent 4 x 9 ml 984355 BC Eluent 20 x 15 ml Reagents should be ordered separately Typically 340 360 tests can be performed with one reagent package Solutions 984353 BC System Liquid Low pH solution for conditioning 984354 BC Diluent Sample acidification solution 984355 BC Eluent Special sample elution solution Preparation The reagents are ready to use Note After removing the cap from the 20 ml vials check that there are no bubbles on the surface of the reagent when you insert vials int
3. ate of analysis and SDS are available at www e labeling eu TSF Applications for Gallery automated analyzer are available upon request from the local sales representative Information in the Application note can change without prior notice MANUFACTURER Thermo Fisher Scientific Oy Ratastie 2 P O Box 100 Fl 01621 Vantaa Finland Tel 358 10 329200 CONTACT INFORMATION www thermoscientific com e mail system support fi thermofisher com Date of revision 2015 05 22 Changes from previous version Warnings and precautions updated General updates Thermo ClrENTIFIC Template D08896_01 2015 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries This information is not intended to encourage use of these products in any manner that might infringe the intellectual property rights of others Page 3 D10902_03_Insert_Bitterness_System Appendix Correlation to a reference method by creating a calibrated method Calibration to a reference method In this process the Gallery bitterness method is calibrated to correlate to a reference method This is achieved by using the reference method analyzed sample and its reference method assigned value as the calibrator In this calibrated method results are reported as BU Bitterness unit Correlation of Gallery bitterness method to reference method is matrix specific This means that different beer
4. compounds as such Results are given as Response values AU For this method application with calibration setting none and factor set as 1 will be used This method is suitable for e g laboratory internal bitterness value measurements where no correlation to the reference method is needed This method is preinstalled in the analyzer software Bitter AU Correlation to a reference method by creating a new calibrated method If correlation to the reference method is needed and the results should be reported as units in the reference method e g BU BU for Bitterness Unit see the Appendix for the instructions See also the Bibliography section for the list of the reference methods Quality Control QC To verify the column condition and system reagents a reference sample material must be run daily as a QC sample The control intervals and limits must be adapted to the individual laboratory requirements The results of the quality control sample s should fall within the limits pre set by the laboratory CALCULATION OF RESULTS Factor calibrated results are calculated automatically by the analyzer using a factor in use LIMITATIONS OF THE PROCEDURE Interference Possible matrix interference is avoided by groupping same type of samples to one application Due to the variability of the sample types beer wort different raw materials interference from the sample matrix should be tested by e g sample dilutions during the meth
5. ette to the sample cup from the bottom of the flask e Avoid use of plastics e Never use a filter paper for the sample preparation step Note Degassing or antifoam agents e g octanol must not be used Use of degassing agents leads to fast degradation of the column performance TEST PROCEDURE See the Bitter AU application in the Gallery Plus Beermaster analyzer or aseparate application note In this application AU stands for result unit Absorbance Unit This application contains two parts pretreatment Pretreatment definition Bitter AU and measurement Test definition Bitter AU Note The Bitter AU application measurement parameters must not be changed The sample volume in the bitterness pretreatment parameters can be changed to adjust the response level according to the sample level The changes to the parameters cannot be made in the default application but_a copy of the application must be created All new applications and changes to the pretreatment parameters must be validated by the user Materials required but not provided Deionized water aseptic and free of heavy metals and general laboratory equipment Consumables Sample Prep block ordering code 986366 56 blocks for 1680 samples Decacell cuvettes ordering code 986540 CALIBRATION AND QUALITY CONTROL Factor calibrated absolute method Gallery Plus Bitterness system can be used as a novel bitterness method measuring at 275 nm extracted bitterness
