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Centrifuge 5427 R - Operating manual

1. Vessels per adapter rotor international Rotor S 24 11 AT Bucket for tubes 4 x 1 5 mL Max g force 16 049 x g 2 0 mL Max speed 12 700 rem Max load per bucket 4x 3 75g e tube and contents Vessels Vessel Adapters Adapter base Max g force Capacity Order no Tube diameter Max speed Centrifugation radius Vessel 1 5 mL 2 0 mL 24 11 mm 16 049 x g 12 700 rem 8 9 cm Centrifuge 5427 R Operating manual 3 Safety rri Z 3 1 Intended use The Centrifuge 5427 R is intended exclusively for indoor use and for separating aqueous solutions and suspensions of various densities in approved test tubes 3 2 User profile This device may only be operated by trained specialist staff They must have carefully read the operating manual and be familiar with the function of the device jenuew Burjeuado 3 3 Application limits 3 3 1 Declaration concerning the ATEX directive 94 9 EC A Risk of explosion LA Do not operate the device in areas where work is completed with explosive substances DANGER Do not use this device to process any explosive or highly reactive substances gt Do not use this device for processing any substances which could generate an explosive atmosphere Due to its design and the environmental conditions inside the device the Centrifuge 5427 R is not suitable for use in a potentially explosive atmosphere The device
2. Operating manual 4 Rinse the rotors and accessories thoroughly with distilled water Rinse the rotor bores of fixed angle rotors particularly thoroughly Do not immerse the rotor in liquid as liquid can get trapped inside the cavities 5 Place rotors and accessories on a cloth to dry Place fixed angle rotors with the rotor bores facing downwards to allow the bores to also dry Correctly reinsert the rotor lid sealing ring in the clean and dry groove Clean the rotor cone with a soft dry and lint free cloth Do not lubricate the rotor cone Inspect the rotor cone for damage o No 9 Place the dry rotor onto the motor shaft 10 Tighten the rotor nut firmly by turning it clockwise with the rotor key 11 Leave the rotor lid open when the rotor is not being used 6 3 Additional service instructions for Centrifuge 5427 R gt Empty and clean the condensation water tray regularly and especially after liquid spillage in the rotor chamber gt Regularly free the rotor chamber ice formations via thawing by leaving the centrifuge lid open or carrying out a short temperature control run at approx 30 C gt Leave the centrifuge lid open when not in use for a long period Residual moisture can escape The lid spring is relieved gt Wipe up condensate in the rotor chamber using a soft absorbent cloth Remove dust deposits from the ventilation slits of the centrifuge using a brush or swab at the latest every six months
3. Temperature Soft ramp Rotor accelerates and brakes slowly No symbol fast acceleration and braking of rotor g force rcf or speed rpm see Rotors on p 11 Status of centrifuge f Centrifuge lid unlocked m Centrifuge lid locked m Flashing Centrifuging in progress Display of centrifugation speed rcf g force relative centrifugal acceleration rpm speed revolutions per minute Status of speaker d Speaker switched on Nul A Speaker switched off 5 2 Menu navigation Proceed as follows to change settings in the device menu Open the menu Select the desired menu item Confirm your selection Select the setting of the corresponding parameters Confirm the changed setting The BACK menu item belonging to the first menu level appears Exit the menu 6000090 0 To exit the second menu level without changing a parameter select the BACK menu item and confirm with menu enter Centrifuge 5427 R Operating manual 5 3 Menu Tab 1 Menu structure of the Centrifuge 5427 R Menu level 1 M 1 Menu level 2 M 2 Display SOFT On Rotor accelerates and brakes slowly Soft ramp decreases acceleration speed and OFF Rotor accelerates and brakes rapidly braking speed Disabled with short spin centrifugation RAD 0 2ML Select the radius according to the adapter used For the internal conversion of speed rpm t
4. centrifuging at maximum g force rcf or speed rpm and maximum load 45 rri Z jenuew burje19d0 Centrifuge 5427 R Operating manual 9 5 Acceleration times and deceleration times for the Centrifuge 5427 R according to DIN 58 970 EN Rotor Acceleration time Deceleration time Mains voltage 5 230 V 120 V 100 V E FA 45 12 17 Without soft ramp Acceleration time 27 s 29s 295 Iz Deceleration time 24 s 25s 25s E With soft ramp Acceleration time 36 s 36 s 40s E Deceleration time 36 s 36 s 37s E FA 45 24 11 Without soft ramp Acceleration time 18 s 19s 19s Deceleration time 18s 19s 19s With soft ramp Acceleration time 27s 28s 29s Deceleration time 29 s 295 30s FA 45 24 11 Kit Without soft ramp Acceleration time 21 s 22s 22s Deceleration time 18s 19s 19s With soft ramp Acceleration time 29 s 295 31s Deceleration time 29 s 28s 29s FA 45 30 11 Without soft ramp Acceleration time 21 s 22s 22s Deceleration time 18s 19s 19s With soft ramp Acceleration time 30 s 29s 32s Deceleration time 30 s 30s 31s F 45 30 11 Without soft ramp Acceleration time 21 s 22s 22s Deceleration time 18s 19s 19s With soft ramp Acceleration time 29s 29s 315s Deceleration time 29s 29s 31s FA 45 48 11 Without soft ramp Acceleration time 1285 295 295 Deceleration time 22 s 23s 23s With soft ramp A
5. Centrifuge 5427 R Operating manual Register your instrument www eppendorf com myeppendoi rf wg eppendorf eppendorf Copyright 2012 Eppendorf AG Hamburg No part of this publication may be reproduced without the prior permission of the copyright owner Trademarks Eppendorf and the Eppendorf logo are registered trademarks of Eppendorf AG Hamburg Germany Microtainer is a registered trademark of Becton Dickinson Franklin Lakes NJ USA Trademarks are not marked in all cases with or 9 in this manual Operating manual e Umm 5 Declarations and Certificates ccce RR RR RR rr 216 5409 900 021 01 122012 A detailed description of these figures can be found in the Product description and Operation chapters 1 Centrifuge lid Monitoring glass Display Control panel 2 4 Condensation water tray only 6 Emergency release 8 Mains power switch 3 5 7 USB port only for Technical Service 9 Fuse holder 10 Power connection socket 11 Name plate 500 w2nnnds time rpm rcf temp nn lt temp Y speed RE 11 10 9 8 7 6 1 Call and select the menu parameters 2 Switch display of centrifugation speed rpm rcf 3 Display 4 Short spin centrifugation 5 Release lid 6 Start and stop centrifuga
6. First switch off the device and remove the power plug 6 4 Glass breakage When using glass tubes there is a risk of glass breakage in the rotor chamber The resulting glass splinters are swirled around in the rotor chamber during centrifugation and have a sandblasting effect on the rotor and accessories The smallest glass particles become lodged in the rubber parts e g the motor guide the rotor chamber seal and the rubber mats of adapters NOTICE Glass breakage in the rotor chamber Glass tubes in the rotor chamber may break if the g force is too high Broken glass can damage the rotor accessories and samples Please note the manufacturer s information on the recommended centrifugation parameters load and speed Effects of glass breakage in the rotor chamber Fine black metal abrasion in the rotor chamber in metal rotor chambers The surfaces of the rotor chamber and accessories are scratched The chemical resistance of the rotor chamber is reduced Contamination of samples Wear on rubber parts How to proceed in case of glass breakage 1 Remove all splinters and glass powder from the rotor chamber and accessories 2 Thoroughly clean the rotor and rotor chamber Thoroughly clean the bores of the fixed angle rotors in particular 3 If required replace the adapters to prevent any further damage 4 Regularly check the rotor bores for deposits and damage Centrifuge 5427 R Operating manual
7. Loading a fixed angle rotor A CAUTION Risk of injury due to asymmetric loading of a rotor Load rotors symmetrically with identical tubes gt Only load adapters with suitable tubes gt Always use the same type of tubes weight material density and volume gt Check symmetric loading by balancing the adapters and tubes used with scales jenuew Burjeaado CAUTION Risk from damaged or overloaded tubes When loading the rotor observe the safety precautions on dangers as a result of overloaded or damaged tubes see Warnings for intended use on p 19 0 Use matching rotor lids Fixed angle rotors may only be operated with the appropriate rotor lid in each case The rotor name on the rotor must be the same as the name on the rotor lid To carry out an aerosol tight centrifugation an aerosol tight rotor label red ring and the corresponding aerosol tight rotor lid label aerosol tight and red lid screw must be used The device automatically detects imbalances during operation and stops the run immediately with an error message and a signal tone gt Check the load balance the tubes and restart the run To load the rotor proceed as follows 1 Check the maximum payload adapter tube and contents for each rotor bore The information about this can be found on every rotor and in this operating manual see Rotors on p 11 2 Load rotors and adapters only with the tubes i
8. jenuew Burjeuado WARNING T gt Always open the centrifuge lid completely to prevent it from falling 1 Check the correct attachment of the rotor and rotor lid 2 Press the centrifuge lid down until it is gripped by the lid latch The lid will be automatically closed and the locking mechanism can be clearly heard The open key lights up blue The display shows the symbol 5 5 Cooling The centrifuge cools or maintains the set temperature is the following requirements are met The centrifuge is switched on The centrifuge lid is closed Only with continuous cooling The set temperature is lower than the ambient temperature Ci If the rotor stops continuous cooling cooling is slower than during centrifugation or a temperature control run 5 5 1 Set the temperature 1 To set the temperature use the temp arrow keys to select a temperature between 11 C and 40 C 2 Set the run time and g force rcf or speed rpm Press the start stop key to start the centrifugation The temperature can be changed during centrifugation 5 5 2 Temperature display Temperature display if the rotor stops Set temperature Temperature display during centrifugation Actual temperature 5 5 3 Temperature monitoring After the set temperature has been reached the centrifuge reacts to temperature deviations during centrifugation as follows Deviation from target temperature Action 3 C Temperature display flashes 5 C Display sho
