Chromaflow Packing stations - GE Healthcare Life Sciences
Contents
1. Oo COLUMN zaa oorr Nc more Y f Pan PODC PIP ra F emt ens Te o L_ fe ae amp ennedo fas Fig 2 1 Connecting the Chromaflow Packing station prior to operation 1 Connect Outlet SIT to column SIT 2 Connect Outlet SIB to column SIB 3 Connect Inlet SOB to column SOB Make the following connections to join the tanks to the Chromaflow Packing station 1 Connect Inlet C to Slurry tank 2 Connect Inlet B to Water Buffer tank 3 Connect Outlet D to Waste Collection tank Note Use closed vessels to prohibit evaporation On the column mount a 3 way valve on both MPT and MPB The valves should have the same inner diameter as the column inlet outlet To be able to determine when the packing is completed connect a pressure gauge maximum range 0 to 6 bar to the mobile phase top MPT when using downward packing and to the mobile phase bottom MPB when packing with upward flow Connect a tube from the MPB valve to a drain WARNING Ensure that any tubing on the floor is placed so that the risk for tripping accidents is minimized Consult your column Instructions for Use or User manual for further instructions on connecting the column to a packing station Instructions for Use 56 3215 58 Edition AB Instructions for Use 56 3215 58 Edition AB 2 Before operation 2 4 Preoperational checks Prior to operation check that e the indicators on2. WARNING Do not exceed the rated pressure of the incoming air supply otherwise there is a risk of personal injury and damage to the column A suitable safety device must be fitted 1 6 Technical specifications 1 6 1 General The primary technical specifications for Chromaflow Packing stations are listed in the tables below Table 1 1 Dimensions and design specifications for Chromaflow Packing stations Description Footprint 810 x 715mm Height 1175 mm Weight Pack 50 115 kg Pack 100 130 kg Operating temperature 4 to 30 C Supply air pressure 6 to 7 barg Noise level maximum 85 dB A Air consumption Pack 50 500 Ni minute Pack 100 1000 NI minute Max outlet pressure 7 barg Pump capacity Pack 50 50 l min Pack 100 100 l min Instructions for Use 56 3215 58 Edition AB Instructions for Use 56 3215 58 Edition AB 1 Chromaflow Packing station Table 1 2 Materials of construction and media contact information for Chromaflow Packing stations Part Material In contact with pumped media Valve bodies 1 4435 316L forged Yes Valve diaphragms EPDM Yes Pump housings HDPE Yes Pump diaphragms PTFE coated Yes Pump balls PTFE Yes Pump inlet outlet 1 4435 316L Yes Pump seals EPDM Yes Piping 1 4435 316L Yes Frame 1 4401 316 No Wheels Polyurethane coated No Table 1 3 Interface connection dimensions and sizes for the Chr
3. 3215 58 Edition AB 3 Operation Instructions for Use 56 3215 58 Edition AB 3 Operation Prior to operating the Packing station be sure to follow the instructions laid out in Chapter 2 of this Instructions for Use IMPORTANT As stated elsewhere in this manual the packing and unpacking instructions are a general method that may not be useful for all situations Modifications to these instructions and to the connections between the packing station the column and the vessels may be necessary under other operating scenarios Contact your GE Healthcare representative for further information IMPORTANT Several of the steps in the following sections refer to the use of a pressure gauge These particular steps are part of a general method suggested by GE Healthcare although modifications can be made to these instructions that do not need a pressure gauge To order a pressure gauge please contact your GE Healthcare representative 3 1 Column packing with the Chromaflow Packing station Chromaflow columns can be packed via the bottom or top nozzle The instructions below are for packing from the top To pack from the bottom carry out the same procedure for the connections and flow path via the bottom nozzle 3 1 1 Packing from the top Note Make sure that the column has been prepared for packing as described in Chapter 3 of the Chromaflow columns Instructions for Use 1 Connect a flexible pipe hose to MPB and se
4. Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 85 65 62751830 Fax 85 65 62751829 e Australasia Tel 61 2 8820 8299 Fax 61 2 8820 8200 Austria Tel 01 57606 1613 Fax 01 57606 1614 Belgium Tel 0800 73 890 Fax 02 416 8206 Canada Tel 1 800 463 5800 Fax 1 800 567 1008 Central amp East Europe Tel 43 1972 720 Fax 43 1972 722 750 e Denmark Tel 45 70 25 24 50 Fax 45 45 16 2424 e Eire Tel 1 800 709992 Fax 44 1494 542010 e Finland amp Baltics Tel 358 9 512 3940 Fax 358 9 512 39439 e France Tel 01 69 35 67 00 Fax 01 69 41 98 77 Germany Tel 0800 9080 711 Fax 0800 9080 712 e Greater China Tel 852 2100 6300 Fax 852 2100 6338 Italy Tel 02 26001 320 Fax 02 26001 399 e Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 e Korea Tel 82 2 6201 3700 Fax 82 2 6201 3803 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 Middle East amp Africa Tel 30 210 96 00 687 Fax 30 210 96 00 693 Netherlands Tel 0800 82 82 82 1 Fax 0800 82 82 82 4 e Norway Tel 47 815 65 777 Fax 47 815 65 666 e Portugal Tel 21 417 7035 Fax 21 417 3184 e Russia CIS amp NIS Tel 7 495 956 5177 Fax 7 495 956 5176 Spain T
5. declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Note The Declaration of conformity is valid only for systems that are marked with the CE logo Contents Contents 1 Chromaflow Packing Station ccesesesessseseeeseeeeeeeees 5 1 1 Application 1 2 Approved operators L3 Product MarKINOS ss ecsccesdscseistedszcctscastsigsheesteaddenspateieseskeassactsigslecsteasiocipasencsbe 5 1 4 Safety COMPLIANCE eerte E 6 1 4 1 Use in potentially explosive atmospheres ssssssssssssssssssssseccsceseseeeeees 7 1S Sateet MO FEC OUT OFS rrr n e ERORE 8 16 Technical SPECIE OHONS erreren REEE 8 WOM General an a a ORO 8 6 27 NolS leve l innnan aeRO 9 1 6 3 Chemical resistance irs caicacacnticnataninaisamasnnnieinsianaatadninie 9 ide SLOP OG Ceo esses AEE OA IE 11 Le Operating tanes an RREN A ARAA 11 2 Before OPELATION sicsssscasasisssisssassivcvincsbatcraederesasetesosouosescase 13 2 1 UNPACKING ON delivery sesi niiina EN RR 13 2 2 Preparing the column and Packing Station s sssssss119111911101110110 13 22 1 Physical INStAlAtiON sinnis 13 22 2 Ai SUpplY serna n A a Aa 13 2 3 Connecting the Chromaflow Packing statio
6. material between the forks of the fork lift truck and the station frame 2 2 Preparing the column and Packing station 2 2 1 Physical installation Refer to the Documentation Binder for pneumatic connections Check to verify that the column has been properly leveled 2 2 2 Air supply During operation the Chromaflow Packing station requires a supply of compressed air 6 7 bar g 2 3 Connecting the Chromaflow Packing station to the column IMPORTANT The suggested connection schemes are of a general nature Users may wish to alter the configuration depending on specific needs If there is any question about the safety of the intended setup please contact your GE Healthcare representative for clarification Follow the instructions below for proper connection of the Chromaflow Packing station to the column and vessels Refer to Figure 2 1 below to clarify this operation Note Check the inner diameter of the tubing connected to the Chromaflow Packing station and the column If the inner diameter is too small it can lead to high back pressure and reduced flow capacity To avoid creating steps in the flow pathways always use the same diameter connector seals In addition to ensure a smooth transition from one diameter to another always use conical reducers Instructions for Use 56 3215 58 Edition AB 13 2 Before operation 2 3 Connecting the Chromaflow Packing station to the column 14 pan N A
7. the control panel are functioning correctly otherwise there is a risk that the Chromaflow Packing station will try to operate a column with its outlet valves closed e tubing from the Chromaflow Packing station to the inlet and outlet tanks and from the Chromaflow Packing station to the column is properly connected in accordance with the respective Instructions e all tubing is in good condition and verify that there is no risk for leakage at any connection e the outlet tubing or piping from the Chromaflow Packing station is connected to the equipment that is part of the closed system for example a tank with lid e the pneumatic air supply that furnishes the valves and pumps with compressed