6. o the analyzer Note See the Installation manual for the instructions for how to change the BC System Liquid transportation cap to the cap with tubing installed This cap is provided with the analyzer Storage and Stability Reagents in unopened vials are stable at 15 25 C until the expiry date printed on the label Store the reagents according to the local legal requirements Refer to the Application Notes of your analyzer for the on board stability of the reagents WARNINGS AND PRECAUTIONS Bitterness system liquids are hazardous See separate sheet inside the kit for Hazardous and Precautions phrases BC System Liquid and BC Diluent H290 H315 H319 P280 P302 P352 P305 P351 P338 BC Eluent H226 H319 H336 P210 P261 P280 P305 P351 P338 Exercise the normal precautions required for handling all laboratory reagents SAMPLES Sample Type Beer wort and process samples Sample bitterness concentration All sample and method related details are in a separate application note Typical sample concentration is 5 100 BU Sample preparation e Turbid samples and samples containing precipitate must be clarified by centrifugation before analysis e Beer samples containing carbon dioxide and foam must be degassed by ultrasonication or magnetic stirrer Loss of foam must be avoided and the foam must be allowed to collapse back into the beer before taking the sample to analyzer Beer sample is recommended to pip
7. od verification in the laboratory Thermo ClrENTIFIC Template D08896_01 2015 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries This information is not intended to encourage use of these products in any manner that might infringe the intellectual property rights of others Page 2 D10902_03_Insert_Bitterness_System MEASURING RANGE The test has been developed to determine Bitterness concentrations within a measuring range from 5 to 100 BU PERFORMANCE CHARACTERISTICS The results obtained in individual laboratories may differ from the performance data given Picture below shows an example of method linearity Linearity 5 100 BU y 0 ae 0 0152 0 9953 0 20 0 18 0 16 0 14 0 12 0 10 0 08 0 06 0 04 0 02 0 00 0 20 40 60 80 100 120 Theoretical BU In this example sample used was 100 BU pale lager beer and its water dilutions In the example 200 ul pretreated sample volume and 300 ul eluent volume was used Measured Abs Determination limit Test limit low The determination limit is the lowest concentration that can be measured quantitatively The determination limit for this method is 5 BU For this determination 300 ul sample volume and 300 ul elution volume has been used Repeatibility and reproducibility Repeatibility and reproducibility study was performed with three Gallery Plus Beermaster analyzers
8. s might need separate matrix specific calibrations This is true at least in between dark and pale beer types Creating calibrated bitterness applications to the Gallery Beermaster software Copy the preset Bitter AU test definition parameters to a new test definition Rename the new test definition as e g Bitter BU1 Define the calibrators and assign the reference values in the calibrator definition window Change the calibration type from none to linear in the new test definition The calibrators should be analyzed as duplicates Additional instructions for choosing the calibrators As calibrators several different beers with different bitterness values can be used or one beer with high bitterness value and a its water dilutions Calibrators should cover the entire measuring range The values of the calibrators are measured with the reference method The value of the dilution can be calculated Calibration interval After setting up the methods the daily calibration is not typically needed To verify the calibration and column condition calibrator material or other reference sample material must be run daily as a QC sample Calibration interval must be re validated by the laboratory using the QC sample Quality Control QC Use quality control samples at least once a day and after each calibration The control intervals and limits must be adapted to the individual laboratory requirements The results of the quality con
9. trol sample s should fall within the limits pre set by the laboratory CALCULATION OF RESULTS The results are calculated automatically by the analyzer using a calibration curve or a factor Calibration Curve example 0 12 0 11 Response AU 2 o o o p i lo oO OD N O fo 0 05 15 20 25 30 35 40 Reference value BU Calibrator Response A ae Undiluted beer 0 111 38 Undiluted beer 0 113 38 Diluted beer 1 1 0 066 19 Diluted beer 1 1 0 065 19 Calibration factor of this example is 410 The sample volume in the calibration was 100 ul Note that the calibration curve is calibrator material dependent Thermo ClrENTIFIC Template D08896_01 2015 Thermo Fisher Scientific Inc All rights reserved All trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries This information is not intended to encourage use of these products in any manner that might infringe the intellectual property rights of others
Download Pdf Manuals
Related Search