9. used in FA 45 48 11 F 45 48 11 FA 45 30 11 F 45 30 11 FA 45 24 11 FA 45 24 11 Kit for 1 PCR tube 0 2 mL max 6 mm set of 6 for 1 sample tube 0 4 mL max 6 mm set of 6 pieces for 1 sample tube 0 5 mL max 6 mm or 1 Microtainer 0 6 mL max Y 8 mm set of 6 Centrifuge 5427 R Operating manual 10 2 3 Other accessories Order no Order No Description international North America Rotor key 5416 301 001 022634305 Standard 5409 850 083 Tray for condensation water 10 3 Fuses Order no Order No Description international North America Fuse 5301 850 249 022654403 4 A 230 V 2 pieces 5427 850 341 022654381 8 0 AT UL 120 V 100 V 2 pieces 5811 352 006 022664107 10 0 AT UL 100 V 2 pieces 49 rri Z jenuew burje19d0 rri Z Operating manual 50 Centrifuge 5427 R Operating manual gt Te Ms PA i A re CS vet A T i Z UN EG Konformitatserklarung VETE EC Conformity Declaration EOS tes Das bezeichnete Produkt entspricht den einschl gigen grundlegenden Anforderungen der e ti aufgef hrten EG Richilinian und Norman Bei einer nicht mit uns abgestimmten nderung des NT te Produktes oder einer nicht bestimmungsgem en Anwendung verliert diese Erkl rung ihre G ltigkeit ES The product named below fuifills the relevant fundamental requirements of gt l the EC directives and standards listad In the case of unauthorized
10. 6 2 Centrifuging in continuous operation RR eee 34 5 6 3 Short spin centrifugation 26 0 eee mr 34 5 6 4 Setting the centrifugation radius lile 35 5 6 5 Alter centrifugation areas aatia de Erwin Pie hiring 35 5 7 Standby mode cssc dk eee enc aa to ie a a See eh aed d 35 Malntenance oia ai a a a a a aa a aa a 36 6 1 Prepare cleaning disinfection co ocoooocoorocor on anne 36 62 Gleaning disinfectioni 4 anregen a as a 36 6 2 1 Cleaning and disinfecting the device aa 37 6 2 2 Cleaning and disinfecting the rotor aa 37 6 3 X Additional service instructions for Centrifuge 5427 R tee 38 64 Glass br aKaqe sci sensere muet ana de aa Ae Ma de eee cede 38 6 5 Replacing fuses sc eae EIQUE Quid bh E LI een 39 6 6 Decontamination before shipment RR nenn 39 Troubleshooting zu suo greg ark eR Rie ee E EEDA 40 7 1 General errors sepu Res ea ee he Ter TUR aser bee 40 7 2 Error messages sod vede peris SR eeu Roe xac tes Rcg Rus Rr Ho Rus cecus echec fedi beau e RR QURE 41 7 3 Emergency release 5s eoe Sb tabaci eee ia exis sh anite ieri 42 Transport storage and disposal oococcccccn hh hn hh hn 43 8 1 TRANS POL iu u cU TP 43 8 2 Storage s rasanten dd e E st s wis 43 8 3 Disposal ke ad dh Ed bd ES e de ER Mad e RR 43 Technical data cu nda ara ir A A a eek 44 9 1 POWerSUDDIY sirsie kap di de ot ee ER PURI A DEL e eee PMEMH 44 92 Ambient Conditions ad peu e x ha
11. 6 5 Replacing fuses Electric shock AN Switch off the device and disconnect the power plug before starting maintenance or cleaning work DANGER The fuse holder is located between the mains connection socket and the mains power switch 1 Disconnect the power plug 2 Press the upper and lower end of the plastic springs 1 together and pull the fuse holder 2 fully out 3 Replace faulty fuses and reinsert the fuse holder Make sure that the guiding rail 3 is positioned correctly 6 6 Decontamination before shipment If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following A Risk to health from contaminated device IN 1 Observe the notes on the decontamination certificate You find it as a PDF file on our website WARNING www eppendorf com decontamination 2 Decontaminate all the parts you would like to dispatch 3 Include the fully completed decontamination certificate in the package 39 rri Z jenuew Burjeaado Centrifuge 5427 R Operating manual EN 7 Troubleshooting If you cannot remedy an error with the recommended measures please contact your local 1 i Eppendorf partner The contact address can be found online at www eppendorf com worldwide E 7 1 General errors 2 Symptom message Cause Remedy n a No display
12. Aerosol tight fixed angle rotor for 24 tubes Max load adapter 24 x 3 75 g tube and contents Vessels Vessel Adapters Adapter base Max g force Capacity Order no Tube diameter Max speed Vessels per adapter rotor international Centrifugation radius Vessel u 25 001 x g i 1 5 mL 2 0 mL Q 11mm 16 220 rpm 24 8 5 cm PCR tube conical 18825xg 1 0 2 mL Y 6 mm 16 220 rpm 5425 715 005 v Vessel conical 25 001 x g 0 4 mL 6mm 16 220 rpm 1e 5425 717 008 aoc Vessel open 21 766 xg i 0 5 mL Y 8 mm 16 220 rpm 1 24 5425 716 001 TA em Microtainers open 25001 xg N 0 6 mL Y 8 mm 16 220 rpm Wes 5425 716 001 8 5 cm Centrifuge 5427 R Operating manual 2 4 3 Rotor FA 45 24 11 Kit Max g force 19090 x g Rotor FA 45 24 11 Kit Max speed 13 200 rpm Aerosol tight fixed angle rotor for 24 tubes Max load adapter 24 x 3 759 tube and contents Vessels Vessel Adapters Adapter base Max g force Capacity Order no Tube diameter Max speed Vessels per adapter international Centrifugation rotor radius Vessel 19 090 xg i 1 5 mL 2 0 mL 11 mm 13 200 rpm 24 9 8 cm PCR tube conical 15 000 xg j 0 2 mL 6 mm 13 200 rpm ia 5425 715 005 TIR Vessel conical 19 090 x g l 0 4 mL 6 mm 13 200 rpm MES 5425 717 008 Sach Vessel open 16 948 x g i 0 5 mL 8 mm 13 200 rpm i 5425 716 001 acom Microtainers open 19 090 x g N 0 6
13. Delivery package sia d va ee he eines di Se EET erred We ean ine 10 2 3 tug PR dl D e ho e 10 2 4 ROOS sities a ad Be Bin hehe Dh da d etd aba en 11 2 4 1 Rotor FA453 29173 pa ip bee dei P B E 11 2 4 2 RotorFA 45 24 11 c nane IL eR rri ede eee DG Gd pL ad 12 2 4 3 Rotor FA 45 24 11 Kit 0 tet rn 13 2 4 4 Rotors FA 45 30 11 and F 45 30 11 2 0 eee eee 14 2 4 5 Rotors FA 45 48 11 and F 45 48 11 2 eee tenes 15 2 4 6 Rotor F 45 48 5 PCR 2 0 eet teen eee 16 2 4 7 Rotor S 24 11 AT iiu cun cack rece nee need eee RD pee ee ade nee ede A 16 Safety oi cie dg deed Sine Mew Dee eR eee eee eR ee 17 3 1 Intended use od bee LES Pe PUEDE LU i perte d aps 17 3 2 User protile ao her RR per p EE Rete ade Rowe ea ee XUI a AE M EAS 17 3 3 Application imiss iun cite RR ee er RU RR de eee peer er iun ache ee be Pads pt 17 3 3 1 Declaration concerning the ATEX directive 94 9 EC lille eese 17 3 3 2 Maximum service life for accessories lille 17 3 4 Information on product liability see 18 3 5 Warnings for intended use e 19 3 5 1 Personal injury or damage to the equipment aa 19 3 5 2 Incorrect handling of the centrifuge llle 20 3 5 3 Incorrect handling of the rotors ett 20 3 5 4 Extreme strain on the centrifuging tubes 21 3 5 5 Aerosol tight centrifugation ia eee e n 22 3 6 Safety instructions located on the device 0 ccc cette tenes 22 Installation wird nema ideale as ne a R
14. Inner row Capacity Order no Tube diameter Max speed Centrifugation radius Outer row Inner row Vessel 18213 xg 16049xg 1 5 mL 2 0 mL 11mm 12 700 rpm 48 10 1 cm 8 9 cm PCR tube conical 14 426 x g 12262 x g 0 2 mL 5425 715 005 emm 12 700 rpm 1 48 8 cm 6 8 cm Vessel conical 18213 xg 16049 x g 0 4 mL 6 mm 12 700 rpm 5425 717 008 1 48 10 1 cm 8 9 cm Vessel open 16 229 x g i 14 065 x g 0 5 mL Y 8 mm 12 700 rpm 5425 716 001 1 48 9 cm 7 8 cm Microtainers open 18213xg N 16049 x g 0 6 mL 8 mm 12 700 rpm 5425 716 001 1 48 10 1 cm 8 9 cm 15 rri Z jenuew burje19d0 rri Z Centrifuge 5427 R Operating manual Operating manual Tubes per adapter rotor 2 4 6 Rotor F 45 48 5 PCR Rotor F 45 48 5 PCR Max g force 11710xg Fixed angle rotor for 48 PCR tubes Max speed 10 500 rpm Max load tube and 48 x 0 43g contents Tubes Tube Adapter Adapter base Max g force Capacity Tube diameter Max speed Centrifugation radius conical 11710xg i 0 2 mL 6 mm 10 500 rpm 48 9 5 cm PCR strips conical 11710xg 0 2 mL 6 mm 10 500 rpm 6 x 8 9 5 cm 2 4 7 Rotor S 24 11 AT This rotor is only intended for use with 1 5 mL 2 0 mL tubes The following tubes must not be used in this rotor Adapters for 0 2 mL 0 4 mL 0 5 mL and 0 6 mL tubes and the corresponding tubes Spin columns
15. Please remove all materials and objects from this area Damage from overheating Do not install the device near to any heat sources e g heating drying cabinet NOTICE Do not expose the device to direct sunlight gt Ensure unobstructed air circulation Keep free a clearance of at least 30 cm 11 8 in around all ventilation grilles Select the location for the device according to the following criteria Suitable power connection as per the name plate 230 V 120 V 100 V Stable horizontal and resonance free lab bench Weight of the device 30 0 kg 66 14 Ib A well ventilated environment which is protected from direct sunlight to prevent the device from heating up more 4 2 Preparing installation Prerequisites The weight of the centrifuge is 30 0 kg 66 14 Ib For unpacking and installation the assistance of another person is required Perform the following steps in the sequence described Open the box Remove the accessories The centrifuge must be lifted from the box by two people Remove the transport securing device from the sides Place the device on a suitable lab bench ar ON gt O Do not use the opening for the condensation water tray as a handle 6 Remove the plastic sleeve 23 rri Z jenuew burje19d0 Centrifuge 5427 R Operating manual 4 3 Installing the instrument EN Prerequisites The device is on a suitable lab bench Condensation can form inside the dev