air is assembled correctly e at least one outlet valve on the column is open before starting the pump otherwise pressure may exceed the design limit 2 5 Priming the column and packing station Before proceeding with packing the Chromaflow Packing station and column must be primed IMPORTANT Various methods are available for priming the Chromaflow Packing station and associated column The instructions provided below are a general method that is suitable for many situations However modifications to this method may be necessary depending on the set up of the system and operating procedures If further instructions on priming or packing are needed please contact your local GE Healthcare representative UNPACKING PUMP
8. type of maintenance work while the packing station is connected to an air supply IMPORTANT Only approved operators are allowed to do maintenance work on Chromaflow Packing station The Chromaflow Packing station is designed to require a minimum of service and maintenance The only maintenance that can be safely carried out by an operator is replacement of the gaskets between the tubing and outlets of Chromaflow Packing station For ordering information refer to the Spare Parts List For maintenance work other than that specified above please contact your local GE Healthcare representative 4 2 Cleaning before maintenance service IMPORTANT Make sure that the packing station has been thoroughly cleaned to remove any infectious or aggressive fluids A Health And Safety Declaration Liability form must always be completed prior to maintenance service by GE Healthcare personnel or if the equipment is to be returned A decontamination report is enclosed in the Documentation Binder supplied with the Chromaflow Packing station 4 3 Troubleshooting e If the packing station does not function at start up i e the valves are not working check that the compressed air supply is sufficient e f the flow is not working as planned check the status of the inlet and outlet valves and that the flowpath is open 23 Index Index 24 A 6 cs 0 o 6 0 Reauneeap ne eee ror n rece een ee et 13 CLIO OOS resis da
9. 3215 58 Edition AB Index O operating panels P packing pump knob packing the column pressure gauge priming R reset button S safety compliance storage T top nozzle toggle switch trouble shooting U unpacking on delivery unpacking pump knob unpacking the column 25 Index 26 Instructions for Use 56 3215 58 Edition AB www gehealthcare com protein purification bioprocess GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden Chromaflow and Drop Design are trademarks of GE Healthcare companies GE imagination at work and GE monogram are trademarks of General Electric Company Triton is a trademark of Union Carbide Chemicals Tween is a trademark of ICI Americas Inc GE Healthcare Bio Sciences AB a General Electric company All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information Chromaflow is covered by the following patents US 5 213 683 US 5 282 973 GB 2 307 028B EP 776243 2006 General Electric Company All rights reserved GE Healthcare Bio Sciences AB Bjorkgatan 30 751 84 Uppsala
10. CLEC wyj ececeoevecocecos EXTENT OF PACKING STATION yy v Fig 2 2 Schematic flow chart when priming 15 2 Before operation 2 5 Priming the column and packing station 16 To prime the Chromaflow Packing station and column refer to the schematic above and follow these steps 1 9 Set the top nozzle to the UNPACK position and the bottom nozzle to the RUN position see left Connect a fluid source to MPB and slowly run liquid into MPB This will remove air from the MPB and from beneath the bed support Allow sufficient time to ensure that the bottom distributor is flooded Set the bottom nozzle to the PACK position Open B and SIB and start the packing pump to fill the vessel via the bottom nozzle until liquid exits through SOT to the collection tank then stop the packing pump Retract the top nozzle to the RUN position Continue the flow of liquid out through MPB in order to remove air bubbles from the bottom distributor Open the valve on the MPT and continue flowing liquid through SIT until all air has been driven through the top bed support and out through MPT Close MPT and allow pressure to build up without exceeding the maximum pressure Open MPT to purge the last air bubbles then stop the flow and close MPT Repeat the same procedure for MPB Retract the bottom nozzle to RUN position The Chromaflow Packing station and column are now primed and ready to pack Instructions for Use 56