16. display and operating controls A diagram of the operating controls and display can also be found on the front fold out page 1 2 3 4 5 pe z Qe nnn e Q Ho ILI time temp speed Qo rpm Q Psi fast Es EN 11 10 9 8 7 6 Fig 2 Operator panel of the Centrifuge 5427 R 1 Call and select the menu parameters 2 Switch display of centrifugation speed see Menu navigation on p 26 rpm or rcf 3 Display 4 Short spin centrifugation see Short spin centrifugation on p 34 5 Release lid 6 Start and stop centrifugation 7 Set speed of centrifugation 8 Settemperature 9 Setcentrifugation time 10 Start FastTemp temperature control run see Start FastTemp on p 32 11 Select menu item see Menu navigation on p 26 Please also read the precise description of the individual menu functions see p 27 25 rri Z jenuew burje19d0 rri Z Operating manual 26 Centrifuge 5427 R Operating manual Fig 3 1 2 3 4 E 9 Display of the Centrifuge 5427 R _ IN dure IO iie uH 5 6 7 A LILILILI 8 1 Centrifugation time Key lock oKey lock Centrifugation parameters cannot be accidentally changed wu No key lock Centrifugation parameters can be changed Start of run time ATSET function Start of run time after reaching 95 of the specified g force rcf or speed rpm Immediate start of run time
17. for repairs The procedure described in the following chapter applies to the cleaning as well as to the disinfection or decontamination The table below describes the steps required on top of this Cleaning Disinfecting decontamination 1 Use a mild cleaning fluid to clean the accessible surfaces of the device and the accessories Carry out the cleaning as described in the following chapter 1 Choose the disinfection method which corresponds to the legal regulations and guidelines in place for your range of application For example use alcohol ethanol isopropanol or alcohol based disinfectants Carry out the disinfection or decontamination as described in the following chapter Then clean the device and the accessories 0 If you have any further questions regarding the cleaning and disinfection or decontamination or regarding the cleaning fluid to be used contact the Eppendorf AG Application Support The contact details are provided on the back of this manual 6 2 Cleaning disinfection A DANGER Electric shock as a result of penetration of liquid Switch off the device and disconnect the power plug before starting cleaning or disinfection work Do not allow any liquids to penetrate the inside of the housing Do not spray clean spray disinfect the housing Only plug the device back in if it is completely dry both inside and outside NOTICE Damage from
18. modifications to the product or an unintended use this declaration becomes invalid cS re CI Produktbezeichnung Product Centrifuge 5427 R einschlie lich Zi including accessories Laboratory Centrifuge t C C ET 1 ch E Einschiagige EG ie Relevant EC directive s c CS Te 2006 95 EG EN 61010 1 EN 61010 2 20 97 23 EG ce gt 4 re 2004 108 EG 00 3 2 EN 61000 3 3 E ES te 98 79 EG EN 14971 EN 61010 2 101 EN 62366 EN 18113 3 c ets i CT te we C Vorstand Board of Management Projektmanagement Project Management PX are 25 05 2012 de C Hamburg Oate Health Protection Agency Microbiology Services Health Protection Porton Down Salisbury Wiltshire u SP4 0JG Certificate of Containment Testing Containment Testing of Rotor FA 45 48 11 5409 710 109 00 in the Eppendorf 5427 R Bench Top Centrifuge Report No 200 12 A Report Prepared For Eppendorf AG Hamburg Germany Issue Date 12 September 2012 Test Summary Rotor FA 45 48 11 5409 710 109 00 was containment tested in the Eppendorf 5427 R bench top centrifuge using Annex AA of IEC 1010 2 20 The sealed rotor was shown to contain a spill within the centrifuge Report Written By Report Authorjsed By PU Un Name Miss Anna Moy Namg Mrs Sara Speight Title Biosafety Scientist Title Senior Biosafety Scientist Health Protection Agency nN Microbiology Services Porton Dow
19. run time that can be set must be 2 min longer than the elapsed time O When using adapters you can adjust the radius 5 6 2 Centrifuging in continuous operation Perform the following steps in the sequence described 1 Use time to set the continuous run The continuous run function can be set above 9 59 h or below 10 s The timer shows oo to indicate continuous operation 2 Use temp to set the temperature 3 With speed set the g force rcf or speed rpm 4 Press the start stop key to start the centrifugation The msymbol blinks in the display when the rotor is running 5 Press the start stop key to end the centrifugation During the braking process centrifugation time flashes in the display When the rotor stops a signal tone is heard The centrifuge lid remains closed to maintain the sample temperature You can open it by pressing the open key 5 6 3 Short spin centrifugation Prerequisites In the SHORT menu the user sets whether the short spin centrifugation runs with the maximum g force rcf or speed rpm of the inserted rotor MAX or with a freely selected speed SET The short spin centrifugation runs as long as the short key is pressed 1 Only with short spin centrifugation with a set speed set the g force rcf or speed rpm using the speed arrow keys 2 Use the temp arrow keys to adjust the temperature 3 Start short spin centrifugation hold the short key pressed down The asymbol blinks
20. 0 mL PCR strips Microtainers 0 6 ml Spin columns 1 5 mL 2 0 mL The Centrifuge 5427 Rhas a temperature function for centrifugation at temperatures of 11 C to 40 C The FastTemp function is used to start a temperature control run without samples in order to quickly bring the rotor chamber to the set temperature Centrifuge 5427 R Operating manual 2 4 Rotors Ci Eppendorf centrifuges may only be operated with rotors that are intended for use with the corresponding centrifuge gt Only use rotors which are marked with the name of the centrifuge e g 5427 R 2 4 1 Rotor FA 45 12 17 Max g force 20 598 x g Rotor FA 45 12 17 Max speed 14 000 rpm Aerosol tight fixed angle rotor for 12 reaction tubes Max load adapter 12x9 5g tube and contents Vessels Vessel Adapters Adapter base Max g force Capacity Order no Tube diameter Max speed international Vessels per adapter Centrifugation rotor radius Vessel 20 598 x g 5 mL Q 17 mm 14 000 rpm 4 2 9 4 cm Vessel open 17 530 x g 1 12 8 0 cm HPLC vessel S flat 16215 xg 1 12 7 4 cm Cryo tube flat 18 188 xg 1 0 mL 2 0 mL z 13 mm 14 000 rpm 142 5820 769 009 8 3 cm rri Z jenuew burje19d0 EN Operating manual Centrifuge 5427 R Operating manual 2 4 2 Rotor FA 45 24 11 Max g force 25001 xg Rotor FA 45 24 11 i Max speed 16 220 rpm
21. 004 Rotor FA 45 30 11 aerosol tight aluminum angle 45 30 places max tube diameter 11 mm incl rotor lid aluminum 5409 707 000 5409707000 Rotor lid for FA 45 30 11 aerosol tight aluminum 5820 762 004 5820762004 Seal for rotor lid FA 45 30 11 5427 R 5430 5430 R Set of 5 pieces 5409 708 007 5409708007 Rotor F 45 30 11 aluminum angle 45 30 places max tube diameter 11 mm incl rotor lid polypropylene 5409 709 003 5409709003 Rotor lid for F 45 30 11 Polypropylene 5409 710 001 5409710001 Rotor FA 45 48 11 aerosol tight aluminum angle 45 48 places max tube diameter 11 mm incl rotor lid aluminum 5409 711 008 5409711008 Rotor lid for FA 45 48 11 aerosol tight aluminum 5409 718 002 5409718002 Seal for rotor lid FA 45 48 11 5427 R 5430 5430 R set of 5 pieces 5409 712 004 5409712004 Rotor F 45 48 11 aluminum angle 45 48 places max tube diameter 11 mm incl rotor lid polypropylene 5409 713 000 5409713000 Rotor lid for F 45 48 11 Polypropylene 47 rri Z jenuew burje19d0 rri p Operating manual 48 Centrifuge 5427 R Operating manual Order no international Order No North America Description 5409 704 001 5409704001 Rotor FA 45 24 11 Kit aerosol tight aluminum angle 45 24 places max tube diameter 11 mm incl rotor lid 5409 705 008 540970
22. 50 050 North America 022634330 Contaminated grooves and pegs prevent a uniform swinging of the bucket 2 Hang the buckets into the rotor All rotor positions must be loaded with carriers e Check that all carriers are hanging properly and can swing freely gt Check the maximum payload adapter tube and contents for each bucket The details on the maximum payload can be found on the rotor and in this operating manual see Rotors on p 11 5 Load the buckets symmetrically Fig 4 Incomplete but symmetric loading of the buckets The pegs of each bucket must be uniformly loaded The supply shown on the right side is incorrect because it does not allow the bucket to properly swing out Centrifuge 5427 R Operating manual 5 4 6 Closing the rotor lid rri Z 1 Only QuickLock rotor lid check that the external sealing ring correctly fits in the groove 2 Vertically fit the rotor lid on the rotor 3 Turn the rotor lid screw clockwise to seal the rotor Only QuickLock rotor lid Turn the rotor lid screw clockwise as far as it will go until an audible click can be heard The rotor is not properly closed until an audible click is heard 5 4 7 Closing the centrifuge lid Crushing of the fingers with the centrifuge lid A When opening or closing the device lid do not reach between the lid and device or into the latching mechanism of the lid
23. 5008 Rotor lid for FA 45 24 11 Kit aerosol tight aluminum 5820 767 006 5820767006 Seal for rotor lid FA 45 24 11 Kit 5427 R 5430 5430 R FA 45 48 11 FA 45 20 17 5804 5804 R 5810 5810 R Set of 5 pieces 5409 700 006 5409700006 Rotor FA 45 12 17 aerosol tight aluminum 45 angle 12 places max tube diameter 17 mm incl rotor lid aluminum 5409 701 002 5409701002 Rotor lid for FA 45 12 17 aerosol tight aluminum 5409 716 000 5409716000 Seal for rotor lid FA 45 12 17 5427 R set of 5 pieces 5409 714 007 5409714007 Rotor F 45 48 5 PCR aluminum angle 45 48 places max tube diameter 6 mm 5409 715 003 5409715003 Rotor S 24 11 AT aerosol tight steel angle 90 24 places max tube diameter 11 mm incl rotor lid aluminum 5409 720 007 5409720007 Rotor lid for S 24 11 AT aerosol tight aluminum 5409 721 003 5409721003 Tube holder for S 24 11 AT for 4 x 1 5 mL 2 0 mL Eppendorf tubes set of 2 pieces 5409 719 009 5409719009 Seal for rotor lid S 24 11 AT 5427 R 5430 5430 R set of 5 pieces 10 2 2 Adapters Aerosol impermeability tested and certified by the Centre of Emergency Preparedness and Response Health Protection Agency Porton Down UK Order no international Order No North America Description 5425 715 005 5425 717 008 5425 716 001 022636260 022636243 022636227 Adapter
24. EMC interference emission radio EN 61326 Class B 230 V interference FCC15 Class A 120 V 100 V EMC noise immunity EN 61326 Overvoltage category Il Fuses 4 A 230 V 8A 120 V 10 A 100 V Degree of pollution 2 9 2 Ambient conditions Ambience Only for use indoors Ambient temperature 10 C 40 C Relative humidity 10 75 non condensing Atmospheric pressure 79 5 kPa 106 kPa 9 3 Weight dimensions Dimensions Width 31 9 cm 12 6 in Depth 54 0 cm 21 3 in Height 25 4 cm 10 2 in Weight without rotor 30 0 kg 66 14 Ib Noise level lt 56 dB A The noise level was measured head on according to DIN EN ISO 3745 in an acoustic chamber with accuracy class 1 at a distance of 1 m to the device and at the height of the lab bench 44 Centrifuge 5427 R Operating manual 9 4 Application parameters Run time 10 s 9 59 h infinite co e 10 s 2 min can be set in increments of 10s 2 min 10 min can be set in increments of 30s 10 min 9 59 h can be set in increments of 1 min Temperature 11 C 40 C Relative centrifugal force 1xg 25001xg can be set in increments of 50 x g Speed 100 rpm 16 220 rpm can be set in increments of 50 rpm Maximum load 48 tube with 2 0 mL volume Maximum kinetic energy 9920 Nm Compulsory test log book in Germany No Permissible density of the material for 1 2 g mL
25. No mains power connection Check the mains power connection 2 No display Povver failure Check the mains fuse of the laboratory Lid of the device cannot Rotor is still running Wait for rotor to stop be opened Lid of the device cannot Power failure 1 Check the mains fuse of the laboratory be opened Device cannot be started Lid of the device is not closed Close the lid of the device Device shakes when it Rotor is asymmetrically loaded 1 Stop the device and load symmetrically starts up 2 Reboot device Centrifuge brakes during The short key was released briefly more than Press the short key continuously during a a short run centrifugation twice protective function for the drive short run centrifugation although the short key is pressed Temperature display Temperature deviation from the set value Check the settings flashes 3 C Wait until the target temperature has been reached Check unhindered air circulation through the air slots gt Thaw ice or switch off device and allow it to cool down 40 Centrifuge 5427 R Operating manual 7 2 Error messages Proceed as follows if the following error messages appear 1 Remove fault see remedy 2 If necessary repeat centrifugation Symptom message Cause Remedy ERR 1 Rotor not detected Check rotor gt If this error message appears again test with a different rotor Err 2 Electroni
26. ame RE s 23 4 1 Selecting theil cation a4 atii Fac e RR n eum AE ERE i d E ios i a Ebr E ea 23 4 2 Preparing installation lt lt eise eR mcer pe ES CEDE esa Euge eere dere da qd ed 23 4 3 Installing the instrument RR RR Im um 24 Operation pU 25 5 1 Overview of operating CONtTO S oooocooccoo hr 25 5 2 Menu navigation se n une a dh le ade 26 5 3 Lap RUMP 27 5 4 Preparing for centrifugation 0 00 aaa pn 28 5 4 1 Switching on the centrifuge RR een 28 5 4 2 Irisertirig INE rotor v era PALEIS ane ee 28 5 4 3 Automatic rotor detection 2 2 0 el m m 28 5 4 4 Replacing the rotor uci ER REI me WEA ee hl EAE mean 28 5 4 5 Loading NS TOt PDT 29 5 4 6 Closing Ihe rotor lid i eim cir eR ITA ee Dee a nd 31 5 4 7 Closing the centrifuge lid teen RII rn 31 rri Z jenuew burje19d0 rri Z Operating manual Centrifuge 5427 R Operating manual 10 II 1510 E E ME LE Moa E aye LULA etal 31 5 5 1 Set the temperature rmm 31 5 5 2 Temperature display o va harami ek xe sage ne x ew nadie a a mann k OREG CERE E RR ne 31 5 5 3 Temperature monitoring aa ce m hne 31 5 5 4 Start FastTemp i esce hes abe bb KEY a eae eed ae Ded cis 32 5 5 5 Continuous cooling s di oie RR etait dee dee Pe eee ee eh eee ee RR 32 5 5 6 Endless operation of continuous Cooling aa 33 56 Centituging rca ee pee acd pen dori en Paus dr cee ma ddr 33 5 6 1 Centrifuging with preset time liliis I 33 5
27. be E MARE do a REGE Pads Rau E RR ERU RUE A 44 9 3 Weight dimersioris 2 422222 e eI E keke ee bee x ee or aen Be ER NE RU CAES 44 9 4 Application parameters lsssseeseeeee e RII RA I I I mum meme ns 45 9 5 Acceleration times and deceleration times for the Centrifuge 5427 R according to DIN 58 970 46 Ordering information dua ca k b ei k av A A RO DURS Edi ein d 47 10 1 Centrifuge 5427 Ro ete rr 47 10 2 Accessories i bob dade een erbe eee deed bb ase dee r S 47 10 21 Rotors and rotor ll ses sasie ges Rex Rakes E E bleed G s end E Y eee 47 10 2 2 Adapters c sodes eee ec ERE ee Ba Da 48 10 2 8 Other accessories aa 49 Centrifuge 5427 R Operating manual 1 User instructions 1 1 Using this manual Read this operating manual completely before using the device for the first time Please also note the operating instructions for the accessories if applicable gt This operating manual is part of the product Thus it must always be easily accessible gt Enclose this operating manual when transferring the device to third parties gt If this manual is lost please request another one You will find the current version on our webpage www eppendorf com worldwide 1 2 Danger symbols and danger levels The safety instructions in this manual appear with the following danger symbols and danger levels 1 2 1 Danger symbols A Biohazard A Explosion d b po A Electric
28. cceleration time 36 s 36 s 39 s Deceleration time 35 s 35s 35s F 45 48 11 Without soft ramp Acceleration time 28 s 29s 29s Deceleration time 22 s 23s 23s With soft ramp Acceleration time 36 s 36 s 39 s Deceleration time 35 s 35s 35s F 45 48 PCR Without soft ramp Acceleration time 11 s 12s 12s Deceleration time 12 s 13s 13s With soft ramp Acceleration time 19 s 19s 21s Deceleration time 20s 19s 21s S 24 11 AT Without soft ramp Acceleration time 18 s 18s 18s Deceleration time 17 s 17s 17s With soft ramp Acceleration time 26 s 26s 28s Deceleration time 27s 215 28s Centrifuge 5427 R Operating manual 10 Ordering information 10 1 Centrifuge 5427 R Order no international Order No North America Description 5409 000 012 5409 000 136 5409 000 039 5409000136 Centrifuge 5427 R without rotor 230 V 50 Hz 60 Hz 120 V 50 Hz 60 Hz 100 V 50 Hz 60 Hz 10 2 Accessories 10 2 1 Rotors and rotor lids Order no international Order No North America Description 5409 702 009 5409702009 Rotor FA 45 24 11 aerosol tight aluminum angle 45 24 places max tube diameter 11 mm incl rotor lid aluminum 5409 703 005 5409703005 Rotor lid for F 45 24 11 aerosol tight aluminum 5409 717 006 5409717006 Seal for rotor lid FA 45 24 11 5427 R FA 45 16 17 5430 5430 R Set of 5 pieces 5409 706 004 5409706
29. cs fault Switch centrifuge off and back on again after gt 20 s NO RPM ERR 3 Error in speed measuring system Leave the device switched on until the error message disappears 10 s or 6 min Err 5 Prohibited opening of lid or lid switch is 1 Wait for rotor to stop defective during a run Err 6 Error in the drive electronics gt Repeat the run Drive overheated gt If this error message appears again switch centrifuge off and back on again after gt 20 s Allow the drive to cool down for at least 15 min Err 7 Major deviation in the speed control 1 Wait for rotor to stop 2 Tighten the rotor Err 8 Drive fault 1 Wait for rotor to stop 2 Repeat the run ERR 9to ERR 14 Electronics error Switch centrifuge off and back on again after gt 20 s IMBAL ERR 15 Rotor is asymmetrically loaded Load the rotor symmetrically and balance ERR 16to ERR 17 Electronics error Switch centrifuge off and back on again after gt 20 s Err 18 Temperature deviation from set value in the Check the settings rotor chamber 5 C Check unhindered air circulation through the air slots gt Thaw ice or switch off device and allow it to cool down Err 19 Condenser overheated Check unhindered air circulation through the air slots Allow device to cool down Err 20 Temperature sensor in rotor chamber is Switch centrifuge off and back on again faulty a
30. dby mode activated Switch the standby mode on or off OFF Standby mode deactivated If the centrifuge has not been used for 15 min it will switch to standby EP then appears in the display Press a key or close the centrifuge lid to exit the standby mode The Back menu item can also be found in both menu levels Back in menu level 2 return to menu level 1 Back in menu level 1 exit menu 27 rri Z jenuew burje19d0 rri p Operating manual 28 Centrifuge 5427 R Operating manual 5 4 Preparing for centrifugation 5 4 1 Switching on the centrifuge Switch the centrifuge on using the mains power switch The centrifuge lid opens automatically after switching on using the mains power switch The parameter settings of the last run are displayed 5 4 2 Inserting the rotor 1 Fit the rotor vertically on the motor shaft 2 Insert the supplied rotor key into the rotor nut 3 Turn rotor key clockwise until the rotor nut is firmly tightened 5 4 3 Automatic rotor detection Ci The centrifuge has automatic rotor detection It detects a newly inserted rotor and displays the name of the rotor for 2 s g force rcf and speed rpm are automatically limited to the maximum permitted value for the rotor gt Manually turn the rotor counterclockwise to trigger rotor detection The name of the rotor appears in the display The g force rcf and speed rpm are automatically limited to the maximum permissib
31. e recommended by Eppendorf may LN J impair the safety functioning and precision of the device Eppendorf cannot be held liable or CAUTION accept any liability for damage resulting from the use of incorrect or non recommended accessories and spare parts or from the improper use of such equipment gt Only use accessories and original spare parts recommended by Eppendorf 19 rri Z jenuew burje19d0 rri p Operating manual 20 Centrifuge 5427 R Operating manual NOTICE Damage to device due to spilled liquids Switch the device off Disconnect the device from the power supply 3 Carefully clean the device and the accessories in accordance with the cleaning and disinfection instructions in the operating manual 4 Ifa different cleaning and disinfecting method is to be used contact Eppendorf AG to ensure that the intended method will not damage the device N Damage to electronic components due to condensation Condensation can form inside the device after the device has been moved from a cool to a warmer environment NOTICE l gt 5427 R Wait for at least 4 hours before connecting the device to the power supply Centrifuge 5427 R compressor damage after improper transport gt Only switch on the centrifuge 4 hours after installation NOTICE 3 5 2 Incorrect handling of the centrifuge NOTICE Damage from knocking against or moving the device during operation If th