11. GE Healthcare Chromaflow Packing stations Instructions for Use Chromaflow Important user information All users must read this entire Instruction for Use to fully understand the safe use of the Chromaflow Packing station Safety notices This Instruction for Use contains safety notices concerning the correct use of the column This information is provided to allow users to avoid personal injury and or damage of the equipment and to present information that can optimize the use of the columns The various types of safety notices include WARNING The WARNING sign and box highlights instructions that must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION The CAUTION box highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood IMPORTANT The IMPORTANT box highlights instructions or details relating to instructions that will help to ensure optimal use of the equipment Note The Note is used to indicate information important for trouble free and optimal use of the product Declaration of conformity This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding
12. ING STATION COLUM Fig 3 4 Schematic flow diagram for cleaning the column and Packing station Fill the Packing Buffer tank with cleaning solution Then select the following process positions 1 With both top and bottom nozzles in the UNPACK position fully inserted into the column configure the packing station in the following manner Inlet B cleaning solution Outlet A to collection vessel waste Outlet D to collection vessel waste Turn the packing pump on and toggle between the column SIT and SIB Cleaning solution will spray out from the top and bottom nozzles respectively Continue this procedure for some minutes Stop the packing pump and run the unpacking pump until all cleaning solution is removed from the column Retract the top nozzles to its RUN position level with the bed support Run both pumps simultaneously Stop the pumps when the top bed support appears to be free of beads If there still are cleaning liquid in the column remove it by inserting the top nozzle to its unpack position and use the unpacking pump to empty the column To clean the mobile phases select the PACK position on the bottom nozzle mid position Select the UNPACK position on the top nozzle fully inserted Pump liquid via the bottom nozzle Once the column is full liquid starts to exit via SOT Check the column pressure on the packing pressure gauge It should not rise above the column pressure r
13. ating lower pressure by reducing the pump speed 21 3 Operation 3 4 After use 9 Open MPB and close SOT by retracting the top nozzle when column interior appears to be free of air Liquid will now leave via MPB and clean the bottom mobile phase 10 To clean the top mobile phase close MPB and open MPT 11 Stop the pump and close MPB and MPT when both mobile phases are cleaned Insert top and bottom nozzle to unpack position and use the unpacking pump to empty the column 12 Finally repeat steps 1 to 9 using 20 ethanol then allow the column to drain The column is now clean and ready to pack or store 3 4 After use Rinse the Chromaflow Packing station carefully after it has been used and make sure there is no adsorbent remaining Residual adsorbent can restrict or block the flow passages resulting in an increase in pressure 3 5 Disconnecting the Chromaflow Packing station from column CAUTION Always TURN OFF the compressed air supply before disconnecting the Chromaflow Packing station from the column Clean the Chromaflow Packing station carefully making sure that there is no hazardous liquid remaining in the tubing Remove the tubing between the Chromaflow Packing station and the column 22 Instructions for Use 56 3215 58 Edition AB 4 Maintenance and troubleshooting 4 Maintenance and troubleshooting A Instructions for Use 56 3215 58 Edition AB 4 1 General WARNING Do not perform any