32. e rotor bangs against the rotor chamber wall it will cause considerable damage to the device and rotor gt Do not move or knock against the device during operation 3 5 3 Incorrect handling of the rotors Risk of injury from improperly attached rotors and rotor lids gt Only centrifuge with rotor and rotor lid firmly tightened gt If unusual noises occur when the centrifuge starts the rotor or the rotor lid may not be WARNING Sie properly secured Immediately press the start stop key to stop centrifuging Risk of injury due to asymmetric loading of a rotor DA Load rotors symmetrically with identical tubes CAUTION gt Only load adapters with suitable tubes Always use the same type of tubes weight material density and volume gt Check symmetric loading by balancing the adapters and tubes used with scales N LA CAUTION Risk of injury from overloaded rotor The Centrifuge 5427 R is designed for the centrifugation of centrifugation material with a max density of 1 2 g mL at maximum speed and volume Please note the information on each rotor on the maximum load adapter tube and contents per rotor bore and or per bucket and do not exceed it Centrifuge 5427 R Operating manual Damage to rotors from aggressive chemicals Rotors are high quality components which withstand extreme stresses This stability can be NOTICE impaired by aggressive chemicals gt Avoid the use of agg
33. ensity of plastic tubes is reduced when organic solvents e g phenol chloroform are NOTICE used i e the tubes could become damaged gt Note the manufacturer s information on the chemical resistance of the tubes 21 rri Z jenuew burje19d0 rri Z Operating manual 22 Centrifuge 5427 R Operating manual 3 5 5 Aerosol tight centrifugation Risk to health due to limited aerosol tightness with incorrect rotor rotor lid combination AN Aerosol tight centrifugation is guaranteed only if the rotors and rotor lids intended for this purpose are used For fixed angle rotors the labeling always begins with FA swing bucket rotors are WARNING labeled with AT aerosol tight The aerosol tight rotors and rotor lids of this centrifuge are additionally marked with a red ring on the rotor and a red rotor lid screw gt For aerosol tight centrifugation always simultaneously use rotors and rotor lids which are marked as aerosol tight in the centrifuge intended for the corresponding purpose The details specifying in which centrifuge you may use the aerosol tight rotors and rotor lids can be found on the rotor and beginning from production date of October 2003 on the upper side of the rotor lid gt Only use aerosol tight rotor lids in combination with rotors which are marked on the rotor lid A Health hazard from limited aerosol tightness due to incorrect use N Autoclaving mechanical stresses and contaminatio
34. erate the emergency release WARNING To check look through the inspection glass in the centrifuge lid Ci Use the rotor key delivered with the Centrifuge 5427 R for the emergency release 1 Pull the mains power plug 2 Remove the plastic cover for the emergency release on the right side of the device see Fig 1 and Fig 2 Turn the plastic cover 90 counterclockwise using an appropriate tool e g screwdriver and remove it 3 Insert the centrifuge rotor key in the rear hexagonal opening until a noticeable resistance is felt 4 Turn the rotor key counterclockwise This will release the centrifuge lid 5 Open the centrifuge lid 6 Remove the rotor key or turn the plastic covers back on Turn the plastic cover using an appropriate tool e g screwdriver by 90 in a clockwise direction Centrifuge 5427 R Operating manual C 8 Transport storage and disposal 8 1 Transport Bodily injury due to lifting and carrying heavy loads The device is heavy Lifting and carrying the device can lead to back injuries The device must be transported by least two people CAUTION Use a transport aid e g dolly to transport the device longer distances gt Remove the rotor from the centrifuge before transport gt Use the original packaging for transport Air temperature Relative humidity Atmospheric pressure General transport 25 C 60 C 10 96 75 96 30 kPa 106 kPa A
35. ervice personnel who have been specifically authorized by Eppendorf Risk from incorrect supply voltage N gt Only connect the device to voltage sources that match the electrical requirements listed on m the name plate i Only use sockets with a protective earth PE conductor and suitable power cable A Damages to health due to infectious liquids and pathogenic germs AN gt When handling infectious liquids and pathogenic germs observe the national regulations the TIT biological security level of your laboratory the material safety data sheets and the manufacturer s application notes Use aerosol tight sealing systems for the centrifugation of these substances gt When working with pathogenic germs belonging to a higher risk group more than one aerosol tight bioseal must be used gt Wear personal protective equipment Consult the Laboratory Biosafety Manual Source World Health Organization Laboratory Biosafety Manual as amended for comprehensive regulations on the handling of risk group Il germs or biological materials Crushing of the fingers with the centrifuge lid gt When opening or closing the device lid do not reach between the lid and device or into the latching mechanism of the lid WARNING I f gt Always open the centrifuge lid completely to prevent it from falling A Poor safety due to incorrect accessories and spare parts The use of accessories and spare parts other than thos
36. es Salisbury Wiltshire SP4 OJG Certificate of Containment Testing Containment Testing of Rotor FA 45 24 11 Kit 5409 704 109 00 in the Eppendorf 5427 R Bench Top Centrifuge Report No 200 12 E Report Prepared For Eppendorf AG Hamburg Germany Issue Date 12 September 2012 Test Summary Rotor FA 45 24 11 Kit 5409 704 109 00 was containment tested in the Eppendorf 5427 R bench top centrifuge using Annex AA of IEC 1010 2 20 The sealed rotor was shown to contain a spill within the centrifuge Ay Written By Miss Anna Moy va Mot Report Authorised By Porton Down Salisbury en Wiltshire us SP4 OJG Health Protection Agency Microbiology Services Health Protection Certificate of Containment Testing Containment Testing of Rotor S 24 11 AT 5409 715 100 00 in the Eppendorf 5427 R Bench Top Centrifuge Report No 200 12 F Report Prepared For Eppendorf AG Hamburg Germany Issue Date 12 September 2012 Test Summary Rotor S 24 11 AT 5409 715 100 00 was containment tested in the Eppendorf 5427 R bench top centrifuge using Annex AA of IEC 1010 2 20 The sealed rotor was shown to contain a spill within the centrifuge Report Written By Report Authorised By Fu VA N i Name Miss Anna Moy Operating Manaj Evaluate your operating manual www eppendorf com manualfeedback eppendorf Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamb
37. fter 20 s Err 21 Temperature sensor on condenser is faulty Switch centrifuge off and back on again after gt 20 s Err 22 Electronics fault Switch centrifuge off and back on again after gt 20 s NO E FAN ERR 23 Electronic fan is faulty Switch centrifuge off and back on again after 20 s 41 rri Z jenuew burje19d0 rri Z Operating manual Centrifuge 5427 R Operating manual Symptom message Cause Remedy Err 24 Failure at the compressor Allow the centrifuge to cool down and repeat the run INT ERR 25 Power failure during a run Check the mains connection Err 28 Error during speed check Switch centrifuge off and back on again after gt 20 s Err 29 Set g force speed too high e g after a rotor Check the g force speed change see Automatic rotor detection on Repeat the run p 28 LID ERR 30 Centrifuge lid cannot be locked Try again to close centrifuge lid Centrifuge lid cannot be released Switch the centrifuge off and back on Press the open key If the error occurs repeatedly Switch off centrifuge gt Actuate emergency release see Emergency release on p 42 OFF Displayed after power off 7 3 Emergency release If the centrifuge lid cannot be opened you can activate the emergency release manually A Risk of injury from rotating rotor AN gt Wait until the rotor has come to a standstill before you op
38. he largest radius of the rotor used 5 6 5 After centrifugation Ci If start centrifuging immediately has not been used for 15 min it switches to standby EP appears in the display If the centrifuge is temporarily not used carry out the following steps Please also observe the care instructions see p 38 gt Turn the rotor nut counterclockwise using the supplied rotor key Remove the rotor by lifting it vertically gt Empty the condensation water tray gt Leave the centrifuge lid open 5 7 Standby mode The centrifuge switches to the standby mode when the following prerequisites are met Centrifuge lid is open centrifuge has not been used for 15 min The centrifuge lid is closed continuous cooling was stopped see Continuous cooling on p 32 In the Standby mode the following applies The display shows EP The rotor chamber is not cooled Press any key to end the standby mode 35 rri Z jenuew burje19d0 Centrifuge 5427 R Operating manual EN Maintenance 6 6 1 Operating manual gt Prepare cleaning disinfection Clean all accessible surfaces of the device and the accessories at least weekly and when contaminated Clean the rotor regularly This way the rotor is protected and the durability is prolonged Furthermore observe the notes on decontamination see Decontamination before shipment on p 39 when the device is sent to the authorized Technical Service