14. cure a loop that is above the top of the maximum bed height This will prevent siphoning 2 With the top nozzle in the RUN position open C and SIT and start the packing pump Slurry will flow through the pump into the top nozzle and back to the slurry tank via SOT and the flexible hose thus priming the packing lines 17 3 Operation 3 1 Column packing with the Chromaflow Packing station PATONG PUMP AR PRESSURE QO Pa overte 7 j V eal PACING PUMP UP ACID PAMP CHTENT OF PACKING STATON Fig 3 1 Schematic flow diagram of the packing set up 3 Atthis time it is possible to adjust the flow to suit the optimized flow rate for the media in use Adjust the pump speed by using the FINE ADJUSTMENT knob on the operating panel and reading the resulting flow from a flow meter alternatively read the flow by using a stop watch and bucket Adjusting the flow can also be achieved by using the pressure gauge and measuring the column pressure drop Refer to media instructions for approximate flow rates 4 Open MPB and insert the top nozzle to the PACK position 5 Once the column is packed retract the top nozzle and change the suction to inlet B to flush out the packing lines with packing buffer 6 Close MPB Note Remember to stir the media slurry during packing to prevent it from settling 7 Stop the pump and disconnect suction B 8 Runthe pump dry to remove most of the residual li
15. direct outlets A and D to the collection vessel to remove slurred media 19 3 Operation 3 2 Unpacking the column with the Chromaflow Packing station 20 Phase 2 A PACING PUMP AUR PRESSURE pacana oren gt POLK PAIP Ton ENTENT OF PAGING STATION Fig 3 3 Schematic flow diagram step 2 for unpacking set up 1 Start the unpacking pump Slurry is removed from the column via outlet D and air is entered into the column via inlet A Note Ensure that the orifice leading from Collection to the column via outlet A is above the surface of the slurry to allow air to be pumped 2 Change to B and use the packing pump toggling between the top and bottom nozzles to flush out the remaining media 3 When empty stop the pumps Set the top nozzle to Pack position Pump packing buffer through SIT and simultaneously run the unpacking pump for a final rinse of the bed supports and the complete column Note You can monitor the column pressure so that it is not subjected to over pressure 4 Retract both nozzles and flush the lines and the unpacking pump with packing buffer The unpacking procedure is now completed Instructions for Use 56 3215 58 Edition AB Instructions for Use 56 3215 58 Edition AB 3 Operation 3 3 Cleaning the column Connect the column as shown in the figure below PACING AMP AR PRESSURE Pace burren tta PACEING PUMP tea eee UNPACEAG PP hon EXTENT OF PACK
16. e rating plate Chromaflow Code no Serial number Year of manufacturing Operating temperature TS Supply air pressure Airt consurnption Max outlet pressure Pump capacity I13G Amersham C e Yo Box Fig 1 1 The Chromaflow Packing station rating plate Note A label in the local language is only available in countries within the EU If not delivered with Chromaflow Packing station it can be ordered from your local GE Healthcare office or GE Healthcare Bio sciences AB in Sweden 1 4 Safety compliance The CE marked Chromaflow Packing station complies with the EU directive described in the Declaration of Conformity in this Instructions for Use The compliance with the directive is valid only under the condition that the Chromaflow Packing station is installed operated and maintained according to the Documentation Binder and Instructions for Use To maintain compliance use only spare parts approved or supplied by GE Healthcare Instructions for Use 56 3215 58 Edition AB Instructions for Use 56 3215 58 Edition AB 1 Chromaflow Packing station 1 4 1 Use in potentially explosive atmospheres EX marking The EX code on the identification label defines the level of explosion protection of the equipment The EX category of the equipment must be in conformity with the protection level of the room where it is to be used Ex ll 3G Equipment in this category is designed to be capable of functioning in conf
17. e valves and pumps are controlled pneumatically and are operated from the control panel As valves and pumps are brought into operation indicators on the control panel display the relevant flow paths Two models of Chromaflow Packing station are available Pack 50 which utilizes two pumps each with a maximum 50 l min capacity and Pack 100 which utilizes two pumps each with 100 I min capacity For drawings and other relevant documentation please refer to the appropriate section in the Documentation Binder 1 1 Application The Chromaflow Packing station is a semi automatic packing station designed for use with Chromaflow columns GE Healthcare is not liable if the Chromaflow Packing station is used with equipment other than that specified in this manual 1 2 Approved operators To use the Chromaflow Packing station the operator must have read understood and be aquainted with this Instructions for Use 1 3 Product markings The Chromaflow Packing station is marked with GE Healthcare Code No 18 1163 74 Pack 50 18 1162 08 Pack 100 e Serial number e Year of manufacture e Operating temperature 4 to 30 C e Supply air pressure 6 to 7 barg e Air consumption 500 Ni min Pack 50 1000 NI min Pack 100 e Maximum outlet pressure 7 barg 1 Chromaflow Packing station 1 4 Safety compliance e Pump capacity 50 I min Pack 50 100 I min Pack 100 e EX category Il 3G e These rating marks can be found on th
18. el 902 11 72 65 Fax 935 94 49 65 e Sweden Tel 018 612 1900 Fax 018 612 1910 e Switzerland Tel 0848 8028 10 Fax 0848 8028 11 UK Tel 0800 515 313 Fax 0800 616 927 e USA Tel 1 800 526 3593 Fax 1 877 295 8102 imagination at work Instructions for Use 56 3215 58 Edition AB 06 2006
19. idizers such as peroxides Fluorine and halenogenated compounds Chlorinated solvents such as methylene chloride Esters Aromatic hydrocarbons such as toluene High concentrations of strong acids Table 1 4 Chemical resistance of materials in Chromaflow Packing stations Substance Concentration Resistance by volume 60 90 days Acetic acid 1 7 M OK Ethanol 40 OK Ethylene glycol 50 OK Formaldehyde 1 7M OK Formic acid 10 OK Glycerol 100 OK Hydrochloric acid 0 1M See note 1 Isopropyl alcohol 30 OK Nitric acid 0 1M OK n Propanol 100 OK Phosphoric acid 25 See note 2 Sodium chloride 0 5 M See note 1 Sodium hydroxide 2M OK Trifluoroacetic acid 0 1 OK Triton X 100 100 OK Tween Tri n butyl phosphate 1 0 3 OK Urea 8M OK Note 1 Not recommended The stainless steel will be affected Note 2 Limited resistance Instructions for Use 56 3215 58 Edition AB Instructions for Use 56 3215 58 Edition AB 1 Chromaflow Packing station 1 7 Storage Prior to storage Chromaflow Packing station must be cleaned properly to ensure that all slurry is removed from the tubing The Chromaflow Packing station must be stored at room temperature and must not be exposed to extreme temperatures A common storage technique is to seal off all inlets and outlets and use 20 ethanol as storage solution However long term and other special storage conditions may require o
20. ivating the system will start the pumps at the maximum level delivering a sudden high level of noise and also an immediate high pressure increase in the system You are advised to reduce the pump setting prior to using the RESET button A N WARNING When the system is reactivated using the RESET button the pumps 3 FINE ADJUSTMENT Use this knob to make fine scale adjustments to the PACKING PUMP air pressure 4 PACKING PUMP AIR PRESSURE Controls the amount of air delivered to the PACKING PUMP Note This bypasses the FINE ADJUSTMENT knob 5 UNPACKING PUMP AIR PRESSURE Controls the amount of air delivered to the UNPACKING PUMP 6 NOZZLE CONTROL Activates or deactivates 0 the TOP and BOTTOM NOZZLE toggle switches Note The nozzle control should always be set to 0 when the nozzle control function is not used 7 TOP NOZZLE Sets the TOP NOZZLE to RUN PACK or UNPACK 8 BOTTOM NOZZLE Sets the BOTTOM NOZZLE to RUN PACK or UNPACK 12 Instructions for Use 56 3215 58 Edition AB 2 Before operation 2 Before operation 2 1 Unpacking on delivery When removing the Chromaflow Packing station from the shipping crate use a fork lift positioned under the base frame Be sure to place the packing station on a level surface CAUTION During lifting operations to help prevent damage to the surfaces of the Chromaflow Packing station and thus minimize the risk of rusting you are advised to place a soft