39. ice after the device has been moved from a cool to a E Damage to electronic components due to condensation warmer environment NOTICE 1 gt 5427 R Wait for at least 4 hours before connecting the device to the power supply Operating manual Centrifuge 5427 R compressor damage after improper transport Only switch on the centrifuge 4 hours after installation NOTICE Perform the following steps in the sequence described 1 Let the device warm up to ambient temperature 2 Check that the mains voltage and frequency match the requirements on the device type plate 3 Connect the centrifuge to the mains and switch it on using the mains power switch Display is active Lid opens automatically Remove the transport securing device of the lid latch Remove the transport securing device of the motor shaft Use the details included in the scope of delivery to check that the delivery is complete Check all parts for any transport damage Contact your dealer if any damage is found Insert the condensation water tray into the holder provided see Fig 1 on p 9 go NE DO de Ci Retain the packaging material and the transport protection device for subsequent transport or storage See also the instructions relating to transport see p 43 24 Centrifuge 5427 R Operating manual 5 Operation 5 1 Overview of operating controls Before using the Centrifuge 5427 R familiarize yourself with the
40. in the display when the rotor is running All other keys are disabled during short spin centrifugation 4 End short spin centrifugation release the short key During the braking process centrifugation time flashes in the display The centrifuge lid remains closed to maintain the sample temperature Press the open key to open the lid O During the braking process the short spin centrifugation can be restarted up to two times by pressing the short key again O The soft ramp is disabled during short spin centrifugation 34 Centrifuge 5427 R Operating manual 5 6 4 Setting the centrifugation radius If an adapter for tubes is used in a rotor this changes the centrifugation radius In the RAD menu item the parameter for the tube adapter combination has to be set Prerequisites Rotor is inserted The centrifuge has detected the rotor see Automatic rotor detection on p 28 1 Press the menu enter key 2 Select the RAD menu item using the arrow key Confirm with the menu enter key In the RAD menu item the parameters for the rotor specific tube adapter combination are available 3 Selectthe parameters for the tube adapter combination using the arrow key Confirm with the menu enter key The display shows the g force for the rotor adapter combination used in the rotor Ci Default setting If the centrifugation speed is only controlled using the g force rcf the centrifuge calculates the g force for t
41. infecting the device 1 T 8 9 Open the lid Switch off the device with the mains power switch Disconnect the power plug from the power supply Loosen the rotor nut by turning the rotor key counterclockwise Remove the rotor Clean and disinfect all accessible surfaces of the device including the power cable using a damp cloth and the recommended cleaning agents Thoroughly clean the rubber seals of the rotor chamber with water Rub the dry rubber seals with glycerine or talcum powder to prevent them from becoming brittle Other components of the device such as the lid latch motor shaft and rotor cone must not be lubricated Clean the motor shaft with a soft dry and lint free cloth Do not lubricate the motor shaft Check the motor shaft for damage Inspect the device for corrosion and damage 10 Leave the centrifuge lid open when the device is not being used 11 Only connect the device to the power supply if it is fully dry inside and out 6 2 Cleaning and disinfecting the rotor 1 Inspect the rotor and accessories for damage and corrosion Do not use any damaged rotors or accessories Clean and disinfect the rotors and accessories with the recommended cleaning agents Clean and disinfect the rotor lids ONLY QuickLock It is imperative that the sealing ring be removed during this process so that the groove below it can be thoroughly cleaned 37 rri Z Operating manual 38 Centrifuge 5427 R
42. ir freight 20 C 55 C 10 96 75 30 kPa 106 kPa 8 2 Storage Air temperature Relative humidity Atmospheric pressure In transport packaging 25 C 55 C 10 96 75 96 70 kPa 106 kPa Without transport b C 45 C 10 96 75 96 70 kPa 106 kPa 8 3 Disposal packaging In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community Within the European Community the disposal of electrical devices is regulated by national regulations based on EU Directive 2002 96 EC pertaining to waste electrical and electronic equipment WEEE According to these regulations any devices supplied after August 13 2005 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste To document this they have been marked with the following identification Because disposal regulations may differ from one country to another within the EU please contact your supplier if necessary 43 rri Z jenuew burje19d0 rri p Operating manual Centrifuge 5427 R Operating manual 9 Technical data 9 1 Power supply Centrifuge 5427 R Mains connection 230 V 50 Hz 60 Hz 120 V 50 Hz 60 Hz 100 V 50 Hz 60 Hz Current consumption 2 4 A 230 V 4 6 A 120 V 5 5 A 100 V Power consumption Maximum 550 W
43. le value for the rotor 5 4 4 Replacing the rotor Turn the rotor nut counterclockwise using the supplied rotor key Remove the rotor by lifting it vertically Vertically place another rotor on the motor shaft Insert the supplied rotor key into the rotor nut Turn rotor key clockwise until the rotor nut is firmly tightened In order to trigger the rotor detection turn the rotor counterclockwise by hand ug cB c The name of the rotor appears in the display The g force rcf and speed rpm are automatically limited to the maximum permissible value for the rotor Rotor detection can also be triggered by short spin centrifugation Press the short key until the name of the rotor appears in the display Error message after rotor change f you start centrifuging immediately after a rotor change the centrifuge has not carried out an automatic rotor detection yet The speed set for the previous rotor may exceed the maximum permitted speed for the new rotor In this case the centrifuge stops after the automatic rotor detection and displays SPEED The new maximum permitted speed appears in the display You can then restart the centrifuging with this setting or adjust the speed as necessary After each rotor change check whether the new rotor is detected by the device Check the set g force rcf or speed rpm and adjust it if necessary Centrifuge 5427 R Operating manual 5 4 5 Loading the rotor rri Z
44. mL Y 8mm 13 200 rpm UAR 5425 716 001 2 Srem 13 rri Z jenuew burje19d0 rri Z Operating manual Centrifuge 5427 R Operating manual 2 4 4 Rotors FA 45 30 11 and F 45 30 11 Rotor FA 45 30 11 Max g force 20 817 xg Aerosol tight fixed angle rotor for Max speed 14 000 rpm 30 tubes Rotor F 45 30 11 Max load adapter 30 x 3 75g tube and contents Fixed angle rotor for 30 tubes Vessels Vessel Adapters Adapter base Max g force Capacity Order no Tube diameter Max speed Vessels per adapter rotor international Centrifugation radius 5425 716 001 Vessel 20 817 xg i 1 5 mL 2 0 mL Q 11 mm 14 000 rpm 30 9 5 cm PCR tube conical 16215xg i 0 2 mL Y 6 mm 14 000 rpm 158 5425 715 005 den Vessel conical 20817xg i 0 4 mL i 6mm 14 000 rpm u 5425 717 008 sem Vessel open 18407 xg i 0 5 mL D Y 8 mm 14 000 rpm un 5425 716 001 Be Microtainers open 20817xg N 0 6 mL D 8 mm 14 000 rpm 1 30 9 5 cm Centrifuge 5427 R Operating manual 2 4 5 Rotors FA 45 48 11 and F 45 48 11 Vessels per adapter rotor international FA 45 48 11 rotor Max g force 18 213 x g outer row Aerosol tight fixed angle rotor for 16 049 x g inner row 48 tubes Max speed 12 700 rpm Fator poi Max load adapter 48 x 3 75g Fixed angle rotor for 48 tubes tube and contents Vessels Vessel Adapters Adapter base Max g force Outer row
45. n ER Salisbury Wiltshire u ns SP4 0JG Certificate of Containment Testing Containment Testing of Rotor FA 45 30 11 5409 706 101 00 in the Eppendorf 5427 R Bench Top Centrifuge Report No 200 12 B Report Prepared For Eppendorf AG Hamburg Germany Issue Date 12 September 2012 Test Summary Rotor FA 45 30 11 5409 706 101 00 was containment tested in the Eppendorf 5427 R bench top centrifuge using Annex AA of IEC 1010 2 20 The sealed rotor was shown to contain a spill within the centrifuge Report Written Wy Report Authorised By ku ox IV 7 Le csi Miss Anna Moy Name Mrs Sara Speight f Sci Health Protection Agency Microbiology Services Health Protection Porton Down Salisbury i ency Wiltshire SP4 OJG Certificate of Containment Testing Containment Testing of Rotor FA 45 24 11 5409 702 106 00 in the Eppendorf 5427 R Bench Top Centrifuge Report No 200 12 D Report Prepared For Eppendorf AG Hamburg Germany Issue Date 12 September 2012 Test Summary Rotor FA 45 24 11 5409 702 10 00 was containment tested in the Eppendorf 5427 R bench top centrifuge using Annex AA of IEC 1010 2 20 The sealed rotor was shown to contain a spill within the centrifuge Report Written U Report Authorised By Ana Moy A Name Miss Anna Moy Y Name Mrs Sara Speight Title Biosafety Scientist Title Senior Biosafety Scientist Porton Down Health Protection Agency Microbiology Servic
46. n by chemicals or other aggressive solvents d y can impair the aerosol tightness of the rotors and rotor lid WARNING gt Check the integrity of the seals of the aerosol tight rotor lids or caps before each use gt Only use aerosol tight rotor lids or caps if the seals are undamaged and clean Thinly brush the threads of the rotor lid screw with pivot grease order no Int 5810 350 050 North America 022634330 Do not apply the pivot grease to the seals gt Replace aerosol tight rotor lids and caps after 50autoclaving cycles gt For QuickLock rotor lids the seal must be replaced after 50 autoclaving cycles Never store aerosol tight rotors or buckets closed 3 6 Safety instructions located on the device Display Meaning Location N Follow the instructions in the operating manual Right side of the device en CAUTION Top of device below the centrifuge ANAYA FASTEN THE sor SUR Y Always tighten the rotor using the supplied rotor key lid CAUTION Top of device below the centrifuge Pj Close all tubes and use a rotor lid lid ALWAYS USE ROTOR LID WHEN USING SPIN COLUMNS Centrifuge 5427 R Operating manual 4 Installation 4 1 Selecting the location If an error occurs the objects in the immediate proximity of the device will be damaged In accordance with recommendations in EN 61010 2 020 leave a safety clearance of 30 cm NOTICE around the device during operation
47. nal injury is then transferred to the operator The device is not used in accordance with the operating manual The device is used outside of its intended use The device is used with accessories or consumables which are not recommended by Eppendorf The device is maintained or repaired by people not authorized by Eppendorf The user makes unauthorized changes to the device 18 Centrifuge 5427 R Operating manual 3 5 Warnings for intended use Read the operating manual and observe the following general safety instructions before using the Centrifuge 5427 R 3 5 1 Personal injury or damage to the equipment WARNING Electric shock due to damage to device or mains cable Only switch on the device if the device and mains cable are undamaged Only use devices that have been properly installed or repaired Incase of danger disconnect the device from the mains supply by pulling the power plug from the device or the mains socket or by using the isolating device intended for this purpose e g emergency stop switch in the laboratory AN LIN IN Lethal voltages inside the device Ensure that the housing is always closed and undamaged so that no parts inside the device can be contacted by accident WARNING gt Do not remove the housing of the device gt Do not allow any liquids to penetrate the inside of the housing gt Do not allow the device to be opened by anyone except s
48. ng centrifuge device settings see Fig 3 on p 26 see Fig 2 on p 25 5 Condensation water tray only 6 Emergency release see p 42 7 USB port 8 Mains power switch Only for Technical Service interfaces for Switch for switching the device on I and error analyses and software updates off 0 9 Fuse holder 10 Power connection socket Connection for supplied power cable 11 Name plate rri Z jenuew burje19d0 Centrifuge 5427 R Operating manual 2 2 Delivery package Quantity Order no Order No Description international North America 1 z Centrifuge 5427 R n See chapter Ordering Information for corresponding device version E 1 equipment and order number Fuse 1 5301 850 249 022654403 4 A 230 V 2 pieces or 5427 850 341 022654381 8 0 AT UL 120 V 100 V 2 pieces e Or 5811 352 006 022664107 10 0 AT UL 100 V 2 pieces Rotor key 1 5416 301 001 022634305 Standard 1 Power cable 1 5409 850 083 Tray for condensation water Operating Manual Centrifuge 5427 R 1 5409 900 021 5409900021 Languages EN DE FR ES IT PT 1 5409 900 030 5409900030 Languages DA Fl EL NL SV only 230 V devices 2 3 Features The high performanceCentrifuge 5427 R has a capacity of 48 x 2 mL and maximally reaches25 001 x g resp maximally 16 220 rpmYou can select from 9 different rotors to centrifuge the following tubes for your various applications Tubes 0 2 mL to 5
49. ntended for them 3 Insert sets of two tubes in the opposite bores of the rotor To ensure symmetric loading tubes that are arranged opposite each other must be of the same type and contain the same filling quantity To minimize weight differences between filled sample tubes we recommend taring with a scale This will reduce wear on the drive and reduce running noise 29 rri p Operating manual 30 Centrifuge 5427 R Operating manual 0 Spin columns For centrifuging spin columns in the rotor FA 45 24 11 Kit you can leave the tube lids open However this can only be done using the tubes provided by kit manufacturers for this purpose For reliable centrifugation you must lean the open tube lids against the edge of the rotor Ensure that the lids do not protrude past the edge of the rotor in the process then attach the matching rotor lid Loading a swing bucket rotor Prerequisites Use a rotor bucket and adapter combination that is approved by Eppendorf The carriers are sorted by weight category Carriers located opposite each other must belong to the same weight category This is laterally engraved in the groove e g 68 the last two digits in grams For repeat orders please specify the weight class Matched and checked tubes To load the rotor proceed as follows 1 Check the bucket grooves for cleanliness and lightly lubricate them with pivot grease int order no 5810 3
50. o 0 4ML Only rotor FA 45 12 17 g force rcf the radius is dependent on the 0 5ML MAX adapter used 0 6ML 1 5 2 HPLC CRYO MAX Largest radius of the used rotor LOCK On Set the centrifugation parameters permanently 5 Key lock centrifugation parameters SAFE appears in the display when the time temperature g force rcf or speed rpm temp or speed keys are pressed cannot be accidentally changed OFF v ATSET On The set run time will be counted down after pr Set start of centrifuging run time 95 of the specified g force rcf or speed rpm has been reached OFF The set time is counted down immediately a SHORT MAX Short spin centrifugation at the maximum Set the speed of the short spin centrifugation speed of the inserted rotor No SOFT function with short spin Set Short spin centrifugation with set speed centrifugation g force rcf or speed rpm Temp 8h Default setting Continuous cooling ends after Set the time limit for continuous cooling ECO Sh shut off see p 31 1h To limit continuous cooling after a run to 1 h 2h 2 h or 4 h the centrifuge lid must be opened dh and closed again after the run 00 Endless operation of continuous cooling Alarm On Switch on loudspeaker d Switch the loudspeaker on or off OFF Switch off loudspeaker x N VOL VOL1 The volume of the loudspeaker can be set to 5 Set the volume a levels VOL1 to VOL5 The loudspeaker must VOLS be switched on for the adjustment to be audible SLEEP On Stan
51. oning the following rotors including the corresponding buckets carriers and rotor lids have a maximum service life of 7 years or of the number of cycles specified in the table depending on what occurs first Rotor accessories Maximum service from commissioning onward S 24 11 AT 5427 R 5430 5430 R 75 000 cycles 7 years FA 45 48 11 5427 R 5430 5430 R 5804 5804 R 75 000 cycles 7 years 5810 5810 R FA 45 12 17 Centrifuge 5427 R 75 000 cycles 7 years QuickLock rotor lid 3 years Seals of the QuickLock rotor lid 50 autoclaving cycles Rotor lid or caps made of polycarbonate PC 50 autoclaving 3 years polypropylene PP or polyetherimide PEI cycles Adapters 1 year For all other rotors and rotor lids of this centrifuge there is no service life limit as long as the follovving requirements are met Correct use Recommended maintenance Undamaged condition The date of manufacture is stamped on the rotors in the format 03 10 2 March 2010 or on the inside of the plastic rotor lids in the form of a clock amp To ensure aerosol tightness the following applies Replace aerosol tight rotor lids and caps after 50 autoclaving cycles Replace the seal of QuickLock rotor lids after 50 autoclaving cycles 3 4 Information on product liability In the following cases the designated protection of the device may be compromised Liability for any resulting property damage or perso
52. only must be used in a safe environment such as the open environment of a ventilated laboratory or fume hood The use of substances that may contribute to a potentially explosive atmosphere is not permitted The user is responsible for making the final decision regarding the risks associated with the use of such substances 3 3 2 Maximum service life for accessories Risk of injury from chemically or mechanically damaged accessories AN Even minor scratches and cracks can lead to serious internal material amage b WARNING Protect all accessory parts from mechanical damage gt Inspect the accessories for damage before each use Replace any damaged accessories gt Do not use rotors rotor lids or buckets with signs of corrosion or mechanical damage e g deformations Do not use any accessories which are past their use by date Risk of injury due to chemically damaged rotor lids or caps Z N Transparent rotor lids or caps made from PC PP or PEI may lose their strength under the impact N of organic solvents e g phenol chloroform N EROMONI If rotor lids or caps have come into contact with organic solvents they should be cleaned immediately gt Regularly check the rotor lids and caps for damages and cracks gt Replace rotor lids or caps which show cracks or milky stains immediately 17 rri Z Operating manual Centrifuge 5427 R Operating manual Beginning with the first commissi
53. ration can shorten the service life of the compressor The rotor chamber may freeze 1 Press the menu enter key 2 Select TEMP using the arrow key Confirm with the menu enter key 3 Select oo using the arrow key Confirm with the menu enter key Ending continuous cooling 4 Open the centrifuge lid to end continuous cooling jenuew Burjeuado Risk from incorrectly loaded rotors and damaged overloaded tubes gt Before commencing centrifugation follow the safety instructions relating to risks from asymmetrically loaded and or overloaded rotors and from overloaded damaged and or open CAUTION tubes see Warnings for intended use on p 19 A Risk of injury from improperly attached rotors and rotor lids AN Only centrifuge with rotor and rotor lid firmly tightened b WARNING gt If unusual noises occur when the centrifuge starts the rotor or the rotor lid may not be properly secured Immediately press the start stop key to stop centrifuging Before using the Centrifuge 5427 R for the first time familiarize yourself with the operating controls and the display see Overview of operating controls on p 25 Each of the centrifuging variants described here must be preceded by the preparation described above see Preparing for centrifugation on p 28 Please also note the instructions on cooling see p 31 5 6 1 Centrifuging with preset time Perform the following steps in the sequence described Use