21. n to the column 13 24 Preoperational CHECKS wacccccsssssssssscccccssssssussseecsessssssssnesseessssssssssneeeeeeeee 15 2 5 Priming the column and packing station w eecceeeeescsecsssssssseeeeeeseen 15 3 Operati N ienee iiaii 17 3 1 Column packing with the Chromaflow Packing station 17 3 1 Ve POEKING frm tHe tOP inaa a 17 3 2 Unpacking the column with the Chromaflow Packing station 19 B S CISCMMG TIC OMA e A E ATERS 21 ZA Ate USE nienie a e e N 22 3 5 Disconnecting the Chromaflow Packing station from column 22 Instructions for Use 56 3215 58 Edition AB iii Contents 4 Maintenance and troubleshooting sssseceeeees 23 E S D AEE ME EA E E hacen rite tat icant O 23 4 2 Cleaning before maintenance service cccsessssssssssceessssseseseseenen 23 GS x TOUDICSHOOUING sro r E tecce dante nteusenennaselles 23 TIN OK AEA EAA A EERE E E tenance ORO 24 iv Instructions for Use 56 3215 58 Edition AB 1 Chromaflow Packing station 1 Chromaflow Packing station Instructions for Use 56 3215 58 Edition AB The Chromaflow Packing station is a semi automatic packing station designed for use with Chromaflow columns Using a Chromaflow Packing station with Chromaflow columns makes column preparation and packing a simple and clean operation as well as helping to reduce operator time The Chromaflow Packing station consists of a control panel mounted on a frame with two pumps and four valves housed underneath Th
22. omaflow Packing station Interface Dimension internal Flange type and diameter size Packing station inlet B Pack 50 1 22 1 mm Pack 50 TC 1 Pack 100 1 5 34 8 mm Pack 100 TC 1 5 Packing station inlet C Pack 50 1 22 1 mm Pack 50 TC 1 Pack 100 1 5 34 8 mm Pack 100 TC 1 5 Packing station inlet SOB 1 22 1 mm TC 1 Packing station outlet D 1 22 1 mm TEL Packing station outlet SIT 1 22 1 mm TC 1 Packing station outlet SIB 1 22 1 mm TC 1 1 6 2 Noise level The noise level from Chromaflow Packing station has been measured during packing unpacking The test was performed at maximum pump capacity at a point 1 m from the surface of the packing station and 1 6 m from the floor Max measured noise level 85 dB A 1 6 3 Chemical resistance Table 1 4 is a guide to the resistance of Chromaflow Packing station to chemicals and solvents commonly used in process chromatography The information has been compiled from published material from several sources not from individual tests on the components of Chromaflow Packing station 1 Chromaflow Packing station 1 6 Technical specifications 10 It should be noted that the effects of a chemical will be more severe at higher temperatures and pressures and that combined effects have not been taken into consideration In general the use of the following chemicals should be avoided Powerful ox
23. ormity with the operating parameters established by the manufacturer and ensuring a normal level of protection Equipment in this category is intended for use in areas in which explosive atmospheres caused by gases vapors or mists are likely to occur or if they do occur are likely to do so only infrequently and for a short period only When used in potentially explosive atmospheres the following precautions should always be observed e Avoid discharge from static electricity by grounding earthing the column Use a grounding wire connected from the plant grounding network to the Packing station grounding plate see Figure below A grounding kit is included with the delivery of the Chromaflow Packing station Grounding connection Fig 1 2 The grounding connection on the Chromaflow Packing station 1 Chromaflow Packing station 1 5 Safety precautions gt gt gt 1 5 Safety precautions IMPORTANT Whenever the column is used always keep the Instructions for Use for the column on hand IMPORTANT The end user must ensure that all installation maintenance operation and inspection is carried out by qualified personnel who are adequately trained and understand the operating instructions WARNING Always use protective clothing appropriate with the current application to ensure personal safety during operation WARNING Pay extra attention when working close to the packing station