54. re To prevent the rotor chamber from freezing or condensation from forming the temperature does not go below 4 C irrespective of the set temperature f the rotor stops temperature control is slower than during centrifugation or a temperature control run ECO shut off Continuous cooling is switched off if the centrifuge is not used for longer than the preset time The centrifuge switches to standby mode Default setting Continuous cooling ends after 8 h Continuous cooling can be limited to 1 h 2 h or 4 h ECO shut off can be switched off continuous cooling set to endless operation Limit continuous cooling to 1 h 2 h 4 h 1 Press the menu enter key 2 Select TEMP using the arrow key Confirm with the menu enter key 3 Select 1 h 2 h 4 h using the arrow key Confirm with the menu enter key 4 To activate ECO shut off after 1 h 2 h or 4 h open and close again the centrifuge lid after the end of the run The device switches to standby mode after 1 h 2 h or 4 h The display shows EP O f the centrifuge lid is not opened and closed again after the run ECO shut off engages after 8 h default setting Open the centrifuge lid to end continuous cooling early 32 Centrifuge 5427 R Operating manual 5 5 6 Endless operation of continuous cooling 5 6 Centrifuging rri Z The ECO shut off function can be switched off Continuous cooling is changed to endless operation Endless ope
55. ressive chemicals including strong and weak alkali strong acids solutions with mercury copper and other heavy metal ions halogenated hydrocarbons concentrated saline solutions and phenol gt Due to the manufacturing process color variations may occur on rotors marked coated These color variations do not effect service life or resistance to chemicals If handled incorrectly the rotor can fall over The buckets of the S 24 11 AT may not be used as a handle NOTICE gt Before moving the rotor remove the buckets gt Always grip the rotor on the rotor cross using both hands 3 5 4 Extreme strain on the centrifuging tubes y Risk of injury from overloaded tubes y gt Note the loading limits specified by the tube manufacturer CAUTION gt Only use tubes which are approved by the manufacturer for the required rcf Risk from damaged tubes Damaged tubes must not be used as this could cause further damage to the device and the NOTICE accessories and loss of the samples gt Before use visually check all of the tubes for damage Risk from open tube lids Open tube lids can break off during centrifugation and damage both the rotor and the centrifuge NOTICE gt Carefully seal all tube lids before centrifuging Exception Note the information on the centrifugation of spin columns in the rotor FA 45 24 11 Kit see Preparing for centrifugation on p 28 Hazard to plastic tubes from organic solvents The d
56. shock A Crushing Hazard point Material damage AN 1 2 2 Danger levels DANGER Will lead to severe injuries or death WARNING May lead to severe injuries or death CAUTION May lead to light to moderate injuries NOTICE May lead to material damage 1 3 Symbols used Depiction Meaning You are requested to perform an action a Perform these actions in the sequence described 2 List Text Terms and key names from the software e References useful information rri Z jenuew burje19d0 rri Z Operating manual Centrifuge 5427 R Operating manual 1 4 Abbreviations used NN PCR RCF rpm UV Mean sea level MSL Polymerase chain reaction Relative centrifugal force g force in m s Revolutions per minute in rpm Ultraviolet radiation Centrifuge 5427 R Operating manual 2 Product description 2 1 Main illustration The Centrifuge 5427 R diagram can also be found on the front fold out page ENDS SES N SS Nx N E 3 NS zu 4 A i 5 centrituge 54277 y Fig 1 Front and rear view of the Centrifuge 5427 R 1 Centrifuge lid 2 Monitoring glass Visual control for rotor stop or speed control option using stroboscope 3 Display 4 Control panel Display of centrifugation parameters and Keys and dials for operati
57. the use of aggressive chemicals gt Do not use any aggressive chemicals on the device or its accessories such as strong and weak bases strong acids acetone formaldehyde halogenated hydrocarbons or phenol gt Ifthe device has been contaminated by aggressive chemicals immediately clean it by means of a mild cleaning agent NOTICE 36 Corrosion from aggressive cleaning agents and disinfectants Do not use corrosive cleaning agents aggressive solvents or abrasive polishes gt Do not incubate the accessories in aggressive cleaning agents or disinfectants for a longer period of time Centrifuge 5427 R Operating manual rri Z Damage from UV and other high energy radiation gt Do not use UV beta gamma or any other high energy radiation for disinfecting NOTICE gt Avoid storage in areas with strong UV radiation O Autoclaving All rotors rotor lids and adapters can be autoclaved 121 C 20 min O Aerosol tightness Check that the seals are intact before use jenuew Burjeuado Only QuickLock rotor lid Replace the sealing ring in the lid groove when it becomes worn The sealing rings require regular care to protect the rotors Aerosol tight rotors should never be stored with lids screwed on In order to prevent damage lightly lubricate the lid thread of the aerosol tight rotors with pivot grease order no Int 5810 350 050 North America 022634330 6 2 1 Cleaning and dis
58. time to set the run time Use temp to set the temperature With speed set the g force rcf or speed rpm Pon gt Press start stop to start the centrifugation During centrifugation blinks in the display when the rotor is running The current actual temperature will be displayed The current g force rcf resp speed rpm of the rotor is displayed The fast temp open short keys and the device menu are blocked during centrifugation The total run time temperature and speed rpm can be changed during the run The display can be switched between g force rcf and speed rpm You can also terminate the centrifugation before the set run time has elapsed by pressing the start stop key End of centrifugation After completion of the set time the centrifuge stops automatically During braking the elapsed centrifugation time is displayed flashing When the rotor stops a signal tone is heard The centrifuge lid remains closed to maintain the sample temperature You can open it by pressing the open key 33 rri p Operating manual Centrifuge 5427 R Operating manual Ci During the run you can modify the total run time the temperature and the g force rcf speed rpm The values on the display blink while changes are being made The new parameters are adopted immediately If the time is changed during a run the elapsed time will be subtracted from this value Please note that the shortest new total
59. tion 7 Set centrifugation speed 8 Set the temperature 9 Adjust centrifugation time 10 Start FastTemp temperature control run 11 Select menu item 1 2 3 4 6 7 1 1 El L4 em IT ILL 5 La LILILILI 9 8 1 Centrifugation time 2 Key lock status 3 Status of ATSET function 4 Temperature 5 Softramp 6 g force rcf or speed rpm 7 Centrifuge status 8 Display of centrifugation speed rcf or rom 9 Loudspeaker status Rotor code icu p pie iy E x LI All Eppendorf rotors are identified using a simple alphanumeric format that represents the technical specifications in a uniform series of letters and numbers Aerosol tight version F A 45 30 11 Number of bores S 24 11 AT Number of bores Aerosol tight version Centrifuge 5427 R Operating manual Table of contents User instructions 0 0 cece eee heh hh hne nn eee eee eee eee eee 7 1 1 Using this marnual 34 02 2 rero era PA oes dr UNE E Roca a omo ag argon Rede a 7 1 2 Danger symbols and danger levels aaa 7 1 2 1 Danger symbols 22g d rere BARDS RE RUE no EROR E ee RUE E eed eke en 7 1 2 2 Danger levels pi k epe d kai A A Ted Reg 7 L3 Symibols sed i ia onte heen ptores dh fuese d NR ER Ted Leas he 7 1 4 Abbrevi tionsiused ss en rh oS an ed Da Sh ee ea aged ie dus bre ht 8 Product description scia in tenis A E ee RUE RO A 9 2 1 Main lllustration sha s anexo Re x ede eee oa oe peu fornia A e RR QR 9 2 2
60. urg Germany Tel 49 40 53801 0 Fax 49 40 538 01 556 E mail eppendorf eppendorf com Eppendorf North America Inc 102 Motor Parkway Hauppauge N Y 11788 5178 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E mail info eppendorf com Application Support Europe Tel 49 1803 666 789 Preis je nach Tarif im Ausland 9 ct min aus dem dt Festnetz Mobilfunkh chstpreis 42 ct min support eppendorf com North America Tel 1 800 645 3050 E mail techserv eppendorf com Asia Pacific Tel 60 3 8023 6869 E mail support_asiapacific eppendorf com
61. ws Err 18 Centrifugation is stopped automatically 31 Centrifuge 5427 R Operating manual 5 5 4 Start FastTemp EN With the FastTemp function you can immediately start a temperature run without samples at rotor specific or temperature specific speeds This will quickly bring the rotor chamber including rotor and adapter up to the set target temperature Prerequisites The centrifuge is switched on Rotor and rotor lid are correctly mounted The centrifuge lid is closed The temperature and g force rcf or speed rpm have been set for the upcoming centrifugation see Centrifuging on p 33 Operating manual 1 Press the fast temp key The display shows e FT Actual temperature in the rotor chamber g force rcf or speed rpm The temperature control run FastTemp automatically ends when the target temperature has been reached A periodic signal tone sounds 2 Press the start stop key to end the temperature control run early O The centrifuge only stops the run once the rotor has reached the set temperature Therefore there may be a delay of approx 15 min rotor S 24 11 AT 30 min between the display indicating the set temperature has been reached and the automatic end of the temperature control run 5 5 5 Continuous cooling Continuous cooling Continuous cooling maintains the rotor chamber at the set temperature if the rotor stops During continuous cooling the display shows the set temperatu

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