24. quid The column is now ready to equilibrate and test 18 Instructions for Use 56 3215 58 Edition AB Instructions for Use 56 3215 58 Edition AB 3 Operation 3 2 Unpacking the column with the Chromaflow Packing station Unpacking the column takes about 20 25 minutes Before starting with this procedure make sure that The set up is the same as for packing with the addition of e Outlets A and D are connected to the slurry vessel Phase 1 PAQUNG AAP Alt PRESSURE raceme mron amp PACING MAP UNPACKING PUMP EXTENT OF PACKING STANON Fig 3 2 Schematic flow diagram step 1 for unpacking set up 1 Fill the packing buffer tank with about four times the column bed volume of unpacking liquid Set the suction of the packing pump to B Set both top and bottom nozzles to the UNPACK position fully extended Set the discharge of the packing pump to the bottom nozzle When the slurry is recirculating easily increase the flow to maximum nn FW DN Start the packing pump on a slow setting Monitor the column pressure to assure that it does not exceed limits 7 When the media in the column begins to form a slurry switch the suction from B to C Slurry will now be recycled into the column and back to the slurry tank 8 Toggle between top and bottom nozzles until all of the recycling media is slurry On acrylic tubes breakthrough can be observed through the column wall 9 Stop the packing pump and
25. rts lh tl ate allel alt onl Al ole 5 approved Operators iy 0iet crm eas sinned wdadennandiienuaee 5 B bottom nozzle toggle SWITCH woecssssssssssessccsssessessssesssesseeessen 12 C Ee E EE EA 6 chemical resistance veaceececcceccecsecsecsessessessecsecsessecsecscsuesscsucssessesuessesseeseenes 9 leaning dar mece 0 aa sarearena ent Ae 21 connecting to the COMM wrcsscanieatomsalaauurtnatonnieaten 13 connection GIMENSIONS n ssssssssssssssssesssssssssssssoisrssssssrirrrsssrisrirssssrrrn 9 D design Specifications Ste acres te ae ta tee eu tert 8 CISCO MINCE GG ter nsin ascorrneanreercencenpsistinceesen temvonnrced immdeiteat noun er 22 E emergency Stop button s ss sssssss1ssssssso1sssotsrotsstottorsstorssstsstotssttsstetss 12 explosive atmospheres ou eecsscssseessssesssssescsssescssseccssssecessesscsssecsessuecessecss 7 F fine adjustment knob 5 cess case ceeptei ela conus mmeanssenewmneatedenimie 12 G gro ndihg the columnis ni nina 7 M MAINTENANCE oeeeecccccececssececsesseccsesecsessesecsesseceesucsecsesecaesaesueaesueseesesenensaeeess 23 MAKINI Snare a e e N e in 5 materials of COnStruction cccscescesccessessosssssssessscssescesssessessszessecsecsess 9 N noise level ssssssssesssssssoessssssseesssssnssscesssssseocssssnssoctensnssuocsrssnssocesnsossuscssrenss 9 nozzle control SWITCH wceececceccecsecssececscssessecsessessesseseesstssesseesssesseeseeseees 12 Instructions for Use 56 3215 58 Edition AB Instructions for Use 56
26. ther solutions For additional advice on storage contact your GE Healthcare service representative 1 8 Operating panels The Chromaflow Packing station is controlled via upper and lower operating panels The upper panel provides a view of the flow scheme a gauge showing the packing pump air pressure and the ability to toggle the flow path between inlets B and C and between column SIT and SIB see Figure 1 3 gt PACKING PUMP PACKING BUFFER Ye SLURRY i 9 D COLLECTION UNPACKING PUMP Fig 1 3 Diagram of the upper operating panel 11 1 Chromaflow Packing station 1 8 Operating panels The lower operating panel is the primary point for operating the packing station The following functions are available ioe f TOP NOZZLE PACKING PUMP UNPACKING PUMP wee 3 lA lA na BOTTOM NOZZLE w 5 CEN an PRESSURE Chromaflow Amersham Packing station owe Fig 1 4 Diagram of the lower operating panel 1 Emergency STOP button Stops the system when pushed All pneumatic air iS purged inlet and outlet valves are closed and both pumps stop To reactivate it twist the button out 2 RESET After reactivating the STOP button push this button and hold down for a few seconds to return the system to the same status as before the emergency STOP will start at the same setting prior to pressing the emergency STOP button If the pump setting was on maximum then react
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